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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
Selecting the target year
Analysis of Quantitative Trait Loci for Yield Component Traits in Soybean Using Recombinant Inbred Lines
Kim, Hyeun-Kyeung ; Oh, Ki-Won ; Choi, In-Soo ; Kang, Jum-Soon ; Choi, Young-Whan ; Lee, Yong-Jae ; Park, Young-Hoon ; Son, Beung-Gu ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 599~605
DOI : 10.5352/JLS.2007.17.5.599
Seed and pod numbers are the main yield components in soybean. Selection for increased yield potential is main goal of plant breeding. The objective of this study was to identify quantitative trait loci(QTLs) that control pod number per plant, seed number per plant and pod in soybean. The 117
recombinant inbred lines(RILs) developed from a cross of `Keunolkong` and `Shinpaldalkong` were used. Two independent QTLs for pod number per plant were identified from linkage group(LG) F and L. Two QTLs for seed number per plant were located on LG F and L. Seed number per pod was related with three QTLs located on LG D1a, D1b and F. Pod and seed number per plant have two common QTLs on LG F and L.
Biochemical Characterization of a Protease with Fibrinolytic Activity from Maggots of Protaetia brevitarsis
Chang, Jeong-Hyun ; Jo, Ji-Young ; Kim, Yeong-Jin ; Lee, Sun-Yi ; Cho, Hyo-Jin ; You, Sun-Nyoung ; Kim, Kwang-Youn ; Park, Byoung-Keun ; Ahn, Soon-Cheol ; Kwon, Heun-Young ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 606~612
DOI : 10.5352/JLS.2007.17.5.606
Fibrin clots remained in blood vessels can be one of the serious factor caused cardiovascular disease, such as ischemia, infarction and necrosis The development of an antithrombotic and thrombolysis solvent is necessary to prevent and treat these diseases. In this study, the fibirinolytic protease was prepared from the maggots of Protaetia brevitarsis using ammonium sulfate fractionation and desalting column. The optimum pH and temperature for the enzyme activity were pH 9.0 and
, respectively. The enzyme activity was relatively stable at pH 7.0-9.0 and temperature below
. The activity of the enzyme was strongly inhibited by phenylmethanesulfonyl fluoride. And the activity of the enzyme was inhibited by
, but it was not by
ions. In these experimental results, we have speculated that the enzyme derived from maggots of Protaetia hrevitarsis is a serine protease with a strong fibrinolytic activity.
Expression analysis of RBMY1, CDY1, and VCY2 genes in Korean male infertility
Huh, Jae-Won ; Kim, Woo-Young ; Kim, Dae-Soo ; Ha, Hong-Seok ; Park, Nam-Chul ; Choi, Ook-Hwan ; Nam, Ki-Man ; Choi, Jin ; Kim, Heui-Soo ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 613~618
DOI : 10.5352/JLS.2007.17.5.613
Azoospermia factor(AZFa, b, and c) regions have been focused on their involvement in the spermatogenic process by frequent observation of microdeletion in male infertility. Among the azoospermia factors, RBMY1, CDY1, and VCY2 genes are strongly associated with the male germinal cell differentiation and development in testis. Using RT-PCR approach, expression patterns of RBMY1, CDY1, and VCY2 genes are examined in testicular biopsy specimens from 42 Korean azoospermic patients. No expression of RBMY1, CDY1, and VCY2 genes appeared as 34%, 66%, and 27% of the male infertility, respectively. Patients who had no expression of RBMY1 and VCY2 genes also showed negative expression of the CDY1 gene in their testis tissues. All Sertoli cell-only syndrome patients showed no expression of the CDY1 gene. Taken together, the CDY1 gene expression seems to be necessary factor to complete spermatogenesis in Korean population.
