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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 23, Issue 12 - Dec 2013
Volume 23, Issue 11 - Nov 2013
Volume 23, Issue 10 - Oct 2013
Volume 23, Issue 9 - Sep 2013
Volume 23, Issue 8 - Aug 2013
Volume 23, Issue 7 - Jul 2013
Volume 23, Issue 6 - Jun 2013
Volume 23, Issue 5 - May 2013
Volume 23, Issue 4 - Apr 2013
Volume 23, Issue 3 - Mar 2013
Volume 23, Issue 2 - Feb 2013
Volume 23, Issue 1 - Jan 2013
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Genetic Diversity and Phenetic Relationship of Dill (Anethum graveolens L.) by rps16-trnK DNA Sequences
Sung, Jung-Sook ; Chung, Jong-Wook ; Lee, Gi-An ; Kang, Man-Jung ; Lee, Sok-Young ; Huh, Man-Kyu ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1305~1310
DOI : 10.5352/JLS.2013.23.11.1305
Dill (Anethum graveolens L.) is an annual herb with a long history and it is mainly used as a spice and as a medicine that is effective as a digestive aid, a sedative, and a narcotic, and that helps remove bad breath. Dill grows wild in the districts along the shores of the Mediterranean Sea, West Asia, China, and Korea. An estimate of the phylogenetic relationships within dill accessions in 20 countries was inferred using data from the rps16-trnK3-intergenic spacer. The aligned data sets for dill ranged from 747 to 779 nucleotides (bp) as a result of the differences in the insert/delete nucleotides. The sequence variation within the dill accessions was mostly due to nucleotide substitutions, although several small insertions and deletions can be found. Among 100 accessions from 20 countries, the Eastern Asia accessions were more closely related to the North American accessions than to the Central Asia and European accessions. Although some accessions were not congruent completely with geographical locations, the dill accessions with rps16-trnK analysis resulted in plants with better-resolved clades.
Identification of the Interaction between Insulin-like Growth Factor Binding Protein-4 (IGFBP-4) and Heterogeneous Nuclear Ribonucleoprotein L (hnRNP L)
Choi, Mieyoung ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1311~1316
DOI : 10.5352/JLS.2013.23.11.1311
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a major pre-mRNA binding protein and it is an abundant nuclear protein that shuttles between the nucleus and the cytoplasm. hnRNP L is known to be related to many cellular processes, including chromatin modification, pre-mRNA splicing, mRNA export of intronless genes, internal ribosomal entry site (IRES)-mediated translation, mRNA stability, and spermatogenesis. In order to identify the cellular proteins interacting with hnRNP L, this study performed a yeast two-hybrid screening, using a human liver cDNA library. The study identified insulin-like growth factor binding protein-4 (IGFBP-4) as a novel interaction partner of hnRNP L in the human liver. It then discovered, for the first time, that hnRNP L interacts specifically with IGFBP-4 in a yeast two-hybrid system. The authenticity of this two-hybrid interaction of hnRNP L and IGFBP-4 was confirmed by an in vitro pull-down assay.
Development of Marker-free Transgenic Rice for Increasing Bread-making Quality using Wheat High Molecular Weight Glutenin Subunits (HMW-GS) Gene
Park, Soo-Kwon ; Shin, DongJin ; Hwang, Woon-Ha ; Oh, Se-Yun ; Cho, Jun-Hyun ; Han, Sang-Ik ; Nam, Min-Hee ; Park, Dong-Soo ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1317~1324
DOI : 10.5352/JLS.2013.23.11.1317
High-molecular weight glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the wheat grain. We have produced marker-free transgenic rice plants containing a wheat Glu-1Bx7 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using the Agrobacterium-mediated co-transformation method. The Glu-1Bx7-own promoter was inserted into a binary vector for seed-specific expression of the Glu-1Bx7 gene. Two expression cassettes comprised of separate DNA fragments containing only Glu-1Bx7 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Glu-1Bx7 or HPTII was infected to rice calli at a 3:1 ratio of Glu-1Bx7 and HPTII, respectively. Then, among 216 hygromycin-resistant
plants, we obtained 24 transgenic lines with both Glu-1Bx7 and HPTII genes inserted into the rice genome. We reconfirmed integration of the Glu-1Bx7 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat Glu-1Bx7 were stably expressed in the rice
seeds. Finally, the marker-free plants harboring only the Glu-1Bx7 gene were successfully screened at the
Bioinformatic Analysis of the Canine Genes Related to Phenotypes for the Working Dogs
Kwon, Yun-Jeong ; Eo, Jungwoo ; Choi, Bong-Hwan ; Choi, Yuri ; Gim, Jeong-An ; Kim, Dahee ; Kim, Tae-Hun ; Seong, Hwan-Hoo ; Kim, Heui-Soo ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1325~1335
DOI : 10.5352/JLS.2013.23.11.1325
Working dogs, such as rescue dogs, military watch dogs, guide dogs, and search dogs, are selected by in-training examination of desired traits, including concentration, possessiveness, and boldness. In recent years, genetic information has been considered to be an important factor for the outstanding abilities of working dogs. To characterize the molecular features of the canine genes related to phenotypes for working dogs, we investigated the 24 previously reported genes (AR, BDNF, DAT, DBH, DGCR2, DRD4, MAOA, MAOB, SLC6A4, TH, TPH2, IFT88, KCNA3, TBR2, TRKB, ACE, GNB1, MSTN, PLCL1, SLC25A22, WFIKKN2, APOE, GRIN2B, and PIK3CG) that were categorized to personality, olfactory sense, and athletic/learning ability. We analyzed the chromosomal location, gene-gene interactions, Gene Ontology, and expression patterns of these genes using bioinformatic tools. In addition, variable numbers of tandem repeat (VNTR) or microsatellite (MS) polymorphism in the AR, MAOA, MAOB, TH, DAT, DBH, and DRD4 genes were reviewed. Taken together, we suggest that the genetic background of the canine genes associated with various working dog behaviors and skill performance attributes could be used for proper selection of superior working dogs.
