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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 12, Issue 6 - Dec 2002
Volume 12, Issue 5 - Oct 2002
Volume 12, Issue 4 - Aug 2002
Volume 12, Issue 3 - Jun 2002
Volume 12, Issue 2 - Apr 2002
Volume 12, Issue 1 - Feb 2002
Selecting the target year
Effects of exercise on sleep EEG following caffeine administration
Journal of Life Science, volume 12, issue 4, 2002, Pages 375~382
DOI : 10.5352/JLS.2002.12.4.375
The purpose of this experiment was to examine influence of acute exercise on nocturnal sleep which had been disrupted by caffeine(400mg
3) thought the daytime. Six healthy young males aged 21.0
0.2 yr with a history of low caffeine use. Subjects completed three conditions in a within-subject. At three conditions Sleep EEG were investigated: (1) nocturnal following quiet rest, (2) nocturnal sleep following the consumption of 1200mg of caffeine (3) nocturnal sleep following cycling at 60 min of 60% V
with 1200mg of caffeine consumption. Sleep data were calculated for REM sleep, REM latency, sleep onset latency, sleep efficiency, sleep stages, SWS. Those data were analyzed using repeated-measures ANOVA of change scores. A main effect to, drug(caffeine) indicated that caffeine elicited sleep disturbance that is, TST and sleep onset latency increase and sleep efficiency and stage 4 decrease. The effects of exercise on sleep following caffeine intake generally improve sleep that is, stage 2, 3 and SWS increase and sleep onset latency decrease. A condition effect for sleep indicated sleep improvement after exercise Therefore The data supported a restorative theory of slow-wave sleep and suggest that acute exercise may be useful in promoting sleep and reducing sleep disturbance elevated by a high dose of caffeine.
XML Application for Bioinformatics
Journal of Life Science, volume 12, issue 4, 2002, Pages 383~391
DOI : 10.5352/JLS.2002.12.4.383
The difficulties in dealing with the Bioinformatics data come more from its idiosyncrasies than from its quantity Currently researchers need to an easy method for data exchange, manage, update. In order to integrate and manage all kinds of biological data, it is reasonable to adopt XML as standard tool since XML is independent of operating system, programming language and hardware platform. Although XML in Bioinformatics has been used widely as a standard notation abroad, however it is the beginning step in the domestic research. This article reviews a basic concept of XML and how to apply XML modeling in Bioinformatics. In addition we present XML applications for genomic sequences, structures and genetic network modeling.
Isolation of a Lignolytic Bacterium for Degradation and Utilization of Lignocellulose
Journal of Life Science, volume 12, issue 4, 2002, Pages 392~398
DOI : 10.5352/JLS.2002.12.4.392
38 strains were isolated in order to utilize lignin degrading ability from soil and compost. A organism having high lignin degrading ability of the isolated strains determined morphologcal and biochemical characteristics. Enrichment technique yielded a lignin degrading bacterium characterized as Pseudomonas sp. LC-2. This strain was able to degrade lignin which are the true representatives of native lignin and transform lignin to a lot of aromatic compounds as HPLC analysis of culture. By polyacrylamide gel analysis, it was determined that peroxidase consisted of three enzymes, with only one, the lignin peroxidase having high activity.
Analysis of Chloroplast Mutants of Arabidopsis Induced by Diepoxybutane
Journal of Life Science, volume 12, issue 4, 2002, Pages 399~406
DOI : 10.5352/JLS.2002.12.4.399
The Arabidopsis mutants involved in chloroplast development were induced by seed treatment of diepoxybutane which was rarely known mutagenic compound in plant mutagenesis. Three kinds of mutants designated as iml, gev, and yev were represented by the characteristics of variegated leaves, green vein with yellow leaves, and yellow green vein with green leaves respectively. We investigated the ultrastructure of chloroplast in mutated regions using transmission electron microscopy. The ultrastructure of chroloplast in wildtype showed regularly stacked grana thylakoid and stroma thylakoid while iml, gev and yev mutants displayed different shapes of grana stacking and stroma stacking of chloroplasts. Genetic analysis of three chloroplast mutants exhibit that divergent traits were ruled by a single recessive nuclear gene.
