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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 14, Issue 6 - Dec 2004
Volume 14, Issue 5 - Oct 2004
Volume 14, Issue 4 - Aug 2004
Volume 14, Issue 3 - Jun 2004
Volume 14, Issue 2 - Apr 2004
Volume 14, Issue 1 - Feb 2004
Selecting the target year
Stuy on the Composition of Pleurotus ferulae Fruit Body
Journal of Life Science, volume 14, issue 2, 2004, Pages 205~208
DOI : 10.5352/JLS.2004.14.2.205
The composition of Pleurotus ferulae fruit body (PFF) has been investigated to determine the possible use for medicinal and edible purposes. The nutritional compositions of PFF were as follows; carbohydrate 54.3 g%, crude protein 20.2 g%, moisture 12.5 g%, crude fat 8.0 g% and ash 5.0 g% (w/w). Potassium concentration in PFF was high up to 2,337.0 mg% (w/w) and Mg, Na, Zn, Ca and Fe were followed. There were seventeen amino acids in PFF. The glutamic acid content was high up to 13.65% mol and aspartic acid, glysine, alanine, leucine, arginine, valine and lysine were followed. Since PFF contains major free amino acids such as alanine, phenylalanine, tyrosine and leucine, it will be a favorable food stuff, Vitamin contents of PFF were vitamin A 0.12, vitamin B
0.31, vitamin B
0.68, vitamin C 7.99, vitamin E 316.88 and vitamin D
0.29 mg%. PFF will have a good anti-aging effect due to the high content of vitamin C and E.
Effects of Chitosan on Anti-tumor Activity in Mice
Journal of Life Science, volume 14, issue 2, 2004, Pages 209~214
DOI : 10.5352/JLS.2004.14.2.209
Cytotoxic anticancer chemotherapeutic agents generally produce severe side effects, while reducing host resistance to cancer and infections, especially through the destruction of lymphoid and bone marrow cells. In this study, we have investigated the effect of chitosan on cytotoxic activity against cancer cells and life span in mice. The direct cytotoxicity of chitosan or chitosan-combinated chemotherapeutic agents for tumor cells was observed. In addition, the effect of life span extention was counted on sarcoma 180 mice injected with chitosan-combinated mitomycin C. The effect of growth inhibion for cancer cells, K562 and Yac-1 was shown in the cytotoxicity test of chitosan or chitosan-combinated chemotherapeutic agents. Also, the effect of life span extension was observed on sarcoma 180 mice injected with chitosan-combinated mitomycin C. Our results suggest that life span extension in sarcom 180 mice exposed with chitosan-combinated chemotherapeutic agents showed the probability of its usefulness for cancer therapy if more research results were accumulated.
The Serum and Immunohistochemical Analysis on the Anti-infammatory Effect of Aqueous Extract from Artemisia capillaris in the Liver of Lipopolysaccharide-injected Rat
Journal of Life Science, volume 14, issue 2, 2004, Pages 215~220
DOI : 10.5352/JLS.2004.14.2.215
We investigated the anti-inflammatory effects of aqueous extract of Artemisia capillaris Thunb. (AEAC), a traditional Korean herb for remedying liver disease, for suppression in the process of lipopolysaccharide (LPS)-induced inflammation in the liver of rat. Level of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) was increased in the serum of LPS-treated rats compared to normal, however, in the rats pretreated with AEAC, the increase of GOT, GPT and LDH value was arrested. More severe histological changes of liver such as cloudy swelling, hydropic degeneration, Kupffer cell reaction and inflammatory cells infiltration were demonstrated in the rats challenged with LPS compared with normal. Fewer scores of these changes were observed in rats pretreated with AEAC. Immunohistochemical analysis showed that while the expression of the nuclear factor (NF)-kBp65, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-
and COX (cyclooxygenase)-2 tended to increase, that of inhibitory (I)-kBa was decreased in the hepatocytes of rats challenged with LPS. A slight decline of NF-kBp65, TNF-
and COX-2, but increase of I-kB
were observed in the hepatocytes of the rats pretreated with AEAC. These results suggest that AEAC may act as a therapeutic agent for liver disease through a regulation of inflammation-related proteins.
