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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 21, Issue 12 - Dec 2011
Volume 21, Issue 11 - Nov 2011
Volume 21, Issue 10 - Oct 2011
Volume 21, Issue 9 - Sep 2011
Volume 21, Issue 8 - Aug 2011
Volume 21, Issue 7 - Jul 2011
Volume 21, Issue 6 - Jun 2011
Volume 21, Issue 5 - May 2011
Volume 21, Issue 4 - Apr 2011
Volume 21, Issue 3 - Mar 2011
Volume 21, Issue 2 - Feb 2011
Volume 21, Issue 1 - Jan 2011
Selecting the target year
T0901317 as an Inhibitor of Transcriptional Activation of Constitutive Androstane Receptor (CAR)
Kim, Hyun-Ha ; Seol, Won-Gi ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 481~485
DOI : 10.5352/JLS.2011.21.4.481
T0901317 is a potent synthetic ligand for liver X receptor (LXR, NR1H2/3), a member of the nuclear receptor superfamily that functions as a transcription factor. However, T0901317 has been also reported to modulate the activity at least four other nuclear receptors (NRs), acting as agonists for farnesoid X receptor (FXR, NR1H4) and pregnane X receptor (PXR, NR1I2) and as antagonists for androgen receptor (AR, NR3C4) and retinoid-related orphan receptor-
, NR1F1). We report here that T0901317 can also function as an inhibitor for constitutive androstane receptor (CAR, NR1I3). Since CAR is a major player of xenobiotic and cholesterol metabolism in the liver, along with PXR, FXR and LXR, which are reported to be regulated by T0901317, this further complicates the interpretation of potential results with T0901317 in liver cells.
Involvement of Peroxynitrite in NO Donor-Induced HO-1 Expression in Rat Articular Chondrocytes
Song, Ju-Dong ; Kim, Kang-Mi ; Kim, Jong-Min ; Yoo, Young-Hyun ; Park, Young-Chul ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 486~493
DOI : 10.5352/JLS.2011.21.4.486
Nitric oxide (NO) donors are a potent inducer of heme oxygenase-1 (HO-1). However, it is unclear whether or not HO-1 expression induced by NO donors is a direct consequence of NO released by NO donors. Here, we investigated the effects of NO donors on the expression of HO-1 in primary rat articular chondrocytes. NO donors (SIN-1, SNAP, and SNP) significantly induced the accumulation of HO-1 protein accompanied by an increase in HO-1 mRNA. NO donor-induced HO-1 expression exerted cytoprotection against NO and/or superoxide-induced cell death. Guanylate cyclase signaling was not associated with Nrf2 and HO-1 expression in NO donor-treated chondrocytes. Interestingly, NO scavenger carboxy-PTIO and SOD mimetic TEMPOL markedly inhibited NO donor-induced HO-1 expression in chondrocytes. In addition, NO donor-induced HO-1 expression was completely abrogated by the peroxynitrite scavenger MnTBAP. Since peroxynitrite can be physiologcally formed in the cell through reaction of NO with superoxide, we analyzed whether or not peroxynitrite could directly induce HO-1 expression in chondrocytes. Peroxynitrite treatment in chondrocytes evoked doseand time-dependent Nrf2 and HO-1 expression. These results indicate that HO-1 expression induced by NO donors in rat articular chondrocytes is due to NO-mediated peroxynitrite rather than NO.
