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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
Selecting the target year
Regulation of cell size and cell number by LANCEOLATA1 gene in Arabidopsis
Cho, Kiu-Hyung ; Jun, Sang-Eun ; Jeong, Soon-Jae ; Yi, Young-Byung ; Kim, Gyung-Tae ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 1~5
DOI : 10.5352/JLS.2007.17.1.001
The Precesses for leaf development in dicotyledonous plants are surprisingly complex, while the mechanism of controlling and coordinating them is poorly understood. To characterize the fundamental features of the leaf development of Arabidopsis, we first attempted to isolate mutants that alter leaf morphology. Here, leaf morphological mutant of Arabidopsis, lanceolatal (lan1) which has small and narrow leaves have isolated and characterized. To clarify the function of LAN1 in organ development, we characterized lan1-7 mutant using an anatomical and genetic approach. The lan1-7 mutant had reduced size of foliage leaves and reduced dimensions of stems. A reduction both in cell size and in cell number was evident at the cellular level in the lan1 mutant, revealing that LAN1 gene appears to affect cell division at an earlier stage and cell elongation throughout the development of leaf primordia. from the analysis of heterogeneous plant with lan1 mutation and 35S-AG transgenic plant, AG gene is revealed to regulate leaf morphology under the control of 35S promoter. Thus, MADS-box gene was revealed to have some relationship to that of LAN1 gene at certain stage in leaf development processes.
Identification of catalytic acidic residues of levan fructotransferase from Microbacterium sp. AL-210
Sung, Hee-Kyung ; Moon, Keum-Ok ; Choi, Ki-Won ; Choi, Kyung-Hwa ; Hwang, Kyung-Ju ; Kim, Myo-Jung ; Cha, Jae-Ho ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 6~11
DOI : 10.5352/JLS.2007.17.1.006
]-Fructofuranosidases, a family 32 of glycoside hydrolases (GH32), share three conserved domains including the W(L/M)(C/N)DP(Q/N), FRDPK, and ECP(D/G) motifs. The functional role of the conserved acidic residues within three domains of levan fructotransferase, one of the
, from Microbacterium sp. AL-210 was studied by site-directed mutagenesis. Each mutant was overexpressed in E. coli BL21(DE3) and purified by using Hi-Trap chelating affinity chromatography and fast performance liquid chromatography. Substitution of Asp-63 by Ala, Asp-195 by Asn, and Glu-245 by Ala and Asp decreased the enzyme activity by approximately 100-fold compared to the wild-type enzyme. This result indicates that three acidic residues Asp-63, Asp-195, and Glu-245 play a major role in catalysis. Since the three acidic residues are present in a conserved position in inulinase, levanase, levanfructotransferase, and invertase, they are likely to have a common functional role as nucleophile, transition state stabilizer, and general acid in
Pretreatment of curcumin protects hippocampal neurons against excitotoxin-induced cell death
Kim, So-Jung ; Kim, Keun-Ho ; Kong, Kyoung-Hye ; Lee, Jae-Won ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 12~17
DOI : 10.5352/JLS.2007.17.1.012
Curcumin is a natural phenolic yellow curry spice, derived from the tumeric, which has been used for the treatment of diseases associated with oxidative stress and inflammation. Curcumin is known to have both anti-oxidative and anti-inflammatory properties. These properties can be beneficial to protect the brain from the neurodegenerative diseases. We now report the neuroprotective effects of curcumin pretreatment in primary hippocampal neurons to glutamate-induced excitotoxicity. Pretreatment of embryonic mouse hippocampal cell cultures with low does of curcumin protected neurons against glutamate-induced death, however, this neuroprotection was not correlated with the modulation of oxidative stress. Interestingly, high dose of curcumin showed the cytotoxicity in primary cultured hippocampal neurons. Immunoblot analyses showed that levels of stress response. protein HSP70 were significantly elevated in neurons exposed to low dose of curcumin, whereas levels of cleaved PARP were increased in neurons exposed to high dose of curcumin. These findings show that curcumin can modulate neuronal responses to glutamate, and suggest possible use of curcumin and related compounds in the prevention and/or treatment of neurodegenerative disorders.
