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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 11, Issue 6 - Dec 2001
Volume 11, Issue 5 - Oct 2001
Volume 11, Issue 4 - Aug 2001
Volume 11, Issue 3 - Jun 2001
Volume 11, Issue 2 - Apr 2001
Volume 11, Issue 1 - Feb 2001
Selecting the target year
Antioxdative Effect of the Essential Oil form the Rhizomes of Acorus gramineus
Journal of Life Science, volume 11, issue 6, 2001, Pages 503~508
The essential oil of Acorus gramineus(Aracease), which has been used as an antioconvulsant in Korean folk medicine, was evaluated for its effects on antioxidative system in vitro and in vivo. This mixture of terpenes showed inhibitory effects on xanthine oxidase activity with 13.3% at 10
/ml and on aldehyde oxidase activity with 5.0% at 1
/ml. Lipid perosidation was inhibited by 49.4% at 1.0 mg/ml of the essential oil in vitro and by 16.7% after 7 days inhablation of an oil as compared to PTZ-treated control group. DPPH radical scavenging activity of this essential oil was ralatively werak
Evaluation of the Antioxidant Potential and ldentification of Active Principles of Solanum nigrum L. on Antioxidant Defense Systems
Journal of Life Science, volume 11, issue 6, 2001, Pages 509~516
Enzymes and non-enzymatic antioxidants are involved in defense of oxgen free radical intermediates in all aerobic cells. The non -enzymatic antioxidants and antioxidant enzyme from the extracts of Solanum nigrum L. known to be anticancer medicinal plant were examined in other to utilize the discovery in natural products as cancer chem-opereventive agents. The DPPH(1,1-diphenyl-2-picryl-hydrazyl) free radical scavening activity on plant position of Solanum nigrum L. was the highest in root, with stem, whole plant, seed, leaf and flower, at higher activities respectively. In extraction methods, the DPPH free radical scavenging activity by circulating extraction with 80 % MeOH. The DPPH activity of L6 fraction by LH-20 column chromatography showed about 6.7 times higher than that of ethyl acetate-fraction. These were identified as phenolic compounds such as 2-6-methano-3-benzazocin-11-ol, 2[1H]-phyidinethione and 2-hydroxy -5-methyl-benzaldehyde. Peroxidase(POD) and superoxide dismutase(SOD) activities of stem and root were higher than that of other plant positions and those of plant positions according to growing stage were the highest in 60 days after seeding. The numbers of isozyme pattern of POD and SOD showed 10 hands and 5 bands, respectively, especially, 8 bands of POD and 3 bands of SOC showed a difference according to plant positions.
Taste Compounds of Small Black Bean Chungkugjang Added with Kiwi and Radish
Journal of Life Science, volume 11, issue 6, 2001, Pages 517~522
To Confirm the repression of off-odor and improvement of food quality in small black bean chungkugjang (SBC), some taste compounds of SBC added with kiwi and radish homogenate and fermented fro 3 days at 42
were investigated. Contents of free amino acids in SBC were lower than that of soybean chungkugjang (SC) and were increased by adding kiwi and radish homogenate to black bean, indicating that two materials were effective to the enzymatic digestibility of soy protein. Of organic acids, citric acid was the most abundant, followed by acetic acid and lactic acid. Fatty acid composition was high in the order of linoleic acid (50.82~54.51%), oleic acid (17.76%~22.10%) and plamitic acid(12.13~13.79%). There were no significant difference in compositions of organic acids and fatty acids of chungkugjang. Major volatile compounds of SBC were indole , 2-5-dimethyl pyrazine and trimethyl pyrazine. Contents of alkyl pyrazines that contribute the characteristic aroma and flavor of SBC added and fermented with kiwi and radish homogenate were 3 times lower than those of SC. Uracil and UMP were major nucleic acie-drlated compounds in all four chungkugjangs. Contents of the other nucleic acid-related compounds were a similar trend in all chungkugjangs.
Foraging activities by bumblebee, Bombus terrestris S. (Hymenoptera: Apidae) at two cultivating types of cherry-tomato house
Journal of Life Science, volume 11, issue 6, 2001, Pages 523~529
The foraging activities of bumblebee, Bombus terrestris were surveyed to compare with two types of cherry-tomato (Lycopersicon esculentum. M) house as Nutriculture and Sawdustculture on southern part of Korea in winter . The summary of the result obtained for this purpose are below: In the interval of blooming time from Sawdustculture type. In the interval of the time from the first visting on the flower by a worker of B. terrestris after a flower of cherry-tomato bloomed. nutriculture type was more shorter than Sawdustculture type. Therefore there was no difference in the average pollination time from the flower's blossoming according to the inflorescence of the first visiting time of B. terrestris worker on the each flower between Nutriculture type and Sawdustculture type. The foraging activites of B. terrestris worker in two kinds of cherry-tomatio cultivating house had shown same patterns. and han not been found to the differences.
