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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 11, Issue 6 - Dec 2001
Volume 11, Issue 5 - Oct 2001
Volume 11, Issue 4 - Aug 2001
Volume 11, Issue 3 - Jun 2001
Volume 11, Issue 2 - Apr 2001
Volume 11, Issue 1 - Feb 2001
Selecting the target year
Study on Acute Subcutaneous Toxicity of Hydroxyapatite Sinter Produced from Tuna Bone in Sprague-Dawley Rats
Journal of Life Science, volume 11, issue 2, 2001, Pages 97~102
This study was performed to evaluate the actue sbucutaneous toxicity of hydroxyapatite sinter produced from tuna bone in Sprague-Dawley(SD) rats. Hydroxyapatite sinter was administrated at dose levels of 5000, 2500, 1250, 625, 312.5 and 0 mg/kg. After single subcutaneous adiminstration to both sexes to both sexes SD rats, we observed rats for 14 days. Hydroxyapatite sinter did not induce any toxic signs inmortalities, clinical findings, body weights and gross findings of the rats. In view of result, it was impossible to estimate LD/ sub 50/ values in SD rats. In conclusion, these results suggest that hydroxyapatite sinter produced from tuna bone has no effect on acute subcutaneous toxicity in SD rats.
Effects of Silkworm(Bombyx mori L.) Power on Oxidative Stress and Membrane Fluidity in Brain of SD Rats
Journal of Life Science, volume 11, issue 2, 2001, Pages 103~110
This study was designed to investigate the effects of silkworm(Bombyx moril L.) powder on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley(SD) male rats(160
10 g) were fed basic diet(control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400mg/kg BW/day for 6 weeks. There were no significant differences in cholesterol levels of brain memberanes by administration of silkworm powder (SWP). Membrane fluidities were significantly increased(21.5% and 30.8%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but significant difference between brain microsomes could not obtained. Basal oxygen radicals (BORs) in brain mitochondria and mircrosomes were significantly inhibited(8.5% and 16.5%, 16.8%and 24.8%, respectively) by SWP-200 and SEP-400 groups compared with control group. Induced oxygen radicals(IORs) in brain mitochondria were significantly inhibited(16.6% and 21.4%, respectively)by sWP-200 and SWP-400 groups compared with control group, but IOR in brain microsome were significantly inhibited about 16.0% by SWP-400 groups only compared with control group. Lipid peroxide(LPO) levels were significantly decreaed(14.8%and 22.4%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but LPO level was significantly decreased about 16.0% in brain microsome of SWP-400 group only. Oxidized protein(OP) levels were remarkably decreased(about 14.8% and 16.5%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups, but OP level was significantly decreased about 13.0% in brain microsome of SWP-400 group only compared with control group, Theses results suggest that administration of in brain microsome of SWP-400 group only compared with control group. These results suggest that administration of SWP may play effective role in attenuating an oxidative stress and increasing a membrane fluidity in brain membranes.
Histological effects of ricin on liver, spleen, thymus, lung and heart of mouse
Journal of Life Science, volume 11, issue 2, 2001, Pages 111~120
The pathological aspects of purified ricin from the seeds of the castor oil plant, Ricinus communis, were examined, using light and transmission electron microscopy. ICR mice were exposed to ricin by peritoneal injection with 100 ng/1
PBS(pH 7.0) mouse and histological observations on the liver, spleen, thymus, lung and heart were carried out at intervals up to 48 h after exposures. All the organs examined were damaged by ricin. Among the organs, the spleen and thymus; immune organs were the most sensitive to ricin, whereas the effect delayed in the liver, lung and heart. Furthermore, the immune cells in each organ were the most sensitive to ricin. Accordingly, the effect of ricin on the organs seems to be affected by the immune cells existed in each organ, In each organ, the immune cells showed apoptotic changes, while the capillary endothelial and parenchymal cells showed necrotic changes.
