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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 20, Issue 12 - Dec 2010
Volume 20, Issue 11 - Nov 2010
Volume 20, Issue 10 - Oct 2010
Volume 20, Issue 9 - Sep 2010
Volume 20, Issue 8 - Aug 2010
Volume 20, Issue 7 - Jul 2010
Volume 20, Issue 6 - Jun 2010
Volume 20, Issue 5 - May 2010
Volume 20, Issue 4 - Apr 2010
Volume 20, Issue 3 - Mar 2010
Volume 20, Issue 2 - Feb 2010
Volume 20, Issue 1 - Jan 2010
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Protective Effect of White-Skinned Sweet Potato (Ipomoea batatas L.) from Indonesia on Streptozotocin-Induced Oxidative Stress in Rats
Bachri, Moch. Saiful ; Jang, Hye-Won ; Choi, Jong-Won ; Park, Jong-Ok ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1569~1576
DOI : 10.5352/JLS.2010.20.11.1569
Sweet potato (Ipomoea batatas L.) is widely used in Indonesia and other countries as a traditional medicine for the treatment of diabetes mellitus (DM). The MeOH extract of white skinned sweet potatoes (WSSP) was administered orally in doses of 100 and 200 mg/kg body weight in streptozotocin (STZ)-induced diabetic rats. Experimental diabetes was induced by a single dose of STZ (45 mg/kg, i.p.) injection. Oxidative stress was measured by tissue lipid peroxide (LPO) levels, serum aspartate transaminase (AST), alanine transaminase (ALT), total triglyceride (TG), total cholesterol (TC) and by antioxidative enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase in the liver. An increase in blood glucose, LPO level, AST, ALT, TG and TC levels was observed in the STZ-induced diabetic rats. Administration of MeOH extract of WSSP at a dose of 200 mg/kg for two weeks caused a significant reduction in blood glucose, LPO levels, AST, ALT, TG and TC levels in the STZ-induced diabetic rats. Furthermore, oral administration of MeOH extract showed significant improvement in the activities of antioxidant enzymes (SOD, GPx, and CAT) compared to STZ-induced diabetic rats. In conclusion, the obtained results clearly indicate the role of oxidative stress in the induction of diabetes, and that the protective effects of MeOH extracts of WSSP could be used to benefit diabetic patients.
Expression and Production of Human Granulocyte Colony Stimulating Factor (G-CSF) in Silkworm Cell Line
Park, Jeong-Hae ; Jang, Ho-Jung ; Kang, Seok-Woo ; Goo, Tae-Won ; Chung, Kyung-Tae ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1577~1581
DOI : 10.5352/JLS.2010.20.11.1577
Granulocyte colony stimulating factor (G-CSF) is a hematopoietic cytokine that stimulates bone marrow cells to proliferate and differentiate into granulocytes. G-CSF is approved and used for therapeutic purposes. The endoplasmic reticulum (ER) signal peptide of hG-CSF was replaced with silkworm-specific signal peptides to express and efficiently secrete recombinant hG-CSF by silkworm cells. Plasmids that contain cDNAs for hG-CSF and hG-CSF fused with silkworm- specific signal peptides of prophenoloxidase activating enzyme (PPAE), protein disulfide isomerase (PDI), and bombyxin (BX) were constructed. The G-CSF protein was expressed in insect cell line BM5 and was detected by western blot analysis. The cells transfected with plasmids containing rhG-CSF genes with silkworm-specific signal sequences released mature rhG-CSF protein more efficiently than the cells transfected with pG-CSF, the plasmid containing human G-CSF gene, including its own signal sequence. The production of hG-CSF reached maximal level at four days post-transfection and remained at a high level until 7 days post-transfection. These data demonstrate that the modification of the human G-CSF mimic to insect proteins synthesized in ER greatly improves the production of the protein.
Characterization of Recombinant PolyG-Specific Lyase from a Marine Bacterium, Streptomyces sp. M3
Kim, Hee-Sook ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1582~1588
DOI : 10.5352/JLS.2010.20.11.1582
A new alginate lyase gene of marine bacterium Streptomyces sp. M3 had been previously cloned in pColdI vector and transformed into E. coli BL21 (DE3). In this study, M3 lyase protein without signal peptide was overexpressed by induction with IPTG and purified with Ni-Sepharose affinity chromatography. The absorbance at 235 nm of the reaction mixture and TLC analysis showed that M3 alginate lyase was a polyG-specific lyase. When M3 lyase was assayed with substrate for 10 min, optimum pH and optimum temperature were pH 9 and
. For the effect of 1mM metal ion on M3 lyase activity,
ions increased the alginate degrading activity by two-fold, whereas
ions inhibited the lyase activity completely.
did not show any strong effects on alginate lyase activity.
