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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 9, Issue 4 - Dec 2003
Volume 9, Issue 3 - Sep 2003
Volume 9, Issue 2 - Jun 2003
Volume 9, Issue 1 - Mar 2003
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Chinese yam necrotic mosaic virus Isolated from Chinese Yam in Korea
Kang, Dong-Kyoon ; Kondo, Toru ; Shin, Jong-Hee ; Shin, Hye-Young ; Sung, Jung-Hyun ; Kang, Sang-Gu ; Chang, Moo-Ung ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 107~115
DOI : 10.5423/RPD.2003.9.3.107
Chinese yam (Dioscorea opposita cv. Jang-Ma) plants showing necrotic mosaic symptom were collected from their growing fields in Andong, Euisong, Gunwi and Daegu, Korea. Direct negative stainning method by electron microscope showed filamentous particles of about 660 nm in length. Immunosorbent electron microscopy (ISEM) revealed filamentous particles of 660nm decorated with antiserum of Chinese yam necrotic mosaic virus (ChYNMV). The virues purified partially were used to isolate viral RNA as template for RT-PCR to amplify about 1.2 kbp of 3`-terminal region (coat protein, 3`-UTR) with ChYNMV specific and oligo-dT primers. Amino acids sequences of amplified CP genes revealed that the viruses shared 97.9% similarity with ChYNMV (AB044386) wh ich was known as the member of Macluravirus. So the viruses from Chinese yam (D. opposita cv. Jang-Ma) plants were identified as ChYNMV. Comparing the CP amion acid sequences of ChYNMV strains with other macluraviruses such as Cardamon mosaic virus (CdMV), Narcissus latent virus (NLV) and Maclura mosaic virus (MacMV) revealed that N-terminal was the most varialbe region and conserved regions were present within the genus Macluravirus.
Nested PCR Detection of Pseudomonas syringae pv. actinidiae, the Causal Bacterium
Jung, Jae-Sung ; Han, Hyo-Shim ; Jo, Youn-Seob ; Koh, Young-Jin ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 116~120
DOI : 10.5423/RPD.2003.9.3.116
A PCR method that combines biological and enzymatic amplification of PCR targets was developed for the detection of Pseudomonas syringae pv. actinidiae on kiwifruit leaves. A nested PCR was performed with primers designes from the coding sequence of the cfl gene, which is involved in production of the phytotoxin coronatine. The first and second primer sets efficiently amplified expected 665 and 310-bp fragments, respectively. With two successive amplifications, as few as 20 CFU/ml of P. syringae pv. actinidiae could be detected on ethidium bromide-stained agarose gel. Leaf samples were collected from 4 kiwifruit trees showing yellow halo spots on leaves and incubated in pepton-sucrose broth for 12 h at
C before PCR amplification. Positive detection was obtained with one sample, which was proved as a diseased plant in the next spring.
Occurrence of Garlic Rust in Southern Regions of Korea
Lee, Dong-Hyun ; Hur, Jae-Seoun ; Koh, Young-Jin ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 121~124
DOI : 10.5423/RPD.2003.9.3.121
Occurrence of rust on 2 representative garlic cultivars Daeseo and N므애 in major cultivating regions of Goheung-gun, Jeonnam and Namhae-gun, Gyeongnam was investigated during 1998-1999. Rust disease severities of garlic ranging from 1.2 to 85.8 were quite different according to the investigating years and cultivars. Relatively high rust severities of garlic were observed in 1998 compared with 1999. This might be due to remarkably frequent rainfalls and high humidities during the growing seasons of garlic in 1998. Rust occurred severely on cultivar Daeseo but not on cultivar Namdo. This shows quite different susceptibility or resistance to rust among the cultivars of garlic. Increase of rust severities resulted in remarkable decrease in yields of garlic. It is assumed that rust might cause serious damages in the cultivation of garlic.
Rust of Iris nertschinskia Caused by Puccinia iridis
Kwon, Jin-Hyeuk ; Park, Chang-Seok ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 125~127
DOI : 10.5423/RPD.2003.9.3.125
Rust disease was occurred on Iris in several farmer`s fields fields located in Hoehwa-myon, Goseong-gun, Gyeong-nam province in Korea. The typical symptoms of the disease appeared first as small, yellowish spot on leaves. The spots then turn brown, their edges rised slightly, and powdery mass of yellowish brown spores revealed on the lesions. Severely infected leaves were blighted and eventually died. Urediniospores were yellowish brown to reddish brown in color, obobid to obovoid in shape and 22~30
m in size. Teliospores were chestnet-brown in color, oblong or clavate in shape and 32~54
in size. The causal fungus was identified as Puccinia iridis, based on morphological characteristics. This is the first report on the rust of Iris nertschinskia by Puccinia iridis in Korea.
