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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
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Genetic Diversity and Population Structure of Potentilla freyniana in Korea
Huh, Man-Kyu ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 877~881
DOI : 10.5352/JLS.2007.17.7.877
The genetic diversity and population structure of Potentilla freyniana in Korea were determined using genetic variations at 19 allozyme loci. Thirteen of the 19 loci (68.4%) showed detectable polymorphism. Genetic diversity at the population level was high (
Genetic Diversity and Population Structure of Pseudobagrus fulvidraco in the Nakdong River
Huh, Man-Kyu ; Choi, Joo-Soo ; Heo, Youn-Seong ; Lee, Bok-Kyu ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 882~888
DOI : 10.5352/JLS.2007.17.7.882
Enzyme electrophoresis was used to estimate genetic diversity and population genetic structure of Pseudobagrus fulvidraco in Korea. Nine of the 14 loci (64.3%) showed detectable polymorphism. Genetic diversity at the population and species levels were 0.286 and 0.277, respectively. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a substantial deficit of hetero-zygotes relative to Hardy-Weinberg expectations. This deficit is expected that it is due to a limited effective number of individuals per population. The average
for polymorphic loci was 0.064, indicating that most (93.6%) of the genetic diversity occurred within populations. The indirect estimate of gene flow based on mean
was 3.67. Given limited gene flow is expected to diverge genetically due to drift and reduced populations. Most populations in our study experience annual, severe demo-graphic bottlenecks due to drought and floods.
JSAP1 Interacts with Kinesin Light Chain 1 through Conserved Binding Segments
Kim, Sang-Jin ; Lee, Chul-Hee ; Park, Hye-Young ; Yea, Sung-Su ; Jang, Won-Hee ; Lee, Sang-Kyeong ; Park, Yeong-Hong ; Cha, Ok-Soo ; Moon, Il-Soo ; Seog, Dae-Hyun ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 889~895
DOI : 10.5352/JLS.2007.17.7.889
A conventional kinesin, KIF5/kinesin-I, is composed of two kinesin heavy chains (KHCs) and two kinesin light chains (KLCs) and binds directly to microtubules. KIF5 motor mediates the transport of various membranous organelles, but the mechanism how they recognize and bind to a specific cargo has not yet been completely elucidated. Here, we used the yeast two-hybrid system to identify the neuronal protein(s) that interacts with the tetratricopeptide repeats (TRP) of KLCI and found a specific interaction with JNK/stress-activated protein kinase-associated protein 1 (JSAP1/JIPP3). The yeast two-hybrid assay demonstrated that the TRP 1,2 domain-containing region of KLCI mediated binding to the leucine zipper domain of JSAP1. JSAP1 also bound to the TRP region of lac2 but not to neuronal KIF5A, KIF5C and ubiquitous KIF5B in the yeast two-hybrid assay. In addition, these proteins showed specific interactions in the GST pull-down assay and by co-immunoprecipitation. KLCI and KIF5B interacted with GST-ISAP1 fusion proteins, but not with GST alone. An antibody to JSAPI specifically co-immunoprecipitated KIF5s associated with JSAP1 from mouse brain extracts. These results suggest that JSAP1, as KLC1 receptor, is involved in the KIF5 mediated transport.
Induction of Apoptosis by Vitamin E Succinate in Human Erythroleukemia K562 Cells
Jang, Chang-Deug ; Kim, Jong-Myoung ; An, Won-Geun ; Park, Hye-Ryoun ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 896~904
DOI : 10.5352/JLS.2007.17.7.896
Regulation mechanism of apoptosis has been known to be important for understanding the pathogenesis of a number of human diseases including cancers. The effects of
succinate(vitamin E succinate, VES) on the cell viability, generation of ROS, expression of proteins involved in apoptosis, and growth of human chronic myelogenous leukemia K562 cells were analyzed in this study. VES treatment not only induced the generation of the ROS but also increased the levels of
, COX-2, and
in K562 cells. It modulates the levels of pro-apoptotic proteins such as Bax provoking the apoptosis in K562 cells. The cleavage of PARP into 89 kDa was also increased upon VES treatment in a dosage-dependent manner. Induction of an apoptosis was evident by the increase of sub-Gl peak and cell shrinkage condensed chromatin in K562 cells treated with VES. It also resulted in an inhibition of tumor growth by 50% and prolonged survival of the Iymphoma-induced mice. This potentiation of VES obtained in vitro and in vivo may indicate the feasibility of more effective chemotherapy in chronic myelogenous leukemia.
