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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 22, Issue 12 - Dec 2012
Volume 22, Issue 11 - Nov 2012
Volume 22, Issue 10 - Oct 2012
Volume 22, Issue 9 - Sep 2012
Volume 22, Issue 8 - Aug 2012
Volume 22, Issue 7 - Jul 2012
Volume 22, Issue 6 - Jun 2012
Volume 22, Issue 5 - May 2012
Volume 22, Issue 4 - Apr 2012
Volume 22, Issue 3 - Mar 2012
Volume 22, Issue 2 - Feb 2012
Volume 22, Issue 1 - Jan 2012
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Improved Production Efficiencies of Various Adeno-Associated Virus (AAV) Serotypes and a Novel Universal AAV Titration Method
Cho, Young-Hwa ; Choi, Ye-Jin ; Yun, Jung-Hee ; Kim, Nam-Hee ; Choi, Mi-Ra ; Choi, Young-Kook ; Kim, Kyung-Hee ; Lee, Young-Ill ; Lee, Beom-Jun ; Park, Kee-Rang ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 703~712
DOI : 10.5352/JLS.2012.22.6.703
Adeno-associated virus (AAV) has been considered to be a very safe and efficient gene delivery system. However, the major obstacles to therapeutic usage of AAV have been to achieve highly efficient and reproducible production processes, and also to develop a reliable quantifying method of various serotypes with a simple protocol. We compared the efficiency of the conventional production protocol of AAV2 and adenovirus (Ad) co-infection to that of a new method containing AAV2 infection followed by pHelper transfection. We tested HEK293 and 293T, and further examined the time-dependent changes of AAV2 production. The new method of AAV2 and pHelper DNA gave about ten times higher production efficiency than that of the conventional protocol. The highest production efficiency in 293T was achieved as
virus genomes (v.g.)/cell by the new method of 10 MOI of AAV2 infection and 5 days post-infection. This protocol of the highest efficiency was then applied to produce various AAV serotypes and showed the efficiencies higher than
v.g./cell. Next, we designed the universal PCR primers of highly conserved regions for various AAV serotypes to develop a simple and reliable titration method. The universal primers could amplify all the tested AAV serotypes with similar sensitivities by ten molecular copies. Therefore, this pair of universal primers can be further utilized to detect AAV contaminants in therapeutic adenoviral vectors.
Protein Expression in Pig Species Longissimus dorsi Muscles among Different Breeds and Growth Stages
Kim, Byung-Uk ; Kim, Sam-Woong ; Hong, Yeon-Hee ; Jeong, Mi-Ae ; Ryu, Yeon-Sun ; Park, Hwa-Chun ; Jung, Jong-Hyun ; Kwon, Young-Min ; Choi, In-Soon ; Lee, Sang-Suk ; Kim, Chul-Wook ; Cho, Kwang-Keun ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 713~722
DOI : 10.5352/JLS.2012.22.6.713
When proteins extracted from longissimus dorsi muscles of Landrace and Berkshire at the finishing stage were compared by 2-DE, the Landrace demonstrated a quantitative increase in proteins related to slow skeletal muscle function, such as serum albumin precursor, troponin T (slow skeletal muscle; sTnT) and myoglobin. In contrast, the Berkshire exhibited comparatively elevated enzymes involved in metabolic pathways, fast skeletal muscle function, and energy production, such as heat shock 27-kDa protein (HSP27)-1, TnT (fast skeletal muscle; fTnT), muscle creatine kinase, phosphoglucomutase 1 (PGM1), triosephosphate isomerase (Tpi1) and adenylate kinase isoenzyme 1 (AK1). When compared to growing Berkshire, finishing Berkshire showed increased levels of aldehyde dehydrogenase 1 family, member L1 (ALDHL1), and muscle creatine kinase. In contrast, the growing Berkshire muscle had elevated levels of HSP27-1, sTnT, fTnT, serum albumin precursor, PGM1, AK1, and Tpi 1 as compared to the finishing Berkshire. The Landrace longissimus dorsi muscle may be composed of slower skeletal muscle, whereas Berkshire is composed of a faster skeletal muscle. The uniquely elevated quantities of proteins involved in skeletal muscle function, energy metabolism, and cytoskeleton function in the growing Berkshire indicate that these factors support growth and maintenance during the growing stage when compared with the finishing Berkshire.
