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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Life Science
Journal Basic Information
Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 10, Issue 6 - Dec 2000
Volume 10, Issue 5 - Oct 2000
Volume 10, Issue 4 - Aug 2000
Volume 10, Issue 3 - Jun 2000
Volume 10, Issue 2 - Apr 2000
Volume 10, Issue 1 - Feb 2000
Selecting the target year
Effects of Some Temperatures on Larval Development, Adult Longevity and Oviposition of the Beet Armyworm, Spodoptera exigua Hubner
Journal of Life Science, volume 10, issue 1, 2000, Pages 1~6
Larval development, adult longevity and oviposition of the beet armyworm, Spodoptera exigua H bner was studied at 16, 20, 25 and 3
with a 16L: 8D photoperiod. Egg hatchability of S. exigua was the highest at 3
and reduced with decreasing temperature. Egg period was the shortest at 3
and prolonged with decreasing temperature. Percent pupation was became higher at higher temperature form 23.2% to 94.0%. Developmental period of larva was the shortest at 3
, but extended with decreasing temperature. Adult emergence ratio was the highest at 3
and reduced with decreasing temperature. The mean pupal period was 35.2, 14.4, 7.8 and 5.0 days at 16, 20, 25 and 3
, respectively. Relationships between constant temperature and the developmental velocity of egg, larva and pupa were linear. Developmental threshold temperature seems to be 13.3 8
for egg, 14.02
for larva, 14.09
for pupa, and 13.84
for egg the adult. Estimated value of the total effective temperature for completing each stage was 36.89 day-degree for egg, 155.72 day-degree for larva, 79.20 day-degree for pupa, and 273.41 day-degree for egg to adult. Female longevity varied to temperature from 11.4 days at 16
to 5.8 days at 3
, and male longevity was longer than that of female. Preoviposition period was the shortest at 3
, but prolonged with decreasing temperature. It was about 2.7 times longer at 16
than that at 3
. Number of eggs per female was the highest at
, followed by 20, 30 and 16
The Effectsof Daucus carota L. Extracts on Serum Lipid and Antioxidative Enzyme Activity in Ovariectomized Rats
Journal of Life Science, volume 10, issue 1, 2000, Pages 7~13
The purpose of this study was to investigate whether the extracts of Daucus carota L. could lead to a beneficial outcomes on serum lipid and antioxidative enzyme activity in ovariectomized rats. Sprague-Dawley rats were randomly assigned to one control and three diet groups; carrot seed, carrot root and estrogen after ovariectomy operation. Experimental diets were fed for 8 weeks. The GOT activity was decreased in the carrot extracts treated group than in the control group. The carrot seed extracts treated group showed the lowest SOD and catalase activities compare to other groups. Serum total cholesterol and LDL cholesterol decreased in the carrot seed extracts group than in the control group. From these results, it suggest that carrot seed extracts positively influenced on serum lipid and antioxidative enzyme activity.
Development of biological processes for the removal of assimilable organic carbon from potable water
Journal of Life Science, volume 10, issue 1, 2000, Pages 14~21
The experiments were performed using both batch and continuous column reactors. Batch biodegradation studies were performed under aerobic conditions to determine the biodegradable fraction of the natural organic matter (NOM) source. NOM source was evaluated for its biodegradability at three different UV irradiation conditions and compared to its biodegradability without UV irradiation. In continuous experiments, system operating parameters of empty bed contact time (EBCT), recycle ratio, and influent concentration affected the extent of biofiltration in the biofilters. The effluent UV254/DOC ratios fro the biologically active columns were consistently lower than the influent values, which indicated that the dissolved organic carbon (DOC) removed by biodegradation was not a significant part of the UV-absorbable material. The increase in UV254/DOC ratio was caused by the DOC decrease across the biofilter because there was essentially no difference between the feed and effluent UV254 absorbance values over time. The results of this research showed that biofiltration was an effective method for removing the biodegradable fraction of NOM from water supplies.
