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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 16, Issue 7 - Dec 2006
Volume 16, Issue 6 - Oct 2006
Volume 16, Issue 5 - Aug 2006
Volume 16, Issue 4 - Jul 2006
Volume 16, Issue 3 - Jun 2006
Volume 16, Issue 2 - Apr 2006
Volume 16, Issue 1 - Feb 2006
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Effects of the Feral Peach (Prunus persica Batsch var. davidiana Max) Extract on the Lipid Compositions and Blood Pressure Level in Spontaneously Hypertensive Rats
Kim, Han-Soo ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1071~1079
DOI : 10.5352/JLS.2006.16.7.1071
The purpose of this study was to observe the effects of the feeding physiological activity substance in feral peach(Prunus Persica Batsch var. davidiana Max.) extract intake on the improvement of the lipid compositions, apolipoprotein and blood pressure level in spontaneously hypertensive rats(SHR, Wistar strain, male) fed the experimental diets for 33 days. Concentrations of total cholesterol, triglyceride(TG), LDL-cholesterol, free cholesterol and atherosclerotic index in serum were significantly lower in the feral peach extract intake groups[groups 5g% Ex.(basal diet+feral peach 5.0g% extract), 10g% Ex.(basal diet+feral peach 10.0g% extract)] than those in the group Control(basal diet+water). In the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration, feral peach 5.0g%, 10.0g% extract intake groups(group 5g% Ex. and 10g% Ex.) were higher percentage than in the group Control. However, concentrations of total cholesterol and TC in liver and brain were significantly lower in the groups 5g% Ex. and 10g% Ex. than those in the group Control. But the concentrations of apolipoprotein (Apo) A-I and Apo A-II in serum were significantly higher in the feral peach 5.0g% and 10.0g% extract intake groups(5g% Ex. and 10g% Ex.) than in the control group. However, concentrations of Apo C-II, Apo C-III, Apo E and ratio of Apo B to Apo A-I in serum were fairly reduced in the groups 5g% Ex. and 10g% Ex. than in the control group. The levels of systolic and diastolic blood pressure were significantly lower in feral peach 5g% Ex. and 10g% Ex. groups than control group. However, no significance was found in the effect of among the groups(groups 5g% Ex. and 10g% Ex.). From these results, physiological activity substance in feral peach(Prunus persica Batsch var. davidiana Max.) extracts were effective on the improvement of the lipid compositions and cardiovascular heart disease, hypertension in spontaneously hypertensive rats. And particularly, feral peach extracts were more effective as a therapeutic regimen for the control of blood pressure in hypertension.
Inhibitory Effects of Solvent Extracts from Seven Brown Algae on Mutagenicity and Growth of Human Cancer Cells
Choi, Hyung-Ju ; Kil, Jeung-Ha ; Bak, Soon-Sun ; Kong, Chang-Suk ; Park, Kun-Young ; Seo, Young-Wan ; Lim, Sun-Young ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1080~1086
DOI : 10.5352/JLS.2006.16.7.1080
Hematological Characterization of Penaeus chinensis by Feed-additives
Kim, Yeong-Jin ; Lee, Sun-Yi ; Cho, Hyo-Jin ; You, Sun-Nyoung ; Kim, Kwang-Youn ; Choi, Won-Chul ; Heo, Moon-Soo ; Ahn, Soon-Cheol ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1087~1089
DOI : 10.5352/JLS.2006.16.7.1087
Shrimp has efficient non-specific defense mechanisms based on activities of the hemocytes against pathogens. Up to now, it has been known that one of the non-specific immune reactions is related to mutual association among types of hemocyte, granular cell, semi-granular cell, and hyaline cell. In this study, we tried to know the effectiveness of feed-additives such as 5-aminolevulinic acid(ALA), chitosan, and hot-water extract of herb on immunity of shrimp (Penaeus chinensis) by hemocytic observation and SDS-PAGE analysis. Finally, we suggest a principle of the examination system for effects of various feed-additives
The Effect of Phellinus linteus and Cordceps militaris Supplementation on Blood Fatigue Element Changes and Antioxidant System During Exercise
Paik, Il-Young ; Han, Dae-Seok ; Park, Tae-Sun ; Kwak, Yi-Sub ; Suh, Sang-Hoon ; Jin, Hwa-Eun ; Kim, Young-Il ; Woo, Jin-Hee ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1090~1096
DOI : 10.5352/JLS.2006.16.7.1090
The purpose of this study was to investigate the effects of p. Linteus and C. militaris supplement on lactate, phosphorous, ammonia, MDA(malondialdehyde), GPX(glutathione peroxidase) and TAS(total antioxidant status) following
, and 85%
exercise. The 15 male college students were divided into three groups: 5 P. liteus supplied(PL), 5 C. militaris supplied(CM), and 5 placebos supplied(PB) and compared the differences between supplement before and after. Obtained results were as follows: In the exercise performance time, there were no differences in PL, CM and PB groups. Regular supplement of P. linteus or C. militaris partially reduces fatigue induction factors. Also, P. linteus or C. militaris supplement decreases MDA, increases GPX and TAS. Therefore, it can be concluded that P. linteus or C. militaris supplement not only have a pharmacological effect for clinical treatment, but also have a maintenance effects on the tissue oxidant-antioxidant system after exercise.