Filter Plate Micro Trap as a Device for in situ Cultivation for Environmental Microorganisms
Jung, Da-Woon ; Ahn, Tae-Seok ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 723~729
DOI : 10.5352/JLS.2012.22.6.723
Filter plate microbial trap (FPMT) was invented as an in situ cultivation device for the isolation of bacteria from natural environments. FPMT consists of a medium and membrane filters (0.45
pore size) and microorganisms and compounds can be moved freely moved into the medium. This device was applied to two soil samples of Greenland. The microbial diversity of both soil samples by FPMT was higher than that by the conventional Petri dish-based method. Moreover, novel bacterial species were isolated by FPMT. The new FPMT is effective for in situ cultivation of natural samples and could be applicable to the isolation of uncultivable microorganism.
Study on Cosmeceutical Activities and Anti-inflammatory Activities of Magnolia biondii Extracts
Kim, Young-Hun ; Sung, Ji-Yeon ; Seo, Kyo-Seong ; Shin, Jae-Cheon ; Kim, Byung-So ; Yeum, Jeong-Hyun ; Lee, Jin-Tae ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 730~735
DOI : 10.5352/JLS.2012.22.6.730
Existing pharmaceutical studies show that Magnolia biondii is effective in treating rhinitis and in reducing cholesterol, given its endogenous, volatile ingredients. The study herein seeks to assess the cosmeceutical activities and anti-inflammatory activities of Magnolia biondii extracts for possible application as cosmetic ingredients. The cosmeceutical and anti-inflammatory activities were investigated using hydroxyl radical scavenging, superoxide dismutase (SOD)-like activity, xanthine oxidase (XO) inhibition, cell viability, nitric oxide (NO) inhibition, and inducible nitric oxide synthase (iNOS) expression by Western blotting. Magnolia biondii extracts were identified to have antioxidant activities in hydroxyl free radical scavenging, SOD-like activity, and XO inhibition. In testing the anti-inflammatory activities of the extracts, NO production was inhibited in a dose-dependent manner. Additionally, in a dose-dependent manner, the Magnolia biondii extracts were able to suppress iNOS expression in LPS-stimulated RAW 264.7 macrophage cells. From these results, Magnolia biondii showed adequate potential for application in cosmetic production and related industries as well as a functional material.
A Study on Food Habits of the Otter, Lutra lutra, and Effects of Construction of the Busan New Port on its Prey
Choi, Jun-Woo ; Yoon, Myung-Hee ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 736~743
DOI : 10.5352/JLS.2012.22.6.736
The aim of the study is to investigate the food habits of the Eurasian otter, Lutra lutra, and to examine any effects of the construction of the Busan New Port (BNP) on the prey. The frequencies and bulk estimate ratios of the biological debris, taken from spraints collected seasonally during the period from 2005 to 2011 at 16 areas, were analyzed. As the results, it was demonstrated that otters prey mainly on fish but occasionally on crustaceans and gastropods, etc., and the Mugiliformes was the most preferred fish. Although fish debris was observed throughout the season, it was suggested that the amount of fish eaten by the otter seemed to be associated with the amount of fish inhabiting the study area, judging from the striking similarities in the pattern of seasonal variations in each order of the fish between the frequency from the spraints and the fish catch. On the other hand, the frequencies of most of the fish, crustaceans, and gastropods from the spraints decreased from 2010, indicating the decrease of the amount of the prey by the construction of BNP and the strong possibility of the decrease in the number of otters in the near future. This is supported by the other studies, such as one showing a sudden decrease in the number of spraints since 2010, recent decrease in the fish catch, and the increase of marine pollution at this study area.
