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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
Selecting the target year
Effects of Apopotosis on Induction of Resveratrol in Human Lung Cancer A549 Cells
Chang, Jeong-Hyun ; Kwon, Heun-Young ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 591~597
DOI : 10.5352/JLS.2008.18.5.591
Resveratrol (3,5,4'-trihydroxystilbene, RSVL) is a supplement found mostly in grape skins. RSVL is known to exhibit potent antiproliferative activity against various cancer cell lines. The aim of this study was to examine the potential of RSVL to induce apoptosis in lung cancer A549 cells and to assess the mechanism of RSVL-induced apoptosis. Five different assays were employed in this study: cell viability and cytotoxicity, morphological assessments of apoptotic cells, quantitative DNA fragmentation analysis, and western blot analysis. The results indicated that RSVL has cytotoxic effects in A549 cells in a concentration- and time-dependent manner. And nuclear morphological changes of cells were observed by RSVL. RSVL regulates the cell cycle, especially
phase. Also, RSVL induces apoptosis of A549 cells via Caspase3, CAD and PARP-dependent fashion.
Effects of Farnesyltransferase Inhibitor Lonafarnib on Insulin Actions
Oh, Myung-Ju ; Yi, Sun-Ju ; Jeong, Young-Hwa ; Paik, Young-Ho ; Jhun, Byung-Hak ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 598~604
DOI : 10.5352/JLS.2008.18.5.598
Lonafarnib, a orally bioavailable nonpeptide tricyclic farnesyltransferase inhibitor, inhibits the prenylation of Ras and is currently under clinical trials in cancer patients. In the present studies, we examined the effects of lonafarnib on insulin actions including DNA synthesis, c-Jun expression, membrane ruffling and glucose uptake in insulin-responsive
cells and 3T3-L1 adipocytes. Using pan-Ras and isoform-specific anti-Ras antibodies, lonafarnib treatment specifically inhibited the prenylation of endogenous H-Ras in HIRc-B cells in dose-dependent manner, but not those of K- and N-Ras. Lonafarnib partially inhibited insulin-stimulated DNA synthesis in a dose-dependent manner, while it did not affect normal serum-mediated HIRc-B cell proliferation. Other insulin actions including c-jun expression and membrane ruffling in HIRc-B cells and glucose uptake in 3T3-L1 adipocytes were not affected. These results suggest that lonafarnib has a partial adverse effect on insulin actions.
The Calcineurin Inhibition by Overexpression of Cabin-1 Has a Potential Therapeutic Effect in Rheumatoid Arthritis
Kim, Hei-Jung ; Jin, Ing-Nyol ; Ryoo, Zae-Young ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 605~612
DOI : 10.5352/JLS.2008.18.5.605
Rheumatoid arthritis (RA) is characterized by a tumor-like expansion of the synovium, which is composed of proliferation, infiltrating leukocytes and angiogenesis. Calcineurin is expressed and plays a critical role in inflammatory arthritis. Calcineruin binding protein (Cabin)-l associates with calcineurin, leading to a non-competitive inhibitor of calcineurin activity. We investigated the expression pattern of calcineurin in inflammatory arthritis. The overexpression of Cabin-1 inhibited calcineurin phosphatase activity in in vitro. Moreover, we produced the T cell-specific Cabin-I transgenic mice but they died without transmitting the transgene to offspring. The Cabin-1 transgenic mice have a dominant lymph node expression and a wide tissue distribution. It is postulated that transgenic mice died from the critical effects of the transgene on T cell development or activation. Data suggests that the inhibition of calcineurin by the overexpression of Cabin-1 may be an effective strategy for the suppression of inflammatory arthritis.
