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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
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Alterations of Heart Rate Variability upon β3-Adrenergic Receptor Polymorphism and Combined Capsaicin, Sesamin, and L-Carnitine in Humans
Shin, Ki-Ok ; Kim, Hyun-Jun ; Kang, Sung-Hwun ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 291~297
DOI : 10.5352/JLS.2008.18.3.291
We investigated whether 1) the combined capsaicin (75 mg), sesamin (30 mg), and L-carnitine (900 mg) (CCSC) ingestion enhances autonomic nervous system (ANS) activities including thermogenic sympathetic activity as energy metabolic modulator, 2)
polymorphism of each subject influences with ANS activity. Seven healthy males
volunteered for this study. The cardiac autonomic nervous activities evaluated by means of heart rate variability of power spectral analysis were continuously measured during 5 min every 30 min for total 120 min resting condition with CCSC or placebo oral administration chosen at random. The results indicated that, there are not
variants of the
genotypes in our subjects. There were not also significant differences in heart rate during rest between both trials. The difference of ANS activity did not reach the statistical significance between both trials. However, the significant improvement showed TOTAL power, HF component, and the indices of SNS and PNS activities before and at 30 min after CCSC ingestion (p<0.05, respectively). In conclusions, although each component of combined CCSC is associated with lipolysis and/or fat oxidation, the combined CCSC consumption is not influenced in stimulation of thermogenic sympathetic activity as modulator of energy metabolism. In rather, our results suggested that CCSC ingestion improves the balance of both SNS and PNS activities. Therefore, it will be considered many combined nutrient components for ergogenic and/or lipolysis effects as well as genetic variants affecting ANS activity in further studies.
Identification of a Transferrin Receptor-binding Peptide from a Phage-displayed Peptide Library
Kim, Sung-Il ; Choi, Suk-Jung ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 298~303
DOI : 10.5352/JLS.2008.18.3.298
Using a phage peptide library approach, we have isolated a peptide ligand that binds to transferrin receptor on the surface of human melanoma cell, B16F10. The library was first screened twice by recovering internalized phages and was further screened three times by competitively eluting transferrin receptor-specific phages with human transferrin among the phages bound to the cell surface. The peptides displayed by the selected phages were fused to translocation and catalytic domain of Pseudomonas exotoxin to prepare recombinant toxins. After estimating cytotoxicity of each recombinant toxin toward B16F10 cell, seven clones were selected. Sequence analysis revealed that one of the clones displayed a peptide which had a significant sequence homology with human transferrin. The peptide was chemically synthesized and was shown to be functional in delivering cytotoxic agents into B16F10 cell via interaction with transferrin receptor.
Overexpression of NtHSP70-1 Protects Chlorophyll from High Temperature in Plants
Cho, Eun-Kyung ; Hong, Choo-Bong ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 304~310
DOI : 10.5352/JLS.2008.18.3.304
Heat shock protein 70 (HSP70) is known as molecular chaperone, the fundamental protein participating in various processes, from nascent protein synthesis to protection of proteins during abiotic stresses and developmental programs. However, their biological functions in plants are not yet well known. Here, NtHSP70-1 (AY372069), HSP70 of Nicotiana tabacum induced by heat stress was investigated. To analyze the protective role of NtHSP70-1, transgenic tobacco plants, which constitutively overexpressed NtHSP70-1 as well as contained either the vector alone or having NtHSP70-1 in the antisense orientation, were constructed. The altered NtHSP70-1 levels in plants were confirmed by western blotting and transgenic sense lines exhibited tolerance to heat stress. Seedlings with the constitutively expressed NtHSP70-1 grew as green or healthy plants after heat stress. In contrast, transgenic vector or antisense lines exhibited yellowing of leaves or some delay in growth, which finally led to death. Evaluation of chlorophyll contents of heat-shocked transgenic tobacco seedlings indicated that NtHSP70-1 contributes to thermotolerance by preventing chlorophyll synthesis in plants.
Suppression of Reactive Oxygen Species Production by Water-extracts of Coptidis Rhizoma Enhances Neuronal Survival in a Hypoxic Model of Cultured Rat Cortical Cells.