Immobilization of β-Glucosidase from Exiguobacterium sp. DAU5 on Chitosan Bead for Improved Enzymatic Properties
Chang, Jie ; Park, In-Hye ; Lee, Yong-Seok ; Chung, Soo-Yeol ; Fang, Shu Jun ; Chandra, M. Subhosh ; Choi, Yong-Lark ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1589~1594
DOI : 10.5352/JLS.2010.20.11.1589
Glutaraldehyde was used to cross-link chitosan beads to immobilize the crude enzyme
-glucosidase from Exiguobacterium sp. DAU5. The conditions for preparing cross-linking chitosan beads and immobilization such as concentration of glutaradehyde, cross-linking time, immobilization pH and time were optimized. The chitosan beads were cross-linked with 1.5% glutaraldehyde for 1.5 hr. The immobilized
-glucosidase had an overall yield of 20% and specific activity of 5.22 U/g. The optimized pH and temperature were 9.0 and
, respectively. More than 80% of its activity at pH 7.0-10.0, 80% at
for 2 hr and 48% at
for 1 hr, were retained. However, the immobilization product showed higher pH and thermal stabilities than free enzymes. It also showed high hydrolyzing activity on soybean isoflavone glycoside linkage. These results suggest the broad application prospects of immobilization enzymes.
Protection Effects of Allylmercaptan, Metabolite of Garlic on Endothelial Cell Injury Induced by Oxidized Low Density Lipoprotein
Yang, Seung-Taek ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1595~1602
DOI : 10.5352/JLS.2010.20.11.1595
Oxidation of low density lipoprotein (LDL) has been recognized as an important role in the initiation and progression of atherosclerosis. In this study, effects of allylmercaptan, a major metabolite compound of garlic, was studied on endothelial cell injury induced by oxidized low density lipoprotein (ox-LDL). The antioxidative activity of allylmercaptan was investigated by monitoring a thiobarbituric acid substance (TBARS). Allylmercaptan inhibited LDL oxidation induced by
at concentrations of 0.1, 1 and 10 mM in a dose dependent manner. Lactate dehydrogenase (LDH) release, as an index of cell injury, and intracellular glutathione levels were determined. Pulmonary artery endothelial cells were preincubated with allylmercaptan at
for 24 hr, washed, and then exposed to 0.1 mg/ml oxidized LDL for 24 hr. Preincubation of endothelial cells with allylmercaptan significantly prevented the LDH release and depletion of GSH. Peroxides were measured directly in 24 well plates using a fluorometric assay. Allylmercaptan inhibited release of peroxides induced by ox-LDL in pulmonary artery endothelial cells. In a free system, allylmercaptan was shown to scavenge hydrogen peroxide. The data indicate that allylmercaptan can protect pulmonary artery endothelial cells from injury caused by oxidized LDL, and suggest that allylmercaptan may be useful for the prevention of atherosclerosis.
Effect of Fucus evanescens Fucoidan on Expression of Matrix Metalloproteinase-1 Promoter, mRNA, Protein and Signal Pathway
Ku, Mi-Jeong ; Jung, Ji-Won ; Lee, Myeong-Sook ; Cho, Byung-Kyu ; Lee, Soon-Rye ; Lee, Hye-Sook ; Vischuk, Olesya S. ; Zvyagintseva, Tatyana N. ; Ermakova, Svetlana P. ; Lee, Yong-Hwan ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1603~1610
DOI : 10.5352/JLS.2010.20.11.1603
Fucoidans are sulfated fucosylated polymers from the cell wall of brown algae. We assessed the effects of Fucus evanescens fucoidan on ultraviolet-B (UVB)-induced expression of matrix metalloproteinase-1 (MMP-1) protein, mRNA, and promoter, and the phosphorylation of mitogen-activated protein kinases in vitro using an immortalized human keratinocyte cell line. Pretreatment with 10 and
fucoidan significantly inhibited UVB-induced MMP-1 protein, mRNA and promoter activity, compared to UVB irradiation alone. Extracellular signal regulated kinase activation was markedly inhibited by treatment with fucoidan, though c-JUN N-terminal kinase activity and p38 activation were only marginally affected by fucoidan. F. evanescens fucoidan may be a potential therapeutic agent for the prevention and treatment of skin photoaging.