Quantitative Analysis Cholesterol in Each Parts of Korean Squid by the Chromarod TLC-FID System(Iatroscan)
Journal of Life Science, volume 14, issue 2, 2004, Pages 221~224
DOI : 10.5352/JLS.2004.14.2.221
Comparisons of enzymatic method, gas chromatographic method, and the Iatroscan method for the determination of cholesterol in each parts of Korean squid were undertaken. The Iatroscan method was the most suitable procedure for the rapid and simple determination of net cholesterol concentration in parts of Korean squid. 5
-cholestane is used as a good internal standard. The cholesterol contents in body, leg, viscera, eye, skin, and liver part of Korean squid, Todarodes pacificus STEEN STRUP by Iatroscan method were 178.9, 321.4, 168.9, 159.5, 608.8 and 634.2 mg%, respectively.
Development on the Purification Process of Natamycin from Streptomyces natalensis ATCC27448
Journal of Life Science, volume 14, issue 2, 2004, Pages 225~228
DOI : 10.5352/JLS.2004.14.2.225
Natamycin, produced by Streptomyces natalensis ATCC27448, is a polyene macrolide antibiotic, is widely used in the food industry in order to prevent mould contamination. This study carried out to develop an efficient purification process of natamycin from fermentation broth. The stability of natamycin in fermentation broth during storage period was investigated at 4
and room temperature. After the storage of fermentation broth for 14 days at 4
, residual activity of natamycin was about 80% but decreased by 27% at room temperature. As solvent to extract natamycin from fermentation broth, methanol was the most efficient. A developed purification procedure includes methanol extraction and Diaion HP-20 column chromatography. Approximately 2.9 g of natamycin was obtained with a final yield of 69.1% and purity of 96.6% from 1.8 l of fermentation broth by this developed purification procedure.
Isolation and Identification of Feather-Degrading Bacteria for Biotechnological Applications of Keratinaceous Protein Waste
Journal of Life Science, volume 14, issue 2, 2004, Pages 229~234
DOI : 10.5352/JLS.2004.14.2.229
Feathers, which are almost pure keratin protein, are produced in large amounts as a waste by-product at poultry-processing plants. Keratinolytic enzymes may have important uses in biotechnological processes involving keratin-containing wastes from poultry and leather processes. In this study, screening and identification of keratin-degrading bacteria were investigated. Five keratin-degrading bacterial strains (F3-1, F3-4, F7-1, C1-1, C1-2) were isolated from compost and decayed chicken feather. On the basis of morphological, physiological studies, and Biolog system, all isolates were identified as the genus Bacillus. Among them, the strain F7-1 had the highest feather-degrading activity and was selected for further taxonomical study. Phylogenetic analysis of strain F7-1 based on comparison of 165 rDNA sequences revealed that this strain is closely related to Bacillus megaterium.
Possibility to Develope the Multistage Culture System for Larvae Cultivation by Computer Simulation
Journal of Life Science, volume 14, issue 2, 2004, Pages 235~238
DOI : 10.5352/JLS.2004.14.2.235
The possibility for developing multistage culture system to cultivate larvae by computer simulation with basic experiments was investigated. This culture system was composed of 3 stages. At the 1st stage, Chlorella sp. were cultivated and at 2nd stage Chlorella sp. were supplied to rotifer (Brachionus plicatilis), and rotifer were supplied to larvae at the 3rd stage. In this study, Chlorella sp. were cultivated by batch culture to search for the possibility of continuous feeding to rotifer at 2 nd stage. The maximum specific growth rate (
) of Chlorella sp. at the logarithmic phase was 0.56 [1/day]. Rotifer was cultivated by fed-batch culture at the feeding rate of Chlorella sp.,
[cells/rotiferㆍh] to search for the relation between the feeding rate of Chlorella sp. and the growth rate of rotifer. As the results, the minimum feeding rate of Chlorella sp. was 2.8
[cells/rotiferㆍday] in the multistage culture system, then the change of rotifer concentration at 2nd stage was simulated by computer. The required amount of rotifer for the growth of larvae was also increased as the growth of larvae. On the 9th day of the culture, the rotifer uptake rate of larvae was 250 [cells/rotiferㆍday]. Based on these basic experiments and results, It was suggested that the possibility of multistage culture system to cultivate larvae with continuous feeding of Chlorella sp. and rotifer.r.
