Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Life Science
Journal Basic Information
Journal DOI :
Korean Society of Life Science
Editor in Chief :
Volume & Issues
Volume 20, Issue 12 - Dec 2010
Volume 20, Issue 11 - Nov 2010
Volume 20, Issue 10 - Oct 2010
Volume 20, Issue 9 - Sep 2010
Volume 20, Issue 8 - Aug 2010
Volume 20, Issue 7 - Jul 2010
Volume 20, Issue 6 - Jun 2010
Volume 20, Issue 5 - May 2010
Volume 20, Issue 4 - Apr 2010
Volume 20, Issue 3 - Mar 2010
Volume 20, Issue 2 - Feb 2010
Volume 20, Issue 1 - Jan 2010
Selecting the target year
Differential Expression Patterns of Gangliosides in the Liver and Heart of NIH-miniature Pigs
Ryu, Jae-Sung ; Chang, Kyu-Tae ; Kim, Ji-Su ; Kwak, Dong-Hoon ; Lee, Young-Choon ; Oh, Keon-Bong ; Choo, Young-Kug ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 467~473
DOI : 10.5352/JLS.2010.20.4.467
Gangliosides are a major component of the plasma membrane of mammalian cells, which are directly involved in a variety of immunological events, including cell-to cell or cell-to-protein interactions. In this study, we investigated whether gangliosides, sialic acid-containing glycosphingolipids, are related to rejection during the xenotransplantation of NIH-miniature pig livers and hearts to humans. Both high performance thin-layer chromatography and immunohistochemistry analyses revealed that the expression of gangliosides in the liver tissue of NIH-miniature pigs was higher than that in the heart. Gangliosides GD3, GD1a, GD1b, GT1b and GQ1b were observed in both the liver and heart, whereas GQ1b was detected only in the liver, indicating that the ganglioside expression profiles are tissue specific. Moreover, other ganglio-series gangliosides, including GM3, were not detected in the livers and hearts of NIH-miniature pigs. Taken together, these results suggest that gangliosides may play important roles in immune responses in clinical xenotransplants of pig livers and hearts.
Rutin Ameliorates Neutrophilic Oxidative Stress-Induced Acute Lung Injury by Intratracheal IL-1 Insufflation in Rats
Kwon, Sung-Chul ; Park, Yoon-Yub ; Lee, Young-Man ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 474~480
DOI : 10.5352/JLS.2010.20.4.474
Rutin, a group II phospholipase
) inhibitor, was tested on interleukin-1 (IL-1) induced acute lung injury (ALI) in male Sprague-Dawley rats. Rutin did not alter the increased lung myeloperoxidase activity by IL-1. However, the number of neutrophils in bronchoalveolar lavage fluid (BALF) and IL-1 induced lung leak were decreased by rutin (p<0.001). Simultaneously, rutin decreased lung
activity, which was increased by IL-1 (p<0.001). The reduction of neutrophilic respiratory burst by the inhibition of
was confirmed by group II
inhibitors such as rutin, manoalide and scalaradial. The increased level of cytokine-induced neutrophilic chemoattractant (CINC) in BALF by IL-1 was not affected by rutin. Ultrastructural changes of ALI and increased generation of free radicals in the lung by IL-1 were found, and rutin ameliorated these pathological findings. Taken together, rutin seems to be effective in decreasing IL-1 induced ALI through inhibition of group II
The Rates of Synonymous and Nonsynonymous Substitutions in Sorbus aucuparia Using Nuclear and Chloroplast Genes
Huh, Man-Kyu ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 481~486
DOI : 10.5352/JLS.2010.20.4.481
The rates of synonymous and nonsynonymous nucleotide substitutions were studied for sequences of nuclear and chloroplast genes in Sorbus aucuparia. Results suggested that DNA evolution in this species had taken place, on average, at a slower rate in the chloroplast genes than in the nuclear genes: a rate variation pattern similar to those observed in eudicot plants. Within the nucleus, the synonymous substitution rates (Ks) (2.45-2.60) were two-fold higher than nonsynonymous substitution rates (Ka) (1.15-1.30). More notably, the values of Ks (1.20-1.26) were about six-fold higher than those of Ka (0.26-0.42) within the chloroplast genome. Ka/Ks ratios for nuclear and chloroplast genes of S. aucuparia had mean values of 0.178 and 0.056, respectively. A Ka/Ks ratio < 1 indicated negative (purifying) selection. The chloroplast genes had a lower effective number of codons (ENC) values (22.4-32.2) than those of nuclear genes (35.8-38.7). The analysis of the G+C content indicated that the chloroplast genes in this investigation had a higher preference for synonymous codons ending with A and T (G+C content range, 28.4-29.1%) where there was a slight bias toward codons ending with G+C (63.2-64.2%) in the nuclear genome.
