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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
Selecting the target year
Investigation of Diseases of Thai koi, Anabas testudineus (BLOCH) from Farming Conditions in Winter
Ahmed, Gias Uddin ; Dhar, Mili ; Absar Khan, Mohammed Nurul ; Choi, Jae-Suk ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1309~1314
DOI : 10.5352/JLS.2007.17.10.1309
Investigation on health conditions of Thai koi (Anabas testudineus) were carried out through clinical and histopathological observations from different farms of Mymensingh district for seven months during August 2006 to February 2007. Fish sampling and water quality parameters (temperature, dissolved oxygen and pH) were monitored on a monthly basis. Clinical examination of fishes was also carried out for any kind of abnormalities at monthly intervals. Samples of skin, muscle, gill, liver and kidney were observed by histological technique. Among the water quality parameters the values of water temperature, dissolved oxygen were found to be at unfavorable level for fish during the colder months in the farms. Clinically it was observed that fishes were more affected from December to January and almost normal in appearance during August to September and February. Different clinical symptoms like scale loss, dermal lesion, ulcer and loss of caudal fin were noticed in December and January. In histopathological study, structures of fish organs were normal from August to September. In the months of October and November, minor pathologies were found to be started. Marked pathological changes like necrosis, pyknosis, inflammation, hemorrhage, hypertrophy, hyperplasia, missing of primary and secondary gill lamellae were observed in the months of December and January. Whereas, in the month of February the pathological condition of fish gradually reduced. Again when considered individual fish farm, fishes of Reliance Aqua Farm were more affected than Sotota Matshya Hatchery. The study showed that severity of clinical and pathological changes were increased in December and January. During the period EUS and protozoan diseases were noticed in A. testudineus of the investigated farms.
Radiographic Evaluation of Limb Bone Development in Miniature Porcine
Chang, Jin-Hwa ; Jung, Joo-Hyun ; Choi, Min-Cheol ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1315~1320
DOI : 10.5352/JLS.2007.17.10.1315
Epiphyseal development in the long bones was studied radiographically in minipigs. Radiographs of the proximal and distal epiphyses of humerus, radius, ulna, femur and tibia were obtained at 4, 8, 12, 20, 40, 48, 96 and 144 weeks of age in total 58 minipigs. The assessment of maturity process was made in accordance with the criteria proposed by Owada and Sutow. The secondary ossification centers developed rapidly from 4 weeks of age to 40 weeks of age, and gradually thereafter until 96 weeks of age. The earliest epiphyseal fusion was apparent in the proximal radius, proximal and distal femur at 96 weeks of age. The complete fusion of the epiphyseal line in the long bones was evident on 144 weeks of age and was observed in most long bones such as the proximal humerus, the proximal and distal ulna and the distal radius, and the proximal tibia in minipigs.
Partial Purification and Quantification of Insulin-like Growth Factor-I from Red Deer Antler
Gu, Lijuan ; Mo, Eun-Kyoung ; Fang, ZheMing ; Sun, BaiShen ; Zhu, XueMei ; Sung, Chang-Keun ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1321~1329
DOI : 10.5352/JLS.2007.17.10.1321
Deer antler tissue contains the most rapidly growing bone in the animal kingdom. Thus, it is likely that growing antler tissue is a rich source of local paracrine bone-stimulating factors. Growth factors, at least the insulin-like growth factor (IGF), control the bone-remodelling process. In this study, we tried to isolate and purify IGF-I from fresh antler tissue by the routine isolation and purification of protein. The purification involved ammonium sulfate precipitation, DEAE-Sepharose CL-60 ion-exchange chromatography, CM-Sepharose CL-6B ion-exchange chromatography, and Sephadex G-50 chromatography. Purified fractions from each step were analyzed by high-performance liquid chromatography (HPLC), SDS polyacrylamide gel electrophoresis (SDS-PACE), Dot-blot, and Western-blot methods. Furthermore, the quantification of partially purified IGF-I was calculated by enzyme-linked immunosorbent assays (ELISA) using antibody to human recombinant IGF-1. SDS-PAGE analysis of the final fraction yielded two molecular bands and the signal band was at 12 kDa on the Western-blot film. This purified IGF-I fraction showed a peak at retention time of eight min. The quantity of IGF-I in 20 g deer antler tissue as starting weight was calculated using a standard curve to be 2910 ng/ml, and total IGF-I amount is 0.291 g. The results show that IGF-I, which can be found in deer antler, can be partially purified and quantified by classic protein isolation methods.