Effect of Carcass Grade and Addition of Mugwort on the Physicochemical Properties and Palatability of Loin Ham
Journal of Life Science, volume 14, issue 2, 2004, Pages 239~244
DOI : 10.5352/JLS.2004.14.2.239
This study was carried out to clarify the effect of addition of mugwort and carcass grade on the quality and sensory properties of loin hams. The volatile basic nitrogen, 2-thiobarbituric acid reactive substances, total bacterial count, water holding capacity and calorie of loin hams were not significantly different among hams, but the pH and residual nitrite of loin hams with mugwort were significantly lower than that of loin hams without mugwort. The fat content of grade B loin hams were higher than that of grade E loin hams, and the protein and total amino acid content of grade E loin hams were higher than that of grade B loin hams. The free amino acid, saturated fatty acid and unsaturated fatty acid were not significantly different among loin hams. The
value of grade B loin hams were higher than that of grade E loin hams, the a value of grade E loin hams were higher than that of grade B loin hams, and the
value was not significantly different among loin hams. The hardness, springiness, cohesiveness, gumminess and chewiness were not significantly different among loin hams. The aroma of loin hams without mugwort were superior than that of loin hams with mugwort, and the juiciness of grade B loin hams were superior than that of grade E loin hams. But the color, taste, texture and palatability were not significantly different among loin hams.
The Effect of Low-Intensity Treadmill Exercise on Blood Levels of Glucose, Insulin and Lipids in Streptozotocin-Induced Diabetic Rats
Journal of Life Science, volume 14, issue 2, 2004, Pages 245~251
DOI : 10.5352/JLS.2004.14.2.245
Exercise is beneficial to the diabetic patients and streptozotocin-induced diabetic rat has been used for the study of exercise effect. The purpose of this study was to establish the optimal condition of induction of hyperglycemic diabetic rat using streptozotocin and to examine the preventive effect of treadmill exercise on the diabetic rat before and after streptozotocin injection. Intraperitoneal injection of increasing amount of streptozotocin up to 40 mg/kg dose-responsively induce hyperglycemic diabetic rat and inversely reduced the blood insulin level. Body weight was also gradually reduced with the increasing amount of streptozotocin. Control and diabetic rats exercised for 4 weeks before streptozotocin injection. The exercise was performed in the treadmill for 25 minutes a day and 5 times a week with low intensity (0 degree tilt, 15 m/min velocity). Following streptozotocin injection, the blood glucose level was measured every week and the rat was sacrificed after 4 weeks to measure the concentration of insulin and blood lipids. The blood levels of glucose and insulin was significantly reduced with exercise before streptozotocin injection, while those were not changed after streptozotocin injection. The levels of blood lipids such as total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride were close to normal control rats. From this study, researchers found the optimal condition of preparation of streptozotocin-induced hyperglycemic diabetic rat, and the mild treadmill exercise has beneficial effect on preventing hyperglycemia and hyperlipidemia. Thus, even low intensive running prevent not only diabetes but also diabetic vascular complications.
Analysis of Mutant Tryptophan Synthases with Defective Enzymatic Properties
Journal of Life Science, volume 14, issue 2, 2004, Pages 252~254
DOI : 10.5352/JLS.2004.14.2.252
The mutant tryptophan synthases at
have low catalytic activities. The enzymes were treated with
subunit-specific ligands in the presence of L-serine and indoline. It was shown that the cations resulted in changes of absorbance patterns among the proteins, while glycerophosphate showed similar pattern of absorbance. The glycerophosphate binds to the active site of
subunit so that
may not be involved in the allosteric control with the active site of
subunit occupied by substrate. The results suggest that
may playa role in the step of a series of reaction occurring without bound substrates in the active site of asubunit.
Application of DNA Content and Total Protein Concentration to Predict Blooms Caused by Cochlodinium polykrikoides (Dinophyceae) in Korean Coastal Waters
Cho, Eun-Seob ; Park, Yong-Kyu ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 255~262
DOI : 10.5352/JLS.2004.14.2.255
We applied nuclear DNA content stained with 4'-6'-diamidino-2-phenylindole (DAPI) and total protein concentration to predict the existence of Cochlodinium polykrikoides before huge blooms occurred, based on a short-term survey at sites in the South Sea. Fluctuations in environmental conditions and nutrients (nitrate, nitrite, and phosphate) were of a similar range, regardless of sampling sites or early and middle field observations. However, C. polykrikoides abundance was significantly different depending on the station, with a higher cell density of 34, 62, and 57 cells L
at Stn C2, C5, and C6, respectively than what was found in early August, 2000. In mid August, 2000, the highest cell density of 547 cells L
at Stn C3 was observed. The relationship between C. polykrikoides abundance, DAPI-stained DNA content, and total protein concentration was a positive correlation coefficient, in particular a higher positive correlation was exposed to even a smaller abundance of C. polykrikoides. These results suggest that DNA stained by DAPI and total protein concentration could play an important index in easily predicting the presence of C. polykrikoides before blooms.
