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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 11, Issue 6 - Dec 2001
Volume 11, Issue 5 - Oct 2001
Volume 11, Issue 4 - Aug 2001
Volume 11, Issue 3 - Jun 2001
Volume 11, Issue 2 - Apr 2001
Volume 11, Issue 1 - Feb 2001
Selecting the target year
Changes of Enzyme Activities in Kochujang Added Sea Tangle Powder During Fermentation
Journal of Life Science, volume 11, issue 5, 2001, Pages 393~399
In order to improve functionality of kochujang which is one of the traditional foods in Korea, sea tangle powder(2, 4, 6 and 8% sea tangle powder on the glutinous rice weight basis) was added to the raw material of kochujang and then investigated the bacterial counts and enzyme activities with control kochujang during the fermentation at 3
for 120 days. Bacterial count was about 10
cfu/g at initial stage of fermentation and then maintained 10
cfu/g after 60 days of fermentation.
-amylase activity was gradually reduced during fermentation periods, so the activity was lost almost at late of fermentation
-amylase activity was rapid increased until 30 days of fermentation and the rapid decreased at 60 days of fermentation and after 90 days was slightly decreased. Activities of acidic protease and neutral protease were increased until 30 days of fermentation and then these were shown irregularities decreased.
Identification and Phylogeny of Long Terminal Repeat Elements of Human Endogenous Retrovirus HERV-S
Journal of Life Science, volume 11, issue 5, 2001, Pages 400~404
A new human endogenous retroviral family (HERV-S) has recently been identified from human X chromosome. It is 6.7 kb in length and has a typical retroviral structure with LTR-gag-pol-env-LTR. Using the PCR and sequencing approach, we investigated LTR elements of the HERV-S family from a human genomic DNA. Four LTR elements (HSL-1, HSL-5, HSL-10, HSL-11) were identified and have a high degree of sequence similarity(96-99%) with that of the HERV-S. Phylogenetic analysis from the HERV-S family indicated that the LTR elements were mainly divided into 2- groups through evolutionary divergence in the primate evolution. Further investigation of the HERV-S LTR elements in primates may cast light on the integration timing into the primate genome and understanding of human evolution.
Influence of Protaetia brevitarsis Extract on Liver Damage Induced by Carbon Tetrachloride and Ethanol in Rats
Journal of Life Science, volume 11, issue 5, 2001, Pages 405~414
Protaetia brevitarsis has been utilized as an ingredient of the description for the treatment of patients with chronic hepatic diseases in oriental medicine. This study was attempted to investigate whether Protaetia brevitarsis extract(PBE) protects or modulates the liver injuries induced by carbon tetrachloride or ethanol in Sprageue-Dawley rate. The liver injuries of rats induced by the treatment of carbon tetrachloride or ethanol were manifested by the observation of the significant changes in liver weight, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, serum thiobarbituric acid reactive substances (TBARS), and microsomal detocification enzymes(cytochrome P_450), cytochrome b
, and cytochrome b
reductase).The effect of PBF on the liver damage induced by the chemicals was evaluated with the extent modulated in change of biochemical parameters above. Exposure to ethanol alone resulted a significant change in the ration of liver per body weight, ALT activity, and microsomal detoxification enzymes (cytochrome P_450, cytochrome b
, and cytochrome b
reductase), but did not significantly changes in the levels of serum AST activity and TBARS. Pretreatment coith PBE did not modulate the alteration of the ratio of liver to body weigth, and the activities of serum aminotransferascs (AST. ALT), TBARS, and micro somal detoxification enzyme (cytochrome p_450, cytochrome b
,and cytochrome b
reductase. These result suggested that PBE has not appreciable therapeutic effect on carbon tetrachloride or ethanol induced hepatotoxicity.
Molecular Characterization of a Bombyx mori Protein Disulfide Isomerase(bPDI)
Journal of Life Science, volume 11, issue 5, 2001, Pages 415~422
Many secreted proteins have disulfide bonds that are important for their structure and function. Protein disulfide isomerase (PDI, EC 188.8.131.52.), an enzyme that catalyzes the formation and rearrangement of thiol/disulfide exchange reactions, is a resident of the endoplasmic reticulum (ER). The subcellular localization and its function as catalyst of disulfide bond formation in the biosynthesis of secretory and cell membrane proteins suggest that PDI plays a key role in the secretory pathway. We have isolated a cDNA encoding protein disulfide isomerase from Bombyx mori(bPDI). It has been characterized under ER stress conditions (dominantly induced by calcium ionophore A23187, tunicamycin and DTT), which is known to cause an accumulation of unfolded proteins in the ER. Furthermore, It has also been examined for tissue distribution(pronounced at the fat body), hormonal regulation (juvenile hormone, insulin and juvenile +transferrin; however, it is not effected by transferrin alone), and the effect of exogenous bacteria (peak at 16 h after infection) on the bPDI mRNA expression. The results suggest that bPDI is a member of the ER stress protein group, and it may play an important role in exogenous bacterial infection in fat body, and that homones regulate its expression.
