Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Life Science
Journal Basic Information
Journal DOI :
Korean Society of Life Science
Editor in Chief :
Volume & Issues
Volume 15, Issue 6 - Dec 2005
Volume 15, Issue 5 - Oct 2005
Volume 15, Issue 4 - Aug 2005
Volume 15, Issue 3 - Jun 2005
Volume 15, Issue 2 - Apr 2005
Volume 15, Issue 1 - Feb 2005
Selecting the target year
Molecular Typing of Legionella pneumophila Isolated in Busan, Using PFGE
Park Eun-Hee ; Kim Mi-Hee ; Kim Joung-A ; Han Nan-Sook ; Lee Ju Hyeoun ; Min Sang Gi ; Park Yon Koung ; Jin Seong Hyun ; Jeong Gu Young ; Bin Jae Hun ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 161~168
DOI : 10.5352/JLS.2005.15.2.161
In this study, we did the molecular typing of 39 environmental Legionella pneumophila serogroup 1 isolates collected from 2001-2003 in Busan using the pulsed-filed gel electrophoresis (PFGE). PFGE of SfiI fragments were divided into 10 pulsotypes
, corresponding to $<65\%$ similarity and a subtype within each pulsotype was characterized by $>84\%$ similarity. The major cluster was pulsotype E
, which included 18 isolates and was divided into 4 subtypes
. PFGE of NotI fragments were divided into 8 pulsotypes
, corresponding to $<60\%$ similarity and a subtype within each pulsotype was characterized by
similarity. The major cluster was pulsotype f
, which included 15 isolates. The ATCC type strain L. pneumophila serogroup 1 was identified as a different molecular pulsotype compare to the Busan isolates. It is possible that L. pneumophila serogroup 1 isolated in Busan with specific DNA pattern is comparable with those isolation in other cities in Korea.
Effect of Resveratrol on the Induction of Cdk Inhibitor p21 and Pro-apoptotic Bax Expression by amyloid-β in Astroglioma C6 Cells
Kim Young Ae ; Lim Sun-Young ; Ko Woo Shin ; Choi Byung Tae ; Lee Yong Tae ; Rhee Sook-Hee ; Park Kun-Young ; Lee Won-Ho ; Choi Yung Hyun ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 169~175
DOI : 10.5352/JLS.2005.15.2.169
Resveratrol (3,4',5-trihydroxy-trans-stilbene), a phytoalexin found in grape skins, peanuts, and red wine, has been reported to have a wide range of biological and pharmacological properties.
deposition and senile plaque-associated astrocytes are common neuropathological features of Alzheimer's disease. In this study, we have explored the effects of resveratrol on
cytotoxicity in vitro and modulation of cell growth-regulatory gene products in astroglioma C6 cells to elucidate its possible mechanism for anti-cytotoxicity. Exposure of C6 cells to
resulted in dose-dependent growth inhibition and morphological changes of C6 cells, which were recovered by pre-treatment with resveratrol. The anti-proliferative effect of
was associated with the induction of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21 (WAF1/CIP1) expression assessed by RT-PCR and Western blot analysis in time-dependent manner in C6 cells. In addition, the pro-apoptotic Bax expression was also up-regulated in
C6 cells without alteration of anti-apoptotic Bcl-2 and
expression. However, pre-treatment of resveratrol significantly inhibited
p53, p21 and Bax levels, suggesting that the modulation of p53, p21 and Bax levels could be one of the possible pathways by which resveratrol functions as anti-cytotoxic agent. Our results demonstrate that resveratrol may enhance the protection against
cytotoxicity by promoting the survival of glial cells.
Tissue Specific Expression of Wound-Inducible RCaM-2 Promoter in Transgenic Tobacco Plants
Choi Young Ju ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 176~181
DOI : 10.5352/JLS.2005.15.2.176
To study calmodulin (CaM) gene expression and its regulation, rice CaM promoter (RCaM-2) was isolated and fused to
(GUS), reporter gene. X-Glue staining patterns revealed that GUS localization is high in meristemic tissues such as the stem apex, stolen tip, and vascular regions. GUS staining in the transverse sections of stem and petiole was restricted to the inside of the vascular system, and cortex and epidermis located outside of the vascular system usually did not show GUS staining even a plant that expressed strong activity. GUS activity was found to be tissue specific expressed and exhibited a dramatic transient increase in response to wounding. These results suggest that the 5'-flanking region of RCaM gene regulates wound-inducible expression.
