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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 14, Issue 6 - Dec 2004
Volume 14, Issue 5 - Oct 2004
Volume 14, Issue 4 - Aug 2004
Volume 14, Issue 3 - Jun 2004
Volume 14, Issue 2 - Apr 2004
Volume 14, Issue 1 - Feb 2004
Selecting the target year
Organ Induction by Combined Dose of bFGF and HGF in Animal Cap Assay of Early Xenopus laevis Embryos.
Journal of Life Science, volume 14, issue 3, 2004, Pages 375~384
DOI : 10.5352/JLS.2004.14.3.375
Fibroblast growth factors (FGFs) are known to induce multiple functions in early development, including mesoderm formation, gastrulation movement and antero-posterior patterning. The induction of mesoderm from Xenopus presumptive ectoderm and the combination effect on inducing organs of bFGF(basic FGF) and HGF (Hepatocyte Growth Factor) were studied. Explants were cultured in the combined solution for 3 days to normal embryo arrive at St. 43. These effects on combined dose were examined by histological experiment and by immunohistochemical method. The concentrations of growth factors were tested in 0, 0.5, 1, 10 and also tested in 50 ng/ml of bFGF, and 0, 1, 10, 50 and 100ng/ml of HGF respectively. The synergistic effects were seen in the combined-dose of bFGF and HGF rather than in single dose. Various organs were differentiated and highest inducing effects were seen at the combined concentration of 1 ng/ml of bFGF and 10ng/ml of HGF, and at the concentration 10ng/ml of bFGF and 1 ng/ml of HGF. The bFGF induces various organs from cultured animal cap explants and the effects are time and dose-dependent. HGF is also a potent mitogen for renal tubular cells and for mature hepatocytes in primary culture. Eyes were developed in high percentage at the combined concentration of 1 and 10ng/ml of bFGF, and 1 and 10 ng/ml of HGF. From the induced eye and normal embryonic eye, RPE65 was commonly detected by monoclonal antibodies 40All and 25F5 and the localization of RPE65 was seen by AP reaction.
Effects of Chitosan on Fracture Healing in Fractured Rat Model.
Journal of Life Science, volume 14, issue 3, 2004, Pages 385~390
DOI : 10.5352/JLS.2004.14.3.385
Chitosan is abundant polysaccharide polymer in nature Chitosan has been the subject of much research regarding its potential as a nutritional adjunct and pharmaceutical ingredient. In the present study, we examined fracture-healing process with chitosan administration and low calcium diet in rats. Left femur was fractured and fixed with intramedullary pin. The rats were fed normal diet or low calcium diet and administered chitosan with the doses of 0, 50, 100 and 150ng/ml orally 5 times a week for 10 weeks. Soft X-ray and mechanical testing of all fractured femora was taken. Radiographical finding showed that the callus formation and process of fracture healing was present in all the fractured femora. Mechanical testing indicated that the maximum load and stiffness of femur in rat fed low calcium diet was lower than those of that in rat fed normal diet. No difference in maximum load and stiffness of fractured femora in chitosan treated rat were observed as compared to vehicle treated rat. Chitosan or/and low calcium diet did not affect the ratio of fractured/unfractured femur about maximum load and stiffness. The results suggest that chitosan dose not affect the bone mechanical strength and the process of fracture healing. Low calcium diet does decrease the bone mechanical strength.
Constitutive Expression of Bacillus stearothermophilus CGTase in Bacillus subtilis.
Journal of Life Science, volume 14, issue 3, 2004, Pages 391~395
DOI : 10.5352/JLS.2004.14.3.391
To overproduce the cyclodextrin glucanotransferase(CGTase) of Bacillus stearothermophilus NO2 in B. subtilis, the pJH-CGTl plasmid (8.14 kb) was constructed, in which the ORF of CGTase gene could be transcribed by strong constitutive promoter, P
JH/. To overproduce CGTase from a recombinant B. subtilis, the effect of media on the cell growth and expression level of CGTase were investigated with various media (LB, 2
LB, 5% molasses+2% CSL, CS, LBG) in the flask culture. Among them, [5% molasses+2% CSL] medium revealed the maximum expression level of CGTase with 1.8 unit/
at 9 hr culture. In the batch culture on [10% molasses+5% corn steep liquor] medium the expression level of CGTase, the secretion efficiency, and plasmid stability were about 4.2 unit/
, 90% and 90%, respectively, at 30 hr culture. The cell growth and expression level in the fermenter culture with the industrial molasses medium were increased by 2-folds over the flask culture.
