Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Life Science
Journal Basic Information
Journal DOI :
Korean Society of Life Science
Editor in Chief :
Volume & Issues
Volume 20, Issue 12 - Dec 2010
Volume 20, Issue 11 - Nov 2010
Volume 20, Issue 10 - Oct 2010
Volume 20, Issue 9 - Sep 2010
Volume 20, Issue 8 - Aug 2010
Volume 20, Issue 7 - Jul 2010
Volume 20, Issue 6 - Jun 2010
Volume 20, Issue 5 - May 2010
Volume 20, Issue 4 - Apr 2010
Volume 20, Issue 3 - Mar 2010
Volume 20, Issue 2 - Feb 2010
Volume 20, Issue 1 - Jan 2010
Selecting the target year
Enhanced Production of Gellan by Sphingomonas paucibilis NK-2000 with Shifts in Agitation Speed and Aeration Rate after Glucose Feeding into the Medium
Lee, Nam-Kyu ; Seo, Hyung-Phil ; Cho, Young-Bai ; Son, Chang-Woo ; Gao, Wa ; Lee, Jin-Woo ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 811~818
DOI : 10.5352/JLS.2010.20.6.811
Optimal agitation speed and aeration rate for the production of gellan by Sphingomnas paucibilis NK2000 in a 7 l bioreactor were found to be 400 rpm and 1.0 vvm. The best time for glucose feeding into the medium for enhanced production of gellan by S. paucibilis NK2000 was 36 hr after cultivation. The concentrations of gellan produced by S. paucibilis NK2000 from 1) 20.0 g/l glucose without additional feeding, 2) 20.0 g/l glucose with feeding of 200.0 g/l glucose at 36 hr, in which the final concentration in the medium was 10.0 g/l, 3) 20 g/l glucose with feeding of 200.0 g/l glucose and a shift in an agitation speed from 400 to 600 rpm, 4) 20.0 g/l glucose with feeding of 200.0 g/l glucose at 36 hr and shifts in an agitation speed from 400 to 600 rpm and an aeration rate from 1.0 to 1.5 vvm, 5) and 20.0 g/l glucose with feeding of 200.0 g/l glucose at 36 hr and shifts in an agitation speed from 400 to 600 rpm and an aeration rate from 1.0 to 2.0 vvm, were 5.19, 5.74, 6.73, 7.93, and 9.40 g/l, respectively, and their conversion rates from glucose were 26.0, 19.1, 22.4, 26.4, and 31.3%, respectively. Compared to those developed using a normal process, production of gellan by S. paucibilis NK2000 from 20.0 g/l glucose was 1.81 times higher, and and its conversion rate was 1.20 times higher when the optimized process developed in this study was used.
Genetic Diversity and Phylogenetic Relationship of Genus Phyllostachys by RAPD Markers
Lee, Song-Jin ; Huh, Man-Kyu ; Shin, Hyun-Cheol ; Huh, Hong-Wook ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 819~824
DOI : 10.5352/JLS.2010.20.6.819
Genus Phyllostachys is a long-lived woody species primarily distributed throughout South East Asia. Many species of this genus has been regarded as medically and ecologically important in the world. We evaluated representative samples of the four taxa with RAPD to estimate genetic relationships within the genus Phyllostachys. The percentages of polymorphic loci were 8.9-33.3% at the species level. P. bambusoides was found to show lower genetic diversity (H=0.018) than other species. Total genetic diversity (
) was 0.315, genetic diversity within populations (
) was 0.043, the proportion of total genetic diversity partitioned among populations (
) was 0.659 and the gene flow (Nm) was 0.0263. As some Korean populations were isolated and patchily distributed, they exhibited low levels of genetic diversity. The four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic. P. nigra var. henonis. Stapf was found to be more closely related to P. pubescens than to P. nigra. P. bambusoides was quite distinct from the remaining species.
