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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
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Polymorphisms of 3-hydroxy-3-methylglutaryl Coenzyme A Reductase Gene Are Not Associated with the Osteonecrosis of Femoral Head in Korean
Kim, Tae-Ho ; Hong, Jung-Min ; Lee, Sang-Han ; Park, Eui-Kyun ; Kim, Shin-Yoon ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 427~434
DOI : 10.5352/JLS.2008.18.4.427
Osteonecrosis of the femoral head (ONFH) is a multifactorial disease and certain individuals are more at risk or may be predisposed to it. An altered lipid metabolism is one of the major risk factors for osteonecrosis, especially corticosteroid therapy and alcoholism. 3-hydroxy-3-methylglutaryl coenzyme A. (HMG-CoA) reductase inhibitors, stalin used as lipid-clearing agent, have been known to decrease the risk of osteonecrosis in patients receiving steroids and affect coagulation and fibrinolysis. Therefore we evaluated the association of HMG-CoA reductase gene polymorphisms and haplotypes between osteonecrosis patients and normal controls. We directly sequenced the HMG-CoA reductase gene in 24 Korean individuals, and identified five sequence variants. Four SNPs (-6933C>T, -6045T>G, +12673G>A, and +18128C>T) were selected and genotyped in 349 male ONFH patients and 300 male control subjects. The genotypes, allele frequencies, and haplotypes of the polymorphisms in the total patients as well as in the subgroup by etiology were not significantly different from those in the control group. In addition, no significant differences between each genotype of the polymorphisms and plasma lipid level could be found in the control group. These results suggest that the polymorphisms and haplotypes of HMG-CoA reductase gene are unlikely to be associated with a susceptibility to ONFH.
Genetic Diversity of Rehmannia glutinosa Genotypes Assessed by Molecular Markers
Bang, Kyong-Hwan ; Chung, Jong-Wook ; Kim, Young-Chang ; Lee, Jei-Wan ; Kim, Hong-Sig ; Kim, Dong-Hwi ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 435~440
DOI : 10.5352/JLS.2008.18.4.435
Random amplified polymorphic DNA (RAPD) markers were used to identify the genetic diversities among and within varieties and landraces of Rehmannia glutinosa. Polymorphic and reproducible bands were produced by 10 primers out of total 20 primers used in the experiment. In RAPD analysis of the 11 genotypes, 64 fragments out of 73 amplified genomic DNA fragments were polymorphic which represented an average 6.4 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPA-1) to 13 (OPA-11) and varied in size from 200 bp to 1,400 bp. Especially, OPA-10, OPA-11 and OPA-19 primers showed specific bands for varieties of Korea Jiwhang and Jiwhang il ho, which could be useful for discriminating from other varieties and landraces of R. glutinosa. Percentage polymorphism ranged from a minimum of 50% (OPA-1) to a maximum of 100% (OPA-11), with an average of 87.7%. Similarity coefficients were higher in the genotypes of Korea Jiwhang and Jiwhang il ho than in other populations. In cluster analysis, genotypes of Korea Jiwhang, Jiwhang il ho, and Japanese accession were separated from those of other varieties and landraces. Average of genetic diversity within the population
was 0.110, while average of total genetic diversity
was 0.229. Across all RAPD makers the
value was 0.517, indicating that about 52% of the total genetic variation could be explained by RAPDs differences while the remaining 48% might be attributable to differences among samples. Consequently, RAPD analysis was useful method to discriminate different populations such as domestic varieties and other landraces. The results of the present study will be used to understand the population and evolutionary genetics of R. gllutinosa.
