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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 18, Issue 12 - Dec 2008
Volume 18, Issue 11 - Nov 2008
Volume 18, Issue 10 - Oct 2008
Volume 18, Issue 9 - Sep 2008
Volume 18, Issue 8 - Aug 2008
Volume 18, Issue 7 - Jul 2008
Volume 18, Issue 6 - Jun 2008
Volume 18, Issue 5 - May 2008
Volume 18, Issue 4 - Apr 2008
Volume 18, Issue 3 - Mar 2008
Volume 18, Issue 2 - Feb 2008
Volume 18, Issue 1 - Jan 2008
Selecting the target year
Determination of Seed Purity in Radish (Raphanus sativus L.) Using Allozyme
Huh, Man-Kyu ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 907~911
DOI : 10.5352/JLS.2008.18.7.907
Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial
radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 (
), Lap-1 (
), Est-1 (
) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon's index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.
Biological Activity of Recombinant Human Granulocyte Colony-Stimulating Factor and Isolation of the Somatic Cell Transfected EGFP-hG-CSF Gene
Park, Jong-Ju ; Min, Kwan-Sik ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 912~917
DOI : 10.5352/JLS.2008.18.7.912
To investigate the biological activity of recombinant human granulocyte colony-stimulating factor (rec-hG-CSF) in mammalian cells, hG-CSF gene was cloned using the eDNA extracted from the human squamous carcinoma cell lines and rec-hG-CSF was produced in CHO cell lines. To analyze the biological activity in vivo, the rec-hG-CSF protein was injected into mice subcutaneously on days 0 and 2. Blood was withdrawn for white blood cell (WBC) determination 5 days after the first injection. WBC values were found to have increased significantly. A pEGFP-mUII-hG-CSF vector was transfected into somatic cell lines isolated from bovine fetal cells. The colony expressing EGFP signals was observed with a confocal microscope. These data suggest that the rec-hG-CSF produced in this study has potent activity in vivo. Thus, the results of this biological activity show that rec-hG-CSF can be enhanced considerably by genetic engineering that affects potential activity, including mutations, which add the oligosaccharide chain and construct double-fusion proteins. A pEGFP-mUII-hG-CSF vector can be utilized for the production of cloned transgenic livestock.
Identification of Phosphoproteins Induced by AT1 Receptor Blocker Losartan
Lee, Chang-Woo ; Kim, Mi-Jin ; Jang, Sei-Heon ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 918~923
DOI : 10.5352/JLS.2008.18.7.918
The angiotensin II receptor (
) antagonists are effective in treating patients with hypertension and showed beneficial effects in diabetes and other metabolic diseases. The beneficial effects of
antagonists are mainly considered to be from inhibition of Ang
signaling pathway such as the activation of NADPH oxidase and the generation of reactive oxygen species. In this study, we examined whether antagonist of the
could account for phosphorylation of proteins in cells using antibody array. We have selected 6 proteins with Ser/Thr-phosphorylation sites and 12 proteins with Tyr-phosphorylation sites based on literature search. Upon
antagonist losartan treatment to serum-starved COS-1 cells, there was
increase of Ser phosphorylation in small GTPase RhoA. RhoA is known to be responsible for cytoskeleton rearrangement and is down-regulated upon Ser phosphorylation in vivo. Our finding provides a new insight into the mechanism and signaling pathway of the
antagonist in cells.
