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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
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Relation of Poly(ADP-ribose) Polymerase Cleavage and Apoptosis Induced by Paclitaxel in HeLa S
Uterine Cancer Cells
Chang, Jeong-Hyun ; Kim, Kwang-Youn ; Ahn, Soon-Cheol ; Kwon, Heun-Young ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1027~1033
DOI : 10.5352/JLS.2007.17.8.1027
Although paclitaxel induces apoptosis of cancer cells, its exact mechanism of action is not yet known. The present study has been performed to determine whether influence of paclitaxel in HeLa
uterine cancer cells. Three assays were employed in this study: cell cytotoxicity, morphological assessments of apoptotic cells (DAPI staining assay), and western blot analysis. The results indicated that paclitaxel has cytotoxic effects in HeLa
cells. Especially, the
value of paclitaxel was about 1
. And morphological changes (fragmentation) of cells were observed by paclitaxel in HeLa
cells. The flow cytometric analysis of paclitaxel-treated cells indicated a block of G2/M phase. The results that pacli-taxel regulates the cell cycle, especially Sub-
phase. Paclitaxel induces apoptosis of HeLa
cells via PARP-dependent fashion, and this apoptosis is related to disappearance of Bcl-2 proteins.
Detect of Hypericin (HyH) gene in Hypericum erectum in Korea and Comparison of H. perforatum in Europe
Huh, Man-Kyu ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1034~1038
DOI : 10.5352/JLS.2007.17.8.1034
Hypericin (HyH) is a substance which is isolated a medicinal herb, Hypericum perforatum L., commonly known as St. John`s Wort. Hypericum erectum is a long-lived herb that is distributed in Korea. Cloned HyH genes H. erectum of were conformed by sequencing. The cDNA Hyp-1 sequence has 732 bp with an open reading frame of 567. Thus coding for a protein of 152 amino acid residues. A BLAST re-search using the deduced nucleotide sequences in HyH gene produced significant alignments with the H. perforatum. Sequences in HyH gene showed significant homology with Rubus idaeus putative allergen Rub-i-1 mRNA, Protein sequence comparisons revealed significant homology between Hyp-1 and the phenolic oxidative coupling protein hyp-1 of H. perforatum (98%). Additionally, Hyp-1 showed sig-nificant homology with various other classes of allergens, including Pru-av-1 (62%) from Prunus avium and allergen Bet-vl-Sc3 from Betula pendula (60%). Thus, the result of this study may offer an important information to establish an assay system for chemicals of the herbal medicines for H. erectum as well as H. perforatum.
Chemotherapeutic Drug Resistant Cancer Stem-like Cells of Glioma
Kang, Mi-Kyung ; Kang, Soo-Kyung ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1039~1045
DOI : 10.5352/JLS.2007.17.8.1039
Glioblastoma multiforme (GBM) is the most frequently occurring brain cancer. Although the existence of cancer stem cells (CSCs) in GBM has been established, there is little evidence to explain the link between CSCs and chemoresistance. In this study, we investigated that only a few cells of A172 and established GBM2 survived after 1,3-bis(2chloroethyl)-1-nitrosourea (BiCNU) exposures and these sur-vived cells resist the subsequent BiCNU treatment. In addition, these BiCNU-resistant small pop-ulations derived from GBM cells increased the phosphorylations of Erk and Akt and highly expressed CD133 stem cell surface marker. Furthermore, we observed that the BiCNU-resistant cancer cells de-rived from GBM have grown tumors when transplanted into severe combined immuno-deficient (SCID) mouse brain. These results demonstrate that BiCNU-resistant subpopulation cells derived from GBM have cancer stem-like cell properties. Therefore, it may provide provide further evidence that CSCs in GBM have chemotherapeutic drug resistance.