A Study on the Prolactin Receptor 3 (PRLR3) Gene and the Retinol-binding Protein 4 (RBP4) Gene as Candidate Genes for Growth and Litter Size Traits of Berkshire in Korea
Do, Chang-Hee ; Kim, Seon-Ku ; Kang, Han-Suk ; Shin, Teak-Soon ; Lee, Hong-Gu ; Cho, Seong-Keun ; Do, Kyung-Tak ; Song, Ji-Na ; Kim, Tae-Hun ; Choi, Bong-Hwan ; Sang, Byung-Chan ; Joo, Yeong-Kuk ; Park, Jun-Kyu ; Lee, Sung-Hoon ; Lee, Jeong-Ill ; Park, Jeong-Suk ; Sin, Young-Soo ; Kim, Byung-Woo ; Cho, Byung-Wook ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 825~830
DOI : 10.5352/JLS.2010.20.6.825
Two diallelic markers at candidate gene loci, the prolactin receptor 3 (PRLR3) gene and the retinol-binding protein 4 (RBP4) gene were evaluated for their association with growth and litter size traits in Berkshire. Genetic evaluation was conducted for 5,919 pigs with pedigree information, which included 3,480 growth performance records and 775 litter size records of 224 sows. From the same herd, genotyping was carried out on 144 and 156 animals for PRLR3 and RBP4, respectively. After assigning a genotype to subjects in which both parents had a homozygous genotype, numbers of genotyped animals increased to 474 and 338, for the PRLR3 gene and RBP4 gene, respectively. The genotype effects of two markers were estimated with breeding values of the genotyped animals. The additive effects of total number of piglets born and number of piglets born alive in the PRLR3 locus were -0.28 and -0.13, respectively. The dominance effect of the RBP4 locus on average daily gain was -10.58 g. However, the polymorphism of the RBP4 locus in total number of piglets born and number of piglets born alive has shown -0.34 and -0.33 of the additive genetic effects. In view of the results, MAS (marker-assisted selection) favoring B alleles of RBP4 and PRLR3 loci could potentially accelerate the rate of the genetic improvement in the litter size traits.
Pharmacological Characterization of KR-31125, a Novel Nonpeptide AT1 Receptor Antagonist
Lee, Sung-Hou ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 831~837
DOI : 10.5352/JLS.2010.20.6.831
KR-31125 (2-butyl-5-dimethoxymethyl-6-phenyl-7-methyl-3-[[2'-(1H-tetrazol-5-yl)biphenyl-4-yl]methyl]-3H-imidazo[4,5-b]pyridine) is a potent inhibitor of angiotensin II type 1 (
) receptors in human recombinant
receptors and rabbit aorta. These in vitro studies revealed that KR-31125 inhibited specific [
]-angiotensin II binding to human recombinant
receptors in a concentration dependent manner with an
nM. However, no interaction with
receptors was detected as displayed by the competition binding of [
] CGP 42112A to human recombinant
receptor. The binding action was also confirmed as a competitive mode that was identical to the previously studied compound, losartan. In addition, KR-31125 caused a nonparallel shift to the right in the concentration response curves to angiotensin II with a 30-80% decrease in the maximum contractile responses (
: 7.63). Compared to the previous studies with losartan that showed a parallel right shift in the maximum contractile responses to AII (
: 7.59), KR-31125 presented a different mode of action with a similar potency to losartan. These results demonstrate that KR-31125 is a highly potent and
selective angiotensin II receptor antagonist that can be applied to the fields of new diagnostic and research tools with upcoming in vivo study results.
Purification and Biochemical Characteristics of Fibrinolytic Enzyme from Streptomyces corcohrussi JK-20
Kim, You-Jung ; Park, Jeong-Uck ; Seo, Min-Jeong ; Kim, Min-Jeong ; Lee, Hye-Hyeon ; Jin, Se-Hun ; Kang, Byoung-Won ; Choi, Yung-Hyun ; Jeong, Yong-Kee ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 838~844
DOI : 10.5352/JLS.2010.20.6.838
A fibrinolytic enzyme of Streptomyces corcohrussi from soil sediment was purified by chromatography using DEAE-Sephadex A-50 and Sephadex G-50. The analysis of SDS-polyacrylamide gel suggested that the purified enzyme is a homogeneous protein and the molecular mass is approximately 34 kDa. The purified enzyme showed activity of 0.8 U/ml in a plasminogen-rich fibrin plate, while its activity in a plasminogen-free fibrin plate was only 0.36 U/ml. These results suggested that the purified enzyme acts as a plasminogen activator. The fibrinolytic activity of the enzyme under the supplementation of protease inhibitors,
-ACA, t-AMCHA and mercuric chloride in the enzyme reaction was less than 24%, indicating that it could be modulated by the plasmin and/or fibrinogen inhibitors involved in the fibrinogen-to-fibrin converting process. As time passed,
, a heavy metal ion, inhibited the activity to 34.1%. The optimum temperature of the purified enzyme was approximately
and over 92% of the enzyme activity was maintained between pH 5.0 and 8.0. Therefore, our results provide a potential fibrinolytic enzyme as a noble thrombolytic agent from S. corcohrussi.
