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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 21, Issue 12 - Dec 2011
Volume 21, Issue 11 - Nov 2011
Volume 21, Issue 10 - Oct 2011
Volume 21, Issue 9 - Sep 2011
Volume 21, Issue 8 - Aug 2011
Volume 21, Issue 7 - Jul 2011
Volume 21, Issue 6 - Jun 2011
Volume 21, Issue 5 - May 2011
Volume 21, Issue 4 - Apr 2011
Volume 21, Issue 3 - Mar 2011
Volume 21, Issue 2 - Feb 2011
Volume 21, Issue 1 - Jan 2011
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Inhibition of Cell Invasion by Indole-3-Carbinol in OVCAR-3 Human Ovarian Cancer Cells
Choi, Yung-Hyun ; Kim, Sung-Ok ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 923~931
DOI : 10.5352/JLS.2011.21.7.923
In the present study, we investigated the effect of indole-3-carbinol (I3C), a natural compound present in vegetables, on the cell migration and invasion of OVCAR-3 ovarian cancer cells. Our results indicated that I3C inhibited the proliferation of OVCAR-3 cells, a process which was associated with inhibition of cell motility as determined by wound healing experiments and cell invasion studies. I3C treatment increased the tightness of the tight junctions (TJs), which was demonstrated by an increase in transepithelial electrical resistance and a decrease in paracellular permeability. The RT-PCR and immunoblotting results indicated that I3C repressed the levels of claudin-3 as well as claudin-4, proteins that comprise a major part of TJs and play a key role in the control and selectivity of paracellular transport. Furthermore, the activities of matrix metalloproteinase (MMP)-2 and MMP-9 were also decreased by treatment with I3C, which was connected with the down-regulation of their mRNAs and protein expression. The results suggest that I3C may be expected to inhibit cancer cell metastasis and invasion by restoring TJs and decreasing MMP activity in ovarian cancer cell line OVCAR-3.
Antihyperlipidemic Effect of Ginsenoside Rg1 in Type 2 Diabetic Mice
Park, Jae-Hong ; Lee, Ji-Youn ; Yeo, Ji-Young ; Nam, Jeong-Su ; Jung, Myeong-Ho ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 932~938
DOI : 10.5352/JLS.2011.21.7.932
Ginsenoside Rg1 is a pharmacologically active component isolated from ginseng. The goal of this study was to clarify the beneficial effects of Rg1 on glucose and lipid metabolism in diabetic animals (db/db mice). To accomplish this, ten week old db/db mice were administered 10 mg/kg of Rg1 for 15 days. Rg1 did not influence the weight of db/db mice when compared with vehicle-treated db/db mice. The administration of Rg1 lowered fasting plasma glucose, and improved glucose tolerance. Importantly, Rg1 markedly reduced both plasma triglyceride and free fatty acids, and increased high-density lipoprotein cholesterol (HDL-C) concentrations in db/db mice. Rg1 activated promoter activity of chimeric GAL4-PPAR
reporter and increased expression of peroxisome proliferator-activated receptor alpha (PPAR
) target genes such as carnitine palmitoyltransferase-1 (CPT-1) and acyl-CoA oxidase (ACO), which are involved in fatty acid oxidation. These findings indicated that improvement of lipid profiles by Rg1 may be associated with increased fatty acid oxidation via PPAR
activation. Taken together, these results suggest that Rg1 could have beneficial effects for controlling hyperglycemia and hyperlipidemia associated with type 2 diabetes.
Identification and Phylogenetic Relationship at Cytochrome Oxidase Subunit I (COI) Gene among Korean Terrestrial Planarian Taxa
Moon, Doo-Ho ; Lee, Young-Ah ; Huh, Man-Kyu ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 939~946
DOI : 10.5352/JLS.2011.21.7.939
Sequence data of Cytochrome Oxidase Subunit I (COI) gene of mitochondria were used to elucidate the taxonomy and phylogenetic relationships of the terrestrial planarian taxa in Korea. Published COI gene sequences from Family Bipaliidae in GenBank were also included in the phylogenetic analysis. The aligned data sets for Terricola ranged from 387 to 444 nucleotides (bp) as a result of differences in insert nucleotides. The phylogeny based on COI analysis was not congruenced with the morphological traits. Bipalium nobile included the remainder taxa (Bipalium adventitium, Bipalium venosum, Bipalium kewense, and Bipalium multilineatum). Internal nodes were strongly supported (>91%). The phylogenetic tree on COI analysis showed that most identified species were well separated from each other. The main phylogenetic analysis formed monophyletic groups. COI gene of mitochondria could have the resolving power for taxonomy information for the terrestrial planarian taxa in Korea.
