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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
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Genetic Evolution and Characteristics of Ichthyotoxic Cochlodinium polykrikoides(Gymnodiniales, Dinophyceae)
Cho, Eun-Seob ; Jeong, Chang-Su ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1453~1463
DOI : 10.5352/JLS.2007.17.11.1453
This study presents a molecular phylogenetic analysis of the harmful dinoflagellate Cochlodinium polykrikoides, by use of partial sequence of small subunit (SSU) rRNA gene from most of the major taxa(24 species) in dinoflagellates. The class Dinophyceae clade formed a strong monophyletic relationship with C. polykrikoides and several taxa. On the basis of deeper nodes, the phylogenetic relationships placed C. polykrikoides closer to the order Prorocentrales rather than to the order Gymnodiniales, which was supported by a strong bootstrap value (100%) in the analyses of Neighbor-Joining and Parsimony methods. There is strong support for C. polykrikoides being placed in the same branch as Gymnodiniaceae and being connected in a clade with Prororcentrum micans among Prorocentrales. Morphological data show that C. polykrikoides is well associated with the genus Gyrodinium; however, this species is genetically closer to Gymnodinium than to Gyrodinium. The placement of C. polykrikoides always formed an independent branch separated from other dinoflagellates. In conclusion, planktonic P. micans plays an important role as an ancestor of Gymnodinium, whereas C. polykrikoides appears to be used an intermediate position between P. micans and Gymnodinium based on evolution.
Effects of Treadmill Exercise on Memory and Hippocampal BDNF Expression in Streptozotocin-induced Diabetic Rats
Lee, Hee-Hyuk ; Yoon, Jin-Hwan ; Kim, Seung-Hee ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1464~1471
DOI : 10.5352/JLS.2007.17.11.1464
Diabetes mellitus is a chronic metabolic disorder, leading to many complications including cognitive deficit. Regular exercise has often been recommended as a therapeutic maneuver to the diabetic patients for the prevention of secondary complications. In the present study, the effects of treadmill exercise on memory and brain-derived neurotrophic factor (BDNF) in the hippocampus of streptozotocin (STZ)-induced diabetic rats were investigated. Male SD rats, aged 6 weeks, were randomly assigned to the following three groups: control group(n
The Influence of Cranial Cervical Ganglion Block in Beagle Dogs with Normal Intraocular Pressure
Park, Woo-Dae ; Bae, Chun-Sik ; Kim, Hwi-Yool ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1472~1476
DOI : 10.5352/JLS.2007.17.11.1472
This study was performed to observe changes of the intraocular pressure following cranial cervical ganglion block with the four different concentrations of lidocaine in beagle dogs with the normal intraocular pressure. We performed by the crossover test in ten beagle dogs divided into four groups, which were 2%, 1%, 0.5% and 0.25% lidocaine group. All experimental dogs received each four times cranial cervical ganglion block using 2 ml of lidocaine with the four different concentrations. The blocks were separately done at a week intervals in random order. Horner#s syndrome was observed in all groups. The intraocular pressure in the blocked side was significantly increased to 5 min in the 1% lidocaine group compared to the baseline (p<0.05). The intraocular pressure of 2% and 1% lidocaine groups were decreased between 25 and 45 min following cranial cervical ganglion block, while those of 0.5% and 0.25% lidocaine groups were unchanged. In conclusion, these results suggest that cranial cervical ganglion block affect on the change of intraocular pressure and 0.5% and 0.25% lidocaine are inadequate concentrations for the change of intraocular pressure in the dog with normal intraocular pressure.
