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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 13, Issue 6 - Dec 2003
Volume 13, Issue 5 - Oct 2003
Volume 13, Issue 4 - Aug 2003
Volume 13, Issue 3 - Jun 2003
Volume 13, Issue 2 - Apr 2003
Volume 13, Issue 1 - Feb 2003
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Enhanced Sensitivity and Long-Term G
/M Arrest in Adriamycin-treated DNA-PK-null Cells are Unrelated to DNA Repair Defects
;;;Dang Van Cuong;;;
Journal of Life Science, volume 13, issue 3, 2003, Pages 241~247
DOI : 10.5352/JLS.2003.13.3.241
While the DNA-protein kinase (DNA-PK) complex, comprised of DNA-PKcs and Ku80, is primary involved in the repair of DNA double-strand breaks, it is also believed to participate in additional cellular processes. Here, treatment of embryo fibroblasts (MEFs) derived from either wild-type (Wt) or DNA-PKcs-null (DNA-
) mice with various stress inducing agents revealed that adriamycin was markedly more cytotoxic for
and led to their long-term accumulation in the
/M phase. This differential response was not due to differences in DNA repair, since adrimycin-triggered DNA damage was repaired with comparable efficiency in both Wt and
, but was associated with differences in the expression of important cell cycle regulatory genes. Our results support the notion that Ku80-mediated cytoprotection and
/M-progression are not only dependent on the cell`s DNA repair but also may reflect Ku80`s influence on additional cellular processes such as gene expression.
Dimerization of Thyroglobulin in the Endoplasmic Reticulum
Journal of Life Science, volume 13, issue 3, 2003, Pages 248~251
DOI : 10.5352/JLS.2003.13.3.248
The kinetics of dimerization of a newly synthesized thyroglobulin (Tg), the precursor protein in the manufacture of thyroid hormone, was investigated in the endoplasmic reticulum of thyrocytes FRTL-5 cell line. The folded monomeric Tg was first detectable in a conformationally unstable form, from the examination of lysates of pulse labeled cultured thyrocytes by denaturing and nondenaturing gel electrophoresis by 15 min after biosynthesis. The first dimeric Tg was formed by 30 min after; the monomer declined and the dimer progressively increased, and 40 min after remarkable dimeric Tg form was found. Finally, dimerization was complete at 60 min after.
Analysis of deleterious genes in natural and experimental populations of Drosophila melanogaster
Journal of Life Science, volume 13, issue 3, 2003, Pages 252~257
DOI : 10.5352/JLS.2003.13.3.252
The genetic variabilities of second chromosomes of Drosophila melanogaster concealed in Busan natural and experimental populations have been analyzed by the Cy//Pm method. The experimental population was composed of D. melanogaster which had the lethal-free second chromosome, collected Sasang natural population in 1982. The frequencies of deleterious genes were estimated to be 14.3∼25.4% in Busan natural population and 26.5∼27.2% in experimental population. The allelism rates in lethal genes isolated from the natural and experimental populations were calculated to be about 0.76% and 9.76∼14.17%, respectively. The value of elimination by the frequencies of deleterious genes and allelism rate was 0.0106and the effective population size estimated to be about 430 flies at the 6570 days population.
Development of an Agar-gel Added Soy Beverage
Journal of Life Science, volume 13, issue 3, 2003, Pages 258~264
DOI : 10.5352/JLS.2003.13.3.258
An agar-gel added soy beverage was developed. Optimum processing conditions for preparation of agar-gel were 1.5% agar with 0.1-0.2% sodium chloride and 0.1% glucomannan. And optimum manufacture conditions for soy juice were 10% solid seasoned with 3% sugar and 0.1-0.2% salt. The result of sensory evaluation revealed that overall acceptability of soy beverage with 10% solid seasoned with 3% sugar and 0.1-0.2% salt and agar-gel made with 1.5% agar, 0.2% sodium chloride and 0.1% glucomannan exceeded other groups of samples.