Mutations in the PPE Genes that Confer Resistance to a Nitroimidazopyran Drug on Mycobacterium bovis Strains
Bae Young-Min ; Daniels Lacy ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 182~185
DOI : 10.5352/JLS.2005.15.2.182
We used the IS1096 transposon to construct Mycobacterium bovis BCG mutants resistant to an antituberculosis drug PA-824 and isolated several different mutants. We identified the locations of the insertions and found that the insertions were at various sites including the genes for the PPE proteins. HPLC analyses of the extracts of these five PPE mutant cells showed that three mutants produced only F0, and intermediate for the synthetic pathway of coenzyme
, and the remaining two neither F0 nor
. These data suggest that the products of these PPE genes are somehow involved in the biosynthesis of the coenzyme
Up-regulation of Galectin-3 in HIV-1 tat-transfected Cells
Yu Hak Sun ; Kim KoanHoi ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 186~191
DOI : 10.5352/JLS.2005.15.2.186
Previous studies have demonstrated that expression of galectin-3, a member of family of beta-galactoside-binding animal lectin, is associated with pathological conditions including cancer, atherosclerosis, and viral infection. An increase of this lectin has been observed after infection by Kirsten murine sarcoma, human T lymphotropic virus-l (HTLV-l), and human immunodeficiency virus-l (HIV-l). Viral transactivation protein Tax of HTLV-l mediates the increase in the lectin. In case of HIV-1, there are evidences that Tat would be related with increase in galectin-3. We investigated whether Tat directly induced galectin-3 expression in cells. We found that HIV-l tat gene activated galectin-3 promoter in RAW264.7 cells. To demonstrate direct induction of galectin-3 by HIV-l tat, we transfected the tat into a rabbit smooth muscle cell line (Rb1) and obtained RblTatCl-2, a clone of cell stably transfected with tat gene. The Rb1TatCl-2 cells exhibited activation of LTR promoter and up-regulation of galectin-3 transcript as well as protein. Our results indicate that HIV-l tat alone is sufficient to induce the expression of galectin-3. The Rb1TatCl-2 cells could be valuable for study of the effect of HIV-1 tat on expression of cellular genes.
Isolation and Characterization of hrp2+ Gene Related to SNF2 Family In Yeast
Choi In Soon ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 192~196
DOI : 10.5352/JLS.2005.15.2.192
The SNF2/SW12 family comprises proteins from a variety of species with in vivo functions, such as transcriptional regulation, maintenance of chromosome stability during mitosis, and various types of DNA repair. This study was shown the characterization of hrp2+ gene which was isolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of hrp2+ gene showed striking evolutionary conservation among the SNF2 family of proteins. The transcript of hrp2+ gene was found to be a 4.7 kb as identified by Northern hybridization. To investigate the inducibility of hrp2+ gene, transcript levels were examined after treating the cells to various DNA damaging agents. The transcripts of hrp2+ were induced by UV-irradiation. But the transcripts were not induced by treatment of
Methylmethane sulfonate (MMS). These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of this gene. Hrp2 protein was purified near homogeneity by combination of affinity chromatography. We tested the purified Hrp2 protein for the helicase activity in an oligonucleotide release assay. However we were unable to detect any helicase activity associated with the Hrp2 protein, indicating that the helicase motifs in Hrp2 are merely indicators of a broader DNA-dependent ATPase activity.