Analysis of Thermoprotective Activity Induced by Heat Shock Protein 70 in Plants Using Two-Dimensional Difference Gel Electrophoresis
Cho, Eun-Kyung ; Choi, Young-Ju ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 613~619
DOI : 10.5352/JLS.2008.18.5.613
The effect of heat shock protein 70 (HSP70) on soluble proteins extracted from seedlings exposed to high temperature was examined by two-dimensional gel electrophoresis (2-DE). NtHSP70-1 (AY372069), which is an HSP70 that is induced by heat stress in Nicotiana tabacum, was studied to analyze the protective role of plant HSP70. First, transgenic tobacco plants that constitutively expressed elevated levels of the tobacco HSP70, NtHSP7D-1, and transgenic plants containing either the vector alone or the NtHSP70-1 gene in the antisense orientation were constructed. Plants with altered NtHSP70-1 levels were subjected to immunoblotting, and seedlings with enhanced levels of NtHSP70-1 in their transgenic sense lines were observed to show tolerance to heat stress. In the analysis using 2-DE, 28 putative proteins were differentially expressed in the comparison of three kinds of transgenic seedlings after high temperature/recovery treatment (
) with the seedlings under normal condition (
). In transgenic sense seedlings, the proteins were maintained or increased, whereas proteins in the transgenic seedlings, containing either the vector alone or the NtHSP70-1 in the antisense orientation, were present in decreased amounts or had disappeared. These results suggested that NtHSP70-1 as molecular chaperone protecting proteins from aggregation induced by heat stress may play an important role in acquisition of thermotolerance.
Culture Conditions for Glutathione Maximum Production by Saccharomyces cerevisiae FF-8 in Bioreactor
Cha, Jae-Young ; Park, Sang-Hyun ; Heo, Jin-Sun ; Park, Bo-Kyung ; Lee, Jin-Woo ; Kim, Jung-Wook ; Cho, Young-Su ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 620~624
DOI : 10.5352/JLS.2008.18.5.620
As glutathione is now widely used in medicine, functional health food and cosmetic industry, the commercial demand for glutathione has been expending. We have previously reported that Saccharomyces cerevisiae FF-8, an intracellular glutathione producing yeast strain, was isolated from Korean traditional rice wine. Glutathione production by FF-8 strain was 90 mg/l from basal YM medium in flask-scale fermentation, but that in optimal YM medium increased 2.27-fold to 204 mg/l. As large scale fermentation is generally applied to achieve more efficient production of bioactive components, this study was also tried to optimization of fermentation parameters to enhance the glutathione production using S. cerevisiae FF-8 in bioreactor process. The highest cell growth and glutathione concentrations of S. cerevisiae FF-8 in 7 l fermenter were observed to be the culture conditions containing culture temperature
, adjusted initial pH 6.0, agitation speed 200 rpm, and aeration rate 2.0 vvm. The maximum glutathione production by FF-8 was cultured under the inner pressure
in a 100 l bioreactor and it was 255.8 mg/l. The investigation of significant parameters for improving the glutathione production could be very useful in optimizing processes.
Cloning, Expression and Characterization of β-agarase Gene from a Marine Bacterium, Pseudoalteromonas sp. JT-6
Tao, Xueying ; Wu, Jing ; Kim, Kyoung-Sook ; Jing, Wu ; Lee, Young-Choon ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 625~630
DOI : 10.5352/JLS.2008.18.5.625
A gene (agaA6) encoding an extracellular agarase from a marine bacterium, Pseudoalteromonas sp. JT-6, was cloned, sequenced and expressed in Escherichia coli. It comprised an open reading frame of 1338 base pairs and encodes a protein of 445 amino acids with a predicted molecular weight of 50,150 daltons. The entire amino acid sequence of this agarase gene showed 99% identity with the agaA gene from Janthinobacterium sp. SY12. It consists of a signal peptide, a glycoside hydrolase family 16
domain and a carbohydrate-binding domain. The recombinant agarase was overexpressed and purified to homogeneity. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant enzyme were around
and 9.0. The enzyme was an endo-type
and hydrolyzed agarose to several oligosaccharides.
