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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 13, Issue 6 - Dec 2003
Volume 13, Issue 5 - Oct 2003
Volume 13, Issue 4 - Aug 2003
Volume 13, Issue 3 - Jun 2003
Volume 13, Issue 2 - Apr 2003
Volume 13, Issue 1 - Feb 2003
Selecting the target year
Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 14
Choi, In-Soo ; Kim, Yong-Chul ;
Journal of Life Science, volume 13, issue 4, 2003, Pages 375~382
DOI : 10.5352/JLS.2003.13.4.375
A direct and precise explanation of soybean resistance to soybean cyst nematode will be possible only when the individual gene(s) involved in the resistance are tagged. This study was conducted, (1) to identify and localize quantitative trait loci for resistance to soybean cyst nematode race 14 on RAPD map, (2) to identify the magnitude and mode of inheritance for each quantitative trait loci, and (3) to identify the best combinations of quantitative trait loci for resistance to soybean cyst nematode race 14. Thirty markers (29 RAPD and 1 RFLP) showed significant association with resistance to soybean cyst nematode race 14. From MAPMAKER/QTL analysis, we identified two regions (linkage group C-7 and linkage group C-9) for resistance to soybean cyst nematode .ace 14. The first quantitative trait loci that was localized at 6.0 cM from
on linkage group C-7 showed a dominant inheritance mode. However, we can not exclude the possibility of additive inheritance mode. The second quantitative trait loci that was localized between
on linkage group C-9 also showed a dominant mode of inheritance. One pair of flanking markers (
) and B15
were used for multiple regression analysis. Marker combination that included 2 markers,
, explained the highest total variance (22.9%) for resistance to soybean cyst nematode race 14. Further localization of genes for resistance to soybean cyst nematode race 14 and examination of interaction between quantitative trait loci will accelerate the exploitation of resistance to soybean cyst nematode.
Characterization and functional analysis of a myo-inositol 1-phosphate synthase cDNA in sesame (Sesamum indicum L.)
Journal of Life Science, volume 13, issue 4, 2003, Pages 383~389
DOI : 10.5352/JLS.2003.13.4.383
A cDNA (SeMIPS) encoding myo-inositol 1-phosphate synthase has been isolated from developing sesame (Sesamum indicum L. cv. Dan-Baek) seeds and its structure and function analyzed. The SeMIPS protein was highly homologous with those from plant species (88-94%), while a much lower degree of sequence homology (60%) was found with that of human. The functional domains commonly found in MIPS protein were identified and their amino acid residues were compared with each other. Northern blot indicated that the expression of the SeMIPS gene might be organ-specifically regulated. A complementation assay based on a yeast mutant system confirmed that the SeMIPS gene encodes a myo-inositol 1-phosphate synthase (MIPS) of sesame by showing functional expression of the SeMIPS cDNA in the yeast mutants containing the disrupted INO1 gene.
Bacteriological Study of Listeria sp. Isolated from Seawater and Sea Food
Journal of Life Science, volume 13, issue 4, 2003, Pages 390~399
DOI : 10.5352/JLS.2003.13.4.390
Four species of the genus of Listeria were isolated from seawater and sea food in Kyungnam province, South Korea. These isolated strains were classified into Listeria sp. from different samples by appropriate cultivation conditions and biochemical tests including serological test. In a day enrichment cultivation, the following strains were found out of 100 samples: L. innocua (35%), L. ivanovii (4%), L. monocytogenes (4%), and L. welshimeri (1%). For seven days enrichment culture, L. innocua (38%), L. ivanoii (5%), L. monocytogenes (7%), and L. welshimeri (1%) were isolated. From these results, Listeria species were more efficiently isolated in seven day enrichment broth than in one day enrichment. However, these isolated Listeria species were less grown in the selective medium than in the enrichment medium. Isolation rates of Listeria species showed differency for each sample and Listeria species were more abundantly isolated in shrimps (80%) and crayfishes (80%) than little neck clams (50%), seawater (25%) and mussels (20%). From the results of serological classes for the seven L. monocytogenes, two strains were defined as type I and the other five strains as type IV.