Changes of Root System in Rice (Oryza sativa L.) Plant Under Salt- and Drought- Stressed Agar Medium Conditions.
Journal of Life Science, volume 14, issue 3, 2004, Pages 396~399
DOI : 10.5352/JLS.2004.14.3.396
This study was investigated the changes of root length, tissue structure of root tip, and dry matter production of a Dongjinbyeo (DJ) cultivar subjected to 0.4 % agar medium with various concentration of NaCl (salt stress)- and polyethylene glycol 6000 (PEG, drought stress). Root length and dry weight of DJ plant were declined along the high concentration of PEG and NaCl in rice plants. To elucidate the changes of tissue structure in root tip to PEG- and NaCl-treatments, we examined the microscopic observation of root tip in NaCl- and PEG-treated rice plants using Toluidine blue O. By Toluidine blue O staining, methyl-lignin accumulation was found in the epidermis and outer cortex of the elongation zone at an early stage of PEG treatment, whereas was found only the outer cortex of the elongation zone of NaCl-treated root tip. The epidermis of NaCl-treated root tip became soften instead of methyl-lignin accumulation. TR ratio was increased along the high concentration in PEG- and NaCl-treated rice plant as a result of inhibited root elongation under PEG- and NaCl-treatment. From these morphological changes in root stimulated by drought and salt stress, we suggest that agar medium is useful to identify tolerant variety in germination stage under stressful environments.
Morphological Analysis on the Kalopanax pictus (Araliaceae) of Korean Populations
Jung, Sang-Duk ; Hong, Jung-Hee ; Bang, Kyung-Hwan ; Huh, Man-Kyu ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 400~405
DOI : 10.5352/JLS.2004.14.3.400
Morphological characteristics of Kalopanox pictus Nakai were studied to examine population differentiation of this species. Based on a phenogram of using 23 morphological characteristics, differentiation of regions were distinct. Collections of 138 specimens from nine populations served as operational taxonomic unit (OTU's) were examined for phenotypic similarity and morphological variation using clustering (Ward's minimum variance method) and principal component analysis (PCA). The first three principal components were responsible for 77.0% of the total variance. Principal component 1 explained 52% of the total variance and was contributed to by the number of palmately parted, the number of pinnately lobed, and width between two lateral lobe apex.
Increased DNA Damage Induced by Glycation Propagator
Journal of Life Science, volume 14, issue 3, 2004, Pages 406~410
DOI : 10.5352/JLS.2004.14.3.406
Glyoxal or methylglyoxal was incubated with catalase in 0.24 M sodium phosphate buffer (pH 7.4) at 37
. Dicarbonyls modify and inactivate catalase. Plasmid DNA that is directly incubated with glycation propagators, glyoxal and methylglyoxal, showed different DNA mobility shift compared to nomal plasmid DNA. When plasmid DNA is added in Fenton reaction with glycated catalase, plasmid DNA was significantly strand broken and 8-hydroxydeoxyguanosine production was time dependently increased. These results suggest that glycation of antioxidant is synergistic effect to oxidative stress.
Studies on the Antioxidative and Antimicrobial Effects of Chondria crassicaulis
Journal of Life Science, volume 14, issue 3, 2004, Pages 411~416
DOI : 10.5352/JLS.2004.14.3.411
In this study, we investigated the biological activity of antioxidant and antimicrobiological effect of Chondria crassicaulis (CC), which, using methanol, dichlolometane and ethanol, were extracted and fractionated into four different types: hexane(CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. The reducing activity on the 1,I-diphenyl-2-picrylhydrazyl (DPPH) radical and
radical scavenging potential, in search for antioxidation effects of CC partition layer, were sequentially screened. Among the four fractions, CCMM had the highest antioxidative activity. The antimicrobial activity was increased in proportion to its concentration by the paper disc method. Among the various solvent layers, the CCMB, CCMH and CCMM showed relatively strong antimicrobial activities in the order.