Coat Color Patterns and Genotypes of Extension and Agouti in Hanwoo and Jeju Black Cattle
Han, Sang-Hyun ; Cho, In-Cheol ; Kim, Jae-Hwan ; Ko, Moon-Suck ; Kim, Young-Hoon ; Kim, Eun-Young ; Park, Se-Pill ; Lee, Sung-Soo ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 494~501
DOI : 10.5352/JLS.2011.21.4.494
To understand the relationship between coat color inheritance patterns and genotypes of Extension (E) and Agouti (A) loci in cattle, the genotypes for melanocortin-1 receptor (MC1R) and agouti signaling protein (ASIP) were analyzed in Hanwoo, Jeju black cattle (JBC), and their crossbred progeny. Three MC1R alleles (
, and e) were found in the black-colored JBC population. JBC had no recessive homozygotes (e/e), but this genotype was predominant in the Hanwoo breed. However, MC1R
/e Hanwoo did not produce a black coat color as they appeared either as brown or solid red. For ASIP, three genotypes (A/A, A/
) were determined by insertion/deletion of an L1-BT element in Hanwoo. The ASIP
allele was rarely observed, and no ASIP
homozygotes were detected in the JBC population. Cattle carrying ASIP
did not show any agouti-like brindle pigmentation patterns in either breed or their progeny. The coat colors of the crossbred progeny were discriminated by two colors, yellowish-brown versus dark-brown or black, and their coat colors were directly related to the genotypes of the Extension locus, yellowish-brown (e/e) and dark-brown or black (
/e), but not to the Agouti locus. ASIP genotypes probably did not affect coat color development in the Hanwoo or crossbred progeny. Our results suggest that the ASIP genotypes do not play key roles in coat color variation, but the MC1R genotypes do direct the phenotypes of Hanwoo, JBC, and their progeny.
Early Identification of Putative Zygotic Seedlings in Citrus Crosses between 'Morita unshiu' (Citrus. unshiu Marc.) and 'Ponkan' (C. reticulata Blanco) Using RAPD and SRAP
Yun, Su-Hyun ; Moon, Young-Sun ; Jin, Seong-Beom ; Kang, In-Kyu ; Lee, Dong-Hoon ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 502~508
DOI : 10.5352/JLS.2011.21.4.502
The purpose of this study was to evaluate the methods of identifying zygotic seedlings of crosses between 'Morita unshiu' (Citrus. unshiu Marc.) and 'Ponkan' (C. reticulata Blanco). In order to investigate the frequency and position of zygotic seedlings and to determine the efficiency of zygotic seedling identification, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) were performed using UBC (9, 27, 229, 230, and 254) primers and F4/R27, F7/R14, F12/R10, and F44/R62 primer sets, respectively. A total of 37 putative zygotic seedlings out of 55 individuals were selected by RAPD and SRAP. The F7/R14 primer pair showed a screening ability of 45.5% (25/55), whereas the primer UBC27 revealed the highest efficiency of zygotic seedling identification (50.9%, 28/55). When both UBC27 and F7/R14 were properly used for selection of hybridized seedlings of 'Morita unshiu' (C. unshiu Marc.) and 'Ponkan' (C. reticulata Blanco), screening efficiency was increased to 60% (33/55) for putative zygotic seedlings. Thus, it is possible to select putative hybrid zygotic seedlings in an accurate and effective manner by RAPD and SRAP.
Quality Characteristics of Kiwi Wine and Optimum Malolactic Fermentation Conditions
Kang, Sang-Dong ; Ko, Yu-Jin ; Kim, Eun-Jung ; Son, Yong-Hwi ; Kim, Jin-Yong ; Seol, Hui-Gyeong ; Kim, Ig-Jo ; Cho, Hyoun-Kook ; Ryu, Chung-Ho ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 509~514
DOI : 10.5352/JLS.2011.21.4.509
Maloactic fermentation (MLF) occurs after completion of alcoholic fermentation and is mediated by lactic acid bacteria (LAB), mainly Oenococcus oeni. Kiwi wine more than commercial grape wine has the problem of high acidity. Therefore, we investigated the optimal MLF conditions for regulating strong acidity and improving the quality properties of wine fermented with Kiwi fruit cultivated in Korea. For alcohol fermentation, industrial wine yeast Saccharomyces cerevisiae KCCM 12650 strains and LAB, known as MLF strains, were used to alleviate wine acidity. First, the various experimental conditions of Kiwi fruit, initial pH (2.5, 3.5, 4.5), fermenting temperature (20, 25,
), and sugar contents (24
), were adjusted, and after the fermentation period, we measured the acidity, pH, and the change in organic acid content by the AOAC method and HPLC analysis. The alcohol content of fermented Kiwi wine was 12.75%. Further, total acidity and pH of Kiwi wine were 0.78% and 3.5, respectively. Total sugar and total polyphenol contents of Kiwi wine were 38.72 mg/ml and 60.18 mg/ml, respectively. With regard to organic acid content, the control contained 0.63 mg/ml of oxalic acid, 2.99 mg/ml of malic acid, and 0.71 mg/ml of lactic acid, whereas MLF wine contained 0.69 mg/ml of oxalic acid, 0.06 mg/ml of malic acid, and 3.12 mg/ml of lactic acid. Kiwi wine had lower malic acid values and total acidity than control after MLF processing. In MLF, the optimum initial pH value and fermentation temperature were 3.5 and
, respectively. Therefore, these studies suggest that establishment of optimal MLF conditions could improve the properties of Kiwi wine manufactured in Korea.