Phylogenetic Analysis of the Entomopathogenic Fungal Species and Taxonomical Positions of Their Commercial Products
Journal of Life Science, volume 13, issue 4, 2003, Pages 400~411
DOI : 10.5352/JLS.2003.13.4.400
This study was carried out to identify the phylogenetic relationship and to know the distribution of the entomopathogenic fungi by comparing the DNA sequences of internal transcribed spacer regions (ITS1 and ITS2) and 5.8S ribosomal DNA (rDNA) repeat unit. The entomopathogenic fungi had their specific sequences in ITS1 and 2 regions depending on species. The comparison of the ITS sequences of standard strains indicated that the sequences ITS1 were more variable than those of ITS2. It seems that Paecilomyces tenuipes, Isaria japonicus and P. japonicus are the same species but called as different names because of very similar sequences, and unidentified Paecilomyces sp. KACC 40220 and KACC 40656 showed identical sequences to P. tenuipes. Thirty six strains of the commercial products of entomopathogenic fungi used in this study were divided into four groups by the phylogenetic analysis based on 5.85 rDNA and ITS regions. We found twenty-three strains were P. tenuipes / japonica, eleven strains were C. militaris, and other two strains were Beauveria bassiana and C. multiaxialis, respectively.
Detection of Silent Allele at Esterase(Es) Locus in Jeju Native Horse
Journal of Life Science, volume 13, issue 4, 2003, Pages 412~415
DOI : 10.5352/JLS.2003.13.4.412
The purpose of this present study was to investigate the polymorphism of esterase locus for individual identification and parentage verification in Jeju native horse (JNH). Seventy three random JNH samples were studied by polyacrylamide gel isoelectric focusing(IEF) at pH 3.5 ∼ 6.0. We detected international recognized alleles, F, G, H, I, M, and an silent allele
. Gene frequencies of allele I showed 0.479 the highest, while allele H and M(
) with relatively low frequencies were 0.027 and 0.014, respectively.
A Case of Parentage Testing in Dog by Microsatellite DNA Typing
Journal of Life Science, volume 13, issue 4, 2003, Pages 416~420
DOI : 10.5352/JLS.2003.13.4.416
We performed this study to determine the parentage verification of putative dogs. A total of 7 samples (4 Labrador Retriever dog and 3 Poongsan dog) were genotyped by using 12 international markers (PEZ1, PEZ5, PEZ8, PEZ10, PEZ11, PEZ12, PEZ17, PEZ20, PEZ21, FHC2010, FHC2054, FHC2079). This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Labrador Retriever Pup I and Pup II were included according to principles of Mendelian genetics in all loci, while Poongsan Pup III was excluded with markers PEZ1 (106/118), PEZ10 (276/300), and FHC2010 (228/232). These results suggest that the present DNA typing is so useful for parentage verification of these two breeds.
Characteristics of Heavy Metal Biosorption by Enterobacter intermedious KH410
Journal of Life Science, volume 13, issue 4, 2003, Pages 421~427
DOI : 10.5352/JLS.2003.13.4.421
A natural habit at bacterium, Enterobacter intermedious KH410 was isolated from freshwater plant root and identified. Adsorption of heavy metals such as lead, cadmium, and copper by this strain was examined. The minimal inhibitory concentrations(MIC) for each metal were 1.78 mM for lead, 0.17 mM for cadmium and 1.39 mM for lopper, respectively. Maximum production of dried cell was 2.56 g/
in LB medium containing 0.5% NaCl, 1% yeast extract and 1% of lactose. Optimal conditions for adsorption were 0.6 dry g-biomass, at pH 4.0 and the temperature of
. Adsorption equilibrium reached maximum after 30 min in 400 mg/
metal solution. The adsorption capacity (K) of copper was 1.5 times higher than that of cadmium and lead was 1.1 times higher than that of cadmium. from the results obtained in this study, Freundlich adsorption model was applicable for all metals. Adsorption strength (1/n) of heavy metal ions were in the order of cadmium>copper>lead. The adsorption of dried cell for lead, cadmium, and copper was 56.2, 58.0, 55.8 mg/g-biomass, respectively. Pretreatment to increase ion strength was the most effective with 0.1 M NaOH whereas slight difference was found both KOH and
upon same concentration. Effective desorption was induced by 0.1 M EDTA for lead and 0.1 M
for cadmium and copper.