High-level production and initial crystallization of a Fe65 PTB domain
Ro, Seung-Hyun ; Ha, Nam-Chul ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 18~23
DOI : 10.5352/JLS.2007.17.1.018
Fe65, a neuron-specific adaptor protein, has two phosphotyrosine binding (PTB) domains. The second PTB (PTB2) domain interacts with intracellular domain fragment (AICD) of amyloid beta precursor protein (APP). Recent studies suggested that tile complex is composed of AICD and Fe65 transactivates genes that are responsible for neuronal cell death in Alzheimer`s disease (AD). Therefore, a compound inhibiting the interaction between Fe65 and AICD can be a drug candidate to treat AD. However, it remains unclear how Fe65 recognizes AICD at a molecular level. Here, we report high-level production of the PTB2 domain of Fe65 in the baculovirus system. We found that the baculovirus system is an efficient method to obtain the Fe65 PTB2 domain, compared with the bacterial and mammalian expression systems. The purified recombinant protein was used for crystallization to determine its crystal structure helping to understand the molecular mechanism of Fe65-dependent signaling and to design its inhibitors.
Genetic Relationships between Gardenia jasminoides var. radicans and G. jasminoides for. grandiflora Using ISSR Markers
Huh, Man-Kyu ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 24~30
DOI : 10.5352/JLS.2007.17.1.024
Inter simple sequence repeat (ISSR) markers were performed in order to analyse the genetic relation-ships of both taxa of Gardenia jasminoides var. radicans and G. jasminoides for. grandifora. Over the 88 fragments, only one locus (ISSR-11-05) was specific to G. jasminoides var. radicans and only one (ISSR-09-05) G. jasminoides for. grandiflora. Although G. jasminoides var. radicans showed low levels of alleles and Shannon`s information index than G. jasminoides for. Grandiflora, however, there was not significant differences (p > 0.05). For both taxa the mean genetic diversity of natural populations was higher than that of cultivation populations. It was suggested that domestication processes via artificial selection do not have eroded the high levels of genetic diversity. ISSR markers were more effective in classifying natural populations of wild G. jasminoides in East Asia as well as cultivated G. jasminoides. The information about the phylogenetic relationship of G. jasminoides var. radicans and its closely related species is very valuable of the systematics of genus Gardenia, the origin of cultivated G. jasminoides, and future G. jasminoides breeding.
Lipid A of Salmonella typhimurium Suppressed T-cell Mitogen-Induced Proliferation of Murine spleen Cells in the Presence of Macrophage
Kang, Gyong-Suk ; Chung, Kyung-Tae ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 31~38
DOI : 10.5352/JLS.2007.17.1.031
Infection with virulent or attenuated Salmonella typhimuriumhas known to induce reduction in proliferative responses of spleen cells. We investigated a role of lipid A from S. typhimurium, a B cell mitogen, on proliferation of spleen cells by T cell mitogens such as concanavaline A and phytohemagglutinin under in vitro and ex vivo conditions. Lipid A alone induced proliferation of spleen cells in vitroin a dose-dependent manner. However, subsequent treatment of concanavaline A or phytohemagglutin in after lipid A treatment induced proliferation suppression of murine spleen cells in vitro and ex vivo. Removal of macrophages from spleen cells, which were obtained from a lipid A-injected mouse, restored proliferation by concanavaline A and phytohemagglutinin, indicating that macrophages appeared to play a role in lipid A-induced suppression. Secreted molecules from macrophages did not accounted for the suppression because suppressive effect was not achieved when the supernatant from macrophage-containing spleen cell culture was conditoned to macrophage-depleted spleen cell culture. Co-culture of spleen cells from lipid A-treated and - untreated mice showed proliferation suppression as increasing cell numbers of lipid A-treated mouse. These data suggested that the cell-to-cell contact of macrophage with splenic lymphocyte cells is responsible for immune responses against lipid A, which is applicable to the case of human S. typhi infection.
