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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 21, Issue 12 - Dec 2011
Volume 21, Issue 11 - Nov 2011
Volume 21, Issue 10 - Oct 2011
Volume 21, Issue 9 - Sep 2011
Volume 21, Issue 8 - Aug 2011
Volume 21, Issue 7 - Jul 2011
Volume 21, Issue 6 - Jun 2011
Volume 21, Issue 5 - May 2011
Volume 21, Issue 4 - Apr 2011
Volume 21, Issue 3 - Mar 2011
Volume 21, Issue 2 - Feb 2011
Volume 21, Issue 1 - Jan 2011
Selecting the target year
Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)
Chae, Chi-Won ; Dutt, Manjul ; Yun, Su-Hyun ; Park, Jae-Ho ; Lee, Dong-Hoon ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1659~1665
DOI : 10.5352/JLS.2011.21.12.1659
In Citrus, an
segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu
C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the
population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 (
=2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding
progenies in Citrus.
Isolation and Characterization of mas1
of Schizosaccharomyces pombe, a Homologue of Human CIP29/Hcc-1 Involved in the Regulation of Cell Division
Cha, Jae-Young ; Shin, Sang-Min ; Ha, Se-Eun ; Lee, Jung-Sup ; Park, Jong-Kun ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1666~1677
DOI : 10.5352/JLS.2011.21.12.1666
The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel gene, the
sociated protein) gene, a homolog of human CIP29/Hcc1, was isolated and characterized from fission yeast Schizosaccharomyces pombe (S. pombe) using a gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 245 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that a PCB (
ox) is located in the promoter region, which controls M-
specific transcription in S. pombe. The quantitative analysis of the
showed that the pattern of expression is similar to that of the septation index. Cytokinesis of mas1 mutant was greatly delayed at
, and a large number of multi-septate cells were produced. The mas1 mutant had 2C, 4C and 6C DNA contents, as determined by FACS analysis. In addition, the number of multi-septate cells significantly increased. When cells were cultured in nitrogen starvation medium to increase proliferation, the abnormal phenotypes of mas1 mutant dramatically increased. These phenotypes could be rescued by an overexpression of the
gene. The mas1 protein localized in the nuclei of S. pombe and human HeLa cells, as evidenced by Mas1-EGFP signals. The abnormal growth pattern and the morphology of mas1 mutant were complemented by a plasmid carrying human CIP29/Hcc-1cDNA. In addition, CIP29 /Hcc-1 transcript level increased in active cell proliferation stages in the developing mouse embryos. These results indicate that the
ishomologous to the human CIP29/Hcc1 gene and is involved in cytokinesis and cell shape control.
Activation of Pro-Apoptotic Multidomain Bcl-2 Family Member Bak and Mitochondria-Dependent Caspase Cascade are Involved in p-Coumaric Acid-Induced Apoptosis in Human Jurkat T Cells
Lee, Je-Won ; Kim, Young-Ho ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1678~1688
DOI : 10.5352/JLS.2011.21.12.1678
The apoptogenic effect of p-coumaric acid, a phenolic acid found in various edible plants, on human acute leukemia Jurkat T cells was investigated. Exposure of Jurkat T cells to p-coumaric acid (50-
) caused cytotoxicity and TdT-mediated dUTP nick-end labeling (TUNEL)-positive apoptotic DNA fragmentation along with Bak activation,
loss, activation of caspase-9, -3, -7, and -8, and PARP degradation in a dose-dependent manner. However,these apoptotic events were completely abrogated in Jurkat T cells overexpressing Bcl-2.Under these conditions, necrosis was not accompanied. Pretreatment of the cells with the pan-caspase inhibitor (z-VAD-fmk) could prevent p-coumaric acid-induced sub-
peak representing apoptotic cells, whereas it failed to block
loss, indicating that the activation of caspase cascade was prerequisite for p-coumaric acid-induced apoptosis as a downstream event of
loss. FADD- and caspase-8-positive wild-type Jurkat T cell clone A3, FADD-deficient Jurkat T cell clone I2.1, and caspase-8-deficient Jurkat T cell clone I9.2 exhibited similar susceptibilities to the cytotoxicity of p-coumaric acid, excluding an involvement of Fas/FasL system in triggering the apoptosis. The apoptogenic activity of p-coumaric acid is more potent in malignant Jurkat T cells than in normal human peripheral T cells. Together, these results demonstrated that p-coumaric acid-induced apoptogenic activity in Jurkat T cellswas mediated by Bak activation,
loss, and subsequent activation of multiple caspases such as caspase-9, -3, -7, and-8, and PARP degradation, which could be regulated by anti-apoptotic protein Bcl-2.
