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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
Selecting the target year
Effects of Exercise Training and Selenium on MCT1 and MCT4 Protein Levels in Skeletal Muscles of Diabetic Goto-Kakizaki Rats
Kim, Seung-Seok ; Kang, Eun-Bum ; Eum, Hyun-Sub ; Kim, Bum-Su ; Lim, Yea-Hyun ; Park, Joon-Young ; Cho, In-Ho ; Oh, Yoo-Sung ; Kwak, Yi-Sub ; Cho, Joon-Yong ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 1~8
DOI : 10.5352/JLS.2008.18.1.001
The purpose of this study was to determine the possible additive effects of endurance exercise training (EXER) and selenium (SELE) on the improvements of glucose and lactate transport capacities in diabetic Goto-kakizaki rats. Animals either remained sedentary control (SED) or performed EXER or received SELE [
kg body wt (-1) day (-1)], or underwent both EXER and SELE (COMBI), which lasted for 6 wk. Compared with sedentary control, EXER alone or the SELE alone group, or the combined treatment group had significant reduction in glucose response measured at 90 min and 120 min during an intraperitoneal glucose tolerance test (IPGTT) and body weight after 6week treatment. EXER alone, or combined group individually had significantly higher glycogen contents in liver compared with SED or SELE groups. EXER alone increased glycogen content in soleus and plantaris compared with SED, and this parameter was increased to greatest extent in the combined treatment groups compared with SED or SELE groups. EXER alone, SELE alone or COMBI, caused significant decreases in the plasma lactates, serum glucose, insulin, total cholesterol and HOMA-IR along with a significant increase in high-density lipoprotein cholesterol compared with SED. In addition, EXER or COMBI individually had significantly lower serum triacylglycerol compared with SED or SELE. With respect to protein expression related to glucose and lactate transport capacities, EXER alone, SELE alone, or COMBI increased in MCT1 and MCT4 protein level in soleus and plantaris. Furthermore, EXER alone, SELE alone or COMBI caused significant increases in mt MCT1 protein level in soleus and plantaris. The findings of the current study suggest that endurance exercise training and selenium treatment may provide therapeutic values to type II diabetic patients with peripheral insulin resistance and hyperlactatecemia by improving glucose and lactate transport capacities, leading to improvements in plasma lactate, serum glucose, insulin and lipid profiles (TC, TG, HDL).
Antioxidative Effects of Sulfur Containing Compounds in Garlic on Oxidation of Human Low Density Lipoprotein Induced by Macrophages and Copper Ion
Yang, Seung-Taek ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 9~15
DOI : 10.5352/JLS.2008.18.1.009
Sulfur containing compounds in garlic have all be used as one of the traditional folk medicine as well as food source. The present study was performed to investigate the antioxidative compounds of 1-methyl-1-cysteine, dimethyl trisulfide and 2-vinyl-4H-1,3-dithiin. The antioxidative activity of sulfur containing compounds on human LDL was investigated by monitoring a thiobarbituric acid substances (TBARS). Sulfur containing compounds inhibited on oxidation of LDL mediated by
and macrophages in dose dependent manner with almost completely inhibition at
. Antioxidant activities of sulfur containing compounds on LDL oxidation were 2-vinyl-4H-1,3-dithiin, 1-methyl-1-cysteine, and dimethyl trisulfide in order. Inhibitory effects of sulfur containing compounds on oxidation of LDL mediated by
and macrophages were degraded at much greater rate than native LDL, the LDL oxidation process was arrested as shown by the lower conjugated dienes formation at the concentration of
. Sulfur containing compounds in garlic revealed at high antioxidative activity at low physiological concentration for human LDL oxidation in vitro specially, it was indicated that the antioxidative activity of 3-viny l-4H-1,2-dithiin was higher than that of the other sulfur containing compounds.
