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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
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Genetic and Phylogenetic Relationships of Genus Hemerocallis in Korea Using ISSR
Choi, Joo-Soo ; Huh, Hong-Wook ; Lee, Seol-A ; Huh, Man-Kyu ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 753~758
DOI : 10.5352/JLS.2008.18.6.753
Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm
Phylogenesis of Halophila ovalis (R. Br.) Hook. fil. (Hydrocharitaceae) from An Island, Korea
Kim, Jeong-Bae ; Cho, Eun-Seob ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 759~763
DOI : 10.5352/JLS.2008.18.6.759
Halophila ovalis (R. Br.) Hook. fil. was first collected from the Ando, Yeosu, Korea in 2007. H. ovalis is Widely distributed from sub-tropical to even tropical areas and produces the seeds using bisexual reproduction. Its leaf shape was oblong to ovate. Its leaf blades were rigid in texture, with a strong support to the leaf. Erect shoots arose at irregular intervals along the rhizome. The distance between the intramarginal vein and leaves margin was small. Nucleotides in ITS 1 and ITS 2 regions between the Korean and Japanese H. ovalis were found to be 100% similar, whereas Korean H. ovalis was found to have four nucleotides in the positions of 202 bp to 206 bp for 5.8S. In the analysis of the phylogenetic relationship using NJ method, Korean H. ovalis had a monophyletic genetic tree with Japanese H. ovalis, but no phylogenetic relationship with types from the Philippines, Australia, Malaysia, and Vietnam. The first occurrence of H. ovalisin Korea was associated with a strong migration of gene flow from Japan and high water temperature caused by the variations in climate.
Analysis of CMTX Mutants Using Connexin Membrane Channels
Cheon, Mi-Saek ; Oh, Seung-Hoon ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 764~769
DOI : 10.5352/JLS.2008.18.6.764
Mutations in the human connexin 32 (Cx32) gene are responsible for X-linked Charcot-Marie-Tooth (CMTX) disease. Although over 300 different mutations have been identified the detailed molecular etiology of CMTX disease is poorly understood. Several studies reported that connexin membrane channels share most biophysical properties with their parental gap junction channels. In this study, two connexin mutant membrane channels (one mutant channel called the M34T channel in which the methionine residue at the
position of the Cx32 protein is replaced with threonine residue and the other mutant channel called the T86C channel in which the threonine residue at the
position is replaced with cysteine residue) associated with CMTX mutations were characterized at the single-channel level instead of using mutant gap junction channels. The biophysical properties of the M34T channel were very similar to those of the gap junction channel formed by M34T mutation. In addition, the mutant membrane channel study revealed the reversal of the gating polarity, the loss of fast gating and the gain of slow gating. The T86C channel also behaves like its parental wild type Cx32 membrane channel. Taken together, these results suggest that a study using connexin membrane channels is useful to characterize CMTX mutants.
Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Genes for Economic Traits in the Commercial Pig Breed
Kim, Sang-Wook ; Lee, Mi-Rang ; Kang, Han-Seok ; Kim, Seon-Ku ; Shin, Teak-Soon ; Lee, Hong-Gu ; Jeon, Hae-Yeal ; Kim, Kwan-Suk ; Do, Chang-Hee ; Choi, Bong-Hwan ; Kim, Tae-Hun ; Cho, Byung-Wook ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 770~775
DOI : 10.5352/JLS.2008.18.6.770
Several studies reported quantitative trait loci (QTL) for meat quality on porcine chromosome 2. For application of the chromosomal information to pig industry through using DNA technology, single nucleotide polymorphism (SNP) markers are developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 13 candidate genes. A total of 34 SNPs were identified in 11 PCR products, an average of one SNP in every 296 bp.PCR restriction fragment length polymorphism (RFLP) assays were developed for 11 SNPs and used to genotype four commercial pig populations in Korea. The SNP markers were used to map candidate genes in QTL and to clarify the relevance of SNP and quantitative traits.
Antibacterial Activities of Suaeda maritima Extract.