Differences in Nymphal Development, Adult Longevity and Fecundity of the Green Rice Leafhopper, Nepthotettix cincticeps Uhler Fed on Rice Cultivar and Water Foxtail, Alopecurus aequalis Sobol. Var. amurensis Ohwi at Various Temperatures
Journal of Life Science, volume 11, issue 2, 2001, Pages 121~125
Some attempts were made to investigate the biological characterisitcs of the green rice leafhopper(GLH) , Nepthotettix cincticeps Uhler in terms of nymphal development, emergence ratio. preovipositional period, growth index, adult longevity and fecundity fed on rice cultivar, Chucheongbyeo with no resistance gene and water foxtail, Alpecurus aequalis Sobol. var. amurensis Ohwi at 20 and
with a 16L;8D photoperiod. Development period of nymph on rice cultivar and water foxtail at
was shorter than that at 2
by twofold. The nymphal period of female was about one day longer than that of male. The mean emergence ratio of female and male on rice cultivar and water foxtail was 40.0, 76.7, 38.3, 73.3% at 20 and
, respectively. Preovipositional period on rice cultivar and water foxtail at
was shorter that at 2
by twofold. Growth index on rice cultivar adn water foxtail at
was higher than that at 2
by fourfold. Under the 20 and
condition, the longevity of female and male was longer on water foxtail than on rice cultivar, but the fecundity of GLH was higher on rice cultivar than on water foxtail. In conculsion, water foxtail seems to be adequate for spring host of GLH.
Effect of Quartz Porphyry on the Functional and Morphological Changes of Liver and Kideny in Common Finch and white Java Sparrow
Journal of Life Science, volume 11, issue 2, 2001, Pages 126~132
Effect of Quartz porphyty(QP) on functional and morphological changes of the liver and kidney was studied in male common finch and white java sparrow fed with the basal diet(Control group) or experimental diet containing 3.0% QP(QP group) for 14 days. There was not significantly different morphological change of the liver upon light microscopic examination in common finch and white java sparrow between control group and QP group. Morphological change of renal tissue upon light microscopic examination in common finch and white java sparrow was not also significantly different between control group and QP group. The concentrations of serum creatinine, blood urea nitrogen, and nric acid as renal functional parameters of common finch and white java sparrow were not significantly different in the both groups. The activity of glutamic oxaloacetic transaminase(GOT) as hepatic functional parameter in common finch was significantly higher in the QP group(
<0.05), whereas the activity of glutamic pyruvic transaminase(GPT) as hepatic functional parameter in common finch was not significantly different in the both groups. The activities of GOP and GPT in white java sparrow were not significantly different in the both groups. The morphologic findings and functional parameters of the liver and kidney observed in common finch and white java sparrow fed with 3.0% QP diet showed evidence of slightly liver damage accompanied with increased release of enzyme and fatty change of the hepatocytes in common finch, suggested that the tissues in some animals can be damaged by feeding a diet supplemented with 3.0% QP.
Are the genus Moniliella and Trichosporonoides closely related in molecular taxonomic relationship\ulcorner
Journal of Life Science, volume 11, issue 2, 2001, Pages 133~137
The molecular taxonomic relationship of nine species in the genus Moniliella Stolk & Dakin and Trichosporonoides Haskins & Spencer and six species of other yea나-like fungi was examined by sequencing analysis of large subunit rDNA D1/D2 variable domain. The fifteeen species fell into two major groups corresponding with their genetic relationships. The nine species of the genus Moniliella and Trichosporonoides were placed at the same cluster. similarity values based on the D1/D2 domain sequences were 45.4-100% among species of genus Moniliella, 45.2-84.4% among genus Trichosporonoides species, and 45.6-90.1% among species of genus Moniliella and Trichosporonoides. Identical sequence similarity was observed between M. suaveolens var. nigra and M. suaveolens. A colse relationship of M. mellis. and M. acetoabutens is observed. The result of this study provided and insight into the genetic origins of genus Moniliella and Trichosporonoides species as well as their genetic relationships. Genus Moniliella and Trichosporonoides are closely related to each other based on sequence analysis of the large subunit rDNA D1/D2 region and we suggest combination of the genus Moniliella and Trichosporonoides to single genus.
