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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 15, Issue 6 - Dec 2005
Volume 15, Issue 5 - Oct 2005
Volume 15, Issue 4 - Aug 2005
Volume 15, Issue 3 - Jun 2005
Volume 15, Issue 2 - Apr 2005
Volume 15, Issue 1 - Feb 2005
Selecting the target year
Expression of Paenibacillus macerans Cycloinulooligosaccharide Fructanotransferase in Saccharomyces cerevisiae
Kim Hyun-Chul ; Kim Jeong-Hyun ; Jeon Sung-Jong ; Choi Woo-Bong ; Nam Soo-Wan ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 317~322
DOI : 10.5352/JLS.2005.15.3.317
The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into an E. coli-yeast shuttle vector, pYES2.0, resulting in pYGECFTN. The plasmid pYGECFTN (8.6 kb) was introduced into Saccharomyces cerevisiae SEY2102 cells and then the transformants were selected on the synthetic defined media lacking uracil. The cft gene expression in yeast transformant was demonstrated by the analyses cyclofructan (CF) spots on thin-layer chromatogram. The recombinant CFTase was not secreted into the medium and localized in the periplasmic space. The production of CF was observed after 5 min of the enzymatic reaction with inulin. The optimun pH and temperature for CF production were found to be at pH 8.0 and
, respectively. Enzyme activity was stably maintained up to
. The CF was produced from all inulin sources and was most efficiently produced from dahlia tubers and Jerusalem artichokes.
Growth Inhibition of Human Hepatoma and Bladder Carcinoma Cells by DNA Topoisomerae Inhibitor β-lapachone
Choi Da Yean ; Lee Jae Il ; Chung Hyun Sup ; Seo Han Gyeol ; Woo Hyun Joo ; Choi Yung Hyun ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 323~331
DOI : 10.5352/JLS.2005.15.3.323
The objective of the present study was to investigate the effect of
, a quinone obtained from the bark of the lapacho tree (Tabebuia avellanedae) in South America, on the cell growth of human hepatoma (HepG2) and bladder (T24) carcinoma cells. Exposure of cancer cells to
resulted in growth inhibition, morphological changes and apoptosis in a concentration-dependent manner, which could be proved by MTT assay and flow cytometry analysis. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses revealed that
did not affect the levels of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21 (WAFl/CIPl) expression. However, the transcriptional factor Sp-l and proliferating cell nuclear antigen (PCNA) protein levels were significantly down-regulated by
in both cell lines. Moreover,
treatment caused a dose-dependent inhibition of the expression of telomere regulatory gene products such as human telomere reverse transcriptase (hTERT) and telomerase-associated protein-l (TEP-l). Taken together, these findings suggest that
-induced inhibition of human hepatoma and bladder carcinoma cell proliferation is associated with the induction of apoptotic cell death via modulation of several major growth regulatory gene products, and provide important new insights into the additional mechanisms of the anti-cancer activity of
Evaluation of Microbial Epoxide Hydrolase Activity Based on Colorimetric Assay Using 4-(p-nitrobenzyl) Pyridine
Kim Hee Sook ; Lee Eun Yeol ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 332~336
DOI : 10.5352/JLS.2005.15.3.332
Epoxide hydrolase activities of various microbial cells were analyzed by colorimetric assay based on alkylation of epoxides with 4-(p-nitrobenzyl)pyridine (NBP). The epoxide hydrolase activity was determined by measuring the decrease of color intensity at 560 nm due to the decrease of styrene oxide substrate by epoxide hydrolase-catalyzed hydrolysis reaction. The experimental conditions of NBP colorimetric assay were optimized for the efficient measurement of epoxide hydrolase activities from various microbial cells.
Affection of Citric Acid Production from Tapioca Alcoholic Distillery Waste by Using the Cell of Aspergillus niger
Lee Yong-Hee ; Lee Dong-Hwan ; Chung Kyung-Tae ; Suh Myung-Gyo ; Roh Jong-Su ; Lee Kook-Eui ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 337~343
DOI : 10.5352/JLS.2005.15.3.337
Tapioca alcoholic distillery waste was utilized as dual purposes to produce citric acid and to reduce the amount of waste to be treated. Primarily an attempt was made to optimize the process conditions by Aspergillus niger in shake bath. The effects of pH, temperature, nitrogen and phosphorus sources on citric acid production were investigated. Maximum concentration of citric acid was made at temperature of
and pH of 4.3, while maximum cell dry weight was obtained at
. The addition of methanol or ethanol to culture medium promoted citric acid production remarkably, but the addition of
and Manganese as mineral source decreased the acid production.