The Anti-Inflammatory Effects of Persicaria thunbergii Extracts on Lipopolysaccharide-Stimulated RAW264.7 Cells
Kim, Sang-Bo ; Seong, Yeong-Ae ; Jang, Hee-Jae ; Kim, Gun-Do ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1689~1697
DOI : 10.5352/JLS.2011.21.12.1689
In this study, we investigated the anti-inflammation effect of Persicaria thunbergii (P. thunbergii) on RAW 264.7 murine macrophage cells. The anti-inflammatory activity of P. thunbergii was determined by measuring expression of the LPS-induced inflammatory proteins, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor-
), and the production of nitric oxide (NO) and prostaglandin
). Methanol extract of P. thunbergii decreased the expression of iNOS, COX-2 and NF-
, and increased the expression of HO-1 in LPS-stimulated RAW264.7 cells. Methanol extract was fractioned by n-butanol, hexane and ethyl acetate (EtOAc) and each fraction was tested for inhibitory effects on inflammation. Among the sequential solvent fractions, the EtOAc soluble fraction was investigated by the expression of prostaglandin
), and showed decreasing form to the dose-dependent manner. EtOAc extract showed the most effective inhibitory activity of the expression of iNOS, COX-2 and NF-
, and the production of NO. The study showed that P. thunbergii has anti-inflammatory activity through the decrease of NO and inhibition of iNOS, COX-2,
expression, and by the increase of HO-1 enzyme. This study needs for more investigation to find out the most effective single compound with anti-inflammatory activity.
Effect of Dendranthema indicum Extracts on Cell and DNA Damage Induced by Oxidative Stress
Park, Young-Mi ; Kim, Jee-In ; Lee, Chang-Ho ; Lim, Jae-Hwan ; Seo, Eul-Won ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1698~1704
DOI : 10.5352/JLS.2011.21.12.1698
In this study, we evaluated the protective effects of ethanol extracts from Dendranthema indicum on cell and DNA damages induced by oxidative stress. Antioxidant activities of D. indicum extracts are higher than scavenging activities of DPPH free radical and hydroxyl radical by 92.8% and 73.8%, respectively, and higher than ferrous iron chelating effects by 59.4%. D. indicum extracts showed a protective effect on oxidative cell damage by inhibiting lipid peroxidation by 90.3% in the control group, and inhibiting expression level of p21 protein by 79.6% for the control group. This means D. indicum extracts have a great protective effect against oxidative stress. DNA fragmentation inhibition in D. indicum extracts were 89.6% for the control group, which makes the movement of DNA tail reduced, and phosphorylation of H2AX was 20.2% of the radical experiment group. This means that D. indicum extracts effectively inhibit DNA fragmentation and H2AX phosphorylation. Taken together, we suggest that ethanol extract from D. indicum has a role as a useful chemopreventor against oxidative damage.