Immunomodulating Effect of Edible Mushrooms in Mice
Park, Hyun-Ji ; Heo, Yong ; Kim, Jong-Bong ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 515~520
DOI : 10.5352/JLS.2011.21.4.515
This study was carried out to evaluate the immunomodulatory capacity of edible mushrooms, including Lepista nuda, Corprinis comatus, Letinus edodes, and Pleurotus eryngii, in mice. BALB/c mice were administered 1, 50, and 500 mg/kg body weight of various mushrooms five times a week over 4 weeks through gastric intubation. The control mice were administered distilled water. No significant changes in body weight were observed. IL-4 and IFN
production was evaluated with splenic T lymphocytes stimulated in vitro with phytohemagglutinins for 48 hr. The mice group administered L. edodes showed significantly higher ratio of IFN
versus IL-4 than the other groups. In addition, the ratio of plasma IgG2a versus IgG1 was also significantly elevated in mice treated with L. edodes. However, no significant change was observed in ratio of IgG2a versus IgG1 in splenic B lymphocytes stimulated in vitro with lipopolysaccharides for 7 days. These results indicate that L. edodes can enhance type-1 helper T cell-mediated cellular immunity.
Effects of Hatha Yoga Exercise on Body Composition, Serum Lipids, and Health-Related Fitness of Obese Middle-Aged Women
Kim, Do-Yeon ; Lee, Jeong-Ah ; Yang, Jum-Hong ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 521~528
DOI : 10.5352/JLS.2011.21.4.521
The purpose of this study was to analyze the effects of Hatha Yoga exercise on the body composition, serum lipids, and health-related fitness of obese middle-aged women. The subjects in the Hatha Yoga exercise group (n=9) were 18 obese middle-aged women that trained for 16 weeks and a control group (n=9). Body composition, serum lipids, and health-related fitness were measured in all of the subjects before the start of the training program and at the end of the 16 weeks. The findings of this study were as follows. Body weight, %BF, BMI, WHR, VFA, TC, TG, LDL-C, FFA, and HOMA-IR significantly decreased, whereas LBM, HDL-C, grip strength, back strength, push-ups, sit-ups, Harvard step test, and sitting trunk flexion significantly increased in the Hatha Yoga exercise group after 16 weeks. Therefore, regular and continuous Yoga exercise was effective in improving body composition, serum lipids, and health-related fitness. Consequently, Yoga exercise could be effective for preventing cardiovascular disease caused by obesity in middle-aged women.