Effects of Exercise Pre-conditioning on Memory and Cell Proliferation in the Hippocampus of the Rats with Streptozotocin-induced Alzheimer's disease
Jeong, Il-Gyu ; Yoon, Jin-Hwan ; Lee, Hee-Hyuk ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 631~638
DOI : 10.5352/JLS.2008.18.5.631
Physical activity is known to slow the onset and progression of Alzheimer disease. In the present study, we investigated the effects of exercise pre-conditioning on hippocampal cell proliferation and memory in intracerebroventricular streptozotocin-induced Alzheimer's disease rats. Male (8 weeks old) SpragueDawley rats were used in the present study. Rats in the exercise pre-conditioning group were forced to run on a treadmill for 30 min daily for 14 days before lesioning. Rat model for Alzheimer's disease was made by injection of streptozotocin (STZ) (1.5 mg/kg) into a bilateral intracerebroventricular (ICV). Passive avoidance test was performed in order to evaluate the memory ability. After memory testing, the rat brains were removed, and 5-bromo-2'-deoxyuridine (BrdU) immunohistochemistry for the detection of cell proliferation were then performed. ICV STZ-injected rats showed a severe deficit in memory associated with decreased cell proliferation in the hippocampus. In contrast, exercise pre-conditioning demonstrated significantly enhanced hippocampal cell proliferation and improved memory performance in ICV STZ-injected rats. These results suggest that exercise pre-conditioning may enhance memory function by increasing cell proliferation in Alzheimer's type dementia.
Isolation of Bacteria Producing Cell Wall Degrading Enzyme and Characterization of CMCase.
Lee, Yong-Seok ; Park, In-Hye ; Zhou, Yi ; Kim, Keun-Ki ; Choi, Si-Lim ; Choi, Yong-Lark ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 639~645
DOI : 10.5352/JLS.2008.18.5.639
A CMCase-producing bacterium, Klebsiella sp. Sc, was isolated from Busan area's soil sample. This strain was identified on the basis of phylogenetic analysis of the 165 rDNA and rpoB (RNA polymerase B subunit) sequences. The gene encoding CMCase was cloned by genome library. The CMCase gene consisted of an open reading frame of 1,002 nucleotides and encodes 333 amino acids with a deduced molecular weight of 37,666 Da. The deduced amino acid sequence of the CMCase from Klebsiella sp. exhibits 95% identities and 97% positives to those from Cellulomonas uda and 58% identities and 72% positives to those from Erwinia chrysanthemi. The CMCase contain a conserved motif with the consensus sequence, ASDGDTLIAWALLRAQKQW. This result shows CMCase protein from Klebsiella sp. Sc belongs to glycoside hydrolase family 8, A-[ST]-D-[AG]-D-X(2)-[IM]-A-[SA]-[LIVM]-[LIVMG]-A-X(3)-[FW].
Biochemical Properties of EmeA, a Multidrug Efflux Pump, from Enterococcus faecalis.
Kwon, Hyun-Ju ; Kim, Byung-Woo ; Kim, Kwang-Hyeon ; Kim, Young-Hee ; Lee, Eun-Woo ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 646~652
DOI : 10.5352/JLS.2008.18.5.646
It was reported that expression of emeA gene, which was cloned from Enterococcus faecalis ATCC 29212, is responsible for the resistance against several antibiotics and for the efflux drugs, like as DAPI and Hoechst 33342. In this study, emeA gene was expressed in Escherichia coli KAM32, which is hypersensitive to many antibiotics and antimicrobial agents, and the biochemical properties of EmeA, a multidrug efflux pump, were investigated. EmeA showed 12 transmembrane domain structure which was constituted with 393 amino acids. EmeA showed significant homology in amino acid sequence with Bit of Lactococcus lactis, NorA of Staphylococcus aureus, and TetA of E. coli. EmeA expressing cells showed significantly lower accumulation level of ethidium bromide and acriflavine than EmeA non-expressing cells. Moreover, EmeA expressing cells represented efflux activity of ethidium bromide and acriflavine from the cells in an energy-dependent manner. This efflux activity was strongly inhibited by reserpine, which was known as a strong inhibitor of many drug efflux pumps.
