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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
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Spatial Autocorrelation Analysis among Subpopulations of Salix koriyanagi in Swampy Area at the Namgang River, Korea
Huh, Man-Kyu ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1325~1330
DOI : 10.5352/JLS.2008.18.10.1325
Salix koriyanagi is a deciduous shrub and native to Korea. The spatial distribution of multilocus allelic frequencies and geographical distances of the natural population in upper swampy area at the Namgang River in Korea were studied. The species showed a significant positive and negative spatial autocorrelation according to geographical distances as measured by Moran's I. Genetic similarity of individuals was found among subpopulations at up to a scale of a 12 m distance, and this was partly due to a combination of allelic frequencies, and therefore, a significant spatial autocorrelation was composed of a scale of 12 m intervals. Within S. koriyanagi in swampy area at the Namgang River, a strong spatial structure was observed for allozyme markers, indicating a migration within subpopulations.
Isolation and Characterization of Klebsiella pneumoniae WL-5 Capable of Decolorizing Triphenylmethane and Azo Dyes
Wu, Jing ; Lee, Young-Choon ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1331~1335
DOI : 10.5352/JLS.2008.18.10.1331
A Klebsiella pneumoniae WL-5 with the capability of decolorizing several recalcitrant dyes was isolated from activated sludge of an effluent treatment plant of a textile and dyeing industry. This strain showed a higher dye decolorization under static condition and color removal was optimal at pH 6-8 and
. More than 90% of its color of Congo Red were reduced within 12 hr at
dye concentration. Malachite Green, Brilliant Green and Reactive Black-5 lost over 85% of their colors at
dye concentration, but the percentage decolorization of Reactive Red-120, Reactive Orange-16, and Crystal Violet were about 46%, 25%, and 13%, respectively. Decolorizations of Congo Red and triphenylmethane dyes, such as Malachite Green, Brilliant Green, and Crystal Violet were mainly due to adsorption to cells, whereas azo dyes, such as Reactive Black-5, Reactive Red-120, and Reactive Orange-16 seemed to be removed by biodegradation through unknown enzymatic processes.
Amelioration of Asthmatic-Related Symptoms by an Aqueous Extract of Angelica archangelica L.
Heo, Jin-Chul ; Lee, Sang-Han ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1336~1341
DOI : 10.5352/JLS.2008.18.10.1336
Inflammation through the respiratory tract is a crucial event in immune disorders, including asthma, and atopic rhinitis. To investigate whether an aqueous extract of Angelica archangelica L. (AaL) has a beneficial influence in terms of anti-asthmatic activity, its effects on an ovalbumin-induced asthmatic model were examined. Mice sensitized to ovalbumin were orally administered the AaL extract, and their lungs examined by Haematoxylin-Eosin staining to determine IL-4/13 cytokine expression. The AaL extract exerted strong anti-asthmatic effects by regulating each level in the
cell number, IL-4/13, and other target markers in the lungs. Together, these results collectively indicate that the aqueous AaL extract ameliorates asthmatic symptoms effectively in a mouse ovalbumin-challenge model.
Effect of Silkworm (Bombyx mori) Excrement Powder on the Alcoholic Hepatotoxicit in Rats
Kim, Yong-Soon ; Kim, Kee-Young ; Kang, Pil-Don ; Cha, Jae-Young ; Heo, Jin-Sun ; Park, Bo-Kyung ; Cho, Young-Su ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1342~1347
DOI : 10.5352/JLS.2008.18.10.1342
The purpose of present study was to investigate the protective effect of silkworm excrement powder (SEP) on alcohol-induced hepatotoxicity in rats. Semisynthetic diet supplemented with SEP (3%, w/w) given to alcohol-feeding rats for 30 days, then blood and tissues were collected, processed and used for alcohol concentration mensuration, various biochemical estimations and histopathological examination. Chronic alcohol administration resulted in significantly increase in the activities of the clinically important liver marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST),
-glutamyl transpeptidase (
-GTP) and lactate dehydrogenase (LDH). Also, a highly significant increase in the blood alcohol level by alcohol treatment was observed. But alcohol-induced elevation of ALT and LDH levels markedly prevented and the level of blood alcohol decreased in SEP treated rats as compared to alcohol-administered control rats. SEP supplementation showed highly decreased the concentrations of total lipid, triglyceride and cholesterol in serum, as compared with alcohol treated control rats. Alcohol treatment induced the marked accumulation of large lipid droplets, hepatocytes necrosis and inflammation in the liver, but SEP administration attenuated to alcohol-induced accumulation of lipid droplets and hepatocyte necrosis. The results indicated that SEP may exert a protective effect against alcoholic hepatotoxicity through decreasing the activity of hepatic marker enzymes.
