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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
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Development of a Biocontrol Agent Using Monacrosporium thaumasium to Control a Root Knot Nematode, Meloidogyne incognita
Choi, Ye-Hoon ; Kim, Keun-Ki ; Son, Hong-Joo ; Shin, Hae-Soo ; Park, Hyean-Cheal ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1605~1609
DOI : 10.5352/JLS.2007.17.12.1605
The created microbial pesticide was used to examine its effects using the inside-pot test method. The selected microbial pesticide KBC3017 particle pesticide manufactured by using Monacrosporium thaumasium was used in the farm-house outdoor test to find the optimum consistency and its effects. The more amount used, the better effect it showed. However, the optimum consistency was 2% and the KBC3017 particle pesticide for which the diatomite and raw jade powder were used as an increaser, when used 2% level of the total amount of soil, showed 71% effect on nematode prevention. The root and the stem of crops were better compared to those without any pesticide used.
Phylogenetic Study of Genus Sorbus in Korea by Internal Transcribed Spacer Sequence (ITS)
Huh, Man-Kyu ; Kim, Sea-Hyun ; Park, So-Hye ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1610~1615
DOI : 10.5352/JLS.2007.17.12.1610
Genus Sorbus is a long lived woody species that is primarily distributed throughout Asia and Europe. This species is regarded as very important herbal medicines in Korea and China. Sorbus commixta is primarily distributed throughout Europe. We evaluated a representative sample of the four taxa with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within genus. Aligned nucleotide sequences of the length of ITS1 were nearly constant within genus Sorbus varying from 219 in S. aucuparia to 218 in the rest species. Especially, the 5.8S subunit of all taxa of Sorbus was found to constant of 165 bp nucleotides. However, aligned nucleotide sequences of the length of ITS2 vary from 240 in S. sambucifolia var. pseudogrcilisto 245 in S. aucuparia. Total alignment length is 629 positions, of which 35 are parsimony-informative, 32 variable but parsimony-uninformative, and 552 constant characters. The base furtherance showed the difference to the by a total taxon: an average A and T are 17.7% and G and C are 30.4%, 34.2%, respectively. All the four taxa beginning with conserved base paired triplets emerging from single strand regions (domain I). Noteworthy, in the RNA secondary structure proposed for the three Korean Sorbus taxa RNA transcript ITS2, which shows a remarkedly well-conserved folding (domain II). When compared to the European Sorbus (S. aucuparia) of ITS2. ITS analysis may be useful in germ-plasm classification several taxa of genus Sorbus.
Development of Preservation Prediction Chart for Long Term Storage of Fermented Cucumber
Kim, Jae-Ho ; Breidt, Fred ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1616~1621
DOI : 10.5352/JLS.2007.17.12.1616
Off-flavors and odors in fermented cucumbers result from the growth of undesirable microorganisms during the secondary fermentation. Under laboratory conditions using a sterile fermented cucumber slurry medium, the spoilage fermentations were reproduced. Using this system the salt and pH conditions that allow the spoilage to occur were determined by varying the NaCl concentration and pH of the slurry medium. At pH 3, no spoilage was observed, regardless of the salt concentration, while at pH 3.5, pH 4, and pH 4.5, spoilage occurred in the 0 and 2% NaCl samples. For pH 5.0 samples, spoilage products were seen for all NaCl treatments. Based on these results the Preservation Prediction Chart was developed. The Chart may be used for selection of proper pH value and salt concentration for long term storage of fermented cucumber.
Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Gene for Growth and Meat Quality Traits in the Berkshire Breed
Kim, Sang-Wook ; Jung, Ji-Hye ; Do, Kyung-Tag ; Kim, Kwan-Suk ; Do, Chang-Hee ; Park, Jun-Kyu ; Joo, Young-Kuk ; Kim, Tae-Suk ; Choi, Bong-Hwan ; Kim, Tae-Hun ; Song, Ki-Duk ; Cho, Byung-Wook ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1622~1626
DOI : 10.5352/JLS.2007.17.12.1622
This study was conducted to identify useful single nucleotide polymorphisms (SNPs) and determine their association with economically important traits in pig population. Four candidate gene analyses have identified important chromosomal regions and major genes associcated whit economic traits of the pig. For application of the chromosomal information to the pig industry using DNA technology, SNP markers were developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 4 candidate genes (CSF2, IL4, MYOD, RIP140). PCR restriction fragment length polymorphism (PCR-RFLP) assays were developed for these 4 SNPs and used to genotype Berkshire pig populations in Korea.