Rust of safflower (Carthamus thinctorius) caused by Puccinia carthammi
Park, Kyeng-Seuk ; Lee, Soon-Gu ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 128~130
DOI : 10.5423/RPD.2003.9.3.128
Rust diseases were found on safflower (Carthamus thinctorius) fields located in Euisong-gun Gyengbuk province in Korea. The infection rate of the disease in surveyed area was about 15% in 2001. The typical symptoms of the disease appeared first as small white spots on the leaf and turn brownish, dark brown spores revealed on them. Uredospores were light brown in color, ellipsoid or spherical in shape and 21~23
in size. Teliospores were chestnut-brown in color, ellipsoid or ovoid in shape and 35~40
in size. Pedicels were non-color of limpidness in color and 15~30
in size. The causal fungus was identified as Puccinia carthami, based on morphological charateristics. This is first report on the rust of safflower caused by Puccinia carthami in Korea.
Influence of Temperature, Wetness Duration and Fungicides on Fungal Growth and Disease Progress of Soybean Anthracnose Caused by Colletotrichum spp.
Oh, Jeung-Haing ; Kim, Gyu-Hong ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 131~136
DOI : 10.5423/RPD.2003.9.3.131
The effects of temperature, duration of wetness period, and fungicides on the spore germination, appressorium formation, acervulus formation and lesion development by Colletotrichum spp., cause of soybean anthracnose, and their pathogenicity were assessed in controlled environment. C. gloeosporioides was highly pathogenic on inoculated soybean seeds as high as C. truncatum, whereas remarkably low on the soybean leaves. Spore germination, appressorium formation and mycelial growth of C. gloeosporioides were best at
, but C. truncatum was best at
. It has also done at
, even though it was much retarded. C. truncatum showed high sensitivity to the fungicides, fluazinam and benomyl, meanshile C. gloeosporioides showed to fluazinam and triflumizole. At least 8 hrs. of wetness period was requird for the pathogen to develop lesions at
. When the wetness period was 32 hrs. lesion size of was larger at
, however it was traceable at
. Different sensitivity of Colletotrichum spp. to fungicides suggests that proper fungicide is required to effective control of soybean anthracnose ingected multiply with Colletotrichum spp.
Development Characteristics of Potato Common Scab in Jeju Inland
Hong, Soon-Yeong ; Kang, Yong-Kil ; Hahm, Young-Il ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 137~144
DOI : 10.5423/RPD.2003.9.3.137
This study was conducted to clarify developmental characteristics of scab in potato fields in Jeju island, Korea from 1995 to 1999. Occurrence of potato scab increased with repeated cultivaton of potato and high soil pH in the fields. Incidence of the disease was high as 54.8％ in the repeatedly cultivated potato fields but relatively low as 20.8~26.3% in the non-cultivated fields and in the fields where barley and Chinese cabbage were formerly cultivated. A total of 66 isolates were obtained from the diseased patato tubers and identified as Streptomyces scabies, S.turgidiscabies and S. acidiscabies. The isolation frequency of each Streptomyces species was 37.7%, 14.8% and 18.0%, respectively. The optimum temperature for mycelial growth of the Streptomyces spp. was
, and the optimum pH for that 6~7.
Gray Mold of Kalanchoe (Kalanchoe blossfeldiana) Caused by Botrytis cinerea in Korea
Kwon, Jin-Hyeuk ; Park, Chang-Seuk ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 145~148
DOI : 10.5423/RPD.2003.9.3.145
In April of 2002, the gray mold disease occurred severely on kalanchoe (Kalanch oe blossfeldiana) grown in a nursery in Daesan-myon, Changwon City, Korea. The lesions were started with water-soaked spot and the leaves discolored from the tip and became gray or dark and gradully expanded to whole leaf. The conidia and mycelia of pathogen appearedd on flower, flower stalk and stem. Conidia were gray, 1-celled, mostly ellipsoid or ovoid in shape and were 8~17
in size. Conidiophores were 13~34
in size. The sclerotia were formed abundantly on potato-dextrose agar. The optimum temperature for sclerotial formation was
. Pathogenicity of the causal organism was proved on Kalanchoe blossfeldiana according to Koch,s postulate. The causal organism was identified as Botrytis cinerea based on mycological characteristics. This is the first report on gray mold of Kalanchoe(Kalanchoe blossfeldiana) caused by Botrytis cinerea in Korea.