The Effect of Moderate Aerobic Exercise on Adiponectin, Retinol Binding Protein-4, and Vascular Inflammation Factors in Obese Children
Lee, Sung-Soo ; So, Yong-Seok ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 744~750
DOI : 10.5352/JLS.2012.22.6.744
The aim of the present study is to investigate the effects of 12 weeks-moderate aerobic exercise training on body composition, adiponectin, retinol-binding protein-4 (RBP-4), and vascular inflammation factors in obese children. The subjects were classified into two groups, one of which was an exercise group (n=15) practicing moderate aerobic exercise training for 12 weeks, and the other group, the control group (n=13), was not in that program. The exercise group participants performed on the treadmill running at heart rate reserve (HRR) 50% of exercise intensity for 300 kcal of the consumed time. The results of all the studies and inspections are as follows: Weight, body mass index, body fat, and systolic and diastolic blood pressure were significantly lower, while
max was higher in the exercise group than in the control group (p<0.05, respectively). Fasting glucose, insulin, and HOMA-IR levels in the exercise group were significantly decreased (p<0.05), whereas adiponectin levels in the exercise group were significantly increased after 12 weeks' exercise training (p<0.05). In addition, our results showed that RBP4, C-reactive protein (CRP), and interleukin-6 (IL-6) concentrations in the exercise group were significantly lower than those of the control group (p<0.05). This investigation has shown that a 12 weeks-regular aerobic exercise program offers useful effects such as an amelioration of inflammation and body indices in obese children.
α-Glucosidase, Tyrosinase, and Elastase Inhibitory Effects of Enzymatic Extracts from Ecklonia cava and its Alcohol Metabolizing Activity
Kim, Hye-Youn ; Cho, Eun-Kyung ; Kang, Su-Hee ; Bae, Jeong-Mi ; Choi, Young-Ju ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 751~759
DOI : 10.5352/JLS.2012.22.6.751
Microbulbifer sp. was used to acquire the degrading products from Ecklonia cava (DPEC) and the products were investigated to determine the physiological activities. Firstly, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity and superoxide dismutase (SOD) assay were about 84.1% and 89.6% at 2.5 mg/ml, respectively. In addition, nitrite scavenging ability was shown to be 56.3% at 0.5 mg/ml on pH 1.2.
-Glucosidase inhibitory activity was increased in a dose-dependent manner and was about 58.7% at 2.5 mg/ml. To determine the influence of DPEC on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. Facilitating rates of ADH and ALDH activities by DPEC were 123.3% and 215.2% at 2.5 mg/ml, respectively. For analyses of anti-wrinkling and whitening effects, its elastase and tyrosinase inhibitory activities were measured and were about 73.1% and 42.2% at 2.5 mg/ml, respectively. These results indicated that DPEC has valuable biological attributes owing to its antioxidant, nitrite scavenging, and alcohol metabolizing activities and
-glucosidase, elastase, and tyrosinase inhibitory activities.
Induction of Apoptosis and Antitumor Activity by Stichoposide D through the Generation of Ceramide in Human Leukemia Cells
Park, Eun-Seon ; Yun, Seung-Hoon ; Shin, Sung-Won ; Kwak, Jong-Young ; Park, Joo-In ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 760~771
DOI : 10.5352/JLS.2012.22.6.760
Marine triterpene glycosides are physiologically active natural compounds isolated from sea cucumbers(holothurians). It was demonstrated that they have a wide range of biological activities, including antifungal, cytotoxic, and antitumor effects. A previous study showed that stichoposide C (STC) isolated from Thelenota anax induces apoptosis through generation of ceramide by activation of acid sphingomyelinase (SMase) and neutral SMase in human leukemia cells. In this study, we investigated whether STD, a structural analog of STC, can induce apoptosis and examined the molecular mechanisms for its activity. It was found that STC and STD induce apoptosis in a dose- and time-dependent manner and lead to the activation of caspase-8, mitochondrial damage, activation of caspase-9, and activation of caspase-3 in K562 and HL-60 cells. STC activates acid SMase and neutral SMase, which results in the generation of ceramide. Specific inhibition of acid SMase or neutral SMase partially blocked STC-induced apoptosis, but not STD-induced apoptosis. In contrast, STD generates ceramide through the activation of ceramide synthase. Specific inhibition of ceramide synthase partially blocked STD-induced apoptosis, but not STC-induced apoptosis. Moreover, STC and STD markedly reduced tumor growth of HL-60 xenograft tumors and increased ceramide generation in vivo. These results indicate that STC and STD can induce apoptosis and have antitumor activity through the different molecular mechanisms, because they have a different sugar residue attached to aglycones. Thus, these results suggest that their actions are affected by a sugar residue attached to aglycones and they can be used as anticancer agents in the treatment of leukemia.
