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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 15, Issue 6 - Dec 2005
Volume 15, Issue 5 - Oct 2005
Volume 15, Issue 4 - Aug 2005
Volume 15, Issue 3 - Jun 2005
Volume 15, Issue 2 - Apr 2005
Volume 15, Issue 1 - Feb 2005
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Isolation and Characterization of Biosurfactant from Bacillus atrophaeus DYL,-130
Kim Sun-Hee ; Lee Sang-Cheol ; Park In-Hye ; Yoo Ju-Soon ; Joo Woo-Hong ; Hwang Cher-Won ; Choi Young-Lark ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 679~684
DOI : 10.5352/JLS.2005.15.5.679
The objective of this study was investigate the characteristic of biosurfactant produced from the iso-lated strain. The strain was isolated from soli samples of Duck-Yu Mountain and it was identified as Bacillus atrophaeus DYL-130 by 16S rDNA and gyrA gene nucleotide sequence analysis. The surface ten-sion of culture filtrate of Bacillus atrophaeus DYL-130 decreased to 28 mN/m and its biosurfactant con-centration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The emulsifying activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oils as substrate. The biosurfactant was purified by affinity chromatography and the surface activity of purified biosurfactant was measured by drop-collapsing method and it could be effectively emulsify toluene.
Effect of Solvent Fractions from Methanol Extract of Doenjang on Inhibition of Growth and DNA Synthesis of Human Cancer Cells.
LIM Sun-Young ; Rhee Sook-Hee ; Park Kun-Young ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 685~691
DOI : 10.5352/JLS.2005.15.5.685
Growth and DNA synthesis inhibitory effects of doenjang methanol extract and its solvent fractions on AGS human gastric adenocarcinoma cells, Hep 3B human hepatocellular carcinoma cells, HT-29 human colon cancer cells and MG-63 human osteosarcoma cells were studied. The treatment of doenjang methanol extract (
) with the AGS, Hep 3B, HT-29 and MG-63 cancer cells after 6 days of incubation inhibited the growth of cancer cells by
, respectively. To separate active compounds of doenjang, doenjang methanol extract was fractionated with dichloromethane, ethylacetate, and buthanol. Among the solvent fractions, the dichloromethane and ethylacetate fractions showed the highest growth inhibitory effects on various cancer cells. For example, the dichloromethane and ethylacetate fractions (
) sig-nificantly inhibited the growth of various cancer cells by
, respectively. DNA synthesis of AGS and Hep 3B cancer cells was significantly inhibited by adding dichloromethane fraction (
) up to
, respectively. Similarly, the ethylacetate fraction (
) showed a
inhibition rate of DNA synthesis in AGS cells. These results suggest that the dichloromethane and ethylacetate fractions have specific active compounds, which will explain this anticancer effect of doenjang.
Antifungal Activity of Extract from Xmthitim strumarium L. Against Plant Pathogenous fungi.
Park, Sung-Min ; Jung, Hyuck-Jun ; Han, Sun-Hee ; Yeo, Soo-Hwan ; Kim, Young-Won ; Ahn, Hyung-Geun ; Kim, Hyun-Soo ; Yu, Tae-Shick ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 692~695
DOI : 10.5352/JLS.2005.15.5.692
Antifungal activity of ether and ethylacetate extract from Xanthium strumarium L. were tested against 11 plant pathogens by agar diffusion method. Antifungal activity of the ether and ethylacetate extract showed strong antifungal activity against plant pathogenous fungi, i.e. Phytophthora capsici, Sclerotinia sclerotiorum and Aspergillus niger. The
of the ether extract against Sclerotinia sclerotiorum was determined
Antifungal activity of the ether extract from Xanthium strumarium L. showed Rf value
Water Quality Variation and Removal Characteristics of Poliovirus by Biological Activated Carbon (BAC) and Ozone Treatment Process in Nakdong River.