Alternative Isoforms of the mi Transcription Factor (MITF) Regulate the Expression of mMCP-6 in the Connective Tissue-Type Mast Cells Cultured with Stem Cell Factor
Lee, Sun-Hee ; Guan, Xiu-Ying ; Kim, Dae-Ki ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1348~1354
DOI : 10.5352/JLS.2008.18.10.1348
mi transcription factor (MITF) is important in regulating the differentiation of mast cells. In particular, MITF regulates the transcription of the mouse mast cell-specific serine protease (mMCP)-6 gene, which is generally expressed by the connective tissue-type of mast cells. In this study, we investigated alternative isoforms of MITF that regulate transcription of the mMCP-6 gene in bone marrow-derived cultured mast cells in mice. The expression of MITF isoforms was examined by RT-PCR. We observed that MITF-A, -E, -H and -Mc were expressed by mucosal-type mast cells cultured in the presence of IL-3, whereas the connective tissue-type mast cells cultured in the presence of stem cell factor (SCF) expressed MITF-A. Overexpression of MITF isoforms increased luciferase activity through the mMCP-6 promoter in NIH-3T3 cells and elevated the level of mMCP-6 expression in the MC/9 mast cell line. Moreover, mMCP-6 expression in mast cells was significantly inhibited by the depletion of MITF. The transcriptional activity and DNA binding of MITF-A was comparable to that of MITF isoforms, including MITF-E, -H, and -Mc. Our results therefore suggest that MITF-A may be an important isoform of MITF in regulating the transcription of mMCP-6 in mouse connective tissue mast cells.
Isolation of Endophytic Fungi Capable of Plant Growth Promotion from Monocots Inhabited in the Coastal Sand Dunes of Korea
Khan, Sumera Afzal ; Hamayun, Muhammad ; Rim, Soon-Ok ; Lee, In-Jung ; Seu, Jong-Chul ; Choo, Yeon-Sik ; Jin, Ing-Nyol ; Kim, Sang-Dal ; Lee, In-Koo ; Kim, Jong-Guk ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1355~1359
DOI : 10.5352/JLS.2008.18.10.1355
Endophytic fungi predominantly inhabit grasses, and produce a variety of beneficial metabolites for plant growth, as well as help their hosts against pathogens and herbivores. Current study was focused on plant growth promoting activity of endophytic fungi inhabited in the roots of sand dune grasses. We collected 49 fungal isolates from the roots of four most common sand dune grasses and screened them for their growth promoting capacity. Results showed that 37 fungal isolates (75.5%) promoted plant height and shoot length of waito-c rice, 11 fungal isolates (22.5%) suppressed it, while 1 fungus (2%) showed no effect on the growth attributes. The fungal strain Gibberella fujikuroi, along with distilled water and Czapek broth medium, were taken as control for this experiment. It was concluded that a major proportion of endophytic fungi inhabited in the sand dune plants produce metabolites, and thus help in growth and development of the host plant.
Antioxidative Effects of Pleurotus eryngii and Its By-products
Cho, Hyun-So ; Lee, Hyun-Ji ; Lee, Soo-Jung ; Shin, Jung-Hye ; Lee, Hyun-Uk ; Sung, Nak-Ju ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1360~1368
DOI : 10.5352/JLS.2008.18.10.1360
Physicochemical characteristics and antioxidative activity were measured to investigate the possibility for functional characteristics of Pleurotus eryngii and its by-products. By-products of Pleurotus eryngii were classified with mushroom, fungal body and fermented mushroom by-product. Moisture was the highest in fermented mushroom by-product and crude protein was 1.72%, in mushroom. Crude fiber content was less than 10% except the fungal body by-product. Mineral content appeared to be the highest in the fermented mushrooom with a value of 3,696.1 mg/100 g, and potassium was a predominant mineral in Pleurotus eryngii as well as its by-products. Amino acid content was the highest in mushroom with a level of 989.59 mg/100 g. DPPH radical scavenging ability of the fermented mushroom was the highest, and its methanol extract and water extract exhibited
of scavenging activity at a concentration of 10 mg/ml. Reducing power was significantly higher in the fermented mushroom in comparison with those of the mushroom, mushroom by-product, and fungal body by-product. The reducing power of the water extract of fermented mushroom was the highest with a value of
. SOD-like activities for the individual samples except the fungal body by-product were higher than 50% at a concentration of 10 mg/ml. The hydroxyl radical scavenging abilities of the individual samples except the fungal body by-product were over 50%. Nitrite scavenging effects were better in pH 2.5 than in pH 4.0. While the nitrite scavenging effects of methanol extracts were
, those of the water extracts were
. Antioxidative activity of the fermented mushroom appeared to be the highest among the mushroom by-products. Taken together, these results provide an insight into utilization of the mushroom by-products as materials for functional foods and animal feed.