Antioxidative Activity of Extracts of Aged Black Garlic on Oxidation of Human Low Density Lipoprotein
Yang, Seung-Taek ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1330~1335
DOI : 10.5352/JLS.2007.17.10.1330
This study was developed to assess the antioxidative activity of aged black garlic extract on lipid peroxidation and low density lipoprotein (LDL). Antioxidative activity of aged black garlic extract on human low density lipoprotein (LDL) was investigated by monitoring a barbituric acid reactive substance (TBARS). Electron donating ability (EDA) of aged black garlic, ethanol extract was higher than that alliin and water extract. Aged black garlic water and ethanol extracts inhibited the
mediated oxidation of human LDL in a dose dependent manner at concentration of 10 and
. Ethanol extract and water extract of aged black garlic almost completely inhibited J774 mediated LDL oxidation in electrophoretic mobility and conjugated diene. These results indicate that aged black garlic might play a protective antioxidant effects on LDL, probably affecting the structural properties for the LDL oxidation.
Efficient Bulblet Regeneration and Growth from Bulb Scale of Hyacinthus orientalis L. cv. Pink Pearl Cultured in vitro
Lee, Kyung-Soon ; Chung, Chung-Han ; Chung, Yong-Mo ; Kim, Doh-Hoon ; Jeong, Soon-Jae ; Nam, Jae-Sung ; Kim, Gyung-Tae ; Yi, Young-Byung ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1336~1340
DOI : 10.5352/JLS.2007.17.10.1336
The regeneration and growth of bulblets from the bulb scale segments of Hyacinthus orientalis L. cv. Pink Pearl were more efficient in IBA than IAA at the same concentrations (1.0 and 3.0 mg/l). The normal (base-down) orientation of explants was more effective for bulblet regeneration and root growth than the inverted (base-up) orientation. The growth of bulblets and roots was increased higher in the perlite than the agar medium. These results suggested that the alternate culture system, first cultured in the agar medium for bulblet regeneration, and then in the perlite medium for bulblet growth, may be more useful for efficient in vitro culture of hyacinth (H. orientalils) cv. Pink Pearl.
Purification and Characterization of β-Xylosidase from Paenibacillus sp. DG-22
Lee, Tae-Hyeong ; Lim, Pyung-Ok ; Lee, Yong-Eok ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1341~1346
DOI : 10.5352/JLS.2007.17.10.1341
from Paenibacillus sp. DG-22 was purified to homogeneity by ion-exchange, hydrophobic interaction and gel-filtration chromatography. The molecular weight of the enzyme was measured to be 156,000 by gel filtration and 80,000 by SDS-PAGE, indicating that the enzyme consisted of two identical subunits. The purified enzyme exhibited maximum activity at
and pH 5.5. It retained 89% of its initial activity up to 60 min at
and had a half-life of 25 min at
. The enzyme was highly specific for pNPX as the substrate. It showed little or no activity against other p-nitrophenyl glycosides and xylans. The
for pNPX was 0.53 mM and 3.18 U/mg protein, respectively. The
was strongly inhibited by
and slightly activated by DTT. The hydrolysis product from xylobiose, xylotriose, and xylotetraose was xylose.