Neuronal Nitric Oxide Synthase-Immunoreactive Neurons In the Hamster Visual Cortex: Lack of Co-localization with Parvalbumin
Jin Mi-Joo ; Lee Jee-Eun ; Ye Eun-Ah ; Jeon Chang-Jin ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 344~351
DOI : 10.5352/JLS.2005.15.3.344
Nitric oxide (NO) and calcium-binding proteins occur in various types of cells in the central nervous system. They are important signaling and calcium buffering molecules, respectively. In the present study, using immunocytochemistry we examined the distribution and the co-localization pattern of neurons containing neuronal nitric oxide synthase (nNOS) and parvalbumin in the visual cortex of hamster. The overall number of parvalbumin-immunoreactive (IR) neurons was 17 times higher than that of the nNOS-IR neurons in the hamster visual cortex. The highest differences were found in layer V, where parvalbumin-IR neurons were 54.7 times more abundant than nNOS-IR neurons. Many nNOS- and parvalbumin-IR neurons were similar in size, shape, and manner of distribution in the visual cortex. However, two-color immunofluorescence revealed that no neurons in the hamster visual cortex expressed both nNOS and parvalbumin. The present results indicate that there are subtle species differences in the co-localization pattern between nNOS and calcium-binding proteins. The present results also suggest not only the heterogeneity and functional diversity of nNOS-IRneurons in the visual cortex, but also the importance of understanding animal diversity
Effects of Changes in Exercise Intensity on Resting Metabolic Rate (RMR) and Energy Expenditure in Young Men
Kawk Yi-Sub ; Jin Young-Wan ; Park Chan-Ho ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 352~358
DOI : 10.5352/JLS.2005.15.3.352
The purpose of this study was to examine the effect of various exercise intensity on Resting Metabolic Rate (RMR), excess post exercise energy expenditure (EPEE), and thyroid hormonal changes in trained (TR) and untrained (UT) people. The subject of the present study were divided into two groups and four periods: trained (TR; n=6) and untrained (UT; n=6) group. And the periods were divided as follows; Resting (R), Maximal (M), High intensity (H), and Low intensity (L). The percent body fat and RMR of all subjects were measured at every periods. The RMR was measured early in the morning following a 12-hour fast using MMX3B gas analyzer and blood sample were collected from the anticubital vein to investigate thyroid hormonal (T3, T4, Free T3, Free T4, & TSH) changes. All the RMR values were expressed as absolute value/BSA
. And We also analyzed mean energy expenditure for 30 minutes during and after different intensity exercise. There was significant difference in RMR among different intensity of exercise. in TR (p < .05) not in the UT group. however, there was no significant different percent body fat in TR and in UT group. In the energy expenditure, there was significant different between TR and UT in HEE (high intensity exercise energy expenditure), LEE (low intensity exercise energy expenditure), HEEPE (high intensity exercise energy expenditure post exercise) & LEEPE (low intensity exercise expenditure post exercise). In the hormonal level, there was significant different in T4 level in the TR group at H period and in T4, Free T3, & Free T4 levels in TR group at L period, however there was no significant different in the UT group. The present cross-sectional study was design to investigate the relationship between exercise intensity and RMR. The focus of this investigation was to compare RMR in aerobically trained (TR) and untrained (VI). The relationship among RMR, exercise intensity and percent body fat would best be investigated using MMX3B and body composition analyzer. Each subject completed measurement of percent body fat, RMR, hormone in the period of maximal oxygen uptake exercise (M), high intensity exercise (H), and low intensity exercise (L). From the results, Low intensity of exercise (L), there was a trend for an increased RMR (kcal/day) in the TR not for the UT. This is best explained not by the reduced percent body fat but by the highly induced energy expenditure (during exercise and post exercise energy expenditure) and increased T4, Free T3, and Free T4 hormonal levels in the low intensity exercise for the TR group.
Spatial Autocorrelation within Three Populations of Sasa borealis in Korea
Huh Man Kyu ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 359~364
DOI : 10.5352/JLS.2005.15.3.359
Spatial autocorrelation was applied to microgeographic variations of Sasa borealis populations in Korea. Separate counts of each type of join (combination of genotypes at a single locus) for each allele, and for each distance class of separation, were tested for significant deviation from random expectations by calculating the Standard Normal Deviation. Moran's I was significantly different from the expected value in 25 of 150 cases
. Seven of these values
were negative, indicating genetic dissimilarity among pairs of individuals in the ten distance classes. Populations of S. borealis are small in Korea, and are distributed with occasional cutting of seed-bearing stems used for sieves. Thus, artificial disturbance may contribute to the fact that the S. borealis population of Jirisan is unusual in lacking spatial genetic structure.
