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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 20, Issue 12 - Dec 2010
Volume 20, Issue 11 - Nov 2010
Volume 20, Issue 10 - Oct 2010
Volume 20, Issue 9 - Sep 2010
Volume 20, Issue 8 - Aug 2010
Volume 20, Issue 7 - Jul 2010
Volume 20, Issue 6 - Jun 2010
Volume 20, Issue 5 - May 2010
Volume 20, Issue 4 - Apr 2010
Volume 20, Issue 3 - Mar 2010
Volume 20, Issue 2 - Feb 2010
Volume 20, Issue 1 - Jan 2010
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Spatial Autocorrelation Analysis of Carex humilis on Mt. Giri by RAPD
Lee, Bok-Kyu ; Lee, Byeong-Ryong ; Huh, Man-Kyu ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1287~1293
DOI : 10.5352/JLS.2010.20.9.1287
The spatial distribution of alleles and geographical distances of a Carex humilis population on Mt. Giri in Korea were studied. A total of 102 DNA fragments (bands) were found among 107 plants. Among these 102 bands, 48 (47.1%) bands were polymorphic. In a simple variability of subpopulations by the percentage of polymorphic bands, distances I and V exhibited the lowest variation (16.7%). Distance VIII showed the highest variation (22.6%). The total genetic diversity (H) was 0.076 across species. Class VIII had the highest H (0.093), while class I had the lowest (0.063). Genetic similarity of individuals was found among subpopulations at up to a scale of 60 m distance, and this was partly due to a combination of alleles. Within the Mt. Giri population, a strong spatial structure was observed for RAPD markers, indicating a very low amount of migration among subpopulations and that the distribution of individual genotypes of a given population was clumped. The present study demonstrated that analysis of RAPD markers could be successfully used to study the spatial and genetic structures of C. humilis.
Experimental Infection for the Neutralization of White Spot Syndrome Virus (WSSV) in Wild Captured Sand Shrimp, Crangon affinis
Gong, Soo-Jung ; Kim, Yeong-Jin ; Choi, Mi-Ran ; Kim, Sung-Koo ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1294~1298
DOI : 10.5352/JLS.2010.20.9.1294
White spot syndrome virus (WSSV) is one of the most virulent viral agents threatening the penaeid shrimp culture industry. This study was carried out to evaluate the susceptibility of the sand shrimp, Crangon affinis, to WSSV as an alternative experimental model. WSSV caused 100% mortality in C. affinis within 7 days after experimental infection by immersion. Based on challenge studies, it was confirmed that C. affinis could be a potential host in WSSV transmission. Also, the neutralization of WSSV was carried out using an antiserum raised against recombinant envelop protein rVP466 to evaluate the WSSV infection mechanism. A constant amount of WSSV (at
diluted stocks) was incubated with various amounts of antiserum and then mixed to 20 l reservoir for the immersion challenge of C. affinis for neutralization. At 5 days post challenge, the shrimp in the positive control immersed in the immersion reservoir containing WSSV stock showed 100% mortality. The shrimps challenged with the 3 different mixtures of WSSV and rVP466 antiserum (1:0.1, 1:0.5 and 1:1) showed 100%, 68.8% and 68.8% mortality at 14 days post challenge, respectively. These results indicated that the antiserum raised against rVP466 could block WSSV infection in C. affinis. Therefore, this study confirmed that C. affinis can be naturally infected by WSSV as another potential host and that C. affinis can be used as an alternative experimental animal instead of penaeid shrimps.
