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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 19, Issue 12 - Dec 2009
Volume 19, Issue 11 - Nov 2009
Volume 19, Issue 10 - Oct 2009
Volume 19, Issue 9 - Sep 2009
Volume 19, Issue 8 - Aug 2009
Volume 19, Issue 7 - Jul 2009
Volume 19, Issue 6 - Jun 2009
Volume 19, Issue 5 - May 2009
Volume 19, Issue 4 - Apr 2009
Volume 19, Issue 3 - Mar 2009
Volume 19, Issue 2 - Feb 2009
Volume 19, Issue 1 - Jan 2009
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Involvement of Cdk Inhibitor p21(WIP1/CIP1) in G2/M Arrest of Human Myeloid Leukemia U937 Cells by N-Methyl-N'-Nitro-N-Nitrosoguanidine
Choi, Yung-Hyun ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 1~8
DOI : 10.5352/JLS.2009.19.1.001
In this paper, to elucidate the further mechanisms of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced growth arrest, we investigated the effect of MNNG on cell cycle and proliferation in U937 cells, a p53-null human myeloid leukemia cell line. It was found that MNNG causes an arrest at the G2/M phase of the cell cycle and induces apoptosis, which is closely correlated to inhibition of cyclin B1 and cyelin-dependent kinase (Cdk) 2-associated kinase activities. MNNG treatment in. creased protein and mRNA levels of the Cdk inhibitor p21(WAF1/CIP1), and activated the reporter construct of a p21 promoter. By using p21 promoter deletion constructs, the MNNG-responsive element was mapped to a region between 113 and 61 relative to the transcription start site. These data indicate that in U937 cells MNNG can circumvent the loss of wild-type p53 function and induce critical downstream regulatory events leading to transcriptional activation of p21. Present results indicate that the p53-independent up-regulation of p21 by MNNG is likely responsible for the inhibition of cyclin/Cdk complex kinase activity rather than the down-regulation of cyclins and Cdks expression. These novel phenomena have not been previously described and provide important new insights into the possible biological effects of MNNG.
Screening and Purification of an Anti-Prostate Cancer Compound, Deoxypodophyllotoxin, from Anthriscus sylvestris Hoffm
Cho, Hyo-Jin ; Yu, Sun-Nyoung ; Kim, Kwang-Youn ; Sohn, Jae-Hak ; Oh, Hyun-Cheol ; Ahn, Soon-Cheol ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 9~14
DOI : 10.5352/JLS.2009.19.1.009
The prostate cancer is the critical health problem, increasing of its related death in worldwide. Unfortunately present surgery and chemotherapeutic choices seem to be impossible in curing or controlling prostate cancer, because metastasis occasionally advances even after these potentially curative therapies. Therefore, there is immediate need to alternative chemoprevention and chemotherapeutic agents. Over one hundred species of dried medicinal herbs were tested for proliferation inhibitory effects on prostate cancer cell line, PC-3. One of them, Anthriscus sylvestris was selected because of potent anti-proliferation effect. The dried root of A. sylvestris was extracted with 100% methanol for 2-3 days and its extract was fractionated by using ethyl acetate. And ethyl acetate layer was subjected to column chromatographies on silica gel, reverse phase-18 (RP-18) and Sephadex LH-20, in turn. Finally, the pure compound was obtained by crystallization in methanol at
for overnight and identified as deoxypodophyllotoxin by NMR spedorscopic and physico-chemical analyses. In addition, it was confirmed that deoxypodophyllotoxin clearly inhibits the proliferation of PC-3 cells in a dose and time-dependent manner.