Effect of Surfactant Micelles on Oxidation in W/O/W Multiple Emulsion
Cha, Woen-Seup ; Cho, Young-Je ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1611~1616
DOI : 10.5352/JLS.2010.20.11.1611
The purpose of this research was to determine the effect of surfactant micelles on lipid oxidation in W/O/W multiple emulsions. The content of ferric irons and hydroperoxide in the continuous phase in W/O/W multiple emulsions was measured as a function of Brij micelle. The concentration of ferric iron and hydroperoxide in the continuous phase increased with increased storage time (1~6 days). Lipid oxidation rates, as determined by the formation of lipid hydroperoxides, TBARs and headspace hexanal, in the W/O/W multiple emulsions containing ferric iron decreased when 3% surfactant micelles were exceeded. These results indicate that excess surfactant micelles could alter the physical location and prooxidant activity of iron in W/O/W multiple emulsions.
Studies of Inhibitory Mechanism on Melanogenesis by Partially Purified Asiasari radix in α-MSH Stimulated B16F10 Melanoma Cells
Jang, Ji-Yeon ; Kim, Ha-Neui ; Kim, Yu-Ri ; Kim, Byung-Woo ; Choi, Yung-Hyun ; Choi, Byung-Tae ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1617~1624
DOI : 10.5352/JLS.2010.20.11.1617
Recently, it has been found that Asiasari radix showed a hypopigmenting effect on melanogenesis through activation of mitogen-activated protein kinase (MEK)/extracellular signal-activated kinase (ERK) in B16F10 melanoma cells. However, the hypopigmenting effect of A. radix on the
-melanocyte stimulating hormone (
-MSH)-stimulated melanogenesis has remained unknown. The purpose of this study was to investigate the inhibitory mechanism of the partially purified A. radix (PPAR)-induced hypopigmentating effects on
-MSH-stimulated melanogenesis in B16F10 mouse melanoma cells. PPAR strongly inhibited tyrosinase activity and leads to decreased melanin synthesis in
-MSH-stimulated B16F10 melanoma cells. PPAR also decreased the
-MSH-induced over-expression of the melanogenic enzymes, tyrosinase, tyrosinase-related protein (TRP)-1, dopachrome tautomerase (Dct) and microphthalmia-associated transcription factor (MITF). We further showed that PPAR inhibits
-MSH-induced melanogenesis via phosphorylation of MEK/ERK and PI3K/Akt, and that their activation was blocked by MEK inhibitors, PD98059 and PI3K inhibitors, LY294002 in
-MSH-stimulated B16F10 melanoma cells. These results suggest that PPAR inhibits
-MSH-induced melanogenesis by activation of MEK/ERK and PI3K/Akt through MITF degradation, which may lead to down-regulation of tyrosinase.
Chemical Components and Antioxidative Effects of Eriobotrya japonica Lindl. Leaf
Hwang, Yun-Gyeong ; Lee, Jae-Joon ; Kim, Ah-Ra ; Lee, Myung-Yul ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1625~1633
DOI : 10.5352/JLS.2010.20.11.1625
This study was carried out to investigate the physicochemical compositions and antioxidative effects of Eriobotrya japonoca Lindl (Loquat). The proximate compositions of the loquat leaf on a dry matter basis were 8.78% moisture content, 6.74% crude protein, 7.87% crude fat, 6.99% crude ash, 43.61% dietary fiber and 26.01% carbohydrate. In analysis of free amino acids, 16 kinds total amino acid components, 17 kinds of components were isolated from loquat. The essential amino acids contained in loquat leaf accounted for 50.15% of total amino acids, while the non-essential amino acids accounted for 49.85%. In analysis of total fatty acids, only 5 kinds of acid were detected: lauric acid, myristic acid, pentadecanoic acid, stearic acid and oleic acid. The contents of vitamin A, vitamin E and vitamin C were 0.039 mg%, 0.096 mg% and 0.575 mg%, respectively. The mineral contents of loquat leaf were greater in order of Zn
Induction of Physiological Sex-Reversal and Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss
Lee, Cheul-Ho ; Kim, Dae-Jung ; Jeong, Chang-Hwa ; Choi, Gyeong-Cheol ; Lee, Chae-Sung ; Kim, Dong-Soo ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1634~1639
DOI : 10.5352/JLS.2010.20.11.1634
This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 mg of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at
. More than 93% of the male population was produced by this method and growth of hormone-treated fish at
was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least
was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at
for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.