Effects of Tomato Extracts on Detergent-Induced Dry Skin in Rats
Na, Hyun-Sook ; Kim, Jong-Bong ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 441~446
DOI : 10.5352/JLS.2008.18.4.441
Dry skin, called xerosis as medical term, is one of the most common skin problems. Many epidemiological studies show that the consumption of foods containing lycopene plays an important role in protecting the epithelial tissue. In this study, water extracts of tomato (WET) containing lycopene were fed and applied to evaluate the effects on dry skin induced by kitchen detergent in rats. These effects were identified by protein analysis and histological changes such as inflammatory erythematic skin as well as acanthosis. The visual scoring for skin observation showed the value such as 4 indicating fiery red with edema after detergent application to skin for 3 wk. However, WET feeding and application to skin showed the decreased values, from 0.7 to 1.0. In addition, it was noteworthy that the epidermis of dry skin show apparent acanthosis with abnormally accentuated keratinization and parakeratosis. However, acanthosis was reversed by feeding and application of WET to dry skin. In order to analyze the effects of WET on dry skin induced by detergent, protein analysis was carried out. The increased amount of protein in dry skin after WET feeding and application would be suggested as one of biochemical mechanisms for recovering the damaged skin. Thus, it would be recommended that water-extracted tomato is a new ingredient in skin regeneration from dry skin induced by detergent.
Isolation and Identification of Zinc-Enriched Yeast Saccharomyces cerevisiae FF-10 from the Tropical Fruit Rambutan
Cha, Jae-Young ; Heo, Jin-Sun ; Kim, Jung-Wook ; Lee, Seon-Woo ; Cho, Young-Su ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 447~453
DOI : 10.5352/JLS.2008.18.4.447
Zinc is an essential trace element in Human Being. Highly zinc containing yeast strain isolated from the tropical fruit, rambutan and the zinc concentration in this yeast strain was 306 ppm (30.6 mg%)per dry matter basis. This strain was found to be a rounded type, normal size, and multi-polar budding. Phylogenetic analysis using the ITS1-5.85 rDNA sequences from isolated strain is most similar to yeast Saccharomyces cerevisiae at the level of nucleotide sequence identity at 99%. This strain was produced alcohol by about 12% using fully colonized koji-rice with Aspergillus oryzae. In conclusion, the isolated strain was found to be closely related to the S. cerevisiae based on its morphological and physiological properties, and alcohol fermentation. The phylogenetic analysis of strain FF-10 using ITS 5.8S rDNA sequence data also supported the closely related to the S. cerevisiae. Accordingly, the isolated yeast was named as S. cerevisiae FF-10. Further studies on the best culture conditions for zinc production from zinc-enriched S. cerevisiae FF-10 are under investigation.
Morphological, Cytological and Molecular Evidence for Intersubgeneric F
Hybrid between Glycine max x G. tomentella
Choi, In-Soo ; Kim, Yong-Chul ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 454~460
DOI : 10.5352/JLS.2008.18.4.454
This study was carried out to demonstrate morphological, cytological and molecular evidence for intersubgeneric
hybrid between Glycine tomentella and G. max cv. ‘Baemkong’. Morphological features of
plant for pistil and stamen, flower color and growth habit showed intermediate type between G. tomentella and G. max cv. ‘Baemkong’. Chromosome number of
plant was 2n=39, which explained the evidence of
hybrid between G. tomentella (2n=38) and G. max cv. ‘Baemkong’ (2n=40). Polyacrylamide isoelectric focusing pattern for esterase and peroxidase also showed that the
plant was true
hybrid between G. tomentella and G. max cv. ‘Baemkong’. From RAPD analysis, we identified that 62 primers showing bands in
hybrid had both bands from G. tomentella and G. max cv. ‘Baemkong’, which suggested that this was true
hybrid. Based on our results from morphological, cytological and molecular analyses, we suggest that the
plant was true intersubgeneric hybrid between G. tomentella and G. max cv. ‘Baemkong’. Our results still remain us further study to recover fertility of
hybrids. The occurrence of maternal and/or paternal inheritance in
hybrid from intersubgeneric cross between G. tomentella and G. max cv. ‘Baemkong’ need to be explained.
Smad-dependent Expression of Gadd45b Gene during TGF-β-induced Apoptosis in EpH4 Cells.