Lectin Histochemistry of the Glycoconjugates in the Esophageal Mucous Cells of Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis
Journal of Life Science, volume 14, issue 3, 2004, Pages 417~424
DOI : 10.5352/JLS.2004.14.3.417
This study attempts to investigate lectin binding patterns of the glycoconjugates of the esophageal mucous cells in four teleostean speceis, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis. To investigate glycoconjugates of esophageal mucous cells, nine biotinylated lectins (DBA, SBA, PNA, BSL-l, RCA-l, sWGA, UEA-l, LCA and ConA) were applied with ABC method. Esophageal mucous cells of Sebastes schlegeli and Halichoeres poecilopterus were mixed with large, medium sized and small mucous cells. But these cells of the other species only were mixed with medium sized and small mucous cells. The lectin binding pattern of esophageal mucous cells depends on the species; Sebastes schlegeli was stained with DBA, SBA, BSL-l, RCA-l and sWGA, Halichoeres poecilopterus with DBA, SBA, PNA and sWGA, Bryzoichthys lysimus with SBA and sWGA, Takifugu pardalis with all lectins except DBA, LCA and Con A, respectively. All the mucous cells of Sebastes schlegeli were stained with DBA, SBA and sWGA, while small mucous cells with BSL-l besides these lectins. In Halichoeres poecilopterus,l the large mucous cells reacted with PNA, medium sized mucous cells with DBA, SBA and sWGA, and small mucous cells with DBA and SBA, respectively. Medium sized mucous cells of Bryzoichthys lysimus were stained with sWGA, and small mucous cells with SBA and sWGA. In Takifugu pardalis, all mucous cells reacted with SBA, PNA and RCA-l, but medium sized mucous cells with sWGA and UEA-l besides these lectins. Especially DBA and SBA lectins showed a strong binding to all mucous cells of Sebastes schlegeli. In Halichoeres poecilopterus, PNA binding were notable in large mucous cells, and SBA binding in medium sized and small cells, respectively. However, SBA, PNA, sWGA and UEA-l lectins of Takifugu pardalis showed a strong binding to medium sized mucous cells, but RCA-l binding which small mucous cells were notable.
Molecular Authentication and Genetic Polymorphism of Korean Ginseng (Panax ginseng C. A. Meyer) by Inter-Simple Sequence Repeats (ISSRs) Markers
Bang, Kyong-Hwan ; Lee, Sung-Woo ; Hyun, Dong-Yun ; Cho, Joon-Hyeong ; Cha, Seon-Woo ; Seong, Nak-Sul ; Huh, Man-Kyu ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 425~428
DOI : 10.5352/JLS.2004.14.3.425
Molecular authentication and genetic polymorphism of Korean ginseng cultivars and accessions were investigated using ISSR (inter-simple sequence repeat amplification) markers. Five primers among 56 produced clear and reproducible DNA fragments among seven cultivars and accessions. A total of 43 bands ranging from 250 bp to 1,700 bp from five primers were scored. Average number of bands per primer was 8.6 and only nine bands were polymorphic across the six Panax ginseng from Korea. Especially Chunpoong cultivar exhibited the highest level of polymorphism, whereas other accessions did not showed almost any polymorphism. Consequently, these ISSR markers will be available to differentiate Chunpoong cultivar from other major Korean ginseng cultivars and accessions, such as Yunpoong, Hwangsukjong and Jakyungjong, at the DNA level.
Expression and Secretion of Zymomonas mobilis Levansucrase in Saccharomyces cerevisiae.
Journal of Life Science, volume 14, issue 3, 2004, Pages 429~434
DOI : 10.5352/JLS.2004.14.3.429
Levansucrase gene (levU) from Zymomonas mobilis was subcloned downstream of GALl promoter in pYES 2.0 and pYInu-AT [GALl0 promoter＋exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-levU and pYInu-levU, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Saccharomyces cerevisiae SEY2102, and then transformants showing high activity of levansucrase were selected. When each yeast transformants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 7.17 U/㎖ with the strain harboring pYES-levU and 6.61 U/㎖ with the strain harboring pYInu-levU. It was found that about 50% of levansucrase were detected in the medium and periplasmic space, and exoinulinase signal sequence didn't enhance the secretion efficiency. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.