Moon, Young-Gun ; Song, Chang-Young ; Yeo, In-Kyu ; Kim, Gi-Young ; Heo, Moon-Soo ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 776~781
DOI : 10.5352/JLS.2008.18.6.776
To develop natural food preservatives, methanol and water extracts were prepared from the Suaeda maritima and their antibacterial activities were examined against 12 microorganisms which were food borne pathogens bacteria, food poisoning microorganisms and food-related bacteria. Methanol extracts exhibited antibacterial activities for the 5 Gram positve and 7 Gram negative bacteria by agar diffusion method, The antibacterial activities and cell growth inhibition were investigated on each strain with the different concentrations of Suaeda maritima extracts. Antibacterial activities were shown in root, stem, furit extracts of Suaeda maritima. However stem and fruit extracts showed weak antibacterial activity against the tested microorganisms. Root extracts showed the highest antibacterial activities against microorganisms tested, such as Bacillus cereus,Bacillus subtilis, Vibrio parahaemolyticus, Staphylococcus aureus. The highest antibacterial activity against bacteria test was found in the methanol extract.
Characterization and Distribution of Glycoconjugates in the Intestines of Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis.
Jeong, Gil-Nam ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 782~788
DOI : 10.5352/JLS.2008.18.6.782
The aim of this work is to show differences in the pattern of glycoconjugate composition in the intestines of four teleostean species (Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis). We compared four regions of all species studied. The specimens were processed and stained with nine kinds of biotinylated lectins (DBA, SBA, PNA, BSL- I , RCA- I , sWGA, UEA- I , LCA and Con A). Except for Sebastes schlegeli, no differences between regions were observed. The intestinal epithelium of Halichoeres poecilopterus possessed D-glucose/mannose residues in all regions.
-N-acetyl-D-galactosamine was distinctive along the intestines, although the pattern of diversity was different in Sebastes schlegeli, Bryzoichthys lysimus, and Takifugu pardalis. Additionally, the occurrence of Galactose-
-D-galactose were confirmed in the proximal, middle, and distal intestine of Sebastes schlegeli, while rectal intestine lacked these sugar residues. Along with
-N-acetyl-D-glucosamine and D-glucose/mannose were also determined in Bryzoichthys lysimus. Galactose-
-1,3-N-acetyl-D-galactosamine, D-galactose, and D-glucose/mannose were also present in Takifugu pardalis.
Purification of Vibrio anguillarum Growth Inhibition Factor Produced by Bacillus amyloliquefaciens H41.
Shin, Hyun-Chul ; Chung, Kyung-Tae ; Kim, Kwang-Hyun ; Kim, Byung-Woo ; Kwon, Hyun-Ju ; Lee, Eun-Woo ; Yum, Jong-Hwa ; Rhu, Eun-Ju ; Jeong, Yu-Jeong ; Kim, Young-Hee ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 789~795
DOI : 10.5352/JLS.2008.18.6.789
To study the possible use of probiotics in fish farming, we evaluated antagonism of antibacterial strain Bacillus amyloliquefaciens H41 against the fish pathogenic bacterium Vibrio anguillarum NCMB1. The purification of growth inhibition factor produced by B. amyloliquefaciens H41 was achieved by obtaining supernatant of this bacterium. The growth inhibition factor was purified to homogeneity by 70% ammonium sulfate precipitation, DEAE-sephadex A-50 ion exchange chromatography, sephadex G-200 gel filtration column chromatography, and sephadex G-50 gel filtration column chromatography with 40.8 fold of purification and 2.9% yield. The molecular weight of the purified growth inhibition factor was 48 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH and temperature for the growth inhibition factor were pH 7.5 and
, respectively. The activity of growth inhibition factor was enhanced slightly by some metal ions, such as
, but was inhibited by the addition of
. NaCl stability of the growth inhibition factor was observed with 50% residual activity at 3% NaCl concentration. Toxicity test showed that the purified B. amyloliquefaciens H41 growth inhibition factor did not affect the live of Japanese flounder (Paralichthys olivaceus) and the effectiveness was 78% of residual lethality compared to commercial antibacterial agents.