Vr-Wr Analysis of Yield Characters in Cotton
Choi Chu-Ho ; Lee Shin-Woo ; Lee Cheol-Ho ; Chun Hyun-Sik ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 365~373
DOI : 10.5352/JLS.2005.15.3.365
The quantitative inheritance of some yield characters in Gosyium spp was carried out by means of a
diallel cross. In this study, 45 combinations of
generations were genetically analyzed through 10 different cultivars diallel cross population of cotton (Gosyium spp) at an experimental field. The results of Vr-Wr graph analysis of six characters such as number of boll, boll weight, lint weight per boll, 100 seeds weight, fiber fineness and fiber length in those combinations by the Hayman's method were as follow: 1. The significant difference was observed from the genetic variance of all the examined characters. 2. On based the Vr-Wr graphical analysis,
showed a complete dominance in all the experimental characters except boll weight, lint weight per boll and fiber fineness, but the dominance degree and gene arrangement of
were somewhat different from those of
Optimization of Mannitol Fermentation by Leuconostoc mesenteroides sp. strain JFY
Yoo Sun Kyun ; Hur Sang Sun ; Song Suckhwan ; Kim Kyung Min ; Whang Kyung Sook ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 374~381
DOI : 10.5352/JLS.2005.15.3.374
The production of functional foods providing health benefit is one of the fast growing fields in the food industry. Mannitol as GRAS (generally recognized as safe) is a functional food. Mannitol is about
as sweet as sucrose and slowly and incompletely absorbed from the intestine, suppling only about one-half energy value of glucose. Commercially, the mannitol is synthesized by catalytic or electrochemical reduction of glucose. However, as strong demand for natural products increased, biological techniques have been developed for mannitol production. The object of this study was to determine the optimum conditions of mannitol fermentation by Leuconostoc mesenteroides sp. strain JFY isolated from fermented vegetables. The processes parameters such as pH, temperature, yeast extract concentration, and fructose concentration were optimized. The chosen ranges were 4.5 to 7.5 for pH, 22 to
for temperature, 0.05 to
for yeast extract. and 5 to 350 g/L for fructose. The mineral medium used consisted of 3.0g
per 1 liter of deionized water. The optimum values of pH, temperature, yeast extract, and fructose concentration were obtained at about pH 6.5, temperature
, yeast extract
and fructose 30g/L. At optimum condition, the production of mannitol amounted to 31.6g/l. We hope that these findings are of particular importance for industrial application of mannitol production.
Effect of Simulated Acid Rain on Antioxidants and Related Enzymes in Garden Balsam (Impatiens balsamina L.)
Kim Hak Yoon ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 382~386
DOI : 10.5352/JLS.2005.15.3.382
To investigate the effects of simulated acid rain (SAR) on growth and biochemical defense responses of plant, garden balsam (Impatiens balsamina L.) was subjected to four levels of SAR based on pH (5.6, 4.0, 3.0, 2.0) and placed in the growth chambers for 2 weeks. SAR drastically inhibited both height and dry weight of garden balsam. The content of total carotenoid was tended to decrease, but the level of malondialdehyde was significantly increased by SAR. As the pH levels decreased from 5.6 to 2.0, the content of dehydroascorbate and oxidized glutathione of the plant were significantly increased. The enzyme (superoxide dismutase, ascorbate peroxidase etc.) activities of the plant affected by SAR were increased as the pH decreased. The results indicate that garden balsam may receive oxidative stresses by the application of SAR and by which the plant growth can be significantly retarded. A biochemical protective mechanism might be activated to nullify the oxidative stresses generated through SAR.
Establishment of Quantitative Evaluation Method for Screening Testicular Toxicity in Rats: 2-Bromopropane as an Example
Cha Shin-Woo ; Bae Joo-Hyun ; Son Woo-Chan ; Shin Jin-Young ; Shin Dong-Ho ; Kim Sung-Ho ; Park Seung-Chun ; Kim Jong-Choon ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 387~396
DOI : 10.5352/JLS.2005.15.3.387
The aims of the study were to establish a short-term screening test for detecting testicular toxicity of chemicals in rats and to determine whether a 2-week administration period is sufficient to detect testicular toxicity of 2-bromopropane (2-BP) as an example. Male Sprague-Dawley rats were subcutaneously administered with 1000 mg/kg/day of 2-BP or its vehicle for 2 weeks. Ten male rats each were sacrificed on days 3, 7 and 14 after the initiation of treatment. Parameters of testicular toxicity included genital organ weights, testicular sperm head counts, epididymal sperm counts, motility and morphology, and qualitative and quantitative histopathologic examinations. The early histopathological changes observed on day 3 of treatment included degeneration of spermatogonia and spermatocytes, multinuclear giant cells, mature spermatid retention, vacuolization of Sertoli cells, and decreased number of spermatogonia in stages II and V. On day 7 of treatment, atrophy of seminiferous tubules, exfoliation of germ cells, degeneration of spermatogonia and spermatocytes, multinuclear giant cells, mature spermatid retention, vacuolization of Sertoli cells, decreased number of spermatogonia in stages II and V, and decreased number of spermatocytes in stages VII and XII. On day 14 after treatment, a significant decrease in the weights of testes and seminal vesicles was found. Atrophy of seminiferous tubules, exfoliation of germ cells, degeneration of spermatogonia and spermatocytes, mature spermatid retention, vacuolization of Sertoli cells, decreased number of spermatogonia in stages II and V, and decreased number of spermatocytes in all spermatogenic stages were also observed. In addition, a slight non-significant decrease in testicular sperm head counts, daily sperm production rate and epididymal sperm counts was found. The results showed that 2 weeks of treatment is sufficient to detect the adverse effects of 2-BP on male reproductive organs. It is considered that the short-term testicular toxicity study established in this study can be a useful tool for screening the testicular toxic potential of new drug candidates in rats.