Plant Succession and Changes on Community at Flooding Area
Huh, Man-Kyu ; Choi, Joo-Soo ; Moon, Sung-Gi ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1097~1103
DOI : 10.5352/JLS.2006.16.7.1097
Community structure varies not only in space but also in time. We stand in one position which located at Sinhung-ri, Sacheon-ci and observe the flora of area as time passes. This area begins after disturbance on sites where organisms are already present like secondary succession. In 2001, there are invaded by Trapa japonica, Salvinia natans, and Potamogon cristatus. Four or five years later, this abandoned areas support of clover, pine, and oak, resulting in a shifting pattern of species dominance and diversity through time. Patterns of community structure such as species composition and ecological diversity have shown through seasons and five years, that is the topic of this study. The values of turnover (TO) were changes through time intervals at three regions. The gradual and seemingly directional change in the structure of the community through time from aquatic like to field. Thus, we can observe the process of secondary succession at Sinhung-ri in the very short time.
The Enzymatic Regulatory Effects of Laninaria japonica Fucoidan Extract in Hepatotoxicity
Kang, Kum-Suk ; Nam, Chun-Suk ; Park, Eun-Kyung ; Ha, Bae-Jin ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1104~1108
DOI : 10.5352/JLS.2006.16.7.1104
The purpose of this study was to investigated the effects of Laminaria japonica fucoidan extract (LJFE) through the enzymatic regulation against the hepatotoxicity-inducing carbon tetrachloride
in LJFE and
rats. LJFE of 100mg/kg concentration was intraperitoneally administered into rats at dose of 1.5m11kg for 14 days. On the day 15, 3.3ml/kg of
dissolved in olive oil (1:1) was injected 12 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) in serum of rats, superoxide dismutase(SOD) in mitochondrial fraction, and catalase(CAT), glutathione peroxidase(GPx) in liver homogenate.
markedly increased the levels of GOT and GPT, and significantly decreased those of SOD, CAT and GPx. But LIFE pretreatment decreased the levels of GOT and GPT, by 40% and 64%, respectively and increased those of SOD, CAT and GPx, by 114%, 36.1% and 55.9%, respectively These results showed the LIFE had the enzymatic regulatory effects against the hepatotoxicity-inducing
in the preventive way.
Detection of Unusual Allele in D System of Red Cell Alloantigens Found in the Korean Native Horse
Cho, Gil-Jae ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1109~1111
DOI : 10.5352/JLS.2006.16.7.1109
The present study was carried out to investigate the blood markers of the Korean native horse. A total number of 158 horses were tested using microhaemagglutination with 11D system reagents (Da, Db, Dc, Dd, De, Df, Dg, Dh, Dk, Dm and Dn). Of the 158 horses, 3 horses showed an unusual D system phenogroups; these phenogroups may be silent (null) alleles, De or Dk and Dc, respectively. Dacdfgm, Dacdfgmn, and Ddegmn phenogroups were recognized. These results present basic information for detecting the genetic markers among the Korean native and alien horses.
Studies of an alcA Gene Involved in Alcaligin Siderophore Biosynthesis in Bordetella bronchiseptica
Hwang, Ho-Soon ; Kim, Young-Hee ; Kim, Sam-Woong ; Yu, Jong-Earn ; Yoo, Ah-Young ; Kang, Ho-Young ; Lee, Tae-Ho ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1112~1118
DOI : 10.5352/JLS.2006.16.7.1112
Bordetella bronchiseptica, the agent of swine atrophic rhinitis and kennel cough in dogs, is a mucosal pathogen and produces the hydroxamate type alcaligin siderophore under iron-limited conditions. Genes involved in alcaligin siderophore biosynthesis are contained in an alcABCDE operon. In order to provide direct evidence for the role of AlcA in alcaligin biosynthesis, we needed a B. bronchiseptica mutant carrying alcA gene deletion. A 0.6 kb alcA 5'-flanking and 0.7kb 3'-flanking DNA fragments were PCR amplified with the use of pCP1.11 as a template DNA. The 5'-and 3'-flanking DNA fragments were joined in a suicide plasmid, resulting in a recombinant suicide plasmid pDM1. After introduction of pDM1 into B. bronchiseptica by conjugation, the allelic exchange technique was performed and a B. bronchiseptica alcA deletion mutant, named B. bronchiseptica H1, was obtained. The mutant strain produced reduced amount of siderophore as expected. When a plasmid containing complete alcA gene was transformed back into the mutant, the complemented mutant recovered ability of siderophore production. These results indicated that AlcA is one of essential components for the alcaligin siderophore biosynthesis. The mutant strains obtained in this study will be used in the further studies for the biochemical function of AlcA.