Effect of Liquid Cultures of Cordyceps militaris on Lipid Metabolism and Enzyme activities in hyperlipidemic Female Rats
Journal of Life Science, volume 13, issue 3, 2003, Pages 265~272
DOI : 10.5352/JLS.2003.13.3.265
The effect of liquid cultures of Cordyceps militaris (LCM) on weight gain, food intakes, food efficiency ratios, serum and hepatic lipids, fecal lipids excretion, serum protein and enzyme activities, were investigated in adult female rats (30 weeks old). Sprague-Dawley rats were assigned to one normal and four hyperlipidemic diet groups, Hyperlipidemic diet groups (20% fat, 1% cholesterol) were divided into high fat diet (LCM free water), 10%, 20% or 30% LCM diet groups (10%, 20% or 30% LCM in water) according to the levels of LCM supplementation. After 35 days of experimental diet consumption, the body weight gains, hepatic weights, and food efficiency ratios of the rats fed hyperlipidemic diets were significantly increased compared with those of the rats fed normal diet. The concentrations of serum and hepatic triglycerides, hepatic total lipid, and atherogenic index of the rats fed 20% or 30% LCM diets were significantly lower than those of the rats fed the high fat diet. The concentration of serum HDL-cholesterol of the rats fed all LCM diets was significantly higher than those of the rats fed the high fat diet. The fecal excretion of triglyceride in the rats fed 20% or 30% LCM diets was significantly higher than those of the rats fed high fat diet. The concentrations of serum and hepatic total cholesterol, serum LDL-cholesterol, and HDL-cholesterol/total cholesterol ratio, fecal excretion of cholesterol, and the activities of serum glutamic pyruvic transaminase and alkaline phosphatase of the rats fed all LCM diets were similar to those of the rats fed high fat diet. No differences were noted in the weights of kidney and femur, the serum concentration of glucose, total protein and albumin, and the activities of glutamic oxaloacetic transaminase and
-glutamyltranspeptidase, among the rats on all the experimental diets. These results showed that the 20% or 30% LCM diets feeding decreased the serum and hepatic triglycerides, and the atherogenic index, and increased the serum HDL-cholesterol of the adult female rats.
Changes in Major Taste Components of Soybean Sprout Germinated with Extract of Korean Panax ginseng
Journal of Life Science, volume 13, issue 3, 2003, Pages 273~279
DOI : 10.5352/JLS.2003.13.3.273
Changes in weight, length, amino acids, organic acids and free sugars of soybean sprouts germinated with extract of Panax ginseng(PGE, 100∼400 ppm) were investigated. PGE increased the weight and length of soybean sprouts. Content of total amino acid in soybean sprout germinated at 100 ppm of PGE after cultivation for 3 days was the most abundant and then decreased by increasing the concentration of PGE. Content of aspartic acid was increased with culture time, but that of glutamic acid was shown to be an opposite trend. Content of total free sugar was increased by increasing culture time and not affected by concentration of PGE. Content of sucrose in control group during growth of soybean sprout was decreased, but sucrose contents in PGE groups were increased to 3 days and decreased thereafter. However, the other sugars were continuously increased for 4 days. Content of total organic acids was the most abundant in soybean sprouts germinated with 200 ppm of PGE and cultured for 3 days. Phytic acid was a major organic acid, showing the range of 45 to 60% for total organic acids. In conclusion, PGE as sprouting water of soybean was effective to increase of growth, contents of amino acids and organic acids in soybean sprouts, indicating that PGE accelerated the quality of soybean sprouts.
Incorporation of RAPD linkage Map Into RFLP Map in Glycine max (L, ) Merr
Choi, In-Soo ; Kim, Yong-Chul ;
Journal of Life Science, volume 13, issue 3, 2003, Pages 280~290
DOI : 10.5352/JLS.2003.13.3.280
The incorporation of RAPD markers into the previous classical and RFLP genetic linkage maps will facilitate the generation of a detailed genetic map by compensating for the lack of one type of marker in the region of interest. The objective of this paper was to present features we observed when we associated RAPD map from an intraspecific cross of a Glycine max
G. max, `Essex`
PI 437654 with the public RFLP map developed from an interspecific cross of G. max
G. soja. Among 27 linkage groups of RAPD map, eight linkage groups contained probe/enzyme combination RFLP markers, which allowed us the incorporation of RAPD markers into the public RFLP map. Map position rearrangement was observed. In incorporating L.G.C-3 into the public RFLP linkage group a1 and a2, both pSAC3 and pA136 region, and pA170/EcoRV and pB170/HindIII region were in opposite order, respectively. And, pk400 was localized 1.8 cM from pA96-1 and 8.4 cM from pB172 in the public RFLP map, but was localized 9.9 cM from i locus and 18.9 cM from pA85 in our study. A noticeable expansion of the map distances in the intraspecific cross of Essex and PI 437654 was also observed. Map distance between probes pA890 and pK493 in L.G.C-1 was 48.6 cM, but it was only 13.3 cM in the public RFLP map. The distances from the probe pB32-2 to pA670 and from pA670 to pA668 in L.G. C-2 were 50.9 cM and 31.7 cM, but they were 35.9 cM and 13.5 cM in the public RFLP map. The detection of duplicate loci from the same probe that were mapped on the same or/and different linkage group was another feature we observed.
Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain
Kim, Heui-Soo ; Jeon, Seung-Heui ; Yi, Joo-Mi ; Kim, Tae-Hyung ; Lee, Won-Ho ;
Journal of Life Science, volume 13, issue 3, 2003, Pages 291~297
DOI : 10.5352/JLS.2003.13.3.291
A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FBl2) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.
Genetic Composition Analysis of Marine-Origin Euryarchaeota by using a COG Algorithm
Journal of Life Science, volume 13, issue 3, 2003, Pages 298~307
DOI : 10.5352/JLS.2003.13.3.298
To figure out the conserved genes and newly added genes at each phylogenetic level of Archaea, COG (clusters of orthologous groups of proteins) algorithm was applied. The number of conserved genes within 9 species of Archaea was 340 and that of 8 species of Euryarchaeota was 388. Many of conserved 265 COGs, which are specific to Archaea and absent in Bacteria and S. cerevisiae, were concerned with `information storage and processing` (94 COG, 35.5%) and `metabolism` (82 COG, 30.9%). COGs related to these functions were assumed as highly conserved and permit peculiar life form to Archaea. It seemed that there was some difference in `nucleotide transport and metabolism` and there was little difference in `information storage and processing` between Euryarchaeota and Crenarchaeota. Marine-origin Euryarchaeota showed different conserved COGs with terrestrial Euryarchaeota. Conserved COGs, related to carbohydrate transport and metabolism and others, were different between marine- and terrestrial-origin Euryarchaeota. Hence it was assumed that their physiology might be different. This study may help to understand the origin and conserved genes at each phylogenetic level of marine-origin Euryarchaeota and may help in the mining of useful genes in marine Archaea as Manco et al. (Arch. Biochem. Biophy. 373, 182 (2000)).
Isolation and Characterization of Recombinant Vibrio parahaemolyticus Collagenase
Journal of Life Science, volume 13, issue 3, 2003, Pages 308~313
DOI : 10.5352/JLS.2003.13.3.308
The collagenase gene from Vibrio parahaemolyticus 04 was subcloned into an expression vector pET-29b. The recombinant collagenase was expressed in Escherichia coli BL2l(DE3) and partially purified by Hi-Trap affinity and Sephacryl S-100 size exclusion chromatographies. The recombinant enzyme was purified by 43.7-fold and the yield was 73%. SDS-PAGE revealed that the molecular weight of the enzyme was approximately 35 kDa. Substrate specificity study of the enzyme displayed that the enzyme showed the highest activity with the type I collagen and the synthetic peptide, Z-GPGGPA, indicating that the enzyme was indeed a collagenase. The enzyme showed broad pH optimum around pH 6-12 and was stable between pH 5.5 and 11.5. The optimum temperature for the type I collagen degradation was
. The thermostability measurement of the enzyme indicated that the enzyme was stable up to
, but the activity was diminished quickly above
Comparison and Analysis between Human Breast Cancer Cells and Hepatoma Cells for the Effects of Xenobiotic Nuclear Receptors (Constitutive Androstane Receptor, Steroid and Xenobiotic Receptor, and Peroxisome-Proliferator-Activated Receptor γ ) on the Transcriptional Activity of Estrogen Receptor
Journal of Life Science, volume 13, issue 3, 2003, Pages 314~323
DOI : 10.5352/JLS.2003.13.3.314
The purpose of this study was to examine the effects of xenobiotic nuclear receptors, CAR, SXR, and PPAR
on the transcriptional activity of estrogen receptor in human breast cancer cell lines and compare with those in human hepatoma cell line. Two different breast cancer cell lines, MCF-7 and MDA-MB-231 were cultured and effects of CAR, SXR, and PPAR
on the ER-mediated transcriptional activation of synthetic (4ERE)-tk-luciferase reporter gene were analyzed. Consistent with the previous report, CAR significantly inhibited ER-mediated transactivation and SXR repressed modestly whereas the PPAR
did not repress the ER-mediated transactivation. However, in breast cancer cells neither of the xenobiotic receptors repressed the ER-mediated transactivation. Instead, they tend to increase the transactivation depending on the cell type and xenobiotic nuclear receptors. In MCF-7, SXR but neither CAR nor PPAR
slightly increased ER-mediated transactivation whereas in MDA-MB-231, CAR and PPAR
but not SXR tend to increase the transactivation of the reporter gene. These results indicate that the effects of ER cross-talk by the CAR, SXR, and PPAR
, are different in breast cancer cells from hepatoma cells. In conclusion, the transcriptional regulation by estrogen can involve different cross-talk interaction between estrogen receptor and xenobiotic nuclear receptors depending on the estrogen target cells.