Comarison of Major Constituents in Acanthopanax Taxa and Variety Cheongsong in Korea by GC-MS
Cho, Kyung-Soon ; Ku, Pyung-Tae ; Huh, Man-Kyu ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 619~624
DOI : 10.5352/JLS.2007.17.5.619
Species of genus Acanthopanax(Araliaceae) are long-lived trees primarily distributed throughout East Asia. These species are regarded as medically and ecologically important in Korea. A variety of Cheongsong in Korea is one of these cultivated varieties, however this variety is much longer(>100 years) than those of other cultivated groups. The components of variety of Acanthopanax in Cheongsong were analyzed for the first time and were compared to those of all Acanthopanax taxa in Korea. Nineteen components were specific to variety in Cheongsong. The main components of this variety were
-caryophyllene, hexadecanoic acid and ethyl stearate. Although some components are differ from each other, variety Cheongsong was similar to A. senticosus at phonetic topology with content of the chemicals, In addition, six species of genus Acanthopanax were investigated to compare the major chemical components by GC-MS.
Purification and characterization of the extracellular alginate lyase from Streptomyces sp. MET 0515
Kim, Hyun-Kyoung ; Lee, Jae-Chang ; Kang, Nam-Hyun ; Kim, Song-Hee ; Kim, Jong-Guk ; Chung, Ki-Chul ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 625~633
DOI : 10.5352/JLS.2007.17.5.625
We isolated a new extracellular alginate lyase-producing microorganism, which displayed alginate-depolymerizing activity in plate assays, from coastal soils in Wando, Jeollanam-do, Korea. This alginate-depolymerizing bacterium belonged to the genus Streptomyces and it was named Streptomyces sp. MET 0515. An extracellular alginate lyase(ALY1) secreted by Streptomyces sp. MET 0515, was purified to homogeneity by a combination of acetone precipitation, anion-exchange chromatography (Q-Sepharose and DEAE-Sepharose) and Sephacryl S-200 HR gel filtration chromatography. Its molecular mass was 26 kDa as determined by SDS-PACE analysis. The enzyme had an optimal temperature of
for its activity, and was most active at pH 7.5. The thermal and pH stability were
, and pH 6.0-9.0, respectively. The enzyme activity was stimulated by 1mM
, and inhibited by 1mM
, 1mM EDTA and 1mM
. Preliminary analysis of substrate specificity showed that this alginate lyase had activity on both poly-alpha 1,4-L-guluronate and poly-beta 1,4-D-mannuronate in the alginate molecule.
Effect of n-3 fatty acid deficiency on fatty acid compositions of nervous system in rats reared by artificial method.
Lim, Sun-Young ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 634~640
DOI : 10.5352/JLS.2007.17.5.634
Our previous study suggested that n-3 fatty acid deficiency was associated with significantly reduced spatial learning as assessed by Morris water maze test. Here we investigated an effect of n-3 fatty acid deficiency on rat brain, retina and serum fatty acyl compositions at 15 wks age using a first generational artificial rearing technique. Newborn Rat pups were separated on day 2 and assigned to two artificial rearing groups or a dam-reared control group. Pups were hand fed artificial milk via custom-designed nursing bottles containing either 0.02%(n-3 Deficient) or 3.1% (n-3 Adequate) of total fatty acids as a-linolenic acid(LNA). At day 21, rats were weaned to either n-3 deficient or n-3 adequate pelleted diets and fatty acid compositions of brain, retina and liver were analyzed at 15 wks age. Brain docosahexaenoic acid(DHA) was lower(58% and 61%, P<0.05) in n-3 deficient in comparison to n-3 adequate and dam-reared groups, receptively, while brain docosapentaenoic acid(DPAn-6) was increased in the n-3 deficient group. In retina and serum fatty acid compositions, the decreased precentage of DHA and increased precentage of DPAn-6 were observed. These results suggested that artificial rearing method can be used to produce n-3 fatty acid deficiency in the first generation and that adequate brain DHA levels are required for optimal brain function.
Isolation and characterization of ethyl methane sulfonate(EMS) Arabidopsis mutants capable of germination under saline conditions.