Characterization of Acidic Carboxymethylcellulase Produced by a Marine Microorganism, Psychrobacter aquimaris LBH-10
Kim, Hye-Jin ; Gao, Wa ; Lee, You-Jung ; Chung, Chung-Han ; Lee, Jin-Woo ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 487~495
DOI : 10.5352/JLS.2010.20.4.487
A microorganism hydrolyzing carboxymethylcellulose (CMC) was isolated from seawater, identified as Psychrobacter aquimaris by analysis of 16S rDNA sequences, and named P. aquimari LBH-10. This strain produced an acidic carboxymethylcellulase (CMCase), which hydrolyzed carboxymethylcellulose (CMC), cellobiose, curdlan, filter paper, p-nitrophenyl-
-D-glucopyranoside (pNPG), pullulan, and xylan, but there was no detectable activity on avicel and cellulose. The optimal temperature for CMCase produced by P. aquimari LBH-10 was
and more than 90% of its original activity was maintained at broad temperatures ranging from 20 to
after 24 hr. The optimal pH of the CMCase was 3.5, and more than 70% of its original activity was maintained under acidic conditions between pH 2.5 and 7.0 at
after 24 hr. The optimal pH of CMCase produced by P. aquimaris LBH-10 seems to be lower than those produced by any other bacterial and fungal strain.
, EDTA, and
at a concentration of 0.1 M enhanced CMCase-produced P. aquimaris LBH-10, whereas
Analgesic Effect of Grape Seed Proanthocyanidin Extract in Fibromyalgia Animal Model
Mun, Hyun-Il ; Kim, Seong-Ho ; Jang, Tae-Jung ; Moon, Il-Soo ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 496~502
DOI : 10.5352/JLS.2010.20.4.496
The acidic saline animal model of pain has been suggested to mimic fibromyalgia (FM). Oligomeric proanthocyanidin complexes (OPC) from grape seeds are known to act as an antioxidant. We studied the effects of OPC on the pain threshold in the acidic saline animal model of pain. The left gastrocnemius muscle was injected with
of saline at pH 4.0 under brief isoflurane anesthesia on days 0 and 5. Control rats (n=5) received identical injections of physiological saline (pH 7.2) on the same schedule. Rats (n=10) with acidic saline injection were separated into two study subgroups. After measurement of pre-drug pain thresholds, rats were injected intraperitoneally with either saline or OPC 300 mg/kg. Paw withdrawal thresholds to pressure were again measured 60 min after intraperitoneal injection. Nociceptive thresholds were measured with a Dynamic Plantar Aesthesiometer by applying an increasing pressure to right or left hind paw until the rat withdrew the paw. Compared to baseline (day 0), acid injections produced mechanical hyper-responsiveness on day 7 (pre-drug) in these rats [p<0.05]. A potent antihyperalgesic effect was observed when rats were injected intraperitoneally with OPC 300 mg/kg [injected paw, p=0.001; contralateral paw, p=0.002]. OPC treatment decreased the expression of acid sensing ion channel 3 in the brain motor cortex area on immunohistochemical staining when OPC 300 mg/kg was administered intraperitoneally in the animal model of FM pain [p<0.05]. Further research is required to determine the efficacy of OPC treatments in FM pain in humans.
Role of Protease Activated Receptor 2 (PAR2) in Aspergillus Protease Allergen Induces Th2 Related Airway Inflammatory Response
Yu, Hak-Sun ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 503~510
DOI : 10.5352/JLS.2010.20.4.503
Most allergens have protease activities, suggesting that proteases may be a key link between Th2-type immune reactions in allergic responses. Protease activated receptor (PAR) 2 is activated via the proteolytic cleavage of its N-terminal domain by proteinases. To know the role of PAR2 in Aspergillus protease allergen activated Th2 immune responses in airway epithelial cells, we investigated and compared immune cell recruitment and level of chemokines and cytokines between PAR2 knock out (KO) mice and wild type (WT) mice. There were evident immune cell infiltrations into the bronchial alveolar lavage fluid (BALF) of WT mice, but the infiltrations in PAR2 KO mice were significantly lowered than those of WT mice. The IL-25, TSLP, and eotaxin gene expressions were profoundly increased after Aspergillus protease, but their expression was significantly lowered in PAR2 KO mice in this study. Compared to PAR2 KO mice, OVA specific IgE concentrations in serum of WT mice were quite increased; moreover, the IgE level of PAR2 KO mice was lower than in WT mice. The IL-25 expression by Aspergillus protease stimulation was significantly reduced by p38 specific inhibitor treatment. In this study, we determined that Th2 response was initiated with IL-25 and TSLP mRNA up-regulation in lung epithelial cells via PAR2 after Aspergillus protease allergen treatment.