Effect of chiro-inositol from Soybean on Reducing Hyperglycemia and Its Role for Nutraceutic Supplement for Insulin Resistance
Hong Yonggeun ; Pak Yunbae ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 197~201
DOI : 10.5352/JLS.2005.15.2.197
Studies with diabetic mammalian systems showed that chiro-inositol administration decreased blood glucose levels. We investigated which foodstuffs contain large amounts of chiro-inositol by surveying vegetables, edible plants and other staples in an effort to explore the nutritional or therapeutic supplements of chiro-inositol for diabetic patients. In the course of our investigation, we found that soybean and soybean derivatives have high chiro-inositol levels (upto 20 mg/g). The purified chiro-inositol from the soybean was then tested for reducing hyperglycemia by administrating the chiro-inositol in streptozotocin-treated diabetic rats. The results showed that the intragastric administration of 50 mg chiro-inositol/kg BW lowered hyperglycemia by
and that the effect was sustained for approximately 12 hr.
Characterization of Salmonella Adhesins Required for Colonization of Animals
Kim Young Hee ; Kim Sam Woong ; Kang Ho Young ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 202~210
DOI : 10.5352/JLS.2005.15.2.202
Following ingestion, Salmonella must adhere to and colonize the intestinal epithelium of the host in order to establish infection. S. typhimurium synthesize several appendages that are believed to mediate attachment. These include type 1 fimbriae, plasmid-encoded (PE) fimbriae, long polar (LP) fimbriae, and thin aggregative fimbriae. However, the precise roles of these putative adhesins remain unclear, due to conflicting data in the literature. We constructed strains lacking four different fimbriae including type 1 fimbriae, PE fimbriae, LP fimbriae, and thin aggregative fimbriae, as well as strains lacking each fimbriae alone. In cell culture adhesion assays, these mutants adhered to several mammalian cell lines as well as wild-type S. typhimurium. These strains were also screened for virulence in mice, and all strains were virulent or nearly as virulent as their wild-type parents. In contrast, When a strain lacking four fimbriae was screened for virulence in chicks, it was found to be highly attenuated. This suggests a role for either type 1 fimbriae, PE fimbriae, LP fimbriae or thin aggregative fimbriae or a combination of thease fimbriae in the colonization of chicks. It also suggests that differences exist with respect to the surface structures that mediate attachment of Salmonella in chicks as compared with mice.
Combining Ability Test of F
Generation by Diallel Cross in Kidney Bean
Kim Yong-Chul ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 211~214
DOI : 10.5352/JLS.2005.15.2.211
The studies were conducted to obtain basic informations on inheritance of some quantitative characters in kidney bean (Phaseolus vulgaris L.). Seven parents (Felibon, Renka, Processor, Kaboom,
, Local variety
and Local variety
hybrids of 21 crosses from a set of diallel cross among varieties were used to estimate combining ability for eight agronomic characters such as days to flowering, days to maturity, stem length, pod numbers per plant, pod length, grain numbers per plant, 100-grain weight and grain weight per plant. General combining ability (GCA) and specific combining ability (SCA) were significantly different among all characters, and values of GCA were greater than those of SCA in all characters except number of grains per plant. In effect of GCA, Felibon expressed high GCA effect for days to flowering and number of grains per plant. Local variety
showed high GCA effect for 100-grain weight and stem length. Local variety
appeared to high GCA effect for 100-grain weight and grain weight per plant. Processor expressed high GCA effect for days to maturity and grain weight per plant. Kaboom showed high GCA effect days to flowering and days to maturity.
appeared high GCA effect for all characters except for days to flowering, days to maturity and stem length. In the SCA effect, crosses of Felibonx Renka and Local variety
exhibited in high negative effect for days to flowering. Thus, these crosses were evaluated to useful for breeding early maturing variety. Crosses of breeding high yield variety were considered of
and Local variety
Evaluation of Safety with Gamgung-tang Using Ames and umu Assays
Shon Yun Hee ; Kim Cheorl Ho ; Nam Kyung Soo ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 215~219
DOI : 10.5352/JLS.2005.15.2.215
Gamgung-tang (GGT) that is included in Gamdu-tang (consists of Glycyrrhizae Radix, black beans) and Gunggui-tang(consists of Angelicae Radix and Cnidii Rhizoma) showed therapeutic effect of autoimmume thyroiditis in the previous reports. GGT was tested for the safety using Ames and umu gene expression mutagenicity tests. In Ames test, Salmonella typhimurium TA98 and TA100 were used to identify mutagenic property, and the number of histidine revertants was measured. In SOS umu test, Salmonella typhimurium TA1535 containing plasmid pSK1002 was used as a test strain, and we monitored the levels of umu operon expression by measuring the
activity. Mutagenic activity in any assays we tested was not found. After treating S-9 mixture with GGT, mutagenic activity was also not found. The results of this study suggested that there was no DNA damage and mutagenicity of GGT.