Mating Systems and Inbreeding Pressure in Populations of Wild Lentil Tare, Vicia tetrasperm (Leguminosae)
Huh, Man-Kyu ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1477~1481
DOI : 10.5352/JLS.2007.17.11.1477
The mating systems of natural populations of Vicia tetrasperm in Korea were determined using allozyme analysis. The result suggests that V. tetrasperm is low rates of outcrossing or mix-mating outcrossing (self-fertilization, s < 0.5). At the population levels, the values of inbreeding coefficient of ten populations in Korea varied from 0.131 to 0.176, giving an average 0.154. For ten natural populations, multi-locus estimates of outcrossing (tm) was 0.333 across fifteen polymorphic loci, with individual population values ranging from 0.269 to 0.423. The differences between the tm and ts values were not close to zero (tm - ts > 0.154), indicating that biparental inbreeding was significant in the loci. The reason for relatively low outcrossing rates of some populations could be attributed to extensive consanguineous mating and isolation of flowering mature plants. Although heterozygote excess was observed in one natural population, most populations exhibited varying degrees of inbreeding and heterozygotes deficit. Thus, selection against homozygotes operated in the progeny populations throughout the life cycle.
Genetic Diversity and Phylogenetic Relationship of Genus Phyllostachys by ISSR Markers
Lee, Song-Jin ; Huh, Man-Kyu ; Huh, Hong-Wook ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1482~1487
DOI : 10.5352/JLS.2007.17.11.1482
Four taxa of the genus Phyllostachys were analysed with PCR-based molecular inter-simple sequence repeat (ISSR) determine markers to determine their phylogenetic relationships. Many species of this genus are regarded as economical and ecologically important in the world. With the nine primers screened, ISSR assay generated a total of 64 reproducible bands. Analysis of ISSR from individual plants of genus Phyllostachys resulted in 43 polymorphic bands with 70.49%. When species were grouped by four taxa, within group diversity was 0.092 (Hs), while among group diversify was 0.499 (Gst) on a per locus basis. The estimated gene flow (Nm) between the pairs of species was 0.559. Hence, we can expect weak or low gene flow among species. Total mean genetic diversity (
) for four taxa was 0.291. The ISSR data allowed us to resolve well-supported clades in the genus Phyllostachys. The four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic.
The Ossifying Epulis Accompanying Multi-Nucleated Giant Cells in a Dog
Hong, Il-Hwa ; Jeong, Won-Il ; Son, Young-Sook ; Park, Jin-Kyu ; Yang, Hai-Jie ; Yuan, Dong-Wei ; Goo, Moon-Jung ; Lee, Hye-Rim ; Jeong, Kyu-Shik ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1488~1491
DOI : 10.5352/JLS.2007.17.11.1488
An epulis was occurred on gingiva of 11-year old female dog, Yorkshire terrier. Our case had feature of ossifying epulis but there were a few multi-nucleated giant cells (MGCs). MGCs had osteoclast-like appearance and giant cell epulis usually appears at the site of tooth extraction. Therefore, we suggest that appearance of MGCs in our case may be due to phagocytosis pre-formed osteoid/bone or our case may be mixed epulides of ossifying and giant cell epulis by mixed stimulation of chronic gingivitis and trauma and in flammation by tooth extraction. Thus, MGCs have possibility enough to appear in ossifying epulis, but ossifying epulis accompanying MGCs has not been reported. Therefore, our case may deserves an attention as an unique case and will be helpful to study pathogenesis of giant cell containing lesion of the jaw.