Role of Alveolar Macrophages in Productions of Prostaglandin D
in the Inflamed Lung
Joo, Myung-Soo ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 845~852
DOI : 10.5352/JLS.2010.20.6.845
Our previous study showed that lungs infected by Pseudomonas, a gram-negative bacteria, produce prostaglandin
) and prostaglandin
), the two major prostanoids generated by cyclooxygenase-2 (COX-2), and that the ratio of
can affect the outcome of the bacterial lung infection. In this study, we sought to uncover the mechanism that determines the ratio of
produced in lung inflammation. When treated with lipopolysaccharide (LPS), primary alveolar macrophages, extracted from mouse lung, more
was produced than
, whereas MH-S, a murine alveolar macrophage cell line, produced more
in a similar experiment. Western blot analyses showed that the kinetics of COX-2 expression in both cell types is similar and epigenetic silencing of COX-2 expression did not affect expressions of lipocalin-PGD synthase (L-PGDS) and PGE synthase (mPGES-1), major enzymes synthesizing
in inflammation, respectively, indicating no effect of COX-2 on expressions of the two enzymes. Expressions of L-PGDS and mPGES-1 were also similar in both cell types, suggesting no effect of the two key enzymes in determining the ratio of
in these cells. A single intraperitoneal injection of LPS to C57BL/6 mice induced COX-2 expression and, similar to alveolar macrophages, produced more
in the lung. These results suggest that the differential expressions of
in the lung reflect those in alveolar macrophages and may not be directly determined by the enzymes responsible for
Inactivation of the DevS Histidine Kinase of Mycobacterium smegmatis by the Formation of the Intersubunit Disulfide Bond
Lee, Jin-Mok ; Park, Kwang-Jin ; Kim, Min-Ju ; Ko, In-Jeong ; Oh, Jeong-Il ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 853~860
DOI : 10.5352/JLS.2010.20.6.853
The DevSR two-component system is a major regulatory system involved in redox sensing in Mycobacterium smegmatis. The DevSR system consists of the DevS histidine kinase and its cognate DevR response regulator. When exposed to hypoxic conditions, the DevS histidine kinase is activated to phosphorylate the DevR response regulator, leading to the transcriptional activation of the DevR regulation. The ligand-binding state of the heme embedded in the N-terminal GAF domain of DevS determines the kinase activity of DevS. In this study, we demonstrated that the redox-responsive cysteine (C547) in the C-terminal kinase domain is involved in the redox-dependent control of DevS kinase activity. The formation of an intersubunit disulfide bond between the C547 residues in the presence of
led to inactivation of DevS kinase activity. The reduction of the oxidized DevS with reductants such as
-mercaptoethanol and dithiothreitol resulted in the restoration of DevS kinase activity. It was demonstrated in vivo by complementation test that the substitution of C547 to alanine partially impaired the sensory function of DevS in M. smegmatis.