Black Leaf Spot of Dendrobium phalaenopsis Caused by Fusarium moniliforme
Lee, Dong-Hyun ; Hur, Jae-Seoun ; Koh, Young-Jin ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 149~152
DOI : 10.5423/RPD.2003.9.3.149
Black leaf spot disease occurred on Dendrobium phalaenopsis grown in farmer`s fields located in Bonggangmyon, Gwangyang-eup, Jeonnam, Korea. Black small spots occurred on leaves at initial stage of infection and the infected leaves turned yellow from the tip. The yellow leaves were falled, resulting in stem blighting or eventual death of the entire plant. White mycelial colony of the causal fungus grown on potato dextrose agarturned dark violet later and optimum temperature for the mycelial growth was
. The causal fungus isolated from the black leaf spot on D. phalaenopsis was identified as Fusarium moniliforme based on the mycological characteristics and pathogenicity. The fungus also caused same symptoms on leaves of Phalaenopsis sp. and Cymbidium sp. as well sa D. phalaenopsis by wound inoculation. This is the first report on black leaf spot of D. phalaenopsis caused by F.moniliforme in Korea.
Characterization of the Causal Fungus of Citrus Melanose, Diaphorthe citri Isolated from Blighted Twigs of Citrus in Jeju
Kwon, Hyeog-Mo ; Nam, Ki-Woong ; Kim, Kwang-Sik ; Kim, Dong-Hwan ; Lee, Seong-Chan ; Hyeon, Jae-Wook ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 153~158
DOI : 10.5423/RPD.2003.9.3.153
Mycological characteristics of Phomopsis citri isolates obtained from blighted twigs of citrus cultivation areas in Jeju island were all identified as Phomopsis citri known as the anamorph stage of Diaporthe citri. Pycnidia the isolates sized from 112.2 to 614.4
) and shaped conical to lenticulata in black. Two types of phialospores,
-spores were observed from pycnidia. The
-spores were hyaline, unicellula, fusiform to ellipsoidal and sized 4.7~8.7 1.9
-spores were hyaline, unicellula, filiform, curved and often strongly hooked and sized 13.2~27.1
). All isolates grew well and produced abundant pycnidia and spore horns on PDA. In addition, all isolates showed strong pathogenicity to citrus fruit inducing melanose symptoms when artificially inoculated with the pycnidial spores.
Leaf Spot of safflower (Carthamus thinctorius) caused by Alternaria carthami and A. alternata
Park, Kyeng-Seuk ; Lee, Soon-Gu ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 159~161
DOI : 10.5423/RPD.2003.9.3.159
Leaf spot diseases were found on safflower (Carthamus thinctorius) leaf in several fields located in Euisong-Gun, Gyengbuk province in Korea. The infected leaf rate of the diseases in surveyed area were different each years from 2001 to 2003 such as about 5%, 10%, and 30%, repectively. The typicla symptoms of diseases appeared first as small, light brown spots on leaves. And then spot turn dark brown and magnified. And yellow decoloration zone appeared surrounding dark brown spots. Mycological characteristics of Alternaria carthami, isolated from safflower mature leaf spot were dark gray colonies, conidiophores simple erect, septated, 40~80
length; conidia solitary, straight, body size fo without beak length 40~100
thick, Number of transverse septa 4~10 and longitudinal septa 4~7 ; beak with 2~4 septa, 30~65
length. And A. alternata, isolated from old leaf were gray~dark gray colonies, conidiophores simple or branched, 20~110
length; conidia long chaines, short conical or cylindrical beak, 2~6 transverse septa and several longitudinal septa, body size of without beak length 30~60
thick ; beak length 5~35
. These are the first report on the leaf spot of safflower caused by Alternaria carthami, A alternata in Korea.