Adenoviral-Mediated Ref-1 Overexpression Potentiates NO Production in Bradykinin-Stimulated Endothelial Cells
Song, Ju-Dong ; Kim, Kang-Mi ; Lee, Sang-Kwon ; Kim, Jong-Min ; Park, Young-Chul ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 905~909
DOI : 10.5352/JLS.2007.17.7.905
The dual-function protein redox factor-1 (Ref-1) is essential for base excision repair of oxidatively damaged DNA and also governs the activation of many redox-sensitive transcription factors. We examined the role of Ref-1 in regulation of nitric oxide (NO) synthesis employing adenoviral-mediatedoverexpression of Ref-1 in bradykinin-stimulated endothelial cells. Intracellular NO was detected with the NO-sensitive fluorophore DAF-2. Overexpression of Ref-1 potentiates bradykinin-stimulated NO production in endothelial cells. And, cells ifected with AdRef-1 showed higher fluorescence intensity compared with uninfected or AdD1312-infected cells. In parallel with this, over expression of Ref-1 also stimulated endothelial NO synthase (eNOS) enzyme activity, compared with unifected or AdD1312-infected cells, in bradykinin-stimulated cells as well as in unstimulated cells. These results suggest that Ref-1 implicates in endothelium-dependent vasorelaxation resulting from NO production in vascular system.
Synthesis and Reaction of 1,5,3,7-Diazadiphosphocine-1,5-Dicarboxylic Acids
Cho, Seung-Hwan ; Song, Ju-Hyun ; Lee, Do-Hun ; Lee, Yong-Gyun ; Park, Yu-Mi ; Choi, Soon-Kyu ; Hahn, Jung-Tai ; Jung, Dai-Il ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 910~914
DOI : 10.5352/JLS.2007.17.7.910
In order to synthesize new bioactive compounds and contrasting agents, reactions of glycine and glutamic acid as an animo acid with paraformaldehyde and hypophosphorous acid were executed. Products are 3,7-dihydroxy-3,7-dioxoperhydro-1,5,3,7-diazadiphosphocine-1,5-diacetic acid 1 and 3,7-dihydroxy-3,7- dioxoperhydre-1,5,3,7-diazadiphosphocine-1,5-di-(2-glu taric acid) 3. 2-[5-(1,2-Dicarboxyethyl)-3,7-dihydroxy-3,7-dioxo-315.715-[1,5,3,7] diazadiphosphocan-1-yl]-succinic acid 2 by using aspartic acid was not obtained. Esterification of 3,7- dihydroxy-3,7-dioxoperkydro-1,5,3,7-diaza-diphosphocine-1,5-diacetic acid 1 by treatment of methanol, ethanol, and propanol were executed. 3,7-Dihydroxy-3,7-dioxoperhydro-1,5,3,7-diazadiphosphocine-1,5-diacetic acid methyl ester 4, 3.7-dihydroxy-3,7-dioxoperhydro-1,5,3,f-diazadiphosphocine-1.5-diacetic acid ethyl ester 5, and 3,7-dihydroxy-3,7-dioxoperhydro-1,5,3,7-diazadiphosphocine-1,5-diacetic acid propyl ester 6 were respectively synthesized in good yields. Continuously, we will try synthesis of novel compounds and evaluation of biological activity.