Impacts of Bioactive Components Addition on Qualities of Imitation Crab Meat Containing Spent Laying Hen Meats During Storage
Jin, Sang-Keun ; Hur, Sun-Jin ; Shin, Teak-Soon ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 861~869
DOI : 10.5352/JLS.2010.20.6.861
Imitation crab stick samples were made from Alaska pollack with protein recovered from spent laying hens breast meat (C), Alaska pollack with spent laying hens breast recovered protein and cordyceps powder (T1), Alaska pollack with spent laying hens breast recovered protein and silkworm cocoon powder (T2), and Alaska pollack with spent laying hens breast recovered protein and conjugated linoleic acid (CLA) (T3). There were no significant differences in moisture, crude protein and crude fat among the imitation crab samples. pH was significantly higher in T2 and T3 compared with C and T1 after 4 weeks of storage. The values of
, and W (whiteness) in T3 were found to be more stable during storage and W in T3 was significantly higher than other samples. T3 was significantly lower in hardness, brittleness, gumminess and adhesiveness after 4 weeks of storage. Thiobarbituric acid reactive substance (TBA) values decreased with storage, whereas volatile basic nitron (VBN) values increased with storage periods in all samples. In sensory evaluation, there were no significant differences in color, odor, taste, tenderness, juiciness and overall acceptability among the samples. T3 was significantly lowest in quality among the treated samples.
The Effect of Kinesio Taping on Muscle Strength Improvement, Blood Fatigue Factors, Muscle Fatigue and Damage Index
Lee, Min-Sun ; Paik, Il-Young ; Kwak, Yi-Sub ; No, Hee-Tae ; Jin, Hwa-Eun ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 870~876
DOI : 10.5352/JLS.2010.20.6.870
The purpose of this study was to examine the effect of kinesio taping on muscle strength and changes of muscle fatigue and damage. 10 male subjects participated in 1-RM and isometric cybex muscle strength tests with and without taping application. Muscle strength (bench press, leg press) and extension (knee, shoulder) strength were significantly increased after taping, but there was no significant difference in flexion (knee, shoulder) strength. The concentration of fatigue factors (ammonia, phosphorous), muscle damage index substances (CK, LDH), IGF-I and creatinine were reduced after taping, but there were no significant differences.
Growth Inhibitory and Quinone Reductase Activity Stimulating Effects of Internal Organs of Aplysia kurodai Fractions on Cancer Cell Lines In vitro
Shin, Mi-Ok ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 877~884
DOI : 10.5352/JLS.2010.20.6.877
We investigated the growth inhibitory effect of internal organs of Aplysia kurodai (AK) on proliferation in cancer cell lines in vitro. The internal organs of AK were extracted with methanol (AKM), which were then further fractionated into four subfractions by using solvent partition method, resulting in hexane (AKMH), methanol (AKMM), butanol (AKMB), and aqueous (AKMA) soluble fractions. We determined the cytotoxic effect of these four fractions in four kinds of cancer cell lines - HepG2, MCF-7, HT29 and B16-F10 - by MTT assay. Among the four subfractions of AKM, AKMM showed the strongest cytotoxic effects on all cancer cell lines which were used. Morphological changes such as membrane shrinking and blebbing of cells were also observed in AKMM treatment in HepG2 cells. In addition, we also observed quinone reductase (QR) induced effect in the methanol layer (AKMM) of HepG2 cells. AKMM showed the highest induction activity of quinone reductase on HepG2 cells among the partition layers. The QR induced effect of AKMM was determined to be 2.4 at
level with a control value of 1.0. Although further studies are needed, the present work suggests that internal organs of Aplysia kurodai (AK) may be a chemopreventive agent for the treatment of human cells.
Effects of Complex Training on Cardiovascular Risk Factors in Middle Aged Obese Women
Won, Ho-Sam ; Yang, Joung-Ok ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 885~893
DOI : 10.5352/JLS.2010.20.6.885
This study measured physical characteristics and physical composition of 16 middle-aged obese women using a body composition analyzer. TC, TG, HDL-C and LDL-C were investigated in order to analyze components of serum liquids. The study also compared changes regarding cardiovascular risk factors before and after a 12-week exercise program by measuring insulin and intensity of TNF-
. To examine such changes, the study carried out a 12-week, complex training program for middle-aged obese women who did not regularly exercise. The results of the study were as follows: 1) after 12 weeks of complex training, in changes of physical characteristics, weight, BMI and WHR were significantly reduced. Systolic blood pressure and diastolic blood pressure increased, but there were no significant differences; 2) after 12 weeks of complex training, in changes of physical composition, body fat mass and body fat rate were significantly reduced and fat free mass was significantly increased; 3) after 12 weeks of complex training, in changes of serum liquids, TC, TG and LDL-C were significantly reduced. HDL-C increased, but this was not statistically significant; 4) after 12 weeks of complex training, in changes of cardiovascular risk factors, insulin and TNF-
were significantly reduced.