Effects of Feeding Citrus by Products on Nutritional Components of Korean Native Chickens
Yang, Seung-Ju ; Jung, In-Chul ; Moon, Yoon-Hee ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1369~1376
DOI : 10.5352/JLS.2008.18.10.1369
In this study, the effects of feeding citrus byproducts on nutritional components of Korean native chickens were investigated. Two samples of Korean native chickens were used for this study: T0 (chickens fed with only feed for laying hen until they became 39 weeks old, not with citrus byproducts) and T1 (chickens fed with citrus byproducts). The feed for T1 was the same as T0 for the first 16 weeks, and then was made by adding 4% of citrus byproducts to the feed for T0 between
weeks. The chicken used for the experiment was obtained by chilling them for 2 days after slaughter. There was no significant difference between T0 and T1 regardless of feeding citrus byproducts, in terms of their breast/thigh's calorie, contents of moisture, protein, fat and ash, total structural amino acid, total free amino acid and composition of saturated/unsaturated fatty acids. The breast's cholesterol amount of T0 and T1 were 48.4 mg/100 g and 47.6 mg/100 g, respectively, while that of thigh for T0 and T1 were 75.7 mg/100 g and 72.8 mg/100 g, respectively, which implies that T1 showed lower amount of cholesterol than T0. T1 showed significantly higher amount of phosphorus, potassium, magnesium and vitamin B2 for thigh than T0 (p<0.05).
Process Optimization of Dextran Production by Leuconostoc sp. strain YSK. Isolated from Fermented Kimchi
Hwang, Seung-Kyun ; Hong, Jun-Taek ; Jung, Kyung-Hwan ; Chang, Byung-Chul ; Hwang, Kyung-Suk ; Shin, Jung-Hee ; Yim, Sung-Paal ; Yoo, Sun-Kyun ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1377~1383
DOI : 10.5352/JLS.2008.18.10.1377
A bacterium producing non- or partially digestible dextran was isolated from kimchi broth by enrichment culture technique. The bacterium was identified tentatively as Leuconostoc sp. strain SKY. We established the response surface methodology (Box-Behnken design) to optimize the principle parameters such as culture pH, temperature, and yeast extract concentration for maximizing production of dextran. The ranges of parameters were determined based on prior screening works done at our laboratory and accordingly chosen as 5.5, 6.5, and 7.5 for pH, 25, 30, and
for temperature, and 1, 5, and 9 g/l yeast extract. Initial concentration of sucrose was 100 g/l. The mineral medium consisted of 3.0 g
, 0.01 g
, 0.01 g
, 0.2 g
, 0.01 g NaCl, and 0.05 g
per 1 liter deionized water. The optimum values of pH and temperature, and yeast extract concentration were obtained at pH (around 7.0), temperature (27 to
), and yeast extract (6 to 7 g/l). The best dextran yield was 60% (dextran/g sucrose). The best dextran productivity was 0.8 g/h-l.