Induction of Metallothionein Gene by Laminin in Normal and Malignant Human Prostate Epithelial Cells
Ock, Mee-Sun ; Cha, Hee-Jae ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 529~533
DOI : 10.5352/JLS.2011.21.4.529
Metallothioneins (MT) are a group of low-molecular weight, cysteine-rich, intracellular proteins that are encoded by a family of genes containing at least 10 functional isoforms in human. The expression and induction of these proteins is associated with protection against DNA damage, oxidative stress, and apoptosis. Many studies have shown increased expression of MT in various human tumors, whereas MT is down-regulated in certain tumors such as hepatocellular carcinoma and liver adenocarcinoma. Hence, the expression of MT is not universal to all human tumors but may depend on the differentiation status and proliferative index of tumors, along with other tissue factors and gene mutations. Using Northern blot analysis, we found that laminin induced expression of MT-1 in HSG and PC12 cells, which can be differentiated by laminin, but had no effect on MB-231, MDA-435, and PC-3 cells, which cannot be differentiated by laminin. In addition, we analyzed the expression level of the MT-1 gene in five prostate cancer cell lines possessing different metastatic potential. The expression of MT-1 in normal and less malignant cells (RWPE-1 and WPE1-NA22) was high and up-regulated by laminin, whereas the expression of MT-1 in WPE1-NB14, WPE1-NB11, and WPE1-NB26 cells (malignant) was extremely low and not elevated by laminin. These results suggest that the MT-1 gene is involved in laminin-mediated differentiation and affects the metastatic potential of tumor cells.
Retinoic Acid Potentiates Nitric Oxide-Induced Dedifferentiation through the ERK Pathway in Rabbit Articular Chondrocytes
Yu, Seon-Mi ; Kim, Song-Ja ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 534~541
DOI : 10.5352/JLS.2011.21.4.534
Retinoic acid (RA), a metabolite of vitamin A, is known to regulate dedifferentiation of rabbit articular chondrocytes. The regulatory mechanism of dedifferentiation by RA is not yet understood. Thus, the effect of RA on the regulation of nitric oxide (NO)-induced dedifferentiation was investigated in rabbit articular chondrocytes. RA caused loss of the differentiated chondrocyte phenotype as demonstrated by inhibition of type II collagen expression and proteoglycan synthesis. RA also accelerated NO-induced dedifferentiation in rabbit articular chondrocytes as detected by expression of type II collagen and Sox-9 using Western blot analysis and production of sulfated proteoglycan using Alcain blue staining. Further, RA potentiated NO-induced activation of ERK. Inhibition of ERK with PD98059 (PD) recovered the expression of type II collagen and Sox-9 and production of sulfate proteoglycan in NO-induced dedifferentiated chondrocytes by RA treatment. Our findings suggest that RA accelerates NO-induced dedifferentiation of rabbit articular chondrocytes via the ERK pathway.
Effects of Different Exercise Intensity on FDEIA and Related Mechanisms
Lee, Won-Jun ; Kwak, Yi-Sub ; Yoo, Byung-In ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 542~548
DOI : 10.5352/JLS.2011.21.4.542
Food-dependent exercise-induced anaphylaxis (FDEIA) is a distinct form of food allergy induced by physical exercise. It is typified by the onset of anaphylaxis during exercise, which is preceded by the ingestion of causal food allergens. Diagnosis of FDEIA is heavily dependent on clinical history. To describe the physiopathological mechanism, etiologic factors, and clinical manifestations, we evaluated the spleen index, proliferation assay of lymphocyte, ROS, ASAS, and cytokines levels in sensitized and exercise-trained mice. One-hundred mice were bred in the animal lab at D and P university under controlled conditions [
, RH 45-55%, and a 12-hour photoperiod]. Animals are 7-weeks-old at the time of study and were fed a standard commercial chow diet from 09:00 to 15:00 over the 8-week study period. The mice were allowed access to distilled deionized water ad libitum. Daily food intake and weekly body gains were routinely recorded throughout the experimental period using computing scale (CAS). Mice were divided into the control group (S; control sensitized, n=25), 30 min swim training group (S30, N=25), 50 min swim training group (S50, N=25), and 80 min swim training group (S80, N=25). The results were as follows: Spleen index showed the highest level in the S80 group compared to other groups; this level was exercise-dependent. In proliferation assay of Med and OVA, the S80 group showed the highest level compared to the other groups; this level also was exercise intensity- dependent. Peritoneal ROS and IL-4 showed a statistically significant difference compared to S; however, there was no significant differences in ROS among S30, 50, and 80. From the results, we concluded that FDEIA is correlated with exercise intensity based on the levels of peritoneal ROS and cytokine profiles.