Effects of Black Garlic Supplementation and Exercise on TBARS, HSP 70 and COX-2 Expression after High-intensity Exercise
Baek, Yeong-Ho ; Lee, Sang-Ho ; Han, Min-Ho ; Choi, Yung-Hyun ; Kim, Seung-Hyun ; Kwak, Yi-Sub ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 772~777
DOI : 10.5352/JLS.2012.22.6.772
The purpose of this study was to investigate the effects of black garlic supplementation and exercise on TBARS, HSP 70, and COX-2 expression after high-intensity exercise in rats. Twenty-four male Sprague-Dawley rats (4 weeks) were used in this study. Experimental groups were a control group (A, n=6), black garlic supplementation group (B, n=6), exercise group (C, n=6), and black garlic supplementation with exercise group (D, n=6). High-intensity exercise was performed by treadmill running (every 10 min speed increased gradually; 15~24 m/min,
grade) and 2.86 g/kg black garlic for 4 weeks. The findings of this study were as follows: TBARS levels were significantly lower in the D group compared to the other group and B and C groups were significantly lower in TBARS levels than in the A group. HSP 70 expression was lower in the B group compared to the other groups. COX-2 expression has showed a similar expression in all groups. Therefore, it is considered that black garlic intake and exercise may reduce oxidative stress.
The Reverse Effect of Salicylic Acid on Cd-induced Growth, Chlorophyll, and Rubisco/Rubisco Activase in Tobacco
Wang, Yu Shan ; Roh, Kwang-Soo ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 778~787
DOI : 10.5352/JLS.2012.22.6.778
The influence of salicylic acid (SA) on growth, chlorophyll, and rubisco/rubisco activase and effect of denaturator on rubisco/rubisco activase activity were studied in tobacco plants grown in vitro with cadmium (Cd) treatment. In order to find out the optimum concentration of SA, tobacco plants treated with
mM of SA were grown in MS medium for 9 weeks, respectively. The most pronounced effect on in vitro growth was found at
mM SA. Among the control (not treated with Cd and SA), SA, Cd, and Cd + SA, the growth and content of chlorophyll were in the sequence of Cd < Cd + SA < control < SA, and significantly higher at SA compared with others. Similar results were also observed in the content and activity of rubisco and rubisco activase. These data suggest that inhibitory effect by Cd was reversed by SA. These results also indicate that SA has a positive effect on Cd. The effect of denaturants on rubisco activity showed in the sequence of Cd < Cd + SA < control < SA. Rubisco activity was promoted by L-cysteine and
-mercaptoethanol, not by urea, thiourea, and guanidium-HCl. These data suggest that urea, thiourea, and guanidium-HCl are able to act as denaturator, and L-cysteine and
-mercaptoethanol are not. None of the five denaturants affected the activity of rubisco activase.
Effects of Natural Compounds from Various Plant Eradicate the Persister Cell of Edwardsiella tarda Treated with Antibiotics of Florfenicol and Amoxicillin
Kim, Na-Kyoung ; Kweon, Dae-Hyuk ; Kim, Sung-Koo ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 788~793
DOI : 10.5352/JLS.2012.22.6.788
High concentration of antibiotics has been used to treat the outbreak of edwardsiellosis caused by Edwardsiella tarda in aquaculture. However, not all of the bacteria have been killed with high concentrations of antibiotics treatment by the formation of persister cells with a dormant state. The main objective of this study was to kill persister cell using antibiotics with the addition of natural plant compounds. Antibiotics used in this study consist of 100 mg/ml florfenicol and 100 mg/ml amoxicillin. Ten natural plant compounds with persister cell inhibitor activity to E. coli were obtained from Protein Engineering and Systems Biology Lab. of Sungkyunkwan University. The persister cell inhibition activities of those natural plant compounds were evaluated in test tube. Concentrations of the antibiotics were in the ranges of 25~200
. The persister cell formation was observed after 16 hours of culture. Persister cells were killed by antibiotics with natural plant compounds. Among ten natural plant compounds, Gynostemma pentaphyllum, Mallotus japonicus, and Orixa japonica showed persister cell formation inhibition activities. The optimal concentrations of G. pentaphyllum, M. japonicus, and O. japonica for the inhibitor of persister cell formation were 100
, and 200
, respectively. In vivo study was carried out to evaluate the effect of the antibiotics with natural plant compounds using aquacultural fish, olive flounder, as test animals. G. pentaphyllum, M. japonicus, and O. japonica of 30
, and 10
with antibiotics reduced cumulative mortalities, showing the effectiveness of persister cell inhibition.