Changes of Glycosylation Pattern in Aging Rat Kidneys as Revealed with Lectin Conjugates
Gil, Young-Gi ; Kim, Keun-Ha ; Choi, Byung-Tae ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1347~1353
DOI : 10.5352/JLS.2007.17.10.1347
The changes of glycoconjuagates (GCs) in rat kidney due to maturation were studied from samples of fetal and postnatal kidneys by lectin histochemistry. Rat kidneys of perinatal ages and adults were fixed in 4% paraformaldehyde and were stained with nine kinds of biotinylated lectins. The immature forms of the renal developmental stage such as vesicles and ureteric bud were observed in the cortex as late as day 14 of postnatal life, but the histological appearance of the weaning kidney was similar to that observed in adults. As for histochemical properties of GCs in the glomeruli, Con A affinity tended to increase with aging, but both RCA-1 and LCA affinities showed a transient increase in immature glomeruli of neonatal rats. DBA affinity with SBA, PNA, BSL-1 and RCA-1, additional Con A one in proximal tubule, were increased in both proximal and distal tubules according to maturation. In contrast to this, transient intensive LCA affinity were demonstrated in immature proximal and distal tubule of neonatal rats. In the collecting tubules, DBA, SBA, PNA and sWGA affinities tended to increase according to maturation, but transient increase for BSL-1, RCA-1 and LCA affinities were detected in neonatal rats. The present results suggest that the mature glycosylation pattern of the kidney undergoes profound changes during maturation and is probably associated with functional maturation of the kidney.
Effect of Tea Polyphenols on Anticancer Activity and Cytokines Production
Shon, Mi-Yae ; Nam, Sang-Hae ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1354~1360
DOI : 10.5352/JLS.2007.17.10.1354
Theaflavins (TF) and thearubigins (TR) are constituents of tea pigments which are polyphenols derived from Korean fermentation tea. After TF, TR and [(-) epigallocatechin-3-gallate](EGCG) have been applied to macrophage cell line (RAW264.7) nitric oxide (NO) synthesis and cytokines production were estimated. Cytokines production by enzyme linked immune-sorbent assay (ELISA) determined. NO production was increased by about 1.5-folds at the dose of
compared to control and lipopolysaccharide (LPS) stimulation when TF, TR and EGCG were applied to a RAW264.7 cell. Interleukin-6 (IL-6), Tumor necrosis factor (
) and granulocyte-macrophage colony stimulating factor (GM-CSF) increased depended on concentrations of TF, TR and EGCG. The production of tumor necrosis
increased highly in TR, TF and EGCG group with LPS. These results suggest that TF, TR and EGCG have immune-enhancement effect through the cytokine production. TF, TR and EGCG inhibited cancer cell viability, the anticancer effect of these polyphenols may explain the anti-tumor promotion action and antioxidant activity of these tea constituents.
A Protein Kinase-A Inhibitor, KT5720, Suppressed Cytopathic Effect Caused by Vesicular Stomatitis Virus
Kim, Young-Sook ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1361~1367
DOI : 10.5352/JLS.2007.17.10.1361
I investigated the effect of KT5720, an inhibitor of protein kinase A, on the vesicular stomatitis virus (VSV) infection in BHK-21cell cultures. The virus inducted cytopathic effect (CPE) was almost completely suppressed by KT5720 at 5uM. The inhibitor, however, did not affect replication of the virus nor the synthesis of viral macromolecules. KT5720, did not block the cytoskeletal disruption, while the cell rounding was suppressed. And, the KT5720-sensitive function may be involved in developing the VSV-induced CPE, but not essential for the virus replications.
Antimutagenicity and Anticancer Activity of Soybean Fractions Extracted by Solvents
Lim, Sun-Young ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1368~1373
DOI : 10.5352/JLS.2007.17.10.1368
Inhibitory effects of several solvent fractions from soybean on mutagenicity using Salmonella typhimurium TA 100 in Ames test and growth of human cancer cells (AGS gastric adenocarcinoma, Hep 3B hepatocellular cancinoma and HT-29 colon cancer cells) were studied. The treatment of dichloromethane and ethylacetate fractions (2.5 mg/assay) extracted from soybean to Ames test system inhibited aflatoxin
induced mutagenicity by 83%, respectively, and showed a higher antimutagenic effect than other solvent fractions. In case of N-methyl-N#-nitro-N-nitrosoguamidine (MNNG) induced mutagenicity, the ethylacetate fraction showed the highest inhibitory effect (by 67%) among solvent extracts, although the inhibitory effect was not stronger compared with
induced mutagenicity. In sulforhodamine B (SRB) assay, the treatment of ethylacetate fraction (2 mg/assay) significantly inhibited the growth of AGS, Hep 3B and HT-29 cancer cells by 66%, 73% and 77%, respectively, followed with the intermediate and dichloromethane fractions. These results indicated that soybean fraction extracted with ethylacetate had higher inhibitory effects on
and MNNG in Ames test and growth inhibition activity to human cancer cells was appeared, suggesting that soybean fraction extracted with ethylacetate may contain the biologically active compounds.