The Effect of Exercise Training on Aβ-42, BDNF, GLUT-1 and HSP-70 Proteins in a NSE/ APPsw-transgenic Model for Alzheimer`s Disease.
Eum, Hyun-Sub ; Kang, Eun-Bum ; Lim, Yea-Hyun ; Lee, Jong-Rok ; Cho, In-Ho ; Kim, Young-Soo ; Chae, Kab-Ryoung ; Hwang, Dae-Yean ; Kwak, Yi-Sub ; Oh, Yoo-Sung ; Cho, Joon-Yong ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 796~803
DOI : 10.5352/JLS.2008.18.6.796
Mutations in the APP gene lead to enhanced cleavage by
, and increased
formation, which are closely associated with Alzheimer`s disease (AD)-like neuropathological changes. Recent studies have shown that exercise training can ameliorate pathogenic phenotypes (
, BDNF, GLUT-1 and HSP70) in experimental models of Alzheimer`s disease. Here, we have used NSE/APPsw transgenic mice to investigate directly whether exercise training ameliorates pathogenic phenotypes within Alzheimer`s brains. Sixteen weeks of exercise training resulted in a reduction of
peptides and also facilitated improvement of cognitive function. Furthermore, GLUT -1 and BDNF proteins produced by exercise training may protect brain neurons by inducing the concomitant expression of genes that encode proteins (HSP-70) which suppress stress induced neuron cell damages from APPsw transgenic mice. Thus, the improved cognitive function by exercise training may be mechanistically linked to a reduction of
peptides, possibly via activation of BDNF, GLUT-1, and HSP-70 proteins. On the basis of the evidences presented in this study, exercise training may represent a practical therapeutic management strategy for human subjects suffering from Alzheimer`s disease.
Induction of Apaopotis by Water Extract of Cordyceps militaris (WECM) in Human Hepatocellular Carcinoma HepG2 Cells.
Kim, Kyung-Mi ; Park, Cheol ; Choi, Yung-Hyun ; Lee, Won-Ho ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 804~813
DOI : 10.5352/JLS.2008.18.6.804
Cordyceps militaris, the Chinese medicinal fungal genus Cordyceps, is reported to possess many pharmacological activities including immunological stimulating, anti-cancer, anti-virus and anti-infection activities. However, the molecular mechanisms of C. militaris on biochemical actions in cancer have not been clearly elucidated yet. In the present study, we investigated the anti-proliferative activity of the water extract of C. militaris (WECM) in human hepatocellular carcinoma HepG2 cells. It was found that WECM could inhibit the cell growth in a dose-dependent manner, which was associated with morphological changes and apoptotic cell death such as formation of apoptotic bodies and increased populations of apoptotic sub-G1 phase. Apoptotic cell death of HepG2 cells by WECM was connected with a up-regulation of pro-apoptotic Bax expression, tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 (WAF1/CIP1). In addition, WECM treatment induced the proteolytic activation of caspase-3 and a concomitant degradation and/or inhibition of poly (ADP-ribose) polymerase (PARP),
protein. Furthermore, caspase-3 inhibitor, z-DEVD-fmk, significantly inhibited WECM-induced apoptosis demonstrating the important role of caspase-3 in the observed cytotoxic effect. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of C. militaris.
Proteome Analysis of Responses to Ascochlorin in LPS-induced Mouse Macrophage RAW264.7 Cells by 2-D Gel Electrophoresis and MALDI-TOF MS.
Chang, Young-Chae ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 814~825
DOI : 10.5352/JLS.2008.18.6.814
Ascochlorin (ASC) is prenyl-phenol compound that was isolated from the fungus Ascochyta viciae. ASC reduces serum cholesterol and triglyceride levels, and suppresses hypertension, tumor development, ameliorates type I and II diabetes. Here, to better understand the mechanisms by which ASC regulates physiological or pathological events and induces responses in the pharmacological treatment of inflammation, we performed differential analysis of the proteome of the mouse macrophage RAW264.7 cells in response to ASC. In this study, we used a proteomic analysis of LPS-induced RAW264.7 cells treated by ASC, to identify proteins potentially involved in inflammatory processes. The RAW264.7 cell proteomes with and without treatment with ASC were compared using two-dimensional electrophoresis (2-D SDS-PAGE), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) and bioinformatics. The largest differences in expression were observed for the calreticulin (4-fold decrease),
(4-fold decrease) and vimentin (1.5-fold decrease). In addition, rabaptin was increased 3-fold in RAW264.7 cells treated with ASC. The expression of some selected proteins was confirmed by RT-PCR analysis.