Seroprevalence of Paratuberculosis in Pure-bred Breeding Cattle in Korea
Kim, Ha-Young ; Byun, Jae-Won ; Jeon, Albert Byung-Yun ; Park, Bum-Soo ; Jung, Ji-A ; Park, Mi-Hak ; Lim, Yeon-Su ; Jung, Byeong-Yeal ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 794~798
DOI : 10.5352/JLS.2012.22.6.794
Paratuberculosis (Johne's disease), a chronic wasting disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a major cause of economic loss in the cattle industry. In Korea, national monitoring of breeding stock for MAP has been implemented. In this study, we report the results of serological testing to determine the prevalence of MAP in breeding stock of Korean native and dairy cattle during 2008 and 2009. A total of 3,927 serum samples were submitted (3,692 Korean native cattle and 235 dairy cattle) to Animal Disease Diagnostic Division, Animal, Plant and Fisheries Quarantine and Inspection Agency. The samples were classified into four different age groups for MAP; group 1 (
year, n = 1,509), group 2 (>2 years to
years, n = 486), group 3 (>3 years to
years, n = 441), and group 4 (>4 years, n = 1,491). Overall seroprevalence of MAP in this study was 0.5% (21/3,927), which was much lower than that of conventional cattle (1.2-16.4%) in Korea. Also, the seroprevalence was determined by age groups: three of group 1 (0.2%), two of group 2 (0.4%), three of group 3 (0.7%), and 13 of group 4 (0.9%) were seropositive for MAP, respectively. Although seropositive samples were found in all age groups, the seroprevalence tended to increase with age. Our study showed that the seroprevalence of MAP in pure-bred breeding dairy cattle (0%) was lower than that in pure-bred breeding Korean native cattle (0.6%).
Growth Inhibition of Mushroom Pathogen by Bacillus sp. HJ 57
Seo, Kwon-Il ; Gal, Sang-Won ; Yee, Sung-Tae ; Park, Kyung-Wuk ; Lee, Sang-Won ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 799~806
DOI : 10.5352/JLS.2012.22.6.799
Approximately 80 species of bacteria were isolated from the fermented mushroom first and the HJ-57 antibacterial micro-organism was selected to the final isolation bacteria. It has a high degree of CMCase, amylase, and protease activity as well as high antibacterial activity against mushroom pathogenic bacteria without affecting the growth and development of Flammulina velutipes and Lentinus edodes mushrooms. The finally selected HJ-57 antibacterial micro-organism was identified as Bacillus sp. HJ-57. The initial pH for culture was pH7 and its optimum culture temperature was
. The antibacterial material produced by Bacillus sp. HJ-57 showed a little antibacterial activity even in the 12 hr of culture, but showed the highest antibacterial activity in the 36~48 hr of culture. The HJ-57 antibacterial micro-organism also showed a high antibacterial activity against mushroom pathogenic bacteria and molds in the corn cob contained culture medium is used in Flammulina velutipes cultivators.
Study on the Antioxidative and Physiological Activities of Saururus chinensis Extract
Kang, Chang-Soo ; Lee, Min-Joo ; Park, Cheol-Bem ; Bang, In-Seok ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 807~814
DOI : 10.5352/JLS.2012.22.6.807
Saururus chinensis has long been widely used in oriental folk medicines to treat diseases. In the current study, organic solvent fractions obtained from the main methanolic extract of S chinensis were evaluated for their antioxidative and related physiological activities. The antioxidant activity of the fractions was measured using DPPH free radical scavenging activity, increased in a dose-dependent manner, and the
of the ethyl acetate fractions exhibited a value of 12.84
higher than 27.22
compared to the BHT. Also, the cell viability of S. chinensis on
-induced HDF cell death (
) showed the highest cell viability of 89.39% in 50
of ethyl acetate fraction and 67.98% of visible cell survival rate in n-butanolic fraction. Meanwhile, all fractions of the S. chinensis extract led to a slight down regulation of the mRNA expression of fibulin-5, which is related to skin elasticity, and the ethyl acetate fraction having high antioxidant activity showed a markedly inhibitory effect on chick embryonic angiogenesis using the CAM assay. These results suggest that the ethyl acetate fraction of S. chinensis extract could be a good material in therapeutic application for antioxidant and related anti-angiogenesis activities.