Inhibitory Effect of Mulberry Extracts on Angiogenesis in Porcine Pulmonary Artery Endothelial Cells
Lee, Suk-Hee ; Kim, Hwan-Gyu ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 653~659
DOI : 10.5352/JLS.2008.18.5.653
Endothelial cells release proteinases to degrade the extracellular matrix for their migration and sprouting in vivo. In the present study, we investigated the inhibitory effect of mulberry extracts on the migration and sprouting activity of porcine pulmonary artery endothelial cells (PPAECs). Mulberry extracts significantly inhibited migration and sprouting of PPAECs. Mulberry extracts inhibited expressions and secretions of matrix metalloproteinase-2 (MMP-2) and MMP-9. Our results suggest that the inhibitory effects of mulberry extracts on migration and sprouting of PPAECs are, in part, associated with the decreased secretion of MMP-2 and MMP-9.
Influence of Ammonium against Cadmium Effect on in vitro Growth, Chlorophyll and RubiscojRubisco Activase in Tobacco Leaves.
Cho, Eun-Young ; Roh, Kwang-Soo ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 660~667
DOI : 10.5352/JLS.2008.18.5.660
Influence of ammonium against cadmium (Cd) effects on in vitro growth, chlorophyll and rubisco/rubisco activase was investigated in tobacco grown on M5 medium. In vitro growth reduced by Cd was recovered by
, and this recovery was most significant when tobacco was treated with 80 mM
. Chlorophyll levels were also recovered by 80 mM
. Rubisco content at 80 mM
was significantly increased than that at other treatments. Rubisco activity showed patterns of change similar to rubisco content. These data suggest that rubisco content was associated with activity of rubisco, and that the content and activity of rubisco reduced by Cd were recovered by ammonium. The degree of intensity of 55 and 15 kD polypeptides identified as the large and small subunit of rubisco by SDS-PACE analysis at 80 mM
was significantly higher than that at other treatments. Under the assumption that effects of ammonium on rubisco may be related to rubisco activase, its content and activity were determined. The rubisco activase content at 80 mM
was more increased than other treatments. A similar change pattern was also observed in activity of rubisco activase.
Antibacterial Activities of Suaeda maritima Hot Water Extracts
Moon, Youg-Gun ; Jang, Ik-Soo ; Heo, Moon-Soo ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 668~673
DOI : 10.5352/JLS.2008.18.5.668
To develop natural food preservatives, hot water extracts were prepared from the Suaeda maritima and antibacterial activities were examined against 12 microorganisms which were food borne pathogenic bacteria, food poisoning microorganisms and food-related bacteria. Hot water extracts exhibited antibacterial activities against 5 Gram positive and 7 Gram negative bacteria by agar diffusion method. The antibacterial activities and cell growth inhibition were investigated on each strain with the different concentrations of Suaeda maritima extracts. Antibacterial activities were detected in root, stem, fruit extracts of Suaeda maritima. However stem and fruit extracts showed weak antibacterial activity against the tested microorganisms. Root extracts showed the highest antibacterial activities against microorganisms tested, such as Bacillus cereus, Bacillus subtilis, Vibrio parahaemolyticus, and Staphylococcus aureus.
Kinetics Model Fitting of Photosynthesis and Growth in Chlorella sp.
Kang, Byeong-Chul ; Kang, Soon-Tae ; Wang, Hui-Chih ; Sur, Zee-Won ; Kim, Chang-Hoon ; Cho, Man-Gi ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 674~679
DOI : 10.5352/JLS.2008.18.5.674
We investigated which is the best kinetic model for expression of microalgal growth and photosynthesis by irradiance in green microalgae, marine Chlorella sp. is cultivated under 5 light intensities (27, 54, 81, 140, and 281
). The maximum of specific growth rate and photosynthesis at light intensity of 81
were observed as 0.35/day and 0.94
, respectively. To describe the effect of irradiance, we adapted both types for kinetic model fitting, saturation and inhibition models, Tamiya's modified Michaelis- Menten equation, hyperbolic tangent and Poisson function as saturation model, Steele's exponential and Andrew's function as the other and made the specific growth rate- irradiance response curve and photosynthesis- irradiance response curve. In case of microalgal growth, one of the saturation models, the hyperbolic tangent function was the most suitable, otherwise, the Steele's exponential function, one of the inhibition models, was suitable for photosynthesis.