Choi, Ju-Li ; Shin, Gil-Jo ; Lee, Won-Chul ; Moon, Il-Soo ; Jung, Seung-Hyun ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 311~317
DOI : 10.5352/JLS.2008.18.3.311
Pathophysiological oxidative stress results in neuronal cell death mainly due to the generation reactive oxygen species (ROS). In low oxygen situation such as hypoxia and ischemia, excessive ROS is generated. Coptidis Rhizoma (CR) is a traditional medicine used for the incipient stroke. In this report we show that CR water extracts
exhibited protective effects of neuronal cell death in a hypoxic model (2%
3 hr) of cultured rat cortical cells. We further show that CR water extracts significantly reduced the intensity of green fluorescence after staining with
on one hour and three days after hypoxic shock and in normoxia as well. Our results indicate that CR water extracts prevent neuronal death by suppressing ROS generation.
System Design and Implementation for the Efficient Management and Automatic Update of Protein-Protein Interaction Data.
Kim, Ki-Bong ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 318~322
DOI : 10.5352/JLS.2008.18.3.318
This paper deals with an efficient management and automatic update sub-system for WASPIFA (Web-based Assistant System for Protein-protein Interaction and Function Analysis) system that had been developed in the past and now provides the comprehensive information on protein-protein interaction and protein function. Protein interacting data has increased exponentially, so that it costs enormous time and effort. In other words, it is actually impossible to manually update and manage an analysis system based on protein interacting data. Even though there exists a good analysis system, it could be useless if it was able to be updated timely and managed properly. Unfortunately, in most cases, biologists without professional knowledge on their analysis systems have to cope with a great difficulty in running them. In this respect, the efficient management and automatic update subsystem of protein interacting and its related data has been developed to facilitate experimental biologists as well as bioinformaticians to update and manage the WASPIFA system.
Antioxidant Effect of Oil Containing Cellulase-Treated Red Ginseng.
Kim, Hyun-Jeong ; Yang, Seun-Ah ; Im, Nam-Kyung ; Jhee, Kwang-Hwan ; Lee, In-Seon ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 323~328
DOI : 10.5352/JLS.2008.18.3.323
In this study we evaluated the method to develop red ginseng oil containing high content of phytochemicals by enzymes treatment. To select the optimum extraction process of red ginseng with oils, the antioxidant activities of red ginseng using various enzymes were measured. Red ginseng after 0.5% cellulase treatment for 1 hr at
had higher antioxidant activity than the other conditions. We found that red ginseng/soybean oil extracted for 15 days at
after 0.5% cellulase treatment increased DPPH radical scavenger activity and decreased the TBA and POV values. However, red ginseng/olive oil had little functional activities compare to the red ginseng/soybean 0il. We also analyzed vitamin A and E by HPLC and found that vitamin E was increased by 0.5% cellulase treatment in the oil. This is the first report that red ginseng oil extracted by enzyme treatment has various beneficial effects.
Study of Deep Ground Sea-Like Water on Antioxidant Activity and the Immune Response in RAW264.7 Macrophages.
Kim, Yu-Jung ; Jung, Il-Sun ; Song, Hyo-Ju ; Choi, Eun-Young ; Choi, In-Soon ; Choi, Young-Ju ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 329~335
DOI : 10.5352/JLS.2008.18.3.329
Korean Deep ground sea-like water (KDSW) has a similar mineral composition with deep sea water. KDSW has demonstrated its usefulness and attracted in the medical fields. KDSW and Danasoo (desalted deep ground sea-like water) intake improve antioxidant, antidiabetic activity and immunity. Antioxidant activities of KDSW and Dnansoo were measured by using 2,2-diphenyl-l-picryl-hydrazyl (DPPH) free radical, superoxide dismutase-like activity (SODA) and photochemiluminescence (PCL). DPPH radical scavenging and SOD-like activities of KDSW and Danasoo were remarkably increased in a dose-dependent manner. Antioxidant activities of KDSW and Danasoo 85.32 and 14.02 nmol of ascorbic acid equivalent/ml KDSW and Danasoo, respectively, using the PCL method. Lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophages RAW264.7 cells was inhibited up to 30% by treatment with Danasoo (20%). NO is synthesized by the enzyme of nitric oxide synthase (NOS) and plays an important role tumor growth and angiogenesis. The anticancer effects of Danasoo on human gastric and lung cancer cells was performed by levels of inducible nitric oxide synthase (iNOS). Danasoo significantly reduced iNOS expression of human gastric cancer (SNU-l) and lung carcinoma (A549). The serum glucose level was significantly reduced by Danasoo (20%) diet in streptozotocin (STZ)-induced diabetic rats. These result suggest that KDSW has excellent biological activities and thus it has great potential as a source for natural health products.