Jung Eun-Young ; Park Hong-Ki ; Lee You-Jung ; Jung Jong-moon ; Jung Mi-Eun ; Hong Yong-Ki ; Jang Kyoung-Lib ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 696~702
DOI : 10.5352/JLS.2005.15.5.696
Ozonation is a disinfection technique of harmful mi-crobes commonly used in the treatment of drinking water. And Biological Activated Carbon (BAC) treatment also provides numerous benefits for drinking water utilities, including removal of micro- pollutants, improved treatment processes. The multiful-stage ozonation and BAC play roles as effective methods for removing several materials in raw water. Water quality variation in Nak dong river and the removal efficiency of viruses by ozonation-BAC process were investigated on pilot scale. During the period of survey, most of water quality parameters including
were highly improved after passing through the BAC. The removal efficiency of poliovirus type III in water treatment process using pilot-plant,
of viruses were removed by pre-ozonation, sedimentation and sand filteration process,
were removed after in BAC filteration step. In the removal survey of viruses by ozonation, ap-proximately
or polioviruses were inactivated by ozone of 0.4 mg/l within 5 min. and
were inactivated by ozone of 0.8 mg/l over 10 min.
Combined Eeffect of Exercise and L-arginine Supplementation on Cardiovascular and Immune Responses in SHR
Kawk Yi-Sub ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 703~706
DOI : 10.5352/JLS.2005.15.5.703
The purpose of this study is to investigate the combined effect of L-arginine supplementation and regular physical exercise on HR, BP, eNOS and Macrophage activation using SHR. To examine the differences among HR, BP, eNOS, and Macrophage activity levels, normotensive Wistar-Kyoto rats were used as a control. Thirty two male rats (six weeks old) were divided into four groups; eight WKY control (WKYC), eight SHR control (SHRC), eight SHR supplemented with L-arginine (SHRA), and eight SHR trained and supplemented with L-arginine (SHRTA). Obtained results were as follows : In the heart and blood pressure, there was significant differences anong the four group (p<.05) compare to SHRC. In the eNOS levels, there was significant differences among the four groups (p<.05) compare to SHRC. In the macrophage activity, there was significant differences among the four groups (p<.05) compare to SHRC. In conclusion, For the SHRC group, the level of eNOS is higher than that of WKYC, and we can expect tissue damage caused by toxic free radical. However, this can be stabilized by the L-arginine supplementation and regular physical training. we can also conclude regular aerobic training decrease cardiovascular stress caused by stabled macrophage activity. Therefore, we can trace it is the effect of training in SHR.
Histochemical Study of the Intestinal Mucosubstances in Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis
Jeong Cil-Nam ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 707~714
DOI : 10.5352/JLS.2005.15.5.707
The conventional histochemical staining were used to study mucosubstances properties of intestinal striated border and goblet cells in four teleostean species, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis. The following methods were used; PAS, AB pH 2.5, AB pH 1.0, AB pH 2.5-PAS, AF pH 1.7-AB pH 2.5 and HID-AB pH 2.5 stains. The mucosubstances of striated border in the proximal intestine and rectum of Sebastes schlegeli contained with neutral mucin, middle and distal intestine contained with neutral mucin and acid mucin. The striated border of all the intestines of Halichoeres poecilopterus contained with neutral mucin and acid mucin, and those of Bryzoichthys lysimus and Tnkifugu pardalis contained with neutral mucin only. The amounts of neutral mucin were moderate to considerable in Sebastes schlegeli and Halichoeres poecilopterus, minimal to small in Bryzoichthys lysimus and Tnkifugu pardalis. The amounts and properties in mucosubstances of intestinal goblet cells showed differences in species and regions. The intestinal goblet cells of Bryzoichthys lysimus, and Tnkifugu pardalis contained neutral mucin only while Sehastes schlegeli and Halichoeres poecilopterus contained mixture of neutral mucin, sulfomucin and sialomucin. The amounts of neutral mucin were considerable to large in distal intestine and rectum of Tnkifugu pardalis, while moderate to considerable in all intestines of Sehastes schlegeli, all the intestines except for middle intestine of Bryzoichthys lysimus, and proximal and middle intestine of Tnkifugu pardalis. Also it was minimal to small in middle intestine of Halichoeres poecilopterus. The intestinal goblet cells of Sehastes schlegeli contained mixture of minimal amounts of strong sulfmucin, weak sulfomucin and minimal to small amounts of sialomucin, and those of Halichoeres poecilopterus except for rectum contained mixture of minimal to small amounts of strong sulfomucin and sialomucin.