Detection and Typing of Human Papillomavirus in Cutaneous Common Warts by Multiplex Polymerase Chain Reaction
Choi, Soon-Yong ; Lim, Jong-Ho ; Kim, Eun-Jung ; Kim, Hei-Sung ; Kim, Beom-Joon ; Kang, Hoon ; Park, Young-Min ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 947~952
DOI : 10.5352/JLS.2011.21.7.947
A number of epidemiological studies have identified human papillomavirus (HPV) types 1, 2, 3, 4, 7, 10, 27, 57, and 65 in cutaneous common warts. However, identification of the HPV subtype by conventional polymerase chain reaction (PCR) is time consuming with its multi-step laboratory process. In this study, we aim to develop a specific one-step multiplex polymerase chain reaction method which capably identifies six different HPV genotypes related to common warts. By HPV DNA sequence analysis, 6 pairs of specific primers were designed from the intergenic regions of genes L1 to E6, and from genes E2 to L2. DNA sequence analysis with the L1 gene sequence of the sample was performed to measure the specificity of multiplex PCR. HPV-1, -2, -3, -4, -27, and -57 were identified without cross amplification in 109 out of 129 samples. The sensitivity and specificity of our set of primers in detecting HPV were 85% and 99.5%, respectively. For the 20 samples where HPV type was not identifiable by our batch of primer sets, multiplex PCR with an additional set of HPV primers was done, where 7 were found positive for HPV-7 or -65. Our results demonstrate that the newly designed multiplex PCR can rapidly detect the specific HPV subtype involved in common warts with high accuracy.
HMGB1 Switches Alkylating DNA Damage-Induced Apoptosis to Necrosis
Lee, Su-Yeon ; Jeong, Eui-Kyong ; Jeon, Hyun-Min ; Ju, Min-Kyung ; Kim, Cho-Hee ; Park, Hye-Gyeong ; Kang, Ho-Sung ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 953~960
DOI : 10.5352/JLS.2011.21.7.953
Necrosis is characterized by the cell membrane rupture and release of the cellular contents, including high-mobility group box 1 protein (HMGB1), into the extracellular microenvironment. HMGB1 acts as a transcriptional regulator in nuclei, but exerts a pro-inflammatory and tumor-promoting cytokine activity when released into the extracellular space. Its overexpression is associated with tumor progression and chemoresistance. Thus, HMGB1 acts as a clinically important molecule in tumor biology. In this study, we examined whether HMGB1 affects cell death induced by anti-cancer drugs. Here we show that HMGB1 prevented cisplatin (alkylating agent)-induced apoptosis and switched the cell fate to necrosis in MCF-7, MDA-MB231, and MDA-MB361 cells. Similar apoptosis-to-necrosis switch effects of HMGB1 were observed in cells treated with 4-HC, another alkylating agent. In contrast, HMGB1 did not exert any significant effects on docetaxel (DOC)-induced apoptosis in MCF-7 cells. We also show that cisplatin-induced apoptosis was switched to necrosis in MCF-7 multicellular tumor spheroids (MTS) that were cultured for 8 days and had necrotic cores, but DOC-induced apoptosis was prevented without the apoptosis-to-necrosis switch. Finally, the levels of RAGE, a receptor of HMGB1, were increased with extended culture of MTS. These findings demonstrate that HMGB1 switches alkylating agent-induced apoptosis to necrosis, suggesting that the strategy to prevent necrosis occurring as an undesirable action of alkylating agent-based chemotherapy should be delineated to improve the efficacy of chemotherapy for cancer.