HER-2/neu Protein Expression in Canine Mammary Adenocarcinoma
Yang, Hai-Jie ; Do, Sun-Hee ; Yuan, Dong-Wei ; Hong, Il-Hwa ; Ki, Mi-Ran ; Park, Jin-Kyu ; Goo, Moon-Jung ; Lee, Hye-Rim ; Hong, Kyung-Sook ; Hwang, Ok-Kyung ; Han, Jung-Youn ; Park, Ho-Yong ; Yoo, Sung-Eun ; Jeong, Kyu-Shik ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 16~22
DOI : 10.5352/JLS.2008.18.1.016
In this study to evaluate the involvement of EGFR, HER-2/neu and ALCAM (CD166) oncogene products in canine mammary neoplastic lesions, sections of archived paraffin-embedded samples of 49 mammary tumors were analyzed immunohistochemically using antibodies against human EGFR and HER-2/neu and ALCAM. These 49 tumors were divided into 2 groups: 22 benign (19 adenoma, 3 benign mixed tumors) and 27 malignant tumors (2 simple adenocarcinomas, 5 complex adenocarcinomas, 3 solid carcinoma, 5 sclerosing carcinoma, 8 malignant mixed tumors and 4 malignant myoepithelioma). As a result of immunostaining, 31.8% (7/22) of the benign tumors and 29.6% (8/27) of the malignant tumors expressed the HER-2/neu oncogene product, EGFR expression was detected in 27.3% (6/22) of benign tumors and in 22.2% (6/27) of the malignant tumors. ALCAM expression was detected in 40.9% (9/22) of benign tumors and in 7.4% (2/27) of the malignant tumors. These results suggest that some of the biological and morphological characteristics of the tumor are associated with canine mammary gland tumors, as also reported for human breast cancer, the possibility of using anti-HER-2/neu antibodies in the treatment of canine mammary tumors.
Ginsenosides Protect the High Glucose-induced Stimulation of IGFs in Mesangial Cells
Bae, Chun-Sik ; Lim, Do-Seon ; Yoon, Byeong-Cheol ; Jeong, Moon-Jin ; Yoon, Kyung-Chul ; Park, Soo-Hyun ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 23~29
DOI : 10.5352/JLS.2008.18.1.023
Panax ginseng C. A. MEYER is one of the most widely used herbal medicines in the Asian countries and has diverse functions including anti-diabetic action. The dysfunctions of mesangial cells in hyperglycemic conditions are implicated in the development of diabetic nephropathy. Insulin-like growth factors (IGFs) are also associated with the onset of diabetic nephropathy. Thus, we examined the effect of ginsenosides against high glucose-induced dysfunction of primary cultured rat mesangial cells. In the present study, high glucose increased IGF-I and IGF-II secretion in mesangial cells. Ginsenoside total saponin (GTS) prevented high glucose-induced increase of IGF-I and IGF-II secretion in mesangial cells. In addition, GTS prevented high glucose-induced increase of lipid peroxide formation and decrease of GSH contents. GTS also ameliorates high glucose-induced increase of arachidonic acid release and decrease of prostaglandin
. In conclusion, GTS prevented high glucose-induced dysfunction of mesangial cells via inhibition of oxidative stress and arachidonic acid pathways.
The Effect of Combined Exercise Training to Knee Joint Isokinetic Muscular Function in the Taekwondo Athlete
Choi, Kyung-Suk ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 30~37
DOI : 10.5352/JLS.2008.18.1.030
The purpose of this study was to investigate the effect of Isotonic Training and Isotonic isometric complex Training to Knee joint Isokinetic muscle function in the high-school Taekwondo athlete. This subjects were randomly divided into two groups; a Isotonic Training group (n
Physico-chemical Characteristics of Seasoned Pork Prepared with Medicinal Plant Extracts During Storage
Cho, Hee-Sook ; Shin, Jung-Hye ; Choi, Duk-Ju ; Lee, Soo-Jung ; Kang, Min-Jung ; Sung, Nak-Ju ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 38~45
DOI : 10.5352/JLS.2008.18.1.038
The seasoned pork loin manufactured using with seasoning sauce that were prepared with 3 different composites of medicinal plant extracts (
). The the quality characteristics were investigated during storage at
for 15 days. Sensory evaluation was higher in medicinal plant extracts added seasoning pork, in particular, significantly highest in group T-1. Lightness (
) value tended to decrease with increasing storage days, redness (
) was significantly higher in the medicinal plant composites added groups (T-1, 2 and 3) than control, yellowness (
) had no significant difference among three groups added medicinal paint composites. In hardness test, raw pork (control) was lower than seasoned pork groups, but tended to increase by added medicinal plant composites. Cooking loss increased with increasing storage period, but there was no significant difference between the seasoned pork groups (
). The changes of pH showed lower in control than seasoned pork groups (
) added seasoning sauce. Moisture content decreased with increasing storage period. VBN content increased during storage. After storage for 15 days, it was significantly higher in the order of T-3 >> T-0 > T-2 > T-1.