Effect of Seed Pelleting on the Precision Planting and Seedling Emergence of Carrot Seeds
Journal of Life Science, volume 13, issue 4, 2003, Pages 428~432
DOI : 10.5352/JLS.2003.13.4.428
This study was carried out to develop and investigate integrated mechanization system for culture of carrot. Compared with the conventional hand planting, use of a mechanical planter for pelleted seeds resulted in a saving of planting time by 225 hours. An additional saving of 460 hours was obtained for thinning the seedlings. Besides seeds could be saved by 72%, planting and thinning be done only in 35 hours per ha field when the pelleted seeds were planted with a planter. Work hour reduced by adopting the mechanical planting of pelleted seeds sharply contrasted to that of 720 hours required for hand-planting the thinning hours, resulting in a reduction of labor by more than 95%. SMP followed pelleting tended not only to increase the percentage of seedling emergence in the field but to induce an a day earlier germination.
Effect of Osmotic Priming and Solid Matrix Priming to Improved Seed Vigor and Early Growth of Pepper and Tomato Seeds
Journal of Life Science, volume 13, issue 4, 2003, Pages 433~440
DOI : 10.5352/JLS.2003.13.4.433
Osmotic and solid matrix priming treatments enhanced germination performance. We compared osmotic with solid matrix priming to determine the more effective treatment for improving seed germination in pepper and tomato. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in the two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage (
) compared with untreated seeds, regardless of germination temperature.
value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and
, respectively. While, that in tomato seeds was reduced about 3.3, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and
, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the
was greater when the seeds were germinated at
than at temperature of higher than
. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference on the percentage germination between solid matrix and osmotic primed seeds in tomato. After priming, dried-bark seeds showed faster germination than surface-dried seeds in pepper. On the other hand, there was no significant difference in tomato. Emergence of pepper and tomato seeds was markedly enhanced by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of pepper and tomato seeds.
Inhibitory Effect of Phorbol 12-Myristate 13-Acetate on NO Production Induced by Interleukin-1 beta in Aortic Vascular Smooth Muscle Cells of Rats
Journal of Life Science, volume 13, issue 4, 2003, Pages 441~447
DOI : 10.5352/JLS.2003.13.4.441
To examine the role of protein kinase C (PKC) in regulation of interleukin-1 beta (IL-1
)-induced iNOS expression, IL-1
-induced nitrite production was observed in cultured vascular smooth muscle (VSM) cells pretreated with phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-butyrate (PDB) as PKC activator; 4
-phorbol-didecanoate (PDD) as PKC non-activator. Nitrite production induced by IL-1
was increased by the presence of increasing concentration of PMA ranging from 2 to 200 nM. However, in VSM cells pretreated with PMA and PDB, IL-1
production was decreased in proportion to the duration of pretreatment, and most significantly decreased in pretreatment time of 24 hours. Using RT-PCR method, the expression of iNOS mRNA induced by IL-1
was decreased in VSM cells pretreated with PMA 200 nM for 24 hours. These results suggest that decrease in IL-I
-induced nitrite production by the pretreatment of PMA result from inhibition of iNOS expression and the inhibition related to PMA-induced PKC down-regulation.