Effect of Fibroblast Growth Factor-2 on the Sprouting in Vascular Endothelial Cells
Journal of Life Science, volume 14, issue 2, 2004, Pages 263~268
DOI : 10.5352/JLS.2004.14.2.263
The sprouting of vascular endothelial cells is an initial step in angiogenesis. Matrix metalloproteinases can associate with integrin on the surface of endothelial cells, thereby promoting angiogenesis. The purpose of this study was to test if fibroblast growth factor-2 (FGF-2) can regulate the sprouting in porcine pulmonary artery endothelial cells. FGF-2 induced sprouting and secretion of MMP-2 and plasmin. FGF-2 also induced the expression of integrin Mac-1, which is inhibited IS20I. Addition of BB-94, a 2-antiplasmin and IS20I inhibited FGF-2-induced sprouting activity. Therefore, FGF-2-induced sprouting activity in PPAECs may be accomplished by secretion of proteinases such as MMP-2 and plasmin and integrin expression.
Esterification Reaction of Soybean Oil by Heterogeneous Catalysts
Journal of Life Science, volume 14, issue 2, 2004, Pages 269~274
DOI : 10.5352/JLS.2004.14.2.269
Using heterogeneous catalyst, esterification reaction of soybean oil (SBO) with methanol was investigated. Distributions of components in mixtures of soybean oil and methanol were measured at temperatures ranging from 40 to
. Glycerine contents of reaction mixtures were measured for the different kinds of catalysts, such as NaOH, CaO, Ca(OH)
, MgO, Mg(OH)
, and Ba(OH)
. Based on the measured glycerine concentrations, conversions of the reaction mixtures were calculated. The effects of dose of catalyst, cosolvent and reaction temperature on final conversion were examined. Solubility of methanol in soybean oil was substantially greater than that of soybean oil in methanol. When the esterification reaction of soybean oil was catalyzed by heterogeneous catalyst, final conversion was strongly dependent on the alkalinity of the heterogeneous catalyst, and increased with the alkalinity of the catalyst material. Hydroxides from the alkali metals were more effective than oxides, which actually had no catalytic effects. When Ca(OH)
was used for the esterification catalyst, maximum value of final conversion was measured at dose of 4%. The final conversion and reaction rate increased with reaction temperature, and showed substantial increment at reaction temperature of 5
. When cosolvent, CHCl
, was added into the reaction mixture of soybean oil which catalyzed by Ba(OH)
, maximum value of final conversion was appeared at dose of 3%.
Novel Variations in Human Interleukin-29 and Their Association
Song, Ju-Hee ; Chae, Soo-Cheon ; Lee, Jae-Hoon ; Chung, Hun-Taeg ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 275~279
DOI : 10.5352/JLS.2004.14.2.275
Gene polymorphisms of cytokines and their receptors are attractive candidates as genetic factors in the pathogenesis of immune-mediated diseases and have been reported to be associated with disease susceptibility to autoimmune, inflammatory and infectious diseases. IL-29 is one of important candidate genes for complex trait of genetic diseases but there is no published survey of single nucleotide polymorphisms (SNPs) in this gene. In this study, for the first time, we have examined the full genomic sequence of IL-29 including the promoter regions to identify SNPs. We examined the frequencies of genotypes and alleles at the SNP site of IL-29 in allergic rhinitis patients and non-allergic rhinitis controls using the direct sequencing method to determine whether this IL-29 SNP is associated with allergic rhinitis in Korean population. We identified one novel SNP (1184C＞A) in the intron 2 and one novel variation site (-1842_-1841dupGA) in the promoter region of human IL-29 gene. The P values of SNP or variation site were not significant between the healthy controls and allergic rhinitis patients. Our results suggest that the 1184C＞A polymorphism and -1842_-1841dupGA variation site in human IL-29 gene were not associated to allergic rhinitis.