Detection and Distribution of Bacterial Pathogens in Raw Water and During Water Treatment Process by Polymerase Chain Reaction
Park, Hong-Ki ; Jung, Eun-Young ; Jung, Jong-Moon ; Yu, Pyung-Jong ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1374~1380
DOI : 10.5352/JLS.2007.17.10.1374
The development of polymerase chain reaction (PCR) technology has the potential to solve for isolating pathogenic microorganisms from environmental samples than traditional plate counting methods. We have been detected pathogenic bacteria from raw water and water treatment process in Busan metropolitan city by PCR method. According to the result of survey from July 2004 to October 2005, 80 out of 92(87.0%) were positive for bacterial pathogens in raw water samples and positive rate of Shigella spp., Yersinia spp., Salmonella spp. and Legionella spp. were 46.2%, 40.7%, 17.6% and 9.9%, respectively. Pathogenic bacteria in raw water was mainly distributed through the lately Autumn to the winter and more highly detected Maeri than Mulgum region. During the period of survey in water treatment process, Shigella spp. was highly detected but all of bacterial pathogens were entirely removed after in post-ozonation step. These suggest that waters supplied in Busan metropolitan city may be safe against the pathogenic bacteria.
Changes in Physiological Activities of Scutellariae baicalensis by Heating
Choi, Myeong-Rak ; Lee, Jin-Seung ; Lim, Hyun-Soo ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1381~1386
DOI : 10.5352/JLS.2007.17.10.1381
To elucidate the changes in physiological activities of Scutellariae baicalensis by heating, composition analysis with HPLC and evaluation of physiological activities of extracts were conducted. HPLC analysis of CW(water extract at room temperature) and CHW(heat treated CW) indicated that two distinctive peaks in the HPLC chromatogram were disappeared after boiling and additional peak was newly observed. This observation suggested that certain chemical changed in the extract occurred by heat treatment. In the estimation of physiological activities, CW showed higher ACE inhibiting activity than CHW. In the case of cytotoxic activity against cancer cells, CW exhibited higher activity than CHW against CCL-185. On the other hand, CHW showed higher activity than CW against HeLa, SNU-601 and HepG2. In addition, CHW showed the inhibitory effect on melanogenesis (80% inhibition) while CW did not show any activity.
Effects of Hambag Mushroom (Grifola frondosa)-Powder on Hyperglycemia and Hyperlipemia in STZ and High Fat Diet-induced Diabetic Rats
Lee, Soon-Lee ; Park, Yeong-Chul ; Kim, Jong-Bong ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1387~1393
DOI : 10.5352/JLS.2007.17.10.1387
Hambag mushroom is edible mushroom, and one of new strains in Grifola frondosa. It was developed from Korean wild Grifola frondosa by National Institute Science & Technology. In this study, we investigated the effects of the powder extracted from fruit-body of hambag mushroom on hyperglycemia, hyperlipemia and pancreatic cells in rats with streptozotocin (STZ) and high fat diet-induced diabetes. The diabetic rats were fed with hambag mushroom-powder (1 and 2% of diet weight) for 6 weeks. And then the levels of blood glucose and lipid and the pancreatic tissue were analyzed and compared. The diabetic rats fed with hambag mushroom-powder lost less body weight than those fed with the powder-free normal diet. flood glucose levels in the diabetic rats fed with hambag mushroom-powder at all concentrations was lower by 50% than in the diabetic rats fed with normal diet. The levels of total cholesterol, triglyceride and LDL-cholesterol in the diabetic rats fed with hambag mushroom-powder were increased compared to those in the diabetic rats fed with normal diet. There were the blood levels of cholesterol, triglyceride and LDL-cholesterol by hambag mushroom-powder concentrations in a dose-dependent response. The decreased damage of pancreatic tissue in the group treated with hambag mushroom-powder could be suggested possibly as one of mechanisms for the effects of hambag mushroom-powder on hyperglycemia and hyperlipemia.