The Community Structure of Plant at the Edge of the Oncheon River in Busan
Moon, Sung-Gi ; Huh, Man-Kyu ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 924~930
DOI : 10.5352/JLS.2008.18.7.924
Community structure refers to the number of species in a community and the pattern of distribution of individuals among those species. The purpose of this paper was to describe a statistical analysis for detecting a ecological biodiversity which is valid even though the assumption at the different sampling points is not violated spatial randomness of species. Counts and cover were determined from 10 (
) plots in five sites of the Oncheon River which is located in Busan, Korea. Total 95 taxa (85 species, 9 varieties, and one form) were identified and measured in edge sides of this river. These were a total of present in the five sites. Overall across the fragments, mean number of species per plot differed significantly among the five sites (F=7.75, p<0.01). Shannon-Wiener functions differed significantly among plots (F=4.12, p<0.05), with the St. 1 having significantly higher value (2.380) than the others (2.206 for St. 2, 2.116 for St. 3, 2.069 for St. 4, and 0.637 for St. 5). The richness indices, R1 decreased from the upper stream of the Oncheon River to the lower stream. We used a novel way of representing community structure to show that abundance within closely related pairs of co-occurring species in the Oncheon River. The differences between the distributions for of congeners and pairs of non-congeners showed at the largest difference of the cumulative fractions of the data sets (x=0.85).
Responses of Plasma IGF-1, IGFBPs and Hepatic GH Receptor to Growth Hormone Releasing Peptides (GHRP)-2 Administration and Energy Level in Wethers
Lee, Hong-Gu ; Jin, Young-Cheng ; Hidari, Hisashi ; Choi, Yun-Jaie ; Kim, Seon-Ku ; Shin, Teak-Soon ; Cho, Byung-Uuk ; Kim, Yong-Gyun ; Kim, Keun-Ki ; Son, Hong-Joo ; Lee, Sang-Mong ; Park, Hyun-Chul ; Kang, Han-Seok ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 931~939
DOI : 10.5352/JLS.2008.18.7.931
The purpose of this study was to determine the effect of energy supplement on responses of plasma insulin-like growth factor (IGF)-1 and IGF binding proteins (IGFBPs) to growth hormone-releasing peptide-2 (GHRP-2) administration in normal protein-fed wethers, and to observe the effect of GHRP-2 treatment on hepatic growth hormone (GH) receptor in well-fed wethers. Plasma IGF-1 and 39-42 kDa IGFBP-3 during the HENP (CP, crude protein 0.34 and TDN, total digestible nutrients 1.83 kg/day DM, dry matter intake) treatment period were higher than in the LENP (CP 0.32 kg and TDN 0.87 kg/day DM intake) period (P<0.05). The response of GH was stimulated by GHRP-2 (
body weight/day) administration during both of the feed treatment periods (P<0.05). The area under curve (AUC) increment and average concentration of GH (0-180 min) with GHRP-2 administration was higher during HENP treatment than LENP treatment (P<0.01). During the HENP treatment period from day 1 to day 7 of twice daily GHRP-2 treatment, the plasma IGF-1 increment was increased on days 2, 6 and 7 of GHRP-2 administration (P<0.05). On the basis of ligand blotting, the proportions of plasma 39-43 kDa IGFBP-3 during the HENP treatment period only showed a significant difference on days 6 and 7 with GHRP-2 administration. No significant difference in the specific binding of
oGH to hepatic membranes was detected between the saline and GHRP-2 treatments of the HENP-fed wethers. These results suggest that the nutritional balance between energy and protein may affect the endogenous GH / IGF-1 axis as well as plasma IGFBP-3 levels.
(γ-Aminobutyric Acid Transporter 2 Binds to the PDZ Domain of Mammalian Lin-7
Seog, Dae-Hyun ; Moon, II-Soo ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 940~946
DOI : 10.5352/JLS.2008.18.7.940
Neurotransmitter transporters, which remove neurotransmittesr from the synaptic cleft, are regulated by second messenger such as protein kinases and binding proteins. Neuronal
acid transporters (GATs) are responsible for removing the inhibitory neurotransmitter
acid (GABA) from the synaptic cleft.
acid transporters 2 (GAT2/BGT1) is involved in regulating neurotransmitter recycling, but the mechanism how they are stabilized and regulated by the specific binding protein has not yet been elucidated. Here, we used the yeast two-hybrid system to identify the specific binding protein(s) that interacts with the C-terminal region of GAT2 and found a specific interaction with the mammalian LIN-7b (MALS-2). MALS-2 protein bound to the tail region of GAT2 but not to other GAT members in the yeast two-hybrid assay. The "T-X-L" motif at the C-terminal end of GAT2 is essential for interaction with MALS-2. In addition, this protein showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to GAT2 specifically co-immunoprecipitated MALS associated with GAT2 from mouse brain extracts. These results suggest that MALS may stabilize GAT2 in brain.