Persistence of Stem-like Cells in Glandular Structures in Mammary Cell Grafts
;;Kelly H. Clifton;;
Journal of Life Science, volume 10, issue 1, 2000, Pages 22~36
The mammary gland contains a subpopulation of epithelial cells with large proliferative potentials which are the likely targets for carcinogens. These clonogenic cells can proliferate and differentiate into functional glandular structures. Multicellular secretory alveolar units (AU) develop from these clonogens in grafts of monodispersed rat mammary epithelial cells (RMEC) in gland-free mammary fat pads in intact recipient F344 rats co-grafted with mammotropic hormone-secreting pituitary tumors (MtT F4). Multicellular nonsecretory ductal units (DU) develop in grafts of monodispersed RMEC in gland-free fat pads in adrenalectomized recipient WF rats co-grafted with MtT W10. However, this effect were reversed by hydrocortisone replacement therapy. RMEC were isolated from appropriate donor rats as monodispersed mixed cells or, alternatively, RNA＋ cells were sorted by flow cytometry of mixed RMEC stained with FITC-RNA and PE-anti-Thy-1.1 monoclonal antibody. We grafted mixed or sorted PNA+ cells in gland-free mammary fat pads in recipient rats that were endocrinologically manipulated to induce AU or DU. Cells were also isolated from these AU or DU as mixed or sorted RNA＋ cells and sub-transplanted in recipient rats treated appropriately to induce AU or DU, respectively. Cells obtained from AU in grafts gave rise to clonal AU and from DU in grafts to DU on sub-transplantation in appropriate recipients. When adrenalectomized recipient WF rats co-grafted with MtT W10 received daily subcutaneous injections of hydrocortisone for periods of 21 days following the PHA＋ cell transplantation, AU, instead of DU, were developed. The histologies of these secondary AU and DU were not different from those of the primary AU and DU. Casein and laminin proteins were demonstrated by immunocytochemical staining of primary and secondary AU. Electron micrographs also demonstrated that AU were composed of secretory cells with milk protein in the cytoplasm. DU were composed of little or non-secretory ductal epithelial cells. These AU and DU also secreted large amounts of lipids. Clonogenic cells were more common in DU than in AU. Thus, AU and DU contain persistent subpopulations of clonogenic stem-like cells.
Isolation and Characterization of Mammary FpithelialStem Cells in Culture
;;Kelly H. Clifton;;
Journal of Life Science, volume 10, issue 1, 2000, Pages 37~44
The mammary gland contains a subpopulation of epithelial cells with large proliferative potentials which are the likely targets for carcinogens. These clonogenic cells can proliferate and differentiate into functional glandular structures. Rat mammary epithelial cells (RMEC) were isolated and characterized in vitro. By flow cytometry of RMEC stained with fluorescein isothiocyanate-peanut agglutinin(PNA) and phycoerythrin anti-Thy-1.1 monoclonal antibody, it was possible to four cell subpopulations from 7-8 week old F344 female rat mammary glands: cells negative to both reagents (B-), PNA-positive cells (PNA+), Thy-1.1-positive cells (Thy-1.1+), and cells positive to both reagents (B+). When single PNA+ cells were isolated and cultured in Matrigel with irradiated (∼50 Gray) 3T3 fibroblast feeder layer, they gave rise to multicellular clonal structures of three types: alveolar, foamy alveolar, and squamous colonies. The developed structures were similar to the mammary glands in vivo. These results suggest that some of PNA+ cells possesses many of the characteristics of multipotent clonogenic stem-like cells.
Ovarian Development and Yolk Protein of the Mushroom Fly, Coboldia fuscipes (Diptera: Scatopsidae)
Journal of Life Science, volume 10, issue 1, 2000, Pages 45~51
Ovarian development and yolk protein (YP) of mushroom fly, Coboldia fuscipes, were characterized. C. fuscipes has a pair of ovaries, composed of 130∼140 ovarioles, respectively. Ovarian development begins at 1 day of pupa, and growth of the ovaries continued to become a matured shape at 1 day after emergence. The YP of C. fuscipes identified by SDS-PAGE analysis revealed that the protein is composed of three subunits, designated YP1 (61 kDa), YPS(50 kDa), and YP3 (47 kDa). These three subunits of YP gradually decreased during embryogenesis. The YP was first detected in the 3 day-old pupal ovary and was continually detected up to 2 day-old adult, but not in the hemolymph. Furthermore, Western blot analysis of male and female adult hemolymph and ovary revealed that the antibodies against YP1, YP2, and YP3 reacted with three YP bands in ovary and egg extract, respectively. However, this reactivity was not observed in the male and female hemolymps. Therefore, it is assumed that the UP of C. fuscipes is synthesized in the ovary at 3 days after pupation.