Characterization and Distribution of Transferrin from the Last Larval Haemolymph of Papilio xuthus
Shin, Myung-Ja ; Kim, Kyoung-Keun ; Kim, Jeong-Sook ; Lim, Jae-Hwan ; Seo, Eul-Won ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1046~1052
DOI : 10.5352/JLS.2007.17.8.1046
Transferrin is a molecule carrying iron to store and maintain for iron homeostasis of living organisms. In this study, we have purified transferrin, as an iron-binding protein, from the last larval haemolymph of Papilio xuthus by KBr density gradient ultracentrifugation and gel filtration (superose 6 HR) using fast protein liquid chromatography (FPLC) and transferrin containing iron was identified by Ferene S staining. The purified haemolymph transferrin was shown to have molecular mass of 78 and 80 kDa and amino acid composition of transferrin was rich in aspartic acid, valine, leucine and glutamic acid. With immuno-diffusion assay, we confirmed the existence of the transferrin in the haemo-lymph and fat body by detection of visible and clear positive reaction. From the quantitative comparison by rocket immuno-electrophoresis process, the amount of transferrin were increased in the haemolymph of 3 days after pupation and the whole 5 days after pupation. Here, with biochemical and immunohistochemical analysis, we speculate the relationship of transferrin between the physical characteristics and distribution during metamorphosis of P. xuthus.
Tamoxifen Induces Mitochondrial-dependent Apoptosis via Intracellular Ca
Jang, Eun-Seong ; Kim, Ji-Young ; Kim, Byeong-Gee ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1053~1062
DOI : 10.5352/JLS.2007.17.8.1053
In the present work, we show that tamoxifen(Tam)-induced cytotoxicity is due to the mitochondrial-dependent pathway triggered by the intracellular
increase in MCF-7 human breast cancer cells. Tam induced the intracellular
increase. According to the experimental results with
channel blockers, Tam-induced
uptake seemed to depend on the voltage-sensitive
channel at the early stage, but at later stages the intracellular
increases are more likely due partly to the release of stored
and partly to the capacitative
or other entry pathways. Tam-induced
increase led to the release of cytochrome c from mitochondria into the cytosol and the change of mitochondrial membrane potential. In MCF-7 cells, caspase-7 plays a key role in the downstream of apoptosis because caspase-3 is absent. In the cells treated with Tam, caspase-7 cleavage was increased almost two-fold. There was no marked alteration in the level of anti-apoptotic Bcl-2 protein; however, the cells showed increased expression of pro-apoptotic Bax protein more than two-fold in response to Tam. These results imply that the apoptotic signaling pathway activated by Tam is likely to be mediated via the mitochondrial-dependent pathway.
Mcl-1 is a Binding Partner of hNoxa
Park, Sun-Young ; Kim, Tae-Hyoung ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1063~1067
DOI : 10.5352/JLS.2007.17.8.1063
The Bcl-2 family proteins play critical roles in regulation of apoptosis, and the balanced interaction of pro- and anti-death members is a key factor in determining the cell fate. Noxa, a BH3-only Bcl-2-family member, has been originally identified as a target gene of p53. To understand the mechanism by which human Noxa (hNoxa) regulates the cell death, we screened the hNoxa binding partner using the yeast two hybrid screening and found that anti-death protein Mcl-1 binds to hNoxa. The binding of hNoxa to Mcl-1 was confirmed by immunoprecipitation in human colon cancer cell line HCT 116 cells. Mcl-1 significantly inhibited the hNoxa-induced cell death in HCT 116 cells. During the cell death induced by hNoxa, Mcl-1 protein was degraded. Its degradation was inhibited by z-VAD-fmk, a pancaspase inhibitor, suggesting caspase is responsible for Mcl-1 degradation in response to hNoxa. Together, the results indicate that hNoxa binds to Mcl-1 that is degraded by cas-pases during hNoxa-induced cell death.