Effect of Zinc-Enriched Yeast Supplementation on Serum Zinc and Testosterone Concentrations in Ethanol Feeding Rats
Cha, Jae-Young ; Heo, Jin-Sun ; Park, Bo-Kyung ; Cho, Young-Su ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 947~951
DOI : 10.5352/JLS.2008.18.7.947
Zinc is an essential trace element participating in many physiological functions and notably an important function for sperm physiology. Zinc-enriched yeast strain, Saccharomyces cerevisiae, isolated from tropical fruit rambutan. This strain contained 306 ppm zinc concentration and other components contained by K 28,640 ppm, Mg 2,342 ppm, Na 1,048 ppm, Ca 366 ppm, Fe 236 ppm and Mn 4 ppm. The serum concentration of testosterone was decreased in ethanol treatment rats. As compared with ethanol treated control rats, the zinc-enriched yeast strain supplementation showed significantly increased the testosterone concentration in serum. In addition, zinc concentration in serum was decreased in alcohol treatment, but this reduction was significantly increased by zinc-enriched yeast strain supplementation in ethanol feeding rats. These results indicate that zinc-enriched yeast Saccharomyces cerevisiae strain could play an important role in the sperm physiology by the marked elevation of serum testosterone concentration.
Effect of Various Factors on Early THP-1 Cell Adhesion Induced Phorbol 12-Myristate 13-Acetate (PMA)
Jo, Yong-Sam ; Shin, Ji-Hyun ; Choi, Tae-Saeng ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 952~957
DOI : 10.5352/JLS.2008.18.7.952
We evaluated the effects of various factors (e.g., serum, inhibitors of protein synthesis, and cytoskeleton and protein kinases) on early PMA-induced THP-1 cell adhesion using an adhesion assay with Sulforhodamine B (SRB) staining, which was used to assess the proliferation of the attached cells. THP-1 cell adhesion to a plastic substrate was detected 1 hr after exposure to Phorbol 12-Myristate 13-Acetate (PMA) and peaked after 18 hr. At concentrations > 25 nM PMA, the level of adhesion did not change. Based on our preliminary results, we used 25 nM PMA and 5 hr of culture as standard assay conditions. Early PMA-induced cell adhesion was not affected by the presence of serum or PD 98059 in the culture medium, but was affected by the addition of PKC inhibitors and cycloheximide. In the presence of actin inhibitor with PMA, the cell adhesion increased when comparing with PMA treatment only. Thus, early PMA-induced adhesion of THP-1 cells does not require serum in the culture medium, MAP-kinase activation, or actin polymerization, but does require de novo protein synthesis and PKC activation. Our SRB-based cell adhesion assay may be used to screen other PKC inhibitors.
Nutritional Characteristics of Water Extract from Kangaroo Tail.
Ha, Soon-Ok ; Park, In-Hye ; Lee, Yong-Seok ; Kim, Jae-Kyu ; Chung, Soo-Yeol ; Choi, Yong-Lark ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 958~962
DOI : 10.5352/JLS.2008.18.7.958
To get the nutritional data of hot water extract from imported kangaroo tails, research was done about contents of collagen, chondroitin sulfate, protein, fat, mineral ions, and conjugated linoleic acid (CLA) in tails. Collagen content of sulfuric acid digested sample was way higher at bones than meats in both kangaroo tail and cow tail. Comparing kangaroo tail and cow tail, meat of kangaroo tail have 1.7 times higher collagen content than that of cow tail. Content of collagen in bone parts of kangaroo tail was also higher 1.2 times than that of cow tail. Meat sample of kangaroo tail (liquid extraction) have 1.3 times higher content of muco-polysaccharide than that of cow tail. In the born part, kangaroo tail was 2.4 times higher than cow tail in its content of muco-polysaccharide. The CLA content of the kangaroo tail showed the content that was higher than 0.9% of the cow tail for 4.9% and showed about 5.3 times high ratios. Especially in kangaroo tail, a band with high content of CLA was found between C18:1 and C18:2.