Studies of Exercise-Induced Allergy Anaphylaxis Mechanisms and the Effects of Vitamin C and Catalase Supplementation in Exercise-Induced Allergy Anaphylaxis Models
Kwak, Yi-Sub ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 511~518
DOI : 10.5352/JLS.2010.20.4.511
Exercise-induced anaphylaxis (EIA) is defined as the onset of allergic symptoms during, or immediately after, exercise, the clinical signs being various degrees of urticaria, angioedema, respiratory and gastrointestinal signs, and even anaphylactic shock. Food-dependent exercise-induced anaphylaxis (FDEIA) is a specific variant of exercise-induced anaphylaxis that requires both vigorous physical activity and the ingestion of specific foods within the preceding several hours. To describe the physiopathologic mechanism, etiologic factors, and clinical manifestations, we evaluated the supplementation of vitamin C and catalase on spleen index, proliferation assay, ROS, and ASAS in sensitized and exercise trained mice. The results were as follows: Spleen index showed the highest level in the ST12 group compared to other groups; this level increased in a time dependent manner and in significant amounts. In proliferation assay of Med and OVA, the ST12 group showed the highest level compared to other groups; this level also increased in a time dependent manner. On the other hand, spleen ROS did not show a statistically significant difference, and peritoneal ROS showed the highest level in the ST12 group. ASAS showed the highest level in the ST12 compared to the S; this was also in a time dependent manner and in significant amounts. From the results, we chose the ST9 and ST12 groups to evaluate allergy anaphylaxis with supplementation of Vitamin C and catalase. In both the ST9 and ST12 groups, peritoneal ROS and ASAS were lower in vitamin C treatment group than in the catalase treatment group. This was a statistically significant difference. From the results, allergy anaphylaxis showed a higher level in the long trained group than in the short trained group. Also, treatment with vitamin C was more effective in lowering allergy anaphylaxis than catalase treatment.
Paclitaxel Induced Caspase-Independent Mitotic Catastrophe in Rabbit Articular Chondrocyte
Im, Jeong-Hee ; Kim, Song-Ja ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 519~527
DOI : 10.5352/JLS.2010.20.4.519
Paclitaxel is known as a potent inhibitor of microtubule depolymerization. It leads to mitotic arrest and cell death by stabilizing the spindle in various cell types. Here, we investigated the effects of paclitaxel on the proliferation and cell death of rabbit articular chondrocytes. Paclitaxel inhibited proliferation in a dose- and time- dependent manner, determined by MTT assay in rabbit articular chondrocytes. We also established paclitaxel-induced G2/M arrest by fluorescent activated cell sorter (FACS) analysis. Paclitaxel increased expression of cyclin B, p53 and p21, while reducing expression of cdc2 and cdc25C in chondrocytes, as detected by Western blot analysis. Interestingly, paclitaxel showed the mitotic catastrophe that leads to abnormal nucleus division and cell death without DNA fragmentation through activation of caspase. Cell death by mitotic catastrophe in cells treated with paclitaxel was suppressed by inhibiting G1/S arrest with 2 mM thymidine. These results demonstrate that paclitaxel induces cell death via mitotic catastrophe without activation of casepase in rabbit articular chondrocytes.
Effects of Aged Black Garlic Extracts on the Tight Junction Permeability and Cell Invasion in Human Gastric Cancer Cells
Shin, Dong-Yeok ; Yoon, Moo-Kyoung ; Choi, Young-Whan ; Gweon, Oh-Cheon ; Kim, Jung-In ; Choi, Tae-Hyun ; Choi, Yung-Hyun ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 528~534
DOI : 10.5352/JLS.2010.20.4.528
Garlic (Allium sativum) has been well-known as a folk remedy for a variety of ailments since ancient times, and it is well documented that enhanced garlic consumption leads to a decrease in incidences of cancer. Tight junctions (TJs) are critical structures for the maintenance of cellular polarity, acting as paracellular permeability barriers and playing an essential role in regulating the diffusion of fluid, electrolytes and macromolecules through the paracellular pathway. Matrix metalloproteinases (MMPs) have been implicated as possible mediators of invasiveness and metastasis in some cancers. In this study, we investigated the potential effects of water extract of aged black garlic (ABG) on the correlation between tightening of TJs and anti-invasive activity in human gastric carcinoma AGS cells. The inhibitory effects of ABG on cell motility and invasiveness were found to be associated with increased tightness of TJs, which was demonstrated by an increase in transepithelial electrical resistance. Additionally, the activities of MMP-2 and -9 in AGS cells were inhibited by treatment with ABG, and this was also correlated with a decrease in the expression of their mRNA and proteins. Furthermore, RT-PCR and immunoblotting results indicated that ABG repressed the levels of the claudin proteins, major components of TJs that play a key role in the control and selectivity of paracellular transport. In conclusion, these results suggest that ABG treatment may inhibit tumor metastasis and invasion, and therefore may act as a dietary source to decrease the risk of developing cancer.