The Effects of One Year Exercise Program on Exercise Capacity & Cognitive Function in Male Patients with Dementia
Kwak Yi-Sub ; Um Sang-Yong ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 220~224
DOI : 10.5352/JLS.2005.15.2.220
The purpose of the present study is to investigate the effects of long-term exercise program on exercise capacity and cognitive function (MMSE) in male patients with dementia. 24 male patients were divided into two experimental groups : the exercise group (n=12) and the control group (n=12). The exercise group participated in regular exercise program for 12 months, and their exercise capacity (cardiopulmonary function, muscle strength, muscular endurance, flexibility, balance, agility) and MMSE (Mini-mental state examination) levels were evaluated at baseline (pre), after 6 months (mid) and after 12 months (post). The subjects carried on group-exercise of
minute a day,
times per week. Statistical techniques for data analysis was paired samples t-test. The level of statistical significance was
The results of this study were summarized as follows: In the exercise group, there were significant differences in cardiopulmonary function, muscle strength, muscular endurance and MMSE at the times of pre & mid and pre & post, there were significant differences in balance and agility at the time of pre & post only, whereas there was no significant difference in flexibility following the long-term exercise. In the control group, there were no significant differences in all the times. Based on the results of this particular study, one year exercise program increases on the cognitive function & exercise capacity in male patients with dementia.
Environmental and Biological Effects at Narodo, in the Southern Water of Korea, on Bloom of Ichthyotoxic Dinoflagellates Cochlodinium polykrikoides
Cho Eun Seob ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 225~230
DOI : 10.5352/JLS.2005.15.2.225
The aim of this study was to examine the fluctuation in phytoplankton assemblages with regarding to environmental conditions and nutrients, which were surveyed quarterly over the fours seasons (February, May, July, and October). In turn, an understanding of biological effects should provide insights into a wide range of initiated Cochlodinium blooms in Narodo. Sampling was carried out throughout 2001 on the coasts of Busan (St. 1), Yeosu (St. 2), Narodo (St. 3), Kohung (St. 4), and Kwangdo (St. 5). The maximum surface water temperature was recorded in July, and it ranged from 20 to
. Salinity showed no great variation, which maintained itself in the range of 29-34 psu. The maximum surface salinity was recorded in February, which was about 34 psu. The chlorophyll
concentration of the surface water ranged from 0.01 to
. The concentrations of
were persistently high from February to October; in particular, the peak was observed at St. 1 in February and May (0.15 and
, respectively), while it was detected at St. 2 in July and October (0.22 and
respectively). Similar trends to those for
were observed in the concentrations of
. In contrast to nitrogen, a distinct peak of
at St. 3, 4, and 5 was observed throughout year
except for October. At St. 1 encounter a peak of cell number of 30,000 and
, respectively, in July and October. During the period of this study, the majority of the taxa were diatoms. The dinoflagellates were rather abundant after February, in particular at St. 3, 4, and 5 which attained an abundance of
without marked fluctuation during the period of this study. At St. 3, the highest average cell width,
, was recorded: the highest cell length,
, was measured in July. Consequently, dinoflagellates bloom in July at Narodo influenced by warm water current are not only associated with a desirable development of cell morphometric characteristics, but also with the health growth of C. polykrikoides. During the period of this study, warm water currents caused an increased water temperature in Narodo, but did not change the amount of nutrients.