Antioxidant Effects and Tyrosinase Inhibition Activity of Oriental Melon (Cucumis melo L. var makuwa Makino) Extracts.
Shin, Yong-Seub ; Lee, Ji-Eun ; Yeon, Il-Kweon ; Do, Han-Woo ; Cheung, Jong-Do ; Kang, Chan-Ku ; Choi, Seng-Yong ; Youn, Sun-Joo ; Cho, Jun-Gu ; Kwoen, Dae-Jun ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 963~967
DOI : 10.5352/JLS.2008.18.7.963
The biological activities of water extracts from different fruit parts, such as peel, flesh, and placenta, of oriental melon were investigated. The concentrations of total phenolic in fruit extracts were
, respectively. Whereas the total flavonoid content in the peel extracts was
, those in the extract of flesh and placenta were
, respectively. The DPPH free radical scavenging activity of each fruit extract at 10,000 ppm was 34.84% for peel, 10.70% for placenta and 9.26% flesh. The ABTS radical cation decolorizing activity of each fruit extract at 10,000 ppm was in fruit extracts were 72.92% for peel, 48.0% for flesh and 74.31% for placenta. In addition, xanthine oxidase inhibitory activity,
inhibition activity, and tyrosinase inhibition activity of the peel extracts appeared to be higher than those of placenta and flesh. Taken together, these results indicated that the peel part of oriental melon contained higher level of total flavonoid content, and several physiological activities including antioxidation,
inhibition activity, and tyrosinase inhibition activity than did the flesh and placenta parts, and suggested that the peel might have a potential to be applicable as a source for functional foods.
The Change of Functional Fitness and Bone Mineral Density on a Long-Term Combined Exercise Intervention in Breast Cancer Survivors.
Kim, Yang-Sook ; Kim, Mi-Sook ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 968~973
DOI : 10.5352/JLS.2008.18.7.968
The study was to provide basic data and to examine the effect of combined exercise for 12 months on functional fitness and bone mineral density (BMD) in breast cancer survivors. The subjects of this study were 40 to 60-year-old married women (N=24) who finished their treatments chemotherapy and radiation therapy. They were divided into two groups that exercise group and exercise with alendronate group. Eighteen (T-score=
) of the 24 women who were diagnosed osteopenia (N=15) and osteoporosis (N=3), participated in combined exercise (EG). The other six (T-score=
) women who were diagnosed as osteoporosis (EDG), participated in the combined exercise program with osteoporosis drug (Alendronate 70 mg/w). The result of the analysis was as follows: Twelve months after, the participants (N=24) had a significant increase of the items such as sit and reach ups, grip strength (R and L) and sit ups test of functional fitness in the periods. In body composition, FM (fat mass) had significant decrease in periods. In the comparison of BMD, EG (N=18) had no change, while EDG (N=6) had significant improvement in L1, T12 and T-score after 12months. Consequently, complex exercise program (Hatha yoga, elastic band, gym ball) had positive effect on functional fitness and bone mineral density. We suggest that complex exercise program can be applied as recovery program after breast cancer surgery. Further research needs various and repetitive studies from more different targets or methods in the exercise program for its improvement.
Antifungal Activity of Zanthoxylium schinifolium Against Fusarium graminearum, a Barley Powdery Mildew Fungus.