Induction of Apoptosis by HDAC Inhibitor Trichostatin A through Activation of Caspases and NF-κB in Human Prostate Epithelial Cells.
Park, Cheol ; Jin, Cheng-Yun ; Choi, Byung-Tae ; Lee, Won-Ho ; Choi, Yung-Hyun ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 336~343
DOI : 10.5352/JLS.2008.18.3.336
Histone deacetylases (HDACs) inhibitors have emerged as the accessory therapeutic agents for various human cancers, since they can block the activity of specific HDACs, restore the expression of some tumor suppressor genes and induce cell differentiation, cell cycle arrest and apoptosis in vitro and in vivo. In the present study, we investigated that the effect of trichostatin A (TSA), an HDAC inhibitor, on the cell growth and apoptosis, and its effect on the nuclear factor-kappaB
activity in 267B1 human prostate epithelial cells. Exposure of 267B1 cells to TSA resulted in growth inhibition and apoptosis induction in and dose-dependent manners as measured by fluorescence microscopy, agarose gel electrophoresis and flow cytometry analysis. TSA treatment inhibited the levels of IAP family members such as c-IAP-1 and c-IAP-2 and induced the proteolytic activation of caspase-3, -8 and -9, which were associated with concomitant degradation of poly (ADP-ribose)-polymerase,
and laminin B proteins. The increase in apoptosis by TSA was connected with the translocation of
from cytosol to nucleus, increase of the DNA binding as well as promoter activity of
, and degradation of cytosolic inhibitor of KappaB
protein. We therefore concluded that TSA demonstrated anti-proliferative and apoptosis-inducing effects on 267B1 cells in vitro, and that the activation of caspases and
may play important roles in its mechanism of action. Although further studies are needed, these findings provided important insights into the possible molecular mechanisms of the anti-cancer activity of TSA.
Comparison of Cellular Senescence Phenotype in Human Fibroblasts from New-born and Aged Donors.
Yi, Hye-Won ; Hwang, Eun-Seong ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 344~349
DOI : 10.5352/JLS.2008.18.3.344
Normal somatic cells proliferate for a limited number of doublings in culture and then enter an irreversible growth-arrest state called replicative senescence. Replicative senescence has been believed a reason for the limited cellular turnover and deterioration of tissue function in aged animals. However, there is no experimental evidence supporting this assumption. Furthermore, cells from aged person have been poorly characterized with an exception of the cases of T cells. In this study, we examined cell biological changes occurring in replicative senescence of fibroblast strains originated from a new-born (NHF-NB) and a 87 year old man (NHF-87). NHF-87 (and the cells from a 75-year old) proliferated to smaller population doublings and with longer doubling times than NHF-NB did. At early passages, NHF-87 exhibited a low senescence-associated
) activity and lipofuscin level, typical markers for cellular senescence. Furthermore, they maintained low levels of lysosome and reactive oxygen species (ROS). All of these levels increased dramatically in the late passage NHF-87 quite similarly as those in the late passaged NHF-NB did. These results indicate that most cells originated from the aged maintain a phenotype of the cells originated from new-born donors and undergo replicative senescence with the same kinetics as that of the cells from new-born. It is also indicated that not SA
activity but cell proliferation rate may be qualified as a biomarker for cells aged in vivo.
Biochemical Properties of Eggplant Fruit Lectin.