Survival of the early lavae of the Freshwater Crab, Eriocheir japonicus (De Haan) fed on different diets in the Laboratory
Journal of Life Science, volume 12, issue 4, 2002, Pages 407~415
DOI : 10.5352/JLS.2002.12.4.407
The survival of the early larvae of the freshwater crab, E. japonicus, fed on different diets at 22土1
1% were studied in the laboratory. The larvae in the control (no feed) lived for 4 days (1~2 zoeal stages), and in the experimental container of non-living foods (egg Powder, soy bean Powder, Pellet Powder, millet Powder, and mussel meat), they were lived for 7~9 days (2~3 zoeal stages), When the larvae fed on phytoplankton (Chlorella ellipsoid., Skeletonema costatum. and Chaetoceros gracilis), they lived for 10, 18, and 19 days(3~5 zoeal stages), and fed on zooplankton (Artemianauplii and rotifer), they were reached to the juvenile stage in 24 and 25 days, respectivily. When the larvae fed on a mixed diets (see table 1). it was more effective than a single food diet, and the most effective diets included Chaetoceros gracilis, Artemia nauplii and rotifera, in which the larvae reached the juvenile stage in 22 days, and the survival was 73%.
Biocontrol of Vegetables Damping-off by Bacillis ehimensis YJ-37
Journal of Life Science, volume 12, issue 4, 2002, Pages 416~422
DOI : 10.5352/JLS.2002.12.4.416
Bacillus ehimensis YJ-37 was observed as a potential biological agent to control the occurrence of diseases and plant growth.promoting rhizobacteria (PGPR). Population density of B. ehimensis YJ-37 were higher 1.2~2 times in main roots and lateral roots than from nonrhizosphere soil and persisted around 10
g root on the watermelon and radish root system upto 30 days after growing in pot condition. As a PGPR, B. ehimensis YJ-37 enhanced plant growth of watermelon and radish by soil treatment. The leaf area, hypocotyl length, root length and dry weight of radish were about 85, 33, 23 and 89% more than that of untreated plant, respectively. In case of watermelon were about 63, 27, 25 and 69% more than that of untreated plant, respectively. Biocontrol of damping-off in watermelon and radish caused by Rhizoctonia solani AG-4 and Pythium ultimum were carried out in pots using 3. ehimensis YJ-37. The results showed that might contribute to it`s suppression of damping-off disease in field plants.
Enantioselective Resolution for the Preparation of Chiral para-Nitrostyrene Oxide by Microbial Epoxide Hydrolase in an Organic Solvent
Journal of Life Science, volume 12, issue 4, 2002, Pages 423~426
DOI : 10.5352/JLS.2002.12.4.423
Enantioselective resolution of racemic para-nitrostyrene oxide was investigated using epoxide hydrolase activity of Aspergillus niger LK for the production of optically pure (S)-para-nitrostyrene oxide. To overcome the poor solubility of the substrate, enantioselective hydrolysis in an organic solvent was attempted under optimized reaction conditions including reaction temperature and water content. (S)-para-Nitrostyrene oxide with high optical purity (> 99% ee) was obtained at 37% yield using fungal epoxide hydrolase-catalyzed enantioselective resolution.
Sequence Analysis of Nuclear 18s rDNA from Porphyra dentata (Rhodophyta) in Korea
Long-Guo Jin ;
Journal of Life Science, volume 12, issue 4, 2002, Pages 427~432
DOI : 10.5352/JLS.2002.12.4.427
Nuclear 18S ribosomal RNA gene (18S rDNA or SSU rDNA) from the Porphyra dentata tissue was amplified and sequenced. Complete 18S rDNA has an 1822 bp exon and a 512 bp intron. The G+C contents of exon and intron were 49% and 55%, respectively. The exon sequence showed 97.1% homology to the GenBank accession number AB013183 of the Japanese P. dentata. The intron region that is inserted in upstream between 568 and 569 showed 52.1% homology to the AB013183.