Isolation and Characterization of DNA Damaging Agent Sensitivity of rqh1 mutant from Schizosaccharomyce pombe
Lee, In-Hye ; Choi, In-Soon ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 39~44
DOI : 10.5352/JLS.2007.17.1.039
The Rqh1 gene is essential for vegetative growth in fission Yeast. The rqh1 mutant showed that sensitivity of DNA damaging agent, a wild range of phenotype including abnormal gene expression and cell elongation. This result showed that the rqhl-overexpression cell was sensitivity to DNA damaging agent like rqhl mutant. When Rqh1 have an over-expression by
promoter of pREP vector, rqh1 mutant DNA damaging agent sensitivity could be compensated. We isolated two strong mutant containing complementation gene, rqh156 and rqh172, respectively. This result observed that the DNA damaging agent sensitivity of rqhl mutant was complemented by the expression of rqh156 and rqh172. They induced mRNA expression in a dose-dependent manner HU, MMS and UV. The HU sensitivity of the rqhl was complemented by the expression of rqh156 and rqh172. The mRNA expression of rqh156 decreased on HU dose dependent but the mRNA expression of rqh172 did not decrease on HU dose dependent. The MMS and W sensitivity of the rqhl was complemented by the expression of rqh156 and rqh172. These results indicate that the isolated rqhl gene may play an important role in DNA metabolism.
Biological Activity of Tumor Necrosis Factor-α Secreted from Smooth Muscle Cell Overexpressing FADD
Kim, Sun-Mi ; Lee, Kyeong-Ah ; Kim, Koan-Hoi ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 45~50
DOI : 10.5352/JLS.2007.17.1.045
This study investigated biological activity of tumor necrosis factor
secreted from smooth muscle cell (SMC) destined for death by expressing Fas associated death domain containing protein (FADD) (FADD-SMC) when the cells are grown without tetracycline in culture medium. In the absence of tetracycline the FADD-SMC secreted approximately 1000 pg/ml
, whereas hardly detectable amount of the cytokine existed in the presence of tetracycline. The culture medium collected from the FADD-SMC grown in the absence of tetracycline increased phosphorylated form of p38 MAPK and up-regulated nuclear factor kappa B (NF-kB). The medium collected without tetracycline also caused death of L929 cells. Depletion of
with the soluble TNF receptor (sTNFR) inhibited the phosphorylation of p38 MAPK, the up-regulation of NF-kB activity and the death activity of the medium collected from FADD-SMC in the absence of tetracycline. These results indicate that
secreted from SMC undergoing death is biologically active and can affect cellular function.
Antioxidant Activities of Ulva lactuca Extracts with Different Solvents
Li, Hua-Yue ; Lee, Sung-Mok ; Lee, Dong-Geun ; Lee, Jae-Hwa ; Lee, Sang-Hyeon ; Ha, Bae-Jin ; Jang, Jeong-Su ; Kim, Won-Suk ; Ha, Jong-Myung ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 51~55
DOI : 10.5352/JLS.2007.17.1.051
The fractions of Ulva lactuca were studied to verify the antioxidant activities. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with the solvents such as hexane, ethyl ether, ethyl acetate, butanol and
. Furthermore, ethyl ether, ethyl acetate and aqueous fractions of U. lactuca were purified using HPLC. The antioxidant activities of purified samples from ethyl ether, ethyl acetate, and aqueous fractions were investigated using 1,1-diphenyl-2-picryl-hydrazil (DPPH). L-ascorbic acid, a positive control showed the highest DPPH radical scavenging activity. In addition, purified sample from aqueous fraction also showed relatively high activity. Purified sample from ethyl acetate fraction showed moderate activity, but purified sample from ethyl ether fraction showed the lowest activity. Dose dependent patterns were observed on all three samples tested. The lipid peroxidation inhibition activities of these three purified samples were also investigated. Purified sample from ethyl ether fraction of U. lactuca showed the highest activity and as strong activity as that of
, a positive control. These results suggest that U. lactuca may be a useful candidate for a natural antioxidant agent.