Roh, Kwang-Soo ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 350~356
DOI : 10.5352/JLS.2008.18.3.350
Biochemical characterization including hemagglutination of erythrocytes, molecular weight, optimum temperature, thermal stability, optimum pH, carbohydrate specificity, and inhibitory effect of metal ion were studied in lectin of eggplant (Solanum melongena L.) fruit prepared by ammonium sulfate fractionation and affinity chromatography. This lectin was agglutinated by trypsin-treated rat blood erythrocyte. The molecular weight of this lectin by SDS-PAGE was estimated to be approximately 19.3 kDa of a single band. This lectin has no activity by 7 carbohydrates containing D-glucose. The optimum range of temperature and pH were
and pH 6.2-7.2, respectively. This lectin was relatively stable at
. And the activity of this lectin was not inhibited by
Effects of Aralia continentalis Kitagawa on Antiplatelet and Antioxidative Activities.
Yang, Seun-Ah ; Im, Nam-Kyung ; Jhee, Kwang-Hwan ; Lee, In-Seon ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 357~362
DOI : 10.5352/JLS.2008.18.3.357
The dried roots of Aralia continentalis Kitagawa is known to have the potential for anti-inflammation and anti-rheumatic, but their effects on thrombosis are not clear. In this study, we evaluated the anti-platelet and antioxidative activities of A. continentalis Kitagawa. Methanol extract and its various fractions of A. continentalis Kitagawa inhibited ADP-induced platelet aggregation, and EtOAc fraction showed strongest inhibition in a concentration-dependent manner with a
value of 217.7
. Moreover, the EtOAc fraction contained 77.7% of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 43.1% of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity at 0.1 mg/ml. In addition, the methanol and EtOAc fraction dose-dependently inhibited thrombin-stimulated platelet adhesion to collagen or fibrinogen. Collectively, these results suggest that the polyphenol-rich EtOAc fraction from A. continentalis Kitagawa can reduce platelet hyperactivation by scavenging free radicals. Thus, the EtOAc fraction of A. continentalis Kitagawa is a potential source for inhibition of platelet-dependent thrombosis.
Screening of Thrombin Inhibitor and its DPPH Radical Scavenging Activity from Wild Insects.
Ryu, Hee-Young ; Heo, Jin-Cheol ; Hwang, Jae-Sam ; Kang, Seok-Woo ; Yun, Chi-Young ; Lee, Sang-Han ; Sohn, Ho-Yong ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 363~368
DOI : 10.5352/JLS.2008.18.3.363
The in vitro thrombin inhibitory activities of 304 crude extracts from 76 kinds of korean wild insects were evaluated. Measurement of thrombin time showed that the DMSO extracts of Acrida cinerea cinerea (Thunberg), Anax parthenope julius Brauer, Eurydema rugosa Motschulsky, and Stethophyma magister (Rehn) and the water extracts of Dolycoris baccarum Linne, Lixus divaricatus Motschulsky, Metrioptera bonneti, Moechotypa diphysis (Pascoe), Nicrophorus concolor sp., and Tomapoderus ruficollis (Fabricius) had strong thrombin inhibitory activity. No prominent changes of activated partial thromboplastin time by treatment of the selected extracts suggested direct inhibition of thrombin activity by the insect extracts. DPPH scavenging activity of selected extracts showed that the extract of A. cinerea cinerea (Thunberg), D. baccarum Linne, L. divaricatus Motschulsky and N. concolor sp. has good antioxidant activity as well as antithombin activity. Our results suggested that some of korean wild insects could be developed as a natural source of antithrombosis.
Antimicrobial Susceptibility Patterns of Staphylococcus intermedius Isolated from Skin Lesions of Thoroughbred Horse.
Choi, Seong-Kyoon ; Park, Cheong-Kyu ; Kwon, Oh-Deog ; Cho, Gil-Jae ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 369~373
DOI : 10.5352/JLS.2008.18.3.369
Staphylococcus intermedius (S. intermedius) has been rarely isolated from horse. In this study, we investigated the antimicrobial susceptibility patterns of S. intermedius isolated from skin lesions of Thoroughbred horse in Daegu Equestrian Association. The skin lesions were showed with dehairing and slight purulent inflammation. Bacteria were isolated from skin lesions and identified as S. intermedius by biochemical tests and MicroLog (BIOLOG, California, USA). Antimicrobial susceptibility test of S. intemedius isolates was performed with 33 antimicrobial agents (BBL, Maryland, USA) by using the agar disk diffusion method. It showed a high sensibility in the amikacin, amoxycillin/clavuramic acid, ampicillin, cefoxitin, cephalothin, chloramphenicol, ciprofloxacin, enrofloxacin, florfenicol, kanamycin, neomycin, nitrofurantoin, norfloxacin, ofloxacin, oxacillin, penicillin G, tetracycline and vancomycin. The horse was treated with penicillin, and cured completely after two weeks. The present results showed valuable information for treatment and prevention of skin disease in horse.