Anthropometric Measurements of the Upper and Lower Body Balance and Exercise Habit among Female College Students in Some Parts of the Gyeongnam
Kim, Young-Sik ; Yoon, Im-Sil ; Lee, Won-Joon ; Nam, Jeong-Su ; Yoon, Joong-Soo ; Youn, Ryea-Min ; Jung, Han-Na ; Ko, Jae-Shik ; Choi, Hyun-Ju ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 535~542
DOI : 10.5352/JLS.2010.20.4.535
This study was carried out to examine anthropometric parameters and related habits in female college students. Mean age, height, and body weight of the subjects in total (n=212) were 20.7 yrs, 161.8 cm, and 53.5 kg, respectively. Anthropometric parameters were measured by bioelectrical impedance analysis. Mean degree of obesity, body mass index (BMI), and waist-hip ratio (WHR) were 98.4%,
, and 0.80 respectively. Surprisingly, 63.7% of the subjects showed unbalanced weak upper bodies compared to their lower bodies. Therefore, they were divided into two groups: unbalanced weak upper body group (UU, n=135) and balanced upper body groups (BU, n=77). Bodyweight, degree of obesity, BMI, and WHR in the UU group were significantly lower than those in the BU group. Amounts of body protein, body fat, and body mineral in the UU group were also significantly lower than those of the BU group. Mid-arm muscle circumference of the UU group (
) was significantly lower than those of BU group (
), although mid-arm circumference of the UU group was not significantly different compared to those of the BU group. On a questionnaire on exercise habits, 1.5% of the UU group and 7.8% of the BU group answered that they exercised regularly, and 55.5% of the UU group and 31.2% of the BU group reported that they never exercised (p<0.01). Even in the subjects who exercised, the duration of the exercise was not sufficient because 38.4% of the UU group and 35.8% of the BU group answered that they exercised for less than 30 min. In conclusion, interventions in exercise habits may be needed for female college students who have unbalanced weak upper bodies.
Lower Limbs Muscle Comparative Research for Verification Effect of Rehabilitation Training Program of Total Hip Arthroplasty
Jin, Young-Wan ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 543~548
DOI : 10.5352/JLS.2010.20.4.543
The purpose of this study was to examine the differences in kinetics between 6 months of rehabilitation training and 12 months of rehabilitation training after total hip arthroplasty. 10 unilateral THA participants performed kinetic tests. Three dimensional kinematics and hip flexors and abductors electromyography (EMG) were collected during each trial. T-test was used for statistical analysis (p<0.05). There was no significant difference in EMG data between the two groups, but the mean comparison EMG data was higher in the 12 months rehabilitation training group than the 6 months rehabilitation training group. The moment value was found with motion-dependent interaction analyzing method which was used by Feltner and Dapena. There was no significant difference between moment values of the two groups. There was no significant difference between ground reaction forces of the two groups; however, there were some differences shown in Fz (vertical reaction force) between the two groups (
). The first peak impact force was about 9% lower in the 12 months group compared to the 6 months group. The second peak active force was nearly equal between the two groups. More research is necessary to determine exactly what constitutes optimal rehabilitation training biomechanics for patients with total hip arthroplasty.
Effects of Shoulder Muscle Strength on Terminal Range by Humeral Head Retroversion
Park, Si-Young ; Lee, Dong-Jun ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 549~554
DOI : 10.5352/JLS.2010.20.4.549
Increased external rotation and decreased internal rotation have been noted to occur progressively in the throwing shoulders of baseball pitchers. The purpose of this study was to provide descriptive data for terminal range eccentric antagonist/concentric agonist shoulder muscle strength in collegiate baseball pitchers with humeral head retroversion diagnosed through MRI. The dominant and non-dominant shoulders of 9 asymptomatic baseball pitchers were tested through a range of 20 degrees of external rotation to 90 degrees of internal rotation using the Biodex system 3 isokinetic dynamometer at speeds of
. Differences between the dominant and non-dominant shoulders were assessed using the paired samples t-test. Total range of motion, measured at
of glenohumeral abduction, was
for dominant shoulders and
for non-dominant shoulders. Humeral head retroversion measured
in dominant and
in non-dominant extremities. The mean internal rotator concentric contraction (IR-Con) showed a significant difference compared to
(Nm) in dominant and
(Nm) in non-dominant shoulders at
(p<0.05). The mean external rotator eccentric contraction (ER-Ecc) showed a significant difference compared to
(Nm) in dominant and
(Nm) in non-dominant shoulders at
(p<0.05). There is a pattern of increased external rotation and decreased internal rotation in the dominant extremity that significantly correlates with an increase in humeral retroversion.