Effects of Punica granatum L. Extracts on Serum Lipids Level in Ovariectomized Rats
Park, Mi-Ra ; kim, Mi-Hyang ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 46~51
DOI : 10.5352/JLS.2008.18.1.046
This study investigated the effects of Punica granatum L. (PG) ethanol extracts on serum lipid levels in ovariectomized rats. In order to elucidate the effects of PG ethanol extracts on the change of lipid concentration in rats, Sprague-Dawley female rats were randomly assigned to groups as follows: sham-operated rats (sham), ovariectomized control rats (OVX-control), ovariectomized rats supplemented with ethanol extracts of PG seed at 50 mg/kg bw/day (OVX-PS50), ovariectomized rats supplemented with ethanol extracts of PG seed at 200 mg/kg bw/day (OVX-PS200), ovariectomized rats supplemented with ethanol extracts of PG peel at 50 mg/kg bw/day (OVX-PS50), ovariectomized rats supplemented with ethanol extracts of PG peel at 200 mg/kg bw/day (OVX-PP200). PG ethanol extracts were orally supplemented at the level of 1mL per day for 7 weeks. The body weight gain, food intake and food efficiency ratio were not significantly different between groups by t-test. The levels of total-cholesterol and triglyceride in serum were not changed by supplementation of PG ethanol extracts. The level of HDL-cholesterol on serum of the OVX-PS50 and OVX-PS200 groups were similar to the OVX-control group, but the OVX-PP50 and OVX-PP200 groups were higher than sham group. These results suggest that ethanol extracts of PG peel were more effected than those of PG seed.
Optimal Production of Xylooligosaccharide by Using Recombinant Endoxylanase from Bacillus subtilis
Kim, Yeon-Hee ; Heo, Sun-Yeon ; Kim, Mi-Jin ; Lee, Jae-Hyung ; Kim, Young-Man ; Nam, Soo-Wan ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 52~57
DOI : 10.5352/JLS.2008.18.1.052
Xylan is a major hemicellulose component of the cell walls of monocots and hardwood, representing up to 30% of the dry weight of these plants. To efficiently hydrolyze xylan, the endoxylanase gene from Bacillus sp. was expressed in B. subtilis DB431 by introducing the plasmid pJHKJ4. The total activity of the recombinant endoxylanase reached about 857 unit/ml by batch fermentation of B. subtilis DB431/pJHKJ4 in LB maltose medium. The majority (>92%) of endoxylanase was efficiently secreted into the culture medium. The recombinant endoxylanase hydrolyzed more the birchwood xylan efficiently than the other xylans. When 4 % concentration of xylan was used, the highest production of xylooligosaccharide was observed, and xylobiose and xylotriose were the major products. Optimal amount of enzyme and reaction time for producing xylooligosaccharide were found to be 10 unit and 1 hr, respectively. In addition, the temperature of
gave the highest production of xylooligosaccharide. Consequently, the optimized conditions for the production of xylooligosaccharide through the hydrolysis of xylan were determined as follows: 10 unit endoxylanase,
, 4% birchwood xylan, 1 hr reaction.
Isolation and Characterization of a Marine Derived Bacterium Glaciecola sp. SL-12 Producing β-agarase
Lee, Dong-Geun ; Lee, Ok-Hee ; Jang, Hyo-Jung ; Jang, Min-Kyung ; Yoo, Ki-Hwan ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 58~62
DOI : 10.5352/JLS.2008.18.1.058
A novel agar-degrading bacterium SL-12 was isolated from seashore of Kijang at Busan, Korea, and cultured in marine broth 2216 media. Isolated bacterium SL-12 was identified as Glaciecola genus by 16S rDNA sequencing with 98% identity. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was
. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is
-agarase. Thus, isolated bacterium and the enzyme would be useful for the industrial production of neoagarobiose.