De novo Expression of Hepatic UCP3 Is Time-Dependently Related with Metabolic Function in Fenofibrate-Treated High Fat Diet Rats
Park, Mi-Kyoung ; Kang, Ah-Young ; Seo, Eun-Hui ; Joe, Yeon-Soo ; Kang, Soo-Jeong ; Hong, Sook-Hee ; Kim, Duk-Kyu ; Lee, Hye-Jeong ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 15~20
DOI : 10.5352/JLS.2009.19.1.015
Uncoupling protein 3 (UCP3) is a mitochondrial protein that is expressed predominantly in skeletal muscle. It may play a role in altering metabolic function. However, its major physiological roles are not fully understood. Recently de novo expression of UCP3 in rat liver by fenofibrate was reported. We also reported previously that fenofibrate-induced de novo expression of UCP3 contributes to reduction of adipose tissue in obese rats. In the present study, we investigated that ienofibrate-induced expression of UCP3 in rat liver is related with metabolic function such as body weight and hepatic lipid content by time-dependent manner in high-fat diet rats. Eight-week-old male Sprague-Dawley rats were randomly divided into two groups; the high fat diet group (HF, n=16) and fenofibrate-treated high fat diet group (HFF, n=16). The mRNA expression of hepatic UCP3 was detected as early as 1 week of fenofibrate treatment by quantitative real-time PCR and the amount of mRNA was increased time-dependently. The mean body weight of the HFF group was significantly less com. pared with the HF group after 6 weeks of fenofibrate treatment, even though there was no difference of food intake between the two groups. Rectal temperature was increased during 4 to 6 weeks of fenofibrate treatment and body weight was decreased after 6 weeks of treatment. These results were corresponded with the increased amount of the expression of UCP3 mRNA and protein. We suggest that de novo expression of hepatic UCP3 is increased time-dependently with fenofibrate treatment and that the amount of expression is correlated with metabolic function.
Microarray Analysis of Gene Expression by Rhei Rhizoma Water Extracts in a Hypoxia Model of Cultured Neurons
Lee, Hyun-Sook ; Song, Jin-Young ; Moon, Il-Soo ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 21~33
DOI : 10.5352/JLS.2009.19.1.021
In this study, we investigated the effect of Rhei Rhizoma (RR; 大黃) water extract on gene expression in a hypoxia model of cultured rat hippocampal neurons. RR water extract
was added to the culture media on day 10 in vitro (DIV10), and a hypoxic shock (2%
, 3 h) was given on DIV13. After maintaining the cultures in normoxia for 24 hr, total RNA was isolated and used for microarray analysis. The MA-plot indicated that most genes were up- or downregulated within 2-fold. There were more downregulated genes (725 ea) than upregulated ones (472 ea) when larger than Global M value 0.2 (i.e., >15% increase) or smaller than Global M value -0.2 (i.e., >15% decrease) were considered. Antiapoptosis genes such as Tegt (2.4-fold), Nfkb1 (2.4-fold) Veg (1.8-fold), Ngfr (1.6-fold) were upregulated, while pro-apoptosis genes such as Bad (-64%), Cstb (-66%) were downregulated. Genes for combating environmental stress (stress response genes) such as Defb3 (2.7-fold), Cygb (2.2-fold), Ahsg (2.18-fold), Alox5 (2-fold) were upregulated. Genes for cell proliferation (cell cycle-related genes) such as Erbb2 (1.84-fold), Mapk12 gene (1.8-fold) was upregulated. Therefore, RR water extracts upregulate many pro-survival genes while downregulating many pro-death genes. It is interpreted that these genes, in combination with other regulated genes, can promote neuronal survival in a stress such as hypoxia.
A Simple Method for Isolation of Polymannuronate and Polyguluronate from Alginate Hydrolyzed by Organic Acids
Lee, Dong-Soo ; Shin, Myung-Kyo ; Pyeun, Jae-Hyeung ; Lee, Jin-Woo ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 34~39
DOI : 10.5352/JLS.2009.19.1.034
Alginate with a MW of 1,283 kDa was hydrolyzed with 0.4 M organic acids at
for 3 hr. Molecular weights of alginates hydrolyzed with organic acids ranged from 7.5 to 53.2 kDa. There was no significant difference in the molar ratio of mannuronate to guluronate in alginates hydrolyzed with organic acids. Acetic add was found to be the most effective organic acid for hydrolysis of alginate. The MW of alginate decreased with increasing concentration and reaction time with acetic acid as a hydrolyzing agent. The correlations between the MW of hydrolyzed alginate and concentration of acetic acid as well as reaction time with 0.4 M acetic acid were plotted and the relevant equations obtained in this study. Polymannuronate and polyguluronate were isolated by pH adjustment of alginate hydrolyzed with 0.4 M acetic add. The molar percentages of mannuronate in polymannuronates isolated from alginate hydrolyzed with 0.4 M acetic acid at
were increasing in proportional to the reaction time such as 75% for 1 hr, 90% for 3 hr, and 98% for 5 hr of reaction time.