Suppression of Matrix Metalloproteinase-9 Expression of Flavonoids from Metasequoia glyptostroboides
Yang Jae-Young ; Lee Ho-Jae ; Kho Yung-Hee ; Kwon Byoung-Mok ; Chun Hyo Kon ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 231~235
DOI : 10.5352/JLS.2005.15.2.231
Matrix metalloproteinases (MMPs) are a family of structurally and functionally related zinc-dependent enzymes responsible for proteolytic degradation of extracellular matrix components such as base membrane or interstitial stroma. MMPs play an important role in a variety of physiological and pathological tissue remodeling processes, including wound healing, embryo implantation, tumor invasion and metastasis. Since MMP-9 (gelatinase B) has unique ability to cleave type IV collagen, gene expression of MMP-9 has been focused on as a pharmacological target. Flavonoids are a class of compounds that are widely spread in plants. In the coures of screening for the suppressors of MMP-9 gene expression from natural products, Metasequoia glyptostroboides was selected. Six flavonoids, sciadopitysin, isoginkgetin, bilobetin, 2,3-dihydrohinokiflavone, luteolin and apigenin were purified as suppressors of MMP-9 gene expression from M. glyptostroboides. The suppressing activity of the isolated flavinoids on the MMP-9 gene expression was measured by gelatin zymography and Nothern blot analysis.
The Effects of Solidago virga-aurea var. gigantea Miq. Root Extract on Bone Metabolism in Growth Period Rats
Lee Ji-Won ; Park Jung-Hyun ; Lee Hyo-Joo ; Lee In-Seon ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 236~241
DOI : 10.5352/JLS.2005.15.2.236
To investigate the bioactivities of Solidago virga-aurea var. gigantea Mig. Root (SVR), we studied the effect of a SVR methanol extract on the activity of bone metabolism. Spraque-Dawley three-week-old female rats were randomly assigned to groups as follows : non-supplemented rats and supplemented with SVR at 10, 50, 100 mg/kg bw/day. Every week determined weight gain and food intake, urine and blood examination of mineral content of calcium and phosphorus was performed each at experimental periods of 3 and 9 weeks respectively; bone mineral density and bone mineral content were also assayed. There were no significant differences in body weight or feed efficiency ratio levels. However, the biological value of calcium and phosphorus excretion in the group supplemented with SVR extract decreased significantly more than that in the group not supplemented with SVR extract. Also, spine BMD, femur BMC and pelvis BMC per weight were significantly greater on SVR extract supplemented groups than that of the control group. In conclusion, it might be expected that methanol extract of SVR does not impair the growth of rats and may improve bone metabolism in rats.
The Effects of Hijikia fusiforme on Serum Lipid Levels in Ovariectomized Rats
Kim Jeang-Yub ; Kim Mihyang ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 242~247
DOI : 10.5352/JLS.2005.15.2.242
The aim of this study was evaluated the effect of biological activity of Hijikia fusiforme (HF). To investigate the effects of biological activity of HF ethanol extracts, Sprague-Dawley female rats were randomly assigned to the following groups: sham-operated rats (Sham), ovariectomized control rats (OVX-control), ovariectomized rats supplemented ethanol extracts of HF at 50 mg/kg bw/day (OVX-HF50), ovariectomized rats supplemented ethanol extracts of HF at 200 mg/kg bw/day (OVX-HF200). HF ethanol extracts were orally supplemented at the level of 1 mL per day. The body weight gain, food intake and food efficiency ratio were not significantly different between groups. Although total-cholesterol and triglyceride were increased in the ovariectomized control, supplementation with the HF extracts decreased the levels. And HDL-cholesterol levels were significantly increased after supplementation with the HF extracts $(p<0.05)$. According to these results, we could known the effects biological activity of Hijikia fusiforme. Consequently, we expect the effects of Hijikia fusiforme extract on cardiovascular diseases aging coming from postmenopausal.