Expression of c-Jun N-Terminal Kinase (JNK)-Interacting Protein (JIP) in Cultured Rat Hippocampal Neurons
Moon, Il-Soo ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1627~1633
DOI : 10.5352/JLS.2007.17.12.1627
c-Jun N-terminal kinase (JNK)-interacting protein 1 (JIP1), also known as Islet-brain 1 (IB1), is a scaffold protein that is highly expressed in neurons and pancreatic
. In this study subcellular localization of JIP was investigated in cultured rat hippocampal neurons using an antibody that recognize all variants of JIP1, JIP-2 and JIP-3. The overall expression profile of JIP is punctate throughout soma and dendrites. Statistic analysis showed that
of total JIP immunopuncta overlapped with those of excitatory postsynaptic markers SD-95 and
, respectively. In contrast, only
of JIP clusters overlapped with those of inhibitory postsynaptic markers glycine receptor (GlyR) and gephyrin, respectively. JIP clusters overlapped or juxtaposed with SV2 but not GAD, markers for general and inhibitory nerve terminals, respectively. A substantial fraction
of flotillin immunopuncta, a marker for lipid rafts, clusters overlapped with those of JIP. In addition, JIP was highly expressed in some select ends of dendrites but minimal in axons. These data suggest important roles of JIP in excitatory postsynaptic sites, lipid rafts and dendritic ends.
Synergistic Effect of Methanol Extract from Kalopanax pictus and Ascorbic Acid on Antioxidant, Anticancer and Immunomodulatory Activities
Shon, Mi-Yae ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1634~1640
DOI : 10.5352/JLS.2007.17.12.1634
The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2`-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), nitric oxide (NO) scavenging activities and ferric- reducing/antioxidant power (FRAP) assay against extracts of Kalopanax pictus (KP) were measured. Radical scavenging and antioxidant activities were increased depend on the concentration and the effects were enhanced by ascorbic acid (AA). KP extracts and AA had a good anti-proliferating activity against HepG2 cells by MTT assay and induces cells apoptosis, which was demonstrated by flow cytometric analysis. KP extracts and AA caused the arrest of cell-cycle progression at either G0-G1-phase or G2/M-phase, which might be depending upon the KP extracts concentration. In addition, KP extracts and AA are effective in enhancing immunity and nitric oxide production by RAW 264.7 macrophages cells. KP extracts and AA inhibited tumor cell growth and exerted antioxidant effects as compared to controls. These results demonstrate that simultaneous AA and KP extracts treatment could be useful in preventing the oxidative damage and anti-proliferating HepG2 cells, and are effective in enhancing immunomodulatory and antioxidant activity.
Expression of Yippee-Like 5 (YPEL5) Gene During Activation of Human Peripheral T Lymphocytes by Immobilized Anti-CD3
Jun, Do-Youn ; Park, Hye-Won ; Kim, Young-Ho ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1641~1648
DOI : 10.5352/JLS.2007.17.12.1641
Yippee-like proteins, which have been identified as the homolog of Drosophila yippee protein containing a zinc-finger domain, are known to be highly conserved among eukaryotes. However, their functional roles are still poorly understood. Recently we initiated ordered differential display (ODD)-polymerase chain reaction (PCR) to isolate genes of which expressions are altered following activation of human T cells. On the ODD-PCR image, one PCR-product detected in unstimulated T cells was not detectable at the time when the activated T cells traversed near
boundary following activation by immobilized anti-CD3. Cloning and nucleotide sequence analysis revealed that the PCR-product was yippee-like 5 (YPEL5) gene, which was known as a human homolog of the Drosophila yippee gene. Northern blot analysis confirmed the amount of
kb YPEL5 mRNA expression detectable in unstimulated T cells was sustained until 1.5 hr after activation and then rapidly declined to undetectable level by 5 hr. Ectopic expression of YPEL5 gene in human cervix epitheloid carcinoma HeLa cells caused a significant reduction in cell proliferation to the level of 47% of the control. Expression of GFP-YPEL5 fusion protein in HeLa cells showed its nuclear localization. These results demonstrated that the expression level of human YPEL5 mRNA was negatively regulated in the early stage of T cell activation, and suggested that YPEL5 might exert an inhibitory effect on the cell proliferation as a nuclear protein.