Antidiabetic Effects of Leaves Extracts of Psidium guajava L. and Lagerstroemia speciosa L. in STZ-induced Rats
Roh, Sang-Geun ; Kim, Kyun-Ha ; Choi, Won-Chul ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 40~45
DOI : 10.5352/JLS.2009.19.1.040
Guava (Psidium guajava L.) and banaba (Lagerstroemia speciosa L.) are well known as medicinal plants for their antidiabetic effects. These contain a great deal of polyphenol compound and work on the treatment of diabetes mellitus effectively. In this study, the extracts of guava and banaba are consumed by streptozotocin (STZ) induced diabetic rats to compare the antidiabetic effects. According to the comparison result, the glucose level of those STZ-induced diabetic rats has decreased by 19.32%, total cholesterol by 24-46%, triglyceride by 22-67% and free fatty acid by 49-71 % approximately compared to the diabetic rats, while the generation of insulin and the recovery of beta cells have increased. However, the result showed that the antidiabetic effect of guava extracts was higher than that of banaba extracts. This is because the hydrophilic polyphenol compounds contained in banaba leaves were not extracted during the ethanol extraction process, and the antidiabetic activity of the extracted corosolic add was low to surprise.
The Effect of Different Triathlon on Weight, Sodium and Hematological Changes
Park, Chan-Ho ; Kim, Tae-Un ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 46~51
DOI : 10.5352/JLS.2009.19.1.046
This study examined how differing triathlon durations effect weight, serum sodium concentration, and hematological levels, and investigated relationships between these variables and finishing time as well as between body weight changes and serum sodium following Ironman distance triathlons (IDT) and Olympic distance triathlons (ODT). All eight athletes successfully completed ODT and IOT. We found that the mean body weight decreased following both IDT and ODT and that serum sodium was significantly (p<0.05) increased immediately after IDT, RBC, Hb, Hct, and MCH were significantly (p<0.05) increased immediately after IDT. Hct was significantly (p<0.05) decreased immediately after ODT. MCHC was significantly (p<0.01) increased immediate1y after ODT. In IDT, the percentage of change in body weight correlated to the percentage of change in serum sodium concentrations (r=0.75, p=0.05). In conclusion, our srudy showed that serum sodium concentraion increased in IDT and maintained in ODT, despite significant body weight loss during the races. The percentage of change in body weight was related to serum sodium concentration but unrelated to performance in the triathlon. The changes of RBC, Hb, and Hct values from two different length triathlons were depending on race distance.
The Antioxidative and Antimicrobial Effects of Celastrus orbiculatus
Kang, Dae-Yeon ; Shin, Mi-Ok ; Shon, Jae-Hak ; Bae, Song-Ja ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 52~57
DOI : 10.5352/JLS.2009.19.1.052
This study was carried out to investigate the antioxidative and antimicrobial activities of Celastrus orbiculatus (CO). CO was extracted with methanol and then further fractionated with n-hexane (COMH), butanol (COMB), methanol (COMM) or aqueous (COMA) to get active fractions. The antioxidative activity of fractions from CO was investigated by measuring the scavenging activities of CO against DPPH radical, peroxynitrite (
) and reactive oxygen speicies (ROS). Among the various solvent fractions, the COMB showed a marked scavenging effect against DPPH radical, peroxynitrite (
) and reactive oxygen species(ROS). The antimicrobial activity was increased in proportion to its concentration by the paper disk method. Among the various solvent fractions, the COMM and COMB fractions of CO showed strong antimicrobial activities. The results suggest that The CO may be suitable for development as a food preservative and alternative antioxidant.