Molecular cloning of the Arabidopsis gene rat3 that is involving in the Agobacterium-mediated planttransformation
Journal of Life Science, volume 11, issue 5, 2001, Pages 423~431
Genomic and cDNA clones containing the RAT3 gene involving in Agobacterium-mediated plant transformation were identified using plant DNA flanking the righ border of a T-DNA rescued from the rat3 mutant as hy-bridization probe. Two highly homologous cDNA clones were identified; one (RAT3-1) weakly hybridized with the probe whereas another (RAT3-2) strongly hybridized with the probe. Both Rat3-1 and Rat3-2 proteins contain a putative signal peptide for secretion. The deduced molecular weights of encoded proteins are 15 kDa. The results of genomic DNA blot analysis and DNA sequencing indicated that RAT3-1 and RAT3-2 exist as single copy genes and they were arranged side by side with just 600 bp distance between them. RAT3-1 was disrupted by the integration of T-DNA into the 3 untranslated region in rat3 mutant. A BLAST search showed that both RAT3-1 and RAT3-2 proteins have homology with only the C-terminal region of
-1,3-glucanase homologues from Triticum aestivum and Arabidopsis thaliana. Thses
-1,3-glucanase homologues contain an unusually long C-terminal region with no sig-nificant homology to other
Isolation and Characterization of Lactic Acid Bacteria Producing Antimutagenic Substance from Korean Dongchimi
Journal of Life Science, volume 11, issue 5, 2001, Pages 432~438
Various lactic acid bacteria were isolated from Korean Dongchimi (whole radish Kimichi with added water) in order to study their antimutagenic activity. Ames test using Salmonella enterica serovar typhimurium TA98 and TA100 showed the strain DLAB19 to have the highest antimutagenic activity among the 300 isolated strains against MNNG(N-methyl-N-nitro-N-nitrosoguanidine), NPD (4-nitro-O-phenylenediamine), 4-NQO(4-nitroquinoline-1-oxide) and AFB
). The strain was identified as Leuconostoc mesenteroides subsp. cremoris according to the Bergeys Mannual Systematic Bscteriology based on its morphological, cultural, physiological characteristics and biological system Antimutagenic activity of Leu. mesenteroides subsp. cremoris DLAB19 was found in the culture supernatant suggesting the bacterium secretes, the antimutagenic substance in the media. The antimutagenic activity of Leu. mesenteroides subsp. cremoris DLAB19 was reconfirmed by the spore-rec assay using spores of Bacillus subtilis H17 (Rec
) and M45 (Rec
Antimutagenic Effects against N-methyl-N`-nitro-N-nitrosoguandine and 4-nitroquinoline-1-oxide on Cultrue Conditions of Leuconostoc mesenteroides subsp. cremoris DLAB19 isolated from Dongchimi
Rhee, Chang-Ho ; Joo, Gil-Jae ; Woo, Cheol-Joo ;
Journal of Life Science, volume 11, issue 5, 2001, Pages 439~446
Leuconostoc mesenteroides subsp. cremoris DLAB19 were investgated under various culture conditions to maximize the production of antimutagenic substance(s) against N-methyl-N\`-nitro-N-nitrosoguandine(MNNG) on Salmonella enterica serovar typhimurium TA100 and 4-nitroquinoline-1-oxide(4-NQO) on S. enterica serovar typhimurium TA98. The MRS medium containing glucose (2%) as a carbon source and yeasty extract (1%) as a nitrogen source resulted in the highest production of the antimutagenic substance(s) against both mutagens in the culture supernatant of Leu. mesenteroides subsp. cremoris DLAB19. Optimal pH of the culture medium, culture temperature and shaking speed for the antimutagenic substance(s) production were pH 7.0, 3
and 150 rpm, respectively. Under the optimal condition, the antimutagenic effects of Leu. mesenteroides subsp. cremoris DLAB19 culture supernatant were 96.4% against MNNG on S.enterica serovar typhimurium TA100 and 53.8% against 4-NQO on S. enterica serovar typhimurium TA98.