Comparison of the Real-Time Nucleic Acid Sequence-Based Amplification (NASBA) Assay, Reverse Transcription-PCR (RT-PCR) and Virus Isolation for the Detection of Enterovirus RNA.
Na, Young-Ran ; Joe, Hyeon-Cheol ; Lee, Young-Suk ; Bin, Jae-Hun ; Cheigh, Hong-Sik ; Min, Sang-Kee ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 374~380
DOI : 10.5352/JLS.2008.18.3.374
Rapid detection of enterovirus (EVs) is important in the management of aseptic meningitis. We examined the relative efficiency and specificity of the real-time nucleic acid sequence-based amplification (NASBA) comparing with the established reverse transcription polymerase chain reaction (RT-PCR) and viral culture method which were used for the detection of enterovirus RNA in clinical specimens. Of the total 292 samples, 145 were found to be positive to enterovirus RNA by real-time NASBA, 101 were positive by viral culture, and 86 were positive by RT-PCR. 147 samples and 46 samples were determined to be negative and positive by all methods respectively, but 4 samples were positive only by real-time NASBA. To compare the specificity of each method, various clinical samples which were diagnosed for herpes simplex virus (HSV)-1, HSV-2, adenovirus, mumps, and rhinovirus were applied. Except one rhinovirus sample which was false positive to enterovirus RNA by RT-PCR, the other different samples were negative to all three methods. The real-time NASBA procedure can be completed within 5 hours in contrast with 9 hours for the RT-PCR and 3-14 days for the viral culture. From this study, it was suggested that the real-time NASBA assay could be a standardized, rapid, specific, and sensitive procedure for the detection of enterovirus RNA.
Effect of Deep Sea Water on Phase I, Phase II and Ornithine Decarboxylase.
Shon, Yun-Hee ; Kim, Mee-Kyung ; Jang, Jung-Sun ; Jung, Eun-Jung ; Nam, Kyung-Soo ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 381~386
DOI : 10.5352/JLS.2008.18.3.381
Deep sea water was tested for cancer chemopreventive activity by measuring the activities of
cytochrome P 450 1A2 (CYP 1A2), quinone reductase (QR) and glutathione-S-transferase (GST), glutathione (GSH) levels, and ornithine decarboxylase (ODC) activity. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of
) showed a hardness-dependent inhibition of CYP 1A2 activity. QR and GST activities were induced about
fold with the treatment of deep sea water in murine hepatoma Hepa 1clc7 cells. In addition GSH levels were increased
fold in a hardness range of
. The deep sea water showed 20.3 and 35.0% inhibition of 12-O- tetradecanoylphorbol-13-a-cetate (TPA)-induced ODC activity at hardness 800 and 1,000, respectively. Therefore, deep sea water is worth further investigation with respect to cancer chemoprevention or therapy.
Nutritional Composition and Biological Activities of the Methanol Extracts of Sea Mustard (Undaria pinnatifida) in Market.