Novel Method for Urinary 1-Hydroxypyrene Measurement Using Molecular Imprinting
Yim, Dong-Hyuk ; Moon, Sun-In ; Choi, Young-Sook ; Park, Hee-Jin ; Kim, Dae-Seon ; Yu, Seung-Do ; Lee, Chul-Ho ; Kim, Yong-Dae ; Kim, Heon ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 549~553
DOI : 10.5352/JLS.2011.21.4.549
This study was performed to determine whether or not urinary 1-hydroxypyrene (1-OHP) levels can be accurately detected by our 1-OHP-detecting
-Bead-HPLC assay that we developed based on the molecular imprinting method. Our method showed a variation coefficient of 4.97% and a between-day variation coefficient of 4.43%, suggesting that this may be a very stable method. In addition, the recovery rate of 1-OHP from a mixture of 1-OHP and similar substances using our
-Bead-HPLC method was estimated to be 105.6%. The correlation coefficient between the conventional enzyme-HPLC method and this new method was 0.74 (p<0.01) when the urine samples were tested. Based on this result, it is conceivable that our method could be a useful technique for measuring urinary 1-OHP levels. Moreover, our method has some advantages of being easier and less expensive than the conventional method. The results of this study suggest that our method can facilitate the development of a urine 1-OHP sensor using
-coating beads and that development of beads by molecular imprinting can be applied to analysis of chemicals other than 1-OHP.
Isolation of Microorganisms and Development of Microbial Augmentation for Treatment of Paper Mill Wastewater
Kang, Dae-Ook ; Suh, Hyun-Hyo ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 554~560
DOI : 10.5352/JLS.2011.21.4.554
This study was performed to investigate the effects of microbial augmentation on the biological treatment of paper mill wastewater. Three bacteria (KN11, KN13, KN27) capable of degrading aromatic compounds and a bacterial strain (GT21) producing an extracellular cellulase were isolated from soil and wastewater by selective enrichment culture. Through morphological, physiological, and biochemical taxonomies, isolated strains of KN11, KN13, KN27, and GT21 were identified as Acinetobacter sp., Neisseria sp., Bacillus sp., and Pseudomonas sp. and named Acinetobacter sp. KN11, Neisseria sp. KN13, Bacillus sp. KN27, and Pseudomonas sp. GT21, respectively. For analysis of non-biodegradable and chemical oxygen demand (COD)-increasing matter in a paper mill wastewater, we utilized GC/MS to detect aromatic compounds and their derivatives containing several substituted functional groups. The microbial augmentation, J30 formulated with the mixture of bacteria including Acinetobacter sp. KN11, Neisseria sp. KN13, Bacillus sp. KN27, and Pseudomonas sp. GT21, was used for the treatment of paper mill wastewater. The optimum temperature and pH for COD removal of the microbial augmentation, J30, were
and 7.5, respectively. For evaluation of the industrial applicability of the microbial augmentation, J30 in the pilot test, treatment efficiency was examined using paper mill wastewater. The microbial augmentation, J30, showed a COD removal rate of 87%. On the basis of the above results, we designed the wastewater treatment process of the activated sludge system.