The Effect of Octacosanol Supplement and Taekwondo Program on Blood Fatigue Substance, Immunoglobulin for Short Term Weight Loss in High School Male Taekwondo Players.
Ye, Jeong-Bok ; Lee, Sang-Ho ; Baek, Yeong-Ho ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 826~831
DOI : 10.5352/JLS.2008.18.6.826
The purpose of this study was to examine the effects of octacosanol and Taekwondo program on the fatigue substance, immunoglobulin after short term weight loss 21 male Taekwondo players. They were stay in a camp training together 6 days from same exercise and Octacosanol group and weight loss group is reduced over 5% weight loss by diet calorie restriction decrease gradually. Octacosanol intaked 2 times 20 mg after a meal. Control and weight loss group intaked placebo drug. The conclusions obtained from the above were as follow: Blood Fatigue Substance As lactate, The intra-group comparison decreased octacosanol group after loss weight. The between groups comparison, octacosanol group was superior to control and weight loss group. As lactate dehydrogenase, control and weight loss group increased after weight loss. As ammonia, The intra- group comparison decreased octacosanol group after loss weight. The between groups comparison, octacosanol group was superior to control and weight loss group. Immunoglobulin As IgA, IgG The intra-group comparison increased all group. As IgM, The were no significant differences between group. In conclusion, these results indicate that intake octacosanol after loss weight positive effects on the fatigue substance, Immunoglobulin for male Taekwondo players. Mter this study, We need to study about multilateral research for weight players.
Recovery and Utilization of Proteins and Lipids from the Washing Wastewater in Marine Manufacture by Isoelectric Point Shifting Precipitation Method;4. Utilization of the Recovered Protein Fractions as the Alternative Feed of Fish Meal.
Kim, Gwang-Woo ; Kim, Ga-Hyeon ; Ueo, Myung-Hee ; Kim, Ok-Seon ; Cho, Soon-Yeong ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 832~838
DOI : 10.5352/JLS.2008.18.6.832
Mackerel water-soluble protein fraction produced by washing the mackerel meat were concentrated by isoelectric point shifting precipitation process, and the concentrates were utilized as the alternative feed of fish meal. In the 1st aquaculture diet experiment for Israel common carp, the feed conversion ratio decreased in proportion to the rise in the percentage of the recovered protein containing a residual lipid, which was added to the fish meal. It was supposed that the low feed efficiency was because of lipid oxidation in the recovered protein fraction. In addition, 2nd aquaculture diet experiment for Israel common carp was conducted after removing the oxidized lipid in the recovered protein fish meal. When a portion of the fish meal was substituted by the recovered protein devoid of the residual lipid, the feed conversion ratio increased in proportion to the amount of the substitute being added to the fish meal. Therefore, the recovered protein fraction of the mackerel washing wastewater from mackerel processing factory could be used as the alternative feed of fish meal.
Optimal Culture Conditions on the Keratinase Production by Bacillus sp. SH-517.
Bang, Byung-Ho ; Rhee, Moon-Soo ; Lim, Ki-Hwan ; Yi, Dong-Heui ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 839~844
DOI : 10.5352/JLS.2008.18.6.839
A strain SH-517 which produce extracellular keratinase, was isolated from the soil of a poultry waste and a poultry factory. An isolate SH-517 was identified as Bacillus sp. based on its morphological and biochemical characteristics. The optimal culture conditions for the production of keratinase by Bacillus sp. SH-517 were investigated. The optimal medium composition for keratinase production was determined to be 2.0% chicken feather as carbon source, 0.5% beef extract as organic nitrogen source, 0.5%
as inorganic nitrogen source and 0.06% KCl, 0.05% NaCl, 0.04%
as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH of medium were shown
and 8.5 with shaking culture (180 rpm/min), respectively. The maximum keratinase production reached maximum of 125 units/ml/min after 42 hr of cultivation under the optimal culturing conditions.