Pre-Exercise Protective Effects Against Renal Ischemic Reperfusion Injury in Hsp 70.1 Knockout Mice
Lee, Jin ; Kim, Won-Kyu ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 555~560
DOI : 10.5352/JLS.2010.20.4.555
The objective of this study was to investigate levels of serum creatinine, CuSOD and MnSOD protein expression in the kidney after renal ischemic reperfusion with pre-exercise using heat shock protein 70.1 in knock-out mice (KO). The C57/BL6 strain (Wild type: WT) and KO were divided into 4 groups as follows: Sham control group (Sham), pre-exercise group (Ex), pre-exercise +ischemia group (Ex+IR), and ischemia group (IR). CuSOD and MnSOD expression were significantly decreased (p<0.01, p<0.05) and blood creatinine concentration was significantly increased (p<0.01) in the IR group of KO. In contrast, CuSOD and MnSOD expression in the Ex+IR group of KO were higher than the IR group, while creatinine concentration was significantly lower. These results suggest that Hsp70 is directly correlated to renal ischemic reperfusion injury. Pre-exercise in renal ischemia might prevent or inhibit positive oxidative stress inhibitory effects by increasing anti-oxidative enzymes (CuSOD, MnSOD) within the kidney and improve to prevent renal function. Thus, pre-exercise may have a protective role against renal injury after renal ischemia.
Mechanism of Anti-Invasive Action of Docosahexaenoic Acid in SW480 Human Colon Cancer Cell
Shin, So-Yeon ; Kim, Yong-Jo ; Song, Kyoung-Sub ; Jing, Kaipeng ; Kim, Na-Yeong ; Jeong, So-Yeon ; Park, Ji-Hoon ; Seo, Kang-Sik ; Heo, Jun-Young ; Kwon, Hyun-Joo ; Park, Jong-Il ; Park, Seung-Kiel ; Kweon, Gi-Ryang ; Yoon, Wan-Hee ; Hwang, Byung-Doo ; Lim, Kyu ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 561~571
DOI : 10.5352/JLS.2010.20.4.561
Colon cancer is one of the most common malignancies in the western world and the second leading cause of cancer death in Korea. Epidemiology studies have shown a reduced incidence of colon cancer among populations consuming a large quantity of
-polyunsaturated fatty acids (
-PUFA) of marine origin. Recently, it has been found that
-PUFA has an antineoplastic effect in several cancers. This study was designed to investigate the mechanism of the anti-invasive effect of
-PUFA in colon cancer.
-PUFA, docosahexaenoic acids (DHA) and eicosapentaenoic acid (EPA) treatment resulted in a dose-dependent inhibition of cell growth in SW480 human colon cancer cells. In contrast, arachidonic acid (AA), a
-PUFA, exhibited no significant effect. This action likely involves apoptosis, given that DHA treatment increased apoptotic cells in TUNEL assay. Moreover, invasiveness of SW480 cells was inhibited following treatment of DHA in a dose-dependent manner; in contrast, AA had no effect. The levels of MMP-9 and MMP-2 mRNA decreased after DHA pretreatment. MMP-9 and MMP-2 promoter activities were also inhibited by DHA treatment. The levels of NF-kB and p-IkB protein were down-regulated by DHA pretreatment in a dose dependent manner. In addition, DHA inhibited NF-kB promoter reporter activities. These findings suggest that
-PUFA may inhibit cancer cell invasion by inhibition of MMPs via reduction of NF-kB in colon cancer. In conclusion,
-PUFA could be used for chemoprevention and treatment of human colon cancer.