Cho, Hee-Jun ; Yoo, Ji-Yun ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 461~466
DOI : 10.5352/JLS.2008.18.4.461
)-dependent apoptosis is important in the elimination of damaged or abnormal cells from normal tissues in vivo. Gadd45b has been known to participate in
apoptosis by the activation of p38 kinase. In this report, we show that Gadd45b is an immediate-early response gene for
during apoptosis in EpH4 cells. To elucidate the molecular mechanism of
Gadd45b gene expression, we cloned the 5'-flanking region of the mouse Gadd45b gene. When transfected into EpH4 cells, this 5'-flanking region conferred promoter activity and inducibility by
. Deletion analyses demonstrated that the minimal promoter activity was detected in the proximal region 220 bp upstream of the transcription initiation site. We also found that the proximal Gadd45b promoter is activated by
through the action of Smad2, Smad3, and Smad4. Finally, we show that the expression of Gadd45b gene by
is suppressed in EpRas cells in which
could not induce apoptosis, suggesting that Gadd45b may be a crucial target for
apoptosis in EpH4 cells.
Antioxidative Effect of Pimpinella brachycarpa Ethanol Extract.
Lee, Yu-Mi ; Lee, Jae-Joon ; Lee, Myung-Yul ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 467~473
DOI : 10.5352/JLS.2008.18.4.467
This study was performed to investigate the antioxidant effect of 80% ethanol extracts from Pimpinella brachycarpa in vitro. The extraction yields of 80% ethanol extract was 9.23 g/100 g. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, n-butanol and water. Water fraction showed the highest extraction yield among fractions. Antioxidative activities of different fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical generation, Rancimat test, thiobarbituric acid (TBA) value, nitrite scavenging activity, inhibition of lipid peroxidation and peroxide value in linoleic acid in comparison with the commercial antioxidant butylated hydroxytoluene (BHT). Antioxidant activities of n-hexane fraction of Pimpinella brachycarpa ethanol extract were the highest among fractions. Furthermore, the antioxidative capacity of the n-hexane fraction was similar to that of BHT.
Mineral Contents and Physiological Activities of Dried Sea Tangle (Laminaria japonica) Collected from Gijang and Wando in Korea.
Choi, Jae-Suk ; Shin, Su-Hwa ; Ha, Yu-Mi ; Kim, Yang-Chun ; Kim, Tae-Bong ; Park, Sun-Mee ; Choi, In-Soon ; Song, Hyo-Ju ; Choi, Young-Ju ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 474~481
DOI : 10.5352/JLS.2008.18.4.474
This research was performed to determine the proximate compositions, mineral contents, alginic acid, antioxidative activities and amino acids of sea tangles collected from Gijang and Wando area. Crude protein and ash contents were higher in Gijang sea tangle, whereas carbohydrate and moisture were higher in Wando in general. Mineral contents of Gijang sea tangle were higher than Wando. Especially, Na and K was the most abundant in both Gijang and Wando sea tangles. Alginic acid content was almost similar in both sea tangles. The major free amino acids were glutamic acid, aspartic acid, alanine, proline and hydroxyproline in both Gijang and Wando sea tangles. Antioxidative activity of methanol extract of sea tangle was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activity were about 17% (
) and 7% (
) higher, respectively, in Wando sea tangle. When stimulate the macrophages RAW264.7 cells with lipopolysaccharide (LPS), inhibition of NO synthesis of the methanol extract was 11% higher in Wando sea tangle comparing with Gijang samples.
Screening and Identification of Alkaline Protease Inhibitor-Producing Marine-derived Actinomyces.
Kang, Sung-Il ; Kong, Jai-Yul ; Choi, Yeung-Joon ; Kim, Min-Yong ; Son, Hong-Joo ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 482~487
DOI : 10.5352/JLS.2008.18.4.482
In this study, we screened and identified the bacterial strain showing high alkaline pretense inhibitor activity from marine environment. Nine bacterial strains with alkaline pretense inhibitor activity were isolated from marine sediments. Among them, strain C12 had the highest alkaline pretense inhibitor activity and was selected for further taxonomical study. On the basis of morphological and physiological characteristics, strain C12 was identified as the genus Streptomyces. A phylogenetic analysis of the 165 rDNA showed that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces thermocarboxydus. Morphological characteristics showed cylindrical spore chain and smooth spore surface by scanning electron microscope. Strain C12 was grown on all media except for ISP 9 agar. This strain could be grown in the medium containing up to 9% NaCl.