Purification and Characterization of β-Lactamase Secreted from Bacillus sp. J105 Strain having β-Lectam Antibiotics Resistance.
Cho, Kyeong-Soon ; Kang, Byoung-Won ; Seo, Min-Jeong ; Lee, Young-Choon ; Lee, Jai-Heon ; Joo, Woo-Hong ; Choi, Yung-Hyun ; Lim, Hak-Seob ; Kim, Jeong-In ; Seo, Kwon-Il ; Jeong, Yong-Kee ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 845~851
DOI : 10.5352/JLS.2008.18.6.845
, secreted from Bacillus sp. J105 strain was purified to a single band on SDS-PAGE by ammonium sulfate precipitation, ion exchange column chromatography and gel-filtration. The molecular weight of the purified enzyme was 31 kDa on SDS-PAGE and its isoelectric point was 7.35. Optimal pH and temperature for enzymatic reaction were 5 and
, respectively. As a result of total amino acid composition analysis of the purified enzyme, Gly and Ala were occupied 14.1 and 13.3 mole %, respectively. Km and Vmax value of purified enzyme were 1.33 mM and 0.36 mM/ml using ampicillin as a substrate, respectively.
Effect of Bulnesia sarmienti Water Extract on Lipid Metabolism in Type-1 Diabetic Rats.
Park, Chang-Ho ; Kim, Jung-Ok ; Lee, Gee-Dong ; Kim, Kil-Soo ; Hong, Joo-Heon ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 852~857
DOI : 10.5352/JLS.2008.18.6.852
The effects of Bulnesia sarmienti water extract on lipid metabolism were investigated in type-1 diabetes rats. The rats were fed a basal diet and B. sarmienti water extract for 8 weeks. The rats were randomly assigned to each treatment group: normal, control, three kinds of treatment group (BWO: 0.45 mg/ml, BW1: 0.9 mg/ml, BW2: 1.8 mg/ml). After 8 weeks of experimental diets consumption, the body weights and food efficiency ratio of B. sarmienti water extract fed groups were not change with those in the control group. The concentration in plasma total-cholesterol, LDL-cholesterol, triglyceride were significantly decrease in the B. sarmienti water extract groups compared with those in the control group. Although it was not possible to observe blood glucose control effects of eight weeks of B. sarmienti water extract feeds in type-1 diabetes model, it was discovered that the feeds are highly effective in lowering the levels of blood lipid substances triglycerides, total-cholesterol, and LDL-cholesterol.
Biological Control of Plant Pathogens by Bacillus sp. AB02.
Kim, Keun-Ki ; Kim, Yong-Chul ; Choi, Young-Whan ; Sin, Taek-Sun ; Park, Ki-Do ; Kang, Ui-Gum ; Choi, Yong-Lark ; Park, Hyean-Cheal ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 858~864
DOI : 10.5352/JLS.2008.18.6.858
In the greenhouse fields for fruits and vegetables during the winter in Korea, there are serious damages by the sclerotium diseases due to the low temperature and humidity. This study was carried out to select an antagonic agent for the biological control of the sclerotium diseases. The 55 antagonic agents were selected from the rhizosphere in soil where the fruits and vegetables were cultivated in the green house fields, and strain AB02 among the tested isolates was estimated to be the strongest antagonist against the sclerotium disease. Using strain AB02, the antifungal spectrum was tested against 5 different plant pathogens. According to the results of the test, strain AB02 . showed the high antagonistic effect against Botrytis cinerea and Sclerotinia sclerotiorum. For the experiment of biological control against the sclerotium disease, it was estimated the suppression effect and the control effect by the strain in the pot experiment using the green perilla. According to the result of the pot experiments, the suppression effect was 40% and the control effect was 62%, respectively. For the stimulation effect of the tested plant growth by strain AB02 compared to the control, it was improved as 120% for the total length, 141% for the liveweight, 121% for the total number of leaves, 185% for the leaf area, and 327% for the liveweight of the root, respectively. Strain AB02 showing the antagonistic effect against the sclerotium disease and the stimulation effect for the plant growth was identified as Bacillus sp.