Induction of Apoptosis and Inhibition of NO Production by Piceatannol in Human Lung Cancer A549 Cells
Choi, Yung-Hyun ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 815~822
DOI : 10.5352/JLS.2012.22.6.815
Piceatannol (trans-3,4,3',5'-tetrahydroxystilbene), a natural stilbene, is an analogue of resveratrol. Although recent experimental data have revealed the health benefit potency of piceatannol, the molecular mechanisms underlying the anti-cancer activity have not yet been studied in detail. In the present study, the further possible mechanisms by which piceatannol exerts its pro-apoptotic action in cultured human lung cancer A549 cells were investigated. Exposure of A549 cells to piceatannol resulted in growth inhibition and induction of apoptosis. Apoptosis induction of A549 cells by piceatannol showed correlation with proteolytic activation of caspase-3, -8, and -9, and concomitant degradation of activated caspase-3 target proteins such as poly (ADP-ribose) polymerase, phospholipase C-
-catenin, and Inhibitor caspase-activated DNase. The increase in apoptosis by piceatannol treatment was also associated with an increase of pro-apoptotic Bax expression and decrease of anti-apoptotic Bcl-2 and Bcl-xL expression, and caused down-regulation of the inhibitor of apoptosis protein family members and up-regulation of Fas and Fas legend. In addition, piceatannol treatment markedly inhibited the expression of mRNA and proteins of inducible nitric oxide (NO) synthase, and the levels of NO production were progressively down-regulated by piceatannol treatment in a dose-dependent fashion. The results indicate that piceatannol may have therapeutic potential against human gastric cancer cells.
Effect of Tryptic Soy Broth (TSB) and Luria-Bertani (LB) Medium on Production of Subtilisin CP-1 from Bacillus sp. CP-1 and Characterization of Subtilisin CP-1
Park, Chang-Su ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 823~827
DOI : 10.5352/JLS.2012.22.6.823
A bacterial strain producing a fibrinolytic enzyme, subtilisin CP-1, was isolated from Doen-Jang, a Korean traditional fermentation food. Based on the analysis of gene sequence of 16S rRNA and biochemical analysis, the strain was identified as Bacillus sp. and named as Bacillus sp. CP-1. To investigate the effect of the medium on the production of fibrinolytic enzyme from Bacillus sp. CP-1, two commercial bacterial culture media, tryptic soy broth (TSB) and Luria-Bertani (LB), were applied to the cultivation of Bacillus sp. CP-1. The strain secreted only one proteolytic enzyme (subtilisin CP-1) in the culture broth. The molecular weight of subtilisin CP-1 was estimated to be 28 kDa. Subtilisin CP-1 was optimally active at pH 9.0 and
, and exhibited high specificity for Meo-Suc-Arg-Pro-Tyr-pNA (S-2586), a synthetic chromogenic substrate for chymotrypsin. The first eight amino acid residues of the N-terminal sequence of the enzyme are AQSVPYGI; this sequence is identical to that of subtilisin NAT and E.
Effect of Flammulina velutipes Extracts Cultivated with Oriental Herbal Plants on the Activation of Immune Cells
Kim, Jong-Jin ; Lee, Sang-Won ; Park, Kyung-Wuk ; Seo, Kwon-Il ; Yee, Sung-Tae ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 828~836
DOI : 10.5352/JLS.2012.22.6.828
The objective of the current study was to determine the effects of the extracts isolated from the fruit body of Flammulina velutipes cultivated with oriental herbal plants on mouse splenocytes, B cells, and macrophages in vitro. The ethanol extracts B (EEB) directly induced the proliferation of spleen cells in a dose-dependent manner and increased IL-6, TNF-
, and IFN-
synthesis. The EEB also increased the proliferation of B cells in a dose-dependent manner. The production of immunoglobulin M, G1, G2a, G2b, and IgG3 in the presence of the EEB increased progressively in the culture supernatant. When the EEB were used in macrophage cell line (RAW264.7) stimulation, there was a marked induction of NO synthesis in a dose-dependent manner and an increased IL-6, TNF-
, and GM-CSF synthesis. Intraperitoneal injection with EBB showed life prolongation effect of 16.1% in mice previously inoculated with sarcoma-180, respectively. These results suggest that the capacity of the EEB isolated from the fruit body of Flammulina velutipes cultivated with oriental herbal plants seems to act as a potent immunomodulator causing augmentation of immune cell activity, and with the absence of notable side-effects, Flammulina velutipes EEB could be used as a biological response modifier having possible therapeutic effects against immunological disorders. This study also showed that functional components of Flammulina velutipes were possibly improved by incorporating oriental herbal plants in a growth medium.