Identification of Anti-Microbial Material Originated from Opuntia ficus-indica var. saboten Makino
Kim, Hae-Nam ; Cho, Dae-Won ; Yoon, Ung-Chan ; Jun, Hong-Ki ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 915~922
DOI : 10.5352/JLS.2007.17.7.915
In order to discover physiologically active substance, we investigated a powder obtained by processing of Opuntia ficus-indica var. saboten Makino trunk. The powder was treated by sulfuric acid and then extracted by several solvents such as methanol, methylene chloride, ethanol etc. Among them, the best antimicrobial activity was showed by methylene chloride extract. To identify materials exhibiting physiological activation, the acid hydrolyzed extract was separated by 7 fractions through preparative silica gel TLC. The effective fraction exhibiting the best broad antimicrobial activity was identified, named as MBT-01108. From structural analysis of the products extracted to acid hydrolysis, a compound exhibiting the antimicrobial activities is identified to levulinic acid. Levulinic acid isolated from Opuntia ficus-indica var. saboten Makino may be applicable as a natural preservative of food or cosmetic and for prevention of bacterial diseases, an ingredient of acne, ageing and whitening cosmetics and an antimicrobial agent.
Wnt7b is Upregulated in Macrophages during Thymic Regeneration and Negatively Regulated by RANKL
Kim, Jong-Gab ; Kim, Sung-Min ; Kim, Bong-Seon ; Kim, Jae-Bong ; Yoon, Sik ; Bae, Soo-Kyung ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 923~930
DOI : 10.5352/JLS.2007.17.7.923
Thymus can regenerate to its normal mass within 14 days after acute involution induced by cyclophosphamide (CY) in adult rat. Despite the established role of Wnt pathways in the process of thymus development, they have not yet been associated with the regeneration of adult thymus. The purpose of this study was to investigate whether Wnt7b, which is expressed in developing thymic epithelial cells rather than in thymocytes, is modulated during thymic regeneration in adult rat. Here, we show that Wnt7b expression was up-regulated in the regenerating thymus. Cells immunolabeled for the Wnt7b were identified as macrophages. Furthermore, Wnt7b gene expression was decreased by the treatment of receptor activator of NF-kappaB ligand (RANKL). Taken together, our results demonstrate that Wnt7b gene expression was increased in macrophages during thymic regeneration and negatively regulated by RANKL.
Effect of Codium Fragile Extract on Collagen Content and Collagen Cross-link Formation in Ovariectomized Rats
Park, Mi-Hwa ; Kang, Sung-Rim ; Kim, Mi-Hyang ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 931~936
DOI : 10.5352/JLS.2007.17.7.931
The aim of this study was to evaluate the effects of Codium Fragile(CF) extract on the collagen content and collagen cross-link content of the connective tissues, alkaline phosphatase activity and calcium levels of serum in ovariectomized estrogen-deficient rats. Three groups were surgically ovariectomized. The fourth group was sham operated. Sprague-Dawley female rats were randomly assigned to the following groups : sham-oper-ated rats(Sham), ovariectomized control rats (OVX-CON), ovariectomized rats supplemented with CF at 50 mg/kg body wt and 200 mg/kg body wt, respectively The CF were orally administrated at 1mLa day. The ovariectomy caused a decreasing in collagen content in bone, cartilage, skin and lung tissues. However CF groups, supplementation with Codium Fragile extract, increased in collagen contentin bone and cartilage tissues than OVX-CON group. Fyridinoline content in cartilage collagen was de-creased by ovariectomy but supplementation with the CF extracts was similarly increased to Sham. Alkaline phosphatase activity on serum of CF groups decreased than OVX-CON group. These results suggest that CF supplementation prevents post-menopausal bone loss, thus it may be used possibly to improve the quality of life in menopausal women.