Induction of Rice Allergen-Specific IgE Synthesis by KU8l2 Cells
Shim, Sun-Yup ; Katakura, Yoshinori ; Shirahata, Sanetaka ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1492~1496
DOI : 10.5352/JLS.2007.17.11.1492
In vitro IgE class switching could be induced through co-culture of CD40L-expressing KU812 cells and CD40-expressing B cells in the presence of IL-4 or IL-13. It has been generated several B cell lines, which produce rice allergen (RA)-specific IgM antibody by in witγo immunization (IVI) using peripheral blood lymphocyte (PBL). In this study, induction of RA-specific IgE antibody by KU812 cells was attempted. Before co-culture, we determined the CD40 expression in RA-specific B cell lines, RA9G11 and the CD40 ligand (CD40L) expression in activated KU812 cells by treatments with phorbol myristate acetate (PMA) and ionomycin for 6 hrs. Flow cytometric analysis shown that RA9G11 and activated KU812 cells expressed high level of CD40 and CD40L, respectively. RA9G11 cells were cultured with activated KU812 cells for 12 days in the presence of IL-4 for IgE class switching. Mature
mRNA level and RA-specific IgE spot forming cells (SFC) were observed in all culture condition, and especially, high level of RA-specific IgE synthesis was determined the same ratio of RA9G11 and activated KU812 cells in the presence of 50U IL-4. Therefore, induction of RA-specific IgE synthesis by activated KU812 cells can be contributed in the application for allergic therapy and prevention.
Biological Activity of Recombinant Human Thrombopoietin
Kim, Boing-Soon ; Naidansuren, Purevjargal ; Min, Kwan-Sik ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1497~1504
DOI : 10.5352/JLS.2007.17.11.1497
To investigate the function and secretion of human thrombopoietin (TPO) in mammalian cells, hTPO cDNA was cloned using human liver cDNA, and recombinant hTPO (rec-hTPO) was produced in CHO cell lines. In addition, six N-linked glycosylation sites were substituted for Ala to elucidate the role of each carbohydrate chain. To analyze the biological activity, rec-hTPO protein was injected subcutaneously. Blood was withdrawn for platelet determination. The metabolic clearance rate (MCR) was also analyzed at the 1, 4, 10 and 24 hr after tail vein injection. Wild-type TPO (WT) was efficiently secreted into the medium. However, a hTPO mutant with 116 deleted nucleotides detected by PCR cloning was not secreted. The N-linked glycosylation sites had nearly the same expression quantity as rec-hTPO WT apart from mutants 3 and 4. The glycosylation site of mutant 4 appeared to be an indispensable site for hTPO secretion. Also characterized was the biological activity through an injection with rec-hTPO (10 ng) to ICR mice (7 weeks). The result of the blood analysis showed a considerable increase in the platelet number six days after He injection. To analyze the pharmacokinetics, rec-hTPO was injected into the tail vein (5 ng). The result was 200 pg/ml 1hr after this injection. Following this, it dramatically decreased and virtually disappeared 10 hours after the injection. Thus, rec-hTPO may be a treatment for thrombopenia by the production of the high active rec-hTPO. In addition, hTPO can permit the development of potent new analogues that stimulate the platelet value.
Investigation of Single Nucleotide Polymorphisms in the Adipocyte Fatty-Acid Binding Protein (FABP4) Gene
Kim, Sang-Wook ; Jung, Ji-Hye ; Kim, Kwan-Suk ; Lee, Cheol-Koo ; Kim, Jong-Joo ; Choi, Bong-Hwan ; Kim, Tae-Hun ; Song, Ki-Duk ; Cho, Byung-Wook ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1505~1510
DOI : 10.5352/JLS.2007.17.11.1505
We found 8 single nucleotide polymorphisms (SNPs) in adipocyte fatty acid bonding protein (FABP4) gene as candidate gene of FAT1 locus on pig chromosome 4. With over 800 heads of major commercial pig breeds including Duroc, Landrace, Berkshire and Yorkshire, we analyzed SNPs of FABP4 gene to determine possible effects of FABP4 genotype to economically important traits.
amplicons in FABP4 gene were used PCR-RFLP for each SNPs and we found that the frequency of some SNPs of this gene was different among the breeds. According to the statistical analyses to determine possible associations of each genotype with economic traits, it was found that subgroup with different genotypes showed significant differences in daily gain, backfat thickness, lean percentage and feed conversion ratio (P<0.05). Thus, as a Part of enhancing the selection competence related to swine growth rate and lean percentage, it is expected that FABP4 gene markers verified in this study will be useful to use for Korean commercial pig industry.