Anti-proliferative Effects of the Isothiocyanate Sulforaphane on the Growth of Human Cervical Carcinoma HeLa Cells
Park Soung Young ; Bae Song-Ja ; Choi Yung Hyun ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 397~405
DOI : 10.5352/JLS.2005.15.3.397
Sulforaphane, an isothiocyanate derived from hydrolysis of glucoraphanin in broccoli and other cruciferous vegetables, was shown to induce phase II detoxification enzymes and inhibit chemically induced mammary tumors in rodents. Recently, sulforaphane is known to induce cell cycle arrest and apoptosis in human cancer cells, however its molecular mechanisms are poorly understood. In the present study, we demonstrated that sulforaphane acted to inhibit proliferation and induce morphological changes of human cervical carcinoma HeLa cells. Treatment of HeLa cells with
sulforaphane resulted in significant G2/M cell cycle arrest as determined by flow cytometry. Moreover,
sulforaphane significantly induced the population of sub-G1 cells (9.83 fold of control). This anti-proliferative effect of sulforaphane was accompanied by a marked inhibition of cyclin A and cyclin-dependent kinase (Cdk)4 protein and concomitant induction of Cdc2, Cdk inhibitor p16 and p21. However, sulforaphane did not affect the levels of cyelooxygenases and telomere-regulatory gene products. Although further studies are needed, the present work suggests that sulforaphane may be a potential chemoprevetive/ chemotherapeutic agent for the treatment of human cancer cells.
Expression of DNA-dependent Protein Kinase and Its Relationship with Epidermal Growth Factor Receptor Signaling in Metastatic Cancer Cell Lines
Hwang Jee Young ; Kim Sun Hee ; Kang Chi Dug ; Yoon Man Soo ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 406~414
DOI : 10.5352/JLS.2005.15.3.406
The genetic instability of cancer cells may be related to inappropriately activated DNA repair pathways. In present study, the modulated expression of DNA-dependent protein kinase (DNA-PK), a major DNA repair protein, in human cancer metastatic cells was tested. The expressions of Ku70/80, regulatory subunit of DNA-PK, and the Ku DNA-binding activity in various highly metastatic cell lines were higher than those in each parental cell line. Also, the expression of DNA-PKcs, catalytic subunit of DNA-PK, and the kinase activity of the whole DNA-PK complex in highly metastatic cells were significantly increased as compared to those of parental cells, suggesting that the enhanced DNA repair capacity of metastatic cells could be associated with aberrant use of DNA repair, which may mediate tumor progression and metastatic potential. Increased EGFR (epidermal growth factor receptor) signaling has been associated with tumor invasion and metastasis, and the linkage between EGFR-mediated signaling and DNA-PK has been suggested. This study showed that PKI166, the new EGFR tyrosine kinase inhibitor, modulated the expressions of Ku70/80 and DNA-PKcs and also revealed the chemosensitization effect of PKI166 against metastatic cells may be in part due to inhibition of Ku70/80. These results suggest that interference in EGFR signaling by EGFR inhibitor resulted in the impairment of DNA repair activity, and thus DNA-PK could be possible molecular targets for therapy against metastatic cancer cells.
Studies on the Function of the Rv2435c Gene of the Mycobacterium bovis BCG
Lee Seung-Sil ; Bae Young-Min ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 415~422
DOI : 10.5352/JLS.2005.15.3.415
library of the mutants was prepared by transposon mutagenesis of the Mycobacterium bovis BCG. We screened this library for the resistance to an anti-tuberculosis antibiotic, PA-824. Most of the mutants resistant to the PA-824 were not able to synthesize the coenzyme
which is normally produced by the wild type M. bovis BCG strains. HPLC analysis of the cellular extract showed that one of those mutants which lost the ability to synthesize
still produced F0. The insertion site of the transposon in this mutant was determined by an inverse PCR and the transposon was found to be inserted in the Rv2435c open reading frame (ORF). Rv2435c ORF is predicted to encode an 80.3 kDa protein. Rv2435c protein appears to be bound to the cytoplasmic membrane, its N-terminal present in the periplasm and C-terminal in the cytoplasm. The C-terminal portion of this protein is highly homologous with the adenylyl cyclases of both prokaryotes and eukaryotes. There are 15 ORFs which have homology with the class III AC proteins in the genome of the M. tuberculosis and M. bovis. Two of those, Rv1625c and Rv2435c, are highly homologous with the mammalian ACs. We cloned the cytoplasmic domain of the Rv2435c ORF and expressed it with six histidine residues attached on its C-terminal in Escherichia coli to find out if this protein is a genuine AC. Production of that protein in E. coli was proved by purifying the histidine-tagged protein by using the Ni-NTA resin. This protein, however, failed to complement the cya mutation in E. coli, indicating that this protein lacks the AC activity. All of the further attempts to convert this protein to a functional AC by a mutagenesis with UV or hydroxylamine, or construction of several different fusion proteins with Rv1625c failed. It is, therefore, possible that Rv2435c protein might affect the conversion of F0 to
not by synthesizing cAMP but by some other way.