Isolation of Oil-Degrading Bacterium, Providencia rettagei 4A3 and Characterization of Crude Oil Degradation
Journal of Life Science, volume 11, issue 6, 2001, Pages 530~536
Several bacterial strains utilizing crude oil as their sole carbon and energy source were isolated from marine environment polluted by crude oil. Among them, the selected strain 4A3 showed strong degradation activity for crude oil. This strain was identified as a Provindencia rettgeri 4A3 based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions was as follows; 26
for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 2.0%, indicating that this strain was derived from sea water. The emulsifying activity of 4A3 was the highest after 3 days of cultivation under the condition of 2.0% sodium chloride, pH 7.0 and 26
. This strain had one cryptic plasmid in 7.0kb size.
-Aminobutyric acid Concentrations in Korean Plants and Mushrooms
Journal of Life Science, volume 11, issue 6, 2001, Pages 537~542
The concentrations of
-aminobutyric acid in some Korean plants and mushrooms were investigated.
-amino-butyric acid concentrations in Morus alba leaves, Cudrania tricuspidate leaves and fruits were 134.03, 104.13 and 120.99 mg/100g dry matter basis, respectively,
-aminobutyric acid concentrations in Pleurotus ostreatus, Flammulina velutipes and Auricularia auricula were 93.44, 72.17 and 66.48 mg/100g dry matter basis. respectivley.
Curative and Protective Effects of Garlic on Lead Poisoning
Journal of Life Science, volume 11, issue 6, 2001, Pages 543~553
Lead poisoning is currently one of the major public health problems in industrialized countries. Several chelating agents have been developed and used to treat the lead poisoning. It was recently proposed that garlic, having a large quantity of S-S compounds, may react readily with lead, producing various sulfur componds, and thus provide curative and protective effects on the lead toxicity. The present study was undertaken to evaluate this possibility in experimental animals. The garlic treatment on rats pre-exposed to lead significantly enhanced urinary excretion of lead at a garlic dose of 500 mg/kg and decreased the bloon level of lead at both 250 and 500 mg/kg doses. The administration of grilic 500mg/kg along with lead significantly alleviated the renal histological alterations induced by lead. These results suggest that garilic has a curative as well as a protective effect against lead poisoning , thus it may act as a chelator of lead.
Antibacterial Activity of Poncirus trifoliata Juice against Pathogenic Bacteria
Journal of Life Science, volume 11, issue 6, 2001, Pages 554~560
For development of functional food, antibacterial effect of Poncirus trifoliata juice was examined. Strong antibacterial activities of Poncirus trifoliata juice were observed aginst Gram positive and negative pathogenic bacteria such as Baillus cereus, Bacillus subtilis, Corynebacterium zerosis, Listeria monocytogenes, Micrococcus luteus, Rhodococcus equi, Klebsiella pneumoniae, Pseudomonas aeruginosa, Vibrio alginolyticus, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vnlnificus and Yersinia enterocolitica. The minimum inhibitory concentration(MIC) of Poncirus trifoliata juice against Bacillus cereus. Listeria monocytogenes, Micrococcus luteus, Rhodococcus equi, Staphylococcus epidermidis, Citrobacter freundil and Pseudomonas aeruginosa was 2.5% and the MIC against Vibrio cholerae, Vibrio parahemolyticus, Vibrio vulnificus and Yersinia enterocolitica was 1.25%. Also, antibacterial activities of Poncirus trifoliata juice treated for 15 min at 121
were confirmed to be stable.