Molecular Events of Insulin Action Occur at Lipid Raft/Caveolae in Adipocytes
Bae, Sun-Sik ; Yun, Sung-Ji ; Kim, Eun-Kyung ; Kim, Chi-Dae ; Choi, Jang-Hyun ; Suh, Pann-Ghill ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 56~63
DOI : 10.5352/JLS.2007.17.1.056
Insulin stimulates the fusion of intracellular vesicles containing glucose transporter 4 (GLUT4) with plasma membrane in adipocytes and muscle cells. Here we show that adipocyte differentiation results in enhanced insulin sensitivity of glucose uptake. On the other hand, glucose uptake in response to platelet-derived growth factor (PDGF) stimulation was markedly reduced by adipocyte differentiation. Expression level of insulin receptor and caveolin-1 was dramatically increased during adipocyte differentiation. Adipocyte differentiation caused :ilightly enhanced activation of acutely transforming retrovirus AKT8 in rodent T cell lymphoma (Akt) by insulin stimulation. However, activation of Akt by PDGF stimulation was largely reduced. Activation of ERK was not detected in both fibroblasts and adipocytes after stimulation with insulin. PDGF-dependent activation of ERK was reduced by adipocyte differentiation. Insulin-dependent glucose uptake was abrogated by LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, in both fibroblasts and adipocytes. Also disassembly of caveolae structure by
caused impairment of Akt activation and glucose uptake. Finally, insulin receptor, Akt, SH2-domain-containing inositol 5-phosphatase 2 (SHIP2), and regulatory subunit of PI3K are localized at lipid raft domain and the translocation was facilitated upon insulin stimulation. Given these results, we suggest that lipid raft provide proper site for insulin action for glucose uptake.
Effect of fisetin on UVB-induced apoptosis and DNA single strand breaks in NIH3T3 cells
Jeong, Se-Jin ; Kim, Don-Young ; Han, Seol-Hee ; Shin, Sang-Min ; Cha, Jae-Young ; Park, Nou-Bog ; Lee, Jung-Sup ; Park, Jong-Kun ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 64~69
DOI : 10.5352/JLS.2007.17.1.064
In the present study, we have investigated the effect of fisetin on the apoptosis and DNA single strand breaks in ultraviolet light B (UVB)-exposed NIH3T3 cells. Exposure of cells to UVB light
and post-incubation in growth medium for 48 hr resulted in about 50% of cells with apoptotic nuclear fragmentation. Addition of various concentrations of fisetin in the postincubation medium, however, significantly reduced the apoptotic nuclear fragmentation as compared with the values expected when the effects are additive and independent. DNA single strand breaks induced by UVB exposure were also significantly decreased by postincubation with fisetin. By Western blot analysis, fisetin post-incubation was shown to attenuate the p53 upregulation upon UVB exposure. Furthermore, the decrease of proliferating cell nuclear antigen (PCNA) level upon UVB exposure was alleviated by fisetin postincubation. These results suggest that fisetin decrease the apoptosis and increae DNA repair in a possible association with alteration of p53 and PCNA levels in UVB-exposed cells.
Isolation and characterization of a marine bacterium Thalassomonas sp. SL-5 producing β-agarase
Lee, Dong-Geun ; Kim, Nam-Young ; Jang, Min-Kyung ; Lee, Ok-Hee ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 70~75
DOI : 10.5352/JLS.2007.17.1.070
A novel agar-degrading bacterium SL-5 was isolated from seashore of Homigot at Kyung-Buk province, and cultured in marine broth 2216 media. The bacterium SL-5 was identified as Thalassomonas genus by 16S rDNA sequencing with 96% identity. Growth rate was faster at
and agarase was produced as growth-related. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was
. Although the enzyme had no thermostability, the enzyme activity was remained over 80% at
. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is
. Thus, the enzyme would be useful for the industrial production of neoagarobiose.
Inhibitory Effect of Methanol Extracts and Solvent Fractions from Meju on Mutagenicity and Growth of Human Cancer Cells
Lim, Sun-Young ; Park, Kun-Young ; Lee, Sook-Hee ; Choi, Jae-Soo ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 76~81
DOI : 10.5352/JLS.2007.17.1.076
Inhibitory effects of methanol extracts and several solvent fractions from meju on mutagenicity in vitro genotoxicity (SOS chromotest) and growth of human cancer cells (AGS gastric adenocarcinoma and Hep 3B hepatocellular cancinoma cells) were studied. The treatment of meju methanol extracts
to SOS chromotest system inhibited N-methyl-N`-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 36%. However, the ethylacetate and dichloromethane fractions from meju methanol extracts showed the stronger antimutagenic effects (91% and 91%, respectively) in SOS chromotest. In sulforhodamine B (SRB) assay, the treatments of ethylacetate and dichloromethane fractions (2 mg/assay) significantly inhibited the growth of AGS and Hep 3B cancer cells by 64% and 71%, respectively. These results indicated that meju had inhibitor)r effects on MNNG in SOS mutagenic system and growth of human cancer cells, suggesting that its antimutagenic effect may be relative to activity of doenjang.
