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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
Journal Basic Information
Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 17, Issue 12 - Dec 2007
Volume 17, Issue 11 - Nov 2007
Volume 17, Issue 10 - Oct 2007
Volume 17, Issue 9 - Sep 2007
Volume 17, Issue 8 - Aug 2007
Volume 17, Issue 7 - Jul 2007
Volume 17, Issue 6 - Jun 2007
Volume 17, Issue 5 - May 2007
Volume 17, Issue 4 - Apr 2007
Volume 17, Issue 3 - Mar 2007
Volume 17, Issue 2 - Feb 2007
Volume 17, Issue 1 - Jan 2007
Selecting the target year
Construction of a Transgenic Plant to Develop a New Method for the Isolation of Calmodulin-Binding Proteins
Kim, Sun-Ho ; Lee, Kyung-Hee ; Kim, Kyung-Eun ; Jung, Mi-Soon ; Lim, Chae-Oh ; Lee, Shin-Woo ; Chung, Woo-Sik ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1177~1181
DOI : 10.5352/JLS.2007.17.9.1177
Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety CaM-binding proteins (CaMBPs). Because eukaryotes have multiple CaMBPs, it is important to isolate and characterize them in different tissues and conditions. So far a number of CaMBPs have been identified through classical screening methods. Many classes of proteins have been predicted to bind CaMs based on their structural homology with already known targets. In an effort to develop a method for large-scale analysis of CaMBPs in Arabidopsis, we have generated a transgenic plants overexpressing AtCaM2-GFP. We performed protein pull-down assay to test whether exogenously expressed AtCaM2-GFP proteins can interact with CaMBPs. The exogenously expressed AtCaM2-GFP could strongly interact with a CaMBP, AS1 protein. This result suggests that AtCaM2-GFP in transgenic plants may interact with many CaMBPs in plant cell. Therefore, we will be able to isolate kinds of CaMBPs by using these transgenic plants in many different tissue and environments.
Cloning of Isoamylase Gene of Pectobacterium carotovorum subsp. carotovorum LY34 and Identification of Essential Residues of Enzyme
Cho, Kye-Man ; Kim, Eun-Ju ; Math, Renukaradhya K. ; Asraful Islam, Shah Md. ; Hong, Sun-Joo ; Kim, Jong-Ok ; Shin, Ki-Jae ; Lee, Young-Han ; Kim, Hoon ; Yun, Han-Dae ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1182~1190
DOI : 10.5352/JLS.2007.17.9.1182
The gene encoding for isoamylase of the Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 was cloned and expressed into Escherichia coli
. Isoamylase catalyzes the hydrolysis of
linkages specifically in amylopectin, glycogen, and derived oligosaccharides, while the enzyme did not hydrolyze
linkages of amylose. The isoamylase gene (glgX) had an open reading frame of 1,977 bp encoding 658 amino acid residues with a calculated molecular weight of 74,188 Da. The molecular weight of the enzyme was also estimated to be 74 kDa by activity staining of a SDS-PA gel. The mature GlgX had a calculated pI of 4.91. Isoamylase from Pcc LY34 had 70% amino acid identity with isoamylase from Pectobacterium chrysanthemi and contained the four regions conserved among all amylolytic enzymes. The isoamylase was optimally active at pH 7.0 and
. GlgX was
. The changes of Asp-335, Glu-370, and Asp-442 into Ala, respectively, using site-directed mutagenesis techniques showed that three residues are essential to isolamyalse (GlgX) activity. The sequences around those residues were highly conserved in isoamylase of different origins and GlgX of the glg operon in glycongen biosynthesis.