Kim, Byum-Soo ; Jang, Han-Su ; Choi, Chung-Sig ; Kim, Jong-Sik ; Kwon, Gi-Seok ; Kwun, In-Sook ; Son, Kun-Ho ; Sohn, Ho-Yong ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 974~979
DOI : 10.5352/JLS.2008.18.7.974
The powdery mildew, a fungal plant disease found in varieties of plant cultures, is occurred by attack with Fusarium sp., Sphaerotheca sp., Leveilluna sp., and Eryshipe sp.. In this study we investigated the control of Fusarium graminearum, a barley powdery mildew fungus, by natural plant extracts. Among the 900 plant extracts tested, Zanthoxylum schinifolium, Ligusticum acutilobum, Bidens frondosa L., Dictamnus dasycarpus, Evodia officinalis, Disporum sessile, Scopolia japonica Max., Styrax japonica S. et Z., Dictamnus dasycarpus Turcz., Sinomenium acutum Rehder et Wils., Eugenia aromaticum, Rubus parvifolius L., Reynoutria elliptica, Coptis chinensis, Paeonia lactiflora Pall., Rheum undalatum, Paeonia suffruticosa, Oenothera odorata Jacq., Euphorbia pekinensis Rupr., and Nepeta cataria were selected based on spore germination inhibition assay. Further mycelial growth inhibition assay with economical and safety considerations led us to finally select Z. schinifolium (sancho) for control of F. graminearum. To produce antifungal sancho extract, methanol was suitable for extraction and subsequent fractionations of the extract showed that the water residue mainly had antifungal activity. The sancho extract and its fractions showed minor antibacterial activity against different pathogenic or food spoilage bacteria, but they did not show any harmful effects against young tomato plant by treatment of
in green chamber test. These results suggested that the extract of sancho has high potentials on control of a powdery mildew fungus, F. graminearum.
Development of an Alcoholic Drink Using Onion Extract.
Kim, Sam-Woong ; Oh, Eun-Hye ; Jun, Hong-Ki ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 980~985
DOI : 10.5352/JLS.2008.18.7.980
This study was carried out to develope an alcoholic drink by fermentation of onion extract using Saccharomyces cerevisiae. The optimal conditions for ethanol production were obtained by standing culture at
for 5 days with 5% inoculum volume. At the results by flask culture, the growth curve of used S. cerevisiae reached to the stantionary phase at 48 hr and the death phase at 90 hr, whereas ethanol production reached maximum at 114 hr. Under the above conditions, a large scale production was carried out. A standing culture in 5 l fermenter showed the similar results to its flask culture, but progressed 24 hr rapidly more than that of the flask culture. A fed-batch culture was performed by addition of the onionic medium supplemented with 10% (v/v) sucrose after 72 hr from the fermenting start. The fed-batch culture could prevent S. cerevisiae from entering into the death phase and maintain constant level of alcohol production. A continuous culture was able to carry out by adding per every 24 hr the onionic medium supplemented with 10% (v/v) sucrose after 72 hr from the fermenting start. Although S. cerevisiae used showed a little decreased growth, alcohol production maintained roughly the constant level at the maximum yield. To enhance the quality of this alcoholic drink,
L-ascorbic acid (AA-2G) was supplemented into the onion extract of the substrate for fermentation. As resulted at this study, this alcoholic drink containing AA-2G should be used as a functional fermented alcohol drink strengthened with vitamin C.
Effects of Taekwondo and Calcium Intake on Physique, Growth Hormone and IGF-1 in Elementary School Male Students.
Lee, Sang-Ho ; Ye, Jeong-Bok ; Baek, Yeong-Ho ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 986~991
DOI : 10.5352/JLS.2008.18.7.986
The purpose of this study was to investigate the effect of Taekwondo and calcium intake on physique, growth hormone and IGF-1 in elementary school male students. 24 subjects who the fourth grade and Taekwondo black belt (
poom) were classified into three groups; Taekwodo +calcium intake group (8), Taekwondo group (8) and control group (8). The period of Taekwondo was 12 weeks, 5 times a week and each time 50 minutes with RPE (
) for intensity were treated and subjects were given calcium at 750 mg of pill after dinner a day during the 12 weeks. The conclusions of this study are as follows; In the comparison within each group, three groups all significantly increased in body height but in the comparison between groups, A and B groups were more significantly increased in body height than C. C significantly decreased in growth hormone and in the comparison between groups, A and B groups were more significantly increased in growth hormone than C. In the comparison within each group, A and B significantly increased in IGF-1 and in the comparison between groups, A and B groups were more significantly increased in IGF-1 than C. It is considered that Taekwondo and calcium intake give effects to body height by increasing growth hormone and IGF-1. It is necessary to continue the study, because the study results of growth hormone and IGF-1 are various by training method, intensity, nutrition.