Development Toxicity Evaluation
Journal of Life Science, volume 11, issue 5, 2001, Pages 447~456
We have recently demonstrated that the fluoroquinolone antibacterial DW-116 caused a significant developmental toxicity in rats. The present study was conducted to determine whether the development toxicity induced by DW-116 treatment was the result of malnutrition fro reduced food intake or the direct effects of test chemical on conceptuses. The test chemical was administered by gavage to pregnant rats from gestational days 6 through 16 at dose levels of 0 and 500 mg/kg/day. A pair-feeding study was also performed in which the pregnant rats received the same amount of diet consumed by the DW-116-treated pregnant rats. All dams were subjected to caesarean section on day 20 of gestation and their fetuses were examined for examined for external, visceral, and skeletal abnormalities. In this treatment group, the maternal toxicities included increased abnormal clinical signs, decreased maternal body weight, suppressed body weight gain during treatment and posttreatment periods, and reduced food intake. The significant developmental toxicities included increased fetal deaths, decreased live fetuses, reduced fetal body weight and placental weight, increased incidence of fetal abnormalities, and increased fetal ossification delay. In this pair-fed group, however, slight maternal toxicities including decreased body weight and suppressed body weight gain during treatment period were observed in comparison with the control group, and minimal development toxicities including reduced fetal and placental weights and increased fetal ossification delay were found. The number of fetal deaths and live fetuses, and the incidences of malformed fetuses and litters with affected fetuses were comparable to the control values. Based on the results, it could be concluded that the development toxicity observed in the treatment group is attributable to the direct effects of Dw-116 treatment, but not to the maternal malnutrition from reduced food consumption during pregnancy.
Purification and Characterization of Extracellular Lipase from Staphylococcus xylosus SC-22
Journal of Life Science, volume 11, issue 5, 2001, Pages 457~463
A bacterial strain SC-22 which produced alkaline lipase was isolated from salf-fermented shrimps. Strains SC-22 was identified as Staphylococcus xylosus. An alkaline lipase excreted by Staphylococcus xylosus SC-22 was purified by ammonium sulfate predipitation and column chromatography on Sephadex G-100 and DEAE-Sephace. The specific activity of purified lipase was 756U/mg of protein with 17.2% yield. The approximate molecular weight of the purified enzyme was 47 kDa. The partially purified lipase preparation had and optimum temperature of 4
, an optimum pH of 8.0, and a stable of 5~10. Lipase activities were enhanced by salt ions such as
while inhibited remarkably by heavy metal ions, C
Cloning and Characterization of dnaK Heat Shock Protein Gene in a Halotolerant Cyanobacterium
Journal of Life Science, volume 11, issue 5, 2001, Pages 464~469
A gene, dnaK2, encoding a distinct member of the HSP70 family of molecular chaperones is isolated from the halotolerant cyanobactrium Aphanothece halophytica. The dnak2 gene encodes a molecular wight of 68 kDa polypeptide with predicted 616 amino acid residues. The DnaK2 protein has a structural characteristic of bacterial DnaK homologues and shows high similarity to other HSP70/Dank proteins. The danK2 transcripts are hardly detectable at 28
and strongly induced upon heat stress. It is also found that dnaK2 transcript is increased by high-salinity stress even in the absence of heat stress. These results suggest that the DnaK2 protein plays an important role in protecting A. halophytica against damage caused by salt stress at well as heat stress.
Naupia Development of Nitocra spinipes Boeck, 1865 (Copepoda, Harpacticoida, Ameiridae) Reared in the Laboratory
Journal of Life Science, volume 11, issue 5, 2001, Pages 470~475
The larvae of Nitocra spinipes Boeck, 1865 were reared at 2
in the laboratory. These species passed through six nauplii stages and the first copepodid appeared in 8-10 days. Nauplii of N. spinipes is distinguished by characteristics of the appendage setation and caudal setae. The number of segments of antennal and mandibular exopodites in harpacticoida is discussed within familial level.