Choi, Jae-Suk ; Bae, Hee-Jung ; Kim, Yang-Chun ; Park, Nam-Hee ; Kim, Tae-Bong ; Choi, Young-Ju ; Choi, Eun-Young ; Park, Sun-Mee ; Choi, In-Soon ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 387~394
DOI : 10.5352/JLS.2008.18.3.387
This research was performed to determine the proximate compositions, mineral contents, alginic acid, antioxidative activities and amino acids of sea mustards (Miyeok: Undaria pinnatifida) collected from Gijang and Wando area. Ash content was higher in Gijang samples, whereas carbohydrate and moisture were higher in Wando Sil Miyeok. General compositions of dried sea mustard showed different contents as manufacture's company and places. The major free amino acids were hydroxyproline, alanine, glutamic acid and asparagine in Gijang samples. Both Gijang and Wando Sil Miyeok showed lower contents comparing with Gijang Gadak Miyeok. Major mineral content was Na, K, Ca, Mg and P, and especially, Na and K were the most abundant in both Gijang and Wando sea mustards. Alginic acid content was almost similar in both sea mustards. Antioxidative activity of methanol extract of sea mustards was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging activity was 45.5%
in Gijang Gadak Miyeok and 37.0% and 26.0%
in Gijang and Wando Sil Miyok, respectively. SOD-like activity of Gijang and Wando Sil Miyok was 63% and 71%
, respectively. These results show that biological activities depend on Miyeok manufacture's process. When stimulated macrophages RAW264.7 cells with lipopolysaccharide (LPS), inhibition of NO production in Gijang Sil Miyeok (44.2%) was 9% high comparing with that of Wando Sil Miyeok (35.7%).
Investigation of Microbial Contamination the Level in Fish Farms of Jeju East Coast.
Kim, Yun-Beom ; Moon, Young-Gun ; Ha, Jin-Hwan ; Kang, Chang-Hee ; Kam, Sang-Kyu ; Song, Chun-Bok ; Oh, Myung-Cheol ; Heo, Moon-Soo ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 395~402
DOI : 10.5352/JLS.2008.18.3.395
Level of microbial contamination was examined in four fish farms of Jeju east coast for sanitary indication of bacterial contamination such as heterotrophic bacteria, Escherichia coli O157:H7, Salmonella spp., Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus cereus and total coliforms. The samples were collected and investigated from June to October in 2007. Bacterial species of S. aureus, B. cereus and Salmonella spp. were frequently detected from the seawater of above fish farms. Salmonella spp., S. aureus, B. cereus were detected in the range of
CFU/ml, respectively, from the fish feed used in all four selected farms. Additionally, Salmonella spp., S. aureus, B. cereus were detected in oliver flounder (Paralichthys olivaceus) of these farms, however total coliforms and E. coli O157:H7 were not detected during our experimental period. For the production of microbiologically safe and healthy oliver flounder, proper quality control of feed, sanitation programs, and continuous monitoring of microorganism are essential practices, which required to include in the farm management system.
Effect of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on Antioxidant and Immune Activity.
Park, Joon-Hee ; Kang, Byoung-Won ; Kim, Ji-Eun ; Seo, Min-Jeong ; Lee, Young-Choon ; Lee, Jai-Heon ; Joo, Woo-Hong ; Choi, Yung-Hyun ; Lim, Hak-Seob ; Jeong, Yong-Kee ; Lee, Bok-Kyu ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 403~408
DOI : 10.5352/JLS.2008.18.3.403
In this study, we compared with 80% ethanol extracts from peel of Poncirus trifoliata (PTP) and peel of Citrus junos (CJP) against antioxidant and immune activities. Total phenolics and flavonoids contents in PTP extracts were
mg/l00 g, respectively, and those were lower than CJP extracts. Antioxidant activities of PTP were increased with the more concentration, and were similar to CJP. Antioxidant activities of PTP were increased with increasing of concentration, and were similar to those of CJP. The NO production in macrophage cell lines were increased in a dose-dependent manner, until 5 mg/ml of CJP and 1 mg/ml of PTP compared with control cells, but decreased at higher concentrations. The proliferation of mouse spleen cells were increased in a dose-dependent manner, until 1 mg/ml of CJP and PTP compared with control cells but decreased at higher concentrations. The NO production in macrophage cell lines treated with PTP and CJP were increased in a dose-dependent manner, compared with untreated control cells until the concentrations of
mg/ml (CJP) and 1 mg/ml (PTP) but decreased at higher concentrations than that. The proliferation of mouse spleen cells treated with PTP and CJP were increased in a dose-dependent manner, compared with untreated control cells until the concentration of 1 mg/ml but decreased at higher concentrations than that.
Comparison of Arylphorin of Antheraea pernyi with Those of Several Lepidopteran Wild Silkmoths by Western Blot Analysis.