Molting Patterns of Flight Feathers of Immature Steller's Sea Eagle(Haliaeetus pelagicus) Raised in Captivity
Kang, Seung-Gu ; Lee, In-Sup ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 58~64
DOI : 10.5352/JLS.2009.19.1.058
This study was conducted to know the molting sequence and the aging points of flight feathers of steller's sea eagles (Haliaeetus pelagicus). For this study, two captive immature steller's sea eagles raised at the Ornithology Laboratory attached to Kyungsung University were surveyed for five years from Nov. 2000 to Nov. 2005. The survey indicated that the first molting began in July of the second year, and the primaries of P1-3, the secondaries of S18-19 (female), S17-18 (male), and S1 and S4 were replaced by one-time with second generation feathers. Generally molting stopped during the winter period, but a few feathers continued to molt during the winter. The two secondaries of S18-19 (female) and S17-18 (male) always molted every year but some of the juvenile secondaries (male: S10, S11, etc) retained for 2 or 3 years. In the molting order of primaries, the first molting started at P1 and it proceeded to P10 of outside. In the secondaries, the first molting started at S17(male) and S19(female), and it proceeded to outside. After that molting it started at S1 and proceeded to inside. In the other secondaries, the pattern of molting which proceeded in the mid-part of the secondaries was usually beginning in several different points at the same time. The molting seemed as if it depends on both the conditions of the individuals and the environment, so it was very difficult to explain the molting pattern in the mid-part of the secondaries. The longer quills (P7, P8) required for more than 68 days to develop. In the comparison of the length in the remiges between the first and the second generation feathers, the first generation feathers were the larger than that of the second. And the reduction of the length between the second and the third generation feathers was a few. The reduction of the length between the third and the fourth generation feathers was slight. The juvenile primaries were dark brown with a whitish base, which could be observed until the second or the third generation feathers (in their third or fourth winter plumage).
Effects of Steam Flaking of Corns imported from USA and India on the in vitro Fermentation Characteristic and the Mycotixin Contents of Logistic Processing Line
Lee, Shin-Ja ; Lee, Ji-Hun ; Shin, Nyeon-Hak ; Han, Jung-Hun ; Hyun, Jong-Hwan ; Moon, Yea-Hwang ; Lee, Sung-Sill ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 65~74
DOI : 10.5352/JLS.2009.19.1.065
The objective of this study was to examine the effects of steam flaking treatment of corn grains imported from USA and India on in vitro gas production, microbial growth and contents of aflatoxin
and ochratoxin A. Each treatment was composed of total 4 treatments including (1) USCW (US com-whole type), (2) USCF (US corn-flaked type), (3) IDCW (India corn-whole type) and (4) IDCF (India orn-flaked type) with 4 replications
6 incubation times (3, 6, 9, 12, 18 and 24 hr). Mycotoxin (aflatoxin
& ochratoxin A) contents in test corns tended to increase gradually with increasing logistics periods from the harbor, hopper, silo to processing line. The contents of aflatoxin
in India corn (IDCW) and US corn (USCW) were 11.71 and 1.78 ppb, respectively when measured at the hopper. After steam flaking, both contents of aflatoxin
in USCW and IDCW were 0.00 ppb. It means that Aspergillus flavus could be decreased by steam flaking. However, this trend was not observed in ochratoxin content. The gas production rate of USA corns (USCW & USCF) was significantly (p<0.05) higher than India corns (IDCW & IDCF), and that of steam flaked corns (USCF & IDCF) was higher
than whole corns (USCW & IDCW) after 3 hr incubation in in vitro experiment. pH value was optimally maintained for microbial growth during whole incubation times with the value of 6.05 to 6.54, and was not significantly different between treatments, but USCF was somewhat lower than other treatments. pH value decreased following 12 hr of incubation but gas production increased rapidly during the same period. In addition, in vitro microbial growth rates also increased with up to 18 hr of incubation period, thereafter experienced a decrease with extended incubation time. In conclusion, US corn was superior to India corn by origin based on the results of in vitro and mycotoxin contents. And steam flaking process of imported corns tended to decrease mycotoxin contents such as aflatoxin
and ochratoxin A as well as improve in vitro gas production and microbial growth rates.