Changes in the Quality of Beef Jerky Containing Additional Pine Needle or Mugwort Juice during Storage
Jung, In-Chul ; Park, Hyun-Suk ; Lee, Kyung-Soo ; Moon, Yoon-Hee ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 63~68
DOI : 10.5352/JLS.2008.18.1.063
This study was carried out to investigate the effect of the addition of pine needle juice and mugwort juice on the color, hardness, pH, VBN content, TBARS value and total plate count of beef jerky during storage at room temperature for 60 days. Beef jerky was prepared by three types such as beef jerky containing 50 ml water (control), beef jerky containing 50 ml pine needle juice (PBJ) and beef jerky containing 50 ml mugwort juice (MBJ). The L (lightness) value of the control and PBJ was not significantly changed during storage, but the
value of MBJ tended to increase during storage (p<0.05). The
(redness) value had the highest at the 60 day storage, and the
value of PBJ and MBJ were higher than that of the control (p<0.05). The
(yellowness) had the highest at the 60 day storage (p<0.05), and the
value was not significantly different among samples. The addition of pine needle juice and mugwort juice had no effect on the changes of color of beef jerky. The hardness tended to increase during storage period, the hardness of the control was higher than those of PBJ and MBJ until storage for 30 days (p<0.05). The pH of samples tended to decrease during storage period (p<0.05). The VBN content of samples tended to increase during storage period, and the VBN content of the control was higher than those PBJ and MBJ on the storage of 60 days (p<0.05). The TBARS value of samples tended to decrease during storage period, and the TBARS value of MBJ had the lowest during storage period (p<0.05). The total plate count tended to decrease during storage period (p<0.05), and the total plate count of control, PBJ and MBJ were 4.93, 4.11 and 4.17 log CFU/g, respectively.
Characteristics and Antimicrobial Resistance Patterns of Staphylococcus aureus Isolated from Horse
Choi, Seong-Kyoon ; Cho, Gil-Jae ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 69~74
DOI : 10.5352/JLS.2008.18.1.069
This study carried out to investigate the antimicrobial resistance and biochemical characteristics of Staphylococcus aureus (S. aureus) isolated from reproductive and respiratory tract in Thoroughbred horse. The specimens were collected from equine vaginal mucosa and upper respiratory tract from March to December 2006 using a culture swab in Korea. S. aureus suspected colonies on blood agar plates were selected and identified as standard biochemical tests and PCR (Applied Biosystems, USA). Antimicrobial resistance test of S. aureus isolates was performed with 30 antimicrobial agents (BBL, USA) by using the agar disk diffusion method. S. aureus isolates were isolated 58 (39.2%) strains of 148 samples: wound 64.7% (11/17), genital discharge 37.0% (37/100) and nasal discharge 32.2% (10/31). Almost isolates showed high resistance to spectinomycin, sulfonamides, erythromycin, tetracyelin, ciprofloxacin and penicillin. These results may provide the basic information to establish strategies for treatment and prevention of reproductive and respiratory disease in Thoroughbred horses in Korea.
Effects of Inhibitors on the Function and Activity of Topoisomerase, and Gene Expression in HL-60 Human Leukemia Cells
Jeong, In-Cheol ; Cho, Moo-Youn ; Park, Jang-Su ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 75~83
DOI : 10.5352/JLS.2008.18.1.075
This studies were designed to elucidate whether inhibitors of topoisomerase regulate function and activity of topoisomerase, and gene expression in HL-60 human leukemia cells. HL-60 cells were treated with 10-hydroxycamptothecin or doxorubicin, total RNA was isolated, and expressed genes were investigated with human oligonucleotide microarray containing 10K gene, respectively. Expression profiles of the human leukemia HL-60 cells treated with 10-hydroxycamptothecin (10-CIT) or doxorubicin associated with signal transduction,. cell adhesion, cell cycle, cell growth, cell proliferation, cell differentiation, transcription and immune response, especially genes related with transcription and cell growth. In HL-60 cells treated with 10-CPT, the expression of topoisomerase III
and I gene from oligo chip microarray analysis were increased over, but the expression of topoisomerase II
gene were decreased over. In contrast, the expression of topoisomerase II
gene were increased over in HL-60 cells treated with doxorubicin, whereas the expression of topoisomerase III
mRNA remained no significant change. These results suggest that these data may be useful for novel therapeutic markers.