Reduction of Oxidative Stress by Chondroitin Sulfate in the Ovariectomy-Induced Aging Rat
Journal of Life Science, volume 14, issue 2, 2004, Pages 280~285
DOI : 10.5352/JLS.2004.14.2.280
The ovarian hormone-deficiency induced ovariectomy rat is widely used as an aging model due to its practicality, convenience, and cost effectiveness. The surgically ovariectomized rat induces reactive oxygen species (ROS) generation like aging phenomena. Free oxygen radicals have been proposed as important causative agents of aging. The purpose of this study was to investigate the effect of chondroitin sulfate (CS) to prevent ovariectomy (OVX)-induced oxidative stress. The OVX rats were given intraperitoneally CS at doses of 100 mg/kg and 200 mg/kg daily for fifteen weeks. Malondialdehyde (MDA) levels were determined as well as the activities of superoxide dismutase (SOD), catalase (CAT), reduced-glutathione (GSH), oxidized-glutathione (GSSG), glutathione peroxidase (GPx) in the liver. The liver antioxidative enzyme activity was elevated while MDA concentration decreased in all CS treated animals. The results demonstrated that CS reduced oxidative stress in a dose dependent manner. These results suggest that CS might be a useful candidate for antioxidative reagent.
Antioxidant Activity of Various Fractions Extracted from Mustard Leaf (Brassica juncea) and Their Kimchi
Kim, Jae-I ; Park, Jae-Sue ; Kim, Woo-Seong ; Woo, Kang-Lyung ; Jeon, Jung-Tae ; Min, Byung-Tae ; Cheigh, Hong-Sik ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 286~290
DOI : 10.5352/JLS.2004.14.2.286
The antioxidant activities of the various fractions extracted from mustard leaf (Brassica juncea) and mustard leaf kimchi were determined by the radical scavenging effect on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical. The fractions from dried mustard leaf, and fermented mustard leaf kimchi for 0 day and fermented mustard leaf kimchi for 5 days at 15
were screened for the scavenging effects by using DPPH assay. The fractions prepared by the systematic extraction procedure with the solvents such as hexane, C
, EtOAc, BuOH and
were used for the determination of free radical scavenging effects. The antioxidant activity of EtOAc and n-BuOH soluble fraction from mustard leaf and mustard leaf kimchi for 0 day had stronger than the others. During fermentation at 15
for 5 days, the antioxidant activity was changed. C
and EtOAc soluble fraction showed more potent radical scavenger effects than the others. The difference or results were to the various supplements and fermentation process.
Neuronal Protection by Rooibos (Aspalathus linearis) Tea Infusions in a Hypoxic Model of Cultured Rat Cortical Neurons
Moon, Il-Soo ; Ko, Bok-Hyun ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 291~295
DOI : 10.5352/JLS.2004.14.2.291
Rooibos (Aspalathus linearis) (RB) is a leguminous shrub native to the mountainous areas of the northwestern Cape Province in South Africa. RB tea infusions are the fermentation products of its leaves and fine sterns, and known to have a high antioxidative activity due to the presence of flavonoids and phenolic acids. We investigated the effects of RB tea on the alleviation of oxidative stress on cultured rat cortical neurons in a hypoxic model. Measurement of lactate dehydrogenase (LDH) released into culture media revealed that RB increased cell viabilities in both normoxia (6-18%) and hypoxia (2-24%) dose-dependently (10-100
/ml) on 16 days in vitro (3 days after treatment). Visualization of cell morphology by expression of GFP-Hsc70 fusion protein showed that RB (50
/ml) reduced the average vacuolated soma from 55.4
4.59% (no RB addition) to 40.9
6.3% (RB addition) on 5 days after hypoxia. Our results proves efficacy of RB in the neuroprotection of hypoxic neurons and extend application for RB into the prevention and/or treatment of neuronal damages.
Identification of a Mitochondrial DNA Mutation in Paraffin-Embedded Muscle Tissues
Journal of Life Science, volume 14, issue 2, 2004, Pages 296~300
DOI : 10.5352/JLS.2004.14.2.296
We investigated feasibility of using the formalin-fixed and paraffin-embedded tissue to study mitochondrial mutations in the case that fresh or frozen tissue, or blood samples are not available. Four paraffin blocks of muscle biopsies in Korean MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes) patients were chosen. Total DNA was extracted from these blocks for PCR/RFLP analysis, and sequencing was performed to study the most common mutation, A to G transition at nucleotide position 3243 underlying MELAS in the mitochondrial tRN
gene. We could identify the A to G mutation at nt.3243 in three MELAS patients. Our results show that the mitochondrial genome of our paraffin blocks is presumably in good condition. Our results are in accordance with the previous findings by other investigators that PCR allows molecular genetic analysis of paraffin-embedded tissues stored in most histopathology laboratories.s.