Effects of Aerobic Training Plus Diet on Blood Lipids and Apolipoproteins in Obese Children
Park, Tae-Gon ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1384~1389
DOI : 10.5352/JLS.2008.18.10.1384
The purpose of this study was to find out the effects of aerobic training plus diet on blood lipids and apolipoproteins (Apo) in obese children. Sixteen healthy obese boys (ages 10.9; body mass index (BMI)
95th percentiles for age and sex) participated in this study. The aerobic training program consisted of 40 minutes hiking on a mountain, 60 minute of basketball and football dribbling at an intensity of 60-70% of HRmax, and was performed 5 days a week for 9 weeks. The diet prescription was 2,100 kal/day according to the recommended dietary allowance for 10-12 year old Koreans. All subjects stayed in a training camp for 9 weeks. The results of this study were as follows; Blood lipid profiles including total cholesterol (TC), triglyceride (TG) high density lipoprotein cholesterol (HDL-C) and TC/HDL-C ratio were significantly improved after the 9 week program, but there was no significant change in low density lipoprotein (LDL-C). Apolipoprotein profiles, Apo AI, AII, B, CII and CⅢ were all significantly decreased after the 9 week program, but there were no significant difference in Apo AI/AII ratio and Apo B/AI ratio. These results indicate that aerobic training together with a healthy diet can induce positive changes on blood lipid profiles, Apo AII, B and CII in obese children.
Safety Test of Brown Rice Expressing Arabidopsis Calcium Transporter by Feeding Trial in Mice
Kim, Kyung-Min ; Kim, Chang-Kil ; Kim, Byung-Oh ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1390~1394
DOI : 10.5352/JLS.2008.18.10.1390
Previously, we found that the transgenic rice plants over-expressing the Arabidopsis
antiporter CAX 1 (accession no. U57411) gene accumulated 2.7 to 7.5-fold more calcium in the T3 rice grains as compared to those of control. To examine physiological safety of the
rice grains, the effect of the
brown rice on change in levels of body weight and white blood cells was compared with that of the control Ilpum brown rice by feeding trial in mice. During the feeding trial for one month, there was no significant difference between two mice groups, which were fed by the
brown rice or Ilpum brown rice. There were no detectable differences in their effects on immune functions including plaque-forming unit, peritoneal macrophage number, and NK-cell activity. In addition, biochemical analysis of the blood failed to exhibit any difference between two mice groups. Together, these results suggested that the
brown rice, which was produced from a genetically modified organism (GMO), might be safe and possess a potential to be applicable as calcium-fortified feed or food. Long-term safety of the
brown rice, however, remains to be elucidated.
A Cancer-specific Promoter for Gene Therapy of Lung Cancer, Protein Regulator of Cytokinesis 1 (PRC1)
Cho, Young-Hwa ; Yun, Hye-Jin ; Kwon, Hee-Chung ; Kim, Hee-Jong ; Cho, Sung-Ha ; Kang, Bong-Su ; Kim, Yeun-Ju ; Seol, Won-Gi ; Park, Kee-Rang ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1395~1399
DOI : 10.5352/JLS.2008.18.10.1395
We have recently reported the PRC1 promoter as a promoter candidate to control expression of transcriptionally targeted genes for breast cancer gene therapy. We tested whether the PRC1 promoter could be also applied for the lung cancer gene therapy. In the transient transfection assay with naked plasmids containing the luciferase fused to the PRC1 promoter, the promoter showed little activity in the normal lung cell line, MRC5. However, in the lung cancer A549 cells, PRC1 showed approximately 30-fold activation which was similar to the survivin promoter, the gene whose promoter has been already reportedas a candidate for the gene therapy of lung cancer. In viral systems, the PRC1 promoter showed approximately 75% and 66% of transcriptional activity compared to the CMV promoter in the adeno-associated virus (AAV) and the adenovirus (AV) systems, respectively. However, the PRC1 promoter in either AAV or AV showed approximately 20% activity compared to the CMV promoter in the normal lung cells. In addition, human lung tumor xenograft mice showed that the PRC1 promoter activity was as strong as the CMV activity in vivo. Taken together, these results suggested that PRC1 might be a potential promoter candidate for transcriptionally targeted lung cancer gene therapy.
A Study on the Arthropod Succession in Exposed Pig Carrion
Jung, Jae-Boung ; Yoon, Myung-Hee ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1400~1409
DOI : 10.5352/JLS.2008.18.10.1400
This study was carried out to offer the forensic entomological evidence to estimate the postmortem interval (PMI) based on the decomposing state of the pig carrion and the arthropod succession in exposed carrion. A total of 48 species of 28 families belonging to 7 orders were collected, and dominant insect varied with season, i. e., the Calliphoridae in March, May and October, both of the Calliphoridae and the Staphylinidae in July, and the Staphylinidae in September. The Calliphoridae was the first visiting insect throughout all the seasons as well as the index insect dominant in the early decomposing stage. The index insect in the later decomposing stage, however, was the Staphylinidae throughout all the seasons except in March. In March, the Muscidae was dominant, presumably because the flies consistently arrived throughout the extended period of decomposition of the carrion owing to the low ambient temperature of the season. In this connection, it is known that not only the decomposition stage of the carrion and the carrion-associated insect but also the climatological condition of the region have to be taken into consideration to estimate the PMI.