Isolation and Purification of Fibrinolytic Enzyme of Edible Mushroom, Sarcodon aspratus(Berk.)S. Ito
Journal of Life Science, volume 11, issue 6, 2001, Pages 561~567
To isolate and purify fibrinolytic active substance from Sarcodon aspratus(N
precipitation, DE52 anion exchange column chromatography, Sephacryl-S 200gel filtration chromatography and Mono S cation FPLC were carried out and the characterizations of the purified enzyme were investigated. The bound active fraction on DE52 anion exchange column chromatography were eluted with 0.2 M NaCI and the fibrionlytic enzyme was purified after following Sephacryl-S200 gel fitration chromatography and Mono S cation EPLC. The specific activity of purified enzyme was 55.2 U/mg protein and increased 11.3 fold comparing crude extract and the yield was 49.5%. 12% SDS-PAGE electrophoresis and gel filtration chromatography revealed that Sarcodon aspratus fibrionloytic enzyme was highly purified and had 29.300 Da molecular weight. Enzyme activity of the purified fibrinolytic enzyme from Sarcodon aspratus was increased on higher pH and was stable until pH 10.5. On temperature dependent stability, the enzyme activity was decrease sharply but remained 25% relative activity on 8
. This enzyme activity was inhibited by heavy metal ion, C
with 68% and 38%, respectively. And also, the enzyme activity was inhibited with
chelator EDTA and serine protease inhibitor PMSF. These results from this study suggested that the fibrinolycit enzyme from Sarcodon aspratus is a serine protease and the enzyme activity was increased by
Effect of Alkali Treatments on the Greenness and Volatile flavors of Sea Lettuce, Monostrima nitidum
Journal of Life Science, volume 11, issue 6, 2001, Pages 568~573
Sea lettuce(Monostrima nitidum) were treated with several alkali agents on condition with water blanching at 9
for 10 min., and followed by drying and powdering, thus, the power samples treated and the not treated were then allowed to be stored at
, dark place for 5 months. The sea lettuce powder samples were evaluated for green color intensity and volatile flavor at a month intervals. The green intensity of the samples were measured by using Hunter-lab colorimeter, therefore, the decoloration of greenness were seen in both of the samples treated and the not treated, but there were slight inhibitory effects on decoloration in the treated with alkali agents, especially in the treated with KHCO
.The volatile flavor of the samples were collected by simultaneous distillation-extraction, and then the 31 flavor compounds were separated on HP-5 capillary column(25m
0.25mm i.d) and identified by using GC-MS. From these results, it was presumed that the characteristic impact flavor compounds were
-ionone. The total content of the characteristic impact flavor compounds decreased in the samples treated with alkali agents more alkali agents more than in the not treated, but lee decrease was observed in the treated with KHCO
Activity of Superoxide dismutase(SOD) by fermented soybean
Journal of Life Science, volume 11, issue 6, 2001, Pages 574~581
This study was performed to evaluate the inhibition effects of fermented soybean on lipid perosidation and antioxidative relative enzyme activity. in vivo. Fermented soybean was induced the high SOD activity, while significantly inhibited on the peroxide value of linoleic acid and lipid perxidation from rat microsome induced by Fe
ascorbate system, Sprague-Dawley(SD) male rats were fed basic diet, and experimental diets group added 200 or 500 mg/kg fermented soybean for 2 weeks. The effect of fermented soybean is also significantly increased catalase and glutathione peroxidase activities, while significantly inhibited the lipid peroxidation of rat liver microsome in a dose dependent manner. Therefore, these results suggest that fermented soybean has antioxidative activity which is related enzyme to prevention of oxidative stress.s.
Effects of Sulfur Dioxide Exposure in Histological Structure and Mucosubstances of the Nasal Respiratory Mucosa of Rat
Journal of Life Science, volume 11, issue 6, 2001, Pages 582~594
This experiment was performed to investigate the effects of sulfur dioxide on the histological changes, properties of mucosubstances and glycoconjugates of the nasal respiratory mucosa in the rat. Sprague-Dawley male rats weighing about 200~250g were divided into a control group and SO
exposed groups. Again SO
exposed groups were divided into 10 ppm, 25 ppm, 50 ppm, 100 ppm, and 200 ppm subgroups, according to concentrations of SO
and each SO
exposed groups were divided into 1, 3 and 6 hours groups. For the histological changes, hematoxylin-eosin(H-E) and periodic acid Schiff's(PAS) stainings were used, and for the properties of mucosubstances, PAS, alcian blue (AB) pH 2.5, pH 2.5-PAS, AB pH 1.0 and aldehyde fuchsin (AF) pH1.7-AB pH 2.5 were used. In all the SO
exposed groups, loss of cilia and detachment of epithelial cells, vacuolation of goblet cells were observed in the respiratory epithelium while epithelial squamous metaplasia and intraepthelial mucous cells were observed in the higher concentration of SO
and the degree of the loss cilia was higher according as concentration was higher and exposed time was longer. The intraepitheial mucous cells appeared most remarkable in the 50 ppm SO
exposed group. The numbers of goblet cells and acini of nasal septal gland were varied according to concentration of SO
and exposed time, but the numbers in the 25 ppm and 50 ppm, SO
exposed increased remarkably. However, the numbers in the 100 ppm and 200 ppm SO
exposed group had a tendency to decrease noticeably, or disappeared.