Effects of Various Ventilation Systems on the Carbon Dioxide Concentration and Fruiting Body Formation of King Oyster Mushroom (Pleurotus eryngii) Grown in Culture Bottles
Lee, Hyun-Uk ; Ahn, Mi-Jeong ; Lee, Shin-Woo ; Lee, Cheol-Ho ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 82~90
DOI : 10.5352/JLS.2007.17.1.082
In an attempt to establish the appropriate ventilation device for the bottle culture of king oyster mushroom (Pleurotus eryngii), we investigated carbon dioxide concentration and fruiting body formation according to the various ventilation systems within the mushroom house. In addition to, the efficiency of air circulation and growth rate as well as the appearance of physiologically abnormal phenotypes during their growth stage were also evaluated. four different ventilation devices, parallel-pressure type, positive-pressure type, negative-pressure type, and positive- and negative-pressure type were applied in this study. The positive-and negative-pressure type showed the highest efficiency of air circulation as
concentration was 800 ppm and the level of air current was relatively low compared to the other types (the
concentration of parallel-pressure type was 1,400 ppm). Moreover, the stipe length, the cap diameter, yield, and general quality grown in positive- and negative type ventilation device were also better than in the other three devices though it took slightly longer period for harvesting (18.4 days) than the others (17.6, 17.9 and 18.3 days). The appearance of physiologically abnormal phenotypes such as fruiting body lump, soft rot, and brown rot were significantly decreased in positive-and negative type compared to other types, while the appearance rates were not much different for other symptoms of bacterial ooze, stipe limb and stipe bumpy. In summary, we propose that the optimal ventilation system for the bottle culture of king oyster mushroom is positive- and negative type, and this device is expected to increase the total quality as well as yield all year around.
Changes in Quality of Pork Patty Containing Red Wine During Cold Storage
Youn, Dong-Hwa ; Moon, Yoon-Hee ; Jung, In-Chul ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 91~96
DOI : 10.5352/JLS.2007.17.1.091
This study was carried out to investigate the effect of addition of red wine on the water holding capacity, surface color, pH, TBARS value, VBN content and total bacterial counts of pork patties during cold storage. Pork patties were prepared by four type such as pork patty without red wine (control), pork paoy containing red wine 1% (RW-1), pork patty containing red wine 3% (RW-3) and pork patty containing red wine 5% (RW-5). The water holding capacity was increased significantly during cold storage, but was not influenced by addition of red wine (p<0.05). The
(yellowness) value was low by addition of red wine, was decreased during cold storage (p<0.05). The pH of pork patties were tend to increased with increase in cold storage period, the pH of RW-3 and RW-5 were significantly lower compared to control and RW-1 (p<0.05). The TBARS value of pork patties were tend to increased with increase in cold storage period, by increasing the amount of addition of red wine, the TBARS value were tend to decreased (p<0.05). The VBN content and total bacterial counts of pork patties were tend to increased with increase in cold storage period, and by increasing the amount of addition of red wine, the VBN content and total bacterial counts were tend to decreased during cold storage.
The Tissues and Blood Components of Opsariichthys uncirostris amurensis in the Muddy Water Area
Shin, Myung-Ja ; Lee, Jong-Eun ; Seo, Eul-Won ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 97~104
DOI : 10.5352/JLS.2007.17.1.097
Present study aims to investigate the effect of muddy water on gill and kidney tissues and blood components of Opsariichthys uncirostris amurensis in Imha reservoir. As a result of observing the gill tissues in muddy water with light and scanning electron microscopes, the edema, the exfoliation of epithelial cells and fusion of the secondary lamellae were observed. The space between gill lamellae was irregular, and a lots of muddy debris around the secondary lamellae were observed. In case of kidney tissues, the size of glomerulus was smaller and thickness of basal membrane was broader and the empty space in Bowman`s capsule was wider than those of the control. The blood cells of O. uncirostris amurensis in muddy water were increased in number. Of the white blood cells, acidophilic granulocytes were highly distributed, and the shapes were irregular. Among the plasma components, the concentration of TP, ALB, A/G ratio, TG, CHOL, and AST were low, whereas that of the inorganic materials was high. Based on the above results, it is considered that muddy water possibly involves in the decrease of respiratory and excretory rates, giving rise to secondary lesion of tissues.