Fish Fauna and Weight-Length Relationships for 9 Fish Species in Andong Reservoir
Han, Seung-Cheol ; Lee, Hak-Yeong ; Seo, Eul-Won ; Shim, Jae-Hun ; Lee, Jong-Eun ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 937~943
DOI : 10.5352/JLS.2007.17.7.937
The fish fauna and weight-length relationship in Andong reservoir were investigated from May 2005 to March 2006. During surveyed period, 2,540 individuals 20 species belonging to 7 families were collected. Dominant species were Hemibarbus labeo(19.6%) and sub-dominant species were Carassius cuvieri (19.2%). The korea endemic species were Hemiculter eigenmanni(7.1%), Squalidus chankaensis tsuchigae (0.1 %), Corroperca herzi (0.1 %) and introduced fishes were Carassius cuvieri (19.21 %) Lepomis macrochirus (4.2%), Micropterus salmoides (0.2%). Dominace, species diversity, species richness, species even-ness were 0.39, 3.16, 2.42, 0.73 respectively. The values of the exponent b in the weight-length relation-ship $W
The Study of Application of Bio-Surfactant Producing Bacteria for Growing Crop in Oil Spilled Soil
Hwang, Cher-Won ; Chang, Hae-Won ; Choe, Yong-Rak ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 944~947
DOI : 10.5352/JLS.2007.17.7.944
Bacillus sp.LPO3 (producing emulsifying substances such as bio-surfactant) was used as a bio-control agent to degrade hydrocarbon (gasoline in oil spilled crop soil). The soil (brought from fertilizer store)was mixed with gasoline-spilled soil (made with Diatomaceous Earth, Sigma.U.S.A). The study was conducted for a period of 13 days, 13 days during which bacterial growth, hydrocarbon degradation and growth parameters of Bacillus sp.LP03 including shoot and root length were studied. We found that the effective of bacterial producing substance might bio-surfactants let the plants survive even more promote the growth of shoot and root length and showed antifungal activity against gray mold. Without the bacteria, they couldn`t grow in oil-spilled soil not even survive. According to the results of the above experiments, we can see with following results, hydrocarbon in gasoline was reduced, day by day, then RNA dot blotting was done and it fit the results we had done. Finally, this Bacteria(producing bio-surfactant) were found to have effective bio-control agent for cropping in oil spilled soil and infected by gray mold.
Effects of AEBSF on the Delay of Spontaneous Apoptosis and the Trans-Differentiation of Human Neutrophils into Dendritic Cells
Park, Hae-Young ; Kwak, Jong-Young ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 948~955
DOI : 10.5352/JLS.2007.17.7.948
Neutrophils play a key role as a first line of defense and are known to acquire the characteristics of dendritic cells (DCs) under the appropriate conditions. The spontaneous apoptosis of neutrophils was delayed by treatment with 4-(2-aminoethyl) benzensulfonylfluoride (AEBSF), a serine protease inhibitor. AEBSF inhibited both caspase-3 and serine protease activities, whereas ZVAD-fmk, a pancaspase inhibitor, inhibited only caspase-3 activity. The life span of neutrophils was prolonged up to 5 days by AEBSF in the presence or absence of granulocyte macrophage colony stimulating factor(CM-CSF). DC surface markers, such as CD80, CD83, and MHC class ll were not expressed on neutrophils treated with AEBSF alone. CM-CSF failed to prolong the survival time of neutrophils up to3 days but increased the expression levels of DC markers on neutrophils in the presence of AEBSF. Expression levels of DC markers were the highest on neutrophils treated with CM-CSF and AEBSF for 3 days. AEBSF and CM-CSF-treated neutrophils stimulated proliferation of T cells in the presence of a superantigen, Staphylococcal enterotoxin B (SEB) but produced
) in the absence of SEB. These results suggest that the inhibition of serine protease activity prolonged the life span of human neutrophils and combined treatment of neukophils with CM-CSF and serine protease inhibitor induced differentiation of neutrophils into DC-like cells.
Effects of Iron Shot Practice Volume Based on Golf Skill Level on the Indices of Muscle Damage and Fatigue in Blood
Suh, Ah-Ram ; Paik, Il-Young ; Suh, Sang-Hoon ; Jin, Hwa-Eun ; Kim, Young-Il ; Cho, Soo-Young ; Kwak, Yi-Sub ; Woo, Jin-Hee ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 956~963
DOI : 10.5352/JLS.2007.17.7.956
The purpose of the present study was to examine the effects of iron shot practice volume based on golf skill level on the indices of muscle damage and fatigue in blood. Twenty four golf players were studied under conditions of different golf skill level (beginners, intermediate, expert) and practice volume (100, 200, 300balls). In this study blood CK and LDH were determined to show the level of muscle damage, and blood phosphorus, lactate, and ammonia were measured to see the level of fatigue. Blood creatinine, and IGF- I generally used to see the rate of protein synthesis were also measured. In summary, these results are interpreted to mean that 1) blood fatigue factors, muscle damages, and creatinine levels increased due to a rise in the practice volume of iron shot, 2) those levels decreased due to rise in the golf skill level, 3) blood fators and creatinine recovered quickly after exercise, however muscle damages did not recover even after 24hr, Therefore, both golf skill lev-el and practice volume should be considered to prevent muscle damage and fatigue during practice iron shot.