Characterization of the White Spot Syndrome Baculovirus (WSBV) Infection In Fresh Shrimp, Penaeus chinensis, Cultured in Korea
Heo Moon-Soo ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 248~252
DOI : 10.5352/JLS.2005.15.2.248
The virions of causative virus for white spot syndrome in cultured Fresh shrimp, Penaeus chinensis were rod-shaped, double envelope. An average size of the virion was 70 nm in diameter and
nm in length. Histopathological test of affected stomach, heart, and lymphoid organ revealed nuclear hypertrophy. Infectivity trials carried out by injection and feeding with purified virus revealed high cumulative mortality to healthy shrimp. The twenty one different protein species were detected in the analysis of virion. The length of total DNA from the purified virus particles were detected as a single band, double-stranded DNA molecule of approximately 114 kb.
Differential Intracellular Localization of Mitotic Centromere-associated Kinesin (MCAK) During Cell Cycle Progression in Human Jurkat T Cells
Jun Do Youn ; Rue Seok Woo ; Kim Su-Jung ; Kim Young Ho ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 253~260
DOI : 10.5352/JLS.2005.15.2.253
Mitotic centromere-associated kinesin (MCAK), which is a member of the Kin I (internal motor domain) subfamily of kinesin-related proteins, is known to play a role in mitotic segregation of chromosome during M phase of the cell cycle. In the present study, we have produced a rat polyclonal antibody using human MCAK (HsMCAK) expressed in E. coli as the antigen. The antibody specifically recognized the HsMCAK protein (81 kDa), and could detect its nuclear localization in human Jurkat T cells and 293T cells by Western blot analysis. The specific stage of the cell cycle was obtained through blocking by either hydroxyl urea or nocodazole and subsequent releasing from each blocking for 2, 4, and 7 h. While the protein level of HsMCAK reached a maximum level in the S phase with slight decline in the
phase, the electrophoretic mobility shift from
began to be induced in the late S phase and reached a maximum level in the
phase, and then it disappeared as the cells enter into the
phase. Immunocytochemical analysis revealed that HsMCAK protein localized to centrosome and nucleus at the interphase, whereas it appeared to localize to the spindle pole, centromere of the condensed mitotic DNA, spindle fiber, or midbody, depending on the specific stage of the M phase. These results demonstrate that a rat polyclonal antibody raised against recombinant HsMCAK expressed in E. coli specifically detects human MCAK, and indicate that the electrophoretic mobility shift from
, which may be associated with its differential intracellular localization during the cell cycle, fluctuates with a maximum level of the shift at the
Investigation of Conserved Genes in Microorganism
Lee Dong-Geun ; Lee Jae-Hwa ; Lee Sang-Hyeon ; Ha Bae-Jin ; Shim Doo-Hee ; Park Eun-Kyung ; Kim Jin-Wook ; Li Hua-Yue ; Nam Chun-Suk ; Kim Nam Young ; Lee Eo-Jin ; Back Jin-Wook ; Ha Jong-Myung ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 261~266
DOI : 10.5352/JLS.2005.15.2.261
To figure out conserved genes in 66 microbial species and measuring the degree of conservation, analyses based on COG (Clusters of Orthologous Groups of proteins) algorithm were applied. Sixty-six microbial genomes, including three eukaryotes, hold 63 conserved orthologs in common. The majority
of the conserved genes was related to translation, meaning the importance of protein in living creatures. Ribosomal protein S12 (COG0048) and L14 (COG0093) were more conserved genes than others from the distance value analysis. Phylogenetically related microbes grouped in genome analysis by average and standard deviation of 63 conserved genes. The 63 conserved genes, found in this research, would be useful in basic research and applied ones such as antibiotic development.
The Application of Nanoliposome Composed of Ceramide as an Anti-irritant in Cosmetics
Jo Byoung Kee ; Ahn Gi Woong ; Shin Bong Soo ; Jeong Ji Hean ; Park Hae-Ryong ; Hwang Yong-Il ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 267~272
DOI : 10.5352/JLS.2005.15.2.267
The objective of this study is to suggest the potentialities of nanoliposome composed of ceramide as an anti-irritant against various irritants used in cosmetics. Ceramides are major structural components of the epidermal permeability barrier, which is known to play an essential part in human physiology by not only preventing the loss of water from the body but also protecting the body from external physical, chemical, and microbial insults. According to the results, better effects on reinforcement of skin barrier function and anti-irritation were obtained with nanoliposome composed of ceramide than with dispersed ceramide. And, we performed in vitro skin penetration test using horizontal Franz diffusion cells with skin membrane prepared from hairless mouse to evaluate the influence of nanoliposome composed of ceramide on the skin penetration of lactic acid in formulations. From the results, we found that the anti-irritation effects of nanoliposome containing ceramide were due to reduced penetration rate of irritants. Conclusively, we could develop a new anti-irritation system and apply this nanoliposome composed of ceramide to the final cosmetic products successfully.