Proteomic Analyses of Chinese Cabbage(Brassica campestris L. pekinensis) Affected by High Temperature Stresses in Highland Cultivation During Summer in Korea
Shin, Pyung-Gyun ; Hong, Sung-Chang ; Chang, An-Cheol ; Kim, Sang-Hyo ; Lee, Ki-Sang ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1649~1653
DOI : 10.5352/JLS.2007.17.12.1649
High temperature stresses have caused growth inhibition and delayed heading in highland cultivation Chinese cabbage during summer in Korea. We have studied high temperature stress responses in the terms of changes of inorganic components and proteins by proteomic analyses. Insufficiencies of nitrogen and phosphorus have affected growth rate and calcium deficiency has caused blunted heading. Proteins extracted from Brassica seedling grown at the altitude of 600m and 900m in the Mount Jilun were extracted and analysed by 2-dimentional polyacrylamide gel electrophoresis. Profiles of protein expression was then analyzed by 2-dimentional gel analyses. Protein spots showing different expression level were picked using the spot handling workstation and subjected to MALDI-TOF MS. Total 48 protein spots were analyzed by MALDI-TOF MS and 30 proteins spots out of 48 were identified by peptide mass fingerprinting analyses. Fourteen proteins were up-regulated in extracts from the altitude of 900m and they were identified as oxygen-evolving proteins, rubisco activase and ATPase etc. Sixteen proteins were up-regulated in extracts from the altitude of 600m and they were identified as glutathione S-transferase(1, 28kD cold induced- and 24 kD auxin-binding proteins) and salt-stress induced protein etc. These stress-induced proteins were related to the mediated protective mechanism against oxidative damage during various stresses. The results indicated that physiological phenomenon in response to high temperature stresses might be resulted by complex and multiple array of responses with drought, heat, oxidative, salt, and cold by high temperature.
Effects of Single Total Body Irradiation (TBI) on the Peripheral Blood of Piglets
Lee, Jae-Hoon ; Kang, Eun-Hee ; Chung, Dai-Jung ; Kim, Dong-Ku ; Kim, Jae-Hwan ; Shin, Hyun-Soo ; Ahn, Jung-Yong ; Park, Jin-Ki ; Chang, Won-Kyong ; Choi, Chi-Bong ; Kim, Hwi-Yool ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1654~1659
DOI : 10.5352/JLS.2007.17.12.1654
We examined total body irradiation (TBI)-induced effects by complete blood count (CBC) and fluorescence-activated cell sorter analysis (FACS) in the piglet following radiation irritation. A CBC included red blood cell count, white blood cell count, and platelet cell count. Four piglets were examined in this study and each piglet was divided by irradiation dose, two piglets with 4 Gy, two with 6 Gy, one with 8 Gy. All piglets showed leukopenia, thrombocytopenia after irradiation. In 6 and 8 Gy group, three piglets showed severe hemostatic disorder and gastrointestinal disorder suchas diarrhea and anorexia, and they died between 10 and 15 days after radiation irritation. In 4 Gy, two piglets showed no clinical sign after radiation injury, but persistent leukopenia was shown in blood examination. We suggest that a single TBI dose less than 6 Gy is adequate for conditioning piglet for bone marrow transplantation.
Effects of Non-ionic Surfactant Tween 80 on the in vitro Gas Production, Dry Matter Digestibility, Enzyme Activity and Microbial Growth Rate by Rumen Mixed Microorganisms
Lee, Shin-Ja ; Kim, Wan-Young ; Moon, Yea-Hwang ; Kim, Hyeon-Shup ; Kim, Kyoung-Hoon ; Ha, Jong-Kyu ; Lee, Sung-Sil ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1660~1668
DOI : 10.5352/JLS.2007.17.12.1660
The non-ionic surfactant (NIS) Tween 80 was evaluated for its ability to influence invitro cumulative gas production, dry matter digestibility, cellulolytic enzyme activities, anaerobic microbial growth rates, and adhesion to substrates by mixed rumen microorganisms on rice straw, alfalfa hay, cellulose filter paper and tall fescue hay. The addition of NIS Tween 80 at a level of 0.05% increased significantly (P<0.05) in vitro DM digestibility, cumulative gas production, microbial growth rate and cellulolytic enzyme activity from all of substrates used in this study. In vitro cumulative gas production from the NIS-treated substrates; rice straw, alfalfa hay, filter paper and tall fescue hay was significantly (P<0.05) improved by 274.8, 235.2, 231.1 and 719.5% compared with the control, when substrates were incubated for 48 hr in vitro. The addition of 0.05% NIS Tween 80 to cultures growing on alfalfa hay resulted in a significant increase in CMCase (38.1%), xylanase (121.4%), Avicelase (not changed) and amylase (38.2%) activities after 36 h incubation. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some kinds of cellulolytic enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our SEM observation showed that NIS Tween. 80 did not influence the microbial adhesion to substrates used in the study. Present data clearly show that improved gas production, DM digestibility and cellulolytic enzyme activity by Tween 80 is not due to increased bacterial adhesion on the substrates.
