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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 12, Issue 6 - Dec 2002
Volume 12, Issue 5 - Oct 2002
Volume 12, Issue 4 - Aug 2002
Volume 12, Issue 3 - Jun 2002
Volume 12, Issue 2 - Apr 2002
Volume 12, Issue 1 - Feb 2002
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The Effect of the Membrane Fluidity of Bellflower(Platycodon grandiflorum A.) Fractions on Liposomal Phospholipid Membranes
Journal of Life Science, volume 12, issue 2, 2002, Pages 121~128
DOI : 10.5352/JLS.2002.12.2.121
The object of this study was to investigate the effect of membrane fluidity of bellflower(Platycodon grandiflorum A. DC, ; PG) fractions in phosphatidylcholine(PC) liposomes, measured with high-sensitivity differential scanning calorimetry(DSC). We used dipalmitoylphosphatidylcholine(DPPC) bilayers which slake most stable liposomes among the other phosphatidylcholine. The sample PG was extracted and fractionated to five different types : butanol(PGMB), ethylacetate(PGMEA), ethylether(PGMEE), hexane (PGMH) and methanol(PGMM). Among five different solvent fractions, the PGMEE, PGMEA, PGMH and PGMM fractions markedly affected the thermotropic properties of DPPC liposomes, broadened and shifted the thermograms, and reduced the cooperative unit. It might be said that the incorporation of PGMEE, PGMEA and PGMH in DPPC liposomes were located in the hydrophobic core of DPPC bilayers and, PGMM and PGMB in the hydrophilic core of DPPC bilayers. These results suggest that certain substances in the PGMEE, PGMEA and PGMH fractions might have biologically significant effect on the membrane fluidity.
Effect on Livestock Manure Composting by the Enriched Microbial Population
Journal of Life Science, volume 12, issue 2, 2002, Pages 129~135
DOI : 10.5352/JLS.2002.12.2.129
Several kinds of thermophilic, aerobic microorganisms (Bacillus genus), metal leaching microorganisms (Thiobacillus, T. ferooxidans), and other nondegradable chemical-degrading microorganisms (Pseudomonas genus) were utilized to study the effect on composting livestock manure. Under the Carbon-Nitrogen ratio (C/N) of 35∼40 and water content of 50∼65% conditions, the composting in the cycling drum reactor showed slower composting and lower temperature increase than that of the manual reactor. Element analysis after composting indicated relatively high levels of mineral contents with the substitutional effect of chemical fertilizer. Metal analysis before and after composting showed lower As in all, Cr in pig, Pb in cow, Hg in chicken, and Cu in cow manure compost than the regulation values. Compost maturity was ascertained by the several maturity tests. Salmonella and E. cozi detection test by SS or EMB agar plate confirmed the safety from the pathogenic microorganisms. The results suggest that the inoculation of metal and some other chemical degrading microorganisms during composting might decrease metal contamination and increase composting rate.
Protective Effect of Jaboyangyeong-hwan Water Extracts on CCl
-Induced Liver Damage
Journal of Life Science, volume 12, issue 2, 2002, Pages 136~143
DOI : 10.5352/JLS.2002.12.2.136
Jaboyangyeong-hwan (IAE) has been known as a traditional medicine for the treatment of debility, fatigue, and liver diseases. The hepatoprotective effect of the water extract of Jaboyangyeong-hwan was investigated against carbon tetrachloride (
)-induced hepatic damage. A single intraperitoneal injection of
produced liver damage in rats as manifested by the significant rise of aspartate aminotransferase (AST, alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in serum as compared to those of untreated normal group. Pretreatments of rats with the JAE extract (300, 600, and 1200 mg/kg for 7 days) were significantly reduced AST, ALT, and ALP levels compared with
-treated control group. Treatment of rats with
led to significantly increase in lipid peroxidation and significantly decrease in cytochrome P450 and P450 reductase. The oral administration of the JAE extract significantly inhibited the accumulation of microsomal thiobarbituric acid reactive substance (TBARS) and increased the cytochrome P450 and P450 reductase activity. All these biochemical alterations resulting from
administration were inhibited by the pretreatment with JAE extract. These results suggest that JAE water extract can be useful as a hepatoprotective agent.