Alkaloids from the Stem Bark of Phellodendron amurense Rupr
Lee Jin Hwan ; Lee Byong Won ; Kang Nam Suk ; Moon Yea Hwang ; Yang Min Suk ; Park Ki Hun ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 423~426
DOI : 10.5352/JLS.2005.15.3.423
Two isoquinolines and one quinolone were isolated from the stem bark of Pellodendron amurense Rupr. (Rutaceae). Two isoquinolines were elucidated as thalifoline (2) and pharmacological active berberine (3) has been blocking the release of
from internal stores. One quinolone was identified as N-methylatanine (1). This is the first report on the isolation of N-methylatanine (1) and thalifoline (2) from this plant.
Proteome Analysis of Drosophila melanogaster Used 2-DE and MALDI- TOF-MS
Park Jeong-Won ; Cha Jae-Young ; Song Jae-Young ; Kim Hee-Kyu ; Kim Beom-Kyu ; Jeon Beong-Sam ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 427~433
DOI : 10.5352/JLS.2005.15.3.427
With the completely discovery of the Drosophila genome sequence, the next great challenge is to extract its biological information by systematic expression and to perform functional analysis of the gene. Here we reported a proteome analysis of D. melanogaster with two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The cell extracts of D. melanogaster,
were resolved to more than 400 silver-stained spots by 2-DE. The most abundant protein spots were ranged from 4.0-7.5 of pI and from 15-90 kDa of molecular weight. The excised spots were destained and in-gel digested by trypsin. The masses of the resulting peptide mixtures were measured by MALDI-TOF-MS. Identified proteins were compared with measured peptide mass and a dynamic peptide searching database which is accessible via the internet. The results revealed that identified proteins were produced by 59 genes derived from 65 protein spots.
Characterization of a Fibrinolytic Enzyme from Pickled Anchovy
Yang Woong-Suk ; Lim Hak-Seob ; Chung Kyung Tae ; Kim Young-Hee ; Huh Man Kyu ; Choi Byung Tae ; Choi Yung Hyun ; Jeong Yong Kee ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 434~438
DOI : 10.5352/JLS.2005.15.3.434
In the previous study, we isolated a myulchikinase (MK), which has fibrinolytic activity and cytotoxicity to the tumor cell line, from myl- chi-jeot-gal. In this study, the effect of NaCl concentration, metallic ions, pH, temperature, and plasminogen on the activity of MK was analysed. The MK activity was maintained at least
activity up to
NaCl, which indicates that the enzyme may be halotolerant. The optimal pH and temperature were 8 and
, respectively. The fibrinolytic activity of MK was completely inhibited with 0.5 mM
and inhibited to
with 1 mM
. The MK showed strong activity in plasminogen- rich fibrin plate but not in plasminogen-free fibrin plate. The result indicates that the MK may be a plasminogen activator type fibrinolytic enzyme.
Characterization of Ferritin Isolated from Dog Spleen
Park Jae-Hag ; Jun Do Youn ; Kim Young Ho ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 439~446
DOI : 10.5352/JLS.2005.15.3.439
Ferritin is known to be the principle iron-storage protein in a wide variety of rganisms. The electrophoretic mobility and immunological cross-reactivity of dog splenic ferritin were compared with those of horse, bovine, and pig splenic ferritin after isolation using heat treatment, salting out, column chromatography, and ultrafiltration. These isolation methods allowed the recovery of
of the ferritin per g of spleen. The iron content in the dog ferritin was
, which appeared to be higher than those in the other mammalian ferritins tested. The electrophoretic mobility of the dog ferritin under nondenaturing conditions was similar to its bovine counterpart, whereas it was more identical to pig and horse ferritins on an SDS-polyacrylamide gel. The molecular weight of the dog ferritin subunit was 19.5 kDa on an SDS-polyacrylarnide gel, and the subunit was unable to bind with iron. The polyclonal anti-dog ferritin raised in rats was able to cross-react with the pig, bovine, and horse ferritins, upon Ouchterlony double immunodiffusiion. A Western blot analysis also revealed that the anti-dog ferritin, which specifically bound with the dog ferritin subunit, could also recognize the horse, bovine, and pig ferritin subunits and the maximum cross-reactivity was exhibited with the pig ferritin subunit, indicating that the dog ferritin is immunochemically more similar to the pig ferritin than its other mammalian counterparts. Accordingly, these results elucidate the biochemical and immunochemical characteristics of dog ferritin that might have a potential to be applied as an oral iron supplement to treat iron deficiency anemia.