Studies on Saprogenic Bacteria Isolated from Korean Red Ginseng Extrart Product
Kim, Young-Man ; Yoon, Choong-Eui ; Kim, Kyoung-Hee ; Lee, Eun-Woo ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1394~1399
DOI : 10.5352/JLS.2007.17.10.1394
Five kinds of saprogenic bacteria were isolated from the red ginseng extract product and identified as Bacillus subtilis, Bacillus sp., Paenibacillus sp., Micrococcus sp., and Pseudomonas sp. by 16S rDNA analysis. Some of the isolated strains were able to grow even at
which are presumed originated from the raw ingredient of red ginseng extract. All of the isolated strains did not show the hemolytic activity, the diarrhea-inducing activity, and the vascular permeability enhancing activity, indicating that these strains are not pathogenic.
Effect of Supplementation with Docosahexaenoic Acid from Gestation to Adulthood on Spatial Learning Performance in Rat
Lim, Sun-Young ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1400~1405
DOI : 10.5352/JLS.2007.17.10.1400
The effect of supplementation with docosahexaenoic acid into n-3 fatty acid deficient diet on improvement of loaming related brain function was investigated. On the second day after conception, Sprague Dawley strain dams were subjected to a diet containing either n-3 fatty acid deficient (Def) or n-3 fatty acid deficient + docosahexaenoic acid (Def+DHA). After weaning, male pups were fed on the same diet of their respective dams until adulthood. Motor activity and Morris water maze tests were measured at 10 weeks old. In motor activity test, there were no statistically significant differences in moving time and moving distance between the Def and Def+DHA diet groups. The n-3 fatty acid deficient with DHA (Def+DHA) group exhibited a shorter escape latency, swimming time and swimming distance (P<0.05) compared to the n-3 fatty acid deficient group (Def) but there was no difference in resting time and swimming speed between the experimental diet groups. In memory retention trial, the number of crossing of the platform position (region A) was significantly greater than those of other regions for the Def+DHA group. However, the Def group swam randomly without preference for the provisions platform location, indicating poorer memory retention. From those results, supplementation with DHA into the n-3 fatty acid deficient diet improved the spatial loaming ability in rats as assessed by Morris water maze test.
Effect of Pimpinella Brachycarpa Ethanol Extract on Chronically Ethanol-Induced Liver Damage in Rats
Choo, Myung-Hee ; Lee, Jae-Joon ; Lee, Myung-Yul ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1406~1413
DOI : 10.5352/JLS.2007.17.10.1406
This study was performed to investigate the effect of ethanol extract of Pimpinella brachycarpa(PBE) on chronically ethanol-induced hepatotoxicity in rat liver. Sprague-Dawley rats weighing 90-130 g were divided into 5 groups; normal group(NOR), ethanol(35%, 10 ml/kg) treated group(CON), PBE 200 mg/kg treated group(P1), PBE 200 mg/kg and ethanol treated group(P2), and PBE 400 mg/kg and ethanol treated group(P3). PBE was also fractionated by the following solvent: n-hexane, chloroform, ethylacetate and n-butanol. The antioxidative capacity of the n-hexane fraction was the highest among fractions and was similar to that of butylated hydroxytoluene(BHT). The body weight gain and feed intake of the rats were decreased by ethanol administration, but were gradually increased to the similar levels of the NOR group by administering PBE. The AST activity in serum elevated by ethanol was significantly decreased by administering the high dosage of PBE, but exerted no significant change on serum ALT activity. It was also observed that the hepatic activities of xanthine oxide(XO), catalase and glutathione peroxidase(GSH-Px) increased by ethanol were markedly decreased in the combined ethanol and PBE administered groups(P2 and P3), but not in the activity of superoxide dismutase(SOD) as compared with the CON group. The glutathione(GSH) contents were decreased by ethanol adminstration, however, increased after administering PBE. These results suggest that ethanol extract of Pimpinella brachycarpa has a possible positive effect on the liver function in hepatotoxicity-induced rats by ethanol administration.