4-Hydroxynonenal Induces Endothelial Cell Apoptosis via ROS and Peroxynitrite Generation
Chung, Sang-Woon ; Yee, Su-Bog ; Lee, Ji-Young ; Hossain, Mohammad Akbar ; Kim, Dong-Hwan ; Yoon, Jeong-Hyun ; Chung, Hae-Young ; Kim, Nam-Deuk ; Kim, Nam-Deuk ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 961~968
DOI : 10.5352/JLS.2011.21.7.961
The formation of reactive lipid aldehydes, 4-hydroxynonenal (HNE) is shown to be derived from fatty acid hydroperoxides through the oxidative process. Among its known effects in cytotoxicity, HNE has been implicated in apoptotic cell death. To delineate its putative role as a potential mediator, we investigated the mechanism by which HNE induces apoptosis of endothelial cells (ECs). The anti-proliferative effects of HNE were tested through MTT assay after exposure to various concentrations (
) of HNE. We observed apoptotic bodies with propidium iodide staining, and measured the HNE induction of endothelial apoptosis by flow cytometry assay. We observed that cells exposed to HNE for 24 hr resulted in increased poly(ADP-ribose) polymerase cleavage and up-regulation of Bax. Data on the HNE action strongly indicated the involvement of reactive species, namely, intracellular ROS, nitrite, and peroxynitrite. To obtain evidence on the implication of ROS and peroxynitrite in HNE-induced apoptosis, a ROS scavenger, N-acetylcysteine (NAC), and a peroxynitrite scavenger, penicillamine, were tested. Results clearly indicate that the induction of apoptosis by HNE was effectively inhibited by NAC and penicillamine. Based on the present data, we conclude that the endothelial apoptosis induced by HNE involves both ROS generation and peroxynitrite activity. Our new data could lead to a redefinition of HNE action on apoptosis in ECs.
The Roles of Phytohormones and AtEXPA3 Gene in Gravitropic Response of Arabidopsis thaliana
Yun, Hye-Sup ; Lee, Yew ; Kim, Seong-Ki ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 969~975
DOI : 10.5352/JLS.2011.21.7.969
We focused on relationship between phytohormones and AtEXPA3 gene in gravitropic response of A. thaliana. RT-PCR analysis shows that AtEXPA3 was highly expressed in actively developing tissues such as leaf, rosette, root and flower tissues. AtEXPA3 gene expression was enhanced by gravistimulation, BR and IAA. Furthermore, decreased gravitropism was observed when treatment of AVG, an ethylene biosynthetic inhibitor, suggesting that ethylene has a gravistimulating effect itself as well as BRs and IAA. Inhibition of gravitropism in AtEXPA3 RNAi mutant suggests that BR, auxin and ethylene are playing roles as regulators of AtEXPA3. In addition, altered gravitropism in BRs signaling mutant (decreased in bri1-301, bak1, and increased BRI-GFP) indicated that BRs signaling mediated the gravitropism. In conclusion, gravitropic responses of Arabidopsis root resulting from root growth were mediated by increased expression of AtEXPA3 gene, which is stimulated by phytohormones.
Application of Isolated Tyrosinase Inhibitory Compounds from Persimmon Leaves
Cho, Young-Je ; An, Bong-Jeun ; Kim, Jeung-Hoan ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 976~984
DOI : 10.5352/JLS.2011.21.7.976
Total phenolic content was the highest in 60% ethanol extracts at 21.91 mg/g, and inhibitory activity against tyrosinase of 60% ethanol extracts was higher than ethanol extracts of other concentration. The inhibitory compounds against tyrosinase from Persimmon leaves were purified using Sephadex LH-20, MCI-gel CHP-20 column chromatography with gradient elution. Two purified compounds were isolated as a result. The chemical structures of each compound were determined and identified using
-NMR, FAB-Mass. The compounds were confirmed as (+)-gallocatechin and prodelphinidin B-3. The tyrosinase inhibitory activities of purified (+)-gallocatechin and prodelphinidin B-3 were 29.5 and 40.2%, respectively. The inhibitory activities of (+)-gallocatechin and prodelphinidin B-3 against melanin biosynthesis in melanoma cell were 32.5 and 46.7%. The safety of essence with tyrosinase inhibitory compounds from persimmon leaves was also assessed by various safety profiles. First, changes in pH (4.90~4.95) and viscosity (23,000~26,000 cP) was not detected for 60 days. Essence also showed stability against temperature and light for 60 days. All these findings suggest that extracts from persimmon leaves have a great potential as a cosmetical ingredient with a potent whitening effect.