Comparison of Endo-, Exo-Cellular Enzyme Activity for New Strains of Hypsizygus marmoreus
Lee, Chang-Yun ; Song, Ho-Sung ; Ro, Hyeon-Su ; Woo, Ju-Ri ; You, Young-Hyun ; Kim, Jong-Guk ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 837~843
DOI : 10.5352/JLS.2012.22.6.837
This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the
cultivation experiment, and selected six strains from one of 58 strains through the
cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of
-amylase at the highest as 73.9~102.2 unit/mg protein, and chitinase is lower than
-amylase at 8.1~13.1 unit/mg protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of
-amylase is the highest at 5,292~1,184 unit/mg protein, and CMCase and xylanase were 1,140~245 unit/mg protein, 94~575 unit/mg protein, compared to each other. However, the enzyme activity of
-glucosidase and chitinase is low.
Antioxidant and Cancer Cell Growth Inhibition Activity of Five Different Varieties of Artemisia Cultivars in Korea
Kim, Ra-Jeong ; Kang, Min-Jung ; Hwang, Cho-Rong ; Jung, Woo-Jae ; Shin, Jung-Hye ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 844~851
DOI : 10.5352/JLS.2012.22.6.844
Antioxidant and cancer cell growth inhibition activity of hot water extract from five different varieties of Artemisia (A. Argyi H., A. iwayomogi Kitamura, A. Princeps Var Orien talis HARA, A. princeps Pampanini and A. annua L.) in Korea was studied. We determined the phenol and flavonoid contents and examined antioxidant assay, such as DPPH, NO radical scavenging, activity ferric-reducing antioxidant power (FRAP), and bleaching inhibition activity in the
-carotene linolic acid system. Also, we performed HeLa and MCF-7 cancer cell growth inhibition assay of Artemisia extracts. Total phenol and flavonoid contents were the highest in A. iwayomogi Kitamura followed by A. Argyi H. DPPH radical scavenging activity was the highest in A. Argyi H. at 50
concentration, NO radical scavenging activity was more than 50% in A. Princeps Var Orien talis HARA, A. princeps Pampanini, and A. annua L. at 200
concentration. FRAP was higher in A. Argyi H. and A. iwayomogi Kitamura. Antioxidant activity in the
-carotene linolinolic system was also higher in A. Argyi H. and A. iwayomogi Kitamura by 60.50% and 56.90% at 100
concentration, respectively. In cancer cell growth inhibition activities at 400
concentration, A. iwayomogi Kitamura showed greater than 80% on HeLa cell. A. princeps Pampanini and A. Argyi H. extract had growth inhibition activities greater than 80% on MCF cell. The results of this study suggest that the antioxidant and anticancer activities in various Artemisia are a promising source of functional food ingredients.
In vivo Anti-inflammatory, Antipyretic, and Analgesic Activities of the Aquaculturable Green Seaweed Codium fragile Extracts in Mice
Kang, Ji-Young ; Luyen, Quoc-Hai ; Khan, Mohammed Nurul Absar ; Choi, Jae-Suk ; Choi, In-Soon ; Hong, Yong-Ki ;
Journal of Life Science, volume 22, issue 6, 2012, Pages 852~856
DOI : 10.5352/JLS.2012.22.6.852
Dichloromethane, ethanol, and boiling water extracts of the green seaweed Codium fragile, used as an herbal medicine and known as an invasive species over the world, were examined for anti-inflammatory, antipyretic, and analgesic activities in mice. The dichloromethane and ethanol extracts inhibited inflammatory symptoms of mouse ear edema and erythema by 74% or higher. The extracts also demonstrated inhibition of pyrexia, similar to that of acetyl salicylic acid. Eicosapentaenoic acid was isolated from the seaweed as the main active anti-inflammatory compound. These findings are consistent with various claims that the seaweed can be used as remedies for inflammation-related symptoms.