Screening of Antibacterial Agent Against Streptococcus mutans from Natural and Medicinal Plants,
Kim Kun-Woo ; Baek Jueng-Kuy ; tang Young-Wook ; Kum Eun-Joo ; Kwon Yun-Suk ; Kim Hong-Ju ; Sohn Ho-Yong ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 715~725
DOI : 10.5352/JLS.2005.15.5.715
Oral streptococci are major constituents of dental plaques, and their prevalence is closely linked with various pathologic symptoms, such as dental caries. To develop natural anticaries agent, we prepared 309 kinds of plant extracts from 215 species of edible or medical plants, and antibacterial activity of the extracts against Streptococcus mutans JC-2 were evaluated based on 96 well microtiter plate assay and disk paper method, subsequently. Among the tested plant extracts, Ailanthus altissima, Paeonia lactiflora, Rubus phoenicolasius, Aralia continentalis, Quercus acutissima, Persicaria hydropiper and Agrimonia pilosa extracts showed strong antimicrobial activity. Determination of minimal inhibitory concentration (MIC) of the selected seven plant extracts showed that Ailanthus altissima, Persicaria hydropiper and Quercus acutissima extracts ($MIC
Modulacon of Cell Cycle Control by Histone Deacetylase Inhibitor Trichostatin A in A549 Human Non-small Cell Lung Cancer Cells
Hwang Ji Won ; Kim Young Min ; Hong Su Hyun ; Choi Byung Tae ; Lee Won Ho ; Choi Yung Hyun ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 726~733
DOI : 10.5352/JLS.2005.15.5.726
Histone deacetylase (HDAC) inhibitors target key steps of tumor development. They inhibit proliferation, induce differentiation and/or apoptotic cell death, and exhibit potent antimetastatic and antiangiogenic properties in cancer cells in vitro and in vivo. Although they are emerging as a promising new treatment strategy in malignancy, how they exert their effect on human non-small cell lung cancer cells is as yet unclear. The present study was undertaken to investiate the underlying mechanism of a HDAC inhibitor trichostatin A (TSA)-induced growth arrest and its effect on the cell cycle control gene products in a human lung carcinoma cell line A549. TSA treaoent induced the growth inhibition and morphological changes in a concentration-dependent manner. Treatment of A549 cells with TSA resulted in a concentration-dependent increased G1 (under 100 ng/ml) and/or G2/M (200 ng/ml) cell population of the cell cycle as determined by flow cytometry Moreover, 200 ng/ml TSA treatment significantly induced the population of sub-G1 cells (23.0 fold of control). This anti-proliferative effect of TSA was accompanied by a marked inhibition of cyclins, positive regulators of cell cycle progression, and cyclin-dependent kinases (Cdks) expression and concomitant induction of tumor suppressor p53 and Cdk inhibitors such as p21 and p27 Although further studies are needed, these findings provide important insights into the possible molecular mechanisms of the anti-cancer activity of TSA in human lung carcinoma cells.
Molecular Cloning and Nucleotide Sequence of Xylanase gene (xynT) from Bacillus alcalophilus AX2000.
Park Young-Seo ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 734~738
DOI : 10.5352/JLS.2005.15.5.734
A gene coding for xylanase from alkali-tolerant Bacillus alcalophilus AX2000 was cloned into Escherichia coli
using pUC19. Among 2,000 transformants, one transformant showed clear zone on the detection agar plate containing oat-spells xylan. Its recombinant plasmid, named pXTY99, was found to carry 7.0 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynT) was determined, xynT gene was found to consist of 1,020 base-pair open reading frame coding for a poly-peptide of 340 amino acids with a deduced molecular weight of 40 kDa. The coding sequence was preceded by a putative ribosome binding site, and the transcription initiation signals. The deduced amino acid sequence of xylanase is similar to those of the xylanases from Bacillus sp. Nl37 and B. stearothermophilus 21 with
identical residues, respectively
The Effect of Pyroligneous Liquor and Coconut Water on Plantlet Multiplication and in Vitro Flowering of Dendrobium moniliforme
Jee, Sun-Ok ; Cho, Dong-Hoon ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 739~742
DOI : 10.5352/JLS.2005.15.5.739
This experiment was carried out to clarify the effect of pyroligneous liquor and coconut water on plantlet multiplication and in vitro flowering of Dendrobium moniliforme. Plantlet growth and multiplication was good in 1.0 ml/L pyroligneous liquor treatment which was added to the basal media of 3 g/L hyponex and 4 g/L peptone (
) containing 0.1 mg/L NAA and 1.0 mg/L kinetin. In the treatment of 30 ml/L coconut water showed good results on plantlet growth and multiplication. In vitro flowering showed highest rate in the treatment of 1.0 ml/L pyroligneous liquor and 30 ml/L coconut water which were added to the basal media of
containing 0.1 mg/L NAA and 1.0 mg/L kinetin.