Processing of Corbicula elatior Beverage
Journal of Life Science, volume 11, issue 2, 2001, Pages 138~143
Marsh calm(Corbicular elatior)with a short-term storage in raw and a law-rate of utilization has been increasing the needs to develop new marsh calm processing products for a temporary mass treatment and long-term distribution, Therefore the processing conditions of marsh calm beverage using proteolytic enzyme hydrolysis were investigated. A partial hydrolysis at 6
for 1 hour after adding 3% Alcalase as more effective than a hot water extraction to develop taste compounds from the marsh calm. The result of ommission test showed that nucleotides and their related compounds were contributed in the taste of the marsh calm hydrolysates rather than free amino acids. The taste of the hydrolysates was produced by association with these compounds rather than only one compound s the hydrolystes taste differently for the control when one of these compound was omitted. The hydrolysates were fractionated to molecular weight below 500 dalton to eliminate bitter taste and to improve it flavor from the hydrolysates, 0.05% bay leaf was more effective to improve the odor than other herbs.
Changes of Chemical Components in Kochujang Added Sea Tangle Powder During Fermentation
Journal of Life Science, volume 11, issue 2, 2001, Pages 144~152
In order to improve functionality of kochujang which is one of the traditional foods of Korea, sea tangle powder(2, 4, 6 and 8% sea tangle powder on the glutinous rice weight basis) was added to the raw material of kochujang and then investigated the change of physiochemical properties with control kochujang during the fermentation at 3
for 120 days. During 120 days fermentation, moisture and crude protein contents were gradually decreased with fermentation time, whereas crude fat content NaCl were slightly increased with fermentation time. The contents of reducing sugar of sea tangle kochujang was rapidly increased until 60 days of fermentation after that it was increased slightly up to 90 days of fermentation to the highest value and then reduced slightly or not changed approximately until 120 days. pH was reduced up to 60 days of fermentation after that it remained 4.63~4.91 in 90~120 days. Acidity was increased with fermentation time, and it was the highest value of 11.5~12.4
in 120 days of fermentation. Viscosity was increased with increased with fermentation time. Especially a case of additional sample of 8% sea tangle powder was highly increased until 60 days. A case of color difference value, in initial time of preparation of kochujang was distinct difference of value for additional samples respectively but as the fermentation progressed, among the samples not found consistent change of color difference value. L value was gradually decreased during fermentation. A value was decreased up to 30 days of fermentation and it was increased again totally at 60 days of fermentation. B value was decreased totally at initial time of fermentation and a case of control was increased again at 90 days of fermentation, the other samples increased again 60 days earlier than 30 days.
Effects of Soil Acidification on Growth of Impatiens balsamina L. and Tagetes patula L. Plants
Journal of Life Science, volume 11, issue 2, 2001, Pages 153~158
To investigate the effects of soil acidification on growth of Impatiens balsamina L. plants were transplanted to acidified soils with H
solution. The concentrations of soluble Ca, Mg, K, Al and Mn in the acidified soils increased with increment of H
addition to the soil. In both species, the plant height and root length were inhibited by soil acidification, showing much severer inhibition in Impatiens balsamina L. than in Tagetes patula L., As the soil pH decreases, the growth of underground parts decreased greatly than that of above ground parts in both species. Total dry weight decreased with increased Al concentration as well as lowered soil pH in both plants. There was a strong positive correlation between relative total dry weight and molar (Ca+Mg+K) / Al ratio of the soil. The results suggest that molar(Ca+Mg+K)/ Al ratio of the soil may be useful indicator for assessing the critical load of acid deposition in herb species.s.