Chung, Moon-Soo ; Chung, Jung-Seong ; Kim, Cheol-Soo ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 641~645
DOI : 10.5352/JLS.2007.17.5.641
We conducted a seed germination screening under saline conditions to identify salt tolerance(sto) mutants with ethyl methane sulfonate(EMS) mutagenesis seed pool. During the screening, we identified three mutant lines that seemed to confer elevated salt tolerance in high concentrations of NaCl. At 175 mM NaCl, germination rate of sto42-14 mutant(one of the EMS salt tolerance mutants) was 7-fold higher than that of wild-type plants. Interestingly, sto42-14 mutant exhibited insensitivity to high glucose concentration and growth inhibition to gibberellin. Our results suggest that sto42-14 is involved in salt stress tolerance as well as in glucose and gibberellin response in Arabidopsis.
Influence of Nitrate Against Effect of Cadmium on Growth and Rubisco in Seedling of Tobacco.
Roh, Kwang-Soo ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 646~651
DOI : 10.5352/JLS.2007.17.5.646
This investigation was performed to study the influence of Cd and nitrate on growth, and chlorophyll and photosynthetic enzymes in seedling of tobacco. Growth inhibition by Cd was not recovered by nitrate. Chlorophyll levels were reduced by Cd. The combination of Cd and low concentration of nitrate decreased the chlorophyll content compared to that in plants exposed only to Cd. Activity and content of rubisco at Cd treatment was significantly lesser than in plants receiving no treatment, These data suggest that rubisco activity was associated with an amount of rubisco protein, and that the activation and synthesis of rubisco is inhibited by Cd. Both the activity and content of rubisco decreased by Cd were more decreased by nitrate. A similar change pattern was also observed in activity and content of rubisco activase. These results suggest that Cd- and nitrate-induced changes of rubisco could be correlated with rubisco activase, and that nitrate was concerned in not only the activation and synthesis of rubisco directly, but also rubisco activase leading to a large change in rubisco.
Evaluation for the Antimicrobial, Antioxidant and Antithrombosis Activity of Natural Spices for Fresh-cut yam.
Ryu, Hee-Young ; Bae, Kyung-Hwa ; Kim, Eun-Joo ; Park, Sang-Jo ; Lee, Bong-Ho ; Sohn, Ho-Yong ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 652~657
DOI : 10.5352/JLS.2007.17.5.652
To make functional fresh-cut yam using natural spices, the water or the methanol extract was prepared from different kinds of natural spices including ginger, wasabi, black pepper, mustard, cinnamon, chinese pepper, garlic, thyme, rosemary, chamomile, and peppermint. The extraction yields were varied as spice sources and extraction solvents used. Among the water extracts, peppermint extract showed strong antithrombosis activity with 26-folds extended thrombin time, and the
of the extracts from cinnamon and rosemary were
in DPPH scavenging assay, respectively. Whereas, the antimicrobial activity of all water extracts was ignorable. Among the methanol extracts, the extracts from thyme, ginger, and cinnamon showed antimicrobial activity against yam putrefactive bacteria. Strong antioxidative activity was found in the extracts from cinnamon and thyme. Based on these results, the functional fresh-cut yam was prepared using the water extract from peppermint or cinnamon. The antioxidative and antithrombin activities in the fresh-cut yam were maintained at
for 24 hr. Our results suggest that the natural spices could be applied to production of functional fresh-cut yam.
Characterization of a δ-endotoxin produced by Bacillus thuringiensis BT-1, BT-2.
Kim, Young-Min ; Choi, Hong-Seo ; Chung, Kun-Sub ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 658~663
DOI : 10.5352/JLS.2007.17.5.658
Bacillus thuringiensis is a well-known species of entomophathogenic bacteria that is widely used as a biopesticide against many insect pests. It produces parasporal crystals (
-endotoxin) and endospores during sporulation. In this report, the
-endotoxin produced by Bacillus thuringiensis BT-1 and BT-2 were characterized by Scanning Electron Microscope(SEM), Transmission Electron Microscope(TEM), SDS-PACE, and solubilization activity by alkaline solution. BT-1, BT-2 were cultured in the GBY medium, and the
-endotoxin of them was purified with discontinuous sucrose density gradient centrifugation. Their
-endotoxin was observed by SEM and TEM. Morphologically, the
-endotoxin of BT-1 was a square and flat type, whose size was
, and the
-endotoxin of the BT-2 was spherical form whose size was
determined by SEM and TEM. The
-endotoxin of the BT-1 was composed of 28 kDa and 21 kDa, however, it of the BT-2 was composed of 50 kDa, 35 kDa, and 22 kDa bands determined by SDS-PACE. The purified crystals of BT-1 and BT-2 were dissolved gradually in alkaline solution as time goes by, and it was perfectly dissolved after 3 hours. It is supposed that the
-endotoxin of crystal was converted to a state of activation in the course of time in the intestines of insect.