Cooperative Induction of HL-60 Cell Differentiation by Combined Treatment with Eugenol and 1α,25-Dihydroxyvitamin D
Oh, Mi-Kyung ; Park, Seon-Joo ; Kim, Nam-Hoon ; Cho, Jin-Kyung ; Jin, Jong-Youl ; Kim, In-Sook ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1191~1196
DOI : 10.5352/JLS.2007.17.9.1191
Eugenol (4-allyl-2-methoxyphenol) is a main component of essential oils obtained from various spices. Recent reports have shown that eugenol induces growth inhibition and apoptosis of malignant tumor cells. In this study, the stimulatory effect of eugenol on cell differentiation was investigated in HL-60 promyelocytic leukemia cells. When HL-60 cells were treated in combination with 150
of eugenol and 3 nM of
, cell growth was slower than that of cells treated with eugenol or
alone. Eugenol enhanced low dose of
phase arrest in cell cycle. Consistent with this, combined treatment of eugenol and
cooperatively increased p27 level and decreased cyclin A, cdk 2 and cdk 4 levels, which are cell cycle regulators related to
arrest. According to flow cytometric analysis, the expression of CD14 (monocytic differentiation marker) was more increased in the cells co-treated with eugenol and
. These results indicate that eugenol potentiates cell differentiation mediated by
of suboptimal concentration. The differentiation-inducing property of eugenol maybe contributes to chemopreventive activity of cancer.
Cloning, Sequencing and Expression of the Gene Encoding a Thermostable β-Xylosidase from Paenibacillus sp. DG-22
Lee, Tae-Hyeong ; Lee, Yong-Eok ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1197~1203
DOI : 10.5352/JLS.2007.17.9.1197
A genomic DNA library of the bacterium Paenibacillus sp. DG-22 was constructed and the
clones were identified using the fluorogenic substrate
. A recombinant plasmid was isolated from the clone and 4.3-kb inserted DNA was sequenced. The
gene (xylA) was comprised of a 2,106 bp open reading frame (ORF) en-coding 701 amino acids with a molecular weight of 78,710 dalton and a pI of 5.0. The deduced amino acid sequence of the xylA gene product had significant similarity with
classified into family 52 of glycosyl hydrolases. The xylA gene was subcloned into the pQE60 expression vector to fuse with six histidine-tag. The recombinant
was purified to homogeneity by heat-treatment and immobilized metal affinity chromatography. The pH and temperature optima of the
enzyme were pH 5.5-6.0 and
Anti-apoptotic Activity of Heme Oxygenase-1 Up-regulated by Etoposide in Human Retinal Pigment Epithelial Cells
Lee, Sang-Kwon ; Song, Ju-Dong ; Kim, Kang-Mi ; Kim, Jong-Min ; Lee, Sang-Yull ; Yoo, Young-Hyun ; Park, Young-Chul ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1204~1210
DOI : 10.5352/JLS.2007.17.9.1204
The topoisomerase II inhibitor etoposide causes an accumulation of DNA double strand breaks within the nuclei of cells. In this study, we investigated the effect of etoposide on the cell growth and apoptosis of human RPE cells. Etoposide evoked a significant inhibition of cell growth, and also induced DNA fragmentation in ARPE-19 cells. In addition, etoposide significantly up-regulated the expression of heme oxygenase-1 (HO-1), which is a stress-responsive protein and is known to play a protective role against the oxidative injury. And, etoposide-induced HO-1 expression was affected by the ROS scavenger N-acetyl cysteine. We also used oligonucleotides interfering with HO-1 mRNA (siRNA) for the inhibition of HO-1 expression. Interestingly, knock-down of the HO-1 gene significantly increased the level of DNA fragmentation in etoposide-treated ARPE-19 cells. In conclusion, these results suggest that up-regulated HO-1 plays as an anti-apoptotic factor in the process of apoptosis of ARPE-19 cells stimulated by etoposide.