Effects of Regular Exercise and Diet on RMR and Hormonal Changes in Obese Women.
Park, Hyong-Ran ; Paik, Il-Young ; Jin, Hwa-Eun ; Kim, Young-Il ; Kwak, Yi-Sub ; Woo, Jin-Hee ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 865~870
DOI : 10.5352/JLS.2008.18.6.865
The purpose of this study was to examine resting metabolic rate (RMR) and thyroid hormonal (TSH,
) changes following weight loss by diet with regular exercise in obese women. The subjects of the present study were 7 women who were above 30% body fat. The subjects arrived into the laboratory in the morning after 12 hour overnight fasting. All subjects measured RMR, % body fat, and fat free mass at weight loss program start time, after 4 weeks and after 8 weeks. All the RMR values were expressed as absolute value (kcal/day), absolute value/FFM (kcal/day/FFM), and absolute value/BSA (kcal/
/hr), and were calculated predicted RMR value minus actual RMR value. Also, correlation of blood thyroid hormonal (TSH,
) secretion and RMR were analyzed. There were significant differences in weight, % body fat, and BSA following diet with exercise (p<0.05). Also, there was a difference between predicted RMR and actual RMR value following weight loss (p<0.05). We also examined the hormonal changes according to weight loss. After weight loss, the level of TSH and
were higher than before. But there were no significant differences. Also, the level of
was lower than rather before but there was no significant difference. Among the anthropometric factors, FFM was highly correlated (r
Analysis of Optimum Condition for Alcoholic Drink Production Using Onion Extract.
Kim, Sam-Woong ; Oh, Eun-Hye ; Jun, Hong-Ki ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 871~877
DOI : 10.5352/JLS.2008.18.6.871
Onions are considered to be a promising source of the alcoholic drink because they are rich in sugar, amino acids and various nutrients. To isolate strains of Saccharomyces cerevisiae producing ethanol of higher concentration, 19 strains were subjected to screening. Among them, the strain producing the highest concentration of ethanol was OJ-8 strain. Onion`s oder was effectively removed by treatment for 30 min with 10% (w/v) charcoal against medium and then heat treatment of onion extract for 40 min at
. The optimum conditions for alcoholic fermentation was investigated in medium containing the onion extract. The optimal conditions for ethanol production was obtained by standing culture for 5 days at
with 5% inoculum volume.
Improvement of the Phosphate Solubilization Microorganism by the Introduction of Glucose Dehydrogenase Gene into Aeromonas hydrophila DA33.
Park, In-Hye ; Song, Ok-Ryul ; Lee, Yong-Seok ; Kang, Ui-Gum ; Choi, Si-Lim ; Choi, Yong-Lark ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 878~883
DOI : 10.5352/JLS.2008.18.6.878
Aeromonas hydrophila DA33 was isolated from cultivated soils as a bacteria having high abilities to solubilize inorganic phosphate. Glucose dehydrogenase gene (gdh) was cloned from Escherichia coli. The recombinant plasmid, pGHS containing glucose dehydrogenase gene was introduced into A. hydrophila DA33 in order to improve the activity of phosphate-solubilizing. The transformant harboring the gdh gene, A. hydrophila pGHS/DA33 increased enzyme activity. The strain also increased the gluconic acid generation that was effective for phosphate solubilization. It was possible that the strain containing pGHS produced higher solubilized phosphate with tri-calcium phosphate as the unique (P) source, in comparison with that of wild type without plasmid. These results suggest that the strain, A. hydrophila pGHS/DA33 is expected as effective biofertilizer for phosphate solubilization.