Genetic Relationship Among Lycoris Species Using RAPD Analysis, the Native Distribution and Flowering Characteristics
Cha, Jae-Young ; Jung, Yen-Ok ; Shin, Sang-Min ; Kang, Yun-Kyung ; Park, Jong-Kun ; Park, Nou-Bog ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1119~1122
DOI : 10.5352/JLS.2006.16.7.1119
Ten species of Lycoris was selected for establishment of phylogenetic relationships using random amplified polymorphic DNA (RAPD) analysis, the native distribution and flowering characterestics. On the basis of the dendrogram constructed with the similarity coefficients, 10 Lycoris species were divided into two clusters. L. sprengeri and L. incannta were showed very high similarity in the RAPD analysis, same flowering time and flower color as pink. The leaf of L. squamigera. L. sanguinea, L. koreana, L. sprengeri and L. incanata emergenced in spring. The L. squamigera and L. sanguinea were showed high similarity in same cluster. Also L. koreana, L. sprengeri and L. incanata were showed high similarity in same cluster. The flower of L. radiata, L. radiata var pumila, L. albiflora and L. traubii was spider. These species was showed very low similarity in another Lycoris species.
Analysis of Amino Acids, Vitamins and Minerals of Fruiting Body of Fomitopsis pinicola
Ding, J.L. ; Shin, H.J. ; Cha, W.S. ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1123~1126
DOI : 10.5352/JLS.2006.16.7.1123
The nutritional composition of Fomitopsis pinicola (F. pinicola) fruiting body has been analyzed for medicinal and edible uses. The contents of crude fibers, carbohydrates, crude protein, moisture, crude fats and ashes were 43.3%, 26.3%, 12.8%, 12.6%, 3.3% and 1.7%, respectively. Eighteen amino acids were found in F. pinicola. Among total amino acids, glutamate content was the highest (457 mg/100 g dry mushroom) and arginine, glycine, valine, aspartate and isoleucine were followed. Concerning free amino acids, glutamine, arginine, trytophan, and glutamate were dominant. The vitamin E content was the highest (276 mg/100 g dry mushroom), then vitamin H and vitamin B, were followed. The mineral contents were as follows: K 165.06 mg, P77.57 mg, Mg 46.11 mg, Fe 21.56 mg, and Ca 16.90 mg based on 100 g dry mushroom.
Effects of Pleurotus eryngii on Lipid Levels and Enzyme Activity in Male Rats Fed High Cholesterol Diets
Lee, Choong-Un ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1127~1132
DOI : 10.5352/JLS.2006.16.7.1127
Effects of dietary supplementation of Pleurotus eryngii(PE) to a diet containig the cholesterol on lipid levels in the liver and serum, and enzyme activities of rats were studied by feeding 8-weeks old male rats with the four diets for 4 weeks, respectively: normal diet, control diet (10% fat + 0.5% cholesterol), 3% and 5% PE diets (control diet + 3% and 5% PE dry powder). The body weight gains and food efficiency ratios of the 3% and 5% PE diet groups were significantly lower than those of the control group. The liver and epididymal fat pad weights, and hepatic triglyceride Levels of the 5% PE diet group were more significantly decreased than those of the control group. The concentrations in serum triglyceride, total cholesterol, LDL+VLDL-cholesterol, and atherogenic index ratios were significantly lower in the 3% or 5% PE groups compared to those of the control group. Ratio of HDL-cholesterol to total-cholesterol was significantly higher in the 3% and 5% PE groups compared to that of the control group. The fecal total lipid excretion of the 5% PE diet group was more significantly decreased than that of the control group. There was no significant difference in the hepatic cholesterol, and the serum HDL-cholesterol concentrations, and the GOT, GPT and
activities among the experimental groups. These results showed that the Pleurotus eryngii powder feeding decreased the total cholesterol, LDL-cholesterol and atherogenic index, and increased the ratio of HDL-cholesterol to total-cholesterol in serum of the rats.