Histochemical Studies of the Intestinal Mucosubstances in Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma, and Helirolenus dactylopterus
Jeong, Gil-Nam ; Jo, Un-Bock ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1669~1674
DOI : 10.5352/JLS.2007.17.12.1669
The conventional histochemical stains were used to study the properties of mucosubstances of the intestine in four teleostean species, i. e., Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma, and Helicolenus dactylopterus, all belonging to the order Perciformes. The following methods were used; periodic acid Schiff (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0, AB pH at 2.5-PAS, and aldehyde fuchsin (AF) pH at 1.7-AB pH at 2.5 stain. The mucosal folds of intestines show differences in species and regions. Widely distributed in all portions studied, goblet cells situated between cylinderical epithelial cells are round or ovoid in shape. They were most densely distributed in distal intestine. In the middle and. distal intestine of Epinephelus chlorostigma and all regions of intestines of Agramus agramus, Inimicus japonicus, and Helicolenus dactylopterus, the presence of both acidic and neutral mucin was confirmed. The property of acidic mucosubstance was sialomucin. Neutral mucin was only encountered in the proximal intestine of Epinephelus chlorostigma. The amount and property of mucin showed differences in species and regions. In the distal intestine of Inimicus japonicus, the amount of acidic mucin is similar to that of neutral mucin. In all regions of intestine of Agramus agramus, proximal and middle intestine of lnimicus japonicus, middle and distal intestine of Epinephelus chlorostigma, and distal intestine of Helicolenus dactylopterus, acidic mucin occured more frequently than neutral mucin. The proximal and middle intestine of Helicolenus dactylopterus have more neutral mucin than acidic mucin.
Lipid Compositions Changes of Seasoned Pork Prepared with Medicinal Plant Extracts During Storage
Cho, Hee-Sook ; Shin, Jung-Hye ; Lee, Soo-Jung ; Kang, Min-Jung ; Cho, Hyun-So ; Sung, Nak-Ju ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1675~1681
DOI : 10.5352/JLS.2007.17.12.1675
The seasoned porks loins manufactured using with seasoning sauce that were prepared with difference composites of medicinal plant extracts (T-0; medicinal plants extract not added,
; pork treated with mixture of 6 kinds medicinal plant, respectively). And the changes of lipids were investigated during storage at
for 15 days. Content of TBARS in raw pork was
MA mg/kg at beginning storage stage, in seasoned pork group (T-1), TBARS content was
MA mg/kg after storage for 15 days. But TBARS contents were ranged from 0.27 to 0.3MA mg/kg in seasoned pork groups (T-1, T-2 and T-3) and it significantly lower than T-0 group. Unsaturated fatty acid content tended to increase during storage, but decreased after storage 9 days. These tendency was equally appeared in the cholesterol content of
groups. In conclusion, the addition of medicinal plant composites enhanced antioxidant activity of the seasoned pork.
Isolation and Characteristics of Bacteria Showing Biocontrol and Biofertilizing Activities
Jung, Ho-Il ; Kim, Keun-Ki ; Park, Hyean-Cheal ; Lee, Sang-Mong ; Kim, Yong-Gyun ; Kim, Hong-Sung ; Lee, Cnung-Yeol ; Son, Hong-Joo ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1682~1688
DOI : 10.5352/JLS.2007.17.12.1682
To develop multifunctional microbial inoculant, microorganisms with antagonistic activity and biofertilizing activity were screened. Pantoea agglomerans and Bacillus megaterium from our laboratory culture collection, and strain MF12 from soil near poultry farm in Miryang were selected. On the basis of morphological, physiological studies and 16S rDNA sequence analysis, isolate MF12 was identified as the Bacillus pumilis. Three strains were studied for insoluble phosphate solubilization, indole-3-acetic acid (IAA) and siderophore production, ammonification ability, hydrolytic enzyme production and antifungal activity against phytopathogenic fungi. P. agglomerans did not produce any visible clear zone on agar plate containing 0.5%
as a sole phosphorus source. However, this strain could solubilize insoluble phosphate in liquid medium. All strains produced IAA ranged from
depending on culture time and had ammonification ability. Among three strains, only P. agglomerans produced siderophore. P. agglomerans produced pectinase and lipase, B. megaterium produced amylase, protease and lipase while B. pumilis produced protease and lipase. P. agglomerans showed antifungal activities against phytopathogenic fungi, Fusarium oxysporum and Colletotrichum gloeosporioides. B. pumilis showed antifungal activities against Botrytis cinerea, Sclerotinia sclerotiorum and Phythium ultimum.