Effect of the Dietary Supplementation of Fermented Spent Mushroom (Pleurotus eryngii) Substrates on the Growth Performance and Carcass Characteristics in Hanwoo Steers
Gal, Sang-Wan ; Moon, Yea-Hwang ; Cho, Soo-Jeong ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1705~1709
DOI : 10.5352/JLS.2011.21.12.1705
This study was carried out to investigate the feeding effects of the dietary supplementation of fermented spent mushroom (Pleurotus eryngii) substrates with Bacillus amyloliquefaciens CS47 and Saccharomyces cerevisiae (F-SMS) on growth performance and carcass characteristics of Hanwoo steers. Thirty two Hanwoo steers were allocated into two feeding groups and assigned equally to two dietary treatments; Control (commercial formula feed for Hanwoo steers and rice straw) and TMR including 30% F-SMS. The nutritional values of TMR including 30% F-SMS was higher crude protein (11.67%) and TDN (72.11%) than rice straw, but not significantly different from commercial formula feed (p<0.05). Feed intake was significantly greater in the TMR including 30% F-SMS than the control (p<0.05), but body weight gain and carcass grades were not influenced by the experimental diets. Based on this study, fermented spent mushroom (Pleurotus eryngii) substrate with Bacillus amyloliquefaciens CS47 and Saccharomyces cerevisiae is may be used as an ingredient of feed in TMR for Hanwoo steer.
Reduction Effect of Microorganisms by Nano Plasma ion (NPi)
Kang, Hyeon-Cheol ; Yun, Han-Seong ; Sung, Bong-Jo ; Lee, Sung-Hwa ; Lee, Jang-Woo ; Seo, Yong-Bae ; Lee, Myung-Suk ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1710~1715
DOI : 10.5352/JLS.2011.21.12.1710
The bactericidal effect of nano plasma ion (NPi) which was generated by NPi was analyzed using different kinds of microorganisms, exposure times, chamber sizes, ion amounts and distance. As the result of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella pneumoniae, Staphylococcus aureus and Bacillus subtilis were shown different in decrement. Gram-negative bacteria E. coli showed the highest percentage (96.57%) and Gram-positive bacteria B. subtilis which produced spore has the lowest percentage (57.41%). From the exposure time of NPi most of the microorganisms were extinct at an early stage. According to the size of the chamber we compared the loss of E.coli and the experiment result shown, analyzed NPi using 5 chambers
for 2 hr, that when volume of the chamber increased, saturation ion and bactericidal effect was decreased. In addition, an NPi generator installed in the
chamber investigated the decrement of E. coli. Saturation ion concentration increased with decrement. Finally, E. coli showed a similar reduction according to the distance from NPi generator.
Isolation of Bacillus subtilis CK-2 Hydrolysing Various Organic Materials
Kim, Chul-Ho ; Lee, Sang-Hyup ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1716~1720
DOI : 10.5352/JLS.2011.21.12.1716
A bacterium hydrolysing various organic materials including cellulose, protein, starch and lipid was isolated. The isolate was identified as Bacillus subtilis, and named Bacillus subtilis CK-2 in this paper. This bacterium showed optimal growth at
, pH 6~9, and 0~3% of NaCl. B. subtilis CK-2 seemed to synthesis highly active autolysin. The hydrolytic enzymes produced by B. subtilis CK-2 were primary enzymes because extracellular enzyme activities varied similarly to the growth curve. The hydrolytic enzymes seemed to be stable at basic pH conditions. From these results, B. subtilis CK-2 was found to bea useful bacterial agent for composting, or for use in feed-production waste in agriculture, fishery, forest materials, livestock farming, and food.
Anti-Allergic Effects of Nodakenin in IgE/Ag-Induced Type I Hypersensitivity
Kim, Yong-Jae ; Park, Sae-Jin ; Kim, Tack-Joong ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1721~1725
DOI : 10.5352/JLS.2011.21.12.1721
Mast cells are major effector cells associated with allergic responses. They are activated through the release of histamine, arachidonic acid, and proinflammatory cytokines. We investigated the effect of nodakenin, derived from the roots of Angelica gigas Nakai, on mast cell degranulation and on an allergic response in an animal model. We also investigated the effect of nodakenin on expression of multiple cytokines. Nodakenin suppressed the release of
-hexosaminidase, a marker of degranulation, as well as the expression of interleukin IL-4 and TNF-
mRNA. Nodakenin inhibited the passive cutaneous anaphylaxis (PCA) reaction in ICR mice in a dose-dependent manner. These results suggest that nodakenin can inhibit mast cell degranulation through the inhibition of IL-4 and TNF-
mRNA expression, and that nodakenin may potentially serve as an anti-allergic agent.