Effects of Constitutive Androstane Receptor (CAR) on PBRU Transactivation of CYP2B Gene in Different Culture Cell Types: Comparison Between Hep G2 and COS-cells
Journal of Life Science, volume 13, issue 3, 2003, Pages 324~332
DOI : 10.5352/JLS.2003.13.3.324
The objective of this study was to examine if transient transfection of CAR can transactivate CYP2B1 PBRU reporter gene in COS cells in which the endogenous CYP2B1 gene is not induced by PB. In non-transfeced cells of both Hep G2 and COS, the endogeneous expression of CAR was not detected by antibody against CAR. When cultured cells were transfected with CAR expression plasmid, mCAR1-GFP, both cell types expressed high levels of CAR protein and could allow to examine the effect of CAR in PBRU transactivation. Both cell types expressed endogenous RXR and transfection of RXR expression plasmid dramatically increased its protein expression. Whereas CAR transactivated PBRU2C1Luciferase about 12 fold as compared to 2C1Luciferase in Hep G2 cells, it did not stimulate the luciferase activity of the PBRU reporter gene in COS cells. These results indicate that Hep G2 cells can respond to CAR differently from COS cells, and suggest that factors other than CAR and RXR may be required in inducing PBRU activation and the expression of these factors may be different between liver and kidney.
Effect of buchu (Allium tuberosum) on lipid peroxidation and antioxidative defense system in streptozotocin-induced diabetic rats
Journal of Life Science, volume 13, issue 3, 2003, Pages 333~342
DOI : 10.5352/JLS.2003.13.3.333
The pathogenic effort of high glucose, possibly in concert with fatty acids, is mediated to vascular complications of diabetes via increased production of reactive oxygen species(ROS), reactive nitrogen species(RNS), and subsequent oxidative stress. This study was carried out to investigate the suppressive effect of buchu(Allium tuberosum) on oxidative stress in streptozotocin(STZ)-induced diabetes in Sprague Dawley male rats. The effect of buchu supplementation (10%) on lipid peroxidation, and antioxidative defense system in blood and liver was compared among normal rats fed basal diet(normal) and diabetic rats fed basal diet(DM-control) or 10% buchu-supplemented diet(DM-buchu). Diabetes was experimentally induced by the femoral muscle injection of 50 mg STZ per kg of body weight. Animals were sacrificed after 4 wks of experimental diets feeding. The induction of diabetes by STZ elevated the level of lipid peroxidation represented by thiobarbituric acid-reactive substances(TBARS) and conjugated dienes in plasma, LDL, liver, and erythrocytes. 10% buchu-supplemented diet significantly reduced the levels of conjugated dienes in erythrocytes(p<0.05) and lowered TBARS in liver and LDL to the levels of control. Induction of diabetes by STZ elevated Mn-superoxide dismutase(Mn-SOD) activity and lowered activities of glutathionine reductase(GSH-red) and glutathionine peroxidase(GSH-px). Catalase activity was not affected by the induction of diabetes by STZ. However, buchu supplementation to diabetic rats significantly elevated catalase activity(p<0.05) and slightly elevated GSH-px and GSH-red activities in liver. GSH levels of blood and liver were lowered or not changed by induction of diabetes by STZ, respectively, while buchu supplementation to diabetic rats significantly elevated hepatic GSH level (p<0.05). In conclusion, it can be concluded that buchu might be a food source to attenuate oxidative stress in diabetic patients by inhibiting lipid peroxidation, by increasing hepatic GSH level, and by inducing anti-oxidative enzyme systems.
Assessment and Classification of Korean Indigenous Corn Lines by Application of Principal Component Analysis
Journal of Life Science, volume 13, issue 3, 2003, Pages 343~348
DOI : 10.5352/JLS.2003.13.3.343
This study was conducted to get basic information on the Korean local corn line collected from Busan City and Kyungnam Province, a total of 49 lines were selected and assessed by the principal component analysis method. In the result of principal component analysis for 7 characteristics, 67.4% and 86.3% of total variation could be appreciated by the first two and first four principal components, respectively. Contribution of characteristics to principal component was high at upper principal components and low at lower principal components. Biological meaning of principal component and plant types corresponding to the each principal component were explained clearly by the correlation coefficient between principal component and characteristics. The first principal component appeared to correspond to the size of plant and ear, and the duration of vegetative growing period. The second principal component appeared to correspond to the number of ear and tiller. But the meaning of the third and fourth principal components were not clear.