Inhibitory Effects of Acanthopanax chiisanensis Ethanolic Extracts on FcεRI α Chain Expression
Shim, Sun-Yup ; Sung, Chan-Ki ; Lee, Sang-Won ; Choi, Young-Ju ; Kim, Hyeung-Rak ; Byun, Dae-Seok ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1511~1516
DOI : 10.5352/JLS.2007.17.11.1511
Basophils and mast cells play an important role in
allergic reaction as effector cells. We studied the effects of Acanthopanax chiisanensis on
chain expression in human basophilic KU812F cells. Ethanol extracts from root and stem of A. chiisanensis were tested for inhibitory effects of
chain expression. The cell surface
chain expression was examined by flow cytometric analysis. All of the extracts of A. chiisanensis reduced the cell surface
chain expression. Furthermore, A. chiisanensis extracts caused a decrease in the level of
chain mRNA level and
histamine release. These results suggest that root and stem extracts of A. chiisanensis play an important role in anti-allergic activity via down-regulation of
chain expression and decrease in release of inflammatory mediator such as histamine.
Single Channel Analysis of Xenopus Connexin 38 Hemichannel
Cheon, Mi-Saek ; Oh, Seung-Hoon ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1517~1522
DOI : 10.5352/JLS.2007.17.11.1517
Gap junction channels formed by two adjacent cells allow the passage of small molecules up to
between them. Hemichannel (connexon or half of gap junction) also behaves as a membrane channel like sodium or potassium channels in a single cell membrane. Among 26 types of connexin (Cx),
(a chimera in which the first extracellular loop of Cx32 has been replaced with that of Cx43), Cx38, Cx46, and Cx50 form functional hemichannels as well as gap junction channels. Although it is known that Xenopus oocytes express endogenous connexin 38 (Cx38), its biophysical characteristics at single channel level are poorly understood. In this study, we performed single channel recordings from single Xenopus oocytes to acquire the biophysical properties of Cx38 including voltage-dependent gating and permeation (conductance and selectivity). The voltage-dependent fast and slow gatings of Cx38 hemichannel are distinct. Fast gating events occur at positive potentials and their open probabilities are low. In contrast, slow gatings dominate at negative potentials with high open probabilites. Based on hi-ionic experiments, Cx38 hemichannel is anion-selective. It will be interesting to test whether charged amino acid residues in the amino terminus of Cx38 are responsible for voltage gatings and permeation.
Ginseng Saponin-Re and Coix lachrymajobi var. mayuen Regulate Obesity Related Genes Expressions, TNF-alpha, Leptin, Lipoprotein Lipase and Resistin in 3T3-L1 Adipocytes
Kim, Sung-Ok ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1523~1532
DOI : 10.5352/JLS.2007.17.11.1523
In order to determine if the mRNA and protein expression levels of 3T3-L1 adipocytes are influenced by oriental medicines, adipocytes were treated with
of G-Re and aqueous extract of a Coix lachrymajobi var. mayuen (AEC) every other day for 12 days, respectively. The tumor necrosis factor alpha (
). mRNA and protein expressions were suppressed markedly in treated mature adipocytes. Those of lipoprotein lipase (LPL) levels were found to increase gradually in preadipocytes differentiating into mature adipocytes. Those were higher than that of the untreated mature adipocytes. The treated adipocytes showed reduction of leptin expression levels, while in untreated mature adipocytes cell, those of levels were significantly higher after the conversion of preadipocytes into mature adipocytes. The resistin levels in the treated adipocytes were significantly decreased comparing to that of the untreated mature adipocytes. In conclusion, the expression levels of LPL,
, leptin and resistin mRNA and proteins are shown to be regulated by G-Re and AEC, making them potential candidates for controlling fat mass related obesity.