Distribution of Parvalbumin-Immunoreactive Retinal Ganglion Cells in the Greater Horseshoe Bat, Rhinolophus ferrumequinum
Jeon, Young-Ki ; Kim, Tae-Jin ; Lee, Eun-Shil ; Joo, Young-Rak ; Jeon, Chang-Jin ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1068~1074
DOI : 10.5352/JLS.2007.17.8.1068
Parvalbumin occurs in various types of cells in the retina. We previously reported parvalbumin distribution in the inner nuclear layer of bat retina. In the present study, we identified the parvalbumin-immunoreactive neurons in the ganglion cell layer of the retina of a bat, Rhinolophus ferrumequinum, and investigated the distribution pattern of the labeled neurons. Parvalbumin immunoreactivity was found in numerous cell bodies in the ganglion cell layer. Quantitative analysis showed that these cells had medium to large-sized somas. The soma diameter of the parvalbumin-immunoreactive cells in the ganglion cell layer ranged from 12.35 to 19.12
Alterations of Human Autonomic Nervous System Activity on Capsaicin Ingestion, and Variants of UCP1 and β
- -adrenergic Receptor Polymorphism
Ko, Ki-Jun ; Shin, Ki-Ok ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1075~1081
DOI : 10.5352/JLS.2007.17.8.1075
We investigated whether 1) capsaicin ingestion (100 mg) enhances autonomic nervous system (ANS) activities associated with thermogenic sympathetic activity as energy metabolic modulator, 2) UCP1 and
-AR variants of each subjects influence with ANS activity. Eight healthy males (24.7
1.8 yr) volunteered for this study. The cardiac autonomic nervous activities evaluated by means of heart rate variability of power spectral analysis and energy metabolism were continuously measured during 5-min rest for total 90-min resting condition with placebo or capsaicin oral administration chosen at random. The results indicated that there were no significant differences in heart rate during rest between both trials. Autonomic nervous activity increased in capsaicin tablet trial, but the difference did not reach the statistical significance. Capsaicin, however, induced significantly lower respiratory gas exchange ratio at Test3 (CAP: 0.80
0.02 vs. 0.85
SE, p<0.05). In conclusion, it may be suggested the capsaicin consumption as a valuable supplement for the treatment of individual with hyperlipidemia and/or obesity by improving lipolysis. Further studies will also be considered genetic variants such as UCP1 and/or
-AR associated with obesity.
Identification of Tumor Antigens in Lung Cancer Patient by SEREX
Min, Young-Ki ; Ha, Jin-Mok ; Son, Young-Ok ; Park, Hae-Rim ; Lee, Min-Ki ; Park, Yeoung-Min ; Kim, Cheol-Min ; Lee, Sang-Yull ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1082~1089
DOI : 10.5352/JLS.2007.17.8.1082
Serological anlysis of recombinant cDNA expression libraries (SEREX) has led to identification of several categories of new antigens recognized by the immune system of cancer patients, which are referred to as the cancer immunome. We analyzed normal testis cDNA expression libraries with serumobtained from non-small lung cancer patient and isolated 40 distinct antigen designated KP-LuT-1 through KP-LuT-40. Among these antigens 20 antigens were previously identified by SEREX analysis of other tumor types, and 20 out of 40 antigens (50%) did not match entries in Cancer Immunome Database and were considered newly identified antigens. Sequencing analysis showed that the anti-gens comprised 26 functional known proteins and 14 noble/uncharacterized gene products. Of these, the hypothetical protein KP-LuT-6 was shown tissue-restricted. RT-PCR showed it to be expressed strongly only in normal testis. In addition to normal tissues-restricted expression, KP-LuT-6 mRNA was detected in lung tumor samples(3/l0), stomach tumor samples(3/l0), and breast tumor samples(l/5), whereas not detected in colon tumor samples(O/I2). These data suggest that KP-LuT-6 is a cancer/testis (CT)-like antigen as a potential target for cancer immunotherapies.
GATA-3 is a Key Factor for Th1/Th2 Balance Regulation by Myristicin in a Murine Model of Asthma
Lee, Kyu ; Lee, Chang-Min ; Jung, In-Duk ; Jeong, Young-Il ; Chun, Sung-Hak ; Park, Hee-Ju ; Choi, Il-Whan ; Ahn, Soon-Cheol ; Shin, Yong-Kyoo ; Lee, Sang-Yull ; Yeom, Seok-Ran ; Kim, Jong-Suk ; Park, Yeong-Min ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1090~1099
DOI : 10.5352/JLS.2007.17.8.1090
Myristicin, l-allyl-3,4-methylenedioxy-5-methoxybenzene, was one of the major essential oils of nutmeg. However, its anti-allergic effect in the Th1/Th2 immune response was poorly understood. Recently, it was shown that T-bet and GATA-3 was master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether myristicin regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in ovalbumin (OVA)-induced asthma model mice. Myristicin reduced levels of IL-4, Th2 cytokine production in OVA-sensitized and challenged mice. In the other side, it increased
, Th1 cytokine production in myristicin administrated mice. We also examined to ascertain whether myristicin could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, and the development of airway hyper-responsiveness (AHR). The administration of myristicin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, these findings provide new insight into the immunopharmacological role of myristicin in terms of its effects in a murine model of asthma.