Functional Analysis of the High Affinity Phosphate Transporter Genes Derived from Oryza sativa in Arabidopsis thaliana.
Seo, Hyoun-Mi ; Jung, Yun-Hui ; Kim, Yun-Hye ; Kwon, Tack-Min ; Jeong, Soon-Jae ; Yi, Young-Byung ; Kim, Doh-Hoon ; Nam, Jae-Sung ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 488~493
DOI : 10.5352/JLS.2008.18.4.488
Phosphate, a favorable phosphorous form for plant, is one of major nutrient elements for growth and development in plants. Plants exhibit various physiological and biochemical responses in reaction to phosphate starvation in order to maintain phosphate homeostasis. Of them, expression of high affinity phosphate transporter gene family and efficient uptake of phosphate via them is a major physiological process for adaption to phosphate deficient environment. Although the various genetic resources of high affinity phosphate transporter are identified recently, little is known about their functions in plant that is prerequisite information before applying to crop plants to generate valuable transgenic plants. We demonstrated that Arabidopsis transgenic plants over-expressing two different high affinity phosphate transporter gens, OsPT1 and OsPT7, derived from rice, exhibit better growth responses compared with wild-type under phosphate starvation condition. Specially, OsPT7 gene has proven to be more effective to generate Arabidopsis transgenic plant tolerant to phosphate deficiency than OsPT1. Furthermore, the expression level of AtPT1 gene that is one of reporter genes specifically induced by phosphate starvation was significantly low compared with wild-type during phosphate starvation. Taken together, these results collectively suggest that over expression of OsPTl and OsPT7 genes derived from monocotyledonous plant function efficiently in the dicotyledonous plant, relieving stress response caused by phosphate starvation and leading to better growth rate.
Immunotoxicity Study of Separated Antigen from Helicobacter pylori.
Park, Chang-Ho ; Bae, Man-Jong ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 494~502
DOI : 10.5352/JLS.2008.18.4.494
The anaphylaxis shock reaction on the whole cells of H. pylori exhibited a symptom of slight illness for the first and second medication of causing antigen at an antigen concentration of WC (H)
for WC (H) and no anaphylaxis shock symptom was observed at an antigen concentration of
for WC (L). In the case of anaphylaxis shock reaction on the crude urease, no symptom was observed at an antigen concentration of
for both urease (L) and urease (H). In the heterologous passive cutaneous anaphylaxis (PCA) test using a guinea pig-rat, no positive reaction was detected in all the medication groups of WC (H), WC (L), urease (H) and urease (L). In the skin sensitization test, it was observed that the best antigen concentration not causing skin disorder at each of
Effect of Deep Sea Water on Cytochrome P450 1A1, Aromatase and MMP-9.
Shon, Yun-Hee ; Kim, Mee-Kyung ; Nam, Kyung-Soo ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 503~508
DOI : 10.5352/JLS.2008.18.4.503
Deep sea water from the East sea was tested for breast cancer chemoprevention and metastasis by measuring the activities of cytochrome P450 1A1 and aromatase, invasiveness, and activity and expression of matrix metalloproteinase (MMP)-9 in breast MDA-MB-231 cancer cell. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of
) showed a hardness-dependent inhibition of 7,12-dimethylbenz[a]anthracene (DMBA)-induced cytochrome P450 1A1 activity. Deep sea water showed 27.1, 45.4 and 51.9% inhibition of microsomal aromatase activity at the hardness of 600, 800 and 1,000, respectively. In addition deep sea water inhibited not only the invasiveness of 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MDA-MB-231 cells through matrigel-coated membrane in a hardness-dependent manner but also the activity and expression of MMP-9 in MDA-MB-231 cell.
Effects of Mole Crickets (Gryllotalpa orientalis) Extracts on Anti-oxidant and Anti-inflammatory Activities.