Inhibitory Effects of Fucoidan on Melanin Synthesis and Tyrosinase Activity
Jung, Sook-Hee ; Ku, Mi-Jung ; Moon, Hee-Jung ; Yu, Byeng-Chul ; Jeon, Man-Joong ; Lee, Yong-Hwan ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 75~80
DOI : 10.5352/JLS.2009.19.1.075
Melanogenesis is a physiological process that results in the synthesis of melanin pigments. Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. Among the possible melanin-reducing compounds, tyrosinase inhibitors are most promising for preventing and treating pigmentation disorder and are used as skin-whitening agents in the cosmetic industry. In the present study, the effects of fucoidan on melanogenesis and tyrosinase activity of B16F10 melanoma cells were investigated. Melanin synthesis and tyrosinase activity in B16F10 melanoma cells were decreased in a dose-dependent manner by fucoidan. Melanin production and tyrosinase activity in B16F10 melanoma cells stimulated by a-melanocyte stimulating hormone (a-MSH) were inhibited by fucoidan with a dose-dependent manner compared to control. Fucoidan inhibited tyrosinase activity of B16F10 melanoma cells with a dose-dependent manner as assessed by 3,4-dihydroxyphenylalanine (DOPA) staining. In conclusion, these findings indicate that fucoidan, which inhibit melanin synthesis and tyrosinase activity, is an effective skin-whitening agent.
Effects of 12 Week Regular Aerobic Exercise on ST-segment and QTc Interval in Type 2 Diabetes Mellitus Patients
Kim, Young-Il ; Paik, Il-Young ; Jin, Hwa-Eun ; Suh, Ah-Ram ; Kwak, Yi-Sub ; Woo, Jin-Hee ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 81~86
DOI : 10.5352/JLS.2009.19.1.081
The purpose of the present study was to examine effect of 12 week regular aerobic exercise on ST-segment and QTc interval in middle age type 2 diabetes mellitus (T2DM) patients. The subjects consist of 13 type 2 diabetes mellitus (T2DM) patients in middle age men and all of them had no other complications. Subjects participated in aerobic exercise training for 12 weeks. They started to exercise for
, (exercise intensity has been increased gradually) per day,
times a week. The results were compared before and after. Weight and BMI, % body fat, fasting glucose, HOMA-IR,
were significantly decreased and
, exercisre time were significantly increased after 12 week aerobic exercise. Also, QTc interval and ST-segment were significantly decreased during at rest, peak exercise after 12 week aerobic exercise. Conclusionally, 12 week aerobic exercise may be improvement in decreased cardiovascular mortality factors (ST-segment) and abnormal autonomic dysfunction (QTc interval) and potentially increased exercise capacity.
Effects of the Culture Broth of Lactic Acid Bacteria Cultured in Herb Extracts on Growth Promotion and Nonspecific Immune Responses of Aquacultured Fish
Jhon, Bong-Kun ; Kim, Man-Chul ; Kim, Young-Hoo ; Heo, Moon-Soo ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 87~93
DOI : 10.5352/JLS.2009.19.1.087
We have investigated the effects of the culture broth of lactic acid bacteria (LAB) cultured in herb extract on growth, hematological parameter, nonspecific immune responses and disease resistance of olive flounder (Paralichthys olivacells) and parrot fish (Oplegnathus fasciatus) for 12 weeks. Weight gain of olive flounder fed diet with mixture was not significant among the control group. But In parrot fish, was significantly higher 20g than control group. The feed efficiency of olive flounder were 25% higher in the experimental groups than in the control. There were no significant differences in feed efficiency among each group on parrot fish. Treatment of olive flounder contents of GOT and GPT in serum decreased after 8 weeks. But there were no significant differences in GLU and TP among each group. Also, there was no significant of NBT reduction. The activities of lysozyme were higher in experimental group of olive flounder than in the control after 8 weeks. On the other hand, activities of lysozyme were triple higher in the experimental group of parrot fish than in the control after 12 weeks. In the oliver flounder case, the survival rate (%) after an artificial challenge with
CFU/ml of Vibrio anguillarum and Streptococcus iniae per fish, was 18% higher in the experimental groups than the control. The higher survival rate of parrot fish were 17% and 16% in the experimental groups than the control respectively.