Research of Antioxidant Activity from Plant Resources using Proton Beam(I)
Moon, Byung-Sik ; Son, Gui-Youp ; Choi, Jin-Kuk ; Seo, Dong-Won ; Lee, Kap-Duk ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1100~1103
DOI : 10.5352/JLS.2007.17.8.1100
In proton therapy, the Bragg peak is spread out by modulating or degrading the energy of the particles to cover a well-defined target volume at a given depth. Proton transfer plays a key role in a variety of biological, the origin of the elements, tests of the standard model along with applications in medicine, industry and chemical phenomena such as water autoionization, fast proton diffusion, acid-base neutralization. We have studied the radiolysis of various natural resources and have evaluated the antioxidant activity of radiolysis products by proton beam. The most of antioxidant activities of natural resources were decreased with increasing proton fluence. Proton beam induced antioxidant activities both in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and 2,2`-azinobis(3-ethylbenzot hialozinesulfonic acid) cation radical
assay by a dose dependent fashion.
The Influence of Muddy Water in Imha Reservoir on the Ichthyofauna and Fish Growth
Han, Seung-Cheol ; Lee, Hak-Young ; Seo, Eul-Won ; Shim, Jae-Hun ; Lee, Jong-Eun ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1104~1110
DOI : 10.5352/JLS.2007.17.8.1104
The number of investigated fish collected from the Andong and the Imha reservoir were 2,540 individuals 20 species belonging to 7 families, 850 individuals 11 species belonging to 4 families respectively from May 2005 to March 2006. The total number of the common 3 species individuals investigated caught from the Andong reservoir and the Imha reservoir were 486 individuals and 124 individuals respectively. The investigated individuals is similar(
mm) to 171 individuals make a comparative study. The Opsariichthys uncirostris, Culter brevicauda, Hemiculter eigenmanni of length-weight relationship were TW
Volatile Flavor Components of Buckwheat-Green Tea
Choi, Sung-Hee ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1111~1114
DOI : 10.5352/JLS.2007.17.8.1111
The volatile flavor components of buckwheat (Fagopyrum esculentum Moench)-green tea were analyzed and identified. To make tea having good flavor and functional property, parched buckwheat (50%) was mixed with green tea (50%). The extraction of volatile flavor compounds of buckwheat-green tea was accomplished by a simultaneous distillation and extraction method using a Likens and Nickerson`s extraction apparatus. The concentrated extract was analyzed and identified by gas chromatography and GC-mass spectrometry. The main volatile flavor components of buckwheat-green tea were compounds that originated from parched buckwheat and the green tea. The former were 15 pyrazines having roasted and nutty aroma and methylbutanals and furfural having sweet-aroma. The latter were nerolidol, linalool, indole,
and geraniol etc having flower-like odor in green tea.
Analysis of Differentially Expressed Genes by Resveratrol Using Membrane Microarray
Kim, Jong-Sik ; Jang, Min-Jung ; Kim, Hyo-Eun ; Kim, Soon-Young ; Kim, Byung-Oh ; Sohn, Ho-Yong ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1115~1120
DOI : 10.5352/JLS.2007.17.8.1115
In the present study, we investigated whether several phytochemicals (resveratrol, genistein, epicatechin gallate, dially disulfide, caffeic acid phenetyl ester) and sulindac sulfide could induce expression of tumor suppressor p53 protein in human colorectal HCT116 cells. We found that p53 was dramatically induced by all phytochemical treatments except sulindac sulfide. Among treated phytochemicals, we selected resveratrol for further experiments because it is one of the highest p53 inducer. Using a Western blot analysis, we found that resveratrol induced p53 in a dose- and time-dependent manner. Additionally, using membrane-based microarray analysis, we found that twenty-five genes were up-regulated and two genes were down-regulated by resveratrol treatment. Among the up-regulated genes, we selected 4 genes and performed reverse-transcription-PCR to confirm microarray data. The results of RT-PCR were highly accorded with those of membrane microarray. In addition, we found that thrombospondin-1 (TSP-1) expression was not dependent on p53 presence, whereas mammary serine protease inhibitor (MASPIN) expression was dependent on p53 expressed by resveratrol treatment. The results of this study may help to promote our understandings of the molecular mechanisms of chemoprevention that are mediated by resveratrol in human colorectal cancer.