Evaluation of In-vitro Anticoagulation Activity of 35 Different Seaweed Extracts
Ahn, Seon-Mi ; Hong, Yong-Ki ; Kwon, Gi-Seok ; Sohn, Ho-Yong ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1640~1647
DOI : 10.5352/JLS.2010.20.11.1640
Seaweeds have been recognized as a health food, having anti-obesity, anti-constipation and anticoagulation activities, and the use of seaweeds in the food, medicine, and cosmetic industries have recently significantly increased. In this study, methanol extracts were prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta), and thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT) were determined in order to develop safe and novel anticoagulation agents from natural products. In TT experiments, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis (Kjellman) Setchell, Ishige foliacea, I. okamurai, Sargassum confusum and S. yamade showed strong thrombin inhibition activity among the 35 different seaweeds. In PT experiments, the inhibitions of prothrombin were identified in the selected seaweeds from TT experiment, with the exception of S. yamade. In aPTT experiments, the seaweeds with blood coagulation inhibition factors were E. cava, E. stolonifera, E. bicyclis (Kjellman) Setchell, I. foliacea, I. okamurai, S. confusum and Hixikia fusiforme Okamura. Further anticoagulation assay with the selected 8 seaweeds suggested that S. confusum is most effective in antithrombosis, and E. stolonifera, E. bicyclis (Kjellman) Setchell, and I. foliacea have high potential as antithrombosis agents. Based on components-activity correlation analysis, flavonoids are considered as active anticoagulation components of seaweeds These results suggest that edible seaweeds, especially S. confusum, have potential as safe and novel anticoagulants, and S. yamade and H. fusiforme Okamura could be used as a thrombin-specific and coagulation factor-specific inhibitors.
Flavor Components of Mideoduck (Styela clava)
Choi, Byeong-Dae ; Oh, Bong-Se ; Kang, Seok-Joong ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1648~1655
DOI : 10.5352/JLS.2010.20.11.1648
The flavor extracts of Mideoduck muscle and its juices were concentrated by simultaneous distillation and extraction (SDE) and solid-phase microextraction (SPME) methods. Each component present in the extracts was identified with GC and GC-MS by the n-paraffin hydrocarbon retention index and standard MS library data system. By SDE,
of (Z)-4,5-dimethylhex-2-en-4-ol with other alcohols, aldehydes and acids were present in the flavor extracts, with the alcohols having the highest composition and being the most important factor in Mideoduck muscle flavor. By SPME, 9 alcohols, 1 acid, 1 aldehyde, 1 hydrocarbon, 1 ester, 1 amine and 2 ketones were detected in the extracts, with alcohol such as 1-nonanol, 1-decanol and 1-tridecanol as the major components. In SPME, the muscle sample, consisting of
of (E)-2-butenedioic acid dibutylester, and
of heptadecanoic acid made up the 62.1% of total flavor extracts of Mideoduck muscle. The results of the SPME methods were similar to the composition of the raw material flavor of the sample even at a low concentration.
Hydrolysis of Egg Yolk Protein in a Packed Bed Reactor by Immobilized Enzyme
Kang, Byung-Chul ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1656~1661
DOI : 10.5352/JLS.2010.20.11.1656
Alkaline protease for the hydrolysis of egg yolk protein was immobilized on five carriers - Duolite A568, Celite R640, Dowex-1, Dowex 50W and Silica gel R60. Duolite A568 showed a maximum immobilization yield of 24.7%. Optimum pH for the free and immobilized enzyme was pH 8 and 9, respectively. However, no change was observed in optimum temperature (
). Thermal stability was observed in immobilized enzymes compared to free enzymes. The immobilized enzyme retained 86% activity after 10 cycle operations in a repeated batch process. The effect of flow rate on the stability of enzyme activity in continuous packed-bed reactor was investigated. Lowering flow rate increased the stability of the immobilized enzyme. After 96 hr of continuous operation in a packed-bed reactor, the immobilized enzyme retained 83 and 61% activity when casein and egg yolk were used as a raw materials, respectively.