Study on the Vitamin Contents of Commercial Powdered Infant Formula
Bae, Hae-Jin ; Jun, Mi-Ra ; Kim, Young-Gil ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1689~1694
DOI : 10.5352/JLS.2007.17.12.1689
This study was conducted to investigate both fat-soluble and water-soluble vitamin contents of commercial powdered infant formula for obtaining basic data on infant nutrition. Ten commercial infant formula based on cow`s milk were collected and the contents of fat-soluble vitamins (vitamin A, D, E, K) and water-soluble vitamins (vitamin C, thiamin, riboflavin, niacin,
, pantothenic acid, biotin) were compared with Dietary Reference Intakes for Koreans (KDRIs). The overall vitamin contents in 100 g and in 100 kcal of infant formula satisfied the recommended formula regulation (KDRIs) and Codex. In infant formula during 0-5 monthly age, fat-soluble vitamin A, D, E, K could supply 178.6%, 205.3%, 208.4%, 976.3% of adequate daily vitamin intakes, respectively. Water soluble vitamins, vitamin C, thiamin, riboflavin, niacin,
, pantothenic acid, biotin could supply 173.2%, 237.2%, 269.8%, 295.9%, 431.6%, 165.8%, 1186.3%, 203.8%, 408.3% of adequate daily vitamin intakes, respectively. In infant formula during 6-11 monthly age, all vitamins satisfied their adequate daily intakes as well. Vitamin A, D, E, K supplied 199.2%, 262.3%, 220.5%, 626.46% of adequate daily vitamin intakes. Vitamin C, thiamin, riboflavin, niacin,
, pantothenic acid, biotin could supply 179.5%, 210.2%, 264.7%, 241.5%, 206.0%, 166.9%, 699.5%, 247.0%, 475.0% of adequate intake of KDRIs. From this study, evaluation of vitamin contents of commercial infant formula was established, which could strengthen the basic information on infant nutrition.
Growth Inhibition of Helicobacter pylori by Ingestion of Fermented Soybean Paste
Kim, Hyung-Rak ; Kim, Young-Hyu ; Park, Seong-Chan ; Kim, Mi-Sun ; Baik, Keun-Sik ; Cho, Hyun-Wook ; Seong, Chi-Nam ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1695~1700
DOI : 10.5352/JLS.2007.17.12.1695
The aim of this study was to estimate the ingestion effect of fermented soybean paste on the growth inhibition of Helicobacter pylori. Anti-H. pylori effect of the aqueous extract of soybean paste and cell-free supernatant of the isolates from soybean paste were determined using agar diffusion method. Soybean paste and the isolates inhibited the growth of H. pylori. Effect of soybean paste ingestion was estimated using urea breath test against infected volunteers showing no symptom of gastric disease. When 10 g of soybean paste was ingested 3 times a day for 6 weeks, average value of $P
Effect of Physical, Chemical Properties and of Pelleting Solid Materials on the Germination in Pelleted Carrot Seeds
Kang, Jum-Soon ; Son, Beung-Gu ; Choi, Young-Whan ; Lee, Yong-Jae ; Park, Young-Hoon ; Choi, In-Soo ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1701~1708
DOI : 10.5352/JLS.2007.17.12.1701
Seed pelleting is generally conducted in order to save the labor for sowing and thinning by enabling the precision mechanical planting. In the present study, the influence of physical and chemical properties of pelleting solid materials was investigated on carrot seed germination. Among the pelleting solid materials evaluated, dialite, kaolin, and talc showed low bulk density and high porosity. Bentonite and dialite carried high water holding capacities of 184% and 173%, respectively, while calcium carbonate, calcium oxide, and fly ash showed relatively low water holding capacity. The pH of kaolin (6.8) and dialite (7.4) were close to neutral, while limestone (12.8), calcium oxide (13.0), and bentonite (10.0) were highly basic. High electro-conductivity was shown in limestone and calcium oxide. EDS analysis revealed that the main elemental compositions of talc were Si (71.0%) and Mg (29.0%), and those of calcium carbonate were Ca (66.6%), Si (22.9%), and Mg (10.5%). High granulation capacity was observed from talc and the mixture of talc and calcium carbonate. Seeds pelleted with bentonite showed the highest hardness. The dissolving type of the pellet layer after imbibition was split type in talc, limestone, zeolite, and fly ash, melt type in calcium carbonate and calcium oxide, and swell type in bentonite and vermiculite. The shortest dissolving time of pellet layer was observed from calcium carbonate and kaolin. The germination speed
was delayed as the size of pelleted seeds increased. The optimum size of pelleting was 19 ratio in carrot.