Ecological Studies on the Vegetation of Chamaecyparis obtusa Community at Mt. Gyeryoung in Geojecity
Huh Man Kyu ; Choi Joo-Soo ; Moon Sung Gi ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 447~452
DOI : 10.5352/JLS.2005.15.3.447
The characters of Chamaecyparis obtusa community extended around the Mt. Gyeryoung in Geoje-cik were investigated for several ecological parameters and the results can be summarized as fellows. Chamaecyparis obtusa is prevailing in the plantation area, whereas Pinus densiflora and Quercus mongolica are prevailing in its outskirts. Plant biomass and net production which estimated from degree of green naturality and Miami's model were much higher in the plantation than those of the natural forest. However, the mean species diversity of plantation was lower than that of natural populations. In stratification of investigated areas, overstory tree layer was dominant in the zone of plantation and dominant layers in the natural forest were understory tree layer, shrub, and herb.
Cadmium Toxicity and Calcium Effect on Growth and Photosynthesis of Tobacco
Roh Kwang Soo ; Chin Hiw Seung ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 453~460
DOI : 10.5352/JLS.2005.15.3.453
This investigation was performed to study Cd toxicity and the influence of Ca on Cd toxicity in growth, and photosynthetic pigments and enzymes in tobacco. Cd inhibited both growth and level of chlorophyll, but the inhibition was compensated by the treatment of Ca. Especially, chlorophyll content was significantly increased by the combination of Cd and Ca treatment compared with Cd treatment alone. In addition, activity and content of rubisco by Cd treatment was also significantly lesser than the non-treated control. The highly reduced activity of rubisco was minimized by the combined treatment of Ca to Cd. Rubisco activase activity and content also showed a pattern of change similar to the rubisco level, suggesting that Cd- and Ca-induced changes of rubisco could be caused by rubisco activase.
Protective Effect of DWP-04 Against Hepatotoxicity Induced by D-galactosamine
Lee Jung-Hee ; Chi Sang Cheol ; Kim Seok-Hwan ; Shin Young-Ho ; Choi Jongwon ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 461~467
DOI : 10.5352/JLS.2005.15.3.461
This study was conducted to investigate the biological activity and hepatoprotective effect of DWP-04 [DDB : selenium yeast: glutathione (31.1 : 6.8 : 62.1(w/w/w)] in D-galactosamine (GaIN) intoxicated rats. The DWP-04 (50, 100 or 200 mg/kg) or its vehicle was orally administered everyday before the start of GaIN injection (400 mg/kg, ip) for two weeks and animal decapitated for 24 hrs after GaINinjected. The activities of serum enzymes, markers of liver function, were increased in the GaIN group compared to normal group and significantly lowered in the DWP-04 pretreated group than in the GaIN group. Hepatic lipid peroxide level and activities of phase 1 enzymes were significantly higher than those of GaIN group compared to normal group and lower in the DWP-04 pretreated group than in the GaIN group, and phase II enzyme activities in liver were lower in the GaIN group than in the normal group and were increased in the DWP-04 pretreated group than in the GaIN group. Total hepatic glutathione content and glutathione biosynthesis enzymes were lower in the GaIN group than in the normal group and were increased in the DWP-04 pretreated group than in the GaIN group. Therefore, the current results indicated that DWP-04 administration alleviated the GaIN-induced adverse effect through enhancing the antioxidant enzyme activities.
Effect of Salinity-stratified Waters on Upward Migration and Ratio of Extracted DNA/RNA in Cochlodinium polykrikoides Margalef Based on the Ratio of Absorbance at 260 and 280nm
Cho Eun Seob ; Lee Young Sik ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 468~473
DOI : 10.5352/JLS.2005.15.3.468
The coastal regions of Yeosu, the South Sea of Korea, has occurred annually the red tide which is caused by potentially ichthyotoxic dinoflagellate C. polykrikoides, with a wide avenue for exchange with oceanic waters and freshwater runoff from Sumjin river. We attempted to examine the variability in response to vertical migration and concentration of extracted DNA/RNA of C. polykrikoides exposed to salinity-stratified waters. The experimental aquarium of the 60 liter was employed to culture C. polykrikoides. One aquarium was supplied with only sea water, the other was consisted of sea water and freshwater. Experiment was conducted for 5 days. In experimental column (mixture of freshwater and sea water), salinity was maintained to 20 at upper and approximately 30 at bottom during the period of this study. The fluctuation with related to dissolved oxygen and pH was similar pattern to both columns. Chlorophyll a was significantly higher value at upper than bottom. During 24h, chlorphyll a on experimental column was extremely high on the top as soon as lighting, compared with control. With elapsed time, the gap between experimental and control columns was a little. In darkness, chlorophyll a was not significantly different between upper and bottom, most cells appeared to randomly distribute on column regardless of water layer. Fluctuation with related to concentration of extracted DNA and RNA based on ratio of absorbance of 260 and 280 nm in experimental column was higher at final day or diel migration than control. These results implied that a large volume of freshwater could be associated with influence of concentration of DNA and RNA, in particular, rapid upward movement caused massive fish kills as soon as sunset.