Sporulation of Cercospora capsici causing Cercospora leaf spot of Pepper
Lim Yang-Sook ; Kim, Byung-Soo ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 162~165
DOI : 10.5423/RPD.2003.9.3.162
High sporulation method and cultural characteristics of Cercospora capsici causing Cercospora leaf spot of pepper were examined. Optimum temperature for mycelial growth of Cercospora capsici was
. The fungus did not grow below
. Optimum pH for mycelial growth was pH 4.0~pH 8.0. Mycelial growth was not influenced by light. C. capsici sporulated well on pepper leaf agar (5g/l). A standard method of sporulation established was as follows. The mycelial plugs were ground with some water in motar with pestle. The mycelial suspension was smeared on the surface of medium and incubated for 2~3 days at
. The culture surface was lightly scraped with a brush after adding 1 ml of sterile water to stimulate sporulation and further incubated for 2~3 days.
Optimal Conditions for Resistance Screening of Cercospora leaf spot by Cercospora capsici on Pepper
Lim, Yang-Sook ; Kim, Byung-Soo ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 166~169
DOI : 10.5423/RPD.2003.9.3.166
Occurrence of cercospora leaf spot by Cercospora capsici is assamed to remarkbly cause the yield reduction through the leaf spot, yellowing of leaves and early defoliation in pepper cultivation. To develop resistant cultivar, optimal screening conditions of pepper against cercospora leaf spot were studied. Optimal screening conditions were 3.4
conidia/ml conidial suspension were sprayed on to the leaves of 30~40 days old pepper seedlings, incubated at 15~
(day/night) and examined 20 days after inoculation.
Screening of Fungicies for the Control of Postharvest Fruit Rots of Kiwifruit
Koh, Young-Jin ; Lee, Jae-Goon ; Hur, Jae-Seoun ; Park, Dong-Man ; Jung, Jae-Sung ; Yu, Yong-Man ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 170~173
DOI : 10.5423/RPD.2003.9.3.170
For the effective chemical control to minimize infection of storage pathogens on kiwifruit in the field, this study was conducted to screen alternative fungicides which could be substituted for the fungicides Benomyl WP and Thiophanate-methyl 제 registered for the control of postharvest fruit rats of kiwifruit in Korea. Among the 8 fungicides tested, Tebuconazole WP, Iprodione WP and Flusilazole WP showed higher inhibitory effects on themycelial growths of 3 major pathogens of postharvest fruit rots, Botryosphaeria dothidea, Diaporthe actinidiae and Botrytis cinerea on potato dextrose agar. They also showed control efficiencies as high as those of Benomyl WP and Thiophanate-methyl WP on postharvest fruit rots in the field. They might be good candidates for fungicieds for the control of postharvest fruit rots of kiwifruit.
Biological Control of Soybean Anthracnose by Pseudomonas sp.
Oh, Jeung-Haing ; Kim, Kyu-Hong ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 174~178
DOI : 10.5423/RPD.2003.9.3.174
Pseudomonas sp. antagonistic to Colletotrichum truncatum and C. gloesporioies was selected as a biological control agent for soybean anthracnose. Pseudomonas sp. inhibited the mycelial growth of pathogens effectively as the funhicides such as benomyl and fluazinam in vitro tests without any adverse effects on soybean. Seed treatment with Pseudomonas sp. increased emergence rate of soybean seeds significantly after inoculaton with C. truncatum. When the suspension of Pseudomonas sp. was sprayed on soybean plants, the control efficacy was not different from that of fungicides, benomyl and fluazinam two weeks after treatment, however the efficacy did not last long enough after three weeks.
Control of Potato Late blight (Phytophthora infestans) with Postassium Phosphonate
Hong, Soon-Yeong ; Lee, Kwang-Seok ; Kang, Yong-Kil ; Jee, Hyeong-Jin ;
Research in Plant Disease, volume 9, issue 3, 2003, Pages 179~182
DOI : 10.5423/RPD.2003.9.3.179
Effect of potassium phosphonate on control of potato late blight was evaluated at two fields in Jeju island. The chemical showed 82.5% control value in field located at low seacoast with 100 m elevation, while dimethomorph copper oxychloride showed 75.9 % control value. However, its control value was only 40% in another field located at mid-hilly area with 300 m elevation, in which environmental conditions of high hummudity and often rainfall were favorable to the disease development. Application intervals of the phosphonate from 7 to 15 effectively suppressed the disease and did not show statistically different control values among the spraying intervals. Results indicated that phosphonate similarly or more effectively controls potato late blight than fungicide, however, its control value could be vared according to enviromental conditions for the disease development and 15-d spraying intervals ware sufficient to suppress ther disease.