Water Extract of Allium sativum L. Induces Apoptosis in Human Leukemia U937 Cells through Reactive Oxygen Species Generation
Choi, Woo-Young ; Chung, Kyung-Tae ; Yoon, Tae-Kyung ; Choi, Byung-Tae ; Lee, Yong-Tae ; Lee, Won-Ho ; Ryu, Chung-Ho ; Choi, Yung-Hyun ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1709~1716
DOI : 10.5352/JLS.2007.17.12.1709
The health benefits of garlic (Allium sativum L.) are derived from a wide variety of components and from the different ways it is administered. The known health benefits of garlic include cardiovascular protective effects, stimulation of immune function, reduction of blood glucose level, protection against microbial, viral and fungal infections, as well as anticancer effects. In the present study, it was examined the effects of water extract of A. sativum (WEAS) on the growth of cultured human tumor cells in order to investigate its anti-proliferative mechanism. Treatment of WEAS to tumor cells resulted in the growth inhibition, especially in leukemia cells, which was associated with induction of G2/M arrest of the cell cycle and apoptosis. In order to further explore the critical events leading to apoptosis in WEAS-treated U937 human leukemia cells, the following effects of WEAS on components of the mitochondrial apoptotic pathway were examined: generation of reactive oxygen species (ROS), alteration of the mitochondrial membrane potential (MMP), and the expression changes of Bcl-2 and IAP family proteins. The cytotoxic effect of WEAS was mediated by its induction of apoptosis as characterized by the occurrence of DNA ladders, apoptotic bodies and chromosome condensation in U937 cells. The WEAS-induced apoptosis in U937 cells was correlated with the generation of intracellular ROS, collapse of MMP, activation of caspase-3 and down-regulation of anti-apoptotic proteins. The quenching of ROS generation with antioxidant N-acetyl-L-cysteine conferred significant protection against WEAS-elicited ROS generation, caspase-3 activation, G2/M arrest and apoptosis. In conclusion, the present study reveals that the cellular ROS generation plays a pivotal role in the initiation of WEAS-triggered apoptotic death in U937 cells.
The Effects of Exercise Training on Cardiac eNOS, ET-1 mRNA and Skeletal Muscle eNOS Protein Level in SHR
Song, Eun-Young ; Cho, In-Ho ; Cho, Joon-Yong ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1717~1722
DOI : 10.5352/JLS.2007.17.12.1717
In the present study, all of the treadmill exercise-trained SHR expressed clear adaptive changes such as reduced resting heart rate and blood pressures, LPOA, homocysteine Therefore, treadmill exercise was sufficient to induce physiological adaptation in the SHR. Endurance training is known to induce physiological cardiac hypertrophy, while hypertension induces patho logical cardiac hypertrophy that increases cardiomyocyte apoptosis. The pathological adaptation to pressure overload has also been associated with a further increase in the expression of several marker genes including cardiomyocyte ET-1 in the SHR, but not in the exercise-trained SHR. Additionally, there is an increase in the endothelial nitricoxide synthases (eNOS) protein expression of soleus, gastrocnemius, and extensor digitorum longus muscle in the exercise-trained SHR but not in the SHR in the present study. Thus, compared to pathological adaptation to pressure overload, physiological adaptation to exercise training is associated with distinct alterations in cardiac and molecular phenotypes. based on these results, exercise training improves hypertension by cardiovascular regulating genes and hemodynamic parameters.
Effect of Antioxidant Activity and Induction of DNA Damage on Human Gastric Cancer Cell by Rubus coreanus Miquel
Jeon, Sang-Kyung ; Lee, Ji-Won ; Lee, In-Seon ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1723~1728
DOI : 10.5352/JLS.2007.17.12.1723
Rubus coreanus Miquel (RCM), a type of red raspberry, grows wild in Korea and China and its unripe fruit is used as a folk medicine for the treatment of impotence and as a diuretic. RCM was extracted with methanol and then further fractionated it into for different types. In this study, we investigated the antioxidant activity of a RCM extract (ext.) and its fraction (fr.). DPPH free radical scavenging activity assay, total polyphenols contents, total flavonoids contents assay were used to analyze antioxidant activity. The DPPH free radical scavenging activity
and total polyphenols contents
were higher in butanol fraction than in other fr. And total flavonoids contents was higher in ethylacetate fr.