Effect of Solvent Extracts from Sargassum hemiphyllum on Inhibition of Growth of Human Cancer Cell Lines and Antioxidant Activity
Choi, Hyung-Ju ; Seo, Young-Wan ; Lim, Sun-Young ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1533~1538
DOI : 10.5352/JLS.2007.17.11.1533
This study was carried out to determine the inhibitory effects of solvent extracts from Sargassum hemiphyllum on growth of cancer cell lines (AGS human gastric adenocarcinoma and HT-29 human colon cancer cells) and production of lipid peroxides. Inhibitory effects of acetone with methylene chloride extract from S. hemiphyllum on the growth of AGS and HT-29 cancer cells were increased as dose dependent patterns (p<0.05). The methanol extract was more effective on inhibition of growth of AGS. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of cancer cells (p<0.05) and the inhibitory effect was stronger in HT-29. In DCFH-DA (dichlorodihydrofluorescin diacetate) assay, acetone with methylene chloride and methanol extracts showed a stronger inhibitory effect on the production of cellular lipid peroxides (p<0.05) compared with the butanol and hexane fractions. These results indicate that the consumption of S. hemiphyllum may be recommended as a potent functional food for preventing cellular oxidation and cancer.
Antiinflammatory and Myocardial Protective Effects of Magnesium in Patents Undergoing Valvular Heart Surgery
Moon, Seong-Min ; Kang, Shin-Beum ; Hyun, Kyung-Yae ; Choi, Seok-Cheol ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1539~1546
DOI : 10.5352/JLS.2007.17.11.1539
We have investigated whether the supplement of magnesium to cold blood cardioplegia improves myocardial protection. Sixty patients scheduled for elective valvular heart surgery were randomly assigned to a control group (n
Growth and Antioxidant Activity on Lactic Acid Bacteria and Antimicrobial Activity on Fish Pathogenic Bacteria By Prunella vulgaris var. aleutica Fernald Extracts
Moon, Young-Gun ; Yeo, In-Kyu ; Heo, Moon-Soo ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1547~1554
DOI : 10.5352/JLS.2007.17.11.1547
In this study was investigated the growth effect of Prunella vulgaris. aleutica Fernald(leaf and flower) extracts on various lactic acid bacterias, electron donating ability and hydroxyl radical scavenging activity. The total cell count of Enterococcus faecium KCCM 12118, Lactobacillus rhamnosus KCCM 32826, Lactobacillus plantarum KCCM 11542, Pediococcus pentosaceus KCCM 40464 in the absence at
after 48hr were
. On the other hand, the total cell count of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCC 11542, P. pentosaceus KCCM 40464 in the presence of Prunella vulgaris. aleutica Fernald(leaf and flower) extracts(10%) at
after 48hr were
. The electron donating ability indicated to E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 added by 10% Prunella vulgaris. aleutica Fernald(leaf and flower) extracts, respectively. when 10% native plant extracts were added lactic acid bacterias, the electron donating ability is the highest. Hydroxyl radical scavenging activity of L. plantarum KCCM 11542, L. rhamnosus KCCM 32826, E. faecium KCCM 12118, P. pentosaceus KCCM 40464 showed higher than that of control.
Effects of Biologically Active Materials Prepared for Several Minerals and Plants on the Growth of Rumen Microbes
Shin, Sung-Whan ; Lee, Shin-Ja ; Ok, Ji-Un ; Lee, Sang-Min ; Lim, Jung-Hwa ; Kim, Kyoung-Hoon ; Moon, Yea-Hwang ; Lee, Sung-Sill ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1555~1561
DOI : 10.5352/JLS.2007.17.11.1555
In order to know the effects of scoria, germanium, charcoal, ginger, stevia, and CLA(Conjugated Linoleic Acid) as biologically active materials on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro lumen microbial growth, gas production, ammonia concentration, carboxymethyl-cellulase (CMCase) activity, and microbial populations were investigated. The growth of pathogenic microbes was inhibited by the supplement of 0.10% ginger. Ginger had powerful antimicrobial properties on all the pathogens used in this experiments. Additionally in the antibacterial assay by paper disc method, we could observe the clear zone of similar area with the positive control(antibiotics) for E. coli as applied with the 10% stevia or the 10% CLA only. The supplements of ginger, stevia and CLA in vitro rumen fermentation inhibited populations of rumen bacteria and protozoa. Particularly supplement of ginger resulted in remarkable reduction of the protozoa population, which means it might serve as a source inhibiting material of methane creation in the rumen.