Effect of Water Temperature on the Growth of Triops longicaudatus (LeConte) (Notostraca: Triopsidae)
Kwon, Soon-Jik ; Jun, Yung-Chul ; Park, Jae-Heung ; Won, Doo-Hee ; Seo, Eul-Won ; Lee, Jong-Eun ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1662~1666
DOI : 10.5352/JLS.2010.20.11.1662
Growth and other biological processes in aquatic organisms are particularly dependent on water temperatures. This study examined the effects of water temperature on the growth of Triops longicaudatus. The influence of water temperature fluctuations was that growth rate was increased at higher temperatures. The mean carapace length was 5.7 (
) mm in a water temperature of
and 7.5 (
) mm in a water temperature of
on the 14th day after submergence. It was 6.9 (
) mm in a water temperature of
and 7.8 (
) mm in a water temperature of
on the 21st day after submergence. The mean carapace length grew rapidly within 14 days after submergence, but increase in carapace length beyond this time was slow. The influence of water depth fluctuations was low as the mean carapace length was 9.3 (
) mm under a water depth of 80 mm and 9.5 (
) mm under a water depth of 190 mm on the 19th day after submergence. Biomass showed that the carapace length of 5, 10, 16 and 20 mm was a dry-weight of 1.1 (
), 18.0 (
), 26.0 (
) and 52.3 (
) mg respectively. The number of eggs increased rapidly with increments in carapace length. The mean number of eggs was 20 (
) at a carapace length of 7.0 mm, but at a carapace length of 17.0 mm, the mean number of eggs was 560 (
). The results suggested that differences in water temperature accounted for the differences in length of the carapace and the number of eggs.
Influence of Nitrate on Growth, Chlorophyll Content, Content and Activity of Rubisco and Rubisco Activase of Tobacco Plant Treated with Cadmium in vitro
Roh, Kwang-Soo ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1667~1674
DOI : 10.5352/JLS.2010.20.11.1667
Influence of nitrate on growth, chlorophyll content, content and activity of rubisco and rubisco activase of tobacco plant cultured on MS medium treated with cadmium in vitro was studied. In vitro growth and chlorophyll content reduced at 0.2 mM Cd was recovered by nitrate and this recovery was most significant at 80 mM nitrate. Rubisco content at 80 mM nitrate was more increased compared to that at other concentrations. A similar change was also shown in rubisco activity. These resultsindicate that the activation and induction of rubisco reduced by Cd were recovered by nitrate. The degree of intensity of 55 and 15 kD polypeptides identified as the large and small subunits of rubisco by SDS-PAGE analysis at 80 mM nitrate was significantly higher than that at other concentrations. The content and activity of rubisco activase at 80 mM nitrate was significantly increased than that at other concentrations. These data suggest that the recovery effects of rubisco by nitrate may be associated with rubisco activase.
Physiological Activities of Hot Water Extracts from Ecklonia cava Kjellman
Cho, Eun-Kyung ; Choi, Young-Ju ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1675~1682
DOI : 10.5352/JLS.2010.20.11.1675
The biological activity of hot water extract from Ecklonia cava Kjellman (ECE) was investigated to assess antioxidative, anti-skin aging, and nitrite scavenging abilities, as well as alcohol metabolizing activities. Antioxidant activity of ECE was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD)-like activity. DPPH radical scavenging activity and SOD-like activity of ECE increased in a remarkably dose-dependent manner, and were about 91.4% and 75% at 1 mg/ml, respectively. The xanthine oxidase inhibitory activity was indicated to be about 70% at 1 mg/ml of ECE. Nitrite scavenging ability of ECE showed to be 93.6% at 1 mg/ml and pH 1.2. The influence of ECE on alcohol metabolism was demonstrated through the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). The facilitating rate of ADH and ALDH activity by ECE was 167.2% and 334% at 10 mg/ml, respectively. In addition, tyrosinase and elastase inhibitory activities of ECE were 58% and 72% at 10 mg/ml, respectively. These results indicated that ECE has valuable biological attributes owing to its antioxidant, nitrite scavenging, alcohol metabolizing, and elastase and tyrosinase inhibitory activities.