The Effect of Aerobic Exercise and CWT on Cardiorespiratory Function and Body Composition For Female Workers
Lee, Jae-Gue ;
Journal of Life Science, volume 13, issue 4, 2003, Pages 448~456
DOI : 10.5352/JLS.2003.13.4.448
The purpose of this study was to determine changes in their cardiorespiratory function and body composition in female workers after aerobic exercise and circuit weight training. The subjects of this study were 16 female workers in B city and was divided into exercise and control groups which were composed of 8 people respectively. The members of exercise group had aerobic exercise and circuit weight training an hour a day, 5 days per week, for 8 weeks while controled group did not. Both groups were pretested and posttested, and the results of this study on cardiorespriatory function and body composition are as follows. Exercise group showed significant decrease in heart rate at rest(p<.001). Exercise group showed significant increase in the oxygen uptake at rest, in the maximum oxygen uptake and in the vital capacity(p<.001). Exercise group showed significant decrease in the %fat and fat(p<.001). Exercise group showed significant increase in the %LBM and LBM(p<.001).
Effects of Houttuynia Cordata thunb on the liver damage of TCDD-treated rats
Journal of Life Science, volume 13, issue 4, 2003, Pages 457~462
DOI : 10.5352/JLS.2003.13.4.457
Houttuynia Cordata thunb has been used as folk medicine for analgesics, beriberi, edema, hepatitis and icterus etc. We investigated, the effects of Houttuynia Cordata thunb administration on protective in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) treated rats. Seven days after the injection of TCDD(1
/kg), Houttuynia Cordata thunb (200mg/kg) was administered into rats intraperitoneally for four weeks. We examined the antioxidative enzymatic activity by measuring the level of GOT, GPT in serum and MDA, GSH, GSSG, GPx, SOD and Catalase in liver tissue of rats. GOT activity of Houttuynia Cordata thunb and TCDD administered group(HTT) showed 49.00% of inhibitive effect compared to TCDD-treated abnormal group(TTA). GPT level of HTT group was decreased to the level of Non TCDD-treated group(NTT). MDA content in the TTA group was 1.27 times increased compared to NTT group. HTT group was inhibited by 69.53% compared to TTA group. GSH contents in HTT group was 1.91 times increased compared to TTA group. GSSG contents in HTT group was 46.72% decreased compared to TTA group. SOD and Catalase in TTA group were lower than in NTT group, but SOD and Catalase in HTT group were increased by 82% and 55.45% respectively compared to TTA group.
Characterization of tryptophan residues of human coagulation factor V required for binding to phospholipid membranes
Kim, Suhng-Wook ;
Journal of Life Science, volume 13, issue 4, 2003, Pages 463~472
DOI : 10.5352/JLS.2003.13.4.463
Interactions between factor Va (HFVa) and membrane phosphatidylserine (PS) regulate the activity of the prothrombinase complex. I have previously shown that two solvent exposed hydrophobic residues located in the C2-domain, Trp2063 and Trp2064, are required for binding to immobilized PS and for expression of procoagulant activity on membranes containing 5% PS. In order to fully define the functional importance of these two residues I have expressed and isolated recombinant factor Va (rHFVa) W2063A/W2064A double mutant. In contrast to the native protein the two glycoforms resulting from alternative glycosylation of Asn2181 eluted as a single peak with rHFVa1 W2063A/W2064A eluting on the leading edge and rHFVa2 W2063A/W2064A eluting on the trailing edge. The double mutant rHFVa2 W2063A/W2064A expressed little or no procoagulant activity on membranes containing 1-10% mol % PS. In contrast, the procoagulant activity of this mutant was slightly greater than the native protein on membranes containing>18 mol % PS. The binding of rHFVa2 W2063A/W2064A to immobilized phospholipid vesicles was markedly reduced compared to the native protein in a surface plasmon resonance binding assay. I conclude that Trp2063 and Trp2064 are required for high affinity binding of factor Va to PS membranes and that this interaction is necessary for assembly of the prothrombinase complex on membranes containing physiological concentrations of PS.