Overexpression of the get Gene Encoding 4-α-Glucanotransferase of a Hyperthermophilic Archaeon, Thermococcus litoralis
Jeon, Beong-Sam ; Park, Jeong-Won ; Shin, Gab-Gyun ; Kim, Beom-Kyu ; Kim, Hee-Kyu ; Song, Jae-Young ; Cho, Young-Su ; Cha, Jae-Young ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 435~440
DOI : 10.5352/JLS.2004.14.3.435
The gene encoding a extremely thermostable 4-
-glucanotransferase from a hyperthermophilic archaeon, Thermococcus litoralis, was cloned, sequenced and expressed in Escherichia coli. The amino acid sequence of the enzyme was distantly related to other functionally-related ones, such as D-enzymes. The enzyme is of industrial interest because of a novel activity of producing cycloamylose and is also important for fundamental studies of protein, sugar-metabolizing enzymes. In this paper, the overexpression of 4-
-glucanotransferase in E. coli was carried out expression vector system with lac and T7 promoters. The enzyme was successfully overexpressed, and purified by the heat treatment of a cell-free extract, successive Butyl-Toyopearl and Mono Q chromatographies. The purified recombinant enzyme showed the same specific activity and the same mobility in SDS-PAGE as natural enzyme.
The Novel Assay Method for Thrombin by Weighing Fibrin Clot
Park, Inshik ; Kim, Gi-Nahm ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 441~444
DOI : 10.5352/JLS.2004.14.3.441
This study was performed to establish a simple and rapid method for measuring thrombin activity based on weight of fibrin clot formed. The new method was based on the weight measurement of fibrin clot after enzymatic reaction of thrombin with fibrinogen. The fibrin formation depended upon the activities of thrombin used, temperature, incubation time, and centrifugation time. The fibrin formation was increased proportionally up to 1.0 unit/ml of thrombin activity, 4.0mg/ml of fibrinogen concentration, and 5 min of incubation time at 37
. The fibrin clot formed was stable by centrifugation at 3,000
g for 5min. This simple assay based on weight of fibrin after centrifugation would be useful for identifying natural food anticoagulants by inhibiting thrombin.
Regulation of Bcl-2 Family and Cyclooxygenases by Furanoterpenoids Isolated from a Marine Sponge Swcotragus nt. in Human Lung Cancer A549 Cells.
Journal of Life Science, volume 14, issue 3, 2004, Pages 445~452
DOI : 10.5352/JLS.2004.14.3.445
We investigated the cytotoxic effects of seven furanoterpenoids 〔sarcotin A, epi-sarcotin A, ircinin-1, epi-sarcotrine B, sarcotin I, (8E, l3Z, 20Z)-strobilinin/(7E,l3Z, 20Z)-felixinin and (7E,12E,18R,20Z)-variabilin〕 isolated from the sponge Sarcotragus sp. (the order Dictyoceratida) on the growth of A549 human lung carcinoma cells. MTT data revealed that sarcotin A and (7E,12E,18R,20Z)-variabilin exhibited higher potencies on the anti-proliferative activities than the other compounds in A549 cells. The growth inhibition by treatment with compounds (especially epi-sarcotin A, ircinin-1 and epi-sarcotrine B) were associated with the induction of apoptotic cell death through the concentration-dependent increase of Bax/Bcl-2 ratio in a p53-dependent or independent pathway Additionally, epi-sarcotin A and ircinin-1 strongly inhibited the levels of cyclooxygenase (COX)-2 expression without alteration of COX-1. Taken together, the results suggest that the furanoterpenoids from the marine sponge have strong potentials as candidates for anti-cancer drugs.
Identification of Quantitative Trait Loci (QTLs) Associated with Oil and Protein Contents in Soybean (Glycine max L.)