Antioxidant Activities of Chrysanthemum coronarium L. Fractions on the Liposomal Phospholipid Membrane
Bae, Song-Ja ; Noh, Ok-Jeong ;
Journal of Life Science, volume 12, issue 2, 2002, Pages 144~150
DOI : 10.5352/JLS.2002.12.2.144
This experiment was designed to investigate the antioxidant effects of Chrysanthemum coronarium L. (CC) fractions on the liposomal phospholipid membranes. The sample CC was extracted and fractionated into five different types, methanol (CCMM), hexane (CCMH), ethylacetate (CCMEA), butanol (CCMB), and aqueous (CCMA) fractions. The antioxidant activities of CC fractions in oxidized dilinoleoylphosphatidylcholine (DLPC) liposomes were examined by spectrophotometry measuring conjugated dienes. The oxidation indices of five CC fractions exhibited weaker antioxidant activities than that of BHT in oxidized DLPC liposomes, however, showing much similar antioxidant activities of
-tocopherol in the oxidized DLPC liposomes, which is known as a potent antioxidant. Among CC fractions, CCMM and CCMA in oxidized DLPC liposomes showed rather effective than
-tocopherol after 2 h. These results strongly indicate that bioactive substances in CC fractions have a kind of function as potent antioxidants against biomembrane oxidation.
Purification and Characterization of Trypsin Inhibitor from Alismatis Rhizoma
Journal of Life Science, volume 12, issue 2, 2002, Pages 151~157
DOI : 10.5352/JLS.2002.12.2.151
A trypsin inhibitor was isolated and purified from Azismatis Rhizoma which has been used as a galenic for diuretic and antiphlogistic. Purification was carried out by 0-80% saturated ammonium sulfate salting out, DEAE- cellulose ion exchange chromatogrphy, Sephadex G-150 gel filtration. The molecular weight of Alismatis Rhizoma trypsin inhibitor(ARTI) was estimated to be about 23,000 Da by gel filtration and SDS-PAGE, it must be monomer. ARTI was stable at 0~6
, but at higher temperature its activity was decreased about 35%. When benzoyl-dl-arginine p-nitroanilide was used as a substrate of trypsin, half-maximal inhibition of ARTI was observed at 0.071
M. ARTI inhibited the hydrolysis of trypsin non-competitively and Km value was 0.81
Axenic Isolation and 16S rRNA Gene Sequence of the Cyanobacterium Microcystis aeruginosa in Downstream of Nakdong River
Journal of Life Science, volume 12, issue 2, 2002, Pages 158~163
DOI : 10.5352/JLS.2002.12.2.158
For axenic isolation of the cyanobacterium Microcystis aeruginosa, water bloom at the Mulgum station from the Nakdong River was pretreated by shaking with distilled water. Removal of bacteria was accomplished using antibiotics (150
ampicillin and 25
neomycin) and colonizing on CB solid medium prepared from 0.7% agarose at 3
light. Among 26 strains of the Microcystis species, only three strains were axenically established. The three strains were examined by PCR-amplified 16S rRNA gene and 16S rRNA sequencing. The similarities were 99.5 ~100% with M. aeruginosa AF 139292.
Polyamines Modulate Growth factor-Induced Membrane Protein Phosphorylation in MCF-7 Human Breast Cancer Cells
Lee, Ji-Young ; Kim, Ji-Hyun ; Lee, Kyeong-Hee ; Kim, Byeong-Gee ;
Journal of Life Science, volume 12, issue 2, 2002, Pages 164~172
DOI : 10.5352/JLS.2002.12.2.164
Breast cancer cell lines display a wide variety of growth factor receptors, and considerable evidences implicate the importance of signalings from those receptors. A useful prognostic indicator would be the level of activity of a second messenger protein used in common by these receptors. Our studies were designed to obtain preliminary information on the possible role of polyamine as a mediator of the membrane-associated protein phosphorylation and as a regulator of second messenger in mitogenic signal of estrogen or growth factors in MCF-7 human breast lancer cells. DFMO significantly inhibited the phosphorylation induced by
and EGF in membrane-associated proteins (154, 134, 116, and 104 kDa). Exogenous polyamines abolished the inhibitory effect of DFMO. Tyrosine phosphorylations of membrane-associated proteins were not increased by
or growth factor treatments and not affected DFMO treatment. Polyamine administration markedly enhanced the tyrosine phosphorylation of membrane-associated proteins (154, 134, and 116 kDa). In the present study,
and EGF enhanced protein phosphorylation in the almost same levels. These data indicate that
and growth factor signaling pathway may cross-talk through various protein kinase which phosphorylated many substrate proteins (154, 134, 116 and 104 kDa). Polyamines may be involved in growth signaling pathway of
or EGF for the cross-talk through regulation of the protein phosphorylation such as 154, 134, 116 and 104 kDa. Polyamine may also selectively interfere with several different protein kinases, and the specific steps in signal transduction system were effected by polyamines.