Radioprotective Effects of Propolis on the Mouse Testis Exposed to X-ray.
Ji, Tae-Jung ; Kim, Jong-Sik ; Jeong, Hyung-Jin ; Seo, Eul-Won ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 664~670
DOI : 10.5352/JLS.2007.17.5.664
The propolis is natural product produced by honeybees and is known to have many biologically useful properties such as anti-microbial, anti-oxidative and anti-tumorigenic activity. However, its radio-protective property has not been well studied. To investigate radio-protective effect of propolis on mouse testis, mice were supplemented with propolis after 5 Gy irradiation. The histological changes of testis were detected by TEM. The results indicate that propolis may protect tissue deformation which is induced by 5 Gy of ionizing radiation. Furthermore, to elucidate the potential molecular mechanisms involved in radio-protective property of propolis, we performed microarray experiments using oligo DNA microarray. We found 65 up-regulated genes and 224 down-regulated genes, whose expression levels were affected more than 2-fold by propolis treatment in mice irradiated at 5 Gy. We confirmed microarray data with reverse transcription-PCR using gene specific primers. The results of RT-PCR are highly correlated with those of microarray. These results may help understanding molecular mechanisms of radioprotective effects by propolis in mouse model.
Effects of Water Extract from fermented Chaga Mushroom(Inonotus obliquus) on the Proliferation of Human Cancer Cell Lines.
Cha, Jae-Young ; Park, Sang-Hyun ; Heo, Jin-Sun ; Cho, Young-Su ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 671~677
DOI : 10.5352/JLS.2007.17.5.671
This study was performed to investigate the effect of the water-extract from non-fermented or fermented Chaga mushrooms (Inonotus obliquus) on the proliferation and apoptosis of the NIH3T3 mouse normal fibroblast cells and various human cancer cell lines including HCT-15 human colon carcinoma, AGS human gastric carcinoma, MCF-7 human breast adenocarcinoma, Hep3B human hepatocellular carcinoma and HeLa human cervical carcinoma using MTT(3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide) assay and DNA fragmentation. In an anti-cancer test using various human cancer cells, fermented Chaga mushroom extract showed higher antiproliferating effect than that of non-fermented Chaga mushroom extract. Mouse normal NIH3T3 cells were exhibited 80% above survival under fermented or non-fermented Chngn mushroom extract of various concentrations(0, 0.5 and 1 mg/ml). Fermented Chaga mushroom extract significantly inhibited cell growth on HCT-15 cells in a dose-dependent manner. HCT-15 cells treated with non-fermented or fermented Chaga mushrooms extract produced a distinct oligonucleosomal ladder with different sizes of DNA fragments, a typical characteristic of cells undergoing apoptosis. These results suggest that fermented Chaga mushroom extract suppresses growth of HCT-15 human colon carcinoma cells through apoptosis.
Surface maker and gene expression of human adipose stromal cells growing under human serum.
Jun, Eun-Sook ; Cho, Hyun-Hwa ; Joo, Hye-Joon ; Kim, Hoe-Kyu ; Bae, Yong-Chan ; Jung, Jin-Sup ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 678~686
DOI : 10.5352/JLS.2007.17.5.678
Human mesenchymal stem cells(hMSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum(FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. Previously, we have shown that hADSC can be cultured in human serum(HS) during their isolation and expansion, and that they maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34 cells mobilized from bone marrow in NOD/SCID mice. In this study we determined whether hADSC grown in HS maintain surface markers expression similar with cells grown in FBS during culture expansion and compared gene expression profile by Affymetrix microarray. Flow cytometry analysis showed that HLA-DR, CD117, CD29 and CD44 expression in HS-cultured hADSC during culture expansion were similar with that in FBS-cultured cells. However, the gene expression profile in HS-cultured hADSC was significantly different from that in FBS-cultured cells. Therefore, these data indicated that HS-cultured hADSC should be used in vivo animal study of hADSC transplantation for direct extrapolation of preclinical data into clinical application.