Kim, Hyeun-Kyeung ; Kang, Sung-Taeg ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 453~458
DOI : 10.5352/JLS.2004.14.3.453
Soybean oil and protein contents are very important as a nutritional component of food. The seed composition as oil and protein are polygenic traits. In this study, the Keunolkong
Iksan10 populations were evaluated with SSR markers to identify QTLs related to oil and protein contents. Three related independent QTLs near the marker satt100 on LG C2, satt546 on LG D1b＋W and satt418 on LG L were identified oil contents. The three independent QTLs near the marker satt556 on LG B2, satt414 on LG J and satt238 on LC L were identified of protein contents. In the results of this study, common QTLs on LG L was associated with seed oil and protein contents. In the result of this study, it is believed that the seed composition material as oil and protein contents were mainly controlled by environmental stresses and they are seed size on genotypes.
Plant Population Structure of Mt Jeolyoung in Young-do of Busan
Huh, Hong-Wook ; Park, Joo-Soo ; Huh, Man-Kyu ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 459~466
DOI : 10.5352/JLS.2004.14.3.459
The purpose of the precent investigation was to investigate diversity patterns in a fragmented Young-do forested landscape. It was conducted on sloping 18 plots (twelve 50
50 m plots and six 10
100 m plots). Total 1562 individuals were identified and measured in the 50
50 m plots. These were a total of 68 species of 23 families present in the three sites. Least significant differences (LSD) by post hoc analysis revealed that fragments site A had significantly greater densities than site B. Shannon-Wiener functions differed significantly among forests (F=4.12, p＜0.05), with site A forest having significantly higher value (3.401) than the others (2.590 for site B and 2.648 for site C). Pinus thunbergii and Quercus aliena were dominant on most sites. site. The Pinus densiflora forest of sites A and C is distributed better in the NE exposures compared with the opposite ones. Ficus erecta and Pinus densiflora largely occupied the southern exposures. The spatial distribution in southern Young-do's littoral forest was very heterogeneous, reflected by most species having low densities and patchy distributions.
Isolation of Antimicrobial Substance by Produced Bacillus sp. SD-10 with Antagonistic Activity Towards Mushroom Pathogens
Journal of Life Science, volume 14, issue 3, 2004, Pages 467~471
DOI : 10.5352/JLS.2004.14.3.467
Bacillus sp. SD-10 was investigated to develope biological pesticides for control of mushroom diseases. Bacillus sp. SD-10 showed high antifungal activity when cultured at 35∼4
. The culture filtrate of the bacterium inhibited the growth of mycelium of T. virens which is a kind of mushroom pathogene. On the test of inhibition of spore germination of T. virens, more than 5% of the culture filtrate in the media inhibited completely the germination of the spores. An antimicrobial substance, UPX-1 was purified from the culture filtrate of the Bacillus. From the
-NMR spectrum analysis, the substance was indentifed as disaccharide composed to six carbon sugars. UPX-1 has not only strong antifungal activity against T. virens but also antibacterial activity against Pseudomonas tolaassi.
Improved Astaxanthin Production of Xanthophyllomyces dendrorhous with Carotenogenesis Stimulating Factors
Xu, Bao-Jun ; Sung, Chang-Keun ; Li, Chang-Tian ; Mo, Eun-Kyoung ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 472~477
DOI : 10.5352/JLS.2004.14.3.472
To improve biomass and astaxanthin production by wild-type Xanthophyllomyces dendrorhous simultaneously in shake flask culture, physical factors, nutritional factors and carotenogenesis stimulating factors affecting astaxanthin production were studied on base of HPLC quantitative analysis. The results suggested that carotenogenesis precursor composition acetic acid, mevalonic acid, tomato extract, and carrot extract could increase the productivity of astaxanthin markedly based on the optimized temperature, initial pH value, carbon and nitrogen sources conditions.
The effect of L-Ascorbic Acid on the Oxidative Reaction of Lysine in Collagen.