Effects of Exercise Intensity on Bcl-2, Bax, Caspase-3 Protein Level and DNA Fragmentation in Soleus and EDL Muscle on 60 wk SD Rats
Lee, Ji-Yung ; Kim, Yong-An ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 572~577
DOI : 10.5352/JLS.2010.20.4.572
The purpose of this study was to find out the Bcl-2 (B-cell leukemia/lymphoma-2), Bax, and caspase-3(cysteine-aspartic proteases-3) protein expression in soleus and EDL muscle according to treadmill exercise intensity in 60 week-old SD rats. The SD rats were randomly divided into four groups (n=10 in each group): control (CON), low intensity exercise (LE), moderate intensity exercise (ME), and high intensity exercise (HE). The exercise was given to the rats for 8 wk, 5 day/wk. The animals underwent treadmill exercise at intensities of 30 min at 8 m/min for the LE group, 15 min at 16 m/min for the ME group, and 9 min at 24 m/min for the HE group. The results were as follows: the expression of Bcl-2 protein was lowest in the HE group and the expression of Bax protein was highest in the HE group. The expression of caspase-3 (cleaved form) protein was observed in the HE group. For the different types of muscle fiber, Bcl-2 protein expression in the soleus muscle was decreased in all groups. Bax protein expression in the soleus muscle was increased in the HE group only. Bcl-2 protein expression in the EDL muscle was decreased in the HE group, and Bax protein expression in the EDL muscle was increased in the ME and HE groups. Consequently, the protein expression related to the aged rats shows a difference according to the intensity of exercise. In addition, caspase-3 protein expression appeared in the HE group; however, in all amounts of intensity, DNA fragmentation was not observed. Therefore, apoptosis on skeletal muscles of aged mice can be intervened with optimal exercise. On the other hand, high intensity exercise can potentially accelerate the apoptosis of muscle fiber in aged rats.
Identification of a Carduus spp. Showing Anti-Mycobacterial Activity by DNA Sequence Analysis of Its ITS1, 5.8S rRNA and ITS2
Bae, Young-Min ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 578~583
DOI : 10.5352/JLS.2010.20.4.578
It has been reported that extracts of globe thistle (Echinops spp.) and thistle (Circium spp., Carduus spp. and Onopordum spp.) have anti-bacterial and anti-fungal activities. Methanol extracts of Echinops setifer and Carduus spp. were used to test and see if the extracts of these plants could suppress growth of Mycobacterium smegmatis and Mycobacterium fortuitum. Although extract of Echinops setifer showed no anti-mycobacterial activities, extract of Carduus spp. showed inhibition zones when tested with filter discs. Genomic DNA was isolated from Carduus spp. and PCR was performed to clone a DNA fragment containing ITS1, 5.8S rRNA gene and ITS2. A 733-bp PCR product was obtained and its DNA sequence was reported to the GenBank (accession number GU188570). BLAST search of the obtained DNA sequence did not show a match with any DNA sequences in the Genbank. Carduus crispus and Carduus defloratus had the closest phylogenetic relationships to this plant.
Association of Clusterin Polymorphisms (-4453T<G, 5608T<C) with Coronary Heart Disease in Korean Population
Kim, Su-Won ; Yoo, Min ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 584~588
DOI : 10.5352/JLS.2010.20.4.584
Clusterin is an 80 kDa heterodimetric glycosylated protein which plays diverse biological roles in various tissues and organs. Clusterin is reported to be associated with the pathogenesis of coronary artery disease and atherosclerosis. Therefore, we investigated the genotype for the T
Verification of Antimicrobial Activities of Various Pine Needle Extracts against Antibiotic Resistant Strains of Staphylococcus aureus
Kim, Nam-Young ; Jang, Min-Kyung ; Jeon, Myung-Je ; Lee, Dong-Geun ; Jang, Hye-Ji ; Lee, Seung-Woo ; Kim, Mi-Hyang ; Kim, Sung-Gu ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 589~596
DOI : 10.5352/JLS.2010.20.4.589
We investigated antimicrobial activities of various pine (Pinus densiflora) needle extracts against antibiotic resistant strains of Staphylococcus aureus. Hot water extract showed the highest antimicrobial activity against normal and methicillin resistant Staphylococcus aureus (MRSA), however, it exhibited no antimicrobial activity against penicillin resistant S. aureus (PRSA). Hot water-hexane (HWH), hot water-ethanol (HWE), hexane, and ethanol extracts showed antimicrobial activity against S. aureus, PRSA and MRSA. Minimum inhibitory concentrations (MIC) of HWH, HWE, hexane, and ethanol extracts were 0.05, 0.05, 0.5 and 5 mg/ml, respectively, and HWH and HWE extracts showed the strongest antimicrobial activity among these extracts. Antimicrobial activities of pine needle extracts were stable after heating at
for 20 min. These results suggested that pine needle extracts can be used as an effective natural antimicrobial agent for food and medical industries.