Processing Conditions of Functional Anchovy Snack
Journal of Life Science, volume 13, issue 3, 2003, Pages 349~354
DOI : 10.5352/JLS.2003.13.3.349
In order to manufacture the high-quality products as well as to promote their consumption, processing conditions for anchovy snacks were investigated. Seasoning juice-1 were made for 7% sugar, 2% salt, 5% soy sauce, 2% ginger, 1% pepper, 5% garlic, 5% onion, 2% glasswort extract and 0.5% taurine with water. Seasoning juice-2 were made for 15% frying powder, 15-20% starch syrup, 10% soybean powder and 2% sesame powder with water. Sensory evaluation revealed that overall acceptability of anchovy snacks with 30% moisture before popping, 15% frying powder and 15-20% starch syrup in seasoning juice-2 with 20 seconds popping time exceeded other groups of samples.
Screening of Potent Biofungicide for the Growth Inhibition of Soilborne Pathogenic Fungi, Rhizoctonia solani
Journal of Life Science, volume 13, issue 3, 2003, Pages 355~358
DOI : 10.5352/JLS.2003.13.3.355
Various Trichoderma spp. were evaluated for the development of biofungicides to control soilborne pathogen, Rhiztonia solani, Various Trichoderma spp. were initially tested for their ability to inhibit growth of R. solani by inhibition zone test. Inhibition zones of 3∼5 mm toward R. solani were detected on PDA agar plates. The parasitic activity of strains, the activities of cell-wall-degrading enzymes such as glucanases and chitinases, were also evaluated. Highest activities of glucanase and chitinase were 3.5 U/ml and 0.9 U/ml, respectively, Isolated Trichoderma spp. also exhibited good growth with currently used agrochemicals, which represents that the isolated biofungicides can be mutually used with agrochemicals.
Characteristics of Sexual Maturation in the Seaweed Porphyra pseudolinearis from East Sea, Korea
Journal of Life Science, volume 13, issue 3, 2003, Pages 359~364
DOI : 10.5352/JLS.2003.13.3.359
The seaweed Porphyra pseudolinearis as a dominant species grows at upper of the intertidal zone of the East Sea from October to March. Vegetative cells have not matured during October when observed with naked eye and microscope. In the middle of November, although it didn`t distingush between female and male gametophytes by eye, it showed antheridium and carpogonium when observed vertical section under microscope. From early December, It could be distinguished female and male gametophytes clearly. From Feburary it showed shorted length of thalli by release of spermatangia and carposporangia as maturation. At early March, the colar has been decaeded and side of thalli has been melted, completed of release of spermatia. At the end of December, the average length and width of female thalli were 149.9
5.6mm and 22.2
2.3mm, respectively. The length and width of male thalli were 149.9
9.4mm and 20.7
1.8mm. At the end of January, the average length and width of female thalli were 94.6
6.4mm and 29.1mm
5.1, respcetively. The length and width of male thalli were 107.8
7.3mm and 25.9
0.9mm. From this period lengths of female and male thalli have already been shortned by the release of spermatia and carpogonia.
Deodorization of Fish Oil Using Adsorption Method
Journal of Life Science, volume 13, issue 3, 2003, Pages 365~373
DOI : 10.5352/JLS.2003.13.3.365
Instead of deodorization appararus of fish oil, an adsorbent such as activated charcoal, activated alumina, silicagel, bamboo charcoal was packed in column alone or mixed with preparative ratio, and then test the effective deodorization with bleaching. In the progress of degumming, the effective method was 18 ml of 2.5% oxalic acid per 100m1 of crude large anchovy oil. The optical condition to deacidified was treating for 30 min at
with 2.5% sodium hydroride solution. The effective deodorization was added with 3% silicagel under the alone treating adsorbent, and mixed treating was 30% activated alumina and 10% silicagel but added to green tea powder was not effective. The major fatty acid of total lipid were 16:0, 20:5n-3, 18:1n-9, 16:1n-7 and 22:6n-3 after treatment of degumming, deacidfication and deodorizing in the large anchovy oil. The oxidative stability of refined anchovy oil added to
-tocopherol was validated 20 days under the control, and 30 days in the case of
-tocopherol. The 0.01%
-tocopherol was more effective than 0.02%