An Analysis of Consumer`s Taste on Environment-Friendly Agricultural Products in Korea
Journal of Life Science, volume 12, issue 4, 2002, Pages 433~441
DOI : 10.5352/JLS.2002.12.4.433
There has been an increasing public concern about environmental safety and human health of field crops while customer consumes more and more field crops, to which overdose and residual chemicals were applied. As a solution for the problems, construction of sustainable agroecosystem is spreading out to pursuit the economic value of agricultural management as well as to meet environment concern. Public has extended their understanding on the preservation of environment and safeness of agricultural products, and governmental policy toward sustainable agriculture drives rapid increase of the production of sustainable agricultural products. Under this circumstances, it is time to encourage more consumption and to activate market system for the sustainable agricultural products. This study was initiated to diagnose the problems and future direction of domestic sustainable agriculture by analyzing the overall opinions of consumer on the sustainable agriculture. The results are as follows: The pursuit of low input management is a prerequisite to creat high valued agricultural products, and serious consideration should be taken to produce clean crops using natural products. Consummers are willing to pay 10∼50% more prices if the products get certified by official eco-label programs. It is believed that practice of sustainable agriculture using biopesticide and natural pesticide would accelerate the rapid extension of this pro-environmental agricultural management. To activate production and consumption of sustainable agricultural products correct informations on safety should be addressed to customer, and confidence has to be brought about from customer. This could be done by obtaining various and efficient distribution route, product competency for quality, upgraded sales strategy, maximum utilization of certificate system, and practice of recall system, so on.
Bromate Formation by Ozonation Process and It′s Effect on Renal Toxicity in rat
Journal of Life Science, volume 12, issue 4, 2002, Pages 442~451
DOI : 10.5352/JLS.2002.12.4.442
In oder to investigate the effects of pH and temperature on the formation of bromate ion, which is ozonation by-products of bromine containing natural water. At the same intial pH condition, the increase of pH shown similar trends even if the reaction variables such as temperature and reaction time of ozonation were changed. As pH and temperature were increasing, the bromate concentration was increased but bromine components (HOBr/OBr-) were decreased with increasing pH from 3 to 10. Lipid peroxide content in the kidney was increased by bromate which was ingestion with 0.4g/L for 24 weeks in drinking water. Renal cytosolic enzyme system (XO, AO) of bromate group were significantly increased in comparison with those of normal group. But microsomal enzyme system were not affected. BUN level and urinary
-glutamyltransferase activity were significantly increased in comparison with those of the normal. But, urinary lactate dehydrogenase activity was not affected. Renal glutathione content of rat was significantly decreased in comparison with those of normal rat given bromate. Renal glutathione S-transferase and
-glutamylcysteine synthetase activities were significantly decreased in bromate-treated group, but change in renal glutathione reductase activity was not significantly different from any other experimental group.
Effects of brefeldin A on spontaneous and delayed apoptosis of human neutrophils
Journal of Life Science, volume 12, issue 4, 2002, Pages 452~459
DOI : 10.5352/JLS.2002.12.4.452
Neutrophil apoptosis is a constitutive process that can be enhanced or delayed by various stimuli. In this study, effect of brefeldin A (BFA), which affects biological process of secretion, on constitutive and delayed apoptosis of neutrophils was investigated. Neutrophil apoptosis was determined after culturing for 20 hr in vitro by morphological changes, annexin V staining and DNA electrophoresis. BFA increased the constitutive apoptotic rate of neutrophils in dose-dependent manner. The delay of apoptosis induced by granulocyte macrophage-colony stimulating factor and lipopolysaccharide was also blocked by 10
M of BFA. However, this effect of BFA was less marked when neutrophils were treated with dexamethasone, interleukin-8, or dibutyryl-cAMP. Moreover, the delay of neutrophil apoptosis induced by rottlerin, a specific inhibitor of protein kinase C-
was significantly abrogated by BFA. Although BFA-induced apoptosis was not blocked by the caspase-3 inhibitor, zDEVD-fmk, expression levels of myeloid cell leukemia-1 (Mcl-1) were down-regulated by BFA. These results suggest that derangement of vesicular protein transport may be involved in the apoptosis of neutrophils, and that the action of BFA on apoptosis is dependent on changes in the expression of Mcl-1.