The Growth Response of Quercus dentata Sapling to the Environmental Gradients Treatment
Lee, Sang-Kyoung ; You, Young-Han ; Yi, Hoon-Bok ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 597~601
DOI : 10.5352/JLS.2010.20.4.597
Quercus dentata (Thunb. ex Murray) is a major tree found in dry habitats such as limestone areas of Korea. In order to characterize the ecological traits of Q. dentata, we treated Q. dentata saplings under four gradient levels of major environment factors such as light, soil moisture and nutrients for 5 months in a glass house. We then measured and analyzed growth differences among them. Regarding light, aboveground, belowground and plant biomass were highest at a high gradient and lowest at a low one. The root/shoot ratio was highest at the highest light gradient. Regarding moisture, no measured items were significantly affected by the moisture gradient. Regarding nutrients, aboveground, belowground and plant biomass were the highest at a slightly high gradient and the lowest at a gradient lower or higher than this. The root/shoot ratio was not significantly affected by the nutrient gradient. From these results, it was shown that the growth of Q. dentata was more affected by light and nutrients in the environment than moisture.
Matrix Metalloproteinase-3 Gene Polymorphisms (A(-267)G, A658G, T813C) is Associated with Type 2 Diabetes in Koreans
Yoo, Min ; Kim, Hyo-Jeong ; Qing, Ye ; Kim, Jong-Won ; Kim, Su-Won ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 602~606
DOI : 10.5352/JLS.2010.20.4.602
Type 2 diabetes is a typical polygenic disease complex, for which several common risk alleles have been identified. Proteins in the matrix metalloproteinase-3 (MMP3) family are involved in the breakdown of the extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes. Therefore, we investigated the genotype for the A(-267)G, A658G and T813C polymorphisms in the MMP3 gene in the Korean population and compared genotypes of patients with those of the control group. 200 patients (male 108, female 92), who had previously been diagnosed with type 2 diabetes (T2DM) and 100 control subjects (male 36, female 64) participated in this study. There was a strong association between A(-267)G and A658G polymorphism in the MMP3 gene and T2DM. The present study shows that MMP3 polymorphisms (A(-267)G and A658G) may be associated with the pathogenesis of T2DM. Further studies with a larger population may be needed for the development of diagnostic methods at a genetic level, such as DNA chip.
Effect of Pine (Pinus densiflora) Needle Extracts on Synthesis of Collagen in Osteoblastic MC3T3-E1 Cells
Jeon, Min-Hee ; Kim, Young-Kyoung ; Park, Yong-Soo ; Hwang, Hyun-Jung ; Kim, Sung-Gu ; Lee, Sang-Hyeon ; Choi, In-Soon ; Kim, Mi-Hyang ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 607~613
DOI : 10.5352/JLS.2010.20.4.607
Osteoporosis is a disease involving a decrease in bone mineral density and an increased risk of fractures. The MC3T3-E1 pre-osteoblastic cell line is a well-accepted model of osteogenesis in vitro. Pine needles have long been used as a traditional health-promoting medicinal food in Korea. In this study, MTT assay, the alkaline phosphatase (ALP) activity and collagen synthesis of osteoblast cells were investigated to determine the effects of pine needle extracts on cell proliferation and differentiation. Pine needle extracts were prepared using hexane, ethanol and water. The effects of the pine needle extracts were examined by comparing the results with those of commercial agents, such as proanthocyanidin. The MC3T3-E1 cells exposed to proanthocyanidin showed increased proliferation in a concentration-dependent manner. The cells exposed to the hexane extract showed a similar increase in proliferation to that observed with proanthocyanidin. The hexane extract showed the highest ALP activity. Moreover, a supplement of pine needle extracts induced collagen synthesis in MC3T3-E1 cells. The pine needle extract produced the highest level of collagen synthesis at concentrations of
. These results indicate that pine needle extracts have an anabolic effect on bone by promoting osteoblastic differentiation, and may be used in the treatment of common metabolic bone diseases.
Genetic Differences between Wild and Cultured Populations in Olive Flounder in Korea Based on Mitochondrial DNA Analysis
Kim, Mi-Jung ; Kim, Kyung-Kil ; Park, Jung-Youn ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 614~617
DOI : 10.5352/JLS.2010.20.4.614
We sequenced a 522 bp fragment including the
gene and the first half of the control region from 29 wild and cultured olive flounder specimens from Korea. Out of 522 nucleotide sites, 49 (9.4%) were variable, 23 haplotypes being found. Most haplotypes are unique in the wild population and only four were shared by cultured specimins. The nucleotide diversity and differences between wild and cultured populations were
, respectively. Haplotype diversity was
in the wild and cultured populations, respectively. These results show that marked reductions of genetic variability in the hatchery strains were observed in the number of mitochondrial DNA haplotypes and haplotype diversity when compared to the wild populations. Furthermore, we detected significant population differentiation between both populations. The mtDNA sequencing technique used to evaluate the genetic variability of hatchery strains compared to that of the wild population is potential for genetic monitoring of olive flounder hatchery stocks.