Anti-Inflammatory and Anti-Oxidative Activity of Methanol Extract from Terminalia chebula Retz., Lavandula spica L., and Dalbergia odorifera T. in RAW 264.7 Cells
Chae, In-Gyeong ; Yu, Mi-Hee ; Kim, Hyuk-Il ; Lee, In-Seon ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 561~567
DOI : 10.5352/JLS.2011.21.4.561
This study was performed to evaluate the anti-inflammatory and antioxidant activities of methanol extract from natural products. Cell viability was determined by MTT assay. The production of NO and TNF-
were measured by Griess assay and enzyme-linked immunosorbent assay (ELISA). In order to effectively screen for anti-inflammatory agents, we first examined the inhibitory effects of 24 natural products on the production of lipopolysaccharide (LPS)-induced nitric oxide (NO) in RAW 264.7 cells. Three extracts of Terminalia chebula Retz., Lavandula spica L., and Dalbergia odorifera T. significantly inhibited NO production. The three extracts significantly decreased production of NO in a dose-dependent manner. Terminalia chebula Retz. decreased TNF-
production. Antioxidative effects of the three extracts were measured based on polyphenol and flavonoid contents and DPPH radical scavenging activity assay. The three extracts showed high polyphenol contents as well as strong DPPH scavenging activities. In particular, Terminalia chebula Retz. contained the highest polyphenol and flavonoid levels of 616 and
, respectively, compared to Lavandula spica L. and Dalbergia odorifera T. As DPPH radical scavensing activities, RC50 values of Terminalia chebula Retz. were
Effect of Feeding Mugwort (Artemisia capillaris) TMR Fodder on Nutritional Composition of Hanwoo Beef
Moon, Yoon-Hee ; Yang, Seung-Ju ; Jung, In-Chul ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 568~575
DOI : 10.5352/JLS.2011.21.4.568
TMR feed developed by adding mugwort originating from Jeju Island,was fed to Hanwoo to investigate the effects of feeding mugwort on nutritional composition, as well as the feasibility of producing Hanwoo beef with high quality and functionality. The samples for the experiment consisted of Hanwoo fed fattening cattle TMR feed without mugwort (control), and Hanwoo fed fattening cattle TMR feed with added mugwort (treatment). Feeding mugwort did not lead to a significant difference in the moisture, crude protein, crude fat, crude ash, calorie, cholesterol, and collagen contents. There was no significant difference between the control and treatment groups in mineral components. The control and treatment groups had total mineral amounts of 3,559.20 and 3,998.01 ppm, respectively. The total amount of structural amino acids did not show any significant difference between the control (14.56%) and treatment groups (13.90%). The total amount of free amino acids did not show any significant difference between the control (763.044 ppm) and treatment groups (720.885 ppm). The composition of saturated fatty acids was 42.956% for the control and 40.918% for the treatment group. Further, the composition of unsaturated fatty acids was 57.044% for the control and 59.082% for the treatment group. Myristic acid, myristoleic acid, pentadecenoic acid, palmitic acid, and
-linolenic acid were increased by feeding mugwort. Arachidic acid was contained the most among the fatty acid of Hanwoo fed feed without mugwort (p<0.05).
Evaluation of Antioxidant and Nitrite Scavenging Activity of Seaweed Extracts
Ahn, Seon-Mi ; Hong, Yong-Ki ; Kwon, Gi-Seok ; Sohn, Ho-Yong ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 576~583
DOI : 10.5352/JLS.2011.21.4.576
In the course of study for the development of functional food ingredients from seaweeds having useful biological activities, the in-vitro antioxidant and nitrite scavenging activities of the methanol extracts prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta) were determined. At
concentration of the methanol extracts, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis (Kjellman) Setchell, Ishige foliacea, I. okamurai, Sargassum confusum, S. fulvellum, S. yamade and Zostera marina showed 60% more DPPH and ABTS scavenging activity. The exceptions were found in I. okamurai and Z. marina, which showed 45% and 53% ABTS scavenging activity, respectively. The correlation coefficient between DPPH and ABTS scavenging activities was 0.855, suggesting that the 9 different seaweeds extracts could scavenge anion and cation radicals concurrently. In reducing power, only E.cava, E.stolonifera and E.bicyclis showed above 0.88 (
). In a while, in nitrite scavenging activity at
concentration of the methanol extracts, 19 different seaweeds extracts including I. foliacea, I. okamurai, S. confusum, S. fulvellum, and S. yamade showed 60% more activity. Calculation of
of DPPH, ABTS and nitrite scavenging activities of 9 different seaweeds extracts further showed that I. foliacea and S. yamade, besides E.cava, E.stolonifera and E.bicyclis, have strong antioxidant and nitrite scavenging activity. These results suggest that the selected 9 different seaweeds could be developed as functional food ingredients and I. foliacea and S. yamade have potential as novel natural sources of antioxidant and nitrite scavenger.