Antimicrobial and Anticancer Activity of Korean Traditional Soy Sauce and Paste with Chopi
Kim, Keun-Ki ; Park, Hyean-Cheal ; Son, Hong-Joo ; Kim, Yong-Gyun ; Lee, Sang-Mong ; Choi, In-Soo ; Choi, Young-Whan ; Shin, Teak-Soon ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1121~1128
DOI : 10.5352/JLS.2007.17.8.1121
The fruits of Zanthoxylum piperitum are known as having various physiology vitality, and the abstraction ingredient of the pericarp is also known as having strong antibiotic activities against various bacteria. Therefore, this study was carried out to estimate the effect of physiology vitality when the abstraction ingredient of Z. piperitum was added in soy sauce(Chopi-kanjang) and soybean paste(Chopi-doenjang). For the antibiotic activity against the pathogens of sitotoxism such as Staphylococcus aureus, Salmonella typhimurium, Vibrio parahemolyticus, Escherichia coli 0157:H7, the extracts of the Chopi-kanjang was added 1%, 2%, 4% pericarp of Z. piperitum in the manufacturing process of soy source. According to the results, the growth of E. coli 0157:H7 and V. parahemolyticus were respectively inhibited as 70% and 50% by the Chopi-kanjang added 2% of the ingredient. For the antibiotic effects of the aforementioned Chopi-kanjang against Sal. typhimurium and Sta. aureus, the growth of those pathogens was also inhibited between 40% and 60% according to the manufacturing period of Chopi-kanjang. It was confirmed that the antibiotic activity using the mixture of the abstraction ingredient and Chopi-doenjang was lower than those of Chopi-kanjang. In order to estimate the anticancer activity using by caspase-3 activity, the mixture of the abstraction ingredient of the pericarp of Z. piperitum and Chopi-kanjang was treated to leukemia cells. According to the results, the activities of caspase-3 using the mixture added 1%, 2% and 4% of the abstraction ingredient were respectively increased as much as 4, 12, 15 times comparing with the control which was treated with the soy source only. It could be that the mixture of the abstraction ingredient of the pericarp of Z. piperitum and soy source induced apoptosis, and the mixture of the abstraction ingredient and soybean paste had no effect on the activity of caspase-3. In order to find out the death of the aforementioned cells caused by necrosis or apoptosis, DNA fragmentation in the cell was examined. U-937 cells showed apoptotic DNA fragmentation in the incubation with Chopi-kanjang extract.
Bombyx mori Protein Disulfide Isomerase (bPDI) Protects Sf9 Cells from Endoplasmic Reticulum (ER) Stress
Goo, Tae-Won ; Yun, Eun-Young ; Kim, Sung-Wan ; Choi, Kwang-Ho ; Kang, Seok-Woo ; Kwon, Ki-Sang ; Kwon, O-Yu ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1129~1134
DOI : 10.5352/JLS.2007.17.8.1129
In the previous our study, a cDNA that encodes protein disulfide isomerase from Bombyx mori (bPDI)was isolated and characterized. bPDI has an open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and ER (endoplasmic reticulum) retention signal of the KDEL motif at its C-terminal. Recent studies have demonstrated that misfolded proteins are accumulated in many diseases including Alzheimer’s, goiter, emphysema, and prion infections. bPDI was over-expressed or knock-downed in Sf9 cells to study the relationship between bPDI expression and protections against protein misfolding. bPDI gene was cloned in insect expression vector pIZT/V5-His for over-expression and bPDI double-stranded RNA (dsRNA) was generated for knock-down. Over-expression of bPDI significantly improved survival rate, but bPDI dsRNA transfection significantly reduced survival rate after 48 hours exposure. In mock-transfected or wild-type cells had no significant effect. The results support the view that bPDI is one of the important intracellular components for cell protect mechanism, especially, against ER stress such as protein misfolding.
Antioxidative Activity and Nitrite Scavenging Effect of the Composites Containing Medicinal Plant Extracts
Cho, Hee-Sook ; Lee, Soo-Jung ; Shin, Jung-Hye ; Kang, Min-Jung ; Cho, Hyun-So ; Lee, Hyun-Ji ; Sung, Nak-Ju ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1135~1140
DOI : 10.5352/JLS.2007.17.8.1135
The composites(PM-A-PM-I) of 9 groups containing 7 kinds of hot water extracted medicinal plants were produced and evaluated its antioxidative activity. Each medicinal plants used these composites were analyzed in primer research that its anti oxidative activity was high. In the composites of medicinal plant, electron donating ability was higher than 70% in all sample. PM-D, PM-E and PM-F were significantly higher than others. Reducing power have similar tendency to electron donating ability. PM-D was the strongest in hydroxyl radical scavenging activity, followed by PM-E. In linoleic acid system, antioxidative activity of all sample was showed more than 50%, except PM-A. Especially PM-E and PM-F have significantly higher activity. Nitrite scavenging effect was significantly increased by PM-D, PM-E and PM-F added to Inula Japonica Thunberg. In these results, we suggested that high-er antioxidative activity of PM-D, PM-E and PM-F may be responsible for the contents of phenolic compounds present in Inula Japonica Thunberg. And thought to be enhanced by the synergy effect of the water extracted medicinal plants in the composite.