Relationship and Clinical Usefulness between Preoperative Levels of Brain Natriuretic Peptide, Other Cardiac Markers and Perioperative Parameters in Patients with Coronary Artery Disease
Choi, Seok-Cheol ; Kim, Yang-Weon ; Hyun, Kyung-Yae ; Hwang, Soo-Myung ; Moon, Seong-Min ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1299~1305
DOI : 10.5352/JLS.2010.20.9.1299
Cardiac troponin-I (cTnI), creatine kinase-MB (CK-MB), and C-reactive protein (CRP) are routine cardiac markers for the diagnosis of cardiovascular disease. Recently, brain natriuretic peptide (BNP) has garnered attention as a marker of heart failure. This study was retrospectively designed to investigate the relationships between preoperative BNP, other cardiac markers levels and perioperative parameters in seventy-four adult patients that underwent off-pump coronary artery bypass grafting (OPCAB) and to assess its usefulness for predicting postoperative outcomes. Preoperative levels of BNP, cTnI, CK-MB, and CRP had significantly positive or negative correlations with echocadiographic parameters. There were significantly positive relationships between BNP, cTnI, CK-MB, and CRP concentration. Postoperative mechanical ventilation time had a positive correlation to preoperative levels of cTnI, CK-MB, and CRP, while ICU-staying period had a positive correlation with BNP, cTnI and CK-MB. These results reveal that a preoperative level of BNP is a good predictor and that its combination with cTnI, CK-MB, and CRP might be useful for diagnosis and comprehensive risk stratification of patients with coronary heart diseases, as well as prognosis of perioperative outcomes in OPCAB patients.
Cloning and Characterization of a Novel Carboxylesterase Gene from Cow Rumen Metagenomic Library
Asraful Islam, Shah Md. ; Kim, Min-Keun ; Renukaradhya, K. Math ; Srinivasa, Reddy R.N. ; Kim, Eun-Jin ; Kim, Jung-Ho ; Kim, Hoon ; Yun, Han-Dae ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1306~1313
DOI : 10.5352/JLS.2010.20.9.1306
The gene encoding esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est1R) was 2,465 bp in length, encoding a protein of 366 amino acid residues, and the molecular weight of the enzyme was 61,166 Da. Est1R of rumen cosmid library shared 5.9% amino acid identity with Est1R (P37967) of PNB carboxylesterase, 6.1% with Est1R (1EEAA) of acetylcholinesterase and 6.1% with Est1R (1H23A) of chain A. BlastP in NCBI database analysis of Est1R revealed that it was not homologous to previous known lipases and esterases. Est1R showed optimum activity at pH 7.0 and
. On the other hand, the enzyme was found to be most active without organic solvent, followed by 95% activity with methanol, and the enzyme activity was highly affected by hexane (lost 51% activity). Therefore, the novel esterase gene est1R is likely obtainable from cow rumen metagenome and may be utilized for industrial purposes.
Construction and Analysis of Binary Vectors for Co-Overexpression, Tissue- or Development-Specific Expression and Stress-Inducible Expression in Plant
Lee, Young-Mi ; Park, Hee-Yeon ; Woo, Dong-Hyuk ; Seok, Hye-Yeon ; Lee, Sun-Young ; Moon, Yong-Hwan ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1314~1323
DOI : 10.5352/JLS.2010.20.9.1314
In this study, we constructed various kinds of binary vectors with the pPZP backbone for co-overexpression, tissue- or development-specific expression and stress-inducible expression, and validated them for ectopic expression of target genes. Using a modified CaMV 35S promoter, a binary vector was generated for co-overexpression of two different genes and was confirmed to be efficient for overexpressing two different target genes at the same time and place. Binary vectors containing At2S3, KNAT1 or LFY promoters were constructed for tissue-specific or development-specific gene expression, and the binary vectors were suited for embryo/young seedling stage-, shoot apical meristem- or leaf primordia-specific expressions. Furthermore, the binary vectors containing RD29A or AtNCED3 promoters were validated as suitable vectors for gene expression induced by abiotic stresses such as high salt, ABA, MV and low temperature. Taken together, the binary vectors constructed in this study would be very useful for analyzing the biological functions of target genes and molecular mechanisms through ectopic expression.