Effects of Organic Materials, Chitosan, Wood Vinegar, and EM Active Solution on Soil Microbial and Growth in Chinese Cabbage
Jeong, Soon-Jae ; Cho, Mi-Yong ; Seok, Woon-Young ; Lee, Sang-Lok ; Lee, Hyoung-Joo ; Oh, Ju-Sung ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 584~588
DOI : 10.5352/JLS.2011.21.4.584
This study was conducted to investigate the effects of organic materials, chitosan, wood vinegar, and EM active solution, on soil microbial and growth in Chinese cabbage. The organic materials were treated with chitosan, wood vinegar, and EM (effective microoganism) active solution, and the treatment concentration was 100 times solution and 1,000 times solution level with foliar application. The results are summarized as follows: Among foliar application of organic materials treatment plot[?], with 1,000 times solution level of chitosan was effective in inhibiting microbial growth in Chinese cabbage compared to other plots and control. The microorganism number in the soil for cultivation of Chinese cabbage increased with organic materials treatment plot as compared with control. Especially, 1,000 times solution level of chitosan showed the most significant effect.
In vitro Antimicrobial Activity of a New Isolate Streptomyces sp. BCNU 1030
Bang, Ji-Hun ; Choi, Hye-Jung ; Ahn, Cheol-Soo ; Kim, Dong-Wan ; Jeong, Yong-Kee ; Joo, Woo-Hong ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 589~595
DOI : 10.5352/JLS.2011.21.4.589
This work focused on screening and characterizing antibiotic-producing actinomycetes to develop new antibiotics that can overcome the growing resistance of disease-causing microbes. One-hundred actinomycetes strains were isolated from soil samples from Chungcheongbuk-do, Korea using various kinds of actinomycetes isolation media, including a starch casein agar medium and potato dextrose agar (PDA). Among them, strain BCNU 1030 was determined to show strong antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). Biochemical, physiological, and 16S rRNA sequence analyses indicated that strain BCNU 1030 belonged to the genus Streptomyces. Strain BCNU 1030 exhibited antibiotic activity against a wide range of bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of BCNU 1030 dichloromethane extract was determined to be
for MRSA CCARM 3090. Therefore, Streptomyces sp. BCNU 1030 has potential for anti-MRSA drug development.
Antioxidant and Anti-Inflammatory Activities of Extracts of Traditional Medicinal Plants Mixtures 1 and 2
Cho, Eun-Kyung ; Choi, Young-Ju ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 596~603
DOI : 10.5352/JLS.2011.21.4.596
This study was conducted to investigate traditional medicinal plants (TMP) 1 and 2, two different multi-herbal mixtures consisting of 24 herbs. Regarding the contents of flavonoid compounds, the ethanol extract (EE) of TMP2 yielded the highest content of flavonoid compounds (40.981 mg/g), followed by EE of TMP1 (28.23 mg/g), hot water extract of TMP2 (WE, 10.046 mg/g), and WE of TMP1 (6.59 mg/g). Antioxidant activities of EE and WE of TMP1 and TMP2 were measured based on DPPH radical scavenging activity. At 1 mg/ml, the highest DPPH radical scavenging activity was shown in EE of TMP2 (96%), followed by EE of TMP1 (94%). Nitric oxide (NO) production by RAW264.7 macrophage cells stimulated with lipopolysaccharide (LPS) was reduced to 82, 77, 86, and 47% by addition of
of EE and WE of TMP1 and TMP2, respectively. These results were not due to the cytotoxicity of the extracts. NO synthesis was increased by 2.3, 3.6, 3.0, and 1.8 fold by addition of
of EE and WE of TMP1 and TMP2, respectively. These results show that medicinal plants play a significant role in antioxidative activity and activation of the immune system in the pathogenesis of inflammatory diseases, and different mixtures of the medicinal plants showed different effects.