Quality and Sensory Score of Ground Pork Meats on the Addition of Pork Fat, Olive Oil and Soybean Oil
Youn, Dong-Hwa ; Park, Kyung-Sook ; Lee, Kyung-Soo ; Park, Hyun-Suk ; Moon, Yoon-Hee ; Yang, Jong-Beom ; Jung, In-Chul ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 964~969
DOI : 10.5352/JLS.2007.17.7.964
This study was carried out to investigate the effects of addition of pork fat, olive oil and soybean oil on the quality and sensory of ground pork meat. The samples consisted of the ground pork meat containing 20% pork fat (GP-P), 20% olive oil (GP-O), and 20% soybean oil (GP-S). The chemical composition, surface color, fatty acid composition, water hold-ing capacity, pH, VBN content and TBARS value were determined for the ground pork meat as the quality characteristics, and the sensory score were also evaluated. The moisture, crude protein, crude fat and crude ash content were not different among the GP-P, GP-O and GP-S. The
(yellowness) of /GP-P were higher than those of the GP-O and GP-S (p<0.05). Palmitic acid was the most abundant among saturated fatty acids, and palmitic acid content of GP-P (24.384%)was higher than that of the GP-O (15.611%) and GP-5 (14.423%). In case of unsaturated fatty acids, oleic acid of GP-P (43.773%) and GP-O (65.040%) were the highest, linoleic. acid for GP-5 (40.762) was the highest. The water holding capacity of GP-P was higher than that of the GP-0 and GP-5, the pH of GP-S was higher than that of the GP-P and GP-O, and the VBN content and TBARS value of GP-P was higher than that of the GP-O and GP-5 (p<0.05). The raw color of GP-0 and GP-S were higher than that of the GP-P (p<0.05), however the raw aroma was not different among the samples. In case of roasted ground pork meat, the aroma was not different among the samples, the taste, texture and palatability or GP-S were the highest among the samples, and the juiciness of GP-O and GP-S were higher than that of the GP-P(p<0.05).
Isolation and Characterization of Lactobacillus brevis AML15 Producing γ-Aminobutyric acid
Shin, Ji-Won ; Kim, Dong-Geol ; Lee, Yong-Woo ; Lee, Hyoung-Seok ; Shin, Kee-Sun ; Choi, Chung-Sig ; Kwon, Gi-Seok ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 970~975
DOI : 10.5352/JLS.2007.17.7.970
For the screening of
acid (CABA)-producing bacteria, 86 bacterial strains which produce GABA were isolated from Kimchi and Salted fisk .Among these, three strains designated AML15, AML45-1, AML72 with relatively high GABA productivity were selecled by thin layer chromatography (TLC). To elucidate the relationship between isolated strains and the genus Lactobacillus, their 16S rDNA sequence were examined. The result of their DNA sequences showed 99% similarity with Lactobacillus brevis ATCC 367. On the basis of the these results, isolated strains were identified as Lactobacillus brevis and designated L. brevis AML15. In order to determine the optimum conditions for GABA production, the isolated strains were cultivated in pyridoxal phosphate (PLP) and monosodium glutami. acid (MSG). Results showed that L. brevis AML15 had the highest CABA productivity with 10,424
concentration in MRS broth containing 5% (w/v) MSG and 10
PLP at pH 5.0. The results imply that L. brevis AML15 has the potential to be developed as a strain for GABA hyper-production.