Effects of Mifepristone and Tamoxifen on Calcium Modulation in DU-145 Prostate Cancer Cells
Kim, Yeo-Reum ; Kim, Byeong-Gee ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1324~1331
DOI : 10.5352/JLS.2010.20.9.1324
Mifepristone (MIF) and Tamoxifen (TAM) have been used in the treatment of prostate cancer and breast cancer for more than a decade. MIF can induce apoptosis in both AR-positive and negative prostate cancer cells. Because of its pleiotropic ligand-receptor properties, TAM exerts cytotoxic activity in estrogen (ER)-positive and various ER.negative cancer cells. However, the molecular mechanisms of these two substances are not yet clear. In the present work, we report that the cytotoxic effects of MIF and TAM are due to the modulation of intracellular
level in DU-145, androgen-insensitive cells. When the cells were treated with micromolar concentrations of either MIF or TAM, the growth and viability were significantly decreased in a dose- and time-dependent manner. The apoptosis induced by MIF or TAM was further proved and analyzed by confocal laser scanning microscopy (CLSM) and fluorescence-activated cell sorting (FACS). In the cells cultivated in a normal 1.5 mM
medium, both MIF and TAM also induced an increase of the intracellular
level in a dose-dependent fashion. Since a change in calcium level could not be found in cells of the
-free medium, the increase of intracellular
level might be due to an increase in extracellular calcium uptake. Our results show that the apoptotic effect was more prominent in TAM treatment compared to MIF treatment in DU-145 cells. The above findings might be due to the difference in the uppermost pathways of apoptosis induced by either MIF or TAM. When we checked the level of procaspase-8 activation, TAM showed minor level of activation, as opposed to MIF, which exerted strong activation. In both treatments, the levels of anti-apoptotic protein Bcl-2 decreased, and pro-apoptotic protein Bax level increased more than 2-fold. The activation of caspase-3, a key protease enzyme in the downstream pathway of apoptosis, was much higher in the cells treated with TAM, compared to the MIF treatment. The overall apoptotic activity shown in the present work was closely related to intracellular
concentration levels. Therefore, the cytotoxic activity induced by MIF and TAM might have been due to intracellular calcium modulation.
Transcriptional Regulation of Human GD3 Synthase (hST8Sia I) by Fenretinide in Human Neuroblastoma SH-SY-5Y Cells
Kang, Nam-Young ; Kwon, Haw-Young ; Lee, Young-Choon ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1332~1338
DOI : 10.5352/JLS.2010.20.9.1332
To elucidate the mechanism underlying the regulation of hST8Sia I gene expression in FenR-induced SH-SY5Y cells, we characterized the promoter region of the hST8Sia I gene. Functional analysis of the 5'-flanking region of the hST8Sia I gene showed that the -1146 to -646 region functions as the FenR-inducible promoter of hST8Sia I in SH-SY5Y cells. Site-directed mutagenesis indicated that the NF-&B binding site at -731 to -722 was crucial for the FenR-induced expression of hST8Sia I in SH-SY5Y cells. To investigate which signal transduction pathway was involved in FenR-stimulated induction of hST8Sia I in SH-SY5Y cells, we performed Western blot analysis using phospho-specific antibodies in order to measure their degree of regulatory phosphorylation. Phosphorylations of AKT and RelA (p65) subunit of NF-
were significantly elevated in cytosolic and nuclear fractions of FenR-stimulated SH-SY5Y cells, respectively, than in control or DMSO-treated SH-SY5Y cells. These results suggest that FenR induce transcriptional up-regulation of hST8Sia I gene expression through translocation of RelA (p65) subunit of NF-
to nucleus by AKT signal pathway in SH-SY5Y cells.
Production of Antifungal Compost by Using Bacillus licheniformis KJ-9
Seo, Dong-Cheol ; Ko, Jeong-Ae ; Lee, Sang-Won ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1339~1344
DOI : 10.5352/JLS.2010.20.9.1339
In order to produce environmental-friendly fermented compost, a cattle manure-sawdust compost (antifungal compost) was developed by inoculation of B. licheniformis KJ-9 to cattle manure-sawdust. The thermal stability of the antifungal substance produced by B. licheniformis KJ-9 maintained more than 60% antifungal activity with heat treatment at
for 10 min, and the optimum pH of antifungal activity of the substance was 7.0. In a pot experiment with red pepper, the antifungal compost increased 1.5~2 times in leaf number and stem and root growth rate compared to those of commercial compost. Also, the diameter of stems increased 1.5-3 times in the antifungal compost treated group. The amount of microbes increased markedly in soil supplemented with antifungal compost compared to the control. In the field experiment for cultivation of garlic and Perilla japonica, the growth of both crops was significantly enhanced in the field treated with antifungal compost as compared to the commercial compost.