Isolation and Identification of a Histamine-degrading Barteria from Salted Mackerel
Hwang Su-Jung ; Kim Young-Man ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 743~748
DOI : 10.5352/JLS.2005.15.5.743
Histamine can be produced at early spoilage stage through decarboxylation of histidine in red-flesh fish by Proteus morganii, Hafnia alvei or Klebsiella pneumoniae. Allergic food poisoning is resulted from the histamine produced when the freshness of Mackerel degrades. Conversely it has been reported that there are bacteria which decompose histamine at the later stage. We isolated histamine decomposers from salted mackerel and studied the characteristics to help establish hygienic measure to prevent outbreak of salted mackerel food poisoning. All the samples were purchased through local supermarket. Histamine decomposers were isolated using restriction medium using histamine 10 species were selected. Identification of these isolates were carried out by the comparison of 16S rDNA partial sequence; as a result, we identified Pseudomonas putida strain RA2 and Halomonas marina, Uncultured Arctic sea ice bacterium clone ARKXV1/2-136, Halomonas venusta, Psychrobacter sp. HS5323, Pseudomonas putida KT2440, Rhodococcus erythropolis, Klebsiella terrigena (Raoultella terrigena), Alteromonadaceae bacterium T1, Shewanella massilia with homology of
respectively. Turbidometry determination method and enzymic method were employed to determine the ability of histamine decomposition. Among those species Shewanella massilia showed the highest in ability of histamine decomposition. From these results we confirmed various histamine decomposer were present in salted mackerel product in the market.
Identification and Cultural Characterization of Antioxidant Producing Bacteria Isolated from the Jeju Coasts
Kim Man-Chul ; Heo Moon-Soo ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 749~754
DOI : 10.5352/JLS.2005.15.5.749
An antioxidant- producing bacterium was isolated from sea water in Jeju island. The isolated strain, SC2-1 was gram-positive, catalase positive, facultatively anaerobic, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and sodium chloride was required for the bacteria growth. The radical scavenging activity of the culture supernatants was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This bacterium was identified based on cellular fatty acids analysis and 16S rDNA sequencing, and then named Exiguobacterium sp. SC2-1. The modified optimal medium compositions required the addition of maltose
, yeast extract
in marine broth (Difco. Co. USA). Antioxidant activity of under optimal culture conditions were
In vitro Synthesis of Ribonucleic Acids by T7 RNA Polymerase That was Fast Purified with a Modified Procedure
Kim Ki-Sun ; Choi Woo-Hyung ; Gong Soo-Jung ; Jeon Sung-Jong ; Kim Jae Hyun ; Oh Sangtaek ; Kim Dong-Eun ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 755~762
DOI : 10.5352/JLS.2005.15.5.755
Biochemical amounts of RNA molecules can be synthesized in vitro, which is functionally equivalent or similar to those transcripts normally existing at extremely low levels in vivo. In this study we described a method for efficient preparation of pure T7 RNA polymerase from Escherichia coli strain BL21/pAR1219. The procedure, which used ammonium sulfate fractionation and preparative column chromatography on sephadex SP, was shown to be simple, rapid, and cost effective in comparison with other methods reported previously, Using the purified T7 RNA polymerase we were able to synthesize very long RNA transcript of 1.54 kb length, which is not feasible by conventional chemical synthesis. RNA molecule that was also synthesized by the purified T7 RNA polymerase, such as hammerhead ribozyme, retained its biochemical activity by cleaving the target RNA successfully in vitro. Thus, the procedure shown in this study can be useful to synthesize any length of RNA molecules in vitro in a simple and cost effective way for a variety of purposes.