Purification of Human HtrA1 Expressed in E. coli and Characterization of Its Serine Protease Activity
Kim, Kyung-Hee ; Kim, Sang-Soo ; Kim, Goo-Young ; Rhim, Hyang-Shuk ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1133~1140
DOI : 10.5352/JLS.2006.16.7.1133
Human HtrA1 (High temperature requirement protein A1) is a homologue of the E. coli periplasmic serine protease HtrA. A recent study has demonstrated that HtrA1 is a serine protease involved in processing of insulin like growth factor binding protein (ICFBP), indicating that it serves as an important regulator of IGF activity. Additionally, several lines of evidence suggest a striking correlation between proteolytic activity of HtrA1 serine protease and the pathogenesis of several diseases; however, physiological roles of HtrA1 remain to be elucidated. We used the pGEX bacterial expression system to develop a simple and rapid method for purifying HtrA1, and the recombinant HtrA1 protein was utilized to investigate the optimal conditions in executing its proteolytic activity. The proteolytically active HtrA1 was purified to approximately 85% purity, although the yield of the recombinant HtrA1 protein was slightly low
for 1 liter E. coli culture). Using in vitro endoproteolytic cleavage assay, we identified that the HtrA1 serine protease activity was dependent on the enzyme concentration and the incubation time and that the best reaction temperature was
. We arbitrary defined one unit of proteolytic activity of the HtrA1 serine protease as 200nM of HtrA1 that cleaves half of
during 3 hr incubation at
. Our study provides a method for generating useful reagents to investigate the molecular mechanisms by which HtrA1 serine protease activity contributes in regulating its physiological function and to identify natural substrates of HtrA1.
Cytohistological Study of Development of Callus and Adventitious Shoots from Cultured Stem of Vigna radiata
Park, Jong-Bum ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1141~1147
DOI : 10.5352/JLS.2006.16.7.1141
This study was carried out to establish a reproducible culture system for callus formation and adventitious shoot development from young stem segments of Vigna radinta, and histological work for orgin of callus tissue and adventitious shoot. Induction of callus from young stem explants of Vigna radiata was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. For the adventitious shoot regeneration from the callus tissues, the hormone combination of 0.75 mg/L NAA, 1.5 mg/L kinetin and MS salts resulted in about 21% efficiency. Histological examination showed that callus tissues originated from out-growths by callus cambium rings with do novo meristematic activities, which were localized at the outside of the vascular cambium. Adventitious shoots were developed from shoot apical meristem originated from the surface of callus masses. The shoot apical meristem produced leaf primordium, which then became leaf.
Cloning and Identification of Essential Residues for Thermostable β-glucosidase (BgIB) from Thermotoga maritima
Hong, Su-Young ; Cho, Kye-Man ; Kim, Yong-Hee ; Hong, Sun-Joo ; Cho, Soo-Jeong ; Cho, Yong-Un ; Kim, Hoon ; Yun, Han-Dae ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1148~1157
DOI : 10.5352/JLS.2006.16.7.1148
A hyperthermophilic bacterium Thernotoga maritima produced thermostable
. The gene encoding
from T. maritima MSB8 was cloned and expressed in Escherichia coli. The en-zyme (BgIB) hydrolyzed
linkages between glucose and alkyl, aryl of saccharide groups such as salicin, arbutin, and
. The insert DNA contained ORF with 2,166 bp encodes a 721 amino acids (calculated molecular mass of 80,964 and pl of 4.93). The amino a.id sequence of BglB showed the similarity to family 3 glycosyl hydrolases. The molecular weight of the enzyme was estimated to be approximately 81kDa by MUG-nondenaturing PAGE (4-methylumbelliferyl 13-D-glucoside-nondenaturing polyacrylamide gel electophoresis) and SDS-PACE. The
exhibited maximal activity at pH 7.0 and
. By exchanging two possible residues (Glu-232 and Asp-242) to Ala by site-directed mutagenesis method, it was found that these were essential for enzymatic activity.
Isolation of Acinetobacter calcoaceticus BP-2 Capable of Degradation of Bisphenol A
Kwon, Gi-Seok ; Kim, Dong-Geol ; Lee, Jung-Bok ; Shin, Kee-Sun ; Kum, Eun-Joo ; Sohn, Ho-Yong ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1158~1163
DOI : 10.5352/JLS.2006.16.7.1158
Bisphenol A (BPA), 2,2-bis(4-hydroxyphenyl) propane, has been widely used as a monomer for production of epoxy resins and polycarbonate plastics, and final products of BPA include adhesives, protective coatings, paints, optical lens, building materials, compact disks and other electrical parts. Since BPA is a toxic chemical to elicit acute cell cytotoxicity and chronic endocrine disrupting activity, the degradation of BPA has been focused during last decades. To overcome the problem of photo-, and chemical-degradation of BPA, in this study, a bacterium that is able to biodegrade BPA, was isolated. The bacterium, isolated froln the soil of plastic factory, was identified as Acinetobacter calcoaceticus (strain BP-2) based on physiological and 16S rDNA sequencing analysis. A. calcoaceticus BP-2 was able to grow in the presence of
BPA. Biodegradation experiments showed that BP-2 mineralized BPA via 4-hydroxybenzoic acid and 4-hydroxyacetophenone, and average degradation rate was
under optimal conditions (pH 7 and
). In high density resting cell
experiments, the maximal degradation rate was increased to
. Our results suggest that BP-2 has high potential as a catalyst for practical BPA bioremediation.