Studies of the Effect of Vinegar Ingestion after the Strenuous Wingate Test on Energy Substrates during Recovery Periods
Song, Young-Ju ; Ryu, Sung-Pil ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1345~1352
DOI : 10.5352/JLS.2010.20.9.1345
We investigated the effect of brown-rice vinegar (BRV) ingestion after strenuous Wingate tests on energy substrates during a 2 hr recovery period. For this, seven healthy male adolescents were chosen as subjects. They performed 3 Wingate tests to induce fatigue, after which they ingested brown-rice vinegar (BRV) drink and/or water as a control (CON) after 15 min of the test. Blood was obtained pre-exercise and 30 min, 60 min, and 120 min post-exercise, and blood glucose, lactate, free fatty acids, ammonia, and cortisol were analyzed. After 120 min of recovery period, the 4th Wingate test was conducted to calculate the power recovery ratio of the 3rd and 4th trials. Breathing frequency, oxygen saturation, and heart rate did not show significant changes. Blood glucose level was lower in CON than BRV after 120 min of recovery, and blood lactate and ammonia levels were lower in BRV at 60 and 120 min. The higher free fatty acids were found at 60 and 120 min in BRV. In addition, Wingate power recovery ratio of peak power and peak power/body weight was significantly higher in BRV compared to CON. These results suggested that BRV ingestion after strenuous exercise facilitates fatigue recovery. Therefore, BRV might be effectively used as an ergogenic aid for events in which competitors compete two or more times a day.
A Study on the Soil Respiration in Cutting and Uncutting Areas of Larix leptolepis Plantation
Lee, Kyu-Jin ; Mun, Hyeong-Tae ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1353~1357
DOI : 10.5352/JLS.2010.20.9.1353
Quantification of the ecosystem respiration is essential in understanding the carbon cycling of natural and disturbed landscapes. Soil respiration and some environmental factors which affect soil respiration were investigated in a Larix leptolepis plantation inKongju, Korea. Soil respiration was measured at midday of the
day of every month from May to December in a non-cutting area (Control) and a cutting area (Treatment) with IRGA Soil Respiration Analyzer. Throughout the study period, average soil temperature and water content were
for control, and
for treatment, respectively. There was a positive correlation (
=0.8905) between soil respiration and soil temperature in the study area. However, there was no significant correlation between soil respiration and soil moisture (
=0.4437). The seasonal soil respiration increased in the summer and decreased in the winter. In August, maximum soil respirations in the control and treatment areas were
, respectively. Total amounts of
evolution in the control and treatment areas from May to December in 2008 were 2,419.2 and 3,610.8
, respectively. The amount of soil respiration in the treatment area was 49.3% greater than in the control. Increased soil respiration in the treatment area may be due to increased soil temperature, which drives increased microbial decomposition. According to our present investigation, forest cutting will increase the atmospheric
by increasing soil respiration.
Effect of Citrus Byproduct on Quality and Fatty Acid Composition of Chicken Eggs
Moon, Yoon-Hee ; Jung, In-Chul ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1358~1364
DOI : 10.5352/JLS.2010.20.9.1358
In this study, the effects of feeding citrus by-products on the quality and fatty acid compositions of chicken eggs were investigated. Two samples of chicken eggs were used for this study: T0 (eggs of chickens that were not fed citrus byproducts) and T1 (eggs of chickens that were fed 4% citrus byproducts). There was no statistically meaningful difference between T0 and T1 in terms of various properties, such as chemical composition, cholesterol content, minerals, vitamins, pigments, and total structural amino acid. The caloric content of the egg yolk was higher in T1 than in T0, and total free amino acid of egg albumin and egg yolk was higher in T1 than in T0 (p<0.05). There was a not significant difference in SFA:UFA ratio between T0 and T1, but the linolenic acid content was higher in T1 than in T0 (p<0.05). The results of this study show that citrus by-products can be used for feed for laying hens by adding them to general feed.