Studies on the Comparative Analysis of Mating Locus (Y-region) of Schizophyllum commune
Journal of Life Science, volume 12, issue 2, 2002, Pages 173~181
DOI : 10.5352/JLS.2002.12.2.173
This study was conducted to do the comparative analysis of mating type locus controlling the direct formation of fruiting body in Schizophyllum commune which is indigenous to North America with that of other identified mating locus. The 3120 bp Y-region nucleotide of A
3 mating locus activating a developmental pathway in S. commune was determined, and appeared to have about 96% homology to S. commune 1-71
3 allele indigenous to South America, showing strongly a conservative feature. This nucleotide analysis also showed above 96% homology highly in the seven presumed exons, and about 97% in the acidic rich region (AR), about 99% in homeodomain (H7), about 97% in the basic rich region (BR), about 95% in the serine rich region (Ser) respectively. In the comparative analysis to the translated polypeptide sequence, S. commune A
3 mating locus containing Y-region also showed about 97% homology to the region of S. commune indigenous to North America, but the identity ratio to Y1 including Y4, Y5, Y6 different allele types was declined to about 41~49%. In the analysis of functional loci controlling mating activity, it is assumed to have a highly conservative feature showing about 98% homology in homeodomain polypeptide. Especially, it is notable that the homology ratio of above 85% in homeodomain motif between mating type alleles was higher than in the AR, BR, Ser showing about 10~50% homology.
Extracts of Caesalpina sappan L. Potentiate the Apoptosis of NIH3T3 Cells Exposed to Methymethane Sulfonate
Journal of Life Science, volume 12, issue 2, 2002, Pages 182~187
DOI : 10.5352/JLS.2002.12.2.182
In this study we have investigated the effect of Caesalpina sappann L. extracts on the apoptosis in NIH3T3 cells exposed to methylmethan sulfonate (MMS), an alkylating agent. MTT assay study showed that Caesalpina sappan L. extracts potentiate the MMS-induced viability. Cell morphology studies, acridine orange (AO) staining, and DNA fragmentation analysis indicated that the postincubation of Caesalpina sappan L. extracts increase the nuclear condensation of MMS-induced apoptotosis. These results suggest that Caesalpina sampan L. extracts contain components potentiating MMS-induced apoptosis of NIH3T3 cells.
Molecular Cloning and Analysis of Nucleotide Sequence of Xylanase Gene (xynk) from Bacillus pumilus TX703
Journal of Life Science, volume 12, issue 2, 2002, Pages 188~199
DOI : 10.5352/JLS.2002.12.2.188
A gene coding for xylanase from thermo-tolerant Bacillus pumilus TX703 was cloned into Escherichia coli DH5
using pUC19. Among 7,400 transformants, four transformants showed clear zones on the detection agar plates containing oat-spells xylan. One of them which showed highest xylanase activity was selected and its recombinant plasmid, named pXES106, was found to carry 2.24 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynK) was determined, xynK gene was found to consist of 1,227 base-pair open reading frame coding for a polypeptide of 409 amino acids with a deduced molecular weight of 48 kDa. The coding sequence was preceded by a putative ribosome binding site, the transcription initiation signals, and cia-acting catabolite responsive element. The deduced amino acids sequence of xylanase is similar to those of the xylanases from Hordeum vulgare (barley) and Clostridium thermocellum, with 39 and 31% identical residues, respectively. The amino acids sequence of this xylanase was quite different from those of the xylanases from other Bacillus species.
Isolation and Antifungar Activity of Bacillus ehimensis YJ-37 as Antagonistic against Vegetables Damping-off Fungi
Journal of Life Science, volume 12, issue 2, 2002, Pages 200~207
DOI : 10.5352/JLS.2002.12.2.200
This study was carried out to isolate of antagonistic bacterium against Pythium ultimum and Rhizoctonia solani AG-4, causal pathogens of vegetables damping-off. Total of 600 strains were isolated from soil and plait roots. The isolates were screened for antagonism against Pythium ultimum and Rhizoctonia solani AG-4. One strain, named YJ-37, was sellected for detained study among those microoganisms screened. It was identified as Bacillus ehimensis based on morphological and physiological characterisitics according to the Bergey`s mannual of systematic bacteriology, Sherlock system of Microbial ID Inc. and 16S rDNA sequences methods. Furthermore Bacillus ehimensis YJ-37 showed antifungal activities against Alternaria altrata, Collectotrichum gloeosporioides, Didymella bryoniae, Fusarium moniliforme, Fusarium oxysporum, F. oxysporum cucumerinum, F. oxysporum niveum, Gloeosporium sp., Glomerella sp., G. cingulata, G. lagenaria, Penicillium digitatum, P. italicum, Phytophthora capsici, Sclerotinia sclerotiorum, and Stemprhylium solani.