Accumulated Concentration of Lead in Plant Organ of Arabidopsis thaliana Exposed to Lead
Park, Jong-Bum ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1414~1418
DOI : 10.5352/JLS.2007.17.10.1414
This study was to examine the accumulated concentration of lead in the organ of Arabidopsis thaliana grown in the soil added three different concentrations of lead. The accumulated concentrations of lead in the stem of plant grown in the soil added official standard concentration of lead of pollutant exhaust notified by the Ministry of Environment (1 mg/l), concentration ten times higher than the official standard concentration (10 mg/l) and concentration fifty times higher (50 mg/l) were similar to the rate of increase between three different concentrations, and increased average 24% compared with normal plant stem. The accumulated concentrations of lead in the leaf of plant grown in the soil added three different concentrations of lead were increased average 57% compared with normal plant leaf. And accumulated concentrations of lead in the leaf was no significant difference according to increase of lead concentration added in the soil as stem, the rate of increase was similar to between three different concentrations. The accumulated concentrations of lead in the root of plant grown in the soil added official standard concentration of lead of pollutant exhaust and concentration ten times higher were increased average 114% compared with normal plant root, but increased about 861% in the concentration fifty times higher than the official standard concentration. This result contrast with the data of stem and leaf. The accumulated concentration of lead in the plant body of Arabidopsis thaliana was increased according to increase of lead concentration added in the soil. Especially, the accumulated concentration of lead in the plant body grown in the concentration fifty times higher than the official standard concentration was increased about 2.6 times than normal plant. These results show that lead contaminated within the soil was more accumulated in the root than the stem or leaf, and accumulated concentrations of lead in the stem and leaf were not increased in proportion to the concentration of lead in the soil, but very increased in proportion to the concentration in the root.
The Effect of 12 Weeks Combind Exercise on C-reactive protein, Adiponectin and Insulin Resistance in Obese Middle School Girls
Jeon, Jae-Young ; Kwak, Yi-Sub ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1419~1425
DOI : 10.5352/JLS.2007.17.10.1419
The purpose of the present investigation was to determine the effect of combine exercise on C-reactive protein(CRP) and adiponectin, and to asses whether combine exercise-induced changes in insulin resistance could be explained in part by changes in these in these inflammation markers. Twenty two participants (BMI >95 percentile for age and sex) were allocated exercise group(n=12) and control group(n=10). Subjects had their body shape, body composition, glucose. insulin, HOMA-IR, CPR and adiponectin levels measured. Modest improvements in body composition, insulin resistance markers were observed, however, adiponectin and CRP did not changes. These data suggest that adiponectin and CRP is not a contributory factor to the exercise-relateted improvements in insulin resistance. Additional studies are needed to assess the effects of different duration, modes and intensities of exercise on inflammation markers.
Analysis of Antibiotic Resistant Patterns in Conjugant and Transformant of Three ESBL gene Harboring Klebsiella pneumoniae
Kim, Yun-Tae ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1426~1433
DOI : 10.5352/JLS.2007.17.10.1426
To investigate the antibiotic resistant patterns of the bacteria producing ESBL, we isolated one organism of Klebsiella pneumoniae from a clinical laboratory in Busan. The organism that produces ESBL gene was detected by double disk synergy test and the presence of three ESBL genes (TEM-1, SHV-12, CTX-M-15) was confirmed by polymerase chain reaction and DNA sequencing analysis. To analyse the characteristics of three ESBL genes, we performed transconjugation, transformation and cloning experiment with the organism. The MIC of Klebsiella pneumoniae was revealed that ceftazidime, cefotaxime and ceftriaxone were
respectively. The MIC of conjugant (E. coli
) af was revealed that ceftazidime, cefotaxime and ceftriaxone were
respectively. The MIC of transformant (E. cofi
) was revealed that ceftazidime, cefotaxime and ceftriaxone were
respectively, The MIC of cloned organism of SHV-12 gene (E. coli
) was revealed that ceftazidime, cefotaxime and ceftriaxone were
respectively. The results indicated that MIC of conjugant was higher than MIC of transformant and also SHV-12 gene were not resistant against cefotaxime antibiotic.