Effects of Extracts from Dried Yam on Antioxidant and Growth of Human Cancer Cell Lines
Jang, Joo-Ri ; Hwang, Seong-Yeon ; Lim, Sun-Young ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1365~1372
DOI : 10.5352/JLS.2010.20.9.1365
We investigated the inhibitory effects of solvent extracts from dried yam on
-induced oxidative stress and growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells). Yam (Dioscoreacea) has been recognized as a healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, and anti-mutagenic activities, as well as its ability to decrease blood glucose and cholesterol levels. In order to determine the protective effect on
-induced oxidative stress, DCFH-DA (dichlorodihydrofluorescin diacetate) assay was conducted. Acetone with methylene chloride (A+M) extract of dried yam appeared to reduce the levels of intracellular reactive oxygen species (ROS) with dose responses. Among the fractions, 85% aq. methanol fraction showed the highest protective effect on production of lipid peroxides. Inhibitory effects of A+M and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner. The treatments of n-hexane, 85% aq. methanol and n-butanol fractions (
mg/ml concentrations) significantly inhibited the growth of both cancer cells (p<0.05). From these results, 85% aq. methanol fraction showed inhibitory effects on cellular oxidation and growth of human cancer cells, suggesting that this fraction may contain active compounds of dried yam.
Plant Growth Promotion of Calystegia soldanella and Ischaemum anthephoroides by the Strain Penicillium citrinum KACC43900
You, Young-Hyun ; Hwang, Jeong-Sook ; Yoon, Hyeok-Jun ; Khan, Sumera Afzal ; Rim, Soon-Ok ; Bae, Jeong-Jin ; Choo, Yeon-Sik ; Lee, In-Jung ; Kong, Won-Sik ; Lee, Byung-Moo ; Kim, Jong-Guk ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1373~1377
DOI : 10.5352/JLS.2010.20.9.1373
Coastal sand-dune plants can survive very effectively with the help of various microbes, especially ecto- and endomycorrihizae. Penicillium citrinum KACC43900 was screened according to growth promoting activity on sand-dune plants Calystegia soldanella and Ischaemum anthephoroides. In this study, coastal sand-dune plants were treated with a fungal culture filtrate of endophytic fungus P. citrinum KACC43900 to confirm the promotion of plant growth. C. soldanella and I. anthephoroides were used as representative coastal sand-dune plants, and their shoot length, plant length, and dry weight were analyzed. The shoot lengths of control C. soldanella and I. anthephoroides were 8.60 cm and 8.12 cm, and those of samples treated with fungal culture filtrates of C. soldanella and I. anthephoroides for 20 days were 16.30 cm and 10.56 cm, respectively. The plant lengths of control C. soldanella and I. anthephoroides were 14.90 cm and 14.80 cm, and those of samples treated with fungal culture filtrates of C. soldanella and I. anthephoroides for 20 days were 24.06 cm and 17.06 cm, respectively. The dry weight of C. soldanella and I. anthephoroides treated with fungal culture filtrates of C. soldanella and I. anthephoroides for 20 days were 0.163 g and 0.032 g, respectively. It was shown that the growth of shoots in C. soldanella and I. anthephoroides was promoted, 89.54% and 29.60%, by culture filtrate of P. citrinum KACC43900.
Heavy Metal Contents in Tissues of Fishes in Andong and Imha Reservoirs
Shin, Myung-Ja ; Park, Young-Mi ; Lee, Jong-Eun ; Seo, Eul-Won ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1378~1384
DOI : 10.5352/JLS.2010.20.9.1378
The present study aimed to investigate the effect of heavy metal contamination in the aquatic environment on the fishes (Opsariichthys bidens, Cyprinus carpio, Hemiculter eigenmanni and Culter brevicauda) inhabiting Andong and Imha reservoirs by comparing and analyzing the heavy metal contents accumulated in their tissues. Heavy metal contents were shown to be higher in the Andong reservoir than in the Imha reservoir. Accumulated heavy metals, Cr, Cu and Pb, were detected in tissues and bone tissues, but Cd was also found in the kidneys. Zn content was the highest among accumulated metals, whereas Cu content was the lowest in all tissues from the fishes in the Andong and Imha reservoirs. However, the amount of Cr in the tissues showed a twofold higher level in the Andong reservoir than that in Imha reservoir. The heavy metal contents of fish inhabiting the Andong reservoir were detected to be higher than those in the Imha reservoir. We also proposed that heavy metal contamination in water and its inhabiting fish is attributed to various heavy metals derived from water and sediments in the water environment of the Andong reservoir.