The Effects of Treadmill Training on Neurotrophins and Immediately Early Protein in Obese Rats
Woo, Jin-Hee ; Shin, Ki-Ok ; Yeo, Nam-Heoh ; Park, So-Young ; Kang, Sung-Hwun ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 985~991
DOI : 10.5352/JLS.2011.21.7.985
The purpose of this study was to investigate the biological effect of obesity-induced oxidative damage on neurogenesis and early protein expression. Obesity was induced I thirty 4-week old male Sprague-Dawley rats through a high fat diet for 15 weeks. After one week of environmental adaptation, the rats were divided into 2 groups: high fat diet sedentary group (HDS, n=15) and high fat diet training group (HDT, n=15). Exercise training was performed 5 times a week for 8 weeks, with mild-intensity treadmill running for weeks 1-4 and moderate-intensity treadmill running for weeks 5-8. After the 8 week training period, we analyzed lipid profiles, serum 8-hydroxyguanosine (8-OHdG), liver tissue malondialdehyde (MDA) related to oxidative damage factors, nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), c-fos, c-jun, and extracellular signal regulated kinase (Erk) in the hippocampus. The results of this study are as follows. There were differences between HDS and HDT in triglyceride (TG) and total cholesterol (TC) (p<0.05). In high density lipoprotein (HDL-c), the HDT was higher than HDS after treadmill training (p<0.05). In 8-OHdG, the HDT was lower than HDS after treadmill training (p<0.05). Genetic expressions of c-jun, BDNF and MDA in the HDT were higher than in the HDS after treadmill training in hippocampus (p<0.05). Therefore, we conclude that 8 weeks of treadmill training can improve imbalanced lipid profiles, reduce oxidative damage, and activate neurogenesis in obese rats.
Diversity and Plant Growth-Promotion of Endophytic Fungi Isolated from the Roots of Plants in Dokdo Islands
You, Young-Hyun ; Yoon, Hyeok-Jun ; Lee, Gil-Seong ; Woo, Ju-Ri ; Rim, Soon-Ok ; Shin, Jae-Ho ; Lee, In-Jung ; Choo, Yeon-Sik ; Kim, Jong-Guk ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 992~996
DOI : 10.5352/JLS.2011.21.7.992
Endophytic fungi were isolated from the roots of plants growing naturally on the island of Dokdo. Plant samples, such as Miscanthus sinensis, Achyranthus japonica and Echinochloa crusgali were isolated from Dongdo, and those such as Honkenya peploides and Artemsia koidzumii were isolated from Seodo. Twenty one strains of endophytic fungi were isolated from these plants. To identify the strains, PCR (polymerase chain reaction) amplification of the partial ITS (Internal Transcribed Spacer) regions was done with universal primers ITS-1 and ITS-4 to determine the nucleotide sequence of the ITS regions. Of the strains isolated from Miscanthus sinensis, 75% were Penicillium sp. and 25% were Aspergillus sp. Fifty five percent of strains isolated from Achyranthus japonica were Penicillium sp., 30% were Aspergillus sp. and 15% were Zygorhynchus sp. Strains isolated from Echinochloa crusgali were Penicillium sp. (50%), Aspergillus sp. (12%), Giberella sp. (13%), Talaromyces sp. (9%) and Umbelopsis sp. (8%). Of the strains isolated from Honkenya peploides, 76% were Penicillium sp. and 24% were Pestalotiopsis sp. Strains isolated from Artemisia koidzumii were Penicillium sp. (81%) and Mucor sp. (19%). As a result of bioassay, Ec-3-1 strain isolated from Echinochloa crusgalli showed plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp. was the most abundantly distributed fungal strain in all plants used in this study.
Change of Mitochondrial Biogenesis Genes on Regular Exercise Training in Adipocytes of Ovariectomized Rats Fed on High Fat Diet
Lee, Jin ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 997~1003
DOI : 10.5352/JLS.2011.21.7.997
Menopause and obesity are associated with metabolism. The purpose of this study was to examine the changes of PPAR
), NRf-1 and TFAM mRNA and mitochondria biogenesis in adipocytes and investigate the effect of swimming exercise for 6weeks on ovariectomized rats. Rats were randomly assigned to 3 groups: (1) ovariectomized rats fed with a control diet (C, n=4), (2) ovariectomized rats fed with high fat diet (H, n=4), and (3) ovariectomized rats trained to exercise and fed with high fat diet (H+EX, n=4). Exercise was performed by swimming for 5 days/wk, with a progressive increase in exercise over the course of 6 weeks. Results showed that the fat tissue weight in the H group was markedly increased (p<0.01) compared to other groups, however, regular exercise significantly decreased fat weight. The PPAR-
(p<0.05), NRf-1 (p<0.01) and TFAM (p<0.05) mRNA expression in the adipocytes of H+EX were higher than in the H group. These results suggest that regular exercise for 6 weeks might exert positive effects by increasing PPAR-
, PGC-1 (
), NRf-1 and TFAM mRNA expression and mitochondria in adipocytes. Thus, regular exercise may be helpful in the improvement of mitochondria biogenesis function in obese, ovariectomized rats.