Species Identification of Nontoxic Alexandrium tamarense (Dinophyceae) from Chinhae Bay, Korea, Using Molecular Probes
Cho, Eun-Seob ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 301~308
DOI : 10.5352/JLS.2004.14.2.301
The morphology of the apical pore complex, the first apical plate and the posterior sulcal plates in a new isolate of Alexandrium tamarense (Lebour) Balech from the Bay of Chinhae was compared with other that of toxic strains of A. tamarense previously isolated from Korean waters. Although this isolate was morphologically identical to these toxic strains, high performance liquid chromatography and mouse bioassay showed no evidence of toxin production. The nontoxic A. tamarense strain showed a strong positive binding activity with PNA lectin, indicating a high density of lactose and galactose residues on the cell surface, and in SDS-PAGE and Western blot analysis a unique protein of about 21-kDa molecular sizes was observed. These findings demonstrate that the use of PNA and immunobioassay could be used to discriminate between toxic and nontoxic strains of A. tamarense.
Application of Temperature Gradient Gel Electrophoresis To cAMP Receptor Protein
Gang, Jong-Back ; Cho, Hyun-Young ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 309~314
DOI : 10.5352/JLS.2004.14.2.309
Cyclic AMP receptor protein (CRP) is involved in the transcriptional regulation of more than 100 genes in E. coli. CRP dimer is converted into active form via the sequential conformation change of cAMP binding pocket, hinge region and HTH DNA binding motif by binding of cAMP. The temperature gradient gel electrophoresis (TGGE) was applied to CRP protein to know whether it was an efficient technique to study the conformational transitions and the thermal stability. TGGE showed the unfolding process of wild-type and S83G CRP proteins with the temperature gradient set from 29 to 71
on nondenaturing polyacrylamide gel. Melting temperature (Tm) was 57
1 and 55
for wild-type and S83G CRP, respectively in acidic buffer[89.8 mM Glycine and 24 mM Boric acid (pH 5.8)].
Expression of Human Serine Palmitoyltransferase Genes for Antibody Development
Journal of Life Science, volume 14, issue 2, 2004, Pages 315~319
DOI : 10.5352/JLS.2004.14.2.315
For antibody development of human serine palmitoyltransferase (SPT, EC 220.127.116.11), SPTLC1 and SPTLC2 genes were subcloned in pRset vector and expressed in E. coli BL21 (DE3)pLys cells. Eucaryotic SPT is a membrane-bound heterodimer enzyme, while all other members are soluble homodimer enzymes. cDNA library were obtained from total RNA from human embryo kidney cell line, HEK293, using RT-PCR and PCR with specific primers was carried out for preparing SPTLC1 and SPTLC2 genes. pRset vector which can express hexahistidine-tag fusion protein was used and the DNA sequences of pRsetB/SPTLC1 and pRsetA/SPTLC2 were confirmed. Recombinant BL21 cells with SPTLC subunits were selected with LB plate containing ampicillin and chroramphenicol. SPTLC1 and SPTLC2 proteins were induced with 1 mM IPTG and seperated on 10% SDS-PAGE gel. Expressed proteins were confirmed by western blotting with His-tag antibody.
Isolation and Characteristics of Biosurfactant Producing Bacterium, Bacillus sp. TBM 911-5
Journal of Life Science, volume 14, issue 2, 2004, Pages 320~324
DOI : 10.5352/JLS.2004.14.2.320
The objective of this study was investigate the characteristic of biosurfactant produced from the isolated strain. The strain was isolated from soil samples and identified as Bacillus sp. TBM 911-5 by physiological characteristics and the partial nucleotide sequence analysis of 16S rDNA. We measured the surface tension every 6 hours for 80 hours. The surface tension of the culture filtrate of Bacillus sp. TBM 911-5 was decreased to 29 mN/m. Biosurfactant concentration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The biosurfactant emulsified hydrocarbons, vegetable oil and crude oil. Using soybean oil as substrate, the maximum emulsification activity and stability was obtained from the biosurfactant. The biosurfactant produced from Bacillus sp. TBM 911-5 had strong properties as an emulsifying agent and an emulsion-stabilizing agent.
Study on Expression and Characterization of HRD3 Gene Related DNA Repair from Eukaryotic Cells
Shin, Su-Hwa ; Park, In-Soon ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 325~330
DOI : 10.5352/JLS.2004.14.2.325
The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (Homologue of RAD3 gene). The over-expressed HRD3 protein was estimated to be a 75 kDa in size which is in good agreement with the estimated by the nucleotide sequence of the cloned gene. Two-dimensional gel electrophoresis showed that a number of other protein spots dramatically disappeared when the HRD3 protein was overexpressed. The overexpressed RAD3 protein showed a toxicity in E. coli host, suggesting that this protein may be involved in the inhibition of protein synthesis and/or degradation of host protein. To determine which part of HRD3 gene contributes to the toxicity in E. coli, various fusion plasmids containing a partial sequence of HRD3 and lac'Z gene were constructed. These results suggest that the C-terminal domain of HRD3 protein may be important for both toxic effect in E. coli and for its role in DNA repair in S. pombe.