Analysis of Optimum Condition for Production of an Onionic Vinegar by Two-Step Fermentations
Kim, Sam-Woong ; Park, Jai-Hyo ; Jun, Hong-Ki ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1410~1414
DOI : 10.5352/JLS.2008.18.10.1410
This study was carried out to develop a vinegar by an onion juice. Onions are considered to be a promising source of the vinegar because these are rich in sugars, amino acids and various nutrients. An Acetobacter for an acetic acid fermentation was isolated and used from vinegars produced by industrial goods or from matured Kimchi. When supplemented with 2-8% ethanol into an onionic juice medium, the highest production of the acetic acid was observed at 9 days by addition of 4% ethanol. Optimum temperature and aeration for acetic acid production were exhibited at
and 200 rpm, respectively. A flask containing larger air-contact surface region for fermentation was produced the more acetic acid than that of a test tube. Taken all these together, an optimum condition for the acetic acid fermentation was over 9 days at
, 200 rpm with 5% alcohol and 2% initial acidity. When fermented by the upper condition, the final product contains 5.2% total acidity and less than 1% ethanol. These are suitable for conditions of fruit vinegar notified by the Ministry of Commerce, Industry and Energy.
Inhibitory Effects of Methanol Extracts from Korean Orysa sartiva and Coix lachryma-jobi var. ma-yuen on Mutagenicity and Growth of Human Cancer Cells
Lim, Sun-Young ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1415~1419
DOI : 10.5352/JLS.2008.18.10.1415
This study was carried out to determine the inhibitory effects of methanol extracts from Korean Orysa sartiva and Coix lachryma-jobi var. ma-yuen on mutagenicity using Ames test and growth of AGS human gastric adenocarcinoma and HT-29 human colon cancer cells. Both treatments of methanol extracts (5 mg/assay) from Orysa sartiva and Coix lachryma-jobi var. ma-yuen to Ames test system inhibited aflatoxin
) induced mutagenicity by 76%. In case of N-methyl-N'-nitro-N-nitrosoguamidine (MNNG) induced mutagenicity, the methanol extracts (5 mg/assay) from Orysa sartiva and Coix lachryma-jobi var. ma-yuen showed 79% and 69% inhibitory rate, respectively and the inhibitory effect was a little stronger in Orysa sartiva Inhibitory effects of methanol extracts from Orysa sartiva. and Coix lachryma-jobi var. ma-yuen on the growth of AGS and HT-29 human cancer cells were increased as dose dependent patterns and the inhibitory effects on AGS and HT-29 cells were similar. The above results indicate that the consumption of these cereals, which contain many nutrients with good quality, may be recommended as potent functional foods for improving health.
Fermentation Characteristics of Cheonggukjang made of Germinated Soybean under Light Condition
Kim, Mi-Hyang ; Lee, Nan-Hee ; Choi, Ung-Kyu ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1420~1425
DOI : 10.5352/JLS.2008.18.10.1420
This study was conducted to investigate the fermentation characteristics of cheonggukjang prepared with soybean germinated under light condition. The moisture content and pH of cheonggukjang made with soybean germinated under light condition (CGL) were higher than that of cheonggukjang made with non-germinated soybean (CNG). The color of CGL was darker than that of CNG and cheonggukjang made with soybean germinated under dark condition (CGD). The number of live bacteria in the CGL (
) and CGD (
) was higher than that of CNG (
). The levels of isoflavone in CGL, CGD and CNG were 2,038.0, 1,993.6 and 1,747.0 ug/g, respectively. There were no significant differences in the appearance, aroma, taste and overall acceptability of the cheonggukjang made with different germination conditions. Therefore, it was expected that the cheonggukjang prepared with soybean germinated under light condition could be practically used as a new fermented food.