Ingredients Analysis and Biological Activity of Fermented Angelica gigas Nakai by Mold
Cha, Jae-Young ; Kim, Hyun-Woo ; Heo, Jin-Sun ; Ahn, Hee-Young ; Eom, Kyung-Eun ; Heo, Su-Jin ; Cho, Young-Su ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1385~1393
DOI : 10.5352/JLS.2010.20.9.1385
Three mold strains, Aspergillus oryzae (AOFAG), Aspergillus kawachii (AKFAG), and Monascus purpureus (MPFAG) were used for fermentation of Angelica gigas Nakai powder. The contents of polyphenolic compounds, flavonoids, minerals, decursin and decursinol angelate and the activities of DPPH (
-picrylhydrazyl) free radical scavenging, reducing power, and tyrosinase were measured. The highest contents of phenolic compound and flavonoid were NFAG at 2.78% and MPFAG at 1.18%, respectively. Major minerals were K, Mg, Fe, Na and Ca. Decursin and decursinol angelate were the major ingredients of Angelica gigas according to HPLC analysis. Decursin area was higher in all fermented Angelica gigas than in NFAG. The activities of free radical scavenging and tyrosinase were stronger in all fermented Angelica gigas than NFAG. However, the Fe/Cu reducing powers were stronger in NFAG than all fermented Angelica gigas. Overall, these results may provide the basic data needed to understand the biological activities and chemical characteristics of Angelica gigas fermented by mold for the development of functional foods.
The Effects of Aerobic Training Intensity Difference on Cardiovascular Disease Pathophysiological Pathways Linking Oxidative Tissue Damage in Obese Children
Woo, Jin-Hee ; Shin, Ki-Ok ; Kim, Keun-Soo ; Kim, Young-Il ; Kang, Sung-Hwun ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1394~1401
DOI : 10.5352/JLS.2010.20.9.1394
The purpose of this study was to examine the effect of 12 wk of aerobic exercise on lipid profiles, antioxidant enzyme activities, oxidative products, and autonomic nervous activity (ANA) in children with obesity. We studied 16 children with obesity and 19 age-matched normal weight controls over a period of 12 wk, during which time moderate intense running exercise was performed. Measurements included peak oxygen uptake, body composition, blood lipid profiles, ox-LDL, 8-OHdG, SOD, GPx activities, total mRNA, and ANA. There were no differences in body weight between periods in the OW group, but body weight increased after 12 wk in OR and CO groups. There were no differences in WHR between periods in the OR and CO groups, but the WHR values decreased after 12 weeks in the OW group. In the obese group, the baseline TG was higher than in the controls (p<0.05), while the ANA level was lower. There were differences in antioxidant enzyme gene expressions between periods in all groups. In conclusion, oxidative damage and antioxidant enzyme activities in obese children were found to be similar to those of normal weight children. However, TG was higher and ANA was lower in obese children than in normal weight children. These results indicated that increased TG and decreased ANA levels begins in childhood in obese patients. Also, regular aerobic exercise may modify the antioxidant enzyme gene expression in early life.
Enhancement of Anticarcinogenic Potentials of Submerged-Liquid Culture of Agaricus blazei Murill on Mouse Ascites Cancer by Rice Hull
Kim, Young-S. ; Jang, Wook-J. ; Rakib, A. ; Kwon, Jung-M. ; Ahn, Chae-R. ; Kim, So-Y. ; Cho, Yong-U. ; Ha, Young-K. ; Kim, Jeong-O. ; Ha, Yeong-L. ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1402~1408
DOI : 10.5352/JLS.2010.20.9.1402
The effects of rice hull (RH) powder on the anticarcinogenic activity of submerged-liquid cultures of Agaricus blazei Murill (AB) were assessed for mouse ascites cancers induced by mouse Sarcoma S-180 (S-180) cancer cells. Optimal growth of AB mycelia in the basal liquid culture medium, containing soybean meal, was achieved by culturing at
for 5 days, when evaluated by
-glucan content, Brix, and mycelial weight, relative to other culture conditions. Hot-water extract (HWE) of the submergedliquid culture of AB mycelia grown at
for 5 days exhibited a stronger anticarcinogenic activity, relative to HWE from other culture conditions. No such effects were obtained from AB mycelial cultures by alternative temperature-controlling cultures. Both cytotoxicity for S-180 cells and anticarcinogenic potentials for mouse ascites cancer of the HWE from AB mycelia grown in the basal medium containing 1% RH powder for 5 days at
were significantly (p<0.05) enhanced, relative to HWE from the AB mycelia culture of the basal medium without RH powder. These results indicate that HWE of submerged-liquid culture of AB mycelia, incubated in media containing 1% RH powder at
for 5 days, enhanced anticarcinogenic activity against S-180 cell-induced mouse ascites cancer, and suggest that RH powder is an excellent ingredient for the improvement of the anticarcinogenic potentials of the submerged-liquid culture of mushroom mycelia.