Effect of Extracts from Dried Mackerel on Antioxidant Activity and Inhibition of Growth of Human Cancer Cell Lines
Jang, Joo-Ri ; Choi, Hyung-Ju ; Kim, Kyung-Kun ; Lim, Sun-Young ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 680~685
DOI : 10.5352/JLS.2008.18.5.680
This study was carried out to determine the inhibitory effects of solvent extracts from dried mackerel on
oxidative stress and growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells). In order to determine the protective effect on
oxidative stress, DCFH-DA (2',7'-dichlorodihydrofluorescin diacetate) assay was conducted. All fractions including crude extracts of dried mackerel appeared to significantly reduce the levels of intracellular reactive oxygen species (ROS) (p<0.05). Among the fractions, 85% methanol fraction showed a slightly higher protective effect on production of lipid peroxides. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05) and the inhibitory effect was more significant on the growth of HT1080 cells. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p<0.05). These results indicate that the consumption of mackerel may be recommended as a potent functional food for preventing cellular oxidation and cancer.
Conversion to Cardiopulmonary Bypass during Attempted Off-pump Coronary Bypass Grafting;An Analysis of Predictors and Clinical Outcomes.
Moon, Seong-Min ; Kim, Chong-Rak ; Kim, Yun-Tae ; Choi, Seok-Cheol ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 686~693
DOI : 10.5352/JLS.2008.18.5.686
We retrospectively sought to evaluate predictors and clinical outcomes of conversion to cardiopulmonary bypass during attempted off-pump coronary bypass grafting (OPCAP). Predictors and clinical outcomes in 24 patients underwent elective on-pump coronary bypass grafting (ONCAB) (an elective group) were compared with they in 22 patients required conversion from OPCAB to ONCAB (a conversion group). Causes of conversion from off-pump coronary bypass grafting (OPCAB) to ONCAB were hemodynamic instability (45.4%) and cardiac conduction instability (40.9%). The predictors for a conversion group were preoperative myocardial infarction (45.4%), left main coronary artery disease (36.3%) and low left ventricular ejection fraction (31.8%). Mean age, operation time, postoperative arrhythmia incidence and CRP level in a conversion group were higher than those in an elective group. OPCAP in ischemic heart disease requires careful attention, especially in patients with recognized potential preoperative risk factors. Particularly, gentle cardiac manipulation and meticulous myocardial stabilizer should be applied in left ventricular dysfunction patients.
A Taxonomic Study of the Genus Spiraea in Korea Using Sequences of ITS.
Huh, Man-Kyu ; Huh, Hong-Wook ; Lee, So-Young ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 694~700
DOI : 10.5352/JLS.2008.18.5.694
Genus Spiraea is a long lived woody species that is primarily distributed throughout Asia and Europe. We evaluated a representative sample of the fourteen taxa in Korea with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within genus. The molecular data allowed us to resolve well-Supported clades in taxa. Aligned nucleotide sequences of the length of ITS1 were nearly constant within genus Spiraea varying from 237 to 238. However, S. pubescens and S. salicifolia were showed 227 bp and 242 bp, respectively. Especially, the 5.8S subunit of all taxa of Spiraea was found to 157-165 bp nucleotides. However, aligned nucleotide sequences of the length of ITS2 vary from 263 to 271 bp. Total alignment length is 670 positions, of which 65 are parsimony-informative, 45 variable but parsimony-uninformative, and 410 constant characters. The phylogenic tree showed Korean taxa of genus Spiraea were well separated each other. S. prunifolia for. simpliciflora and S. blumei are conform one group and S. chartacea is the sister to them. S. fritschiana and S. S. miyabei are conform one group and S. japonica is the sister to them. S. pubescens was recognized as a distinct species.
Acanthopanax senticosus Extract Acts as an Important Regulator for Vascular Functions.