Effects of one Year Exercise on ADL & Lipid Profiles in Male Patients with Dementia
Um Sang-Yong ; Kwak Yi-Sub ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 763~766
DOI : 10.5352/JLS.2005.15.5.763
The purpose of this study was to investigate effects of regular exercise on ADL (activities of daily living) and lipid profiles (total cholesterol, triglyceride, HDL-C, LDL-C) in male patients with dementia. The subjects were consisted of 24 male patients with dementia, they were divided into two groups. the exercise group (EC, n
Study of Community Classification Camellia japonica Forest in the Korean Peninsula
Jin Young-Kue ; Kim In-Taek ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 767~771
DOI : 10.5352/JLS.2005.15.5.767
The Camellia japonica vegetation in the Korean Peninsula was investigated by the methology of the Z-M school. The synthesis table of Camellion japonicae were arranged for association classification from 263 quadrats. The vegetation of Camellia japonica forest, classfied as an alliance includes nine new associations : Camellietum japonicae typicum Jin et Kim 2005, Machilo thunbergii-Camellietum japonicae Jin et Kim 2005, Pino thunbergii-Camellietum japonicae Jin et Kim 2005, Castanopo cuspidatae var. sieboldii-Camellietum japonicae Jin et Kim 2005, Lito japonicae-Camellietum japonicae Jin et Kim 2005, Castnnopo cuspidatae var. thunbergii-Camellietum japonicae Jin et Kim 2005, Querco acutae-Camellietum japonicae Jin et Kim 2005, Neolito sericae-Camellietum japonicae Jin et Kim 2005, Cinnamomo japonicae-Camellietum Japonirae Jin et Kim 2005.
Biocatalysis and Biotransformation for the Production of Chiral Epoxides
Kim, Hee-Sook ; Lee, Ok-Kyung ; Lee, Eun-Yeol ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 772~778
DOI : 10.5352/JLS.2005.15.5.772
Chiral epoxides are important chiral synthons in organic synthesis for the production of chiral pharmaceuticals and functional food additives. Chiral epoxides can be synthesized by enantioselective introduction of oxygen to double bond of substrate by monooxygenase. Peroxidase also carry out asymmetric epoxidation of alkene in the presence of hydrogen peroxide. Kinetic resolution of racemic epoxides via enantioselective hydrolysis reaction by epoxide hydrolase (EH) is a very promising method since chiral epoxides with a high optical purity can be obtained from cheap and readily available racemic epoxides. In this review, various biocatalytic approaches for the production of chiral epoxides with several examples are presented and their commercial potential is discussed.
Assay of Epoxide Hydrolase Activity Based on PCR-linked in vitro Coupled Transcription and Translation System.
Lee, Ok-Kyung ; Kim, Hee-Sook ; Lee, Eun-Yeol ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 779~782
DOI : 10.5352/JLS.2005.15.5.779
Cell-free expression is a powerful tool for rapid protein analysis, enabling an efficient identification of gene without cumbersome procedure of transformation and cell culture. Epoxide hydrolase (EH) gene of Rhodotorula glutinis was simply amplified by PCR, and the resultant gene was expressed in vitro using a coupled Transcription/translation system. The cell-free expressed EH protein mixture exhibited the enantioselective hydrolysis activity toward (R)-styrene oxide, representing that cell-free protein synthesis system can be used for the rapid expression of an enantioselective enzyme for an efficient identification of the chiral activity.
Development of GOCHUJANG for Controlling V. parahaemolyticus with Green Tea and Natural Products
Kim Jong-Deog ; Kim Min-Yong ; Jung Sung-Ju ; Seo Hyo-Jin ; Kim Eun-Ok ; Lee Se-Yeong ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 783~789
DOI : 10.5352/JLS.2005.15.5.783
Vibriocidal GOCHUJANG was manufactured using extract of natural products and green tea. Antioxidative capacity of natural products and combination of natural products was measured with Oxidation-Reduction Potential (ORP) system value. Inhibitory ability of vibriocidal GOCHUJANG was compared with Inhibition Zone Diameter (IZD) values. Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george and Acanthodanacis cortex and combination of these natural products were showed higher antioxidant capacity and inhibitory effect against V. para-haemolyticus. Two kinds of combinations, A combination was composed of Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george, and B combination was consisted of Acanthodanacis cortex and A combination, were presented higher inhibition ratio with IZD values as
, respectively. Vibriocidal GOCHUJANG was prepared based on A and B combination with their higher IZD Value as
, respectively. When vinegar was added to A and B based GOCHUJANG, their IZD value was more higher as
, respectively B combination based GOCHUJANG was exhibited higher inhibitory effect than A based GOCHUJANG. Combination of natural products was more important process for increasing vibriocidal effect. But addition of soybean malt or chitosan or a garlic into A or B combined GOCHUJANG, no different effect was exhibited.
Effects of Liquid Culture of Coriolus versicolor on Lipid Metabolism and Enzyme Activities in Rats fed Cholesterol Diet.