Genistein-induced Growth Inhibition was Associated with Inhibition of Cyclooxygenase-2 and Telomerase Activity in Human Cancer Cells.
Kim, Jung-Im ; Kim, Seong-Yun ; Seo, Min-Jeong ; Lim, Hak-Seob ; Lee, Young-Choon ; Joo, Woo-Hong ; Choi, Byung-Tae ; Jeong, Yong-Kee ; Choi, Yung-Hyun ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 884~890
DOI : 10.5352/JLS.2008.18.6.884
Genistein, an isoflavone in soybean products, is a potential chemopreventive agent against various types of cancer. There are several studies documenting molecular alterations leading to cell cycle arrest at G2/M phase and induction of apoptosis; however, its mechanism of action and its molecular targets on the prostaglandin
) production and telomere length regulation in human cancer remain unclear. In this study, we investigated the effect of genistein on the levels of cyclooxygenases (COXs) and telomere regulatory components of several human cancer cell lines (T24, human bladder carcinoma cells; U937, human leukemic cells; AGS, human stomach adenocarcinoma cells and SK-MEL-2, human skin melanoma cells). Genistein treatment resulted in the inhibition of cancer cell proliferation in a concentration-dependent manner. It was found that genistein treatment markedly decreased the levels of COX-2 mRNA and protein expression without significant changes in the expression of COX-1, which was correlated with a decrease in
synthesis. Genistein treatment also partly inhibited the levels of human telomerase reverse transcriptase (hTERT) as well as human telomerase RNA (hTR) and telomerase-associated protein (TEP)-1, and the activity of telomerase. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of genistein.
The Role of Mast Cells in Innate and Adaptive Immunity.
Kim, Young-Hee ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 891~896
DOI : 10.5352/JLS.2008.18.6.891
The function of mast cells as effector cells in allergy has been extensively studied. Mast cells activated through high affinity IgE-receptor (
) release diverse mediators, and lead to smooth muscle constriction, vasodilation, increase of vascular permeability, leukocyte recruitment and activation, mucus secretion, and tissue proliferation and remodeling. However, various other immunological and non-immunological signals can lead to the activation of mast cells. In resent years, mast cells have been identified to be involved in a complex range of immune functions. Mast cells can be important as key players in the regulation of innate as well as adapted immune responses, and may influence the development of allergy, autoimmune disorder and peripheral tolerance. This review summarizes the recent advances in the understanding of effector functions of mast cells in immune responses.
Deep Seawater Increases Dendritic Branches of Cultured Rat Hippocampal Neurons
Lee, Hyun-Sook ; Nam, Kyung-Soo ; Shon, Yun-Hee ; Moon, Il-Soo ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 897~901
DOI : 10.5352/JLS.2008.18.6.897
Deep seawater (DSW; deep ocean water) is pure, rich in inorganic materials which have attracted attention for various applications. In this study we investigated the effects of the DSW upwelled from the East Sea, offshore Yang Yang (Korea) on the morphological differentiation of cultured rat hippocampal neurons, which were grown in the minimal essential medium containing 10% (v/v) fetal bovine serum and 25% (v/v) DSW with various hardness. DSW had no effect on initial morphological differentiation (17 hr post-plating). When observed on DIV3, 7, 14, and 17, low hardness (0 and 200) DSW reduced dendritic branching. However, dendritic branches within
diameter from the center of soma nearly doubled in neurons grown in hardness 1,000 DSW-containing media. DSW with hardness 600 was more or less same as control groups. These results indicate that DSW with appropriate hardness ameliorates neuronal health.
Analysis of Microsatellite DNA Polymorphisms for Pedigree Verification in Kyungju Dog(Dongkyung-i).
Lee, Eun-Woo ; Choi, Seog-Gyu ; Cho, Gil-Jae ;
Journal of Life Science, volume 18, issue 6, 2008, Pages 902~906
DOI : 10.5352/JLS.2008.18.6.902
In this study, we analyzed the microsatellite DNA poly-morphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 microsatellite loci were
(mean 0.7587) and
(mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (>0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parentage testing were noted significant differences compared with breeders. These results show basic information of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.