Antimicrobial and Antioxidant Activities and Inhibition of Nitric Oxide Synthesis of Oak Wood Vinegar
Jung, Il-Sun ; Kim, Yu-Jung ; Gal, Sang-Wan ; Choi, Young-Ju ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 105~109
DOI : 10.5352/JLS.2007.17.1.105
This study was carried out to investigate the biological effects oak wood vinegar. Antimicrobial activity was tested in five microbial species at the concentration of 5 to
of oak wood vinegar by paper disc method. Growth of P. oleovoranse, P. vulgaris, E. coli, S. aureus and Prevotella intermedia was inhibited at a dose of as low as
of oak wood vinegar. Antioxidant activities were measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activities were 90% and 65% at the concentration of
of oak wood vinegar, respectively. Stimulation of the macrophages RAW264.7 cells with lipopolysaccharide (LPS) resulted in increased production of nitric oxide (NO) in the medium. However, the oak wood vinegar showed marked inhibition of NO synthesis in a dose-dependent manner. This result suggest that oak wood vinegar plays significant role for activation of immune system in the pathogenesis of inflammatory diseases.
Involvement of Early Growth Response Gene 1 (EGR-1) in Growth Suppression of the Human Colonic Tumor Cells By Apigenin and Its Derivative Isovitexin
Moon, Yu-Seok ; Cui, Lei-Guang ; Yang, Hyun ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 110~115
DOI : 10.5352/JLS.2007.17.1.110
It has been previously described that transcription factor early growth response gene product 1 (EGR-1) functions as a tumor suppressor gene. This study was conducted to demonstrate that EGR-1 induction by phytochemical apigenin and its derivative isovitexin can mediate the growth suppression of the intestinal epithelial tumor cells. Apigenin and isovitexin induced EGR-1 gene expression both in the dose and time-dependent manners. Moreover the induction was relatively late around 9-12 hr after treatment of HCT-116 cells, while several anti-inflammatory agent such as NSAIDS and catechins elicit the ECR-1 gene expression at much earlier time about 1-3 hr after treatment. In terms of signal transduction, ERK1/2 was critical for apigenin-induced EGR-1 gene expression and its promoter activation. When EGR-1 gene expression was blocked with EGR-1 small interference RNA, the cytotoxicity of apigenin in the human epithelial cells was attenuated, suggesting the involvement of EGR-1 in the anti-tumoric activity of apigenin. To link the EGR-1 induction to EGR-1-regulated gene products in colon cancer, NSAID-Activated Gene 1 (NAG-1) was demonstrated to be elevated by apigenin and isovitexin at 24-48 hr after treatment. Taken together, apigenin-activated ERK1/2 mediated EGR-1 gene induction, which was associated with suppression of the cellular viability by apigenin compound.
Pericarp Thickness of Korean Local Waxy Corn Lines Collected from Busan and Gyungnam
Lee, In-Sup ; Kim, Soon-Im ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 116~119
DOI : 10.5352/JLS.2007.17.1.116
To get abundant germplasm for developing new waxy corn varieties, pericarp thickness was measured and other factors were evaluated with a total of 142 Korean local waxy corn lines collected from various parts of Busan City and Gyungnam Province. The average pericarp thickness of Korean local waxy corn lines collected was
and the rate of thin pericarp under
was 70.4%. The pericarp thickness compared by the regions collected showed non significant difference, but which compared by the ear row number per ear showed significant difference $(MS
Optimization of gibberellin production by Fusarium prolifertum KGL0401 and its involvement in waito-c rice growth
Rim, Soon-Ok ; Lee, Jin-Hyung ; Lee, In-Jung ; Rhee, In-Koo ; Kim, Jong-Guk ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 120~124
DOI : 10.5352/JLS.2007.17.1.120
Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited higher GA productivity than wild type Gibberella fujikuroi. The :tim of this work was to find out an optimal culture condition for GA production. Various carbon(fructose, glucose, lactose, maltose, sucrose) and nitrogen(
, urea, glycine,
) sources were used for this study. GAs activities were analysed by gas chromatography and mass spectrometry(GC-MS). The highest yield of
was found in the growth medium supplemented with sucrose as carbon source and
as nitrogen source. The optimum carbon-nitrogen concentration for
production was found to be 0.5 M:0.17 M. Supernatant was prepared from the culture fluid of F. proliferatum KGL0401 cultured for 7 days at 3 0`E and the 10 ul supernatant was treated with 2 leaf-rice seedling.