Koh Jin-Bog ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 790~795
DOI : 10.5352/JLS.2005.15.5.790
The effects of liquid culture of Coriolus (C) versicolor on weight gain, food intakes, food efficiency ratios, serum and hepatic lipid concentrations were investigated in male rats fed the high cholesterol diets. Eight weeks old Sprague-Dawley rats were given three different types of diet for six weeks, respectively: a control diet (
cholesterol), two kinds of C versicolor diet (control diet +
C. versicozor in water) according to the levels of C. versicolor supplementation. The body weight gains of the rats fed
C. versicolor diets were lower than those in the rats fed the control diet. The food intake, food efficiency ratios, and liver, kidney, epididymal fat pad weights of the rats fed
C. versicoEer diets were similar to those of the rats fed the control diet. The concentrations of hepatic cholesterol and triglyceride in the rats fed
C. versicolor diets were significantly lower than those in the rats fed the control diet. The concentrations in serum total cholesterol, LDL-cholesterol, and atherogenic index ratios were significantly lower in the rats fed
C. versicolor diets compared to those fed the control diet. The HDL-cholesterol/total-cholesterol ratios was significantly higher in the rats fed
C. versicolor diets compared to those fed the control diet. The fecal excretion of total lipid and triglyceride in the rats fed
C. versicolor diet was significantly higher than that of the rats fed the control diet. There were no significant difference found in the serum trigly-ceride, phospholipid and HDL-cholesterol concentrations among the experimental groups. These results showed that the C. versicolor feeding decreased the hepatic cholesterol, triglyceride, and the serum total cholesterol, LDL-cholesterol and atherogenic index, and increased the serum HDL-cholesterol/ total-cholesterol ratio of the rats.
Characterization of Antioxidants Extracted from Leaves of Sanjook(Sasa boreails var. chiisanensis)
Yoo Mi-Young ; Park Sung-Hee ; Kang Young-Mo ; Yang Ji-Young ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 796~801
DOI : 10.5352/JLS.2005.15.5.796
For usage of natural antioxidants, sanjook (Sasa boreails var. chiisanensis) leaves were extracted with methanol and investigated about its antioxidative activities and stability. It showed that the antioxidant activity of methanol extracts from the leaves of sanjook depend on their concentration within range of 0.1 to 0.8 mg/ml. The methanol extracts from the leaves of sanjook represented
of DPPH radical scavenging ability,
of SOD-like activity and
scavenging ability, while BHT, as a compared substance, was
of DPPH radical scavenging ability,
of SOD-like activity and
scavenging ability, respectively. The anti-au-toxidation effect for methanol extracts from the leaves of sajook was
within range of 0.1 to 0.8 mg/ml. The pH stability on methanol extracts from the leaves of sanjook was most stable at pH 6. The more acid or akali it became, the more unstable it turned. The thermostability on methanol extracts from the leaves of sanjook remained above
of their DPPH activity at range of
Silencing of Disabled-2 Gene by CpG Methylation in Human Breast Cancer Cell Line, MDA MB-231 Cells
Ko Myung Hyun ; Oh Yu Mi ; Park Jun Ho ; Jeon Byung Hoon ; Han Dong Min ; Kim Won Sin ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 802~808
DOI : 10.5352/JLS.2005.15.5.802
Human Disabled-2 (Dab2) is a candidate tumor suppressor gone that regulates cell growth by c-Fos suppression in normal cells. In many cancer cells, Dab2 expression is lost or greatly diminished in
of the breast and ovarian cancers. In this study, we have examined the methylation status of CpG island on Dab2 gene promoter using bisulfite-assisted genomic sequencing and methylation specific PCR (MSP) method in human breast cancer cell line, MDA MB-231 cells. In normal human uterus endometrial cells, Dab2 was completely unmethylated. In contrast, Dab2 was methylated on CpG dinucleotides near the TATA_ box in MDA MB-231 cells. following MDA MB-231 cells by treatment with 5-azacytidine, Dab2 gene were demethylated and reexpressed. Result of this study suggested that silencing of Dab2 gene is correlated to CpG island methylation in human breast cancer cell line, MBA MD-231 cells.