Construction of Transgenic Silkworms Expressing Human Stem Cell Factor (hSCF)
Kim, Sung-Wan ; Yun, Eun-Young ; Kim, Seong-Ryul ; Park, Seung-Won ; Kang, Seok-Woo ; Kwon, O-Yu ; Goo, Tae-Won ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1726~1731
DOI : 10.5352/JLS.2011.21.12.1726
Human Stem Cell Factor (hSCF) is a cytokine that binds to the c-Kit receptor and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. To produce the human Stem Cell Factor (hSCF) recombinant protein, we constructed a germline transgenic silkworm using the piggyback vector. The expression of the hSCF gene was driven by the Drosophila heat shock protein 70 (dHsp70) promoter. 3XP3 promotor-driven EGFP was used as a marker which allowed us to rapidly distinguish the transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,020 eggs of bivoltin silkworms, Keomokjam. We obtained approximately 22 G1 broods that were EGFP-positive. The expression of the hSCF gene in the transgenic silkworm was analyzed by SDS-PAGE and immunoblotting. Also, analysis of insertion sites into the silkworm genome using inverse PCR showed that exogenous DNA was inserted into the transgenic silkworm genome. These results show that successfully constructed transgenic silkworm expresses the hSCF recombinant protein.
Quality Change of Pork Patty Containing Lotus (Nelumbo nucifera) Leaf and Root Powder during Refrigerated Storage
Park, Kyung-Sook ; Park, Hyun-Suk ; Choi, Young-Joon ; Moon, Yoon-Hee ; Lee, Kyung-Soo ; Kim, Min-Ju ; Jung, In-Chul ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1732~1739
DOI : 10.5352/JLS.2011.21.12.1732
This study was carried out to investigate the effect of lotus root and leaf powder on the quality characteristics of pork patty stored at
for 9 days. The pork patties were of four types: nothing added(control, T0), 0.5% lotus root powder added (T1), 0.25% lotus root and 0.25% lotus leaf powder added (T2), and 0.5% lotus leaf powder added (T3). The
value tended to decrease with longer storage period (p<0.05). The
value of T3 had the lowest value among the samples, the
value T2 and T3 were higher than those of T0 and T1 (p<0.05). Water holding capacity decreased with longer storage period (p<0.05), the water holding capacity, cooking loss, increase rate of thickness and decrease rate of diameter were not significantly different among the samples. Hardness and chewiness increased and springiness decreased with longer storage period (p<0.05). The pH creased with longer storage period (p<0.05), but the VBN content not changed during storage. The TBARS values increased with longer storage period (p<0.05), and those of T0, T1, T2 and T3 were 4.57, 1.85, 0.43 and 0.41 mg MA/kg, respectively, after 9 days of the storage. The result suggest that the addition of lotus root and leaf powder at the same time, or addition of lotus leaf powder can be applied to pork patty to its functionality.
Comparison of Volatile Flavor Compounds in Commercial Black Onion Extracts
Jeon, Seon-Young ; Jeong, Eun-Jeong ; Baek, Jeong-Hwa ; Cha, Yong-Jun ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1740~1745
DOI : 10.5352/JLS.2011.21.12.1740
Volatile flavor compounds of 3 commercial products of black onion extract (produced in Changnyeong, Muan and Jeungpyeong) purchased in the online/offline markets were analyzed. A total of 51 compounds were detected in samples by solid phase microextraction (SPME)/GC/MSD, consisting mainly of carbonyl compounds (15), sulfur-containing compounds (8), aromatic compounds (6), furans (6), nitrogen-containing compounds (3), alcohols (2), acids (2) and miscellaneous compounds (10). Among carbonyls, 4 compounds including 2-methylbutanal, 3-methylbutanal, nonanal and benzaldehyde were detected in all samples, while two sulfur containing compounds, dimethyl disulfide and dimethyl trisulfide, were detected in high amounts and considered to be key flavors in black onion extracts. Particularly, thiophenes and 3 sulfur containing compounds (methylpropyl disulfide, methyl-(Z)-propenyl disulfide and methyl-(E)-propenyl disulfide) were detected only in two products. With acetic acid, furfural and pyrazines that formed through Maillard reaction during black onion aging were detected in high amounts in all samples. This also contributed to characteristic flavors such as roasted, sweet, and sour, in the flavor of black onion extracts.