. We applied comet assay to measure the DNA damage in the individual cells and exposed time course at
. Comet assay is a rapid and sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. The butanol fro from RCM significantly induced 54.12%, 57.95% of DNA damage after treated RCM for 8 hr. In conclus
Identification of Brassinosteroid-Related Protein, BAK1 from Nutrition Deficient Tomato Cultivated by Soilless Cultivation System
Shin, Pyung-Gyun ; Chang, An-Cheol ; Hong, Sung-Chang ; Lee, Ki-Sang ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1729~1733
DOI : 10.5352/JLS.2007.17.12.1729
Brassinolide insensitive associated receptor kinase 1(BAK1) is a critical component that play an important roles in signaling of brassinosteroid biosynthesis. Brassinosteroid-deficient and -insensitive mutants showed the characteristic of dwarf symptom. The nutrient deficient tomato showing stunt phenomenon was selected from soiless cultivation system using modified Sonneveld hydroponic solution. Twenty eight protein spots showing different expression levels compared to the control were isolated from extracts of stunted tomato leaves by 2D PAGE analyses. Significantly down-regulated 6 protein spots out of 28 protein spots were analyzed and sequenced by MALDI-TOF mass spectrometry. The protein spot having pI
Change of Biological Activity of Strawberry by Frozen Storage and Extraction Method
Youn, Sun-Joo ; Cho, Jun-Gu ; Choi, Ung-Kyu ; Kwoen, Dae-Jun ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1734~1738
DOI : 10.5352/JLS.2007.17.12.1734
The biological activities of strawberry were investigated during frozen storage. The concentrations of total phenolic content in strawberry juice and water extract were
, respectively. The total flavonoid content in strawberry juice and water extract were
. respectively. Antioxidant activities of strawberry juice and water extract were determined. The DPPH of strawberry juice (94.2%) was higher than the water extract of strawberry (93.5%). ABTS of strawberry juice and water extract were 99.9% and 100.0%, respectively. Xanthine oxidase inhibitory activity and SOD-like activity of strawberry of water extracts were higher than those of strawberry juice. The changes in the antioxidant activity of strawberry was insignificant until 6 months of frozen storage. Therefore, it was expected that frozen storage of strawberry was useful preservation expedient for consistent supply of raw materials.
The Effect of Bellydance on the Blood Components, Deoxypyridinoline, Osteocalcin in Middle Age Woman
Ko, Jeong-Rim ; Lee, Hyun-Mi ; Jeon, Jae-Young ; Baek, Yeong-Ho ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1739~1743
DOI : 10.5352/JLS.2007.17.12.1739
The purpose of this study was to develop a movement program adopted from Bellydance, and to analyze the effects of the Bellydance program on the blood Components and Deoxypyridinoline, Osteocalcin in middle aged women. The Bellydance program was composed of warming-up (10min), main exercise (40min) and cooling-down (10min) parts. The 40 middle-aged women between 40 and 60 year old were divided into two group; the control group (8 women), the Bellydance group (8 women). The Bellydance program was applied three times a 12weeks. The result of this study were as follow. RBC, Hb, WBC, PLT, Osteocalcin was measured in a whole blood. The belly dance group showed WBC was more significantly decreased than control group, but RBC, Hb, PLT were insignificant in other groups. The belly dance group showed Deoxypyridinoline was more significantly increased than control group, but Osteocalcin were insignificant in other groups. Therefore this program can be available in middle aged women.
Effects of Walking and Behavior Modification Program on Body Composition, Physical Fitness and Metabolic Syndrome Related Factors in Obese Girls
Kim, Jong-Won ; Jeon, Jae-Young ; Kim, Tae-Un ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1744~1753
DOI : 10.5352/JLS.2007.17.12.1744
The purpose of this study was to provide obese middle school girls with walking and behavior modification program(WBMP) by investigating effects of the exercise on their body composition(BC), physical fitness(PF) and metabolic syndrome(MS). Analysis result of the difference between before and after the exercise(paired and independent t-test) was acquired by measuring BC, PF and MS for 12 weeks(60min/day, three a week). 24 Volunteers were 14 years over
and 15 years over
. In the body composition, waist circumference(WC), body fat mass(BFM), %fat, arm muscle circumference(AMC), total body water(TBW), physical score(PS) were significant difference between the exercise group(EG) and the control group(CG), also including all factors in PF. The components of metabolic syndrome were significant difference between EG and CG, but HDL-C, systolic & diastolic blood pressure were no difference. The metabolic syndrome factors by separate of waist girth were no difference between EG and CG, but BMI, diastolic blood pressure were significant difference. The prevalence of metabolic syndrome was 4 of 24(16.7%) before intervention, but it dropped from 2 to 1 after intervention in WBMP. The components of metabolic syndrome was distributed a big WC>low HDL-C>high TG>hypertension. The changes of AMC, We, PS, BW, 50m run and BFM effect the factors on BMI(35%), WHR(waist-hip ratio, 69%), HDL-C(42%), fasting blood glucose(65%), HOMA-IR index(34%) and systolic blood pressure(39%) respectively. There were significantly increased in breakfast frequency, walking steps, and meal eating period, but decreased in snacks and eating out in exercise group.