Hypoglycemic and Antioxidative Effects of Fermented Chaga Mushroom (Inonotus obliquus) on Streptozotocin-induced Diabetic Rats
Cha Jae-Young ; Jun Bang-Sil ; Lee Chi-Hyeoung ; Yooi Ki-Soo ; Moon Jae-Chul ; Cho Young-Su ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 809~818
DOI : 10.5352/JLS.2005.15.5.809
The effects of fermented chaga mushroom (Inonotus obliquus) on the concentrations of serum glucose, insulin, lipids and lipid peroxidation in streptozotocin (STZ)-induced diabetic rats were investigated. Rats were fed a semisynthetic diet supplemented with 50 g/kg chaga mushroom powder (the CM group) and fermented chaga mushroom powder (the FCM group), and no supplemented (the control group) for 3 weeks. The polysaccharide concentrations were CM by
and FCM by
, and the total polyphenol concentrations were CM by
and FCM by
. Feed intakes and water consumption, serum glucose, insulin, triglyceride, and blood urea nitrogen concentrations were significantly lower in the FCM group than in both the CM and control groups. The activities of AST and ALT were also significantly lower in the FCM group than in the control group. No significant differences were detected with regard to the serum cholesterol and creatinine concentrations among the experimental groups. Lipid peroxidations in hepatic homogenate, microsomal and mitochondrial subcellular and pancreas were significantly lowered by the administration of FCM in the STZ-diabetic rats. Hepatic glutathione concentrations, which is closely associated with antioxidant system, was significantly higher in the FCM group than in the control group, indicating a marked effect of FCM administration on the endogenous antioxidant system. However, CM treatment showed a moderate antioxidative activity in the STZ-diabetic rats. Our results indicate that fermented chaga mushroom exert hypoglycemic and antioxidative effects in type 1 diabetes mellitus.
The Antioxidative Activity of Glutathione-Enriched Extract from Saccharomyces cerevisiae FF-8 in In Vitro Model System
Lee Chi-Hyeoung ; Cha Jae-Young ; Jun Bang-Sil ; Lee Ho-Jun ; Lee Young-Chun ; Cho Yong-Lark ; Cho Young-Su ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 819~825
DOI : 10.5352/JLS.2005.15.5.819
The Antioxidative accvities of the cell free extracts containing high glutathione by Saccharomyces cerevisiae FF-8 were tested in vitro experimental models : DPPH method for radical scavenging activity, ferric TBA method and ferric thiocyanate method using linoleic acid and tissue microsome for lipid peroxidation inhibitions. The concentration of intercellular glutathione by cultivating S. cerevisiae FF-8 in the YM optimal medium obtained
, which was increased by 2.76-fold from
in the YM basal medium. A comparition between the YM basal medium and the YM optimal medium on antioxidative substance produced by S. cerevisiae FF-8 was investigated. In DPPH (
) method, the electron donating activity of the glutathione produced by S. cerevisiae FF-8 cultured in the YM optimal medium was as high as that of BHT (
). The antioxidative a.tivity was measured by inhibition against lipid peroxidation of rat tissues` microsomes. The results of anti-oxidant activity of the cell free extracts by S. rerevisiae FF-8 cultured in the YM optimal medium was shown in the following order . $ liver 60.98\% > kidney 56.43\% > heart 52.91\% > brain 52.13\% > testis 45.57\% > spleen 42.95\% $. In antioxidative activities determined by ferric thiocyanate method and TBA methods against lipid peroxidation, the lipid peroxidation in the control mixture increased more rapidly than the typical peroxidation curve of linoleic acid from one day. The antioxidative activity of the cell free extracts by cultivating S. cerevisine FF-8 in the YM optimal medium were higher than that of the YM basal medium. These data indicate that the cell free extracts containing a high intercellular glutathione of S. cerevisiae FF-8 cultured in YM optimal medium showed strong antioxidative capacities by DPPH radical scavenging activity and ferric thiocyanate and TBARS measurements
Concentration of Zn, Cu, and Pb in Soils and Accumulation of Its in Plants around Abandoned Mine Vicinity
Seo Sang-Woo ; Moon Sung-Gi ; Choi Chul-Mann ; Park Yeon-Kyu ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 826~833
DOI : 10.5352/JLS.2005.15.5.826
This study was conducted to inform basic data for management of abandoned mine that was based on soil heavy metal concentration of the non-rearing and the rearing sites in Guundong Mine which had been completed of improve contamination soils, and examined into standard plant which had tolerances about pollution that analyzed into its accumulated heavy metals contents in the wild plant of naked soil. Consider form the result of contents by depth of soils heavy metals in the non-rearing sites, S1 point judged by contaminated to heavy metals of tailing leach after weathering caused by rainfall and permeate in S1 point soils when restored rest of tailing in 1996 restoration project. Lead of S2 point was not contaminated seriously by restoration and S3 point considered that heavy metals which leach by rainfall in the contaminated soils in mine vicinity accumulated in the abandoned paddy through river When heavy metals of rearing sites soils were examined from the view point of upside of mine point (SP1, SP2, and SP3) of same above the sea level, zinc (537.5 mg/kg) content of SP1 point was highest, and copper (535 mg/kg) and lead (141.5 mg/kg) in the SP3 point were high. To considered as orderly plenty of heavy metals in the plant were respectively in order, Perilla frutescens var. cauta KUDO (SP1 and SP4) >Artemisia princeps PAMPAN (SP2) > Miscanthus sinensis ANDERSSON(SP5) > Vicia angustifolia var. segetilis (THUILL.) K. KOCH. (SP2). Especially, there were plenty of heavy metals in P. frutescens var. acuta, so it judged as a standard plant which have tolerances about heavy metals pollution sites. Therefore, we need more study about the heavy metals accumulate ability of P frutescens var. acuta in future.