Inhibitory Effects of High Concentrations of Estrogen, Progesterone and Tamoxifen on Proliferation of HeLa in Culture
Min, Gye-Sik ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1746~1751
DOI : 10.5352/JLS.2011.21.12.1746
This study examined the effects of estrogen, progesterone and tamoxifen at different concentrations and treatment periods on proliferation of a human cervical carcinoma cell line, HeLa, in culture, based on MTT assay. Estrogen did not have an effect on the cellular proliferation in concentrations up to
/ml for treatment periods of between 2.5 and 6 days, but significantly inhibited proliferation at a higher concentration of
/ml in a progressive manner with increasing treatment periods. Also, treatment of HeLa with more than
/ml of progesterone for 2.5 days significantly inhibited proliferation and caused a concentration-dependent inhibition with 4 days of treatment. However, longer treatment with progesterone for 6 days abolished the concentration-dependent inhibitory effect on cellular proliferation observed with the 4-day treatment period. Furthermore, tamoxifen required a higher concentration (
/ml) than estrogen to bring about the inhibitory effect on the HeLa proliferation. These results suggest that high concentrations of estrogen, progesterone and tamoxifen may suppress proliferation of HeLa, and both the concentration and the treatment period may also influence their inhibitory effects on cellular proliferation.
ROS Scavenging Effect and Cell Viability of Opuntia humifusa Extract on Osteoblastic MC3T3-E1 Cells
Hwang, Hyun-Jung ; Jung, Bok-Mi ; Kim, Mi-Hyang ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1752~1760
DOI : 10.5352/JLS.2011.21.12.1752
In this study, the effect of the Opuntiahumifusa extracts on proliferation, alkaline phosphatase (ALP) activity, collagen synthesis and ROS level of a cell was investigated using an osteoblast. Opuntiahumifusawas separated intoOpuntiahumifusapeel (OH-P), seed (OH-Se) and stem (OH-St).These were subjected to extraction by using hot water and ethanol. The proliferation of the MC3T3-E1 osteoblastic cells that were treated with OH-Se water extract were increased by approximately 120%. Regarding the effects of OH-Se on ALP activity, the
ethanol extract group showed the highest activity. The synthesis of collagen increased significantly in response to treatment with OH-Se water extract. The ROS scavenging effects of Opuntiahumifusawere investigated for involvement of oxidativedamage, cell culture and staining. Also, when OH-Se water extract
was added, the ROS level decreased by 54%. These results indicate that Opuntiahumifusa extracts have an anabolic effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases.
Inhibitory Effects of a New Herbal Composition (HemoHIM) on UVB-Induced Suppression of Langerhans Cell's Accessory Cell Function
Kim, Jong-Jin ; Jo, Sung-Kee ; Jung, U-Hee ; Park, Hae-Ran ; Yee, Sung-Tae ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1761~1771
DOI : 10.5352/JLS.2011.21.12.1761
In the previous results, we developed an effective products to apply as functional foods for overcome of radiation damage and reduction of side effects in radiotherapy. To verify the prevention of UVB-induced immunosuppression of immune cell function by HemoHIM, we studied on the mechanism of the skin immune function for the protection in UVB irradiation. In studies presented here, we showed that HemoHIM can prevent UVB-induced impairment of skin immune cell function by in vitro and in vivo assay. Exposure of freshly cultured murine dendritic cells (DCs) with IL-4/GM-CSF to UVB irradiation resulted in impairment of accessory function. This suppression could be prevented by addition of HemoHIM before or after to the cultures of UVB-irradiated DCs. We also tested the effects of HemoHIM on the suppression of contact hypersensitivity (CHS) treated oral or intraperitoneal administration. This UVB-suppressed CHS was prevented by administration of HemoHIM to UVB-irradiated mice. These results suggest that HemoHIM may prevent UVB-induced immune suppression in the skin.