Obstetrical & Gynecological Health Status and Body Composition of Married Immigrant Women in Busan
Kim, Dong-Hee ; Park, Hyoung-Sook ; Bae, Kyung-Eui ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 894~901
DOI : 10.5352/JLS.2010.20.6.894
The purpose of this study was to examine obstetrical & gynecological health status and body composition for married female immigrants. The participants were 154 immigrant women living in Busan. Data were collected from July 2008 to July 2009 using a questionnaire and a bioelectrical impedance analysis tool (Inbody 720) for analysis of body composition. Most subjects were in the normal range for body composition, but some of those who answered the questionnaire had experienced some gynecological problems such as dysmenorrhea, or irregular menstruation. There was a significant difference in experiences with in vitro fertilization, pregnancy and abortion according to age and in premensturaion syndrome, as well as differences in regulation of menstruation during the past year, leukorrhea odor, experiences in in vitro fertilization, and delivery and breastfeeding according to nationality. There were significant differences in body mass index and waist-hip ratio according to age, as well as differences in percent body fat, body mass index and waist-hip ratio according to nationality. There were also significant differences in body mass index and waist-hip ratio according to period of stay in Korea. Procreative care protocols concerning married female immigrants' characteristics should be developed.
Culture Conditions of Geobacillus kaustophilus DSM 7263 for Production of Thermophilic Extracellular Lipase
Jeon, Sung-Jong ; Kang, Hyun-Woo ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 902~906
DOI : 10.5352/JLS.2010.20.6.902
A producer of thermophilic extracellular lipase, Geobacillus kaustophilus DSM 7263, was selected from various microorganisms of the Geobacillus genus. We investigated optimum conditions for mass production of G. kaustophilus lipase. Among the different natural oil media, olive oil was optimal for enzyme production. The maximum amount of enzyme production was obtained when G. kaustophilus was grown in a medium containing 0.5% olive oil as a carbon source. The pH and temperature for optimal growth were pH 8.0 and
, respectively, while the optimum pH and temperature for lipase production were pH 6.0 and
, respectively. In the presence of
, lipase production was dramatically enhanced by 247% and 157%, respectively, whereas enzyme production was inhibited by
. The addition of 0.1% (v/v) triton X-100 increased lipase production and cell growth when compared to the negative control.
Genetic Characterization for Human Enterovirus Isolated from Busan Region in Korea
Kim, Nam-Ho ; Min, Sang-Kee ; Park, Eun-Hee ; Park, Yon-Koung ; Kwan, Sun-Mok ; Jin, Seong-Hyeon ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 907~913
DOI : 10.5352/JLS.2010.20.6.907
Human enteroviruses (HEV) are considered one of the major infectious causes of central nervous system infections such as aseptic encephalomeningitis in pediatrics. This study was focused on providing information related to genetic characteristics and diversities of HEV which prevailed between 2007 and 2009 in Busan, Korea. A total of 2,743 specimens were collected from children and screened for isolation of HEV by cell culture and RT-PCR. Among the specimins, 240 isolates were grouped into 21 different HEV serotypes using VP1 RT-PCR. The major etiological agents were CV-A6 and CV-B2 in 2007, E-6 and E-30 in 2008 and CV-B1 in 2009. The occurrence of HEV infections was the most frequent in the summer (May to August, 188 cases, 78.3%). Most of the isolates were identified from specimens from children under 10 years old, with the highest occurrence in the 2 to 4 year old range (15.2%). However, there were no significant differences between male and female children for the isolates. For analyzing genetic characterization, VP1 gene was amplified by RT-PCR and sequenced. The phylogenetic tree was established by Clustal W method using DNASTAR software. Using the sequence analysis of the VP1 region, it was classified into 2 groups; HEV-A and HEV-B. The HEV-A group contained 6 serotypes and sequences of 31 isolates were compared within each serotype. The HEV-B group contained 10 serotypes and the sequences of 41 isolates were compared within each serotype. Homology analysis of the VP1 region showed that the identity scores of HEV-A and B isolates were different. In conclusion, genetic divergences were observed among the isolates from children between 2007 and 2009 in Busan.