Heo, Jin-Chul ; Lee, Dong-Yeob ; Son, Min-Sik ; Yun, Chi-Young ; Hwang, Jae-Sam ; Kang, Seok-Woo ; Kim, Tae-Ho ; Lee, Sang-Han ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 509~514
DOI : 10.5352/JLS.2008.18.4.509
Tremendous natural product extracts were used as a herb medicine remedy or therapy for centuries. Because these extracts have various biological activities, we examined the effects of Gryllotalpa orientalis extract for anti-oxidant and anti-inflammation activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), hydroxy radical scarvenging and cyclooxygenase-2 promoter assays. Gryllotalpa orientalis extracts were prepared from solvents such as distilled water (DW), dimethyl sulfoxide (DMSO), ethanol and methanol. The results showed that Gryllotalpa orientalis extracts have potent DPPH (methanol extract), FRAP (DW extract) and hydroxy radical scavenging (DW and methanol extracts) activity than any other extracts used. A significant inhibition of cyclooxygenase-2 (Cox-2) promoter activity was detected in the presence of DMSO extract or ethanol extract. Collectively, the present results suggested that Gryllotalpa orientalis extract could be used for anti-oxidant and/or anti-inflammation agent for human or agricultural purposes.
Effects of Non-ionic or Zwitterionic Surfactant on in vitro Digestibility of Rice Straw and Growth of Rumen Mixed Microorganisms.
Lee, Shin-Ja ; Kim, Wan-Young ; Moon, Yea-Hwang ; Kim, Hyeon-Shup ; Kim, Kyoung-Hoon ; Ha, Jong-Kyu ; Lee, Sung-Sill ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 515~521
DOI : 10.5352/JLS.2008.18.4.515
This experiment was conducted to investigate effects of non-ionic or zwitterionic (+/-) surfactants on digestibility of rice straw, and changes of growth of rumen mixed microbes, pH, and gas production during in vitro fermentation. Also, during in vitro ruminal fermentation, microbial attachment on rice straw was investigated using scanning electron microscopy (SEM). Tween 80 or SOLFA-850 for non-ionic surfactant (NIS), and 3-(Dodecyldimethylammonio) propanesulfanate (DDAP) for zwitterionic surfactant (ZIS) was supplemented by 0.05% and 0.1% in Dehority's artificial medium containing Holtein rumen fluid, respectively, and the substrate for fermentation was rice straw passed through 1 mm screen. The experiment was composed of 7 treatments (two levels of two NISs, two levels of a ZIS) including the control, and 6, 12, 24, 48 and 72 hr of fermentation time with 3 replications per treatment. Treatment of Tween 80 increased in vitro DM digestibilities during 48 hr and 72 h post fermentations compared to the other treatments, whereas treatment of DDAP as a ZIS resulted in decreased DM digestibility than that of the control from 24 hr post fermentation (P<0.05). Gas production in vitro was greater (P<0.05) with addition of NIS than the control or ZIS, and increased as fermentation time elapsed. Rumen mixed microbial growth was greatest with addition of Tween 80 as NIS, and lowest when DDAP as ZIS was supplemented to the fermentation tube (P<0.05). In SEM observation, rumen microbial population attached on rice straw particle was greater with addition of NIS, but was less with addition ZIS compared with the control. In conclusion we could not found any positive effects of ZIS surfactants on rumunal fermentation characteristics and rumen microbial growth rates.
Characterization of HtrA2-deficient Mouse Embryonic Fibroblast Cells Based on Morphology and Analysis of their Sensitivity in Response to Cell Death Stimuli.