Excretroy Urography in the Minipig
Jung, Joo-Hyun ; Chang, Jin-Hwa ; Choi, Min-Cheol ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1211~1216
DOI : 10.5352/JLS.2007.17.9.1211
Excretory urography was performed on eight healthy minipigs to evaluate radiographic anatomical status and excretive function of the urinary tracts and to get an optimal procedure. The right and left kidneys were located in
, respectively. The left kidneys were located more cra-nially than the right. The everage lengths and widths of kidneys were
cm on the ventrodorsal projection and
cm on the lateral projection, respectively. The lengths of kidneys were twice the width. When the lengths of kidneys were com-pared with lengths of the second lumbar vertebras, the ratios of kidney lengths to L2 lengths were
on the ventrodorsal projection and
on the lateral projection. The lengths and 쟝손 of renal pelvis including major calices were
cm, respectively. The lengths and 쟝손 of minor calices were
cm. The lengths and widths of the ureters were
cm. The nephrogram stage was reached in
minutes, and the pyelogram phase in
minutes individually after injection of contrast medium. The result of this study shows similar information and procedure of excretory urography for other laboratory ani-mals and leads to the potential use of this method for minipigs.
Comparison of Reproduction Systems of Genus Potentilla, Potentilla discolor in Korea and P. conferta in Mongol
Huh, Man-Kyu ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1217~1223
DOI : 10.5352/JLS.2007.17.9.1217
I investigated the reproduction system of nine natural populations of P. discolor in Korea and two Mongolian P. conferta populations. The measurements of 19 quantitative or qualitative morphological characters were taken on each of total individuals directly from their natural habitats. Multivariate principal component analyses (PCA) were conducted to detect differences among populations consid-ering several characters simultaneously of variances using the statistical analysis system. 19 morpho-logical characteristics between Korean Potentilla species and Mongolian Potentilla species showed a slight heterogeneity of variance. The length of internodes (LFL and LSI) and characteristics of root (LLR and NOR) were shown a significant difference between two species (P<0.05). The number of ra-mets in P. conferta decreased with increasing geographic distance from viviparity. However, P. discolor has most ramets at distance intervals
cm. In light conditions, P. discolor was significantly less resilience than P. conferta. In drought conditions, although there was not shown significant difference, P. conferta was less resilience than P. discolor. The core analysis indicates that P. conferta is the more resistant species than P. discolor and usually propagates by clonal growth during several strong envi-ronmental disadvantages such as drought events.
Effects of Glucose on Insulin-like Growth Factor Binding-5 Expression in Human Fibroblasts.
Ryu, Hye-Young ; Hwang, Hye-Jung ; Kim, In-Hye ; Ryu, Hong-Soo ; Nam, Taek-Jeong ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1224~1231
DOI : 10.5352/JLS.2007.17.9.1224
Insulin-like growth factor-I (IGF-I) and IGF-II have structure like insulin. In contrast to insulin, however, the bioavaility of IGFs is modulated by the IGF-binding protein (IGFBPs). Each of IGFBPs was different with molecular masses, biological characteristics, and immunological properties.. Human fibroblasts secrete IGFBPs that can modify IGF-I action. In diabetes mellitus, the most study of IGF systems have been investigated in insulin-dependent diabetes mellitus, non-insulin-dependent diabetes mellitus, and streptozotocin-in-duced animals in vivo. Recently, a little research regarding the IGFs system has been proposed in por-tion of cell in vitro. In this study, effects of low or high glucose condition on IGFBP-5 in GM10 was investigated. By western blotting analysis, IGFBP-5 level decreased in cells cultured at high glucose, but IGFBP-5 level of mRNA didn`t change. IGFBP-5 protease that cleaves IGFBP-5 in conditioned me-dium had was inhibited by EDTA and heparin, like serine protease and metalloprotease. Furthermore, the protease activity was increased in high glucose cultivated condition. In results of gelatin zymog-raphy, molecular weight of proteolytic metalloenzymes was indentified 69-kDa and protease activity was increased in time-dependent manner. Although the mechanism has yet to be determined, IGFBP-5 proteolysis in GM10 cells cultured with high glucose may increase effects of IGFs to decrease the glu-cose level through dissociation of IGFs from IGFBPs. Therefore, we suggest that IGF- I and IGFBPs could be potential models in study of pathophysiology such as diabetes mellitus.