Characterization of Pseudomonas sp. MN5 and Purification of Manganese Oxidizing Protein
Lee, Seung-Hui ; Park, Kyeong-Ryang ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 84~90
DOI : 10.5352/JLS.2008.18.1.084
Bacterial colonies which were able to oxidize the manganese were isolated from six soil samples in Byungchon area. Among them, one bacterial strain was selected for this study based on its high manganese oxidation activity. This selected bacterial strain was identified as Pseudomonas sp. MN5 through physiological-biochemical test and analysis of its 16s rRNA sequence. This selected bacterial strain was able to utilize fructose and maltose, but they doesn`t utilizing various carbohydrates as a sole carbon source. Pseudomonas sp. MN5 showed a very sensitive to antibiotics such as kanamycin, chloramphenicol, streptomycin and tetracycline, but a high resistance up to mg/ml unit to heavy metals such as lithium, manganese and barium. Optimal manganese oxidation condition of Pseudomonas sp. MN5 was pH 7.5 and manganese oxidation activity was inhibited by proteinase K and boiling treatment. The manganese oxidizing protein produced by Pseudomonas sp. MN5 was purified by ammonium sulfate precipitation, HiTrap Q FF anion exchange chromatography and G3000sw
gel filtration chromatography. By sodium dodecyl sulfate polyacrylamide gel electrophoresis, three manganese oxidizing protein with estimated molecular weights of 15 kDa, 46.7 kDa and 63.5 kDa were detected. Also, it was estimated that manganese oxidizing protein produced by Pseudomonas sp. MN5 were a kind of porin proteins through internal sequence and N-terminal sequence analysis.
The Development of Cranial Cervical Ganglion Block in Beagle Dogs
Park, Woo-Dae ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 91~95
DOI : 10.5352/JLS.2008.18.1.091
In human, sympathetic nerve blocks with local anesthetics are widely used to treat a variety of diseases in the innervating regions. However, its procedure in dogs is difficult to approach and process repeatedly because of anatomically location. Therefore, this study was designed to develop a new technique of sympathetic nerve block in beagle dogs. Fifteen healthy beagle dogs, which did not show any neurologic abnormalities and disease, were used for the study. Radiograghs were taken after injected radiopaque material mixed with 2% lidocaine at the cranial cervical ganglion and injected methylene blue using the same percutaneous technique to verify the reliability of this newly developed technique. The successful block rate of the cranial cervical ganglion block was present in 80% of all dogs and the stained cranial cervical ganglions were shown in all dogs. The results show that this new technique of the cranial cervical ganglion block is a reliable and simple method that can be used for clinical studies in dogs.
Induction of S Phase Arrest of the Cell Cycle by Piceatannol is Associated with Inhibition of Telomerase Activity in Human Leukemic U937 Cells
Choi, Yung-Hyun ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 96~102
DOI : 10.5352/JLS.2008.18.1.096
Piceatannol is a polyphenol that is found in abundant quantities in grapes and wine. Although recent experimental data revealed the anti-cancer potency of piceatannol, the molecular mechanisms underlying the antileukemic activity have not yet been studied in detail. In the present study, we investigated further possible mechanisms by which piceatannol exerts its anti-proliferative action in cultured human leukemia U937 cells. Exposure of U937 cells to piceatannol resulted in growth inhibition and induction of apoptosis as measured by MTT assay and flow cytometry analysis, which was associated with S phase arrest of the cell cycle. Piceatannol treatment markedly inhibited the activity of telomerase, and the levels of human telomerase reverse transcriptase (hTERT) and telomerase-associated protein-1 (TEP-1), main determinants of the telomerase enzymatic activity, were progressively down-regulated by piceatannol treatment in a dose-dependent fashion. However, the levels of cyclooxygenases (COXs) expression and prostaglandin E2 (PGE2) release were not changed in piceatannol-treated U937 cells. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of piceatannol.