Studies on the Amino Acid and Fatty Acid Compositions in the Seed and Pulpy Substance of Feral Peach (Prunus persica Batsch var. davidiana Max.)
Kim, Han-Soo ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 125~131
DOI : 10.5352/JLS.2007.17.1.125
Amino acid and fatty acid compositions of the physiological activity substance in the seed and pulpy substance of feral peach (Prunus persica Batsch var. davidiana Max.) were analyzed for the use as an biohealth functional processed products. The proximate compositions in the vacuum freeze dried seed and pulpy substance of feral peach were carbohydrate 63.92% and 75.11%, crude protein 27.85% and 12.77%, moisture 3.61% and 4.69%, crude fat 1.21% and 4.80%, crude ash 3.41% and 2.63%, respectively. Total amino acid contents in the protein of feral peach seed were 3,444.35 mg%, and the major amino acids were aspartic acid(681.10 mg%), glutamic acid(495.48 mg%), alanine(283.66 mg%), serine(251.36 mg%), proline(229.80 mg%), lysine(192.31 mg%) and leucine(191.34 mg%), respectively. Total amino acid contents in the protein of feral peach pulpy substance were 1,064.02 mg%, and the major amino acids followed aspartic acid(250.15 mg%), glutamic acid(129. 63 mg%), lysine, proline, leucine, alanine and serine, in a decreasing order. The richest total amino acid content contained in feral peach seed and pulpy substance was aspartic acid, followed by glutamic acid. The amount of free amino acids of feral peach seed were 6,215.34 ms%, and the major free amino acids were glutamic acid(827.25 mg%), threonine, valine and
acid, respectively. Free amino acid contents of pulpy substance were 683.82 mg%, and the major free amino acids were glutamic acid(339.49 mg%), serine proline, alanine and
acid. Especially, in the case of glutamic acid, it was highest. The compositions of major total fatty acid in the lipid feral peach (Prunus persica Batsch var. davidiana Max.) seed and pulpy sabstance were linoleic acid(
, n-6) and linolenic acid(
, n-3), particularly.
Enhancement of Soluble Expression of Alginate Lyase By Molecular Chaperone in E. coli.
Shin, Eun-Jung ; Lee, Jae-Hyung ; Park, So-Lim ; Kim, Hyeung-Rak ; Nam, Soo-Wa ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 132~136
DOI : 10.5352/JLS.2007.17.1.132
When alginate lyase gene (aly) from Pseudoalteromonas elyakovii was expressed in E. coli, most of the gene product was produced as aggregated insoluble particles known as inclusion bodies. In order to produce a soluble and active form of alginate lyase, E. coli cells fore cotransformed with the plasmids designed to permit coexpression of aly together with molecular chaperones such as DnaK/DnaJ/GrpE or GroEL/ES chaperones. The results revealed that the coexpression of aly together with DnaK/DnaJ/GrpE chaperone had a marked effect on the production of this protein as a soluble and active form, presumably through facilitating correct folding of alginate lyase protein. The optimal concentration of L-arabinose for the induction of DnaK/DnaJ/GrpE chaperone was found to be 0.05 mg/ml. When DnaK/DnaJ/GrpE chaperone was coexpressed, about 34% in the total alginate lyase was produced in the soluble fraction. By addition of 10% cetylpyridinium chloride, a clear zone around the colony coexpressing aly and DnaK/DnaJ/GrpE chaperone was formed, indicating that the alginate in the medium was hydrolyzed by active alginate lyase enzyme.