Pathological Effect of Melatonin on Vascular Endothelial Cell Detachment
Seo, Jeong-Hwa ; Kim, Sung-Hyen ; Ahn, Sun-Young ; Jeong, Eun-Sil ; Cho, Jin-Gu ; Park, Heon-Yong ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 914~921
DOI : 10.5352/JLS.2010.20.6.914
In this study, we carried out a series of experiments to know whether melatonin, an anti-oxidative and immunosuppressive agent, played an important role in endothelial cells. It was revealed that melatonin had little or no effect on endothelial proliferation, cell death or migration. Additionally, melatonin had no effect on adhesion of THP-1 leukocytes to bovine aortic endothelial cells (BAECs) and THP-1 homotypic cell aggregation. In contrast, it was shown that melatonin diminished the basal level of nitric oxide by PP2A-mediated dephosphorylation of endothelial nitric oxide synthase (eNOS), leading to enhanced detachment of BAEC from the extracellular matrix. Collectively, melatonin in high doses decreases the NO production via regulations of PP2A and eNOS activities, inducing detachment of endothelial cells, a possible initial step for thrombosis.
Evaluation of In-vitro Anticoagulation Activity of 33 Different Medicinal Herbs
Ryu, Hee-Young ; Ahn, Seon-Mi ; Kim, Jong-Sik ; Sohn, Ho-Yong ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 922~928
DOI : 10.5352/JLS.2010.20.6.922
This study was carried out to develop safe and novel anticoagulation agents from oriental medicinal herbs. From 33 medicinal herbs, 40 different ethanol extracts were prepared according to place of origin or extraction parts, and anticoagulation activities were evaluated by determination of thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT). The average water content and average extraction ratio for the medicinal herbs were
, respectively. Evaluation of TT at various concentrations of the extract led to the selection of Mucuna birdwoodiana, Prunus armeniaca, Cacalia ainsliaeiflora, Cinnamonum aromaticum, and Rhus javanica Linneas potent antithrombosis medicinal herbs. Evaluation of PT and aPTT showed that the extracts of R.javanica Linne, M. birdwoodiana, and P. armeniaca have strong anticoagulation activities. Determination of hemolytic activities of 40 different ethanol extracts against human red blood cells, however, showed that only M. birdwoodiana, C. ainsliaeiflora, C. aromaticum, and R. javanica Linnehas strong anticoagulation activity without hemolytic activity at a concentration of 500 mg/ml. Our results suggest that oriental medicinal herbs, which are under a mass-production system, have potentialas a safe and novel source of anticoagulants, as well being a thrombin-specific and coagulation factor-specific inhibitor.
Cloning and Identification of Differentially Expressed Genes Induced by Fungal Infection from Silkworm, Bombyx mori
Lee, Jin-Sung ; Hong, Su-Young ; Lee, Ki-Hwa ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 929~933
DOI : 10.5352/JLS.2010.20.6.929
We tried to identify differentially expressed genes (DEGs) from a silkworm, Bombyx mori, involved in fungal (Aspergillus niger) infection. A total RNA purified from fungal-induced and normal B. mori (
instar larvae) was used for the cDNA synthesis. Differentially expressed genes were screened by annealing control primer (ACP)-based PCR technique. Comparing the gene expression profiles between fungal infection and control silkworm, we detected 10 genes that were differentially expressed in fungal induction and performed molecular cloning and nucleotide sequencing of the 10 genes. We confirmed the expression patterns of 3 DEGs by RT-PCR. The 3 DEGs over-expressed in fungal infection were identified as lysozyme, enbocin and an unknown gene. They were first identified to be genes induced by fungal infection. Although the detailed functions of 3 genes and their products remain to be determined, the genes will provide insight into the molecular mechanisms of insect-immune systems induced by fungal infection.