Choi, Seung-Min ; Park, Jong-Bong ; Kim, Jong-Min ; In, Kyung-Min ; Park, Heon-Yong ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 701~707
DOI : 10.5352/JLS.2008.18.5.701
Crude extracts of Acanthopanax senticosus (A. senticosus) have been used as galenicals. In this study we carried out a series of experiments to examine cardiovascular activities of crude extracts from A. senticosus. Whereas the extracts had no effect on the apoptosis of bovine aortic endothelial cells (BAECs), the extracts appeared to induce endothelial cell proliferation. In addition, endothelial cell migration was shown to be enhanced by the crude extracts, indicating that A. senticosus extracts likely act as an angiogenic factor. We also examined effects of A. senticosus extracts on nitric oxide (NO) production, leukocytic adhesion to endothelium, and homotypic aggregation of leukocytes. Both the NO production and the leukocytic adhesion to endothelial cells were revealed to be increased by treatment with A. senticosus extracts, but the homotypic aggregation of leukocytes was inhibited. Together, multiple roles of A. senticosus extracts in the cardiovascular system provide an insight on their broad applications as galenicals to treat cardiovascular diseases.
Encapsulation with Oyster Hydrolysate Using Alginate.
Cho, Kyung-Joo ; Choi, Yeung-Joon ; Ahn, Son-Cheol ; Baik, Moo-Yeol ; Kim, Byung-Yong ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 708~714
DOI : 10.5352/JLS.2008.18.5.708
Possibility of capsulation of oyster hydrolysate was investigated due to a low score of preference from the previous products, such as drinks and solid tablets of oyster hydrolysate. Encapsulation was made by emulsification to protect functional properties of oyster hydrolysate. Sodium alginate and calcium chloride were used as a wall material. Capsulation yield increased with increasing the capsule diameter and calcium chloride concentration, whereas capsulation yield decreased with increasing the oyster-hydrolysate concentration and stirring speed. Feasibility of capsulation of oyster hydrolysate was discussed for the processing of the functional food.
The Effects of the Complex Training Program on Cardiovascular Disease Risk Factors and Rate of Changes in the Elderly Women with Cardiovascular Disease.
Kwak, Yi-Sub ; Kim, Duk-Jung ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 715~723
DOI : 10.5352/JLS.2008.18.5.715
The purpose of this study was to investigate the effects of the regular resistance exercise with aerobic exercise training program participation on cardiovascular disease risk factors and rate of changes in the elderly women with cardiovascular disease. Subjects were 40 elderly women with cardiovascular disease who were
years old. We enrolled 20 exercise group into the participated 12 week complex exercise program. In measurement index, rest, exercise cardiovascular disease risk factor and rate of changes were measured by the submaximal exercise stress test. Results of this study were as follows; in the rest cardiovascular disease risk factor, % body fat, serum lipids and C-reactive protein (CRP) were increased in control group, but exercise group were decreased. In the exercise cardiovascular disease risk factor, control group were decreased, but exercise group were increased by the 12 week training program. In conclusion, we could know that not only the regular training might increase or change almost the cardiovascular function variables for serum lipid, body composition, exercise capacity between before and after complex training. Therefore, we might tell that complex training program could provide an excellent parameters for enforcing or keeping the cardiovascular function of elderly women with cardiovascular disease.
The Effects of Aquatic Rehabilitation Exercise Program on Serum Lipids and Atherogenic Indices in the Disabled People with Spinal Cord Injury
Kim, Duk-Jung ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 724~730
The purpose of this study was to investigate the effects of the aquatic rehabilitation exercise program on serum lipids and atherogenic indices in the disabled people with spinal cord injury men. The relation of aquatic rehabilitation exercise and increase physical function was determined using an 12 week aquatic rehabilitation exercise intervention study involving 30 untrained subjects with disabled people with spinal cord injury men. Subjects were divided into two groups: control group (n=15), experiment group (n=15). Results of this study were as follows; In the serum lipids, experiment group were significantly decreased by the 12 week aquatic rehabilitation program in the total cholesterol, TG, and increased HDL-C. In the atherogenic indices, experiment group were significantly decreased by the 12 week aquatic rehabilitation program in the TC/HDL-C and TG/HDL-C. Therefore, this study supports the conclusion that continuous aquatic rehabilitation exercise participation is the one of most effective methods to maintain daily manual rehabilitation training propulsion avoiding the risk of lipids metabolism.