Effect of Diniconazole on Growth and Flowering of Vinca rocea and Salvia splendis
Choi, Sung-Hwan ; Kang, Jum-Soon ; Choi, Young-Whan ; Lee, Yong-Jae ; Park, Young-Hoon ; Kim, Mi-Ra ; Son, Beung-Gu ; Kim, Hyeun-Kyeung ; Kim, Hong-Yul ; Oh, Wook ; Sim, Hak-Bo ; Lim, Ki-Byung ; Kim, Jin-Ki ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1004~1008
DOI : 10.5352/JLS.2011.21.7.1004
This study was conducted to investigate the effects of uniconazole (Sumiseven), an existing expensive growth retardant, and diniconazole (Binnari), used as a cheap germicide, on the growth and flowering of Vinca major and Salvia splendis for the possibility of substitution for expensive growth retardants. The growths of V. major and S. splendis were retarded by Binnari treatment. The effect of Binnari 100 mg/l treatment was higher than that of Sumiseven 5 mg/l treatment in both plants. The length of leaves was remarkably retarded by Binnari treatment, however, the width of leaves was only slightly retarded, giving the leaf a round shape. The number of days to flowering was increased by Binnari treatment in both in V. major and S. splendis. The number of days to flowering in V. major showed no significant difference compared to the control except by treatemtn with Binnari 400 mg/l. The number of florets of S. splendis increased with treatment with Binnari 100 and 200 mg/l.
Web-Building Strategy of a Wasp Spider, Argiope bruennichi, under Sensory Information Emitted by a Prey Species
Jin, Woo-Young ; Shin, Hyun-Chul ; Kim, Kil-Won ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1009~1015
DOI : 10.5352/JLS.2011.21.7.1009
Web structure of spiders is a foraging strategy as well as an investment to get prey. In order to increase the fitness consequence, spiders change their foraging strategy based on sensory information provided by prey species. We conducted an experiment to demonstrate whether an orb-web building spider, Argiope bruennichi modifies its web-building behavior after experiencing sensory information emitted by a UV recognizing flying insect, Bombus terrestris. To know how the web structure would be modified, we observed web characteristics after providing sensory information of a potential prey species, the bumblebee (experimental group) and compared them to the control group (no information provided). We hypothesized that the spiders of the experimental group would increase investments in their web construction and stabilimentum decoration. The results rejected our hypothesis. Web and stabilimentum constructions decreased in the course of the experiment in both the control group and the experimental group. The individuals did not increase their stabilimentum length and did not extend their web areas, in spite of sensory information given by the nectar collector.
Biological Activity of Methanolic Extract from Ganoderma lucidum, Momordica charantia, Fagopyrum tataricum, and Their Mixtures
Cha, Jae-Young ; Jin, Jong-Sung ; Cho, Young-Su ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1016~1024
DOI : 10.5352/JLS.2011.21.7.1016
The concentrations of bioactive material (polyphenolics and flavonoids) and the biological activities of antioxidative (DPPH [
-picrylhydrazyl] free radical scavenging activity, peroxidation of rat liver microsome, and Fe/Cu reducing power),
-glucosidase inhibition, tyrosinase inhibition, and fibrinolytic activity were tested by in vitro experimental models using 70% methanolic extract from Ganoderma lucidum, Momordica charantia, Fagopyrum tataricum and their mixtures. The highest yield and the concentrations of polyphenolics and flavonoids were shown in the mixture extract. Mixture extract was shown to have the highest activities of
-glucosidase inhibition and tyrosinase inhibition, fibrinolytic, DPPH free radical scavenging, and Fe/Cu reducing power in a concentration-dependent manner. From these results, mixture methanol extract was shown to have the most potent bioactive properties and to contain large amounts of biological materials such as polyphenolics and flavonoids. This mixture could be a good dietary supplement material candidate, such as for antidiabetic functional foods.