Some Aspects to the in vivo Nitrate Reductase Activity in Carex species
Journal of Life Science, volume 10, issue 1, 2000, Pages 52~60
Up to now, there have been done much efforts in regard to nitrate reductase activity (NRA) of dicotyledonous herbs and important crop monocotyledons, but few to wild plants having canopy structure such as Carex. The objective of the present study are to determine: a) the optimum in vivo NR assay conditions for leaf samples of Carex species, b) changes of NRA according to section within leaf and leaf ages, c) diurnal variations. Optimized assay media of each Carex species were determined. NRA of C. rostrata adapted to oligotrophic habitats is readily saturated at lower substrate concentration than those of C. distans and C. gracilis, adapted to meso- and eutrophic habitats, respectively. All Carex species investigated have higher NRA in leaves than in roots. NRA of all species showed maximal values at the middle section of each leaf and in the youngest fully expanded leaves. Compared to C. gracilis, NR in leaves of C. distans was adapted readily to the light period. On the whole, Carex showed rather delayed diurnal variation. Even if the in vivo nitrate reductase assay based on nitrite estimation does not give an accurate estimation of total nitrate reduced, it still serves as a useful tool to find out relative differences in varying environmental conditions. Additionally, in vivo RNA measurements are helpful to understand nitrate reduction and basic nitrogen metabolism of Carex species having different canopy structure.
Shelf-Life Extension of Cooked Squid Using Modified Atmosphere Packaging
Journal of Life Science, volume 10, issue 1, 2000, Pages 61~67
An experiment was conducted for extension of shelf-life of cooked squid (Todarodes pacificus) using modified atmosphere packaging. The products were packed in a Ny/PE/LLDPE (0.015/0.045/0.040 mm) laminated film with air, vacuum, CO2 gas, and N2 gas, respectively, and then stored at 5
. Keeping qualities of the products were investigated by measuring moisture content, H, volatile basic nitrogen (VBN), viable cell count, thiobarbituric acid (TBA) value, peroxide value (POV) and color value. Good qualities of the products stored at 5
were maintained at least 9 days, 11 days, more than 35 days and 24 days in air, vacuum, CO2 gas and N2 gas packaging products, respectively. In cases of 2
storage, however, the shelf-lives of all the products were only one day. The shelf-life extension of the products were observed in CO2 ga>N2 gas>vacuum>air packaging in order.
Properties of the Chemical Composition of Safflower (Carthamus tinctorius L.) sprout
Journal of Life Science, volume 10, issue 1, 2000, Pages 68~73
In order to develop new materials for the functional food, the components of safflower(Carthamus tinctorius L.) sprout was studied. Chemical composition, minerals, amino acids, and fatty acids of the leaf and the stem parts of 2 weeks grown safflower sprout were analyzed. The chemical composition of safflower spout was 12.7% (w/w) moisture, 28.5% crude protein, 10.1% crude fat, and 5.3% crude ash. Mineral contents of the leaf part were P 49%, K 22%, Mg 15%, Ca 10% whereas these of the stem part were K 51%, P 27%, S 10%, Ca 7%, Mg 4%. Other mineral contents were less than 2% in both parts. Especially, Pt was 0.18 ppb and 0.17 ppb in the leaf and the stem parts, respectively. The composition of the amino acid were approximately as follow, the major amino acid in the leaf and the stem were aspartic acid and glutamic acid, the contents of these were 10.7mg/g, 10mg/g in the leaf, 11.3 mg/g, 8.4 mg/g in the stem, respectively. The major fatty acids in the leaf and the stem parts were linoleic acid and
-linolenic acid. The linoleic acid (C18:2) in the leaf and the stem parts were 67% (w/w) and 47% whereas the
-linolenic acid. The linoleic acid (C18:2) in those parts were 14% (w/w) and 11%, respectively. On the basis of chemical analysis, the safflower sprout showed to have relatively high contents of crude protein and crude fat, minerals including small amount of Pt, polyunsaturated fatty acid as linoleic acid and
-linolenic acid. These results suggested that safflower sprout was found to be a useful material of natural health food for the functional food development.
Airborne Microorganisms of Some Underground Shops in Pusan
Journal of Life Science, volume 10, issue 1, 2000, Pages 74~78
This study was investigated to evaluate the distribution of airborne microorganisms at underground shops in Seomyun and Nampodong, Pusan. The number of bacterial colonies on the nutrient agar plate plates were calculated by the open petri dish method for 30 minutes in indoor air of underground shops at every seasons in a year. There was no statistically significant difference between Seomyun and Nampodong in mean colony counts. Isolation rates of Gram positive rods was highest, and Gram positive cocci and Gram negative rods were followed. In Nampodong underground shops, Enterobacteriaceae strains was isolated. Mean colony counts according to seasons was higher at summer and autumn in Seomyun, and spring and winter in Nampodong. In near future, a study on the distribution of bacteria causing respiratory infection should be followed.