Characteristics of Ju-Back and Effect of Ju-Back Fertilizer on Growth of Crop Plants
Lee, Jung-Hoon ; Park, Sung-Min ; Park, Chi-Duck ; Jung, Hyuck-Jun ; Kim, Hyun-Soo ; Yu, Tae-Shick ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1562~1570
DOI : 10.5352/JLS.2007.17.11.1562
This experiment was conducted to develop fertilizer which promotes plant growth as well as suppressing pathogenic fungi. The fertilizer was made from the mixture of Ju-Back (Korean rice wine cake) and indigenous rhizosphere-bacterium. The main ingredients of Ju-Back were investigated as 6.04% total nitrogen, 42.59% total carbohydrate, 1.01% available phosphate, 73.42% organic matter, 7.72% potassium oxide, 1.35% calcium oxide, 0.53% magnesium oxide. The enzyme activities of Ju-Back were estimated to be 980 units/g for
, 300 units/g for glucoamylase, and 1800 units/g for acid pretense. Indigenous rhizosphere bacteria which produced antifungal agent were isolated from soil, and was selected KMU-13 strain which can antagonize against various plant pathogenic fungi (Botrytis cinerea KACC 40573, Sclerotinia sclerotiorum KACC 41065, Fusairum oxysporum KACC 40052, Pythium aphanidermatum KACC 40156, Phytophthora capsici KACC 40476 and Glomerella cingulata KACC 40299). KMU-13 strain was identified as Bacillus subtilis KMU-13 by biochemical and 16s rDNA analysis. The organic fertilizer was made as prototype which was composed 20% Ju-Back, 70% carrier, 9.7% microorganism cultivated solution, 0.3% trace-element. We also investigated an application of fertilizer using Ju-Back for cultivating lettuce (Lactuca sativar) which were grown in three soil conditions that had chemical fertilizer, barnyard manure, lime power, urea, potassium chloride and superphosphate as a control, the whole quantity (80 kg/10a) of posted fertilizer with the control and the half quantity (40 kg/10a) with the control. The growth characteristics were examined and analysed with several weeks interval from 3 weeks to 8 weeks on head length (cm), head width (cm/head), number of leaf and fresh weight (g/plant). The results are summarized as follows. The head width and fresh weight of lettuce were the highest at posted fertilizer 1 (whole quantity) was applied chemical, organic matter (Ju-Back) and carrier. The head length was the highest at posted fertilizer 2 (whole quantity) was applied Ju-Back only.
Effects of Hanbag Mushroom(Grifola frondosa) on Oxidative Stress in Diabetic Rats
Lee, Soon-Yi ; Lee, Chang-Yun ; Park, Yeong-Chul ; Kim, Jong-Bong ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1571~1575
DOI : 10.5352/JLS.2007.17.11.1571
This research was carried out to investigate the effects of Hambag mushroom on the oxidative stress in diabetic rats, Sprague-Dawley. The diabetic rats induced by streptozotocin were fed with hambag mushroom-powder(G. frondosa) for 6 weeks. For the level of oxidative stress in liver and pancreas tissues, it was studied by measuring LPO (lipid oxide) level as an indicator of lipid peroxidation, XOD(xanthine oxidase) as one of important sources for free radicals and the levels of GSH and GST as anti-oxidant systems. Also, as an indicator of liver damaged by oxidative stress, the activities of serum ALT and AST were measured. It was observed that the levels of ALT, AST, LPO and XOD were higher by about two times in both tissues from diabetic rats than in those from control rats. This indicates that the oxidative stress induced by diabetes caused the tissues damages. However, these levels were decreased in the tissues from rats with hambag mushroom-powder. Futhermore, the activity of GST were higher in both tissues from diabetic rats fed with hambag mushroom-powder than in those from diabetic rats. Thus, it is considered that the hambag mushroom-powder decreases the level of oxidative stress by increasing activity of anti-oxidant system such as GSH and GST. It is suggested that the hambag mushroom-powder can be useful for preventing the tissues damaged by diabetes-induced oxidative stress.