Comparison of Patterns of Mineral Ions and Growth Responses of 4 Legume Plants by Nitrogen Applications under Saline Conditions
Journal of Life Science, volume 13, issue 4, 2003, Pages 473~480
DOI : 10.5352/JLS.2003.13.4.473
We analyzed the patterns of mineral ions and growth responses among symbiotic nitrogen fixing legumes by external nitrogen applications under salt gradients. Glycine max, Phaseolus angularis and Albizzia julibrissin showed remarkable growth inhibition above 40 mM NaCl treatments, but Cassia tora did not exhibit any visible injury symptom up to 100 mM NaCl treatments. As to ionic pattern, the
contents in leaves of G. max, P. angularis and A. julibrissin progressively increased with higher contents of external salinity. Compared to other plants, C. tora excluded
more efficiently and maintained rather constant ionic contents in spite of high salt levels. With a few exception, these 4 legume plants exhibited better growth by the external nitrogen supply rather than the contribution of symbiotic nitrogen fixation only under saline condition.
Effects of Heating Condition and Additives on Rheology of Squid Meat Paste Products
Journal of Life Science, volume 13, issue 4, 2003, Pages 481~491
DOI : 10.5352/JLS.2003.13.4.481
A squid meat has not been utilized for gel products because of its lower gel forming ability. The objectives of this study were as followed; 1) the optimum heating condition on squid meat paste products and 2) the optimum added level for jelly strength of squid meat paste products. Optimum heating conditions of squid meat kamaboko were as followed; setting (pre-heating) at 15
for 2 hours and heating at 90
for 60 minutes. Effect for jelly strength of starch additives wheat starch, potato starch and com starch were examined. The jelly strength of heat induced gels differed from the levels of additives. In case of adding starch, potato starch was resulted in the superior jelly strength than the other starchs, wheat starch and corn starch, at any levels. Optimum concentration was 10%(w/w) at every additives. Folding test value was B at added 10% and this value was mean good product. Data of jumbo and flying squid meat paste products added potato starch, corn starch and wheat starch of 10% were shown below, jelly strengths were 858
37gㆍcm and 966
45gㆍcm and moisture contents were 72.43∼73.04% and 71.61∼72.78%, respectively. To adding edible agar and sea tangle, showed the highest jelly strength (edible agar>sea tangle, flying squid>jumbo squid) at added 0.5%(w/w) concentration.
A study on the Mycelial Growth of Lentinus lepideus in Liquid Culture
Journal of Life Science, volume 13, issue 4, 2003, Pages 492~497
DOI : 10.5352/JLS.2003.13.4.492
This study was carried out to get the basic data for the mycelial growth of Lentinus lepideus in liquid culture. The optimal temperature and initial pH of mycelial growth of Lentinus lepideus were
and pH 5.5, respectively. The optimal medium was YMG medium. Among the carbon sources tested, glucose was effected to the mycelial growth and optimal glucose concentration was 4% (w/v). As nitrogen sources, malt extract and yeast extract appeared to be favorable and optimal malt extract and yeast extract [ratio (w/w) of 1:1] concentration was 1.5% (w/v).
A Study on the Cultural Characteristics of Pholiota nameko Mycelium
Journal of Life Science, volume 13, issue 4, 2003, Pages 498~504
DOI : 10.5352/JLS.2003.13.4.498
This Study was carried out to investigate the optimal mycelial growth of Pholiota nameko. The optimal medium for the mycelial growth was ME medium. The optimal temperature and pH were
1 and 5.5, respectively. The modified optimal medium compositions were glucose 3% (w/v), malt extract 0.25% (w/v), yeast extract 0.25% (w/v),
0.05% (w/v). From the result of experiments on the optimal temperature, pH and nutritional requirements, the mycelial growth of modified optimal medium was higher than that of ME medium.