Lee, Sang-Kyu ; Nam, Min-Kyung ; Kim, Goo-Young ; Rhim, Hyang-Shuk ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 522~529
DOI : 10.5352/JLS.2008.18.4.522
High-temperature requirement A2(HtrA2) has been known as a human homologue of bacterial HtrA that has a molecular chaperone function. HtrA2 is mitochondrial serine protease that plays a significant role in regulating the apoptosis; however, the physiological function of HtrA2 still remains elusive. To establish experimental system for the investigation of new insights into the function of HtrA2 in mammalian cells, we first obtained
MEF cells lines and identified those cells based on the expression pattern and subcellular localization of HtrA2, using immunoblot and biochemical assays. Additionally, we observed that the morphological characteristics of
MEF cells are different form those of
MEF cells, showing a rounded shape instead of a typical fibroblast-like shape. Growth rate of
MEF cells was also 1.4-fold higher than that of
MEF cells at 36 hours. Furthermore, we verified both MEF cell lines induced caspsase-dependent cell death in response to apoptotic stimuli such as heat shock, staurosporine, and rotenone. The relationship between HtrA2 and heat shock-induced cell death is the first demonstration of the research field of HtrA2. Our study suggests that those MEF cell lines are suitable reagents to further investigate the molecular mechanism by which HtrA2 regulates the balance between cell death and survival.
Effects of Pseudomonas fluorescens on Production of Several Inflammatory Mediators in the Human Alveolar Epithelial Cells.
Yang, Hyun ; Ryoo, Jung-Min ; Park, Seung-Hwan ; Choi, Hye-Jin ; Kim, Na-Yeon ; Cho, Hyung-Hoon ; Ahn, Jung-Hoon ; Moon, Yu-Seok ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 530~536
DOI : 10.5352/JLS.2008.18.4.530
To investigate the molecular mechanism of the airway inflammation by Pseudomonas fluorescens, effects on the inflammatory mediators such as interleukin-8 (IL-8), cyclooxygenase-2 (COX-2), macophage inhibitory cytokine 1 (MIC-1) were assessed in the human alveolar epithelial cells. Exposure to P. fluorescens and its recombinant bacteria suppressed cellular viability in the A549 epithelial cells and pro-inflammatory cytokine interleukin-8 production. However, pro-inflammatory prostaglandin-producing COX-2 protein was not altered by P. fluorescens though its mRNA was slightly elevated. As the inhibitory cytokine for the pro-inflammatory mediators, MIC-1 expression was monitored in A549 cells. MIC-1 gene induction was not significantly enhanced but the protein processing was changed by exposure to P. fluorescens. Pro-protein form of MIC-1 (
) was cleaved into active form mature MIC-1 (
) and propeptide (
) by the bacteria exposure. MIC-1 activation can contribute to the suppression of cellular viability by P. fluorescens and can retard IL-8-induced monocyte recruitment. However, sustained activation of MIC-1 can mediate the tissue injury by P. fluorescens exposure.
The Effect of Haptoglobin on Expression of Inflammatory Cytokines in 3T3-L1 Preadipocytes.
Cho, Jin-Kyung ; Kim, Nam-Hoon ; Oh, Mi-Kyung ; Park, Seon-Joo ; Kim, In-Sook ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 537~541
DOI : 10.5352/JLS.2008.18.4.537
White adipose tissue is now recognized as an important endocrine organ which secretes various signal factors and proteins termed 'adipokine'. Haptoglobin (Hp), which has been known as an acute phase protein, belongs to the adipokine. However, the function of Hp in adipose tissue remains unclear. To verify the role of Hp in preadipocytes, in this study, 3T3-L1 preadipocyte cells were stably transfected with human Hp gene and Hp-overexpressing cells were made. The Hp had no effect on cell growth of preadipocytes. By RT-PCR and Western blot analysis, the Hp inhibited gene expression of IL-6 and COX-2 and enhanced HO-1 synthesis in preadipocytes. Moreover, invasion assay showed the Hp suppressed migration of monocytes to preadipocytes. These findings suggest that the Hp may inhibit an inflammatory reaction in adipose tissue by regulating the expressions of pro-inflammatory and anti-inflammatory mediators, and by repressing monocytes/macrophages infiltration.
The Effects of Pro-inflammatory Cytokines by Cisplatin on the Death of Sensory Hair Cells.