Expression of Anion Exchanger and CFTR in the Hepatocyte and Cholangiocytes in Bile Duct-Ligated Rat
Lee, Jae-Dong ; Wang, Joon-Ho ; Ki, Seung-Seog ; Choe, Won-Hyeok ; Park, Jae-Seung ; Cho, Won-Kyoo ; Park, Jung-Jun ; Kim, Hong-Su ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1772~1777
DOI : 10.5352/JLS.2011.21.12.1772
Cystic fibrosis transmembrane conductance regulator (CFTR) gene and sodium-independent
anion exchanger (AE) genes are expressed in a wide variety of mammalian tissues including cholangiocytes. They play an important role in the regulation of intracellular pH (pHi) as well as in transepithelial acid/base transport necessary for biliary bicarbonate secretion. The aim of this study was to examine the expression level of CFTR gene and AE genes (AE1, AE2 and AE3) in the cholangiocytes and the hepatocytes, and also measure AE2 gene expression level after bile duct ligation (BDL). As we previously described, isolated hepatocytes and cholangiocytes from the liver of normal and BDL rats were prepared and gene expression levels were measured by using RT-PCR. We found that AE1, AE2, and AE3 genes were expressed in both hepatocytes and cholangiocytes, but CFTR was only in cholangiocytes. AE2 gene expression level was higher in the BDL hepatocytes than normal hepatocytes, which was significantly different between two groups. AE2 gene expression level was lower in the BDL cholangiocytes than normal cholangiocytes. However, AE2 gene expression level in both hepatocytes and cholangiocytes were not changed with a longer duration of BDL. These results suggest that CFTR and AE2 may play an important role in the pathogenetic mechanism of biliary cholestatic liver disease.
Modulation of G-CSF Secretion by Mutations of Non Alpha-Helical Region in N-Terminus
Park, Jeong-Hae ; Park, Jung-Ae ; Kang, Seok-Woo ; Goo, Tae-Won ; Chung, Kyung-Tae ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1778~1783
DOI : 10.5352/JLS.2011.21.12.1778
Hematopoietic cytokines regulate production of blood cells by stimulating proliferation and differentiation of bone marrow cells. Among these hematopoietic cytokines, called hematopoitic growth factors, glranulocyte-colony stimulating Factor (G-CSF), which regulates growth of neutrophils, is one of important therapeutic factors because cancer patients suffer with neutropenia which is severe reduction of neutrophils after chemotherapy. Two groups of recombinant G-CSF have approved and used for therapeutic purposes and many researches are still on-going to produce recombinant G-CSF by different techniques. We engineered human G-CSF with Bombyx specific endoplasmic reticulum (ER) signal sequence, therefore, secretion of human G-CSF protein was improved in Bombyx mori-origined cell line, Bm5. The Bombyx ER signal sequence and human G-CSF matured protein region chimera was further remodeled at the N-terminus of matured G-CSF protein to understand roles of N-terminus on outer cellular secretion and/or production. Three different mutants were generated deleting three amino acids in non alpha-helical region in N-terminus in order to scan important amino acids for G-CSF secretion. One of 3 different N-terminal deletion mutants showed dramatically reduction of secreted amount of G-CSF indicating its important role on secretion. The data suggest that remodeling in non alpha-helical region of N-terminus is also important for recombinant G-CSF production.