Studies on Antioxidant Activity and Inhibition of Nitric Oxide Synthesis of Germinated Brown Rice Soaked in Mycelial Culture Broth of Phellinus linteus
Jung, Il-Sun ; Kim, Yu-Jung ; Choi, In-Soon ; Choi, Eun-Young ; Shin, Su-Hwa ; Gal, Sang-Wan ; Choi, Young-Ju ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1141~1146
DOI : 10.5352/JLS.2007.17.8.1141
This study investigated the effects on the biological activities of germinated brown rice soaked in mycelial culture broth of Phellinus linteus. The level of free amino acid was higher in the GBRP extract than those of BR and GBR. The major free amino acids were alanine, valine, isoleucine and methionine in both extracts. The level of
-aminobutyric acid (GABA) was also increased significantly in the GBR and GBRP. Antioxidant activities of methanol extract of BR, GBR and GBRP were measured by using DPPH radical scavenging and SOD-like activity. Antioxidant activities showed the highest level of 83% and 76% when 100 mg/ml GBR and GBRP, respectively. Stimulation of the macrophages RAW264.7 cells with lipopolysaccharide (LPS) resulted in increased production of nitric oxide (NO) in the medium. However, the methanol extract of GBR and GBRP showed marked inhibition of NO synthesis in a does-dependant manner. These results showed that GBR and GBRP were significant role for activation of immune system in the pathogenesis of inflammatory diseases.
The Evaluation of Anti-wrinkle Effects in Oriental Herb Extract
Kang, Kum-Suk ; Kim, In-Deok ; Kwon, Ryun-Hee ; Heo, Ye-Young ; Oh, Sang-Hoon ; Kim, Min-Ah ; Jung, Hye-Jin ; Kang, Hwan-Yul ; Ha, Bae-Jin ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1147~1151
DOI : 10.5352/JLS.2007.17.8.1147
The human skin is constantly exposed to environmental irritants such as ultraviolet, smoke and chemicals. Free radicals and reactive oxygen species caused by them play critical roles in cellular damage. They not only injure the skin structure but also participate in the immensely complex inflammatory reaction. Anti-wrinkle effects of the Oriental herb extracts(OHE) were evaluated by the determination of anti-oxidation, collagenase inhibition and collagen synthesis in normal human fibroblast. OHE showed antioxidative activity as high as vitamin C, trolox and DL-penicillamine. Also OHE showed promotive effect on collagen synthesis and inhibitory effect on collagenase activity. These results demonstrated that OHE could be useful as an anti-wrinkle cosmetic ingredient.
Anti-Angiogenic and Anti-Cell Adhesion Effect of the Camellia japonica Flower Extract
Heo, In-Do ; Seo, Hyo-Jin ; Kim, Jong-Deog ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1152~1156
DOI : 10.5352/JLS.2007.17.8.1152
The Camella japonica flower(CJF) extract was studied for their anti-angiogenenic and anti-cell adhesion effect. CJF-extract inhibited the tube formation on human umbilical vein endotherial cells(HUVEC) with butanol extract by 70.2%, acetone extract by 54.2%, ethyl acetate extract by 37.0%, chloroform extract by 21.2%. Cell adhesion molecules were effectively suppressed at different concentration of CJF at 50, 100, 200 ug/well such as for intercellular adhesion molecule(ICAM) by 5.9%, 29.4% and 52.9%, for vascular cell adhesion molecule(VCAM) by 12.5%, 43.8% and 62.5%, for E-selectin by 7.1%, 21.4% and 35.7%, respectively. Signal molecules of vascular endotherial growth factor receptor 2(VEGFR2),
-catenin and PI3K are inhibited by different concentration of CJF at 10, 20 and 30
with western blot. Angiogenesis will be inhibited with suppressing NF-kB molecule resulted in signal molecules blocked by CJF. CJF will be useful materials for treatment of angiogenesis related diseases such as cancer, metastasis, rheumathioid arthritis and obesity.