Effects of Aerobic Exercise and Black Garlic Intake on Blood Lipids, Lipid Peroxidation and BAP in Rats
Kim, Seung-Hyun ; Baek, Yeong-Ho ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1025~1031
DOI : 10.5352/JLS.2011.21.7.1025
The purpose of this study was to analyze the effects of aerobic exercise and black garlic intake on blood lipids, lipid peroxidation and BAP in rats. The subjects for the study were 25 male Sprague-Dawley rats composed of the control group (CG, n=6), exercise group (EG, n=6), which trained for 4 weeks (30-60 min/day, speed at 15 m/min), black garlic intake group (BGG, n=7), and exercise with black garlic intake group (EBGG, n=6). Blood lipids, lipid peroxidation, and BAP were measured in all the subjects after the end of the 4 week treadmill exercise period. The findings of this study were as follows; TC and TG were significantly lower in the EBGG compared to other groups, while there were no significant findings for HDL-C and LDL-C levels. TBARS was significantly lower in the EBGG compared to the CG, and the BAP from the EBGG and EG were significantly higher than in the CG and BGG after 4 weeks of treadmill exercise. Therefore, aerobic exercise with black garlic intake was effective in improving TC, TG, TBARS, and BAP. Consequently, aerobic exercise with black garlic intake will be effective for activating antioxidant defenses and decreasing cardiovascular diseases.
Expression and Purification of the Phosphatase-like Domain of a Voltage-Sensing Phosphatase, Ci-VSP
Kim, Sung-Jae ; Kim, Hae-Min ; Choi, Hoon ; Kim, Young-Jun ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1032~1038
DOI : 10.5352/JLS.2011.21.7.1032
Recently identified Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP) consists of an ion channel-like transmembrane domain (VSD) and a phosphatase-like domain. Ci-VSP senses the change of membrane potential by its VSD and works as a phosphoinositide phosphatase by its phosphatase domain. In this study, we present the construction of His-tagged phosphatase-like domain of Ci-VSP, its recombinant expression and purification, and its enzymatic activity behavior in order to examine the biochemical behavior of phosphatase domain of Ci-VSP without interference. We found that Ci-VSP(248-576)-His can be eluted with an elution buffer containing 25 mM NaCl and 100 mM imidazole during His-tag purification. In addition, we found the proper measurement condition for kinetics study of Ci-VSP(248-576)-His against p-nitrophenyl phosphate (pNPP). We measured the kinetic constant of Ci-VSP(248-576)-His at
, pH 5.0 or 5.5, under 30 min of reaction time, and less than
of protein amount. With these conditions, we acquired that Ci-VSP(248-576)-His has
for pNPP dephosphorylation. Therefore, we produced a pure form of Ci-VSP(248-576)-His, and this showed a higher activity against pNPP. This purified protein will provide the road to a structural investigation on an interesting protein, Ci-VSP.
Effects of High-Intensive Exercise Duration on Hematologic Properties and Cytokines in Rats
Hyun, Kyung-Yae ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1039~1045
DOI : 10.5352/JLS.2011.21.7.1039
The effects of duration and time-dependent, high-intensity exercise on hematological properties and inflammation-related parameters in rats were studied. 20, 60, and 120 min of high-intensive exercise were performed daily for 8 weeks. None of the complete blood count (CBC) factors were affected by the exercise, except for the leukocyte concentration which, in the 20 min group, showed an increase of 47% compared to the control, but this was decreased after 60 min by 30% compared to the control. As exercise was performed for 60 min or longer, serum concentrations of
, unsaturated iron biding capacity (UIBC), and total iron biding capacity (TIBC) were significantly elevated in comparison to the control, where 20 min of exercise did not show any change. Both levels of interleukin-6 (IL-6), a pro-inflammatory cytokine, and interleukin-10 (IL-10), an anti-inflammatory cytokine in the serum, were elevated in response to the high-intensity exercise, however the rate of IL-6 increase was higher than the rate of exercise intensity increase, thus the offset of inflammation might be suggested. The concentration of nitric oxide (NO) in the serum became high in response to the exercise. Overall, the current observation suggests that inflammation-like responses to high-intensity exercise might be due to high circulation of blood flow and high oxygen requirement, resulting in systemic damages. However, under the current high-intensity exercise conditions, more than 20 min of exercise might not be suggested for health care purposes.