Effect of Culture Broth from Mushroom Mycelium on Growth and Non-specific Immune Parameters in Flounder (Paralichthys olivaceus) by Oral Administration
Kim, Man-Chul ; Kim, Min-Joo ; Kim, Ju-Sang ; Heo, Moon-Soo ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1434~1440
DOI : 10.5352/JLS.2007.17.10.1434
We have investigated the effects of mushroom mycelium (Phellinus linteus, Coriolus versicolor) mixed cultural extract on the immune responses of olive flounder, Paralichthys olivaceus. The mixed culture extracts were evaluated for the growth, hematology, lysozyme activity, leukocyte phagocytic activity, and disease resistance against Vibrio anguillarum. In the effect of the growth, the body weight and length gain in the group, which fed with mushroom mycelium mixed cultural extract, were 52 g and 3.4 cm higher than that in the control, respectively. For the hematology, the administration of mushroom mycelium mixed cultural extract resulted in increase of glucose. However, there was no distinct differences in GOT (glutamic oxaloacetic transaminase), GPT (glutamlc pyruvic transamlnase), TG, TP, and LDH (lactate dehydronase) among each group. The activities of lysozyme were 80% higher in the experimental groups than in the control. The activities of leucocyte were 66% higher in the experimental groups than in the control. Although lysozyme activity and leucocyte activity showed somewhat decrease after 12 weeks, these activities were still higher than in the control. The cumulative mortality (%) after an artificial challenge with
of Vibrio anguillarum per fish was 25% higher in the experimental groups than the control.
Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite Loci in Jeju Native Horse
Cho, Byung-Wook ; Jung, Ji-Hye ; Kim, Sang-Wook ; Kim, Heui-Soo ; Lee, Hak-Kyo ; Cho, Gil-Jae ; Song, Ki-Duk ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1441~1446
DOI : 10.5352/JLS.2007.17.10.1441
This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be
. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.
Apoptotic Cell Death by Pectenotoxin-2 in p53-Deficient Human Hepatocellular Carcinoma Cells
Shin, Dong-Yeok ; Kim, Gi-Young ; Choi, Byung-Tae ; Kang, Ho-Sung ; Jung, Jee-H. ; Choi, Yung-Hyun ;
Journal of Life Science, volume 17, issue 10, 2007, Pages 1447~1451
DOI : 10.5352/JLS.2007.17.10.1447
Through the screening of marine natural compounds that inhibit cancer cell proliferation, we previously reported that pectenotoxin-2 (PTX-2) isolated from marine sponges exhibits selective cytotoxicity against several cell lines in p53-deficient tumor cells compared to those with functional p53. However, the molecular mechanisms of its anti-proliferative action on malignant cell growth are not completely known. To further explore the mechanisms of its anti-cancer activity and to test whether the status of p53 in liver cancer cells correlates with their chemo-sensitivities to PTX-2, we used two well-known hepatocarcinoma cell lines, p53-deficient Hep3B and p53-wild type HepG2. We have demonstrated that PTX-2 markedly inhibits Hep3B cell growth and induces apoptosis whereas HepG2 cells are much more resistant to PTX-2 suggesting that PTX-2 seems to act by p53-independent cytotoxic mechanism. The apoptosis induced by PTX-2 in Hep3B cells was associated with the modulation of DNA fragmentation factor (DFF) family proteins, up-regulation of pro-apoptotic Bcl-2 family members such as Bax and Bcl-xS and activation of caspases (caspase-3, -8 and -9). Blockade of the caspase-3 activity by caspase-3 inhibitor, z-DEVD-fmk, prevented the PTX-2-induced growth inhibition in Hep3B cells. Moreover, treatment with PTX-2 also induced phosphorylation of AKT and extracellular-signal regulating kinase (ERK), but not c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MARK). Specific inhibitors of PI3K inhibitor (LY294002) and ERK1/2 inhibitor (PD98059) significantly blocks PTX-2-induced-anti-proliferative effects, whereas a JNK inhibitor (SP600125) and a p38 MAPK inhibitor (SB203580) have no significant effects demonstrating that the pro-apoptotic effect of PTX-2 mediated through activation of AKT and ERK signal pathway in Hep3B cells.