Development of Sequence Characterized Amplified Regions (SCAR) Showing for Cheju Native Horse
Cho Byung Wook ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 474~478
DOI : 10.5352/JLS.2005.15.3.474
This study was conducted to analyze genetic characteristics and to develop the specific marker for Cheju native horse (Coo) at the level of sequence characterized amplified regions (SCARs). We collected blood samples from Cheju native horse and Thoroughbred horse (Th) and obtained genomic DNA from the blood of 50 individuals randomly selected within the breeds. Seven hundred primers were chosen randomly and were used to examin the polymorphism and 40 kinds of primers showed polymorphic RAPD band patterns between two breeds. Thirty primers of them showed horse specific bands. With the primer MG 30, amplified band of 2.0 kb showed the specificity to Cheju native horse (Cnh). Additionally MG 53 detected the thoroughbred horse (Th) specific markers at size of 2.3 kb. As the next, 2.3 kb band from MG 53 was checked with the all individuals from all the breeds of this study, and it maintained the reproducible breed specificity to thoroughbred horse (Th). With this results, 2.3 kb band was cloned into plasmid vector and sequenced bidirectionally from both ends of the cloned fragment. With the obtained sequences 10 nucleotide extended primers including the original arbitray primer were designed as a SCARs primer. Finally, the primer with extended sequence showed the reproducible breed differentiation pattern and it was possible to identify Cheju native horse (Cnh) from other breeds. The SCARs marker 2.3 kb from MG 53 could be used to identify Cheju native horse (Cnh) for not only registration but also horse breeding programe.
Isolation of Mycoplasma pneumoniae and Antimicrobial Susceptibilities of the Isolates(III)
Chang Myung-Woong ; Kim Kwang-Hyuk ; Park In-Dal ; Song Gap-Young ; Kim Sung-Won ; Lee Eun-young ; Kim Moon-Chan ; Cho Myung-Hoon ; Kim Kyu-Earn ; Choi Choong-Eon ; Park Seon Yeong ; Jo Hyeon Jang ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 479~485
DOI : 10.5352/JLS.2005.15.3.479
The 994 throat swabs obtained from 688 adults and 306 children patients with respiratory diseases were examined for Mycoplasma pneumoniae infection by culture method. Antimicrobial susceptibilities of the resulting 123 M. pneumoniae isolates were evaluated by testing minimum inhibitory concentrations (MICs) of erythromycin, minocycline, tetracycline, josamycin, sparfloxacin, ofloxacin, and ciprofloxacin by a broth micro-dilution method. The erythromycin resistant strains of M. pneumoniae was determined above
of MIC for erythromycin. The erythromycin resistant strains of M. pneumoniae was confirmed resistant gene mutation of the portions of genes 23S rRNA (domain II and V), and ribosomal protein 14 and L22 by PCR amplified and their nucleotide sequenses were compared to those of the susceptible strain M129. The isolation rate of M. pneumoniae was
(89/688) for the adults and
(34/306) for the children. The
of the M. pneumoniae isolates were
for ciprofloxacin, ofloxacin, and tetracycline, respectively, and
for josamycin and erythromycin, respectively. The isolation rate of erythromycin resistant M. pneumoniae from patients was
for the adults,
for children, and
for the total. No mutation could be detected in the ribosomal protein L22 region, but all strains were mutated in the ribosomal protein L4 as two point mutation M144V. Two point mutations in domain V of 23S rRNA were selected in the presense of erythromycin resistant M. pneumoniae isolates, such as one strain was G2057C mutant, two strains were A2059C mutants, three strains were C2611G mutants, four strains were A2058C mutants, five strains were A2058T mutants, twenty strains were A2059G mutants, and twenty-five strains were A2058G mutants, respectively. These results show that erythromycin was not the most active compound against M. pneumoniae infection in Korea and clinical studies of macrolides in human patients are demanded.
Effects of Exercise on Axonal Regeneration and Growth-associated Protein (GAP43) Expression Following Sciatic Nerve Injury in Rats
Seo Tae-Beom ; Yoon Sung-Jin ; Kim Kyung-Tae ; Yoon Jae-Suk ; Yoon Jin-Hwan ; Park Sung-Tae ; Han In-Sun ; Namgung Uk ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 486~491
DOI : 10.5352/JLS.2005.15.3.486
Physical activity can improve sensorimotor recovery after peripheral nerve injury. Growth-associated protein 43 (GAP-43) is highly correlated with neuronal development and axonal regeneration and present in large quantities in the axonal growth cone. Using immunofluorescene staining and anterograde and retorgrade techniques, we identified enhanced axonal regrowth in distal stump of the sciatic nerve 3-14 days after crush injury in rats with treadmill training. We also carried out western blot to investigate GAP-43 protein expression in injured sciatic nerve. GAP-43 protein levels were highly induced in the injured sciatic nerve 3, 7 and 14 days compared with sedentary group. Thus, the present data provide a new evidence that treadmill training promoted axonal re-growth after injury and increased GAP-43 protein levels in the regenerating nerve.