Effect of Diallyl Disulfide on Heme Oxygenase-1 Expression in Human Hepatoma Cell Line HepG2
Kim, Kang-Mi ; Lee, Sang-Kwon ; Park, Young-Chul ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1046~1051
DOI : 10.5352/JLS.2011.21.7.1046
Diallyl disulfide (DADS), the most prevalent oil-soluble organosulfur compound in garlic, is known to have diverse biological activities, including anticarcinogenic, antiatherosclerotic, antiinflammatory, and antioxidant actions. In this study, we investigated the effect of DADS on the expression of heme oxygenase-1 (HO-1) in human liver hepatoma cell line HepG2. Treatment of HepG2 cells by DADS evoked a dose-dependent growth inhibition without significant toxicity to the cells, and also induced the expression of transcription factor Nrf2. However, DADS did not have any enhancing effect on transcription and translation of HO-1 expression in HepG2 cells. In addition, DADS efficiently blocked protein synthesis of HO-1 in HepG2 cells stimulated by CoPP or hemin. But, DADS did not decrease the content of transcripts of HO-1 gene stimulated by CoPP, with accumulation of Nrf2 and small Maf in the nucleus. Based on these results, we conclude that DADS inhibits HO-1 expression by modulation of translational level of CoPP or hemin-induced HO-1 expression in HepG2 cells.
Antioxidative Activity and Chemical Characteristics of Leaves, Roots, Stems and Fruits Extracts from Acanthopanax senticosus
Heo, Su-Jin ; Ahn, Hee-Young ; Kang, Min-Jeong ; Lee, Jae-Hong ; Cha, Jae-Young ; Cho, Young-Su ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1052~1059
DOI : 10.5352/JLS.2011.21.7.1052
The contents of bioactive and antioxidative activities (DPPH (
-picrylhydrazyl), free radical scavenging activity, peroxidation of linoleic acid and rat hepatocyte microsome, and Fe/Cu reducing power, tyrosinase inhibition activity) were tested by in vitro experimental models using water, hot water, ethanol and methanol extracts of leaves (ASL), roots (ASR), stems (ASS) and fruits (ASF) from Acanthopanax senticosus. Hot water extract from ASL showed the highest extraction yield (16.04%) as well as highest contents of phenolic compounds (2.67%) and flavonoids (1.43%). Major minerals were K, Ca and Mg. In oxidation in vitro models using DPPH free radical scavenging activity, Fe/Cu reducing power,
/ascorbate-induced linoleic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods, tyrosinase inhibition activity and autooxidation of rat hepatic microsomes membrane, and antioxidative activities were strong in Acanthopanax senticosus. From these results, ASL extracts were shown to have the most potent antioxidative properties and contain the highest amounts of antioxidative compounds such as phenolic compounds and flavonoids. These results may provide the basic data to understand the biological activities of bio-active materials derived from leaves of Acanthopanax senticosus.
Ecotypic Variation Related to the Ratio of Mannose to Galactose In the Seeds of Phaseolus angularis
Kim, Chang-Ho ;
Journal of Life Science, volume 21, issue 7, 2011, Pages 1060~1066
DOI : 10.5352/JLS.2011.21.7.1060
In order to investigate the variations on the ratio of mannose to galactose in the seeds of Phaseolus angularis, 17 local strains (Yangyang, Pyeongchang, Ganghwa, Pocheon, Geumsan, Seocheon, Jincheon, Danyang, Tongyeong, Sancheong, Gumneung, Wolseong, Wando, Gokseong, Okgu, Jangsu, Bukjeju), which are located from
, were selected according to their latitudes and geographical distances. The seeds of these strains were collected and their contents of mannose and galactose were analyzed. Mannose contents in the seeds were variable, ranging from 17.071 mg/g at its highest (Jangsu) and 6.488 mg/g at its lowest (Geumsan). The contents of galactose also showed remarkable differences, ranging from 9.477 mg/g (Wolseong) to 19.877 mg/g (Jangsu). The local strains were classified into 3 variation types - coastal type I (Wando, Okgu, Bukjeju), the inland type (Jangsu, Weolseong, Danyang, Geumneung, Pyeongchang, Sancheong) and coastal type II (Ganghwa, Seocheon, Tongyeong, Jincheon), as well as 4 strange strains (Gokseong, Yangyang, Pocheon, Geumsan) according to the geographical climatic type and the ratio of mannose to galactose, which indicate the hardness of seeds in Leguminosae and ranged from 0.64 to 1.22. The variation types are very significant genecologically as evidence for microevolution related to natural and artificial selection in cultivated plants.