Ecological Study on the Seaweed Porphyra pseudolinearis Originated from the East Sea, Korea
Journal of Life Science, volume 14, issue 2, 2004, Pages 331~338
DOI : 10.5352/JLS.2004.14.2.331
Growth and sexual differentiation of the seaweed Porphyra pseudolinearis Ueda have been investigated monthly in the intertidal zone of the East Sea, Korea. Young blades of P. pseudolinearis appeared at the beginning of October. Carp os pores were released at the end of November immediately after carposporangia formation. Then the thalli of P. pseudolinearis were extinguished at the end of March. Young thalli were budded through the stages of conchocelis and conchospore. Thalli showed lanceolate type in shape, cordate type in holdfast, absence of microscopic spinulate process and sexual generation. Ratios of length to width in female thalli ranged from 5.6 to 7.4 at the maturation in December and slightly decreased 3.3 to 4.8 in January and 4.9 to 7.3 in December while the ratios of male thalli ranged from 4.2 to 4.8 in January. On October 12, average five individuals were obsered in a quadrate (30 cm
30 cm), 238
18 individuals for the maturation stage in December and then it was reduced to 150 individuals in February and 15 individuals in March. Average sex ratios for female, male and vegetative thalli were 31.3% 46.9% and 21.9% respectively in early December, the beginning time of sex maturation. The sex ratio of female and male thalli in December 17, changed to 69.4%, 30.6% respectively.
Quantitative Trait Loci for Stem Length in Soybean Using a Microsatellite Markers
Kim, Hyeun-Kyeung ; Kang, Sung-Taeg ; Kong, Hyeun-Jong ; Park, In-Soo ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 339~344
DOI : 10.5352/JLS.2004.14.2.339
Identification of individual quantitative trait loci (QTL) is a prerequisite to application of marker-assisted selection for stern length. Two simple sequence repeat (SSR)-based linkage maps were constructed from recombination inbred line populations between cross of Keunolkong and Shinpaldalkong. Two parents used differed greatly in stem length, which were 30.57 cm and 49.75 cm in Keunolkong and Shinpaldalkong, respectively. Using the constructed maps, regression analysis and interval mapping were performed to identify QTLs conferring stem length. Four QTLs for stem length on linkage groups (LG) F, J, N and O were identified in the Keunolkong
Shinpaldalkong population and they totally explained 37.83% of variation for stem length. In the population, two major QTLs on LG J and O conditioning 14.25% and 10.68% of the phenotypic variation in stem length were determined and two QTLs with minor effect were detected on LG F and N. Identification of QTLs for stem length and mapping individual locus should facilitate to describe genetic mechanisms for stem length in different population. SSR markers tightly linked to QTLs for stem length allow to accelerate the elimination of deleterious genes and selection for desirable recombinants at early stage in crop breeding programs.
Spatial Autocorrelation within a Korean Population of Alnus hirsuta
Park, Joo-Soo ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 345~350
DOI : 10.5352/JLS.2004.14.2.345
The present study was investigated microgeographic variations to spatial autocorrelation in the Korean alder, Alnus hirsuta. Separate counts of each type of join (combination of genotypes at a single locus) for each allele, and for each distance class of separation, were tested for significant deviation from random expectations by calculating the Standard Normal Deviation (SND). Moran's I was significantly different from the expected value in 24 of 120 cases (20.0%). 17 of these values (14.2%) were significantly negative, indicating genetic dissimilarity among pairs of individuals in the ten distance classes. Many Korean populations of alder are small and are distributed by men for firewood. This occasional cutting of seed-bearing stems may bring a high level of gene flow. In addition, stump sprouting ability also may contribute to the fact that the Chengkwang population at Gijang is unusual in lacking spatial genetic structure.