Effects of Isoflavone Supplementation on Lipid Profiles and Antioxidant Systems in Rats Fed with Cholesterol Diet
Kim, Soo-Yeon ; Kim, Soon-Young ; Chung, Chung-Eun ; Yoon, Sun ; Park, Jung-Hwa ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1683~1690
DOI : 10.5352/JLS.2010.20.11.1683
There is an increasing interest in the potential of isoflavone in reducing the risk of cardiovascular disease and cancer, however, although several effects of isoflavone as a component of soy protein are well established, the hypolipidemic and antioxidant effects of purified isoflavone are still controversial. This study was to investigate the effects of isoflavone on serum lipid profiles and antioxidant status in rats. 7-week old male Sprague Dawley rats were fed one of the following diets for 8 weeks: basal diet (B), basal+0.3% isoflavone (BI), basal+0.5% cholesterol (BC), or basal+0.3% isoflavone +0.5% cholesterol (BIC). Two-way ANOVA was used to test the effects of dietary isoflavone and cholesterol supplementation and their interaction on variables. Serum lipid profiles and total antioxidant status (TAS) were examined spectrophotometrically. Degree of serum lipid peroxidation was measured by malondialdehyde (MDA) assay. The activities of serum antioxidant enzymes (GSH-Px, total-SOD) was determined. Levels of serum total cholesterol, VLDL+LDL-cholesterol and Atherogenic index were significantly lower in BI than those levels in group B (p=0.0002, p<0.0001, and p=0.0042, respectively). Serum total antioxidant status (TAS) levels were significantly higher, in both isoflavone supplemented groups (BI, BIC) compared to those levels in each control group (B, BC) (p<0.0001). Activity of total-SOD was significantly higher in BI compared to the activities in group B (p=0.0317). There was no interaction between isoflavone and cholesterol supplementation. In conclusion, isoflavone supplementation showed positive effects on the serum lipid profiles and total antioxidant activities in both conditions, either when fed a diet with or without cholesterol. These effects of isoflavone were independent of cholesterol supplementation.
Optimization of Production Yield for Neohesperidin by Response Surface Methodology
Yang, Hee-Jong ; Jeong, Seong-Yeop ; Choi, Nack-Shick ; Ahn, Keug-Hyun ; Park, Chan-Sun ; Yoon, Byoung-Dae ; Ryu, Yeon-Woo ; Ahn, Soon-Cheol ; Kim, Min-Soo ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1691~1696
DOI : 10.5352/JLS.2010.20.11.1691
Neohesperidin is a natural new nutrition sweetener, widely existing in plants of dry citrus peel, which can be derived from extraction. Since the sweetness is 1,300-1,500 times greater than that of sugar, neohesperidin are widely used in fruit juices, wines, beverages, bakeries and pharmaceutical formulations, and are particularly suitable for consumption by diabetic patients. However, the yield of extraction from citrus peel waste is very low. In this study optimal yield conditions were determinedusing response surface methodology (RSM) in order to increase the neohesperidin extraction yield. The critical factors for maximum extraction yield were selected extraction pressure (
), extraction time (
), and concentration of ethanol (
). As a result, the extraction yield was improved when the extracting pressure increased. The extraction yield also increased in a time-dependent manner. When adding ethanol as an assistance solvent to the supercritical carbon dioxide, extraction yield was increased as more ethanol concentration was added. Finally, the extraction yield of neohesperidin was improved to about 162.22% compared to ethanol extraction as a conventional method.
Phylogenic Study of Genus Asarum (Aristolochiaceae) in Korea by trnL-trnT Region
Lee, Byeong-Ryong ; Kim, Seon-Hoan ; Huh, Man-Kyu ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1697~1703
DOI : 10.5352/JLS.2010.20.11.1697
Asarum consists of low-growing herbs and is a genus in the Aristolochiaceae family with species found in the north temperate zones with most species in Asia. We evaluated the nine taxa with the trnL - trnT region of the chloroplast genome to estimate phenotypic relationships within genus Asarum in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Asarum was mostly due to nucleotide inserts/deletions, although several indels and inserts were found. Another source of sequence divergence was length variation due to stretches of short repeats that occur at the trnL - trnT region in all the Asarum. A + T content for nine Korean species of genus Asarum ranged between 74.7% and 78.3%. These values were higher than those for the angiosperm alignments of the total trnL and trnT region (64.5~67.1%). Within genus Asarum, A. patens was strikingly different from the others in the three phylogenetic analyses (MP, ML, and NJ). However, some internal nodes were poorly supported. Within Korean Asarum, four species were unsolved portions. Possible reasons for the striking non-congruence between the previous morphological traits and the trnL - trnT based on phylogeny were discussed.