Effect of Ondansetron Alone and Combination of Naltrexone and Ondansetron on Alcohol Intake in C57BL/6 Mice
Kim, Hyeun-Kyeung ; Kim, Sung-Gon ; Kang, Cheol-Joong ; Park, Sang-Ick ; Kim, Won-Ho ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1576~1581
DOI : 10.5352/JLS.2007.17.11.1576
Dopamine reward pathway projecting from ventral tegmental area to nucleus accumbens is well known as playing an important role in alcohol dependence. It is supposed that this dopamine pathway is modulated by
nervous system, and it was reported that ondansetron (OND),
receptor antagonist, reduced drinking amount and increased abstinence rate in alcohol-dependent patients. The purpose of this study is to investigate the effect of combination of OND and naltrexone (NTX), non-specific opioid receptor antagonist, on alcohol intake in C57BL/6 mice. In 40 C57BL/6 mice in the state of alcohol dependence, vehicle, while OND 0.01 mg/kg, or NTX 1.0 mg/kg administrated respectively, or OND 0.01 mg/kg and NTX 1.0 mg/kg administrated simultaneously for ten days, medication effects on 2-hr alcohol, 22-hr water, 24-hr food intake and body weight were studied. When vehicle group was compared with 3 medication groups respectively, using a repeated measure ANOVA, NTX alone and vehicle groups showed a significant medication by time interaction (p
Characterization of Bacterial Cellulose Production by Gluconacetobacter sp. JH232.
Ahn, Yeong-Hee ; Park, Jai-Hyo ; Go, Sang-Hee ; Jun, Hong-Ki ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1582~1586
DOI : 10.5352/JLS.2007.17.11.1582
Previous study (J. of Chem. Technol. Biotechnol. 2004, 79, 79-84) showed that bacterial cellulose (BC) produced by a bacterial strain JH232 has potential as a source for environmentally friendly ion exchange membranes. In this study, strain JH232 was investigated for phylogenetic classified and characterized for BC production. Comparative analysis of 16S rRNA gene revealed that the strain belongs to the genus Gluconacetobacter. Maximum production of BC was observed when JH232 was cultured in CSL medium (pH 5.5) at
as determined by flask experiment. When batch and fed-batch cultures of JH232 were performed in the fermenter experiment to compare BC productivity of the strain, BC productivity of fed-batch culture was 1.56 times higher than that of batch culture.
The Structure and Function of Locus Control Region
Kim, Ae-Ri ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1587~1592
DOI : 10.5352/JLS.2007.17.11.1587
Locus control region (LCR) is a cia-acting element which regulates the transcription of genes in developmental stage and/or tissue-specific pattern. Typically, LCR consists of several DNase I hypersensitive sites (HSs), where the binding motifs for transcriptional activators are present. The binding of activators to the HSs recruits chromatin modifying complexes to the LCR, opening chromatin structure and modifying histones covalently through the locus. LCR forms close physical contact with target gene located at a distance by looping away intervening region. In addition, non-coding RNA is transcribed from LCR toward target genes in continuously acetylated active domain. These structural and functional features of LCR suggest that the LCR plays many roles in chromatin activation and transcriptional regulation.