Purification and Properties of a Novel Extracellular Agarase from Marine Bacterium, Sphingomonas paucimobilis AS-1
Jung, Il-Sun ; Kim, Yu-Jung ; Song, Hyo-Ju ; Gal, Sang-Wan ; Choi, Young-Ju ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 103~108
DOI : 10.5352/JLS.2008.18.1.103
An agar-degrading marine bacterium, strain AS-1 was isolated from the seawater. The strain AS-1 was identified as Sphingomonas paucimobilis (90% probability) by VITEK. The optimum medium for agarase activity of the isolated strain was determined to be marine medium, marine broth 2216 containing 0.1% agar as carbon source. An extracellular agarase was purified 104-fold from the culture supernatant by ammonium sulfate precipitation, ion exchange chromatography and gel filtration methods. The molecular weight of the purified enzyme was estimated to be 80 kDa by SDS-PAGE. The optimum pH and temperature for activity were 7.0 and
, respectively. Antioxidative activity of the strain AS- was 72% in the supernatant cultured for 12 h. The culture supernatant of the strain AS-1 showed antibacterial activity against bacteria causing putrefaction and food poisoning such as Escherichia coli, Staphylococcus aureus and Proteus vulgaris. However, the cell growth of the lactic aicd forming strain, Lactobacillus plantarium was promoted by the treatment of 10% culture supernatant of an agar-degrading strain.
Changes of Biological Activites of Two Ascidians, Styela clava and Styela plicata, by Cooking
Cha, Yong-Jun ; Lee, Hee-Young ; Jeong, Eun-Jeong ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 109~113
DOI : 10.5352/JLS.2008.18.1.109
For useful basic data in new ascidian processed products, this study investigated and compared the biological activities of two ascidians, Styela clava and Styela plicata, in two conditions with fresh one and cooking one at
for 10 min. By fresh state, Styela clava (8.46 mg/ml) had higher activity than Styela plicata (13.67 mg/ml) in the antioxidative assay (
), whereas Styela plicata (10.54 mg/ml) had higher than Styela clava (12.05 mg/ml) after cooking. Particularly the antioxidative activity of Styela plicata increased 1.3 times after cooking. Fresh Styela clava had antimicrobial activity against Bacillus subtilis, B. cereus, E. coli, whereas the activity appeared against E. coli only after cooking. However, antimicrobial activity in Styela plicata appeared against 4 gram positive bacteria (B. subtilis, B. cereus, S. aureus and L. monocytogenes) and E. coli, regardless of concentration level (1,000-1,500 ppm) and with/without cooking. ACE inhibitory activity was not observed in the samples. By cooking, the level of fibrinolytic activity increased to 1.79 from 1.24 plasmin unit/ml in Styela plicata, whereas the level decreased to 1.55 from 1.62 in 5tyela clava.
The Roles of Amino and Carboxyl Domains in the Mouse Wee1 Kinases
Han, Seung-Jin ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 114~119
DOI : 10.5352/JLS.2008.18.1.114
The molecular machinery controlling cell cycle is centered around the regulation of the activity of maturation-promoting factor (MPF), a complex composed of a catalytic Cdc2 and the cyclinB regulatory subunit. Cdc2 kinase is inactivated by phosphorylation of inhibitory kinase, Wee1. It has been known that there are three different Wee1 kinases in the mammalian cell, Wee1A, Wee1B and Myt1. To investigate the regulatory mechanism of Wee1 kinases, the phosphorylation and degradation of Wee1A and Wee1B were checked in the Xenopus oocyte cell cycle. When Wee1 kinases were injected into frog oocyte, Wee1B was more stable than Wee1A. Wee1A and Wee1B kinase were phosphorylated by many kinases such as PKA and Akt. The roles of amino or carboxyl terminal in mouse Wee1A or Wee1B kinase were investigated using chimeric constructs. The degree of protein phosphorylation, degradation and cell cycle progression were different between chimeric constructs. The amino domain of Wee1A was implicated in the protein phosphorylation and degradation while amino domain of Wee1B and carboxyl domain of Wee1A were involved in the activity regulation. These results suggested that the domains of Wee1 kinase have different and significant roles in regulating the Wee1 kinases in the cell cycle progression.