Journal of Life Science, volume 14, issue 3, 2004, Pages 478~483
DOI : 10.5352/JLS.2004.14.3.478
In a model reaction using lysyl oxidase purified partilally from bovine aorta, effect of L-ascorbic acid AsA on the oxidative reaction of lysine in collagen was investigated. Addition of Ash to the reaction mixture under aerobic conditions resulted in the decrease of enzymatic activity. In order to examine the specificity of AsA in the oxidative reaction of lysine, other reductants including A derivatives instead of AsA were added to the reaction mixture. Thiol such as glutathione had no effect on the activities of lysyl oxidase. on the other hand, it was observed that erythorbic acid, which was a stereoisomer of AsA, had the same inhibitory effect on this oxidative reaction as AsA. Moreover, by the addition of 3,4-dihydroxybenzoate, which was structural analog of AsA, the activities decreased in a similar manner to that of AsA. These results indicate that the regulatory effect of AsA on lysyl oxidase is attributed to characteristics of the structure. From the determination of Ash remained in the reaction mixture, it is shown that AsA concentration remarkably decreased by lysyl oxidase of the reaction mixture. It is hypothesized that endiol groups reduces the enzyme-bound
required for further progress of the reaction, and suggests that AsA regulates specifically the reduction of
required to oxidize lysyl oxidase. This findings support that AsA has an important regulatory role on the oxidative reaction of lysine and on changes of collagen cross-links with aging.
Strength Evaluation of Sin91e-Radius Total Knee Replacement (TKR)
Wan, Jin-Young ; Sub, Kwak-Yi ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 484~489
DOI : 10.5352/JLS.2004.14.3.484
Artificial joint replacement is one of the major surgical advances of the 21th century. The primary purpose of a TKA (Total Knee Arthroplasty) is to restore normal knee Auction. Therefore, ideally, a TKA should: (a) maintain the natural leverage of the knee joint muscles to ensure generating adequate knee muscle moments to accomplish daily tasks such as rising from a chair or climbing stairs;(b) allow the same range of motion as an complete knee; and (c) provide adequate knee joint stability. Four individuals (2 peoples after surgery one year and 2 peoples after surgery three years) participated in this study. All they were prescreened for health and functional status by the same surgeon who performed the operations. Two days of accommodation practice occurred prior to the actual strength testing. The isometric strength (KIN-COM III) of the quadriceps and hamstring were measured at 60
of knee flexion, respectively. During isokinetic concentric testing, the range of motion was between 10
of knee flexion (stand-to-sit) and extension (sit-to-stand). for a given test, the trial exhibiting maximum torque was analyzed. A 16-channel MYOPACTM EMG system (Run Technologies, Inc.) was used to collect the differential input surface electromyographic (EMG) signals of the vastus medialis (VM), vastus lateralis(VL), rectus femoris (RF) during sit-to-stand and stand-to-sit tests. Disposable electrodes (Blue SensorTM, Medicotest, Inc.) were used to collect the EMG signals. The results were as follows; 1. Less maximum concentric (16% and 21% less for 1 yew man and 3 years mm, respectively) and isometric (12% and 29%, respectively) quadriceps torque for both participants. 2.14% less maximum hamstrings concentric torque for 1 year man but 16% greater torque for 3 years mm. However, 1 year man had similar hamstring isometric peak torque for both knees. 3. Less quadriceps co-contraction by 1 year man except for the VM at 10
range of knee flexion.
Effect of Amino Acid Solution for Cell Growth and MPS Activity of Mineral Phosphate Microorganisms.
Journal of Life Science, volume 14, issue 3, 2004, Pages 490~495
DOI : 10.5352/JLS.2004.14.3.490
Phosphate-solubilizing activities of the two strains (Burkholderia sp. DA23 and Klebsiella sp. DA7l-1) against tri-calcium phosphate and hydroxyapatite were quantitatively determined. Two strains were found to solubilize two types of insoluble phosphate different amounts of amino acid solutions in liquid culture. MPS ability of the strains was increased with concentration of amino acid addition. The optimal solubilization condition of insoluble phosphate in sucrose minimal medium were 0.1% amino acid solution, respectively. The efficiency of amino acid addition was no difference between the two types of insoluble phosphate, tri-calcium phosphate and hydroxyapatite.
A Study on Physico-chemical Property and Composition of Velvet Antler and Byproducts in Elk.