Effects of Supplemental Synbiotics Composed of Anaerobic Bacteria, Yeast and Mold on the Aerobic Stability of Total Mixed Ration for Cattle
Lee, Shin-Ja ; Shin, Nyeon-Hak ; Jung, Ho-Sik ; Hyun, Jong-Hwan ; Moon, Yea-Hwang ; Lee, Sang-Suk ; Lee, Sung-Sill ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1426~1434
DOI : 10.5352/JLS.2008.18.10.1426
This study was conducted to investigate the effects of supplementation of synbiotics manufactured with anaerobic bacteria, yeast and mold on preservation of total mixed ration (TMR) by exposing days. Eight treatments were composed of untreated synbiotics(US), bacterial synbiotics (BS), yeasty synbiotics (YS), moldy synbiotics (MS), bacterial and mouldy synbiotics (BMS), yeasty and moldy synbiotics (YMS), bacterial and yeasty synbiotics (BYS), and bacterial, yeasty and moldy synbiotics (BYMS). After 7 days of anaerobic fermentation, fermented-TMRs were exposed to the air during 1, 3, 5, 7, 14 and 21 days. One hundred forty four (8 treatments
3 replications) fermented-TMRs were manufactured by vinyl bag (
). Although no significant differences in the activities of carboxymethylcellulase, xylanase and amylase were observed among treatments, theirs acivities were seemed to increase by treatment of BYS or YMS containing yeast. Total bacterial and mold counts also decreased in the treatments containing yeast. Potential pathogenic bacteria were less detected in BYS and BMYS for E. coli, BMYS and YS for Salmonella, and BMS and BMYS for Shigella than those of the other treatments, MS was, however, contaminated easier than US by pathogenic bacteria. From above results, synbiotics containing facultative anaerobic yeast have effects for preservation of TMR fermented anaerobically. Particularly, BMYS treatment having good results in nutrient contents, dry matter loss and pathogenic bacteria amounts was a resonable synbiotics for preservation of the fermented-TMR.
Anticancer Activity of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on MCF-7 Breast Cancer Cells
Kim, Ji-Eun ; Park, Joon-Hee ; Kang, Byoung-Won ; Seo, Min-Jeong ; Choi, Yung-Hyun ; Lim, Hak-Seob ; Seo, Kwon-Il ; Kim, Jeong-In ; Joo, Woo-Hong ; Lee, Bok-Kyu ; Jeong, Yong-Kee ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1435~1441
DOI : 10.5352/JLS.2008.18.10.1435
In this study, anti-cancer activities of peel of Citrus junos (CJP) and Poncirus trifoliata (PTP) on MCF-7 breast cancer cells, and anti-proliferative effects of cancer cells induced by environmental hormones were investigated. The ethanol extracts of CJP and PTP inhibited cancer cell growth and induced apoptosis at the concentration over 300 mg/ml treatment for 72 hr. Morphological change of MCF-7 breast cancer cells were observed treated with the CJP and PTP of 500 mg/ml concentration for 72 hr, and apoptosis was induced by activation of caspase-3. The proliferation of MCF-7 breast cancer cells was decreased in a dose-dependent manner treated with various concentration of CJP and PTP, when compared with the control at 300 mg/ml, the proliferation of the MCF-7 breast cancer cells of both extracts was decreased over 70% and 80%, respectively.
Genetic Relationship between Populations and Analysis of Genetic Structure in Hanwoo Proven and Regional Area Populations
Oh, Jae-Don ; Jeon, Gwang-Joo ; Lee, Hak-Kyo ; Cho, Byung-Wook ; Lee, Mi-Rang ; Kon, Hong-Sik ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1442~1446
DOI : 10.5352/JLS.2008.18.10.1442
Seven populations of 586 Hanwoo have been characterized by using 10 microsatellite DNA markers. Size of microsatellite markers decided using GeneMapper Software (v.4.0) after analyze in kinds of ABI machine of name of 3130. Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. Genetic distancesbetween populations were obtained using Ne's DA distance method. Expected heterozygosity between each population was estimated very analogously. Genetic distances (0.0413) between Kangwan (KW) and Gyonggi (GG), Jeonpuk (JP) were nearest than distances between other populations by 0.021. Genetic distances between Gyonggi (GG) and Kyongpuk (KP) showed far distance than other populations by 0.032. In the UPGMA tree that is made based on DA distance matrix. Each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all Hanwoo of each regional area populations. But Hanwoo proven population was ramified to different group.