Improvement of Antibacterial Activities of Bacteriocidal Yeasts Using the GPD Promoter
Jang, Min-Kyung ; Yu, Ki-Hwan ; Kim, Nam-Young ; Lee, Ok-Hee ; Shin, Jae-Kyun ; Jang, Hye-Ji ; Lee, Seung-Woo ; Lee, Dong-Geun ; Lee, Sang-Hyeon ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 934~939
DOI : 10.5352/JLS.2010.20.6.934
We have previously reported recombinant productions of bacteriocins using yeast expression plasmid pAUR123, which contains the alcohol dehydrogenase (ADH) promoter, in Saccharomyces cerevisiae cells and their antibacterial activities. In order to improve the antibacterial activities of bacteriocidal yeast cells, a strong glyceraldehyde phosphate dehydrogenase (GPD) promoter gene of S. cerevisiae was amplified and inserted upstream into bacteriocin genes such as the OR-7, Subpeptin JM4-A or JM4-B gene in the corresponding recombinant yeast plasmid. Yeast cells transformed by the recombinant plasmid containing the GPD promoter represented higher antibacterial activities against both Gram positive B. subtilis and Gram negative E. coli cells compared to those transformed by the corresponding recombinant plasmid containing the ADH promoter. Thus, yeast cells harboring the recombinant plasmid containing the GPD promoter constructed in this study could be applied in the food preservative or animal feed industries.
Tyrosinase Inhibition Activity and Antioxidant Capacity by Fermented Products of Some Medicinal Plants
Cha, Jae-Young ; Yang, Hyun-Ju ; Jeong, Jae-Jun ; Seo, Won-Seok ; Park, Jun-Seok ; Ok, Min ; Cho, Young-Su ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 940~947
DOI : 10.5352/JLS.2010.20.6.940
The effects of fermented products from 40 medicinal herbals commonly available in Korea were examined according to concentrations of polyphenolic compound and kojic acid, and the activities of DPPH (
-picrylhydrazyl) free radical scavenging and tyrosinase. The polyphenolic compound concentrations were 0.24 by Corydalis turtschaminovill ~ 11.42% (dry matter basis) by Syringa velutina in the extracts and 0.18 by Poria cocos ~ 12.27% by S. velutina in the fermented products. Kojic acid concentrations were 0.02 by Poria cocos Sclerotium ~ 9.67 mM by S. velutina in the extracts and 0.33 by P. cocos ~ 10.32 mM by S. velutina in the fermented products. Syringa velutina contained the highest polyphenolic compound and kojic acid concentrations, which were higher in the fermented product than in the extract. Higher DPPH free radical scavenging activity (>60%) was observed in the extracts of A. sessiliflorum, Citrus nobillis, and Angelica gigas and the fermented product of A. sessiliflorum compared to the other medicinal plants. Higher tyrosinase inhibition activity (>50%) was observed in the extracts of Morus alba, Glycyrrhiza glabra, and Rubus coreanus and the fermented products of G. glabra, Cnidium officinale, and S. velutina. Based on the above results, G. glabra, C. officinale, and S. velutina possessed high tyrosinase-inhibitive activities and kojic acid concentrations, which could be definitely enhanced by the fermentation of Phenillus linteus mycelium.