Effects of glucose on metabolism and Insulin-like growth factor binding-3 expression in human fibroblasts.
Ryu, Hye-Young ; Hwang, Hye-Jung ; Kim, In-Hye ; Ryu, Hong-Soo ; Nam, Taek-Jeong ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 687~693
DOI : 10.5352/JLS.2007.17.5.687
Insulin-like growth factor-I(IGF-I) has significant insulin-like anabolic effects which include the stimulation of glucose and amino acid uptake, as well as protein and glycogen synthesis. IGFs exist in serum and other biological fluids as complexes bound to a family of structurally related insulin-like growth factor binding proteins(IGFBPs). Six human IGFBPs can modulate the effects of IGFs on target tissues by several mechanisms, including altering the serum`s half-life and the transcapillary transport of IGFs, as well as changing the availability of IGFs to specific cell surface receptors. Human fibroblasts secrete IGFBPs that can modify IGF-I action. Previous to our study using either Northern blotting, and Western blotting have shown that fibroblasts express mRNA IGFBP-3, -4, and -5, and synthesize these proteins. In addition, fibroblast cell lysates revealed that the IGFBP-3 was most abundant. For these reasons, we undertook to gain further insight into the effects of high and low glucose incubation condition on metabolism and IGFBP-3 expression. In results of metabolites and IGFBP-3 expression in GM10 cells cultivated with various glucose concentration, the consumption of glucose and accumulation of triglyceride were increased in condition of high glucose, and total protein level was decreased. in the course of time. After 5 days incubation, levels of free amino acid in medium containing glucose of high concentration glucose were higher than in conditions of low glucose. Although the levels of IGFBP-3 protein and mRNA levels were increased in low glucose, and IGFBP-3 was not affected by any pretense. Taken together, we suggest that the study of growth factors, like IGFs, might be a possible model of diabetes militus in cell, although the results in cell models were not in accord with in vivo.
Effects of Berberine on Lymphocyte Proliferation and GM-CSF Production in Mice.
Kim, Eun-Young ; Rho, Min-Hee ; Chung, Yang-Sook ; Kim, Hyoung-Su ; Kim, Kwang-Hyuk ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 694~698
DOI : 10.5352/JLS.2007.17.5.694
Berberine, an alkaloid initially isolated from chinese herbal medicine, has antibiotic activities against a variety of organisms including bacteria, viruses, fungi, protozoans, and chlamydia. Furthermore, berberine has shown a number of beneficial effects, including anti-tumor, anti-inflammation, and vasodilatory effects. In this work we have investigated the effects of berberine on lymphocyte proliferation and GM-CSF production in mice. Mouse splenocytes were incubated with berberine and concanavalin A(Con A) to observe the effects on cell proliferation. The culture supernatants of splenocytes exposed to berberine, berberine plus LPS, and berberine plus Con A were harvested to assay GM-CSF. The cell proliferation of nice splenocytes exposed to berberine only(
) was increased significantly more than PBS(control) group. But the Con A-induced cell growth was inhibited by berberine. The GM-CSF production from mice splenocyte culture exposed to berberine only was increased in comparison with PBS(control) group, but the production of it with LPS or Con A was inhibited by berbeline. The present findings may explain lympocyte proliferating and regulating effects of berberine.
Genetic Relationship and Characteristics Using Microsatellite DNA Loci in Horse Breeds.