Effect of Heat Processing on Thermal Stability of Kudzu (Pueraria thumbergiana Bentham) Root Isoflavones
Choi, Sung-Won ; Kim, Kyung-Seon ; Hur, Nam-Yun ; Kim, Kyung-Seon ; Ahn, Soon-Cheol ; Park, Cheon-Seok ; Kim, Byung-Yong ; Baik, Moo-Yeol ; Kim, Dae-Ok ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1447~1454
DOI : 10.5352/JLS.2008.18.10.1447
Effect of heat processing on thermal stability of kudzu root isoflavone was investigated for future use such as various processed foods and functional foods. Kudzu root extracts were heated at 80, 100, 121, 140, 165, and
for up to 90 minutes before and after concentration, respectively. Changes in the amount of isoflavones were monitored using HPLC and thermal stability was investigated using Arrhenius equation. The amount of both daidzin and genistin decreased slightly during heating at 80, 100 and
but decreased significantly above
. This indicated that daidzin and genistin are stable at temperatures near the boiling point of water. The degradation of both daidzin and genistin occurred in two steps and each step showed typical first order kinetic. The degradation rates were faster in the first step than the second step in both daidzin and genistin. Additionally, the degradation was accelerated when they heated after concentration compared to the sample heated before concentration. These results suggested that degradation of kudzu root isoflavone was highly dependent on both their concentration and heating temperature. This study provides the basic information on thermal stability of kudzu root isoflavones, which can be used for future processing of functional foods.
Effects of Different Exercise Intensity on Excess Post Exercise Oxygen Consumption (EPOC), Resting Metabolic Rate (RMR), and Biochemical Variables in Obese and NIDDM Patients
Kwak, Yi-Sub ; Ku, Woo-Young ; Yoo, Byung-In ; Jin, Young-Wan ; Choi, Kyung-Suk ; Cho, Joon-Yong ; Woo, Jin-Hee ; Hwang, Hye-Jin ;
Journal of Life Science, volume 18, issue 10, 2008, Pages 1455~1463
DOI : 10.5352/JLS.2008.18.10.1455
The purpose of this study was to evaluate the effect of various exercise intensity on Excess post exercise energy expenditure (EPEE), Resting Metabolic Rate (RMR),thyroid hormonal changes and biochemical variables in obese and NIDDM patients. The subject of the present study were divided into four groups and four periods: trained (T; n=10), control (C; n=10), obese (O; n=10) and NIDDM (N; n=10) group. And the periods were divided as follows; Resting (RE), Maximal (MA), High intensity (HI), and Low intensity (LI). There was significant difference in RMR among different intensity of exercise. in the T (p<0.05) not in the C, O, and N groups. however, there was no significant different percent body fat among all groups. In the energy expenditure, there was significant different among C, O, N groups compare to T in HIEE (high intensity exercise energy expenditure), LIEE (low intensity exercise energy expenditure), HIEEPE (high intensity exercise energy expenditure post exercise) and LIEEPE (low intensity exercise expenditure post exercise). In the hormonal level, there was significant different in T4 level in the T group at LI period and there was also significant difference in T4, Free T3, & Free T4 levels in T group at LI period, however there was no significant different in the O and N groups except LI period. In the fatigue variables, there was significant different in lactate and ammonia levels in the N group in the period of HI compare to C. The present cross-sectional study was design to investigate the relationship between exercise intensity and RMR in four groups. The focus of this investigation was to compare RMR in aerobically trained (T), control (C), obese (O) and NIDDM (N) group. The relationship among RMR, exercise intensity and percent body fat would best be investigated using Meta Lyzer 3B, MMX3B and body composition analyzer. Each subject completed measurement of percent body fat, RMR, hormone in the period of maximal oxygen uptake exercise (MA), high intensity exercise (HI), and low intensity exercise (LI). From the results, High and Low intensity of exercise, there was a trend for an increased RMR (kcal/day) in the trained groups and control group (in case of LI) not for the obese and N groups. This is best explained not by the reduced percent body fat but by the highly induced energy expenditure (during exercise and post exercise energy expenditure) and increased T4, Free T3, and Free T4 hormonal levels in the low intensity exercise for the T group and sometimes C group.