Establishment of the Extraction Process by Evaluation of Proanthocyanidin Contents and Antioxidative Activities of Pine (Pinus densiflora) Needle Extracts.
Lee, Ok-Hee ; Kim, Ki-Young ; Jang, Min-Kyung ; Yu, Ki-Hwan ; Yoo, Byung-Hong ; Kim, Sung-Gu ; Kim, Mi-Hyang ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 992~998
DOI : 10.5352/JLS.2008.18.7.992
We evaluated proanthocyanidin contents out of total polyphenolic compounds in two pine needle extracts prepared by combined extraction methods and compared their antioxidative activities. Amounts of total polyphenolic compounds of sub-supercritical hotwater precipitate (SSHP) and sub-supercritical hotwater supermatant (SSHS) were 225.4 and 116.6 mg/g, respectively. Amounts of proanthocyanidin of SSHP and SSHS were 9.9 and 3.6 mg/g, respectively. Thus, the ratio of proanthocyanidin contents over total polyphenolic compounds of SSHP and SSHS were 4.3 and 3.9%, respectively. Antioxidative activities (
) of SSHP and SSHS measured by DPPH radical scavenging were 41.5 and
, respectively. These results indicates that two combined extracts, SSHP and SSHS showed higher antioxidative activities than extract prepared by hot water or sub-supercritical. In conclusion, extraction procedures for SSHP and SSHS could be useful methods for preparing the effective components of functional food products.
Effect of Bulnesia sarmienti Water Extracts on Lipid Metabolism in Neonatally Streptozotocin- Induced Diabetic Rats.
Park, Chang-Ho ; Lee, Gee-Dong ; Kim, Jung-Ok ; Kim, Kil-Soo ; Lee, Won-Young ; Hong, Joo-Heon ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 999~1004
DOI : 10.5352/JLS.2008.18.7.999
The effects of Bulnesia sarmienti water extract on lipid metabolism were investigated in type-2 diabetes rats. The rats were fed a basal diet and B. sarmienti water extract for 8 weeks. The rats were randomly assigned to each treatment group : normal, control, two kinds of treatment group (BW1: 0.9 mg/ml, BW2: 1.8 mg/ml). After 8 weeks of experimental diets consumption, the body weights and food efficiency ratio of B. sarmienti water extract fed groups were not different from those in the control group. The concentrations of total-cholesterol, LDL-cholesterol, and triglyceride in blood plasma were significantly decreased in the B. sarmienti water extract groups comparison to the control group. Although blood glucose control effects were not observed, the B. sarmienti water extract feeds were highly effective in lowering the levels of blood lipid substances such as triglycerides, total-cholesterol, and LDL-cholesterol.
Inhibitory Effects of Four Solvent Fractions of Alnus firma on α-Amylase and α-Glucosidase.
Choi, Hye-Jung ; Jeong, Yong-Kee ; Kang, Dae-Ook ; Joo, Woo-Hong ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 1005~1010
DOI : 10.5352/JLS.2008.18.7.1005
In this study, we investigated the inhibitory effect of four solvent fractions of Alnus firma on
and aldose reductase activities. The inhibitory test showed that methanol (MeOH) extract and hexane (HX) fraction strongly inhibited pork pancreatin and salivary
activity. The MeOH extract and HX fraction of Alnus firma at the concentration of 4 mg/ml inhibited more than 70% of pancreatin and salivary
activity. The inhibitory effect of fractions has different specificities against
from pancreatin and salivary. In addition, the MeOH extract and butanol (BuOH) fraction showed the highest inhibitory activity on yeast
at values of
respectively. The MeOH extract and BuOH fraction showed the highest inhibitory activity on yeast
than commercial agent such as 1-deoxynorjirimycin and acarbose. Inhibition kinetics of solvent fractions showed that
has been inhibited noncompetitively by the MeOH, EA and BuOH fraction. The aldose reductase from human muscle cell had been inhibited strongly by the MeOH extract and EA fraction at 57.996% and 83.293% at the concentration of
, respectively. These findings may contribute to biological significance in that
and aldose reductase inhibitory compounds could be used as a functional food and a drug for the symptomatic treatment of antidiabetic disease in the future.