Effect of Aerobic Exercise on Factors Relative to the Brain Nerve Growth in Girls
Pyun, Mi-Young ; Cho, Han-Sam ; Jeon, Jae-Young ; Kim, Jong-Won ; Lee, Kyung-Hee ; Lim, Chun-Kyu ; Kim, Tae-Un ; Kim, Hyun-Jun ; Kwak, Yi-Sub ; Ko, Ki-Jun ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 948~954
DOI : 10.5352/JLS.2010.20.6.948
Exercise can favorably influence brain plasticity by facilitating neurogeneration, neuroadaptivity, and neuroprotection. Aerobic exercise has been reported to change brain nerve growth factors (growth hormone, insulin like factor-1, estrogen and serotonin). The purpose of this study was to demonstrate the effects of aerobic exercise for 12 weeks on brain nerve growth factors in girls. Fourteen female participants in elementary school grades 1 through 3 were randomly allocated to the exercise group (EG, n=6) and control group (CG, n=8). The EG participated in 60 minutes of modified ballet exercise as aerobic training three days a week for 12 weeks. Based on comparison between groups by two-way ANOVA with repeated measures, aerobic exercise program participants experienced decreased weight (p<0.01), BMI (p<0.01), fat mass (p<0.001), fat percent (p<0.001) and increased LBM (lean body mass) percent (p<0.001). In addition, we detected that aerobic exercise decreased the level of serotonin (p<0.05) and increased the level of GH (p<0.05) and IGF-1 (p<0.05). These findings suggest that aerobic exercise programs can be an efficient intervention to change body composition, alleviate central fatigue, improve brain function, and induce brain cell proliferation in girls.
Multiplex PCR-aided Differential Diagnosis of Taeniid Species
Lee, Hye-Jung ; Seo, Min ; Kwak, Sahng-June ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 955~959
DOI : 10.5352/JLS.2010.20.6.955
Differential diagnosis of the taeniid proglottids between Taenia asiatica and T. sagniata is a daunting task due to their close morphological similarity. However, to correctly diagnose them on time is important in managing infected patients, as well as for reducing serious complications such as cysticercosis. Currently, DNA-based methods for the dissection of genomic information of parasites are being employed to make accurate and rapid diagnoses in the field of parasitology. In this study, multiplex PCR was established and exploited to identify exact species of taeniid adult worms recovered from Korean people. To discriminate one from the other other, primers-Ta4978F, Ts5058F, Tso7421F, and Rev7915- were used for the multiplex PCR, which provided swift and precise identification of the taeniid worms being observed. Also,having instituted PCR methodology, we ascertained that easiness would be achieved to reassess and re-evaluate Korean endemic data on human taeniid cestodes.
Analysis of Anti-Allergic Activities by Chaff Vinegar Liquor
Lee, Sang-Han ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 960~963
DOI : 10.5352/JLS.2010.20.6.960
To evaluate whether chaff vinegar liquor (CVL) has potential in ameliorating allergic symptoms in mice, we tested the anti-allergic activities with a DNFB (dinitrofluorobenzene)-induced allergic mouse model. The DNFB-induced allergic symptoms were reduced by 20% compared to the control by chaff vinegar liquor supplemented with Perilla frutescens var. acuta. Allergic symptoms were examined by measuring the width of epidermis swelling. The hematoxyline & eosin (H&E) staining also revealed that the chaff vinegar liquor dramatically decreased the allergic symptoms in the epidermis of the ear. These results collectively suggest that the chaff vinegar liquor supplemented with P. frutescens var. acuta has potential in ameliorating allergic symptoms, showing that the liquor could be a useful biomaterial(s) for the nutraceutical or cosmetic industries.
Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Escherichia coli Isolated from Diseased Animals in Korea
Shin, Dong-Ho ; Kim, Ha-Young ; Byun, Jae-Won ; Kim, Dae-Keun ; Lim, Suk-Kyung ; Jung, Byeong-Yeal ;
Journal of Life Science, volume 20, issue 6, 2010, Pages 964~967
DOI : 10.5352/JLS.2010.20.6.964
Plasmid-mediated quinolone resistance (PMQR) determinants have been contributed to quinolone resistance of gram-negative bacteria worldwide. However, little data on the prevalence of these determinants in bacteria from animals are available in Korea. In this study, the prevalence of PMQR genes was investigated with E. coli originating from diseased animals. Among 55 E. coli tested, 11 showed PMQR genes by PCR. The most prevalent genotype was qepA (14.5%), followed by aac(6')-Ib-cr (7.3%) and qnrS (1.8%). Interestingly, two isolates with PMQR genes did not show quinolone resistance in this study. The isolates exhibited higher fluoroquinolone resistance in aac(6')-Ib-cr in combination with qnrS or qepA compared with aac(6')-Ib-cr only. In a conjugal transfer test, PMQR genes were transferred from donor to recipient.