Effect of Extracts from Root Bark of Ulmus parvifolia on Inhibition of Growth and DNA Synthesis of Human Cancer Cells.
Lim, Sun-Young ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1232~1236
DOI : 10.5352/JLS.2007.17.9.1232
Growth and DNA synthesis inhibitory effects of extracts from root bark of Ulmus parvifolia on MG-63 human osteosarcoma cells, HT-29 human colon cancer cells and K-562 leukemia cancer cells were studied. The root bark extract of Ulmus parvifolia was extracted with methanol, hot water and juice. The methanol extract showed the highest inhibitory effect on growth of MG-63, HT-29 and K-562 cancer cells by >85%. The treatment of hot water and juice extracts from root bark of Ulmus parvifolia also inhibited growth of the above cancer cells with increasing concentration. DNA synthesis of MG-63 and HT-29 cancer cells was significantly inhibited by adding methanol, hot water and juice extracts from root bark of Ulmus parvifolia with increasing concentration, showing that the inhibitory effect of growth was more effective on HT-29 cancer cells. These results suggest that the methanol extract from root bark of Ulmus parvifolia may have specific active com-pounds on anticancer effect. The hot water extract also showed a strong inhibitory effect on growth of cancer cells, indicating that the active compounds may be stable to heat.
Antioxidaive Activity of Lotus Root(Nelumbo nucifera G.) Extracts.
Lee, Jae-Joon ; Ha, Jin-Ok ; Lee, Myung-Yul ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1237~1243
DOI : 10.5352/JLS.2007.17.9.1237
This study was investigated on the antioxidant capacity of 80% ethanol extract of lotus root in vitro. The ex-traction yields of 80 % ethanol extract was 9.14%. Lotus root ethanol extract was fractionated by the following: n-hexane, chloroform, ethylacetate and n-butanol. n-Butanol fraction showed the highest ex-traction yield of all fractions. Antioxidative activities of different fractions were examined by l.l-diphenyl-2-picrylhydrazyl(DPPH) radical generation, the Rancimat test, the nitrite scavenging ac-tivity and the thiobarbituric acid(TBA) method, and compared with the properties of the commercial antioxidant butylated hydroxytoluene(BHT). The antioxidative capacity of the ethylacetate fraction was the highest among fractions and its fraction showed higher contents of total polyphenol. Furthermore, the antioxidative capacity of the ethylacetate fraction was similar to that of BHT. In conclusion, these results suggest that lotus root may be a good candidate as a natural antioxidant source.
The Change in Hematological Factors of Ascidian (Styela clava) Extract Diets Fed Rainbow Trout (Oncorhynchus mykiss).
Jeong, U-Cheol ; Lee, Jeong-Tae ; Palmos, Grace N ; Kang, Seok-Joong ; Choi, Byeong-Dae ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1244~1247
DOI : 10.5352/JLS.2007.17.9.1244
Effects of muscle fluid concentrates from Styela clava on fish diets were investigated in rainbow trout(Oncorhynchus mykiss). Fish were fed on one of the isonitrogenous(48%) and isolipic(20%) feed containing 5 to 20% of muscle fluid concentrates for 8 weeks. Hematological parameters such as hemoglobin, hematocrit, albumin, glucose, total bililubin, triglyceride and glutamic pyruvate transaminase of the rainbow trout which were fed on the diets varied, but no specific trend became apparent. However, glutamic oxaloacetic transaminase value was significantly higher than that of normal fishes. Therefore, concentrated ascidian fluid which is normally discarded, can be combined with commercial diets for the normal growth of rainbow trout.
Secretory Overexpression of Clostridium Endoglucanase A in Saccharomyces cerevisiae Using GAL10 Promoter and Exoinulinase Signal Sequeice.