Genetic Factor of Bitter Taste Perception in Humans.
Lee, Hye-Jin ; Kim, Un-Kyung ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 1011~1014
DOI : 10.5352/JLS.2008.18.7.1011
The ability or inability to taste phenylthiocarbamide (PTC) is a classic inherited trait that has been best-studied in human populations. Also, variation in PTC perception has been correlated with dietary preferences and thus may have important consequence for diet-related diseases in modem populations. The recent identification of the TAS2R38 gene (PTC gene) which is a member of TAS2R family of bitter taste receptor genes and three common polymorphisms in the gene is highly correlated with taste sensitivity to PTC. Balancing natural selection has acted to maintain high frequency of both alleles of the gene in human population. Future detailed studies of the relationships between molecular mechanisms and taste function may have therapeutic implications, such as helping patients to consume beneficial bitter-tasting compounds.
The Population Genetic Structure of the Oyster Crassostera gigas (Bivalvia:Ostreidae) from Gamak Bay in Korea
Cho, Eun-Seob ; Jeong, Hee-Dong ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 1015~1018
DOI : 10.5352/JLS.2008.18.7.1015
To analyze the population genetic structure of the oyster Crassostrea gigas Thunberg, 34 specimens werecollected from Gamak bay in March, 2007. Total genomic DNA was extracted from each sample and PCR was performed to identify haplotypes of oyster by using HCO2918 and LCO1491 primers. Four kinds of haplotypes (CR1, CR2, CR3, and CR4) were identified. Among these group, CR3 showed the highest relative frequency at 73% than any other of haplotypes. On the basis of hierarchical genetic structure, the population of Gamak showed a higher genetic relationship with Namhae, but the genetic distance between southern and western coasts was negative and no statistical significance was found (p>0.05). Consequently, the oyster from Korea coast is determined to be both homogenous and large.
Chromosome Compositions of Four Cultivated Cucurbitaceae Species.
Kwon, Ji-Yeon ; Park, Hye-Mi ; Lee, Sung-Nam ; Choi, Sun-Hee ; Song, Kyung-A ; Kim, Hyun-Hee ;
Journal of Life Science, volume 18, issue 7, 2008, Pages 1019~1022
DOI : 10.5352/JLS.2008.18.7.1019
The chromosome numbers and compositions were investigated in four cultivated species of Cucurbitaceae; Cucumis sativus L., Citrullus lanatus (Thunb.) Matsum. et Nakai, Cucumis melo L., Luffa cylindrica (L.) Roemer. through general aceto-orcein staining method. The chromosome compositions of four species were diploids of 2n=22, 2n=24 and 2n=26 respectively. The chromosomes were relatively small and showed gradual length degradation from
in Cucumis sativus,
in Cucumis melo,
in Citrullus lanatus and
in Luffa cylindrica. The chromosome types consisted of all metacentrics in Cucumis sativus, seven pairs submetacentrics and five pairs metacertrics in C. melo, four pairs of submetacentrics and seven pairs metacertrics in Citrullus lanatus, and two pairs submetacentrics and eleven pairs metacentrics in Luffa cylindrica (L.) Roemer.. The satellites were found in a pair of chromosomes in C. melo and two pairs in Luffa cylindrica. The chromosome compositions in these four species showed species-specific patterns and seemed to provide useful informations for breeding and molecular cytogenetic works on Cucurbitaceae.