The Relationship between the Urinary Arsenic and 8-Hydroxydeoxyguanosine Levels in Women of Abandoned Mine Area
Choi, Young-Sook ; Eom, Sang-Yong ; Choi, Byoung-Sun ; Park, Jung-Duk ; Kim, Yong-Dae ; Kim, Heon ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 618~622
DOI : 10.5352/JLS.2010.20.4.618
This study examines the relationship between urinary arsenic concentration and urinary 8-hydroxydeoxyguanosine (8-OHdG) concentrations, an index of oxidative DNA damage, among women who live near abandoned metal mines. The sample consisted of 165 residents living near two abandoned metal mines located in Chungbuk Province. Demographic characteristics as well as environmental factors relevant to arsenic exposure were collected through interviews, and urinary arsenic concentrations and urinary 8-OHdG concentrations of the research subjects were measured. The collected data were subsequently analyzed using the statistics program SPSS 12.0. The geometric average of urinary arsenic concentrations among the research subjects was
creatinine. In a correlation analysis between urinary arsenic and 8-OHdG concentrations, the correlation coefficient was significant (p<0.001) at 0.399. This study suggests that urinary 8-OHdG concentrations may be a DNA damage marker for chronic arsenic exposure in women.
Immuno-stimulating and Antitumor Effects on Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Hericium erinaceus
Choi, Yon-Il ; Lee, Jae-Seong ; Lee, U-Youn ; Lee, Tae-Soo ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 623~631
DOI : 10.5352/JLS.2010.20.4.623
Hericium erinaceus, an edible and medicinal mushroom belonging to the Basidiomycota family, has been used for curing gastric ulcers and stomach cancers in human beings and is also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma in mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to as Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from the fruiting body of the mushroom. In in vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at concentrations of
. Intraperitoneal injection with crude polysaccharides exhibited a life prolongation effect of 29.1~54.1% in mice previously inoculated with Sarcoma 180. Fr. Na increased the numbers of spleen cells by 2.9 fold at a concentration of
compared with the control. Fr. Na improved the immuno-potentiating activity of B lymphocytes by increasing alkaline phosphatase activity by 5.5 fold compared with the control at a concentration of
. Fr. NaCl increased the numbers of peritoneal exudate cells and circulating leukocytes by 4 and 2.3 folds at a concentration of 50 mg/kg, respectively. Therefore, the crude polysaccharides extracted from the fruiting body of H. erinaceus could improve antitumor activities in mice.
Evaluation of Genetic Diversity among Persimmon Cultivars (Diospyros kaki Thunb.) Using Microsatellite Markers
Hwang, Ji-Hyeon ; Park, Yu-Ok ; Kim, Sung-Churl ; Lee, Yong-Jae ; Kang, Jum-Soon ; Choi, Young-Whan ; Son, Beung-Gu ; Park, Young-Hoon ;
Journal of Life Science, volume 20, issue 4, 2010, Pages 632~638
DOI : 10.5352/JLS.2010.20.4.632
The genetic diversity among 48 persimmon (Diospyros kaki Thunb.) accessions, indigenous in Korea and introduced from Japan and China, was evaluated by using simple sequence repeat (SSR) markers. From 20 SSR primer sets, a total of 114 polymorphic markers were detected among 12 pollination-constant non-astringent (PCNA), 13 pollination-variant non-astringent (PVNA), 15 pollination-variant astringent (PVA), and 8 pollination-constant astringent (PCA) cultivars. Analysis of pair-wise genetic similarity coefficient (Nei-Li) and unweighted pair-group method with arithmetic averaging (UPGMA) clustering revealed two main clusters and four subclusters for cluster I. The subclustering pattern was in accordance with the classification of persimmon cultivars based on the nature of astringency loss. Phenetic relationships among the subclusters showed a closer relatedness of the PCNA group with the PVNA group, and the PVA with the PCA group. Genetic similarity co-efficiency was 0.499 on average and the highest (0.954) similarity was observed between 'Cheongdo-Bansi' and 'Haman-Bansi'. The similarity was lowest (0.192) between 'Damopan'and 'Atago'. Identification of each cultivar with the execption of 'Cheongdo-Bansi' and 'Gyeongsan-Bansi' was possible based on the SSR fingerprints, suggesting that these SSR markers are a useful tool for protecting intellectual property on newly developed cultivars.