Effect of Neighbor Base Sequences on the Base Pair Stabilities at d(CXG) and d(GXC) in Human ε-globin Promoter
Chung, In-Ae ; Gang, Jong-Back ;
Journal of Life Science, volume 12, issue 2, 2002, Pages 208~212
DOI : 10.5352/JLS.2002.12.2.208
-globin DNA fragment was used to determine the thermal stabilities of base pairs at d(CXG) and d(GXC) by Temperature Gradient Gel Electrophoresis(TGGE). The base pair stability depends on the hydrogen bonding interaction and base stacking interaction of neighbor base sequence. The orders of base pair stabilities were T.A
Genetic Analysis of Quantitative Characters by Diallel Cross in Cotton
Journal of Life Science, volume 12, issue 2, 2002, Pages 213~218
DOI : 10.5352/JLS.2002.12.2.213
The experiment was conducted to get basic information for cotton breeding program through four-variety diallel cross population. Additive, dominant, maternal, and reciprocal effect were observed significantly for days to maturity. Based on the Vr-Wr graphical analysis, the characters, number of bolls per plant, boll weight, seed and lint weight per boll, lint weight per boll, width of seed, 100-seed weight, days to flowering, and days to maturity were found to inherit incomplete dominance, and the characters of number of bolls per plant, width of seed, number of seeds per boll, and length of seed were assumed to inherit over dominance. The component of genetic variance analysis for boll weight, seed and lint weight per boll, lint weight per boll, number of seeds per boll, length of seed, and days to flowering showed that additive effects were higher than dominant effects. The narrow-sense heritability(h
N) for boll weight, seed and lint weight per boll, lint weight per boll, days to flowering, and days to maturity showed high values as more than 43.19%. The estimate of broad-sense heritability(h
B) value was higher than that of h
N because of the low importance of dominance effects.
Characterization of a New Gene Resistant to Alkylating Agents and 3-Aminobenzamide When Knocked Out in Fission Yeast
Journal of Life Science, volume 12, issue 2, 2002, Pages 219~225
DOI : 10.5352/JLS.2002.12.2.219
The organization of eukayotic chromatin into specific conformation that are associated with transcription, replication, reapir and other nuclear processes are achieved via a series of DNA-protein interaction. These interactions are mediated by a range of DNA-binding domains such as SAP domain et at. By searching S. pombe genomic DNA database, we have found a gene named SAPuvs (SAP UV Sensitive) whose amino acid sequence is in part similar to SAP domain of Arabidopsis poly (ADP-ribose) polymerase and Ku7O. Knock-out cell of S. pombe SAPuvs gene was constructed using Ura4 as a selection marker. Survival analysis of knock-out cell indicated that treatment with UV significantly reduces the survival compared to wild type cell. Potentiation of MMS-induced cytotoxicity by 3AB post-treatment was observed in wild type cells, but not in knock-out cells. These data suggested that the protein encoded by SAPuvs gene is associated with chromatin reorganization during DNA repair.
A Study on the Levels of Leptin, Insulin and Serum Lipids of Primary School Boys Depending on %Fat
Journal of Life Science, volume 12, issue 2, 2002, Pages 226~232
DOI : 10.5352/JLS.2002.12.2.226
In this study, the researcher classified primary school boys with obesity into light(% fat : 20∼24.9%), moderate(25∼29.9%) and heavy(30% or more) obese group and then analyzed and evaluated their own levels of insulin, leptin and lipids in blood. The level of insulin was significantly high in primary school students with heavy obese rather than in the other two groups. While that of leptin, significantly high in those children with moderate and heavy obese rather than in the other group of light obese. The level of T-C was significantly high in primary school students with light obese rather than in those students with heavy obese. HDL-C tended to increase as %fat went up, but there was no significant difference. The level of TG was significantly high in the group of heavy obese compared to the other groups. LDL-C tended to increase gradually as %fat became high. Thus, depending on %fat, primary school students with obesity were significantly different with each other in insulin, leptin T-C and TG levels. This suggests the urgent necessity of developing systematic and separate programs to treat such obese children.