Effects of Histone Deacetylase Inhibitor, Trichostatin A, on the Differentiation of C2C12 Myoblasts and the Expression of Cell Cycle Regulators
Lee, Won-Jun ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 976~982
DOI : 10.5352/JLS.2007.17.7.976
The purpose of this study was to determine the modulating effects of histone deacetylase inhibitor, trichostatin A, on the differentiation of mouse C2C12 myoblasts. We demonstrated that trichostatin A induced morphological changes of C2C12 myoblasts into smooth muscles and significantly increased the gene expression of smooth muscle markers including smooth muscle
and transgelin. These results were due to the change in the expression level of cell cycle regulators in trichostatin A-treated C2C12 cells. Real-time PCR data revealed that cyclin dependent kinase inhibitor, p21, mRNA expression was significantly increased in trichostatin A-treated C2C12 cells. However, trichostaDn A rapidly decreased cyclin Dl mRNA expression necessary for cell cycle progression in 24hr after treatment. In conclusion, the strong inhibitory effects of trichostatin A on histone deacetylation induced transdifferentiation of C2C12 myoblasts into smooth muscle cells and these results are partly due to the changes in the expression of cell cycle regulators such as p21 and cyclin D1.
Confirmation of Non-Siderophore Antifugal Substance and Cellulase from Bacillus lichemiformis Kll Containing Antagonistic Ability and Plant Growth Promoting Activity
Woo, Sang-Min ; Kim, Sang-Dal ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 983~989
DOI : 10.5352/JLS.2007.17.7.983
Bacillus lichemiformis Kll, a plant growth promoting rhizobacterium was reported as a producer of auxin, siderophore, as well as antifungal cellulase under some culture conditions. In vitro test, B. licheniformis Kll represented excellent antagonistic ability against Fusarium oxyspoum (KACC 40037), and showed broad spectrum against other phytopathogenic fungi. B. licheniformis Kll had cellulolytic activity toward not only carboxymethyl-cellulose (CMC) but also insoluble cellulose, such as fungal cell wall cellulose, filter paper (Whatman No. 1), and Avicel. In addition, we confirmed antifungal substance production by butanol-extract methods. The strain produced optimally the antifungal substance when it was cultivated at pH 9.0, 30
C for 4 days on nutrient medium. The biological control mechanisms of B. lichemiformis Kll were caused by antifungal substance, cellulase and siderophore against phytopathogenic fungi.
The Effect of Sodium Tungstate on the Aldehyde Oxidase and the Growth in the Primary Root of Maize (Zea mays)
Oh, Young-Joo ; Cho, Young-Jun ; Park, Woong-June ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 990~995
DOI : 10.5352/JLS.2007.17.7.990
We tested the effect of sodium tungstate, which disturbs the molybdenum cofactor formation, on the activities of aldehyde oxidase(AO) and the growth of maize(Zea mays) primary roots. As reported in other plants, sodium tungstate inhibited AO also in the maize root concentration-dependently. The inhibitory effect of sodium tungstate was observed only when the inhibitor was applied to the living plants. Application of tungstate to the extracted protein did not show any effect. Western analysis revealed slightly decreased level of AO protein in the presence of tungstate, indicating a positive feedback of gene regulation by the product. We also tested the effects of tungstate on the root growth. The elongation of primary root and the development of lateral roots, which are sensitive to the absolute level of auxin, were decreased in the presence of sodium tungstate. However, the gravitropic curvature of the primary root, which is dependent on the relative amount of auxin at both sides, was unaffected. These data suggested the decrease of auxin biosynthesis by the application of tungstate. However, the level of free IAA was unaffected by tungstate application. We discuss the possible explanations for the observed results.
Analysis of Differentially Expressed Genes by Sulindac Sulfide in Human Colorectal Cells
Shin, Seung-Hwa ; Kim, Jong-Sik ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 996~1001
DOI : 10.5352/JLS.2007.17.7.996
To investigate whether sulindac, sulindac sulfone, and sulindac sulfide could affect cancer cell viabilities, human colorectal HCTl16 cells were treated with 10
of each NSAID. Among treated NSAms, sulindac sulfide dramatically decreased the cell viabilities detected by MTS and the cytotoxic effect showed dose-dependent manner. To understand the molecular mechanism of cell death in response to sulindac sulfide treatment, we performed oligo DNA microarray analysis. We found that 23 genes were up-regulated more than 2 folds, whereas 33 genes were down-regulated more than 2 folds by treatment of 10
sulindac sulfide. Among the up-regulated genes, we selected 3 genes (NAG-1, DDIT3, PCK2) and performed RT-PCR and quantitative real-time PCR to cofirm microarray data. The results of RT-PCR and real-time PCR were highly accorded with those of microarray experiment. As NAG-1 is well-known gene as tumor suppressor, we detected changes of NAG-1 expression by 10
of sulindac, sulindac sulfone, and sulindac sulfide. The results of RT-PCR and quantitacve real-time PCR indicated that sulindac sulfide was the strongest inducer of NAG-1 among treated NSAIDS. This result implies that induction of NAG-1 by sulindac sulfide plays important role in cell death of colorectal cancer. Overall, we speculate that these results may be helpful in understanding the molecular mechanism of the cancer chemoprevention by sulindac sulfide in human colorectal cancer.