Effects of 8-week Exercise on Bcl-2, Bax, Caspase-8, Caspase-3 and HSP70 in Mouse Gastrocnemius Muscle
Kim, Ki-Bum ; Kim, Yong-An ; Park, Jung-Jun ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1409~1414
DOI : 10.5352/JLS.2010.20.9.1409
The aim of this study was to investigate the effects of exercise on intrinsic and extrinsic apoptosis signaling pathways in skeletal muscle. ICR-type white male mice were divided into a control group (CON: n=10) and an exercise training group (EX: n=10) after a 1 week adaptation period. EX performed treadmill running at 16.4 m/min with a 4% incline, 40 min/day and 5 days/week for 8 weeks. Cervical dislocation was performed at 48 hours after the last bout of exercise, after which gastrocnemius skeletal muscles were immediately collected. The results of verifying the intrinsic apoptosis pathway showed that there were no significant differences in Bcl-2, Bax, or the ratio of Bax/Bcl-2 proteins between EX and CON. On the other hand, the results of verifying the extrinsic apoptosis pathway showed that caspase-8 proteins were significantly lower in EX than in CON (p<0.05). Apoptosis suppressing protein HSP70 was higher in EX than in CON. In addition, caspase-3, which is the final factor for apoptosis, was not activated. These results indicate that apoptosis did not develop since caspase-3 is non-cleaved by the effects of caspase-8 and HSP70 extrinsic pathways rather than Bcl-2 and Bax intrinsic pathways among signal pathways for apoptosis.
Comparative Analysis of Local Green Tea in Korea by STS-RFLP
Cho, Kiu-Hyung ; Jo, Aruna ; Tsuge, Tomohiko ; Kim, Jong-Cheol ; Kim, Ru-Mi ; Yoon, Ho-Sung ; Kim, Gyung-Tae ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1415~1419
DOI : 10.5352/JLS.2010.20.9.1415
Consumption of green tea has increased along with increasing concern regarding healthier lifestyles, and many brands of green tea are sold with a label indicating the region of Korea in which the tea was produced. However, there is little information on identifying the difference between the green tea cultivars according to the region they were grown. Here, 9 green tea cultivars collected from Hadong region, Bosung region, China and Japan were subjected to the STS-RFLP analysis. Using the coding and noncoding DNA regions of genes related to the phenylpropanoid pathway, such as phenylalanine ammonia-lyase, chalcone synthase and dihydroflavonol 4-reductase, we have identified the differences between green tea cultivars according to the region they were grown in. In this study, we showed a STS-RFLP method of green tea analysis which easily distinguished different kinds of tea using the primers as described. In addition, we identified that the green tea cultivars from Hadong and Bosung displayed a different profile when PAL intron was digested with Dde I, suggesting that a rapid authentication system for green tea cultivars grown in different regions in Korea is available.
Roles of Mannose-Binding Lectin on Innate Immunity and Disease
Jang, Ho-Jung ; Park, Jeong-Hae ; Chung, Kyung-Tae ;
Journal of Life Science, volume 20, issue 9, 2010, Pages 1420~1425
DOI : 10.5352/JLS.2010.20.9.1420
Innate immunity is the first line of host defense consisting of various molecules against infectious challenges. Mannose-binding lectin (MBL) belongs to the collectin protein family which takes part of innate immunity and is able to recognize specific carbohydrates on the surface of a variety of infectious agents acting as a pattern recognition molecule. In this way, MBL differentiates self from non-self and interacts with other molecules of the immune system. MBL genotype shows various MBL2 polymorphisms which are responsible for MBL deficiency in a substantial portion of the entire human population and for susceptibility to infectious disease. Therefore, it has been highlighted in the relationship between genetic variants and clinical significance. Here we focus on presenting anoverview of our understanding of MBL structure and functions.