Gene Targeted Mice to Study T Cell Development
Song, Ki-Duk ; Kim, Sang-Wook ; Lee, Mi-Rang ; Kim, Seon-Ku ; Shin, Taek-Soon ; Gang, Han-Seok ; Lee, Hong-Gu ; Jeon, Hae-Yeal ; Cho, Byung-Wook ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 731~737
DOI : 10.5352/JLS.2008.18.5.731
T cells develop in thymus, where immature progenitors undergo multi-stage developmental processes until mature CD4+ and CD8+ single positive T cells are generated and egress out to the periphery. Signals from T cell receptor are pivotal for T cell development in thymus as well as in periphery. Gene targeted mutant mice have contributed the understanding underlying mechanisms by dissecting the signaling pathways which governing these complex processes. Development of refined mutational system will be imperative to analyze the signaling pathways by taking consideration of context of 'timing and/or space' of gene function.
Anti-HIV RNAs and Viral Vectors for Gene Therapy
Park, Jeong-Hee ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 738~746
DOI : 10.5352/JLS.2008.18.5.738
Human immunodeficiency virus (HIV) therapy has been in continuous development since the discovery of HIV. Various RNA genes to inhibit HIV replication have been deployed such as RNA decoy, antisense RNA, ribozyme, as well as conventional drugs that inhibit viral proteins. Small interfering RNAs (siRNAs) against HIV have been intensively studied as tools for HIV gene therapy in recent years and become powerful tools to block the replication of HIV. The RNA molecules against HIV is easy to manipulate and highly efficient inhibitors of HIV however the levels of expression and the delivery system of theses RNA genes against HIV should be carefully examined to introduce the therapeutic RNAs into target cells without inducing any toxicity. Due to frequent emergence of escape mutants, multiple approaches to block HIV replication should be considered and the development of different type of anti-HIV RNA technologies could contribute to an establishment of highly efficient combinational RNA therapy against HIV. This review will focus on the recent developments in potential target sites for therapeutic RNAs to block HIV and on the consideration of different types of anti-HIV RNAs and their expression units, as well as viral vectors to deliver these RNA genes to host cells.
Anti-hypercholesterolemic Effects of Wasabia japonica in High Cholesterol-Fed SD Strain Rats
Lee, Young-Sun ;
Journal of Life Science, volume 18, issue 5, 2008, Pages 747~751
DOI : 10.5352/JLS.2008.18.5.747
This study was carried out to investigate the anti-hypercholesterolemic effects of Wasabia japonica (WJ) in hypercholesterolemic rats. Sprague-Dawley male rats were randomly assigned to one normal diet and three high cholesterol diet groups which contained 1% (w/w) cholesterol. After 4 weeks cholesterol diet, the groups of high cholesterol diet were classified to control (normal diet only), WJR5 (normal diet plus 5% WJ root) and WJL5 (normal diet plus 5% WJ leaf). There were no difference in the body weight gain, food intake and Food efficacy ratio (FER). The liver and kidney weights change of high cholesterol diet groups were higher than the normal diet group (p<0.05), but there was no significant difference of change between control and WJ diet groups. Concentration of serum HDL-cholesterol were significantly increased in WJ diet groups compared with control group (p<0.05). Concentration of serum LDL-cholesterol were significantly decreased in the groups of WJR5 diet compared with control group (p<0.05). The results of the study indicate that the diet of WJ can ameliorate the status of HDL-cholesterol and LDL-cholesterol in high cholesterol diet rats. In view of these results, WJ is expected to be an effective material for the anti-hypercholesterolemic in high cholesterol diet rats.