Ear and Kernel Characteristics of Korean Indigenous Maize Lines Collected in Pusan and Kyungnam
Journal of Life Science, volume 11, issue 2, 2001, Pages 159~165
In order to reserve abundant germplasm for breeding new corn varieties, major characteristics of ears and kernels were evaluated with a total of 210 Korean indigenous maize lines collected from various parts of Pusan City and Kynugnam Province, Korea The average ear length and ear diameter of indigenous maize lines collected was 12.52cm and 3.33 cm, respectively. The average ear weight of the maize lines was 63.70g. The ears collected from the north-west mountainous region were the heaviest, and The ears from the south coastal region were the lightest. The average kernel weight per ear was 50.54g, and the kernel weight per ear by region showed a tendency similar to the ear weight. The kernels of maize lines collected in the north-west mountainous region were the longest, and kernel width and thickness were the largest in the west plain region. 100 kernel weight and embryo weight the largest in the lines collected in the north-west mountainous region. The degree of pericarp thickness was the smallest in the lines collected in the south coastal region, and largest in the lines collected in the west plain region. Except for the correlation coefficient width and 100 kernel weight, all correlation coefficients between the characteristics of the lines showed highly significant differences.
Effect of Blue, Red and Far-red Lights on Seeding Growth and Cotyledon Chlorophyll Content of Lagenaria siceraria Standl
Journal of Life Science, volume 11, issue 2, 2001, Pages 166~172
Various types of the seeding of bottle gourd widely used as a rootstock of watermelon has been required to satisfy the farmers need. The study was done to determine the effect of light quality of blue, red and far-red lights treated with light emitting diodes on growth and morphology of bottle goured seeding and chlorophyll content of its cotyledons. The lights were treated in the growth chamber for 7 days to the seeding elapsed 8 days after sowing under natural condition, and 64 hole trays with commercial bedsoil. Plant height, length and diameter of hypocotyl, leaf area of cotyledon and first true leaf, its leaf length, number of true leaves, fraction and total dry weight were measured. Red light shortened and slenderized the hypocotyl, which lengthened by far-red light and thickened by blue light. Plant height was declined in order of far-red light treatment, blue and red lights mainly due to difference of hypocotyl length, Area and length of the first true leaf became smaller and shorter under far-red light than under the other lights. However, blue light increased leaf area of cotyledons. Two cultivars cv. Yongjadaemok and cv. Kunghap had different response to the light treatments in total seedling dry weight(dw); far-red and red light treatments showed the greatest and the least dw of hypocotyl, respectively, while blue and red lights did the greatest dw of the other organs. Among the ratio of each organ dw to total dw, those of hypocotyl and true leaves were different between the light treatments; the highest ratio of hypocotyl dw to total dw was observed in far-red light treatment but the lowest was in red light treatment. Those of the true leaves were the lowest in far-red light and similar response in blue or red light treatment. Chlorophyll content of cotyledons was decreased in order of red light treatment, blue and far-red lights, meaning that short period light treatment may influence photosynthesis of seeding and afterward its growth.
Cultural Characteristics of Antagonistic Bacterium, Bacillus licheniformis N1 against Botrytis cinerea
Journal of Life Science, volume 11, issue 2, 2001, Pages 173~180
This study was conducted to estimate the cultural characteristics, the production of antibiotic, and the selection of optimal media for mass culture of Bacillus licheniformis N1 isolate which was previously reported as an antagonistic bacterium to Botrytis cinerea. We investigated initial pH, temperatures and shaking speed for good cultural conditions and antibiotics production by N1 isolate. According to the results, the optimal conditions of initial pH, temperatures, and shaking speed were determined to be pH 5.0~5.5, 30~35
and 250 rpm, respectively. Also, the optimal conditions for the antagonism by N1 isolate highly appeared in the initial pH as 5.0, and the mycelial growth inhibition was high when the substances used such as glucose or corn starch as carbon sources, and biji(soybean curd residue) flour as a nitrogen source. Furthermore, inhibitory area was significantly expanded, when 3% or 5% of corn starch was added into 5% of Biji flour as nitrogen source, were respectivley selected for mass culture of N1 isolate. Among them, 5% Biji flour medium showed higher cell density more than 10 times that in NB medium after 48 hour incubation. Therefore, the optimal medium was determined as 5% biji flour added 3~5% of corn starch for high density of cells.