Anti-microbial Activity of Grapefruit Seed Extract and Processed Sulfur Solution against Human Skin Pathogens
Ha, Yu-Mi ; Lee, Bo-Bae ; Bae, Hee-Jung ; Je, Kyoung-Mo ; Kim, Soon-Rae ; Choi, Jae-Suk ; Choi, In-Soon ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 94~100
DOI : 10.5352/JLS.2009.19.1.094
This study was carried out to examine the antimicrobial effects of grapefruit seed extract (GSE) and processed sulfur solution (PSS) against human skin pathogens: Malassezia furfur, M. restricta, Propionibacterium arnes, Trichophyton mentagrophytes and T. rubrum. The antimicrobial effects of GSE and PSS were tested by agar diffusion method and micro broth dilution method. As the results, the MIC values of GSE against M. furfur, M. restricta, P. acnes, T. mentagrophytes and T. rubrum were 3.91, 3.91, 0.004, 0.024, and
, respectively. The MIC values of PSS were 0.03, 0.03, 0.156, 0.003, and
, respectively. Antimicrobial activity of skin care emulsion products containing 0.5% GSE and 0.5% PSS against human skin pathogens were 5.2, 4.3, 8.0, 9.5 and 12.8 mm, respectively. Refractive index, pH, viscosity and color value of skin care emulsions containing GSE and PSS were measured. According to these results, it was concluded that the GSE and PSS were the promising sources of antibacterial agent which could be useful for skin and hair care products as well as for the alternative medicine development in treatment of certain types of skin ailments.
Intracullular Functions of the mas2
Gene in the Fission Yeast, Schizosaccharomyces pombe
Sin, Sang-Min ; Cha, Jae-Young ; Ha, Se-Eun ; Sim, Sun-Mi ; Kim, Hyoung-Do ; Lee, Jung-Sup ; Park, Jong-Kun ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 101~110
DOI : 10.5352/JLS.2009.19.1.101
The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel
(mitosis associated protein) gene, a homolog of human SMARCAD1 was isolated and characterized from a fission yeast Schizosaccharomyces pombe (S. pombe) using gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 922 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that an SNF2 domain is located, which is involved in the chromosome remodeling. The quantitative analysis of the
showed that the expression level of
is high before septum formation in S. pombe. When
null mutant cells were grown at 27 and
, the cytokinesis of
null mutant was greatly delayed and a large number of multi-septate and mis-segregated cells were produced. In addition, the number of multi-septate cells significantly increased. When cells were cultured in YES rich medium to increase proliferation, the abnormal phenotypes
null mutant dramatically increased. These phenotypes could be rescued by an over-expression of the mast gene. The Mas2 protein localized in the nuclei of S. pombe, as evidenced by Mas2-EGFP signals. These results suggest that the
is homologous to human SMARCAD1 gene and involved in septum formation and chromosome remodeling control.
The Effects of Rope Jumping and Swiss Ball Exercise on Young Children's Bone Formation Markers
Kim, Tae-Un ; Lee, Kyung-Hee ; Jeon, Jae-Young ; Jeong, Seong-In ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 111~116
DOI : 10.5352/JLS.2009.19.1.111
The purpose of this study was to elucidate the effects of 12 weeks rope jumping and Swiss ball exercise (55 to 75% HRR: 3 times a week) on body composition and bone formation markers in young children. Forty eight participants were divided into two groups, the exercise group (n=24) and the control group (n=24). All items were assesed before and after exercise program. The results of this study are as follows; 1. Body composition: In the comparison of mean changes before and after exercise program execution on body composition within each group, the exercise group showed significant increases in height, weight, fat mass and %fat. The control group showed significant increases in all items. The interaction effect between groups and time didn't appear a significant difference. 2. Osteocalcin: In the comparison of mean changes before and after exercise program execution in osteocalcin within each group, the exercise group didn't appear a significant change and the control group were significantly decreased in girl children. The interaction effect between groups and time didn't appear a significant difference. 3. Alkaline phosphatase: In the comparison of mean changes before and after exercise program in Alkaline phosphatase within each group didn't appear a significant difference in exercise group and control group. The interaction effect between groups and time showed a significant difference. In conclusion, for young children in the stage of the formation of bone mineral contents, rope jumping and Swiss ball exercise are postive effect in promoting their growth and bone health, but osteocalcin concentration did not changes. Thus, further research is required to consider intensity and duration of exercise on bone formation markers of young children's.