The Scientific Research of Rehabilitation Training Program Participants in Stroke Patients
Jin, Young-Wan ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1704~1710
DOI : 10.5352/JLS.2010.20.11.1704
The purpose of this study was to describe the biomechanical characteristics of stroke patients. These characteristics were obtained during walking on a Zebris system, cinematography system and EMG system. Seven female stroke patients participated in this study. The magnitude of the profiles (joint peak angle, joint peak moments, foot pressure COP, EMG data) correlated with rehabilitation training duration using t-test. The significance level selected for this study was p<0.05, t-test. Joint analysis identified significant differences in hip joint peak angle and hip joint peak moment. Foot pressure verified significant differences in gait line length of COP. The EMG signal proved significant differences in rectus femoris and vastus lateralis.
Fibrinolytic Activity and Antioxidant Effects of the Newly Developed Agabean Fermented of Product Produced by Bacillus sp.
Kim, Sung-Ryeal ; Kim, Min-Jeong ; Lee, Hye-Hyeon ; Seo, Min-Jeong ; Kang, Byoung-Won ; Joo, Woo-Hong ; Park, Jeong-Uck ; Rhu, Eun-Ju ; Hwang, Young-Hyun ; Jeong, Yong-Kee ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1711~1717
DOI : 10.5352/JLS.2010.20.11.1711
In this study, fibrinolytic activities of fermented yellow agabean (FYA) and black agabean (FBA), and the antioxidation efficiencies of 70% ethanol extract of fermented yellow agabean (FYAE) and black agabean (FBAE) were investigated by selecting Bacillus sp. sm26 strain. Fibrinolytic activities of FYA and FBA were
, which were 1.3 and 1.4 times higher than that of FSB, respectively. With regard to total phenolic contents, FYAE and FBAE were
respectively, suggesting that their contents were about twice as high as that of fermented soybean extract (FSBE) used as a control. In comparison with FSBE, total protein and sugar contents of FYAE were
, respectively, and those of FBAE were
, respectively. This result suggests that FYAE was 4.7 and 1.7 times higher than FSBE, respectively. The DPPH radical scavenging activity of FBAE was 79% at 1 mg/ml, which was highest among the fermented bean extracts, and was twice as high as FSBE in regards to activity. In addition, FBAE exhibited the highest reducing power at 1 mg/ml, which was higher than FSBE by two-fold. With regard to lipid peroxidation, FBAE and FYAE were 93% and 80% at 1 mg/ml, which were 3 and 2.5 times higher than FSBE, respectively. Of note, the hydrogen peroxide scavenging activities of FBAE and FYAE were 82% and 54% at 1 mg/ml, offering activity that was 4 and 2.5 times higher than FSBE, respectively. Based on these results, the fibrinolytic activity and antioxidation efficiency of the fermented agabeans were significantly higher than other soybeans. Therefore, these studies may suggest that the functional agabeans can be a potential candidate for a natural functional food.
Bioactive and Chemical Properties by Silkworm (Bombyx mori L.) Powder Degradation with Kiwifruit, Papaya, Pineapple and Pear Juice
Cha, Jae-Young ; Kim, Yong-Soon ; Ahn, Hee-Young ; Eom, Kyung-Eun ; Heo, Su-Jin ; Cho, Young-Su ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1718~1724
DOI : 10.5352/JLS.2010.20.11.1718
Bioactive and chemical properties of silkworm powder (SP) degradation by fruit extract containing the proteolytic enzymes of kiwifruit, papaya, pineapple and pear were investigated. Silkworm powder was incubated with extracts from each fruit at
for 24 hr. Protein content was slightly higher in the SP treated with fruit extract than that in the control SP. Major minerals were K, Ca, Mg, and Zn. Major fatty acids were linolenic acid, oleic acid, and palmitic acid. When total protein patterns were analyzed by SDS-polyacrylamide gel electrophoresis (PAGE), silkworm protein was strongly degraded by the treatment of fruit extract from pineapple, papaya, and pear, but little silkworm degradation was observed in kiwifruit extract treatment. Fibriolytic activity was only detected in the SP by the fruit extract treatments from papaya and pear. DPPH radical scavenging activity was slightly stronger in the SP treated with fruit extract than that in silkworm powder. However, all these samples exhibiteda relatively low activity compared with the butylated hydroxytoluene (BHT). These results may provide the basic data for understanding the biological activities and chemical characteristics of SP treated with fruit extract for development of functional foods.