The Effects on Quinone ReductaseInduction of Daucus carota L.
Journal of Life Science, volume 10, issue 1, 2000, Pages 79~85
Chemoprevention is one of the major strategies for cancer control. It is well established that dietary factors play an important role in modulating the development of certain types of human cancer. The experiment was conducted to determine quinone reductase(QR) activity induction of Daucus carota L. on HepG2 cells. Among various partition layers of roots of Daucus carota L., the ethyl acetate partition layer(DCMEA) and the n-hexane partition layer(DCMH) tested to be most effective which resulted 2.1 and 1.6 respectively compared to the control value of 1.0. In the case of seeds of Daucus carota L. n-butanol partition layer (DCMB) on HepG2 cells at a dose of 200
showed the highest induction activity of QR which was 3.0. These results suggest that potentially useful cancer chemoprevention chemicals could be isolated from DCMEA and DCMH of the roots and DCMB of the seeds of Daucus carota L.
Structure and Hydrolysis Study of Inclusion Complex of Cyclodextrin and Aspirin
Journal of Life Science, volume 10, issue 1, 2000, Pages 86~93
Specific molecular recognition of cyclodextrin and aspirin was determined. A stable 1:1 inclusion complex was established in solution. The distinct structure of inclusion complex was elucidated by FT-IR, FAB-MS, UV, 1H NMR, and 13C NMR spectroscopy. Based on the 1H NMR data, a time-averaged conformation of
-cyclodextrin exhibited significant catalytic activity toward the hydrolysis of aspirin in alkaline solution.
Isolation of Urease Positive Vibrio parahaemolyticus and Urease Production
Journal of Life Science, volume 10, issue 1, 2000, Pages 94~100
Urease is an important microbial enzyme and its production is a marker to predict potential pathogenicity. An unusual halophilic bacterium producing urease was isolated from sea product and identified as Vibrio parahaemolyticus KH410. Its biochemical properties were indole negative, gelatin positive, sodium citrate positive and Kanagawa positive whereas other characteristics were identical as the standard strain except it showed a positive reaction on Christensen's urea agar. V. parahaemolyticus urease production was directly related to urea concentration. The production of urease was noticeable by the addition of 0.2% urea, 0.5% glucose, 2% NaCl in LB broth, and the initial pH of 5.5. The maximum production reached after 6 hr of incubation at 37
. However, NiCl2, metal ions, phosphorus did not affect production of urease.
Isolation and Characterization of Pectinase-Producing Bacillus sp. BS-214
Journal of Life Science, volume 10, issue 1, 2000, Pages 101~106
A bacterial strain BS-214 producing extracellular pectinase was isolated from soil. The isolated bacterium was identified as a strain of Bacillus so. based on the morphological, biochemical, and physiological characteristics. Cell growth and pectinase activity of Bacillus sp. BS-214 were reached to a mixium in the culture condition of pH 8.5 at 4
. Production of pectinase by the strain was the highest when polygalacturonic acid was added to culture medium as a carbon source, and its optimal concentration was 1%. Also, yeast extract was used as the best nitrogen source for the production of pectinase by the concentration of 0.25%. Decomposition of a constituent of Edzeworthia papyrifera by the strain was observed by scanning electron microscope.
Comparision of Peroxidase, Superoxide dismutase and Diterpene cyclase Activity under Environmental Stress Conditions in Rice
Journal of Life Science, volume 10, issue 1, 2000, Pages 107~113
This study carried out to investigate activities of the superoxide dismutase(SOD), peroxidase(POD) and diterpene cyclase of rice plant treated by environmental stress conditions. The rice leaves of Ilpum was shown to have the most highest specific activity of POD. POD activity in the susceptible cv. Ilpum and Hwasung during the disease progress show a increase, while for a decreasing activity in the resistant cv. Dasan. SOD activity of rice plants treated by chitosan were increased as the treatment concentration increased. At the chitosan application, the SOD activity in the susceptible cv. Hwansung at the rice leaves 14days after treatment was higher in treatment than chitosan-untreated control. The activity of POD in the susceptible cv. Ilpum decreased as long as UV-B irradiation, while that in the resistant cv. Dasan increased. Futhermore, activity of that in Dasan were similar to as time on UV irradiation, Hwasung showed two times higher than untreated control at 40 minutes after UV-B exposure. The activity of diterpene cyclase in rice plants inoculated with Pyricularia oryzae has much higher tendency than UV-B irradiation. The activity of diterpene cyclase in the rice leaves gradually increased until 5 days after inoculation with Pyricularia oryzae.