Effect of Feeding of Citrus Byproduct on the Physicochemical Properties and Palatability of Pork Loin during Growing Period
Jung, In-Chul ; Moon, Yoon-Hee ; Yang, Sung-Joo ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1164~1168
DOI : 10.5352/JLS.2006.16.7.1164
This study was carried out to investigate the possible for utilization of garbage in pig feed by investigating the functional characteristics of pork from pigs fed dried citrus byproduct. The samples consisted of the pork loin from pigs not fed with dried citrus pulp (DCP-0) and fed with 6% and 10% dried citrus pulp during growing and fattening period (DCP-1) there is no respective comparison here The pH, VBN content, TBARS value, bacterial counts, surface color, water holding capacity, loss degree and rheological properties of the samples were determined by physicochemical properties, and the sensory scores were evaluated. The pH, VBN content, surface color, water holding capacity and loss degree were not different between the samples, but the TBARS value and bacterial counts of DCP-1 were significantly lower than those of the DCP-0 (p<0.05). The cohesiveness, gumminess, chewiness, shear forte value, taste, flavor, tenderness, juiciness and palatability were not different between the samples, but the hardness of DCP-0 was higher than that of the DCP-1 and the springiness of DCP-1 was higher than that of the DCP-0 (p<0.05).
Effects of Ulva lactuca Extracts on Cytotoxicity of Cancer Cell Lines and Immune Stimulation
Jang, Min-Kyung ; Kim, Nam-Young ; Lee, Dong-Geun ; Lee, Jae-Hwa ; Ha, Jong-Myung ; Ha, Bae-Jin ; Kim, Mi-Hyang ; Bae, Song-Ja ; Jang, Jeong-Su ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1169~1173
DOI : 10.5352/JLS.2006.16.7.1169
Extracted fractions of the green seaweed Ulva lactuca were studied to verify the cytotoxicity and immunostimulating activity. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with solvents such as hexane, ethyl ether, methanol, butanol and
. The cytotoxic effects of U. lactuca fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2) and rat glioma cell line (C6) were investigated. Ethyl ether fraction showed the highest cytotoxicity against all four cell lines tested. In addition,
fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of U. lactuca fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of U. lactuca extract stimulated NO production with concentration dependant manner. These results suggest that U. lactuca may be a useful candidate for a natural cancer preventing and immune-stimulating agents.
Effect of a Combinacon of Mental Activity with Experimental Subway Noise on Hematology
Choi, Seok-Cheol ; Hyun, Kyung-Yae ; Park, Jae-Hyun ; Kwon, Heun-Young ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1174~1180
DOI : 10.5352/JLS.2006.16.7.1174
The present study was sought to clarify whether the combination of mental activity with subway noise affects hematological variables. Fifty-six healthy volunteers participated in this experiment and underwent a stress task consisting of combination_of mental activity (mental arithmetic) with subway noise for 50 min and 60min of recovery after the end of the stress task. Venous blood samples were collected for measuring CBC, prothrombin time (PT), activated partial thromboplastin time (aPTT), erythrocyte sedimentation rate (ESR), fibrinogen concentration, D-dimer and high sensitive C-reactive protein (H-CRP) levels before (baseline), 50min of stress task (S-50m), and 60 min of recovery (R-60m). Total leukocyte, neutrophil and lymphocyte counts significantly increased at R-60m compared with baselines. RBC count at S-50m was higher, while monocyte counts at S-50m and R-60m were lower than those of baselines. aPTTs shortened at S-50m and R-60m, but PT reduced at R-60m as compared with baselines. D-dimer and H-CRP levels at S-50m and R-60m were significantly higher than those of baselines. These findings imply that a combination of mental activity with subway noise nay cause leukocytosis, homo-concentration, shortened PT and aPTT, decreased ESR, and raised D-dimer and H-CRP levels, suggesting possible development of inflammation and prothrombogenic reaction attributable to a subway environment.