Production and characterization of anti-thyrog1obulin monoclonal antibodies
Journal of Life Science, volume 12, issue 4, 2002, Pages 460~463
DOI : 10.5352/JLS.2002.12.4.460
Twelve clones of monoclonal antibodies (mAbs) against thyroglobulin were produced and characterized. Among them, three mAbs (TN-1, TN-2 and TN-3) showed high binding affinity to thyroglobulin. from ELISA inhibition assay, TN-2 showed considerable reactivity with soluble thyroglobulin. TN-2 also reacted with phosphatidylserine which has a negative charge in aqueous condition. These results suggest that TN-2 has characteristics of autoantibody concerned with thyroiditis.
Effect of Substituted Residue 139 and 258 on Structural Changes of Mutant Tryptophan Synthase Pro
Lee, Joo-Youn ; Jeong, Jae-Kap ; Shin, Hae-Ja ; Lim, Woon-Ki ;
Journal of Life Science, volume 12, issue 4, 2002, Pages 464~468
DOI : 10.5352/JLS.2002.12.4.464
Enzymatic activities and fluorescence spectroscopic properties of the double mutant proteins P96L/F139W, P96L/F258W and a triple mutant protein P96L/F139W/F258W of tryptophan synthase
subunit from Escherichia coli was examined to study tertiary and local structure changes around the tryptophan residues. The enzymatic activities of P96l./F139W and P96L/F258W were similar, but P96L/F139W/F258W had lower activity, as compared to wild type. The fluorescence intensities of double mutant, P96L/F139W and P96L/F258W, were decreased but that of a triple mutant, P96L/F139W/F258W, was increased when compared to wild type. The sum of the maximum fluorescence intensity (fluorescence intensity at the λ
) for the double mutant proteins was not equal to the intensity seen in the triple mutant protein. The enzymatic activity and fluorescence data indicate that the replacement of Pro
longrightarrowLeu might affect on the stability of helix 8 and the loop located between strand 4 and helix4. The result suggests that the tertiary structure of triple mutant (P96L/F139W/F258W), being different from wild type, might have more compact residual structure at the vicinity of 139 and 258.8.
Up-regulation of Bax is associated with DNA topoisomerase I inhibitor β-lapachone-induced apoptosis in human prostate carcinoma cells
Journal of Life Science, volume 12, issue 4, 2002, Pages 469~476
DOI : 10.5352/JLS.2002.12.4.469
The DNA topoismerase I inhibitor
-lapachone, the product of a tree from South America, is known to exhibit various biological properties, however the mechanisms of which are poorly understood. In the present report, we investigated the effects of
-lapachone on the growth of human prostate carcinoma DU-145 cells. Upon treatment with
-lapachone, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and DNA fragmentation. Flow cytometry analysis confirmed that
-lapachone increased populations of apoptotic-sub Gl phase. In addition, proteolytic cleavages of poly (ADP-ribose) polymerase (PARP) and
-catenin protein were observed after treatment of
-lapachone. These apoptotic effects of
-lapachone in DU-145 cells were associated with marked induction of Bax protein, however the levels of Bcl-2 expression were decreased in a dose-dependent manner.