Euphorbiae Immifusae Sensitizes Apoptosis of TRAIL-resistant Human Gastric Adenocarcinoma AGS Cells
Lee, Jae-Jun ; Shin, Dong-Hyuk ; Park, Sang-Eun ; Kim, Won-Il ; Park, Dong-Il ; Choi, Yung-Hyun ; Hong, Sang-Hoon ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 120~128
DOI : 10.5352/JLS.2008.18.1.120
The death ligand tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/ Apo1L is a cytokine that activates apoptosis through cell surface death receptors. TRAIL has sparked growing interest in oncology due to its reported ability to selectively trigger cancer cell death. Euphorbiae humifusae Wind has been used in traditional Oriental medicine as a folk remedy used for the treatment of cancer. However, the mechanism responsible for the anticancer effects of E. humifusae not clearly understood. Here, we show that treatment with subtoxic doses of water extract of E. humifusae (WEEH) in combination with TRAIL induces apoptosis in TRAIL-resistant human gastric carcinoma AGS cells. Combined treatment with WEEH and TRAIL induced chromatin condensation and sub-G1 phase DNA content. These indicators of apoptosis were correlated with the induction of caspase activity that resulted in the cleavage of poly (ADP-ribose) polymerase. Combined treatment also triggered the loss of mitochondrial membrane potential. Furthermore, co-treatment with WEEH and TRAIL down-regulated the protein levels of the anti-apoptotic proteins such as Bcl-2, Bcl-xL, XIAP and cIAP-1. Although more study will be needed to examine the detailed mechanisms, this combined treatment may offer an attractive strategy for safely treating gastric adenocarcinomas and the results provide important new insights into the possible molecular mechanisms of the anticancer activity of E. humifusae.
Effects of Methanol Extract from Terminalia chebulae on Renal and Pulmonary Toxicities Induced by Paraquat in Rats
Park, Jong-Ok ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 129~135
DOI : 10.5352/JLS.2008.18.1.129
Paraquat (1,1-dimethyl 4,4` -dipyridium dichloride; PQ) is a kind of herbicide. Terminalia chebulae (TC) has been used as a medicine in China and in Korea for treating illnesses such as diarrhea, collapsed anus, spasmodic, diphtheria, asthma etc.. This study was to examin new physiological activities of methanol extract of TC (TCM) on the toxicity of PQ. It was observed biochemical effects on the toxicity of PQ in kidney and lung tissues after treatment orally administered 100, 200, 300 mg/kg of TCM daily for two weeks. In the experiment related to the toxicity of PQ, we got following results: renal and pulmonary lipid peroxide contents, activities of aminopyrine N-demethylase, aldehyde oxidase and xanthine oxidase were significantly increased in control group as compared with normal group, in the treatment of TCM the values were decreased as compared with control group. Activities of superoxide dismutase, catalase and glutathione peroxidase which are free radical scavenging enzymes were also increased in control group as compared with normal goup, but were decreased in TCM group as compared with control group. Collagen content and glucose-6-phosphatase activity in lung tissue were increased in control group as compared with normal, but was decreased in TCM group as compared with control group. From these results, we concluded that TCM can playa role as an effective agent to decrease toxicity of PQ.
Production of Enantiopure Styrene Oxide by Recombinant Pichia pastoris carrying Double Expression cassette of Epoxide Hydrolase Gene
Kim, Hee-Sook ;
Journal of Life Science, volume 18, issue 1, 2008, Pages 136~142
DOI : 10.5352/JLS.2008.18.1.136
A recombinant Pichia pastoris carrying double expression cassette of Rhodotorula glutinis epoxide hydrolase(RgEH) gene was developed and used for preparing enantiopure (S)-styrene oxide from racemic mixture of styrene oxide. BglII restriction site of original RgEH gene (pPICZ B/RgEH #2) of previous report was mutated using PCR technique for the construction of double expression cassette containing promoter (
), EH gene and transcription terminator (
) in pPICZ C vector. Double expression cassette with RgEH was inserted into the chromosomal DNA of P. pastoris.
) on (R)-styrene oxide of P. pastoris with double expression cassette was about 6-fold higher than that (
) of P. pastoris with single expression cassette. For the determination of the optimal condition, the effects of detergent and temperature on the enantioselective hydrolytic activity and yield of the enantiomer were investigated. When the reaction was performed at
for 10 min in the presence of 0.5% Tween 20, enantiopure (S)-styrene oxide with 99.9% ee was obtained as the yield 43.4 % from 20 mM racemic sustrate.