Antiinflammatory Effects of New Chemical Compounds, HS-1580 Series (HS-1580, HS-1581, HS-1582)
Kim, Ji-Young ; Kim, Kyun-Ha ; Suh, Hong-Suk ; Choi, Won-Chul ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1181~1187
DOI : 10.5352/JLS.2006.16.7.1181
HS-1580 series (HS-1580, HS-1581, HS-1582) can produce anti-imflammatory effects were synthesized from the marine algae extraction in 2,3,6-tribromo-4,5-dihydroxy benzyl methyl ether (TDB). Raw 264.7 cells were pre-treated with
lipopolysaccharide (LPS) and later treated with HS-1580 series. These cells of inflammatory mediators were tested as well. Nitric oxide (NO) is related to autoimmune disease and is produced by inducible NOS (iNOS). When treated with HS-1580 series, the product of NO will reduce in a dose-dependent manner. HS-1580 series significantly inhibit the iNOS protein expression. Cyclooxygenase (COX) involves with the various physiologic events and catalyzes in prostaglandin. HS-1580 series also inhibit the COX-2 protein expression as well as pro-inflammatory cytokines production such as tumor necrosis
. These upcoming results suggest that HS-1580 series have anti-inflammatory efforts in Raw 264.7 cells by inhibiting such as iNOS, COX-2,
as inflammatory mediators.
Bioinformatics Analysis of Gene Expression Regulation by Transposable Elements in Dementia Patients
Kim, Dae-Soo ; Huh, Jae-Won ; Ha, Hong-Seok ; Kim, Tae-Hong ; Jo, Un-Jong ; Kim, Heui-Soo ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1188~1194
DOI : 10.5352/JLS.2006.16.7.1188
Dementia is a progressive disease of increasing the dysfunction of intellectual and physical ability. In the aging society, many families are suffering from the caring the patients who are diagnosed with dementia. However, dementia is a complex disease affected by the genetic and environmental agents. In the present study, we investigated the transposable elements in relation to dementia. From the analysis of dementia EST (expressed sequence tag) sequences, we found dementia candidate genes, and analyzed expression profiles and repeat elements using bioinformatics tools. This analysis showed that 98 genes were affected in their mRNA sequences by transposable elements expression. Their expressions were affected by the integration of different transposable elements (SINE, LINE, LTR, DNA) during the primate evolution. We believe that our work will be of significant interest to genome scientists, and may help them gain insight into implication of transposable elements expression in dementia.
Analyses of Morphological Characteristics, Antibiotic Effect, and Molecular Cytogenetics in Baegseon (Dictamnus dasycarpus Turcz)
Chung, Dae-Soo ; Cho, Chang-Woo ; Kim, Kyung-Mee ; Heo, Jee-Eun ; Kim, Soo-Young ; Chung, Young-Soo ; Kim, Doh-Hoon ; Bang, Jae-Wook ; Cui, Zheng ; Lee, Jai-Heon ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1195~1198
DOI : 10.5352/JLS.2006.16.7.1195
The importance of wild plant resources along with the development of high biotecyhnology is highlighted for exploitation of new materials which can make the added value. The goal of this study is providing fundamental data bases for developing new materials through the analyses of morphological characteristics, antibiotics and molecular cytogenetics in Baegseon (Dictamnus dasycarpus Turcz.). Baegseon has several characteristics; there are two types of flower color, pink and white, the seed germination starts about February 20th, the maximum flowering season is around May 17th in southern Korea, the growth period is about 60 days. The number of chromosomes are 2n=2x=36, the size of chromosomes in metaphase is
. The amount of 2C nuclear DNA is 1.93pg, and there is no variation of genome size amoung varieties. The extraction juice of baegseon young roots has the excellent antibiotic activity against the mold (Botrytis cinerea).
Inhibition of ERK1/2 Activation and Cytoskeleton Rearrangement by the Recombinant Protein of Plasminogen Kringle 5
Ha, Jung-Min ; Kim, Hyun-Kyung ; Kim, Myoung-Rae ; Joe, Young-Ae ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1199~1206
DOI : 10.5352/JLS.2006.16.7.1199
Plasminogen kringle 5 is a potent inhibitor of endothelial tell proliferation like an endogenous angiogenesis inhibitor, angiostatin consisting of plasminogen kringles 1-4. In this study, we produced the recombinant protein of plasminogen kringle 5 (PK5) employing an Pichia expression system and examined its. effect on~endothelial cell migration and its possible inhibitory mechanism. PK5 was expressed in Pichia pastoris GS115 by fusion of the cDNA spanning from Thr456 to Phe546 to the secretion signal sequence of a-factor prepro-peptide. After methanol induction, the secreted PK5 was purified by using S-spin column. SDS-PACE analysis of the purified protein showed one major band of approximately 10kDa. In in vitro migration assays, the purified protein inhibited dose-dependently the migration of human umbilical endothelial cells (HUVECs) induced by basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF) with an
of approximately 500nM. Accordingly, it inhibited bfGF-stimulated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in HUVECs at 500nM. In addition, it also potently inhibited bFGF-induced cytoskeletal rearrangement of HUVECs. Thus, these results suggest that Pichia-produced PK5 effectively inhibits endothelial cell migration, in part by suppression of ERK1/2 activation and blocking cytoskeleton rearrangement.