Lim, Myung-Ye ; Lee, Jin-Woo ; Lee, Jae-Hyung ; Kim, Yeon-Hee ; Seo, Jin-Ho ; Nam, Soo-Wan ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1248~1254
DOI : 10.5352/JLS.2007.17.9.1248
The secretory overexpression of Clostridium thermocellum endoglucanase A gene (celA) was examined in Saccharomyces cerevisiae using Kluyveromyces marxianus exoinulinase (INU1) signal sequence and GAL10 promoter. The two plasmids, pYEG-CT1 with its own signal sequence, and pYInu-CT1 with INU1 signal sequence were introduced to S. cerevisiae SEY2102 and S. cerevisiae 2805 host strains, respectively, and then each transformant was selected on the synthetic defined media lacking uracil. The expression level and secretion efficiency of endoglucanase A was increased by
and 11%, respectively, by INU1 signal sequence over celA signal sequence. By considering the high level of expression (361 unit/I), plasmid stability (89%), and secretion efficiency (70%), S. cerevisiae 2805 harboring plasmid pYInu-CT1 was selected as the opti-mal host vector system for the production of cellulose-degrading enzyme and recombinant yeast probiotic. The total expression and secretion efficiency of endoglucanase A was 418 unit/l and 73%, respectively, in the batch fermentation of S. cerevisiae 2805/pYlnu-CT1 on galactose medium. The mo-lecular weight of secreted endoglucanase A was found to be greater than 100 kDa, presumably due to the N-linked glycosylation.
The Effect of Sampling Intervals on VO
Plateau and Reinvestigation of Other Criteria During VO
Yoon, Byung-Kon ; Kwak, Yi-Sub ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1255~1259
DOI : 10.5352/JLS.2007.17.9.1255
The purpose of this study was to elucidate incidence of
plateau by comparing data derived from different time averaging intervals during incremental cycling exercise to
. Seventeen subjects (age:
L/min, respectively) completed
tests on cycle ergometer which breath by breath gas ex-change data were obtained. These data were time-averaged into 11-breath, 15, 30 and 60 s sampling intervals. The incidence of plateau were 100, 35, 24 and 6% for the 11 breath, 15 s, 30 s and 60 s averaging, respectively. No correlation was between
Development of (α-Amylase Coated Magnetic Nanofiber for the Hydrolysis of Starch.
Kim, Hyun ; Lee, Jung-Heon ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1260~1265
DOI : 10.5352/JLS.2007.17.9.1260
Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of
nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.
Effects of Diaphragm Breathing and Garlic Powder Intake on Body Composition, Heart Rate, Blood Pressure and Immunoglobulin in Middle-aged Male smokers.
Choi, Seung-Uk ; Baek, Yeong-Ho ; Kwak, Yi-Sub ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1266~1271
DOI : 10.5352/JLS.2007.17.9.1266
The purpose of this study was to investigate combined effects of diaphragm breathing and garlic powder intake on body composition, heart rate, blood press and immunoglobulin levels in middle-aged male smokers from the age 40-49. Diaphragm breath training was 2-5 grade intensity on dyspnea scale for 20 minutes four times a week for 4 weeks and subjects were given garlic at 3 g of powder after breakfast and dinner two times a day during the 4 weeks. The conclusions of this study are as follows; Garlic intake group decreased in percentage of body fat, in the comparison between groups, garlic intake group had a lower percentage of body fat than control group. Heart rate was decreased in Diaphragm breathing group at rest. SBP was decreased in Diaphragm breathing+garlic intake group. Garlic intake group and diaphragm breathing+garlic intake group increased in IgG.
Effects of Different Substrate on the Growth and Microstructure of Fruit Body in the Basidiomycetes, Pleurotus ostreatus.