Genetic Variation of Alien Invasive Red Clover (Trifolium pratense) in Korea
Huh Man Kyu ; Chung Kyung-Tae ; Jeong Yong-Kee ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 273~278
DOI : 10.5352/JLS.2005.15.2.273
Trifolium pratense (red clover, Fabaceae) is a short-lived herbaceous species and the species is introduced from Europe or North America to Korea approximately 60 years ago. Allozyme variability was examined in populations representing this species. A high level of genetic variation was found in T. pratense populations. Ten of 19 loci
showed detectable polymorphism. Genetic diversity was 0.220. The sexual reproduction, high fecundity, and colonization process are proposed as possible factors contributing to high genetic diversity. Genetic diversity (0.220) was lower than that (0.285) of North American red clover, T. pratense. Korean populations of red clover may be founded by a small sample of larger or moderate populations. An indirect estimate of the number of migrants per generation (Nm = 4.20) indicated that gene flow was extensive among Korean populations of this species.
Antimicrobial Activities of Opuntia ficus-indica var. saboten Makino Methanol Extract
Kim Hae-Nam ; Kwon Do-Hoon ; Kim Hae-Yun ; Jun Hong-Ki ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 279~286
DOI : 10.5352/JLS.2005.15.2.279
The Opuntia ficus-indica var. saboten Makino (Cactus) is a tropical or subtropical plant, which is cultivated or grows naturally in Jeju island. It has been widely used as folk medicine for burned wound, edema and indigestion. In addition, its extract has been claimed to have several biological activities including anti-inflammation in oriental medicine. In this study, we examined the antimicrobial activities of the methanol extract of Opuntia ficus-indica var. saboten Makino. The extract showed a broad spectrum of antimicrobial activity against pathogenic bacteria, including antibiotics resistant bacteria (MRSA, R-P. aeruginosa, VRE) and Propionibacterium acnes, yeast, and fungi. The extract retained the activity after heat treatment for 15 min at
and after extended storage, up to 10 weeks storage period at
, also stably retained its activity. It showed a better inhibitoring effect to the growth of E. coli than sodium benzoate did it at the same concentration. Addition of various salts or metal ions did not affect on its antimicrobial activity. Therefore, the antimicrobial characteristics of the extract can be applicable as a natural preservative and an antimicrobial agent for bacterial disease.
Anti-inflammatory Activity of the Galactomannans Produced by a New Fungal Strain Trichoderma erinaceum DG-312
Joo Ji-Hoon ; Lim Jong-Min ; Koo Kwang-Bon ; Yun Jong-Won ; Choi Jang Won ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 287~292
DOI : 10.5352/JLS.2005.15.2.287
The crude galactomannans (GMs) were obtained from the culture of a newly isolated fungus Trichoderma erinaceum DG-312 and their anti-inflammatory activity was investigated in mice. The maximum concentrations of mycelial biomass and GMs reached 9.44 g/l, 2.72 g/l at day 3 in a 5-l stirred-tank bioreactor, respectively. The results of Sepharose CL-6B gel chromatography and compositional analysis revealed that the crude GMs contain heterogeneous polysaccharides consisting of
galactose. The GMs was shown to possess a significant anti-inflammatory activity against acetic acid-induced inflammatory mouse model in a dose-dependent manner, when mice were treated with 100 and 200 mg GMs/kg body weight. The inhibition in vascular permeability
and in writhing response
evidenced an anti-inflammatory activity of the GMs. The marked anti-inflammatory and writhing-lowering properties of the GMs suggest its potential therapeutic use.