Antioxidative Effect of Angelica acutiloba Kitagawa Ethanol Extract
Kim, Ah-Ra ; Lee, Jae-Joon ; Lee, Myung-Yul ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 117~122
DOI : 10.5352/JLS.2009.19.1.117
This study was conducted to investigate the antioxidant effect of 80% ethanol extracts from Angelica acutiloba Kitagawa (A. acutiloba Kitagawa) in vitro. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, n-butanol and water. Antioxidative activities of different fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical generation, Rancimat test, thiobarbituric acid (TBA) value, nitrite scavenging activity, inhibition of lipid peroxidation and peroxide value (POV) in linoleic acid in comparison with the commercial antioxidant butylated hydroxy toluene (BHT). Antioxidant activities of n-hexane fraction of Angelico acutiloba Kitagawa ethanol extract were the highest among fractions and were a little less than that of BHT. Nitrite scavenging activity showed the most remarkable effect at pH 12. These results suggest that ethanol extracts of A. acutiloba Kitagawa can be used in natural antioxidant source.
Multiple Monoclonal Antibodies Produced in a Single Transgenic Plant
Ahn, Mi-Hyun ; Oh, Eun-Yi ; Song, Mi-Ra ; Lu, Zhe ; Kim, Hyun-Soon ; Joung, Hyouk ; Ko, Ki-Sung ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 123~128
DOI : 10.5352/JLS.2009.19.1.123
Production of highly valuable immunotherapeutic proteins such as monoclonal antibodies and vaccines using plant biotechnology and genetic engineering has been studied as a popular research field. Plant expression system for mass production of such useful recombinant therapeutic proteins has several advantages over other existing expression systems with economical and safety issues. Immunotherapy of multiple monoclonal antibodies, which can recognize multiple targeting including specific proteins and their glycans highly expressed on the surface of cancer cells, can be an efficient treatment compared to a single targeting immunotherapy using a single antibody. In this study, we have established plant production system to express two different targeting monoclonal antibodies in a single transgenic plant through crossing fertilization between two different transgenic plants expressing anti-colorectal cancer mAbCO17-1A and anti-breast cancer mAbBR55, respectively. The F1 seedlings were obtained cross fertilization between the two transgenic parental plants. The presence, transcription, and protein expression of heavy chain (HC) and light chain (LC) genes of both mAbs in the seedlings were investigated by PCR, RT-PCR, and immunoblot analyses, respectively. Among all the seedlings, some seedlings did not carry or transcribe the HC and LC genes of both mAbs. Thus, the seedlings with presence and transcription of HC and LC genes of both mAbs were selected, and the selected seedlings were confirmed to have relatively stronger density of HC and LC protein bands compared to the transgenic plant expressing only each mAb. These results indicate that the F1 seedling plant with carrying both mAb genes was established. Taken together, plant crossing fertilization can be applied to generate an efficient production system expressing multiple monoclonal antibodies for immunotherapy in a single plant.
Functional Mechanism of Calmodulin for Cellular Responses in Plants
Cho, Eun-Kyung ; Choi, Young-Ju ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 129~137
DOI : 10.5352/JLS.2009.19.1.129
) plays pivotal roles as an intracellular second messenger in response to a variety of stimuli, including light, abiotic. and biotic stresses and hormones.
-binding protein known to function in transducing signals by activating specific targets and pathways. Among
-binding proteins, calmodulin (CaM) has been well reported to regulate the activity of down-stream target proteins in plants and animals. Especially plants possess multiple CaM genes and many CaM target proteins, including unique protein kinases and transcription factors. Thus, plants are possible to perceive different signals from their surroundings and adapt to the changing environment. However, the function of most of CaM or CaM-related proteins have been remained uncharacterized and unknown. Hence, a better understanding of the function of these proteins will help in deciphering their roles in plant growth, development and response to environmental stimuli. This review focuses on
-CaM messenger system, CaM-associated proteins and their role in responses to external stimuli of both abiotic and biotic stresses in plants.