Ju, Young-Cheoul ; Yoon, Seon-Mee ; Kang, Hee-Wan ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1272~1277
DOI : 10.5352/JLS.2007.17.9.1272
Comparison of fruit bodies of Pleurotus ostreatus cultivar chunchu No .2 grown on the sawdust, rice straw and cotton waste substrates revealed differences in the pattern of differentiation of hyphal compartments. Required period for primordium induction of fruit bodies grown on sawdust substrate was 13 days. Physical structure shown as hardness of stipes grown on the sawdust substrate, fruit bodies were harden than control. Pileocystidia were well developed on the surface of pileus in the fruit body cultivated on rice straw. Microstructures of fruit body grown on the sawdust and cotton wastes substrates shown fast-discharge of basidiospore and sytoms ageing. Hyphae of fruit bodies formed on sawdust substrate had less stainable cytoplasmic material and many more vacuoles than hyphae of fruit bodies formed on synthetic substrate with 50% of pine sawdust, 30% of cotton seed hull and 20 of beet pulp(control).
Growth Inhibitions of Strains Exhibiting Resistances against General Disinfectants and Antibiotics by MBT-01108 Material.
Kim, Hae-Nam ; Park, Jin-Young ; Kim, Sam-Woong ; Jun, Hong-Ki ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1278~1283
DOI : 10.5352/JLS.2007.17.9.1278
The 30% resistant frequencies of pathogenic bacteria were identified against generally utilizing disinfectants. Among the used disinfectants, foodsef, Taego, and Iodo 175 were dictated by lower sensitivities against pathogenic bacteria, as well as higher resistant frequencies when compared with other disinfectants. The resistant frequencies against antibiotics were also dictated by 30% through MIC (minimal inhibitory concentration) and paper disc methods. Especially, the used bacteria exhibited resistances against gentamycin, kanamycin, and streptomycin, which included in all aminoglycoside group. The MBT-01108 material, which extracted and purified from a powder obtained by processing of Opunita ficus-indica var. saboten Makino trunk, did not develop or grow resistant bacteria. Interestingly, the multi-drug resistant bacteria such as MRSA, resistant Pseudomonas aeruginosa, VRE, and E. coli 0157 did not resistant against MBT-01108 material. These results suggest that MBT-01108 material uses as an anti-microbial agent.
Characteristics of Bacterial Community for Biological Activated Carbon(BAC) by Culturable and Unculturable Methods.
Park, Hong-Ki ; Jung, Eun-Young ; Jung, Mi-Eun ; Jung, Jong-Moon ; Ji, Ki-Won ; Yu, Pyung-Jong ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1284~1289
DOI : 10.5352/JLS.2007.17.9.1284
The Biological Activated Carbon (BAC) process in the water treatments represents a kind of biofiltration process which capabilities of bacteria to remove organic matters are maximized. It enables to eliminate organic matters and effectively reduce microbial regrowth potentials. As attached bacteria employ natural organic matter as a substrate, they are significantly dependent on indigenous microorganisms. In this study, characteristics of bacterial community by culturable and unculturable Methods have been conducted in a pilot plant using SAC in water treatment process at the downstream of the Nakdong River. Based on the results, HPC and bacterial- production for coal-based activated carbon material were
, respectively, in the SAC process. The highest level of attached bacteria biomass and organic carbon removal efficiency was found in the coal-based activated carbon. The genera Pseudomonas, Flavobacterium, Alcaligenes, Acilzetobacter, and Spingomonas were identified for each activated carbon material. Pseudomonas vesicularis was the dominant species in the coconut- and coal-based materials, where as Pseudomonas cepacia was the dominant species in the wood-based material. The Scanning Electron Microscope (SEM) observation of the activated carbon surface also found the widespread distribution of rod form and coccus. The community of attached bacteria was investigated by performing Fluorescent in situ hybridization (FISH) analysis. a group was dominant in coal, wood and coccunt-based materials,
group ranged from 27.0
28.6%, respectively. These results suggest that a group bacterial community appears to be regulated removal efficiency of organic material in water treatment process.
Analysis of Mineral, Amino Acid and Vitamin Contents of Fruiting Body of Sparassis crispa.