Influence of Exogenous Abscisic Acid Concentration on the Coloration of 'Kyoho' Grapes.
Han Dong Hyeon ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 293~297
DOI : 10.5352/JLS.2005.15.2.293
This experiment was carried to investigate the optimum concentration of abscisic acid (ABA) treatment for enhancing fruit coloration of 'Kyoho' grapes. Cluster and berry weights showed a tendency that increased in proportion to concentration of ABA treatment, but were not significant in all treatments. Also, soluble solids and titratable acidity were not significant during fruit development in all treatments. L-phenylalanine ammonia-lyase (PAL) activity showed a tendency that decreased after rapidly increased in all treatments, and was the highest in 1000 mg/l ABA treatment at final harvest. Anthocyanin and total phenolics contents were high in proportion to ABA treatment concentration, and anthocyanin content in 1000 mg/l ABA treatment was 2.5 folds of that in control. Fructose and glucose as soluble sugars were detected, but sucrose was not detected. Both fructose and glucose contents increased during fruit development, but showed little difference in all treatments.
The Effects of Abscisic Acid Application Time and Times on Fruit Coloration of 'Kyoho' Grapes
Han Dong-Hyeon ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 298~303
DOI : 10.5352/JLS.2005.15.2.298
This experiment was carried to investigate the optimum time and times of abscisic acid (ABA) treatment for the coloration in 'Kyoho' grapes. The L-phenylalanine ammonia-lyase (PAL) enzyme activities were showed highly in both ABA treatments at veraison and 10 days after veraison. However, PAL enzyme was slightly higher in ABA treatment at 10 days after veraison than in that at veraison. Anthocyanin content showed a tendency that were increased during fruit development after veraison in all treatments, and was the highest in ABA treatment at 10 days after veraison. Fructose and glucose as soluble sugars were detected by HPLC and showed little differences in all treatments. In times of ABA treatment, PAL activity showed a tendency that decreased after increased in all treatments. PAL activity in 2 times treatment of ABA was higher than other treatments. Also, anthocyanin content was highest in 2 times treatment of ABA, as 5 folds of control and folds of 1 time treatment of ABA. Both fructose and glucose contents in all ABA treatments was slightly higher than control.
Effect of Herbicide Paraquat on NAD(H)-Redox-cycle
Kim Mi-Lim ; Choi Kyung-Ho ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 304~310
DOI : 10.5352/JLS.2005.15.2.304
This study was carried out to investigate the effect of herbicide paraquat (1,1-dimethyl-4,4-bipyridilium dichloride) on the electron transport system of the cell. When actively growing cells of bacteria were exposed to the 1.0 mM paraquat, more than
of the cells were killed at 0 hour. But specific activities of superoxide dismutase (SOD) were not changed at 0 hour of paraquat treatment. Oxido-reductions of NAD (H) by the suspension of bacterial membtane, rat mithochondria and NAD-dependent dehydrogenase were accelerated by paraquat treatment.
Effect of Herbicide Paraquat on Electron Donor and Acceptor
Kim Mi-Lim ; Choi Kyung-Ho ;
Journal of Life Science, volume 15, issue 2, 2005, Pages 311~315
DOI : 10.5352/JLS.2005.15.2.311
When paraquat was added to the bacterial membrane or mitochondrial suspension, the mixture turned dark blue, but the color was disappeared by aeration. The same phenomenon was seen when electrons were supplied to the paraquat. Blue color appeared from near the cathode, and then spreaded to whole transit system. Coloration was accelerated by addition of alkali, but the color was reduced by addition of acid or oxygen. Paraquat exhibited absorption at ultraviolet region by electron transfer at the concentrations as low as 1.0 mM which did not exert difficulty in showing color reaction. Paraquat caused the increase of the optical density at 340 nm by electron transit, and an aspect of that had a strong resemblance to NADH. The acute toxic action of paraquat seemes to depend on inhibition of energy metabolism cased by paraquat action of electron donor and acceptor.