Tumor Necrosis factor-α Promotes Osteogenesis of Human Bone Marrow-derived Mesenchymal Stem Cells through JNK-dependent Pathway
Kim, Mi-Ra ; Song, Hae-Young ; Kim, Jae-Ho ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1207~1213
DOI : 10.5352/JLS.2006.16.7.1207
has been implicated in skeletal diseases by promoting bone loss in inflammatory bone diseases. In the present study, we examined the effects of
on osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs).
dose-dependently promoted matrix mineralization of hBMSCs with a maximal stimulation at 2ng/ml.
increased expression of alkaline phosphatase, which plays a crucial role for the matrix deposition. The
osteoblastic differentiation was not affected by
inhibitors, BAY and SN50. However, a JNK-specific inhibitor, SP600125 completely abolished the
matrix mineralization and expression of alkaline phosphatase. These results suggest that
enhances osteoblastic differentiation of hBMSCs through JNK-dependent pathway.
Sedative Effects of Combined Administration of 4-Hydroxy-3-methoxybenzaldehyde, a Component of Gastrodia elata, and 2,3-Dihydroxybenzaldehyde in Rats
Lee, Dong-Ung ; Choi, Hyung-Chul ; Lee, Kwang-Youn ; Lee, Soo-Kwan ; Kim, Jung-Ae ; Yong, Chul-Soon ; Kim, Jin-Sook ; Huh, Geun ; Shin, Son-Moon ; Koo, Byung-Soo ; Ha, Jeoung-Hee ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1214~1218
DOI : 10.5352/JLS.2006.16.7.1214
The present study was performed to investigate the sedative effects of the combined administration of phenolic compounds. 4-hydroxy-3-methoxybenzaldehyde, a component of Gastrodia elnta, showing positive GABAergic neuromodulation was administered intraperitoneally together with an identical dose of 2,3-dihydroxybenzaldehyde, a potent antioxidant, to the rats and then evaluated for its effects on the convulsion, the hypnosis, the inxiety and the muscle relaxation. Combined administration of both compounds significantly reduced the pentyleneterazole-induced lethality. In addition, this mixture significantly enhanced the pentobarbital-induced sleeping time. Contrary to the anticonvulsive and sedative effects, the combined administration did not exhibit anxiolytic or muscle relaxant activities. These results indicated that the combined treatment of 2,3-dihydroxybenzaldehtyde and 4-hydroxy-3-methoxybenzaldehyde with different effects leads to the anticonvulsion and/or sedation
Effect of Capsosiphon fulvecense Extract on Collagen Content of Connective Tissues in Ovariectomized Rats
Park, Mi-Hwa ; Kim, Mi-Hyang ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1219~1224
DOI : 10.5352/JLS.2006.16.7.1219
Osteoporosis is one of the major health problem affecting postmenopausal women. Estrogen deficiency results in an increase in bone turnover, lead to bone resorption and an increase risk of fracture. The aim of this study was to evaluate the effects of Capsosiphon fulvecense extract (SCF) on the collagen content of the connective tissues and alkaline phosphatase activity of serum in ovariectomized estrogen-deficient rats. Three groups were surgically ovariectomized (OVX). The fourth group was sham operated. Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), ovariectomized rats supplemented with CsF at 50mg/kg body wt (OVX-CSF50) and ovariectomized rats supplemented with CsF at 200mg/kg body wt (OVX-CSF200). The Capsosiphon fulvecense extracts were orally administrated at 1mL per day. The ovariectomy caused a decreasing in the levels of collagen content in bone, cartilage, skin and lung tissues. However CSF groups, supplementation with Capsosiphon fulvecense extract, were increased the level of collagen content in bone, cartilage, skin and lung tissues than OVX-control group. Alkaline phosphatase activity also were increased and calcium levels were decreased than OVX-control on serum. These results suggest that Capsosiphon fulvecense supplementation prevents postmenopausal bone loss, thus it may be used possibly to improve the quality of life in menopausal women.
Mutant cAMP Receptor Protein Binds to DNA without DNA Bending
Gang, Jong-Back ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1225~1228
DOI : 10.5352/JLS.2006.16.7.1225
Cyclic AMP receptor protein (CRP) complexed with cAMP binds to DNA and induces sharp DNA bending around
degree. Previous publication (5), however, reported that mutant CRP:cGMP complex showed high migration rate relative to mutant CRP:cAMP complex on native polyacrylamide gel. To confirm DNA structural change in the presence of CRP and cyclic nucleotide, molar cyclization factor
 was measured with 6 constructed DNA fragments. Nonlinear regression analysis of
data indicated that mutant CRP did not induce DNA bending in the presence of cGMP but bent DNA in the presence of cAMP without any helical twist change in DNA.