miR-7b Promoter Contains Negative Gene Elements
Choi, Ji-Woong ; Lee, Heon-Jin ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1784~1788
DOI : 10.5352/JLS.2011.21.12.1784
The typical miRNA and its nearby host gene are co-expressed by sharing the same promoter. We assumed that miR-7b and its host gene FICT might use an identical promoter for their brain specific gene expression. Sequence comparison of the genomic DNA of mouse miR-7b, human miR-7-3 and their host genes by using the bioinformatic tools revealed high sequence homology and several putative transcription factor-binding sites on the promoter region. In order to probe the hypothesis we used a luciferase vector system into which we cloned the 5' upstream conserved region of miR-7b and FICT. The putative promoter region showed decreased luciferase activity, suggesting that the 5' upstream of miR-7b and FICT contain a negative regulator for gene expression.
Sarijang Enhances Maturation of Murine Bone Marrow-Derived Dendritic Cells
Jin, Cheng-Yun ; Han, Min-Ho ; Park, Cheol ; Hwang, Hye-Jin ; Choi, Eun-A ; Choi, Yung-Hyun ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1789~1794
DOI : 10.5352/JLS.2011.21.12.1789
Dendritic cells (DCs) are professional antigen-presenting cells playing key roles in immune sentinels as initiators of T-cell responses against microbial pathogens and tumors. Sarijang, a folk sauce containing extracts of Rhynchosia nulubilis, Ulmus davidiana roots, Allium sativum, and Rhus Verniaiflura bark, has been used as a nonspecific immunostimulant for cancer patients. However, little is known about its immunomodulating effects or their mechanisms. In this study, we investigated whether sarijang induces phenotypic and functional maturation of DCs. For this study, murine bone marrow-derived myeloid DCs were cultured in the presence of interleukin-4 (IL-4) and granulocyte-macrophage colony stimulating factor (GM-CSF), and the generated immature DCs were stimulated with sarijang or lipopolysaccharide (LPS). Our data indicated that sarijang significantly enhanced the expression of co-stimulatory molecules (CD80 and CD86) as well as major histocompatibility complex (MHC) II, as did LPS. The results provide new insight into the immunopharmacology of sarijang and suggest a novel approach to the manipulation of DC for therapeutic application.
Potential Roles of Hedgehog and Estrogen in Regulating the Progression of Fatty Liver Disease
Hyun, Jeong-Eun ; Jung, Young-Mi ;
Journal of Life Science, volume 21, issue 12, 2011, Pages 1795~1803
DOI : 10.5352/JLS.2011.21.12.1795
Non-alcoholic fatty liver disease accompanies the rise in the prevalence of obesity, diabetes and the tendency toward high-fat dietary habits. Specifically, the higher prevalence of non-alcoholic fatty liver disease in men and postmenopausal women seems to be caused by the protective effects of estrogen against liver fibrosis, or lack thereof. There are no effective preventive therapies for liver diseases because the mechanisms underlying the progression of fatty liver diseases to chronic liver diseases and the protective effects of estrogen against fibrogenesis remain unclear. Recently, it has been reported that the hedgehog signaling pathway plays an important role in the progression of chronic liver diseases. Hedgehog, a morphogen regulating embryonic liver development, is expressed in injured livers but not in adult healthy livers. The level of hedgehog expression parallels the stages of liver diseases. Hedgehog induces myofibroblast activation and hepatic progenitor cell proliferation and leads to excessive liver fibrosis, whereas estrogen inhibits the activation of hepatic stellate cells to myofibroblasts and prevents liver fibrosis. Although the mechanism underlying the opposing actions of hedgehog and estrogen on liver fibrosis remain unclear, the suppressive effects of estrogen on the expression of osteopontin, a profibrogenic extracellular matrix protein and cytokine, and the inductive effects of hedgehog on osteopontin transcription suggest that estrogen and hedgehog are associated with liver fibrosis regulation. Therefore, further research on the estrogen-mediated regulatory mechanisms underlying the hedgehog-signaling pathway can identify the mechanism underlying liver fibrogenesis and contribute to developing therapies for preventing the progression of fibrosis to chronic liver diseases.