Cho, Gil-Jae ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 699~705
DOI : 10.5352/JLS.2007.17.5.699
The present study was conducted to investigate the genetic characteristic and to establish the parentage verification system of the Korean native horse(KNH). A total number of 192 horses from six horse breeds including the KNH were genotyped using 17 microsatellite loci. This method consisted of multiplexing PCR procedure. The number of alleles per locus varied from 5 to 10 with a mean value of 7.35 in KNH. The expected heterozygosity and observed heterozygosity were ranged from 0.387 to 0.841(mean 0.702) and from 0.429 to 0.905(mean 0.703), respectively. The total exclusion probability of 17 microsatellite loci was 0.9999. Of the 17 markers, AHT4, AHT5, CA425, HMS2, HMS3, HTG10, LEX3 and VHL20 marker have relatively high PIC value(>0.7). This study found that there were specific alleles, P allele at AHT5, Q allele and R allele at ASB23, H allele at CA425, S allele at HMS3, J allele at HTG10 and J allele at LEX3 marker in KNH when compared with other horse populations. Also, the results showed two distinct clusters: the Korean native horse cluster(Korean native horse, Mongolian horse), and the European cluster(Jeju racing horse, Thoroughbred horse). These results present basic information for detecting the genetic markers of the KNH, and has high potential for parentage verification and individual identification of the KNH.
Upregulation of heme oxygenase-1 by Scutellaria baicalensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells.
Lee, Won-Chol ; Kim, Wan-Sik ; Shin, Gil-Jo ; Moon, Il-Soo ; Jung, Seung-Hyun ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 706~713
DOI : 10.5352/JLS.2007.17.5.706
Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of heme oxygenase-1(HO-1), which has important biological roles in regulating mitochondrial heme protein turnover and in protecting against conditions such as hypoxia, neurodegenerative diseases, or sepsis. Rat cerebrocortical day In vitro(DIV)12 cells were grown in neurobasal medium. On DIV12 cells were treated with SB(
) and given a hypoxic shock (
) on DIV14. In situ hybridization results revealed that SB upregulated HO-1 mRNA in neuronal dendrites in both normoxia and hypoxia(38.5% and 59.2%, respectively). At the protein level, SB upregulated HO-1 in the neuronal soma in both normoxia and hypoxia(22.4% and 15.7%, respectively). Interestingly, most significant increase was associated with astrocytes, which increased HO-1 protein by 77.5% compared to SB-untreated culture. These results indicate that SB upregulates both neuronal and glial HO-1 expression, which contributes to the neuroprotection efficacy in hypoxia).
A Study On the Application Methods of a Support Vector Machine for Gene Promoter Prediction.
Kim, Ki-Bong ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 714~718
DOI : 10.5352/JLS.2007.17.5.714
The high-throughput sequencing of a lot of genomes has resulted in the relatively rapid accumulation of an enormous amount of genomic sequence data. In this context, the problem posed by the detection of promoters in genomic DNA sequences via computational methods has attracted considerable attention in recent years since exact promoter prediction can give a clue to the elucidation of overall genetic networks. In this study, applications of support vector machine(SVM) to promoter prediction are explored to show a right approaches to discriminate between promoter and non-promoter regions by means of SVM. The results of various experiments show that encoding method, encoding region and learning data constitution can play an important role in the performance of SVM.
Construction of the Transgenic Drosophila melanogaster Expressing a Human Megsin Gene.
Goo, Tae-Won ; Kwon, Ki-Sang ; Kwon, O-Yu ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 719~722
DOI : 10.5352/JLS.2007.17.5.719
IgA nephropathy(IgAN) is considered to be a multifactorial disease with genetic and environmental factors contributing to its pathogenesis. The genes involved in susceptibility and progression of the disease have not yet been clearly elucidated. Megsin is an important candidate gene, predominantly expressed in glomerular mesangium and upregulated in IgAN. To understand biological function of megsin, in this work we have produced transgenic D. melanogaster fly over-expressing human megsin(actin-gal4>UAS-Megsin fly). Introduced human megsin was confirmed by RT-PCR and Western blotting, respectively. Its phenotype is melanin deficiency-abdomen and the megsin gene is stably transferred to the next generations.
Mechanism of Metronidazole Resistance Regulated by the fdxA Gene in Helicobacter pylori.