Preparation and Characterization of Lactic Acid Bacteria Encapsuled with Alginate Microsphere
Choi, Chang-Yong ; Kang, Seong-Koo ; Park, Seok-Kyu ; Jang, Mi-Kyeong ; Nah, Jae-Woon ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1754~1759
DOI : 10.5352/JLS.2007.17.12.1754
This study is performed to assess the preparation and characterization of lactic acid bacteria (Sterpoccoccus thermophilus) loaded with alginate microsphere using alginate and chitosan for the efficient delivery of lactic acid bacteria to large intestine. Size and morphology of alginate microsphere were confirmed
with spherical shape by scanning electronic microscope (SEM). Biodegradation study of alginate was investigated at different buffer solutions (pH 1.2 and 7.4). This result showed that alginate microsphere did not degrade at pH 1.2 buffer solution but it`s degradation occurred from first day at pH 7.4 buffer solution. Survivability test of lactic acid bacteria in alginate microsphere showed that it was keeping activity of lactic acid bacteria by chroma meter. Therefore, the introduction of alginate microsphere might be a potential system to efficiently delivery lactic acid to large intestine.
Family of Hsp70 Molecular Chaperones and Their Regulators
Chung, Kyung-Tae ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1760~1765
DOI : 10.5352/JLS.2007.17.12.1760
Proteins are involved in promoting or controlling virtually every event on which our lives depend. Proteins are synthesized in cytosol and in the endoplasmic reticulum where their synthesis machinery are tightly controlled. However, not all of newly synthesized proteins are survived and conduct their essential functions to maintain cell`s lives. It was reported that one-third of synthesized proteins are rapidly destroyed by proteasome under the most physiological conditions. full-length translated proteins, which survived, must undergo proper folding and assemble process. Some proteins are spontaneously folded while others require molecular chaperones and folding enzymes to be properly folded. Molecular chaperones are ubiquitously present within the subcellular organelles and from bacteria to animals and plants. Among those members of Hsp70 family have been extensively studied and their regulators have been discovered in the last decade. Here, a brief overview is presented for functional mechanism of Hsp70 homologues and the roles of their regulators. Since biological function of Hsp70 family other than chaperonic function are expending the review would give basic understanding of partnership between Hsp70 family and their regulators.
Mixed Osteosarcoma with Metastatic Alveolar Carcinomatous Appearance in Canine Mammary Gland Tumor
Goo, Moon-Jung ; Hong, Il-Hwa ; Park, Jin-Kyu ; Yang, Hai-Jie ; Yuan, Dong-Wei ; Ki, Mi-Ran ; Lee, Hye-Rim ; Hong, Kyung-Sook ; Han, Jung-Youn ; Hwang, Ok-Kyung ; Kim, Tae-Hwan ; Do, Sun-Hee ; Jeong, Kyu-Shik ;
Journal of Life Science, volume 17, issue 12, 2007, Pages 1766~1770
DOI : 10.5352/JLS.2007.17.12.1766
We describe here a case of malignant mixed osteogenic tumor of the mammary gland with alveolar carcinomatous appreance. A firm, 2 to 2.5cm (in diameter) mass under the 5th nipple, showing the structure of extraosseous osteogenic sarcoma, was removed from the left 5th mammary gland of 12-year-old female dog. When investigated under the microscope, the osteoid material undergoing mineralization was surrounded by numerous scattered osteoblasts and a few osteoclastic cells throughout the osteoid tumorous stroma. The osteoid lesions were continuous with hypercellular myoepithelial cells of a very immature character with several mitotic figures. In addition, there were also carcinomatous tubules and alveoli, with invading cells into peripheral stroma, surrounded by myoepithelial cells in the mammary gland. In these lesions, emanating cords of tumor cells appear to be continuous with the myoepithelial cell layer of a duct. The presence of all these cell types suggests the existence of a common malignant origin, the stem cell being differentiated into epithelial carcinomatous and mesenchymal sarcomatous chondral and osteogenic tissues.