Cloning and Expression Analysis of a Grape asr gene, VlASR Containing a Promoter Region.
Kihl, Joon-Yeong ; Pyee, Jae-Ho ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1157~1165
DOI : 10.5352/JLS.2007.17.8.1157
VvMSA, a grapevine ASR which is highly inducible by sugar and abscisic acid signals was previously shown to be a transcription factor for a hexose transporter gene VvHT1. We isolated a cDNA clone, VlASR which is regulated temporally during the grape berry development by ACP RT-PCR (annealing control primer reverse transcriptase-polymerase chain reaction) and it proved identical to VvMSA. RT-PCR and real-time PCR analyses revealed that the VlASR gene was expressed in berries at fruit set and that its expression increased as berries aged but decreased at the late ripening stage. In order to understand the regulatory mechanism of the asr gene, a genomic fragment was cloned from grapevine. The genomic DNA was 1375 bp long and a sugar box (sucrose box 3 and sucrose responsive element 1) was identified in the 611 bp upstream region of the open reading frame. Analysis of the VlASR promoter::reporter gene fusion demonstrated that this promoter was expressed in transgenic Arabidopsis even without sucrose treatment. This result suggests that the ASR/VvHT1-mediated sugar/ABA signaling, previously reported in grapevine, may not function in Arabidopsis which has no ASR homologue.
Immunological Aspects of Contemporary Exercise
Kwak, Yi-Sub ; Kim, Chul-Woo ; Paik, Young-Ho ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1166~1171
DOI : 10.5352/JLS.2007.17.8.1166
Exercise is the strongest stress to which the body is ever exposed. The body responds to this stress through a set of physiological changes in its metabolic, hormonal, and immunological systems. In this study, responses of the immune system to the long-term aerobic and anaerobic exercises have been investigated. Regular moderate exercise is associated with a reduced incidence of infection compared with a sedentary groups. Aerobic training increases the heart rate and enhances the body`s intake of oxygen long enough to benefit the condition of the body. In recent years, the importance of exercise in everyday life has been rapidly increasing. Moderate exercise appears to stimulate the immune system. And also, Exercise elicits an increase in the numbers of circulating lymphocytes and lymphocyte subsets (including NK cells) which is followed by a decrease in the numbers of cells during recovery from exercise. However, prolonged bouts of strenuous exercise cause a temporary depression of various aspects of immune functions (e.g. lymphocyte proliferation, monocyte antigen presentation, open window periods, exercise induced asthma, exercise induced anaphylaxis) that usually lasts 2-24 hr after exercise depending on the intensity and duration of the exercise bout. Exercise-induced bronchoconstriction (EIB) was defined as a decrease of at least 15% in pre exercise forced expiratory volume in one second at any time point after exercise. This includes elevation of cortisol and cathecholamines in plasma. On the other hand, highly trained athletes exhibit a chronic mild hypercortisolism at baseline that maybe an adaptive change to chronic exercise. And, Consuming carbohydrate during prolonged strenuous exercise attenuates rises in stress hormones and appears to limit the degree of exercise-induced immune depression. Recent evidence suggests that antioxidant vitamin supplementation may also reduce exercise stress and impairment of leukocyte functions.
Promotion of Aldehyde Oxidase Activities by Ethanol in Maize (Zea mays) Roots
Oh, Young-Joo ; Park, Woong-June ;
Journal of Life Science, volume 17, issue 8, 2007, Pages 1172~1175
DOI : 10.5352/JLS.2007.17.8.1172
We observed that exogenously applied ethanol changed the activities of aldehyde oxidases (AO) in the primary roots of maize (Zea mays). The stimulatory effect of ethanol on the aldehyde oxidase activities was concentration dependent; the AO activities were slightly weaker with 0.2 - 0.4% ethanol and stronger with 0.8 - 1.0% ethanol than the level of control. The promotion of AO activities was not explained by the increased transcription of two AO genes in maize. In contrast, ethanol strongly increased the amount of AO proteins, indicating that ethanol enhanced AO activities by promoting the translation. Among three alcohols including ethanol, methanol and isopropanol, only ethanol promoted AO activities. These results suggested that enhancement of AO activities was specific to ethanol, whose level could be naturally increased when the plant roots drove fermentation to overcome low oxygen stresses.