Journal of Life Science, volume 14, issue 3, 2004, Pages 496~500
DOI : 10.5352/JLS.2004.14.3.496
This study has been performed for physico-chemical property and composition of sinew, testes, tail, blood and velvet antler in Elk. Amino acid contents in the sinew taurine, alanine, histidine, and lysine were high contained, histidine, glutamic acid, taurine, and lysine were high contained in testes, glutamic acid, lysine, alanine, glycine, and phenylalanine were high contained in tail, histidine, glycine, and lysine were high contained in blood, glutamic acid, lysine, taurine, alanine, and glycine were high contained in velvet antler. And, based on the amount of mineral,
were 444.8, 166.6 and 242.9mg per 100g in sinew, respectively.
were 294.4, 330.5, and 514.3 mg per 100g in testes, respectively,
were 1420.6, 118.4, and 1105.2mg per 100 mg in tail, respectively. Fe3+,-K+, and P+ were 344.1, 1023.6 and 157.2 mg per 100 mg in blood, respectively and
were 888.4, l1533.1 and 14722.0 in velvet antler, respectively. Finaly, difference were found in comparison of composition of free mwtal ion (N
) bwteen blood, tail, tests, sinew and velvet antler, and composition of free metal in blood higher than those othersd higher than those others
Isolation and Characterization of Chitinoloytic Strain, Bacillus atrophaeus CJ-3.
Journal of Life Science, volume 14, issue 3, 2004, Pages 501~508
DOI : 10.5352/JLS.2004.14.3.501
A bacterial strain CJ-3 which produced chitinase was isolated from Korean traditional soy sauce. Using 16S rDNA analysis, the strain CJ-3 was identified as Bacillus atrophaeus. The approximate molecular weight of the putative chitinase enzyme was 31.0 kDa and the enzyme activity was remarkably induced by addition of colloidal chitin (0.5, 1.0, 2.0%). The antioxidant activity was increased 53% by the browning reaction products of B. atrophneus CJ-3. Escherichia. coli lipopolysaccharides (LPS)-induced production of nitric oxide(NO) was reduced up to 45% by the browning reaction product in RAW264.7 macrophage. Inhibition of cell viability in the presence of LPS was recovered to normal level by the browning reaction product. These results suggest that browning reaction of B. atrophaeus CJ-3 plays an important role for activation of immune system. B. atrophaeus CJ-3 exhibited optimum temperature and pH of 37
and pH 7.0∼8.0, respectively. The major intracelluar free amino acid was determined to be glutamate.
Inhibitory Activities of Edible and Medicinal Herbs Against Human Thrombin
Journal of Life Science, volume 14, issue 3, 2004, Pages 509~513
DOI : 10.5352/JLS.2004.14.3.509
Inhibitory activities of 58 methanol extracts, which were prepared from different parts of 55 kinds of edible and medicinal plants, against human thrombin were evaluated. Analysis of the anti-coagulation activity determined by thrombin time enabled us to select 8 herb extracts as possible sources of anti-thrombosis agent. Further analysis of anti-thrombosis activity determined by activated partial thromboplastin time and fibrinolytic activity, and heat stability and inhibition against other proteolytic digestive enzymes provided new information that Pimpinella brachycarpa, Lysimachia clethroides, and Salix gracilistyla could be used as a potential anti-thrombosis agent. Our results suggest that edible and medicinal plants could be the potential source of thrombin inhibitor.
Antioxidative Effects of Mushroom Extract and Fermented Milk Containing Its Extract on in vivo and in vitro Lipid Peroxidation
Journal of Life Science, volume 14, issue 3, 2004, Pages 514~520
DOI : 10.5352/JLS.2004.14.3.514
The antioxidative effects of fermented milk, mushroom extract and fermented milk containing its extract (Lentinus edodes, Ganoderna lucidum, and Pleurotus ostreatus) on the lipid peroxidation in the tissues of female Sprague-Dawley rats and on the DPPH (
-picrylhydrazyl) radical donating ability were studied. The total concentrations of polyphenolic compound in Lentinus edodes, Ganoderma lucidum and Pleurotus ostreatus were 0.34, 0.20 and 0.34%, respectively. The DPPH donating abilities of mushroom extract, fermented milk, fermented milk containing its extract and BHT (butylated hydorxytoluene) as standard were 33.9, 34.9, 51.9 and 95.6%, respectively. Experimental diet groups were divided into five groups: the normal diet (ND), the cholesterol diet (CD), and cholesterol + fermented milk diet (CDFM), cholesterol + mushroom extract diet (CDME) and cholesterol + fermented milk containing mushroom extract diet (CDFMME). The concentrations of lipid peroxide in liver and its microsome were significantly lower in both CDFM and CDFMME groups than in the other groups. The kidney concentration of lipid peroxide was significantly higher in the CD group than in the ND group, but this rise were significantly decreased in the CDFM and CDFMME groups. Meanwhile, the concentrations of heart and spleen and their fractions were not significantly different among dietary groups. This study was suggested that the fermented milk diet containing mushroom extract effectively reduced the lipid peroxidation in liver and kidney of cholesterol-fed female rats.