Shin, Hyun-Jae ; Oh, Deuk-Sil ; Lee, Hee-Duck ; Kang, Hyeong-Bong ; Lee, Chul-Won ; Cha, Wol-Suk ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1290~1293
DOI : 10.5352/JLS.2007.17.9.1290
The nutritional composition of fruiting body of Sparassis crispa has been analyzed for medicinal and edible uses. Minerals in S. crispa were found to be as follows; potassium (1,299.44 mg), phosphorus (104.73 mg), sodium (98.21 mg), magnesium (54.86 mg), calcium (8.39 mg), iron (7.61 mg), zinc (6.37 mg), copper (1.31 mg) and manganese (0.63 mg) based on 100 g of mushroom dry weight. In 20 kinds of total amino acids found in S. crispa, sum of glutamic acid and glutamine content was the highest (1,960 mg/l00 g) and sum of aspartic acid and asparagine, tryptophan, leucine and alanine were followed. Concerning free amino acids, glutamic acid, tryptophan, glutamine and aspartic acid were dominant. Among 8 vitamins detected, the vitamin E content was the highest (408.5 mg) based on 100 g of mushroom dry weight, then vitamin C, niacin and pantothenic acid were followed.
Study of Distance Relationships among Domestic Radish (Raphanus sativus L.) by Analyzing its Anti-fungal Protein Gene.
Hwang, Cher-Won ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1294~1297
DOI : 10.5352/JLS.2007.17.9.1294
To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fun- gal, anti-yeast activity were done against Bot교tis cenerea, Saccharomyces cerevisiaeι Candida albicans with it`s raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish`s anti- fungal protein.
(β-lapachone Regulates Tight Junction Proteins, Claudin-3 and -4, in Human Hepatocarcinoma Cells.
Kim, Sung-Ok ; Kwon, Jae-Im ; Kim, Gi-Young ; Kim, Nam-Deuk ; Choi, Yung-Hyun ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1298~1302
DOI : 10.5352/JLS.2007.17.9.1298
A hallmark of cancers is `leaky` tight junctions (Tjs). TJs mediated paracellular permeability is elevated and TJs maintained cell polarity is frequently lost. Concomitantly, TJs-associated proteins including members of the claudin family of proteins are dysregulated. Recent findings indicate that these TJs changes can contribute to cancer progression. In this study, we examined the effects of
, a quinone compound obtained from the bark of the lapacho tree (Tabebuia avellanedae), on the Tjs-associated regulators in human hepatocarcinoma cell lines, HepG2 and Hep3B.
treatment downregulated the levels of insulin-like growth factor 1 receptor (IGF-lR) proteins in both HepG2 and Hep3B cells. But the levels of claudin-3 and -4 proteins were increased in
-treated HepG2 and Hep3B cells. And also the zonnula occludens-l (la-I) and p-catenin protein levels by
were increased in a time-dependent manner. However, claudin-3 and -4 mRNA levels were uninhibited by
in HepG2 and Hep3B. The present results suggest that the upregulation of claudin-3 and -4 protein levels by
occurs by a post-transcriptional mechanism and points to a novel mechanism by
Interaction of FERM Domain of Tumor Suppressor, Merlin to its C-terminal Domain.
Oh, Jeong-Il ; Kang, Beom-Sik ;
Journal of Life Science, volume 17, issue 9, 2007, Pages 1303~1307
DOI : 10.5352/JLS.2007.17.9.1303
A tumor suppressor, merlin is a member of ERM family proteins. It consists of N-terminal FERM domain,
region, and C-terminal domain. Alternative splicing of merlin`s mRNA generates two isotypes of merlin. Isotype I, which has exon17 at the C-terminus instead of exon16 in isotype II, is known to have tumor suppressor activity. Like other ERM proteins, the C-terminal domain of merlin isotype I interacts to its FERM domain. That of isotype II, however, was reported not to bind FERM domain despite the large common part of C-terminal domain, which possibly binds FERM domain. Here, we show the binding of FERM domain to both C-terminal domains of merlin`s two isotypes by isothermal titration calorimetry. These results support that merlin isotype II also can form a closed conformation or a multimer by intramolecular or intermolecular interactions using their FERM domain and C-terminal domain.