Nam, Won-Hee ; Lee, Sun-Mi ; Kim, Eun-Sil ; Kim, Jin-Ho ; Jeong, Jin-Yong ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 723~727
DOI : 10.5352/JLS.2007.17.5.723
Resistance to metronidazole in Helicobacter pylori results from inactivation of rdxA and frxA, the chromosomal genes for a nitroreductase that normally converts metronidazole from prodrug to bactericidal agent. Two types of metronidazole susceptible strains had been found distinguishable by their apparent levels of frxA expression. Most common in the populations we had studied were strains that required only rdxA inactivation to become resistant to moderate levels of metronidazole(type I strains). The second strain type required inactivation of both frxA and rdxA to become resistance to metronidazole(type II strains): this was linked to a relatively high level of frxA gene transcription in the type II strains. The fdxA gene regulated fdxA as well as rdxA gene. Thus, to study the function of fdxA as a regulatory gene we constructed a null mutant of fdxA in H. pylori genome and identified over-and under-expressed proteins by fdxA using two-dimensional(2-D) electrophoresis and MALDI-TOP-MS. There were four over-expressed proteins in fdxA mutant; nifU-like protein(HP0221), frxA(HP0642), nonheme ferritin(HP0653), and hypothetical protein(HP0902). Three under-expressed proteins were also identified in fdxA mutant, including 5`-methylthioadenosine/S-adenosylhomocysteine nucleosidase (HP0089), (3R)-hydroxymyristoyl ACP dehydratase(HP1376), and thioredoxin(HP1458).
Effects of pH Adjustment on Characteristics of Surimi Using Pork Leg and Chicken Breast.
Jin, Sang-Keun ; Kim, Il-Suk ; Yang, Han-Sul ; Park, Gu-Boo ; Choi, Yeung-Joon ; Shin, Taek-Soon ; Kim, Byeong-Gyun ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 728~734
DOI : 10.5352/JLS.2007.17.5.728
In this study, we prepared surimi from pork leg and chicken breast by pH adjustments of 3.0 and 11.0. The content of crude protein, yield, water-holding capacity, redness, yellowness, myoglobin(Mb) and metmyoglobin(metMb) were significantly higher in the surimi manufactured from pork leg at adjustment pH 3.0 compared to the other surimi samples; whereas whiteness, myofibrillar protein, breaking force, deformation and gel strength were lower than other samples(P<0.05). The textural attributes were significantly higher in the surimi manufactured from pork leg at adjustment pH 11.0 compared to the other surimi samples; whereas Mb, metMb, cooking loss, breaking force, deformation and gel strength were lower than other samples(P<0.05). Again, the content of crude protein, yield, pH, breaking force, deformation, gel strength and lightness were significantly higher in the surimi manufactured from chicken breast at adjustment pH 3.0 compared to the other surimi samples; whereas myofibrillar protein, redness and metMb were higher than other samples(P<0.05). The content of myofibrillar protein, deformation, lightness and cohesiveness were significantly higher in the suriml manufactured from chicken breast at adjustment pH 11.0 compared to the other surimi samples; whereas Mb, cooking loss, yield and breaking force were higher than other samples(P<0.05). The chicken breast surimi had superior color and gel characteristics than manufactured from pork leg, and adjustment pH 11.0 had superior whiteness and cohesiveness than the pH 3.0 adjusted sample, however, there were no significant differences in sensory attributes among the surimi samples.
Aerobic Composting Process of Garbage using Thermoacidophilic Bacillus sp. SJ-15.
Kim, Choon-Hee ; Nam, Soo-Wan ; Choi, Woo-Bong ; Lee, Jong-Hwan ; Kang, Byoung-Won ; Kim, Hweh-Su ; Jeon, Sung-Jong ;
Journal of Life Science, volume 17, issue 5, 2007, Pages 735~739
DOI : 10.5352/JLS.2007.17.5.735
A thermoacidophilic bacterium was isolated from the compost and designated as Bacillus sp. SJ-15 by physiological and biochemical characteristics. The optimum temperature and pH for growth were at
and pH 5.0, respectively. The strain SJ-15 was adapted in process of accelerated high-temperature composting of garbage. The highest viable cell count of composting process reached to
in 16 hours. After running times of 100 days, the composting process showed a reduction rate of approximately 88%, and the concentrations of components were sufficiently high or low to satisfied the standard of organic compost except for salinity.