Isolation of Bacteriophage from Haloarcular sp, EH-1
Journal of Life Science, volume 13, issue 4, 2003, Pages 505~510
DOI : 10.5352/JLS.2003.13.4.505
The extremely halophilic archaebacteriurn Haloarcular sp. EH-1 was isolated from solar salts. Halophages found in Haloarcular sp. EH-1 were isolated from fermented anchovy sauce. Halophages were isolated from fermented anchovy sauce using Haloarcular sp. EH-1 as a host bacterium. The isolated halophage produced 0.5∼l.0 mm in diameter clear plaques. The halophage consists of an symmetrical head, measuring 68 nm in diameter, and a contractile tail, 100 nm long and base plates were observed. Total size of phage DNA genome obtained 20 Kbp and its sequence homology was 52.87% with H. Salinarium.
Characterization of Aerobic Decolorization of Remazol Black B by White Rot Fungi
Journal of Life Science, volume 13, issue 4, 2003, Pages 511~515
DOI : 10.5352/JLS.2003.13.4.511
White rot fungi, Phanerochaete sp. EJ-31L, was evaluated for its ability to decolorize Remazol Black B, an azo dye that is a widespread pollutant in the wastewater of textile industry. It was observed that extent of decolorization by Phanerochaete sp. EJ-31L was dependent on the concentrations of co-carbon and nitrogen source. Effects of agitation and aeration were studied, and agitated culture at aeration condition resulted in greater extent of decolorization than static culture. Remazol Black B was readily decolorized up to 95% within 64 hr by Phanerochaete sp. EJ-31L.
Isolation of protoplast from the marine red alga Porphyra pseudolinearis in Korea
Journal of Life Science, volume 13, issue 4, 2003, Pages 516~521
DOI : 10.5352/JLS.2003.13.4.516
Optimal conditions of protoplast isolation from the seaweed Prophyra pseudolinearis have been described. The P. pseudolinearis is one of indigenous and dominant Porphyra species in the East Sea region. Protoplasts have been released by enzymatic treatment of 2% agarase and 2% hemicellulase in 25mM MES buffer, pH 6.0 containing 0.5 M sorbitol. The protoplasts could be fused with neutral red-stained protoplasts of P. okamurae by the addition of polyethylene glycol 8000 solution.
Characterization of RAD4 Homologous Gene from Coprinus cinereus
Choi, In-Soon ;
Journal of Life Science, volume 13, issue 4, 2003, Pages 522~528
DOI : 10.5352/JLS.2003.13.4.522
The RAD4 gene of Saccharomyces cerevisiae is essential for the incision step of UV-induced excision repair. A yeast RAD4 gene has been previously isolated by functional complementation. In order to identify the RAD4 homologous gene from fungus Coprinus cinereus, we have constructed cosmid libraries from electrophoretically separated chromosomes of the C. cinereus. The 13 C. cinereus chromosomes were resolved by pulse-field gel electrophoresis, hybridized with S. cerevisiae RAD4 DNA, and then isolated homologous C. cinereus chromosome. The insert DNA of the RAD4 homolog was contained 3.2 kb. Here, we report the characterization of fungus C. cinereus homolog of yeast RAD4 gene. Southern blot analysis confirmed that C. cinereus contains the RAD4 homolog gene and this gene exists as a single copy in C. cinereus genome. When total RNA isolated from C. cinereus cells was hybridized with the 1.2 kb PvuII DNA fragment of the S. cerevisiae RAD4 gene, a 2.5 kb of transcript was detected. In order to investigation whether the increase of transcripts by DNA damaging agent, transcripts levels were examined after treating the cells. The level of transcript did not increase by untraviolet light (UV). This result indicated that the RAD4 homologous gene is not UV inducible gene. Gene deletion experiments indicate that the RAD4 homologous gene is essential for cell viability.