Characteristics of Monascus Natural Pigments Produced by Monascus sp. MK2-2
Jeon, Chun-Pyo ; Kim, Chang-Suk ; Lee, Jung-Bok ; Shin, Ji-Won ; Choi, Sung-Yeon ; Choi, Chung-Sig ; Lee, Oh-Seuk ; Kwon, Gi-Seok ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 137~142
DOI : 10.5352/JLS.2007.17.1.137
For the production of natural pigments with microbe, the strains which produced monascus pigment were isolated, and then culture condition and extraction condition were investigated. These results are summarized as follows; The strain which ran produce monascus natural pigment was isolated from natural microbial sources and we made mutant of this strain with UV(
, 30 second) irradiation. The mutant was identified as Monascus sp. MK2-2. The optimal culture conditions were investigated optimal medium containing 0.3% rice powder, 0.2% yeast extract, 0.3%
in a rotary shaker (120 rpm) for 5 days (initial pH 5.0), while the pigment production was determined at 24 hr intervals. The effective carbon sources were wheat flour > rice powder > fructose, and effective nitrogen sources were sodium nitrate >
for production of the monascus natural pigment. The pigment capacity is good from 17 to 22 in C/N ratio. The production amount of monascus natural pigment was 0.38 g per 1 kg of rice. Also, extract of red yeast rice had anti-thrombosis activity like a degree of aspirin.
Inhibition effect of neuronal death by Scutellaria baicalensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells
Kim, Wan-Sik ; Jung, Seung-Hyun ; Shin, Gil-Jo ; Moon, Il-Soo ; Lee, Won-Chol ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 143~149
DOI : 10.5352/JLS.2007.17.1.143
Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of retinoic acid receptor a (RARa). Rat cerebrocortical cells were grown in neurobasal medium. On DIV12 cells were treated with SB
and given a hypoxic shock
on DIV14. In situ hybridization using cRNA probe revealed that RARa mRNA punctae are distributed, in addition to nucleus, throughout neuronal dendrites, where SB upregulated its density by 69.8% (p
Microarray Analysis of Alteration in Gene Expression by Acori graminei rhizoma (AGR) Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells
Park, Dong-Jun ; Jung, Seung-Hyun ; Moon, Il-Soo ; Lee, Won-Chol ; Shin, Gil-Jo ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 150~161
DOI : 10.5352/JLS.2007.17.1.150
Acori graminei Rhizomn (AGR) is a perennial herb which has been used clinically as a traditional oriental medicine against stroke, Alzheimer`s disease, and vascular dementia. We investigated the effect of AGR on the modulation of gene expression profile in a hypoxic model of cultured rat cortical cells. Rat cerebrocortical cells were grown in Neurobasal medium. On DIV12, cells were treated with AGR
, given a hypoxic shock (2%
, 3 hr) on DIV14, and total RNAs were prepared one day after shock. Microarray analyses indicated that the expression levels of most genes were altered within the global M values +0.5 and -0.5, i.e., 40% increase or decrease. There were 750 genes which were upregulated by < global M +0,2, while 700 genes were downregulated by > global M -0.2. The overall profile of gene expression suggests that AGR suppresses apoptosis (upregulation of anti-apopotic genes such as TEGT, TIEG, Dad, p53, and downregulation of pro-apopotic genes such as DAPK, caspase 2, pdcd8), ROS (upregulation of RARa, AhR), and that AGR has neurotrophic effects (upregulation of Aktl, Akt2). These results provide a platform for investigation of the molecular mechanism of the effect of AGR in neuroprotection.
PCR Specific Primer for the Detection of Vibrio tapetis
Kim, Yeong-Jin ; Lee, Sun-Yi ; Cho, Hyo-Jin ; You, Sun-Nyung ; Kim, Cheol-Min ; Choi, Yong-Lark ; Park, Byoung-Keun ; Ahn, Soon-Cheol ;
Journal of Life Science, volume 17, issue 1, 2007, Pages 162~165
DOI : 10.5352/JLS.2007.17.1.162
Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of france, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.