Lee, Jeong-Han ; Park, Chan-Ny ; Park, Rae-Kil ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 542~549
DOI : 10.5352/JLS.2008.18.4.542
Cisplatin (cis-diamminedichloroplatinum II : CDDP) is the most widely used anticancer drug against a variety of human neoplasms. However, its clinical use is limited by the onset of severe side effects, including ototoxicity and nephrotoxicity. Even though a number of evidences in cytotoxic mechanism of cisplatin have been suggested, the role of pro-inflammatory cytokines in cisplatin cytotoxicity of auditory cells has not yet been demonstrated. Herein our data clearly demonstrated that cisplatin decreased the viability of HEI-OC1 auditory cells, which was inhibited by the addition of neutralizing
and anti-IL-6 antibodies. Consistently, Neutralization with antibodies against pro-inflammatory cytokines ameliorated the cell death and disarrangement of cochlea hair cell layers in the rat primary cochlear explants which were treated with cisplatin. Furthermore, exogeneous supplementation with free radical scavengers, including GSH and NAC, significantly prevented the cytotoxicity of cisplatin in the rat primary cochlea explants. We also observed that
was predominantly expressed in Deiters and Hensen's cells located in hair cell zone of cisplatin-treated cochlear explants. These findings suggest that pro-inflammatory cytokines, including
and IL-6, may play a pivotal role in the pathophysiology of hair cell damages caused by ototoxic drug cisplatin.
Monitoring on Alcohol Fermentation Properties of Red Ginseng Extracts.
Kim, Seong-Ho ; Kang, Bok-Hee ; Noh, Sang-Gyun ; Kim, Jong-Guk ; Lee, Sang-Han ; Lee, Jin-Man ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 550~555
DOI : 10.5352/JLS.2008.18.4.550
This study focused on alcohol fermentation properties of red ginseng extracts using Saccharomyces cerevisiae JF-Y3. Central composite design was employed to investigate the influence of red ginseng extract content (
, v/v) and yeast extract (
, w/v) on the properties of alcohol fermentation added with red ginseng extracts. Yeast cell growth was affected both by red ginseng extract content and yeast extract content, and red ginseng extract content had a greater effect on yeast cell number than yeast extract content. Yeast cell number increased along with decrease of the red ginseng extract content and with increase of yeast extract content. Alcohol content was maximal at 30% red ginseng extracts and 0.50% yeast extract and the predicted maximum value of alcohol content was 12.45%. Brix degree and total sugar content were significant within 1% level (p<0.01), and brix degree was affected both by red ginseng extract and yeast extract content. Total sugar content was significantly affected by red ginseng content.
Optimization of Fermentation Condition for Red Ginseng Wine Using Response Surface Methodology.
Kim, Seong-Ho ; Kang, Bok-Hee ; Noh, Sang-Gyun ; Kim, Jong-Guk ; Lee, Sang-Han ; Lee, Jin-Man ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 556~564
DOI : 10.5352/JLS.2008.18.4.556
Response surface methodology was used to monitor the optimization of fermentation conditions for red ginseng wine. A central composite design was applied to investigate the effects of independent variables, fermentation temperature (
), fermentation time (
) and initial pH (
) on dependent variables, physicochemical characteristics and effective ingredients. Alcohol and total sugar content were significantly affected both by fermentation temperature and time. Crude saponin content was greatly affected by fermentation time, and pH was significantly affected by initial pH. Fermentation time and initial pH had a greater effect on ginsenoside content than fermentation temperature. Ginsenoside content increased along with fermentation time and initial pH. We elicited a regression formula for each variable, and superimposed the total optimum points of fermentation conditions for physicochemical characteristics and the effective constituents. The predicted values at the optimum fermentation conditions were at
day in initial pH
Rough Computational Annotation and Hierarchical Conserved Area Viewing Tool for Genomes Using Multiple Relation Graph.
Lee, Do-Hoon ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 565~571
DOI : 10.5352/JLS.2008.18.4.565
Due to rapid development of bioinformatics technologies, various biological data have been produced in silico. So now days complicated and large scale biodata are used to accomplish requirement of researcher. Developing visualization and annotation tool using them is still hot issues although those have been studied for a decade. However, diversity and various requirements of users make us hard to develop general purpose tool. In this paper, I propose a novel system, Genome Viewer and Annotation tool (GenoVA), to annotate and visualize among genomes using known information and multiple relation graph. There are several multiple alignment tools but they lose conserved area for complexity of its constrains. The GenoVA extracts all associated information between all pair genomes by extending pairwise alignment. High frequency conserved area and high BLAST score make a block node of relation graph. To represent multiple relation graph, the system connects among associated block nodes. Also the system shows the known information, COG, gene and hierarchical path of block node. In this case, the system can annotates missed area and unknown gene by navigating the special block node's clustering. I experimented ten bacteria genomes for extracting the feature to visualize and annotate among them. GenoVA also supports simple and rough computational annotation of new genome.