Park, Nam-Sook ; Kim, Mi-Ae ; Park, Hyun-Chul ; Kim, Keun-Ki ; Jin, Byung-Rae ; Lee, Sang-Mong ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 409~413
DOI : 10.5352/JLS.2008.18.3.409
The occurences of proteins relating to Antheraea pernyi arylphorin in haemolymph, fat body, integument, midgut and silkgland of the wild silkmoths, Antheraea yamamai, Antheraea pernyi, Samia cynthia pryeri and Actias gnoma in the 5th larval instar were investigated by immunoblot analysis using mouse polyclonal antibody against A. pernyi arylphorin as probe. In A. yamamai, A. pernyi, S. cynthia pryeri and A. gnoma, the major immunoreactive antigenic proteins with a molecular weight of 80 KDa against the antisera of the A. pernyi arylphorin were clearly observed in the haemolymph, but in the integument, fat body, midgut and silkgland of the corresponding wild silkmoths the presence of the immunoreactive proteins were very variable. These results suggest that the A. pernyi arylphorin has almost same immunological identity with those of the wild silkmoths, A. yamamai, S. cynthia pryeri and A. gnoma though the distribution of the corresponding antigenic arylphorins is different according to the tissues of the wild silkmoths.
Adenocarcinoma of Meibomian Glands and Moll Glands in the Eyelid of Canine
Yuan, Dong-wei ; Goo, Moon-Jung ; Yang, Hai-jie ; Hong, Il-Hwa ; Ki, Mi-Ran ; Park, Jin-Kyu ; Lee, Hye-Rim ; Yoo, Sung-Eun ; Jeong, Kyu-Shik ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 414~417
DOI : 10.5352/JLS.2008.18.3.414
A 4-year-old female beagle with progressive exophthalmos and which had a neoplastic mass with diameter of 1.4 cm in the left lower ocular adnexa. Histologically, the mass was composed of hyper-plastic lobules and tubular structures separated by fibrous septum. The well differentiated sebaceous gland forming various sized lobules, and infiltration of mast cells and mononuclear inflammatory cells were observed. Apical decapitation secretion of these tubular structures with basophilic materials in their lumen showed mild sebaceous gland metaplasia. Immunohistochemical studying, cell groups were positive in
and vimentin. The primary tumor was diagnosed as adenocarcinoma originated from moll gland and meibomian gland of the eyelid, and the infiltrating intraocular neoplasm was diagnosed as a malignant mixed tumor.
Properties of the Membrane-Bound NADH;Menadione Oxidoreductase in the Aerobic Respiratory Chain of Bacillus cereus
Kang, Ji-Won ; Kim, Young-Jae ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 418~421
DOI : 10.5352/JLS.2008.18.3.418
Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linked NADH oxidase system exhibited an apparent
value of approximately 65
for NADH. On the other hand, the enzymatic properties of the NADH: menadione oxidoreductase of NADH oxidase system were examined. The maximum activity of NADH: menadione oxidoreductase was obtained at pH 9.5 in the presence of 0.1 M KCl (or NaCl). The NADH: menadione oxidoreductase activity was very resistant to the respiratory chain inhibitors such as rotenone, capsaicin, and
. Interestingly, the activity was stimulated by the 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO).
Activities of Recombinant MT1-MMP Expressed in PANC-1 Cells.
Kim, Hye-Nan ; Chung, Hye-Shin ;
Journal of Life Science, volume 18, issue 3, 2008, Pages 422~425
DOI : 10.5352/JLS.2008.18.3.422
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a membrane-associated zinc-dependent endoproteinase involved in extracellular matrix remodeling. MT1-MMP hydrolyzes ECM proteins like collagen and is involved in cancer cell migration and metastasis. Caveolins are integral membrane proteins and play a role in formation of caveolae, specialized membrane microdomains involved in clathrin-independent endocytosis. Recombinant MT1-MMP was transiently expressed in PANC-1 cells. Cells expressing recombinant MT1-MMP were able to hydrolyze collagen and migrate on collagen coated trans-well. Both subjacent collagen degradation and the cell migration conferred by recombinant MT1-MMP were inhibited by co-transfection of plasmids containing caveolin-1 cDNA. The results support that MT1-MMP is localized in lipid raft of the membrane and MT1-MMP activities in invasive cells could be inhibited by caveolin.