Activation of Phospholipase D2 through Phosphorylation of Tyrosine-470 in Antigen-stimulated Mast Cells
Kim Young Mi ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 492~498
DOI : 10.5352/JLS.2005.15.3.492
The mechanism of activation of phospholipase D2 (PLD2) remains undefined although mechanisms have been described for the activation of PLDI. By expression of mutated forms of haemaglutinnin-tagged PLD2 in a mast cell (RBL-2H3) line, we show that PLD2 is phosphorylated at tyrosines -11, -14, and -470 and that tyrosine-470 is critical for activation of PLD2 by antigen. Studies were performed with mutated-DNA constructs for haemaglutinnin-tagged PLD2 in which codons for tyrosine -11, -14, -165, and -470 were mutated to phenylalanine either individually or collectively. Transient expression of these constructs showed that mutation of tyrosine -11, -14, -470, or all tyrosines (all-mutated PLD2) suppressed antigen-induced tyrosine phosphorylation of PLD2 but only the tyrosine-470 mutant failed to be activated by antigen as assessed by in vitro assay of immunoprepitated PLD2 or by assay of PLD in intact cells. The critical role of tyrosine-470 was confirmed in studies with add-back mutants (phenylalanine back to tyrosine) of the all-mutated PLD. The findings provide the first description of a mechanism of activation of PLD2 in a physiological setting.
Effect of Growth Retardants on Growth and Invertase Activity of Sedirea japonica Seedlings in vitro
Jee Sun Ok ; Cho Dong Hoon ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 499~504
DOI : 10.5352/JLS.2005.15.3.499
This experiment was conducted to identify the effect of several plant growth retardants on growth of Sedirea japonica seedlings cultured in vitro and their changes of invertase activities. When seedlings of Sedirea japonica were treated with ancymidol and paclobutrazol, as the concentrations were increased, leaf length was gradually shortened and leaf width became wider than that of control. On the other hand, root length was shorter, but the number of root and the root's diameters were greatly increased, compared with control. In 0.05mg/L uniconazole, growth of leaf and root were enhanced, compared with the control and higher concentrations of uniconazole. As concentration of each growth retardants was increased, leaf shape became round and smaller. Both soluble acid invertase activity and soluble alkaline invertase activity in leaf were decreased in higher concentrations of each growth retardant, but those of the root were contrary to those of the leaf. To confirm the estimated invertase activities, starch content of leaf was higher in low concentration treatments in each growth retardant, but in the root was contrary to content that of the leaf.
The Effect of Topiramate on Hippocampal Neuronal Death and Expression of Glutamate Receptor in Kainate-induced Status Epilepticus Model
Park Min-Jeong ; Ha Se-Un ; Bae Hae-Rahn ; Kim Sang-Ho ;
Journal of Life Science, volume 15, issue 3, 2005, Pages 505~512
DOI : 10.5352/JLS.2005.15.3.505
Excitotoxicity and epileptogenesis have often been associated with glutamate receptor activation. Accumulating evidences indicates that topiramate (TPM), an antiepileptic drug with multiple mechanisms of action has neuroprotective activity. We explored the neuroprotective effect of TPM on the status epilepticus (SE)-induced hippocampal neuronal death. After development of SE by kainite injection (15 mg/Kg), rats were treated with TPM (10mg/kg) for 1 week. The neuronal death was detected by Apop tag in situ detection kit, and the expression levels of glutamate receptors were semi-quantitatively analyzed by immunoblot. Kainate-induced SE caused a significant neuronal death and cell loss in CAI and CA3 regions of hippocampus at 1 week. However, treatment of TPM for 1 week after SE markedly reduced hippocampal neuronal death. The expression of N-methyl-D-aspartate (NMDA) receptor subunit 1, was increased by SE, but was not affected by 1 week treatment of TPM. The expressions of NMDA receptor subunit 2a and 2b were not changed by either SE or TPM. As for
(AMPA) glutamate receptors (GluR), kainate-induced SE markedly up-regulated GluR1 expression but down-regulated GluR2 expression, leading to increased formation of
permeable GluR2- lacking AMPA receptors. TPM administration for 1 week attenuated SE-induced expression of both the up-regulation of GluR1 and down-regulation of GluR2, reversing the ratio of GluR1/GluR2 to the control value. In conclusion, TPM protects neuronal cell death against glutamate induced excitotoxicity in kainate-induced SE model, supporting the potential of TPM as a neuroprotective agent.