Expression of Biologically Active Insect-Derived Antibacterial Peptide, Defensin, in Yeast
Journal of Life Science, volume 12, issue 4, 2002, Pages 477~482
DOI : 10.5352/JLS.2002.12.4.477
As a biological model system for the production of an active antibacterial peptide, we have attempted the expression and secretion of insect defensin in Saccharomyces cerevisiae. Nucleotide sequences encoding mature defensin composed of 40 amino acids were fused in frame with promoter and signal sequence of Saccharomyces diastaticus glucoamylase, and mating factor
1] prosequence. The host strain, S. cerevisiae 2805 was transformed with the resulting plasmid, pSMFll The secretion of functional defensin was confirmed by growth inhibition zone assay using Micrococcus luteus as a test organism. Insect defensin was secreted to the culture supernatant in biologically active form by glucoamylase signal sequence and mating factor
1 prosequence. Most of antibacterial activity was detected in the culture supernatant. Defensin was also active against Staphylococcus aureus and Listeria monocytogenes.
Purification and Characterization of an Insect Antibacterial Peptide, Defensin, Expressed in Saccharomyces cerevisiae
Journal of Life Science, volume 12, issue 4, 2002, Pages 483~489
DOI : 10.5352/JLS.2002.12.4.483
We investigated the biochemical properties of insect defensin expressed and secreted from Saccharomyces corevisiae. The defensin showed extremely high resistance to boiling for up to 30 min and to pH values tested from 2.0 to 12.0. The treatment of defensin with various proteases abolished antibacterial activity. However, amylases, cellulase, lipase and catalase had no effect on the activity. The defensin was purified to homogeneity through ammonium sulfate concentration of culture supernatant, SP-Sepharose column chromatography and RP-HPLC. Tricin-SDS-PAGE analysis revealed that the molecular weight of the defensin was about 4.0 kDa. The antibacterial activity of the purified defensin was verified by renaturation of stained gel and gel pouring assay using Micrococcus luteus as a test organism.
Purification and characterization of biochemical properties of hemolysin from Vibrio fluvialis
Journal of Life Science, volume 12, issue 4, 2002, Pages 490~495
DOI : 10.5352/JLS.2002.12.4.490
Hemolysin (VFH) of V. fluvialis, which is a pathogenic bacteria, causing watery diarrhea with vomiting, abdominal croup, was purified. V. fluvialis was cultivated in BHI medium and the culture supematant was precipitated by ammonium sulfate. The protein was purified by chromatographies on columns of DEAE-cellulose and Mono-Q. Molecular weight of the purified VFH was estimated as 79kDa by SDS-PAGE. The optimal temperature for a maximum hemolytic activity was at around 35
and the activity was decreased at 4
Cytotoxicity of VFH was also investigated using RTG-2 cell line. LDH assay study showed that 50
/m1 of VFH release 80% of total cellular LDH (lactate dehydrogenase) from RTG-2 cell and microscopic observation also showed the morphological change of cell.
Development of a Recombinant Strain of Pichia pastoris with Antibacterial Activity
Journal of Life Science, volume 12, issue 4, 2002, Pages 496~503
DOI : 10.5352/JLS.2002.12.4.496
To develop a yeast strain of Pichia pastoris producing an antibacterial peptide, we have attempted the expression and secretion of an insect defensin. The nucleotide sequences corresponding to mature defensin were chemically synthesized by 6 oligomers, assembled in vitro and the synthesized gene was identified by nucleotide sequencing. The prepro sequence of yeast mating factor
1 and the defensin gene were recombined into a Pichia expression vector, pPIC9K. The resulting plasmid, pPIDE, was transformed into P. pastoris GSl15 and transformants selected on histidine-deficient minimal plates were tested for antibacterial activity against Micrococcus luteus. Four strains with different antibacterial activity were selected for further analysis. Southern hybridization and RT-PCR verified the defensin gene was maintained and transcribed in a host. Four strains were cultivated in YPD broth for 96 hours to compare cell growth and antibacterial activity, They showed no difference in cell growth, however, each strain showed different antibacterial activity pattern with culture time. The maximal activity was about 550 AU/