Effects of Helicobacter pylori Antigen on Producton of Transforming growth factor-
1 and Nitric oxide in Human Fibroblast
Journal of Life Science, volume 11, issue 2, 2001, Pages 181~189
Cytokines are hormone-like proteins which mediate and regulast inflammatory and immune responses. Transforming growth factor -
) plays an important role in the control of the immune response and wound healing, and in the development o various tissues and organs, Nitric oxide(NO) is major messenger molecule regulating immune function and blood vessel dilation and serving as a neurotransmitter in the brain and peripheral nervous system. Also, NO is to be a potent mutagen that cause mutation in the p53 tumor suppressor gene in early phases of human gastric carcinogenesis. The purpose of this study was to investigate the effect of Helicobacter phlori lystes, lipopolysaccharide (LPS), and Staphylococcus enterotoxin B(SEB) on production of TGF-
1 and NO by human fibroblasts. Primary cultured human fibroblasts were incubated with H. pylori lysates(Hp), LPs, SEB, Hp+LPS, Hp+SEB, Hp+LPS+SEB. Cultured supernatants that were collected at 24, 48 and 72 hr were assessed for TGF-
1 by enzyme-linked immunosorbent assay and NO production by quantification of nitrite ion. TGF-
1 production in fibroblasts exposed with Hp, LPS or SEB for 48 hrs was enhanced, but for 72 hrs inhibited. Its production by doble exposure such as Hp+LPS, Hp+SEB, Hp+LPS+SEB was lowered in comparison with single exposure of Hp in cases of 24 and 48 hrs incubation, but for 72 hrs decreased in Hp vaculoating toxin(+), increased in Hp vacuolating toxin(-). No production in fibroblasts increaed at all doses of LPS. But its production by exposure of SEB increased or decreased according to dose and incubation time. Also, NO production by Hp vacuolating toxin(+) increased at all doses, but its production by Hp vacuolating toxin(-) decreased. Its production by doble exposure such as Hp+LPS, Hp+SEB, Hp+LPS+SEB decreased in comparison with single exposure Hp Therefore, quantities pf TGB-
1 and NO released by human fibroblasts shows differences according to kinds of stimulants. Also, in care stimulated with same kinds of stimulants, its productions exhibit quantitative differences according to exposure times. These results suggest that the decreased of TGF-
1 in fibroblasts by mixed exposure with Hp producing vacuolating toxin and bacterial toxins such as LPS and SEB may effect negatively in healing of host tissue and increased of NO by infection oh H. pylori may related to the increased susceptibility for human gastric carcinogenesis.
Expression Characteristics of Recombinant Cyclodextrin Glucanotransferase in Saccharomyces cerevisiae
Journal of Life Science, volume 11, issue 2, 2001, Pages 190~195
The cuclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subcloned at the downstream of yeast ADH1 promoter, and then the resulting plasmid pVT-CGTM(9.15 kb) was introduced into the yeast host strain, Saccharomyces cerevisias 2805. The transformed yeast, S. cerevisiae 2805/pVT-CGTM, showed the starch-hydrolyzing activity on the starch-azure plate. The optimal conditions for the CGTase expression were found to be 2% dextrose, initial pH5.5, 3
, and 48hr cultivation. Under this condition, the extracellular CGTase activity reached at 0.53 U/mL, whereas the intracellular activity was about 0.03U/mL. This result indicates that the signal peptide of Bacillus CGTase functioned well in S. cerevisiae.