Biological Constituents of Aged Garlic Extract as Biomarker
Yang, Seung-Taek ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 138~146
DOI : 10.5352/JLS.2009.19.1.138
Garlic (Allium sativum) are an agronomically important genus because of their sulfur flavour components. The majority of the volatiles flavour principles are generated through the enzymatic hydrolysis of the non-volatile organosulfur compounds. However, these compounds may be possible sources of new novel bioacuve and therapeutic principles. Garlic has strong antioxidant activity, and epidemiological studies support the fad that diets rich of garlic may prevent some of the chronic diseases. The health cares of garlic likely arise from a wide variety of components, which may work synergistically. The chemical changes of garlic composition makes it plausible that a variation in processing can lead to acquisition of differential chemical compositions of garlic products. Especially highly unstable allicin can easily disappear during processing and are quickly transformed into a various organosulfur compounds. Various supplements of garlic, particularly aged garlic extract (AGE), are known to possess a promising antioxidant potential and are effective in prevention of chronic diseases because of the bioactive constituents. Although all of active ingredients of AGE are not elucidated, water-soluble components of AGE, including S-allylcysteine, S-allylmercaptane, steroid saponins, tetrahydro-
-carboline derivatives, and fructosyl-arginine, appears to be associated with the pharmacological effects of AGE. Consequently, the allicin free garlic components such as S-allylcysteine, S-allylmercaptane, steroid saponins, tetrahydro-
-carboline derivatives, and fructosyl-arginine can be applicable to standardization of the quality of commercial garlic products. This review provides an insight into garlic's biomarkers and presents evidence that they may either prevent or delay chronic disease associated with aging.
Characteristics of Pinitol as a Functional Food Biomaterial
Son, Min-Sik ; Seo, Myung-Seon ; Lee, Sang-Han ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 147~151
DOI : 10.5352/JLS.2009.19.1.147
D-pinitol, another chemical structure of 3-O-methyl-D-chiro-inositol, is an important insulin-sensitizer. The purpose of this review is to examine the characteristics of pinitol and other analogs as functional food biomaterials which were well known to reduce blood glucose levels. Pinitol can be converted to chiro-inositol in normal humans, while diabetic patients can not use the molecule, resulting in exhibiting low level of chiro-inositol in their urine. Recently, it is reported that pinitol can trigger phospholipase C/D, thus the rate of glucose metabolism accelerates to use as fuel for human body. To not only reduce insulin resistance of diabetic patients, but also alleviate the symptoms of diabetes, obesity, and muscle contraction, pinitol and its dietary supplementation is needed.
A Gene-based dCAPS Marker for Selecting old-gold-crimson (og
) Fruit Color Mutation in Tomato
Park, Young-Hoon ; Lee, Yong-Jae ; Kang, Jum-Soon ; Choi, Young-Whan ; Son, Beung-Gu ;
Journal of Life Science, volume 19, issue 1, 2009, Pages 152~155
DOI : 10.5352/JLS.2009.19.1.152
The old-gold-crimson (
) fruit color mutation produces deep red tomato fruit with high lycopene content. age is a null mutation allele of lycopene-
-cyclase (Crt-b) gene (B locus) that converts lycopene to
-carotene in the cartenoid biosynthesis pathway in tomato. Breeding of high lycopene tomato cultivars can be accelerated by marker-assisted selection (MAS) for introgression of
allele by using a gene-based DNA marker. In order to develop a marker, single nucleotide deletion of adenine(A) with. in a poly-A repeat that has been known to be responsible for frame-shift mutation of
was confirmed by resequencing mutant allele and wild-type allele at B locus of several tomato lines. For allele discrimination and detection of
, derived CAPS (dCAPS) approach was used by designing a primer that artificially introduced restriction enzyme recognition site of Hin fI in PCR products from
allele. This dCAPS marker is co-dominant gene-based PCR marker that can be efficiently used for MAS breeding program aiming the development of high lycopene tomato.