Effect of Pine (Pinus densiflora) Needle Hot Water Extract on Antioxidant Activity in Rats Treated with Carbon Tetrachloride
Park, Yong-Soo ; Park, Mi-Ra ; Jeon, Min-Hee ; Hwang, Hyun-Jung ; Kang, Min-Suk ; Kim, Bo-Kyung ; Kim, Sung-Gu ; Lee, Sang-Hyeon ; Kim, Mi-Hyang ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 604~609
DOI : 10.5352/JLS.2011.21.4.604
Pine (pinus densiflora) needles have long been used as a traditional health-promoting medicinal food in Korea. This study was conducted to investigate the effects of pine needle extracts on the hepatic antioxidant system in the damaged liver of carbon tetrachloride (
)-treated rats. Nine-week-old Sprague Dawley rats were divided into four groups: normal group (NOR),
-treated group (CCL), pine needle hot water extract and
-treated group (CCL-P), and Vitamin C and
-treated group (CCL-V). The enzyme activities and antioxidant effects of the pine needle hot water extracts were investigated at the levels of liver homogenates and serum of rats intoxicated with
. Serum GOT and GPT activities by
treatment increased compared to those of the NOR group. However, they tended to decrease in the hot water extract-administered group. Liver SOD activity in the CCL group was significantly lower than the NOR group (p<0.05). However, they increased in the CCL-P group compared to the CCL group. Further, the CAT and GPx activities of serum treated with
were higher compared to those of the NOR group but lower in the CCL-P group compared to CCL group. These results suggest that pine needle hot water extract increases antioxidant activities.
Spectrophotometric Assay for Determination of Chlorogenic Acid Using Green Pigment Formation and Quantitative Analysis of Chlorogenic Acid in Blueberry Leaf
Chung, Dong-Min ; Chung, Young-Chul ; Chun, Hyo-Kon ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 610~612
DOI : 10.5352/JLS.2011.21.4.610
We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at
under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-
). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.
Characterization of ERp29 and ADP-Ribosylation Factor 5 Interaction
Kwon, Ki-Sang ; Seog, Dae-Hyun ; Kim, Seung-Whan ; Yu, Kweon ; Kwon, O-Yu ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 613~615
DOI : 10.5352/JLS.2011.21.4.613
ERp29 is a endoplasmic reticulum (ER) lumenal resident protein that shows sequence similarity to the protein disulfide isomerase family. Its biological function is thought to play a role in the processing of secretory proteins within the ER, possibly by participating in the folding of proteins in the ER. Although some data on ERp29 have been reported, its normal functions are still unclear. To gain insights into the function of ERp29, we identified ARF5 protein as a protein that interacts with ERp29 using yeast two-hybrid screening and GST pull-down assay. Interaction between ERp29 and ARF5 was detected under normal cell conditions but not under ER stress conditions. This result may provide a clue for understanding ERp29 biological functions.
Histone H3K27 Modifications and Gene Transcription
Kim, Ae-Ri ;
Journal of Life Science, volume 21, issue 4, 2011, Pages 616~620
DOI : 10.5352/JLS.2011.21.4.616
Lysine residues of histone H3 and H4 are covalently modified in the chromatin of eukaryotic cells. Lysine 27 in histone H3 was acetylated (H3K27ac) or methylated at three levels; mono-, di-, and trimethylation (H3K27me1, H3K27me2, and H3K27me3). These modifications at H3K27 were related with gene transcription and/or chromatin structure in distinct patterns. Generally, H3K27ac and H3K27me1 were enriched in active chromatin, such as the locus control region or transcriptionally active genes, while transcriptionally inactive genes were highly marked by H3K27me2 and H3K27me3. These modifications appear to have been catalyzed by distinct histone-modifying enzymes. Recent studies suggest that the four kinds of modifications at H3K27 have inter-correlation in gene transcription or chromatin structure formation.