Genetic Diversity Analysis of the Cheju Horse Using Random Amplified Polymorphic DNAs
Cho, Byung-Wook ; Lee, Kil-Wang ;
Journal of Life Science, volume 14, issue 3, 2004, Pages 521~524
DOI : 10.5352/JLS.2004.14.3.521
This experiment was carried out to analyze genetic characteristics and to develop the breed specific DNA marker for Cheju-native horse. If this marker contains high repetitive sequences, it is possible to convert a RAPD marker of interest into a single-locus PCR marker called a sequence characterized amplified region(SCAR). Twenty six Cheju-native horse and Fifty thoroughbred genomic DNA were pooled and PCR. were accomplished using 800 random primers. Comparing the pooled DNA from Cheju-native horse and thoroughbred, we found 9 primers which identified markers present in the pooled DNA from breed but absent in the other breed. Among 9 random primers, 6 primers were thoroughbred specific and 3 primers were Cheju-native horse specific. Testing individual horse revealed that 5 marker showed the similar band pattern between Cheju-native horse and Thoroughbred. However, 4 marker were wholly absent in breed while present in the other breed. UBC
, and UBC
was detected only Thoroughbred and UBC
was detected Cheju-native horse, respectively. After determining of the cloned breed-specific fragment sequence, we designed the SCAR-primers and carried out PCR. Compared to random primer, RAPD-SCAR primer didn't show significantly higher specific band. However, RAPD analysis is useful for genetic characterization of Cheju-native horse.
Physico-Chemical Properties and Composition of Venison in Deer Breeds.
Journal of Life Science, volume 14, issue 3, 2004, Pages 525~530
DOI : 10.5352/JLS.2004.14.3.525
This study has been performed to examine physico-chemical properties and composition of venison in Deer breeds. According the results, moisture content in the venison ranged between 74% and 75%, and there were no significant differences among the breeds, or among the cutting parts. Fat content in the meats was estimated as less than 1% except in loin, protein contents was estimated as more than 22%, and ash content was estimated as more than 1.5%. For the contents of inorganic material in the loin, Fe
content was higher in Elk than in other breeds, the contents of
were higher in Red deer than in others, and the contents of
were higher in Sika deer than in others. For the contents of inorganic material in the leg, the contents of
were higher in Elk than in other breeds, the contents of
were higher in Red deer than in others, and the contents of
were higher in Sika deer than in others. Amino acid contents in the venison alanine, proline, glycine, lycine and histidine were highly contained. and the contents of asparagine, cysteine, isoleucine and threonine were low. For the differences of amino acid contents among the breeds, the level was the higher in Sika deer than those of others. For the fatty acid in loin, the contents of myristic acid, oleic acid and linolenic acid in Sika deer were higher than those of others, the contents of myristic acid, palmitoleic acid, oleic acid and arachidonic acid in Red deer were higher than those of others and in Elk, the contents of palmitic acid and stearic acid were higher than those of others. For the contents of unsaturated fatty acid and polyunsaturated fatty acids, the level was higher in Red deer than those of others. For the fatty acid in leg, the contents of oleic acid, linolenic acid, linolenic acid and arachidonine acid in Sika deer were higher than those of others, the contents of myristic acid, palmitic acid, palmitoleic acid and oleic acid in Red deer were higher than those of others and in Elk, the contents of stearic acid and linolenic acid were higher than those of others. For the contents of saturated fatty acid, the level was lowest in Sika deer. For the contents of unsaturated fatty acid and polyunsaturated fatty acids were respectively highest in Sika deer. Comparing the contents inorganic materials between loin and leg, the concentrations in leg were generally higher than that of loin.n.