Helicobacter pylori and Gastric Carcinogenesis
Myong, You-Ho ; Choi, Yung-Hyun ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 1002~1018
DOI : 10.5352/JLS.2007.17.7.1002
Ever since the World Health Organization classified Helicobacter pylori as a class I carcinogen, a variety of discussions over the actual role of H. pylori infection in gastric carcinogenesis has existed. Although a majority of researches support the positive correlation between H. pylori infection and the development of gastric cancer, many aspects of this association are yet uncertain, and some data even suggest that there may be no correlation between H. pylori infection and gastric carcinogenesis. However, there are proofs indicating these reports underestimated the prevalence of H. pylori infection and therefore, the association of the infection and gastric adenocarcinoma. In this report, I reviewed the epidemiology of H. pylori and gastric cancer, evidence supporting and against the positive correlation of the infection and the disease, and the possible pathological role H. pylori infection may have in gastric carcinogenesis referring particular to published literature. As a conclusion, despite a few reports of a possible negative or no relationship between gastric cancer and H. pylori infection, I was able to find that H. pylori infection did have a pathological role in the development of gastric cancer.
Comparison of Effects between Alteplase and Pamiteplase on MMPs Regulation
Jung, Jae-Chang ; Lee, Sun-Ryung ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 1019~1022
DOI : 10.5352/JLS.2007.17.7.1019
Thrombolytic therapy with tissue plasminogen activator (tPA) can improve the clinical outcome of ischemic stroke patients. However, its clinical application is limited by narrow therapeutic time windows and elevated risks of cerebral hemorrhage and brain injury. In part, these effects of tPA has been related to matrix metalloproteinase-9 (MMP-9) dysregulation. Here, we investigate that the effects of alteplase (tPA with short half-life) and pamiteplase (a modified tPA with long half-life) on the MMP-9 regulation in neurovascualr unit. The total levels of MMP-2 and MMP-9 in neuronal cells are lower than astrocytes. Alteplase (1-10
) induced upregulation of MMP-2 and MMP-9 in rat cortical neurons and astrocytes, respectively. Whereas pamiteplase in a wide range of dose did not affect the MMP-2 and MMP-9 responses in both of cells. These results suggest that pamiteplase with long half-life can be provided as a agent that overcome the side effects of alteplase.
Expression of Ajuba, a Novel LIM Protein, is Regulated by Endorlasmic Reticulum Stress
Park, Sang-Mi ; Kwon, Ki-Sang ; Yun, Eun-Young ; Goo, Tae-Won ; Kwon, O-Yu ;
Journal of Life Science, volume 17, issue 7, 2007, Pages 1023~1025
DOI : 10.5352/JLS.2007.17.7.1023
Ajuba is a number of proteins containing cytosolic LIM domain. Its function may provide a new pathway whereby cell-cell adhesive events are transmitted to the nucleus to regulate cell proliferation and differentiation decisions. Here, Ajuba gene expression was investigated its molecular properties associated with endoplasmic reticulum (ER) stresses (tunicamycin, DTT, A23187 and BFA) which induced remarkable ex-pression of Ajuba mRNA. The mRNA half life of Ajuba was also determined, its half life of Ajuba mRNA in FRTL-5 cells was approximately 2 hr after the initial translation. Although the obvious bioligical function of Ajuba is not clear, on the base of the results, Ajuba gene expression is deeply associated with ER stresses.