Effects of Mulberry fruits Extracts on Serum Lipid Level in Ovariectomized Rats
Choi Kyung-Ha ; Park Mi-Hwa ; Kim Mihyang ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 834~839
DOI : 10.5352/JLS.2005.15.5.834
The aim of this study was to evaluate the effects of three different mulberry cultivars extracts on serum lipid contents in ovariectomized estrogen-deficient rats. Sprague-Dawley female rats were randomly assigned to the following groups: sham-operated rats (sham), ovariectomized control rats (OVX-control), ovariectomized rats supplemented with
ethyl alcohol extracts from various kinds of Tajikistan mulberry (OVX-TM), Korea mulberry (OVX-KM) and China mulberry (OVX-CM) at 200mg/kg bw/day, respectively. The mulberry extracts were orally administrated at 1mL per day. The body weights of OVX rats were significantly heavier than the sham-operated rats at all values (p <0.05). The ovariectomy caused an expected increasing in the levels of serum total cholesterol and triglyceride. The serum HDL-cholesterol level on the OVX-TM, OVX-KM and OVX-CM groups were higher than in the OVX-control group
Effects of Cooking Method and Temperature on the Lipid Oxidation of Electron-Beam Irradiated Hanwoo Steak.
Park T. S. ; Shin T. S. ; Lee J. I. ; Park G. B. ;
Journal of Life Science, volume 15, issue 5, 2005, Pages 840~846
DOI : 10.5352/JLS.2005.15.5.840
This study was carried out to investigate the effect of electron beam irradiation and cooking temperature on physico-chemical characteristics and lipid oxidation of beef. A total of six beef carcasses (
) that were quality grade
(marbling score No.7, meat color No.4, maturity No.1, texture No.1) was purchased at the commercial slaughter house. The carcasses were transported and washed using high pressure water, and pasteulized with
ethyl alcohol in the laboratory. After the carcasses were deboned and trimmed, loin and round were taken out to make steak (1.5cm thickness) or ground beef respectively. Samples were wrap or vacuum packaged and irradiated with 0, 3, 4.5, 6 and 7.5 kGy using electron-beam accelerator at Samsung Heavy Industries Ltd. Co. (in Taejun). Irradiated samples were cooked with different methods(electronic pan and gas oven) and temperatures (
) and used to measure fatty acid composition, TBARS, cholesterol oxide products and panel test scores. The content of saturated fatty acids increased by increasing heating temperature in oven boiling steak (OBS) and pan boiling steak (PBS), and there was no difference by electron-beam irradiation. Both irradiated and non-irradiated treatment were high as the heating temperature increased in TBARS by heating temperature in PBS (p < 0.05) and the amount of Malonaldehyde (MA), standard of fat deterioration, was increased in OBS (p < 0.05). Non-irradiated and 3, 6 kGy treatment produced about 2 fold amount of MA at
. In comparison with PBS, OBS produced much amount of MA and a bit different from non-irradiated treatment but did not show no tendency. As irradiation levels and heating temperature increased, the amount of cholesterol oxides products was increased and also pan-heating method, direct heating method, significantly increased the degree of oxidation compared with oven-heating method, indirect heating method (p < 0.05).