Intestinal and Mesenteric Lymphosarcoma on Alcohol Liver Disease in the Mice
Yuan, Dong-Wei ; Yang, Hai-Jie ; Hong, Il-Hwa ; Ki, Mi-Ran ; Park, Jin-Kyu ; Goo, Moon-Jung ; Lee, Hye-Rim ; Yoo, Seung-Eun ; Jeong, Kyu-Shik ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1593~1595
DOI : 10.5352/JLS.2007.17.11.1593
In the study of investigate the expression and effects of adrenomedullin (AM) in the tissue injured by ethanol in our laboratory, there were lymphosarcomas involving mesenteric lymph node and intestine discovered in experimental mice. Lymphosarcoma is probably the second most common spontaneous neoplastic disease, the incidence of which is dependent on the strain of mouse. The diagnosis of lymphosarcoma was based on gross and light microscopic studies. Crossly, the excised intestine and mesentery showed soft-to-firm, cream-colored, displayed protuberance into the lumen. The neoplastic cells had mainly infiltrated into the mucosal and mesenteric lymph node, and have round hyperchromatic nuclei and a small amount of cytoplasm. The origin of these tumor cells was determined by immunohistochemistry positive of CD20, CD3 and Vimentin.
Induction of Apoptosis by Bee Venom in A549 Human Lung Epithelial Cancer Cells through Modulation of Bcl-2 and IAP Family and Activation of Caspases
Woo, Hyun-Joo ; Kim, Hyun-Joong ; Hong, Su-Hyun ; Hong, Sang-Hoon ; Choi, Byung-Tae ; Lee, Yong-Tae ; Park, Dong-Il ; Choi, Yung-Hyun ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1596~1600
DOI : 10.5352/JLS.2007.17.11.1596
Bee venom is used to treat inflammatory diseases in Korean traditional medicine and has been known to inhibit proliferation and induce apoptosis in cancer cells. However, the molecular mechanisms involved in bee venom-induced apoptosis are still uncharacterized in human lung cancer cells. In the present study, we investigated the effects of bee venom on the apoptosis of A549 human lung epithelial cancer cells. Treatment of bee venom inhibited the cell viability and induced apoptosis in a concentration-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. Bee venom-induced apoptosis in A549 cells was associated with a marked inhibition of anti-apoptotic Bcl-2 expression without significant changes in the levels of Bax and Bcl-xL. Bee venom treatment also inhibited the levels of IAP family members such as cIAP-1 and cIAP-2 and induced the proteolytic activation of caspase-3 and caspase-9. Although further studies are needed, the present results suggest that apoptotic signals evoked by bee vemon in A549 cancer cells may converge caspases activation through a down-regulation of Bcl-2 rather than an up-regulation of Bax. These findings provide important insights into the possible molecular mechanisms of the anti-cancer activity of bee vemon in human cancer cells.
Secretory Overexpression of β-Agarase in Bacillus subtilis and Antibacterial Activity of Enzymatic Products
Jang, Min-Kyung ; Lee, Ok-Hee ; Yoo, Ki-Hwan ; Lee, Dong-Geun ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 17, issue 11, 2007, Pages 1601~1604
DOI : 10.5352/JLS.2007.17.11.1601
The gene for
of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis DB104, 168 and ISW1214 strains for mass-production. Among 3 host strains, B. subtilis ISW1214 secreted the highest amount of recombinant
with a specific activity of 201 U/mg and 360 mg of protein into culture broth. This was approximately 130-fold higher than the production in E. coli as an expression host. Recombinant enzyme produced neoagarooligosaccharides such as neoagarohexaose, neoagarotetraose, and neoagarobiose from agar. Produced neoagarooligosaccharides showed antibacterial activities against gram-negative E. coli and gram-positive B. subtilis at a concentration of 1.5%. These data suggest that neoagarooligosaccharides could be an useful preservative for food industry.