Advances of Genome Research in Livestock Animals
Song, Ki-Duk ; Cho, Byung-Wook ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 572~579
DOI : 10.5352/JLS.2008.18.4.572
Genome research in economic animals has progressed rapidly in recent years, transforming from primitive genome maps to quantitative/qualitative trait maps that are indispensable to gene discovery. These advances have been benefited from the result of animal genome sequencing projects and functional genomics that are being extensively applied in livestock animal research following the development of large expressed sequences tags (ESTs). Genome sequencing efforts will provide information to QTL study by larger scale single nucleotide polymorphisms (SNPs) association study. Comparative genomics which is applying the information from human genome research as well as rodents model has contributed to important discoveries in economic animal genome research. These efforts will speed up much denser QTL maps development for phenotypic traits which are not easy to measure and to be identified by quantitative genetics  and lead to development of convincing markers associated with economically important trait, which will be eventually applied to livestock industry. In addition to practical application, animal genome research will enrich the understanding of human physiology in terms of genome biology.
Immunohistochemical Expression of O
-methylguanine-DNA Methyltransferase (MGMT) in Korean Patients with Non-Small Cell Lung Cancer.
Lee, Kyung-Eun ; Hong, Young-Seoub ; Choi, Phil-Jo ; Roh, Mee-Sook ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 580~584
DOI : 10.5352/JLS.2008.18.4.580
methyltransferase (MGMT) is a DNA repair protein that protects cells against the carcinogenic effects of alkylating agents. The loss of MGMT expression was commonly known due to hypermethylation of CpG islands in its promoter region. We evaluated the expression of MGMT by immunohistochemistry in order to examine the relationship between loss of MGMT expression and clinicopathological characteristics in 74 Korean patients with non-small cell lung cancers. Loss of MGMT was detected in 25 (33.8%) of 74 cases. The loss of MGMT expression was frequently seen in the adenocarcinoma than in the squamous cell carcinoma (p=0.021). However, there was no significant differences between loss of MGMT expression and other clinicopathological characteristics, including age, gender, smoking status, tumor size, tumor T stage, and lymph node metastasis (p>0.05). In conclusion, loss of MGMT expression was related with the histologic type of lung cancer. Further methylation study of MGMT promoter is needed to evaluate the relationships with immunohistochemical expression of MGMT and to clarify the role of MGMT in lung cancer.
Closed Conformation of a Human Phosphatase, Chronophin under the Reduced Condition.
Cho, Hyo-Je ; Kang, Beom-Sik ;
Journal of Life Science, volume 18, issue 4, 2008, Pages 585~589
DOI : 10.5352/JLS.2008.18.4.585
Chronophin is a phosphatase responsible for the dephosphorylation of cofilin, which regulates the rearrangement of actin cytoskeleton. It is also known as a phosphatase for pyrodoxal 5'-phosphate (PLP), an active form of vitamin
, and maintains the level of PLP in the cytoplasm. Since this phosphatase belongs to a HAD subfamily containing a cap domain, it is expected to undergo a conformational change for the binding of a substrate. However, the crystal structure of chronophin has a disulfide bridge between the cap and core domains preventing a movement of the cap domain against the core domain. It is possible that the disulfide bond between C91 and C221 was formed by an oxidation during the crystallization. Here, we obtained chronophin crystals under a reduced condition and determined the crystal structure. This reduced chronophin does not contain a disulfide bridge and shows a closed conformation like the oxidized form. It implies that an active chronophin binds its substrate under the closed conformation without the disulfide bond and shows a high substrate specificity in the cell.