Effect of Sea Tangle, Laminaria japonicus, Extract on The Activities of Glucokinase and Hexokinase in Alloxan-Induced Diabetic Mellitus Mice
Journal of Life Science, volume 11, issue 5, 2001, Pages 476~482
The study was undertaken to determine the effect of the aqeuous extract of sea tangle, Laminaria japonicus, on the activites of glucokinase and hexokinase in the pancreas of diabetic mellitus mice which were induced by alloxan. After one week of alloxan injection, the levels of serum glucose and insulin secretion were dramatically increased, however, the insulin secretion was decreased with administration of sea tangle extract. Alloxan injection allowed the serum glucose level to increase and level was decreased by sea tangle extract administration. Furthermore, it was observed the sea tangle extract was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase were decreased by alloxan treatment. In contrast, sea tangle extract administration to the mice allowed to increase proportionally. These results suggested that sea tangle extract is highly effective in treatment of diabetic mellitus induced by alloxan.
Physico-Chemical Properties and Antimicrobial Activity of Pyocyanine Produced by Pseudomonase aeruginosa KLP-2
Journal of Life Science, volume 11, issue 5, 2001, Pages 483~488
The antimicrobial substance produced by Pseudomonas aeruginosa KLP-2 strain was purified and identified. The substance was identified as a pyocyanine by the fast atom bombardment mass(FAB-MS). In physic-chemical properties, the pyocyanine was dark blue needles, and was soluble in various organic solvents such as chlorogorm, methanol, ethanol and ethyl acetae. The pyocyanine possessed a ultraviolet absorbance spectrum in methanol, 0.1 M HCl, and chlorogorm. The maximum absorption peak of the pyocyanine showed at 318 mm in methanol. The molecular formula of the pyocyanine was determined to the
O and protonate molecular ion species (M+H)
was observed at m/z 211 by FAB-MS. The pyocyanine showed antimicrobial against Bacillus cereus, Bacillus subtilis, Micrococcus luteus, Rodococcus equi, Staphylococcus aureus, Streptococcus faecalis, E. col, Legionella pneumophila, Shigella flexneri Shigella boydii, shgella sonnei, NAG Vibrio cholerae, Vibrio parahaemolyticus, Vibro vulnificus, Yersinia enterocolitica, and Saccharomyces cerevisiae. However, Salmonella spp. Shigela dysenteriae, 3 strains of Pseudomonas aeruginosa, Klebsiela pneumoniae, and Aspergillus niger were resistant to the pyocyanine. The pyocyanine showed the highest antimicrobial activity aganist Legionella pneumophila based on the size of inhibition zone by the disk contained 0.5
of the pyocyanine.e.
Ozone-water Treatment on the Morphological Changes of Endosperm cell and the activity of Acid Phosphatase during Soybean(Glycine max) Germination
Journal of Life Science, volume 11, issue 5, 2001, Pages 489~495
The effect of ozone-water treatment on the morphological change of endosperm cells and the activity of acid phosphatase during Glycine max germination was investigated with electron microscope. Acid phosphatase showed the activity in the cell organelles of germinating endosperm of seed. it's activity occurrs in 12 hrs cultivation after 0.5 ppm ozone-water treatment. As the differentiation of endosperm, reaction products of the acid phosphatase appear to be accumulated invacuole after treatment of ozone-water. This result confirm that acid phosphatase is inveolved in the decomposition and translation of the intracellular storage materials. The characteristics of grganelle in the endosperm cell during germination were discussed.
A STUDY ON THE MYCELIA CUL TURE AND GENETIC CHARACTERISTICS IN Lepista nuda
Journal of Life Science, volume 11, issue 5, 2001, Pages 496~501
This study was carried out to investigate the cultural characteristics and the genetic characteristics in Lepists nuda. The mycelia cultural characteristics which include the specificity for pH, carbon source, nitrogen source and medium were studied using petridish culture and liquid media culture respectively. The genetic characteristics were also investigated using random amplified polymorphic DNA (RAPD) analysis. To find out the mycelial growth rate by various medium, mycelia was cultured for 25 days in PDA, YM, MEA, GPB and Yamanaka. The Yamanaka was superior to the other media in supporting the mycelial growth. pH6 produced the best result in the test of an optimal pH. In experiment for optimal carbon source, starch showa 46.8
1.7mm of diameter of mycelial colony as the best and for nitrogen source, yeast extract showed a good effect as well. In investigation for genetic characteristics, Lepists nuda was amplified by 7 primers among 10 primers and Pleurotus ostreatus was also amplified by 7 primers. From the RAPD analysis between species, the band patterns were different.