Up-regulation of Matrix Metalloproteinase-9 in Smooth Muscle Cell Undergoing Death
Lee, Kyeong-Ah ; Kim, Sun-Mi ; Kim, Koan-Hoi ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1229~1234
DOI : 10.5352/JLS.2006.16.7.1229
This study investigated whether matrix metalloproteinases (MMPs) can be modified in apoptotic smooth muscle cell (SMC) using the SMC that undergoes apoptotic death by expressing Fas-associated death domain containing protein (FADD) when they are grown without tetracycline in culture medium. In the absence of tetracycline, FADD-SMC lost adherence and showed the fragmentation of the nuclei. In proportion to duration of tetracycline removal, phosphorylated form of p38 MAPK and of ERK increased, whereas phosphorylation of protein kinase B (PKB) was not changed very much in response to tetracycline The levels of cyclin A and cyclin D were also decreased in a time dependent manner. Up-regulation of MMP-9 expression and activity was observed when the SMC were grown without tetracycline. Immunoreactivity of MMP-9 was detected from both attached and floating FADD-SMCs grown without tetracycline. An inhibitor of MAPK kinase, PD098059, and an inhibitor of p38 MAPK, SB203580, inhibited the up-regulation of MMP-9. Treatment of the SMC with a synthetic MMP inhibitor, BB94, attenuated death occurring in the absence of tetracycline. These results indicate that SMC undergoing death is able to up-regulate MMP-9 and that the enzyme can affect cell viability.
Modulation of Cell Cycle Regulators by Sulforaphane in Human Mepatocarcinoma HepG2 Cells
Bae, Song-Ja ; Kim, Gi-Young ; Yoo, Young-Hyun ; Choi, Byung-Tae ; Choi, Yung-Hyun ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1235~1242
DOI : 10.5352/JLS.2006.16.7.1235
Sulforaphane, an isothiocyanate derived from hydrolysis of glucoraphanin in broccoli and other cruciferous vegetables, was shown to induce phase II detoxification enzymes and inhibit chemically induced mammary tumors in rodents. Recently, sulforaphane is known to induce cell cycle arrest and apoptosis in human canter cells, however its molecular mechanisms are poorly understood. In tile present study, we demonstrated that sulforaphane acted to inhibit proliferation and induce morphological changes of human hepatocarcinoma HepG2 cells. Treatment of HepG2 cells with
sulforaphane resulted in significant G2/M cell cycle arrest as determined by DNA flow cytometry. Moreover,
sulforaphane significantly induced the population of sub-G1 cells suggesting that sulforaphane induced apoptosis. This anti-proliferative effect of sulforaphane was accompanied by a marked inhibition of ryclin A, cyclin 31 and Cdc2 protein. However, the levels of tumor suppressor p53 and Cdk inhibitor p21 mRNA and protein expression were significantly increased by sulforaphane treatment in a concentration-dependent manner. Although further studies are needed, the present work suggests that sulforaphane may be a potential rhemoprevetiveichemotherapeucc agent for the treatment of human cancer cells.
Comparison of Volatile Flavor Compounds in Commercial Crab-like Flavorants by Analyzing Methods
Cha, Yong-Jun ; Cho, Woo-Jin ; Jeong, Eun-Jeong ;
Journal of Life Science, volume 16, issue 7, 2006, Pages 1243~1249
DOI : 10.5352/JLS.2006.16.7.1243
Volatile flavor compounds in commercial crab-like flavorants were compared by mean of solid phase microextraction(SPME) and liquid continuous extraction (LLCE)/GC/MSD methods. A total of 86 volatile flavor compounds were detected. Of these, 71 were positively identified consisting mainly of sulfur-containing compounds (13), aldehydes (3), ketones (2), esters (26), alcohols (5), aromatic compounds (3), terpenes (8), acids (2) and miscellaneous compounds (9). SPME method was more effective than LLCE method in detection of volatile components in commercial crab-like flavorants. Eight S-, N-containing compounds such as dimethyl sulfide, dimethyl disulfide, dipropyl disulfide, 3-(methylthio)propyl acetate, 3-(methylthio) propanal, 3-(methylthio) propanol, 2-methyl-3-(methylthio) pyrazine and 2-methyl-5-(methylthio) pyrazine, 8 esters such as styrallyl acetate, ethyl acetate, isoamyl acetate, benzyl acetate, ethyl pentanoate, butyl pentanoate, isoamyl pentanoate and furfuryl acetate were considered as major components in crab-like flavorants.