Histochemical Study of Musocubstances in Esophageal Mucous Cells of Sebastes schlegli, Halichoeres poecilopterus, Bryzoichtys lysimus and Takifugu pardalis
Journal of Life Science, volume 11, issue 6, 2001, Pages 595~602
The prelectin histochemical methods had been applied to study mucosubstances properties of esophageal mucous cells in four teleostean species, i, e.. sebastes schlegli, Halichoeres poecilopterus, Bryzoichtys lysimus and Takifugu pardalis. The following methods were used: periodic acid Scheff"s(PAS) reaction, alcian blue(AD) pH 2.5, AB pH 1.0, AB pH 2.5-PAS, aldehyde fuchsin (AF) pH 1.7-AB pH 2.5 and high iron diamine (HID)-AD pH 2.5 stainings. The number size and shape of esophasgeal mucous cells studied depend on the fish species. Esophageal mucous cells of Sebastes schlegli and Halichoeres poecilopterus were mixed with large, medium sized and small mucous cells, but these cells of the species were mixed with medium sized and small mucous cells. The large esophageal with moderate to considerable amount of acid mucin. Most of the large mucous cells in these species contained neutral mucin and strongly sulfomucin, whereas a few mucous cells contained neutral mucin, strongly sulfomucin, and sialomucin. Medium sized and small mucous cells of these species contained considerable to large amount of neutral mucin, and small to considerable amount of acid mucin, Most of the medium sized and small mucous cells contained neutral mucin and sialomucin, but a few mucous cells contained neutral mucin and strongly sulfomucin or neutral combined with strongly sulfomucin and sialomucin. Most of the esophageal mucous cells pf Bryzoichthys lysimus contained small amount of neutral mucin, while on the other hand a feww mucous cells contained small amount of neutral mucin and minimal amount of sialomucin. But the esophageal mucous cells of Takifugu pardalis contained considerable amount of neutral mucin only.
The Effect of Environmental Factors on the Production of Phytoalexin in Papper plant(Capsicum annumm L.)
Journal of Life Science, volume 11, issue 6, 2001, Pages 603~611
Phytolalexins are produced in plants affected by various environmental factors such as fungal infection treatment with many chemicals and irradiation by ultraviolet light. When pepper and tobacco bel suspension cultures were grown on a basal MS medium supplemented with 2,4-D(1mg/
, benzyl adenine(0.001 mg/
) and 100
M jasmonic acid, the production of capsidiol was observed. The total of compound found in pepper plant were around seventy and thirty of them were located intissue-specific manner. 1-propanethiol,
-D-xylofuranoside, phenol, hexadecanonic acid ethyl tridecanoate, phytol, linoleic acid and capsidiol are those which have change the production level by treatments, such as the inoculation of Phytophthora capsici Leonian, the metalaxyl treatment and the UV-B irradiation, respectively. The content of capsidiol on inoculation of P. capsici with metalxyl suspension in soil were higher than those of P.capsici without metalaxyl. When the soil dernch of metalaxyl treatment (1
)was delayed after inoculation, the content of capsidiol were higher than that of before. Irrradiated UB-B the production on capsidiol was identified only at leaf, and contents were the highest for 24 hrs incubation after 20 minutes irradiation.
-Radiation on Growth and Antioxidant Enzyme Activities in Red Pepper
Journal of Life Science, volume 11, issue 6, 2001, Pages 612~617
Seedlings of red pepper (Capsicum annum L.) were exposed to various doses of
Co)and cultivated in the 1/2,000 a pot. Plant hight the number of leaves, flowers and fruits, chlorophyll contents, plant dry weight, ac- tivites of antiozidant enzymes such as superoxide dismutase(SOD) adn peroxidase(POD), were determined. Plant height of seedling treated with 1 Gy of
-radiation was increased up to 16.6% as compared to untreated control at 80 days after treatment, while those treated with 20 Gy or 50 Gy was decreased 22.0% or 75.0% respectively. The plants treated with 1 Gy or 5 Gy were increased the number of leaves up to 41.2% and 24.9% as well as dry weight of fruit up to 58.4% and 49.4% respectively. However treatment of radiation higher than 20 Gy significantly inhibited the growth, chlorophyll content and yield of pepper fruit. Activities of antioxidant enzymes, SOD and POD were tem-porary increased within one or five days after treatment depending on the doses of treatment , and the activities were gradually decreased to control level of lower thereafter. The SOD and POD activities in the leaves of pepper plant treated with 50 Gy were 5.5 and 6.0folds higher than that of control at five days after treatment, respectively.