Normal Lung Ventilation/Perfusion Scintigraphy in Miniature Pigs
Kim, Se-Eun ; Han, Ho-Jae ; Shim, Kyung-Mi ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1725~1728
DOI : 10.5352/JLS.2010.20.11.1725
Miniature pigs are widely used in experiments related to pulmonary disease because of their similarities with humans. However, there are not enough data about normal lung function in miniature pigs. Thus, in this study, we investigated normal lung function in miniature pigs with lung ventilation/perfusion scintigraphy and evaluated the availability of this method. Three male miniature pigs weighing 30-35 kg were used. After general anesthesia, ventilation scintigraphy was performed with 100 MBq of
), after which perfusion scintigraphy was performed with intravenous injection of
-macro aggregated albumin (MAA). The functional contribution of the right lung was about 55%, and left lung was about 45%, similar to humans. Lung ventilation/perfusion scintigraphy was very useful in evaluating the normal lung function of miniature pigs because it was a non-invasive procedure (no tissue damage was involved), took a short time and was easy to perform. In conclusion, miniature pigs are similar to humans in functional contributions of the lung, and this method will be helpful in future pulmonary disease studies involving miniature pigs.
Cell Growth and Antioxidant Activity on Onion Juice Fermentation by Using Lactobacillus plantarum as Animal Probiotics
Chang, Woo-Kyung ; Cho, Sang-Buem ; Kim, Dong-Woon ; Lee, Sang-Suk ; Kim, Soo-Ki ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1729~1737
DOI : 10.5352/JLS.2010.20.11.1729
This study was conducted to establish the optimal medium condition for the animal probiotic Lactobacillus plantarum by using onion juice. Cell yield and antioxidant activity increased in proportion to high additive levels of onion juice in medium. Onion juice, sucrose and yeast extract were selected as media ingredient factors and the effects of their mixed ratio in medium were evaluated. The full factorial design consisted of 24 experimental runs and was employed to estimate the main effects of the factors and their interactions. Significant positive effects on cell yield and antioxidant activity was shown with yeast extract and onion juice, respectively. Significant interaction was found only between sucrose and yeast extract in antioxidant activity. Finally, we selected an optimal medium that was composed of (g/l) onion juice, 600; sucrose, 15; yeast extract, 5. The efficiency of this optimum medium was estimated by using a 5 l jar fermenter. As a result, the maximum cell yield was
(CFU/ml) at 12 hr. Cell yield at the end of incubation (20 hr) was
(CFU/ml) and it was very similar with the predicted value,
(CFU/ml). Antioxidant activity of culture was maintained at about 60~65% during all incubation time, resulting in a higher-than-predicted activity of 47.1%.
A New Bioluminescent Rat Prostate Cancer Cell Line: Rapid and Accurate Monitoring of Tumor Growth
Lee, Mi-Sook ; Jung, Jae-In ; Kwon, Seung-Hae ; Shim, In-Sop ; Hahm, Dae-Hyun ; Han, Jeong-Jun ; Han, Dae-Seok ; Yoonpark, Jung-Han ; Her, Song ;
Journal of Life Science, volume 20, issue 11, 2010, Pages 1738~1741
DOI : 10.5352/JLS.2010.20.11.1738
Caliper measurements of tumor volume have been widely used in the assessment of tumors in animal models. However, experiments based on caliper data have resulted in unreliable estimates of tumor growth, due to necrotic areas of tumor mass. To overcome this systematic bias, we engineered a new luciferase-expressing rat prostate cancer cell line (MLL-Luc) that produces bioluminescence from viable cancer cells. MLL-Luc cells showed a strong correlation between bioluminescence intensity and cell number (
=0.99) and also accurately quantified tumor growth, with reduced bioluminescence signals caused by necrotic cells in a subcutaneous MLL-Luc xenograft model. The accurate quantification of tumor growth with bioluminescence imaging (BLI) was confirmed by a better antitumor effect of combination chemotherapy, compared to that based on caliper measurements with a correlation between the bioluminescence signal and tumor volume (
=0.84). These data suggest that bioluminescent MLL xenografts are a powerful and quantitative tool for monitoring tumor growth and are useful in evaluating the efficacy of anticancer drugs, with less systematic bias.