Sterol Composition and Phytoestrogen Activity of Safflower(Carthamus tinctorius L.) Seed
Journal of Life Science, volume 13, issue 4, 2003, Pages 529~534
DOI : 10.5352/JLS.2003.13.4.529
This study was done to investigated the phytosterol compositions of safflower (Carthamus tinctorius L.) seed. The phytoestrogen activity was also determined using CAT-ELISA Kit in ethanol extract of safflower seed. The phytosterol of safflower seeds was identified using gas chromatography-mass spectrometry after saponification of the oils. The phytosterol content and composition of safflower seed oils were 4% and identified stigmast-5-en-3-ol (3
-sitosterol (clionasterol) with Wiley MS spectrum library. The synergistic effect of human estrogen receptor (hER) has been investigated using a minimal chimeric promoters composed of the TATA region of the adenovirus-2 major late promoter (A22MLP) and two consensus perfectly polindromic Xenopus vitellogenin A2 gene estrogen responsive elements (XVEREl19). Transient transfection experiments in tile human breast adenocarcinoma cell line MCF-7, which is known to express the estrogen receptor endogenously, revealed that phytoestrogen from Carthamus tinctorius L. acts as estrogen. We have observed the transcriptional activities stimulated methanol and ethanol extract of safflower seed in MCF-7, were 0.43 and 0.37 respectively, compared to that by
-estradiol as 1.0. Our data showed that safflower seeds have estrogenic activity methanol and ethanol extracts and ethanol lower than that of
-estradiol. This result provides the first evidence that the beneficial effect of safflower seeds may be mediated, at least in part, by the stimulating effect of phytoestrogen ell bone-protecting.
Isolation and Identification of the Antagonistic Microorganisms Against Streptococous spp. Causing Dental Caries in Korean Soy Sauce
Journal of Life Science, volume 13, issue 4, 2003, Pages 535~540
DOI : 10.5352/JLS.2003.13.4.535
The antagonistic microorganisms against Streptococcus sanguis, S. salivarius and S. mutans causing the dental caries of oral diseases were isolated from Korean traditional soy sauce. Twenty five strains were isolated by pairing culture, paper disc culture and dual culture methods. The isolate NG 06 strain was observed with various cultural and physiological test, and
Bacterial Identification System. The strain was identified as Bacillus racemilacticus. The isolate NG 16 strain was confirmed to Gram-positive, rods, endospore production, utilization of melibiose, casein hydrolysis and starch hydrolysis. Also the second strain NG 16 was identified as
Cloning of cDNA Encoding Putative Cellular Receptor Interacting with E2 protein of Hepatitis C Virus
Journal of Life Science, volume 13, issue 4, 2003, Pages 541~550
DOI : 10.5352/JLS.2003.13.4.541
E2 glycoprotein of hepatitis C virus (HCV) comprises a surface of viral particle together with E1 glycoprotein, and is thought to be involved in the attachment of HCV viral particle to receptor (s) on the permissible cells including hepatocytes, B cells, T cells, and monocytes. We constructed a phage library expressing cellular proteins of hepatocytes on the phage surface, which turned out to be 8.8
cfu of diversity and carried inserts in 95% of library. We screened both cDNA phage library and 12-mer peptide library to identify the cellular proteins binding to E2 protein. Some intracellular proteins including tensin and membrane band 4.1 which are involved in signal transduction of survival and cytoskeleton organization, were selected from cDNA phage library through several rounds of panning and screening. On the contrary, membrane proteins such as CCR7, CKR-L2, and insulin-like growth factor-1 receptor were identified through screening of peptide library. Phages expressing peptides corresponding to those membrane proteins were bound to E2 protein specifically as determined by neutralization of binding assay. Since it is well known that HCV can infect T cells as well as hepatocytes, we examined to see if E2 protein can bind to CCR7, a member of C-protein coupled receptor family expressed on T cells, using CCR7 transfected tells. Human CCR7 cDNA was cloned into pcDNA3.1(-) vector and transfected into human embryonic kidney cell, 293T, and expressed on the surface of the cell as shown by flow cytometer. Binding assay of E2 protein using CCR7 transfected cells indicated that E2 protein bound to CCR7 by dose-dependent mode, giving rise to the possibility that CCR7 might be a putative cellular receptor for HCV.