Synthesis of the New Saccharin Derivatives Containing Imidazolidine-2,4,5-trione or 2-Thio-imidazolidine-4, 5-dione Group
Jung, Dai-Il ; Byun, Suk-In ; Kim, Yun-Young ; Kim, Young-Hwan ; Lee, Do-Hun ; Song, Hyun-Ae ; Lee, Yong-Gyun ; Park, Yu-Mi ; Choi, Soon-Kyu ; Han, Jung-Tae ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 351~355
DOI : 10.5352/JLS.2004.14.2.351
Saccharin derivatives were synthesized by means of 4 reaction steps involved the reaction of 1-methylurea (or 1-methylthiourea) and oxalyl chloride. 1-Alkyl(or phenyl)-3-(1,1,3-trioxo-1,3-dihydro-1
-benzo[d]isothiazol-2-ylmethyl)-imidazolidine-2,4,5-trione 5 and 1-alkyl(or phenyl)-2-thioxo- 3-(1,1,3-trioxo-1,3-dihydro-1
-benzo-[d]isothiazol-2-ylmethyl)-imidazolidine-4,5-dione 12 were obtained by means of 4 reaction steps involved the reaction of 1-methyl-urea(or 1-methylthiourea) and oxalyl chloride.
The Effect of Porphyra tenera Extract on Serum Lipid Content in Ovariectomized Rats
Journal of Life Science, volume 14, issue 2, 2004, Pages 356~361
DOI : 10.5352/JLS.2004.14.2.356
The aim of this study is to evaluate the effect of Porphyra tenera (PT) extract on serum lipids content in ovariectomized rats. From day 3 until 42 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups: sham-operated rats (Sham), ovariectomized control rats (OVX-control), ovarjectomized rats supplemented with PT at 50 mg/kg bw/day (OVX-PT50), 200 mg/kg bw/day (OVX-PT200). The PT ethanol extract were orally administrated at 1 m1 per day. Body weight gain, food intake and food efficiency ratio were not significantly different between groups. Although total-cholesterol was increased in OVX-control, supplementation with the PT decreased the level of cholesterol in serum. Especially, it was significantly decreased in OVX-PI50 (p＜0.05). Triglyceride was significantly decreased to almost all of groups supplemented with PT (p＜0.05). The levels of HDL-cholesterol was higher in PT extract group than OVX-control. These results suggest that supplementation with the PT extract positively influenced on lipid concentration in serum. Consequently, we expect that PT extract decrease risk of cardiovascular diseases by postmenopausal.
Construction and Characterization of the Vibrio parahaemolyticus Collagenase Inactivated Mutant
Journal of Life Science, volume 14, issue 2, 2004, Pages 362~367
DOI : 10.5352/JLS.2004.14.2.362
For better understanding of the host infection mechanism of Vibrio, a Vibrio parahaemolyticus collagenase mutant was generated by insertional inactivation of a vppC gene encoding extracellular collagenase. A recombinant DNA containing vppC::nptII was cloned into a suicide plasmid pDMS197, resulted in pVCM03. The recombinant suicide plasmid pVCM03 contained in E. coli
7213 was transferred to a wild-type V. parahaemolyticus 04 through conjugation. The recombinant vppC::nptII DNA in pVCM03 was exchanged with wild-type allele by homologous recombination resulting vppC mutant, V. parahaemolyticus CM. The mutant was selected and screened on TCBS media containing 10% sucrose and kanamycin. The mutation by allele exchange was confirmed with the comparison of the size of DNAs amplified by PCR. V. parahaemolyticus CM showed at least 4-fold less collagen-degrading activity than those of wild-type, and the mutant exhibited less cytotoxicity than that of wild-type in MTT assay.
Expression of Phosphinothricin Acetyltransferase Gene in Transgenic Rice Plants
Lee, Soo-In ; Lee, Sung-Ho ;
Journal of Life Science, volume 14, issue 2, 2004, Pages 368~373
DOI : 10.5352/JLS.2004.14.2.368
We have obtained fertile transgenic rice plants resistant to the broad spectrum herbicide Bast
(active ingredient phosphinothricin, PPT) by PEG-mediated transformation procedure. The plasmid pCaMV35S::Bar was used to deliver the bar gene into embryogenic suspension culture-derived protoplasts of rice (Oryza sativa L.). Transformed plants were regenerated and selected on medium containing 15 mg/l of phosphinothricin. Stable integration and expression of the bar gene in transgenic rice plants was confirmed by Southern and Northern blot analysis. Transgenic
plants were also confirmed by assays for phosphinothricin acetyltransferase (PAT) activity. The bar gene was expressed in the primary transgenic rice plants and in the next generation progeny, in which it showed a 3 : 1 Mendelian inheritance pattern. Transgenic
plants were resistant to the herbicide Bast
when sprayed at rates used in field practice.ice.