Inhibitory Efficacy of Angelica gigas Nakai on Microphthalmia-associated Transcription Factor (MITF), Tyrosinase Related Protein-1 (TRP-1), Tyrosinase Related Protein-2 (TRP-2), and Tyrosinase mRNA Expression in Melanoma Cells (B16F10)
Lee, Soo-Yeon ; Lee, Jin-Young ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1336~1341
DOI : 10.5352/JLS.2013.23.11.1336
The purpose of this study was to research the whitening effects of the extract from Angelica gigas Nakai, which is one of the most widely used herbal medicines in Asia. For whitening effects, the tyrosinase inhibition effect of the A. gigas Nakai extract was shown to be greater than 70% at 1,000
concentration. The result of measuring the cell toxicity effect of the extract from A. gigas Nakai on melanoma cells showed 99% toxicity at 500
concentration. The microphthalmia-associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), and tyrosinase mRNA expression inhibitory effect by reverse transcription-PCR of the extract from A. gigas Nakai were decreased by 85.7%, 123.9%, 68.8%, and 208%, respectively, at 50
concentration. All these findings could verify that extract from A. gigas Nakai could have an effect on whitening. Moreover, extract from A. gigas Nakai has great potential as a cosmetic ingredient.
Protection of Primary Cultured Mouse Hepatocytes from Chemical Hypoxia-induced Injury by Hydrogen Sulfide
Lee, Min Young ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1342~1350
DOI : 10.5352/JLS.2013.23.11.1342
We examined the effect of hydrogen sulfide (
) in chemical hypoxia-induced injury in mouse hepatocytes. Cell viability was significantly decreased by cobalt chloride (
), a well-known hypoxia mimetic agent in a time- and dose- dependent manner. Sodium hydrosulfide (NaHS, a donor of
) pretreatment before exposure to
significantly attenuated the
-induced decrease of cell viability.
treatment resulted in an increase of intracellular ROS generation, which is inhibited by NaHS or N-acetyl-cysteine (NAC, a ROS scavenger), and p38 MAPK phosphorylation, which is also blocked by NaHS or NAC. The
-induced increase of the Bax/Bcl-2 ratio was attenuated by NaHS, NAC, and SB 203580 (p38 MAPK inhibitor). The
-induced decrease of cell viability was also attenuated by NaHS, NAC, and SB 203580 pretreatment. Additionally, NaHS inhibited the
-induced COX-2. Similar to the effect of NaHS, NAC blocked
-induced COX-2 expression. Furthermore, NS-398 (a selective COX-2 inhibitor) attenuated not only the
-induced increase of the Bax/Bcl-2 ratio, it also decreased cell viability. Taken together,
protects primary cultured mouse hepatocytes against
-induced cell injury through inhibition of the ROS-activated p38 MAPK cascade and the COX-2 pathway.
Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells
Lee, Sun Min ; Kim, Young Sook ; Jang, Wook Jin ; Rakib, Abdur Md. ; Oh, Tae Woo ; Kim, Boh Hyun ; Kim, So Young ; Kim, Jeong Ok ; Ha, Yeong Lae ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1351~1359
DOI : 10.5352/JLS.2013.23.11.1351
The anti-proliferative activity of
-ionone was investigated on human non-small lung cancer A-549 cells (designated A-549 cells). A-549 cells were treated with various concentrations of
-ionone (1, 5, 10, and 15
) for two, four, and six days. Biochemical markers related to the growth inhibition of A-549 cells by
-ionone were measured at the second day of incubation.
-Ionone inhibited the growth of A-549 cells by dose-and time-dependent manners, resulting in an
at the second day of incubation.
-Ionone induced apoptosis by a dose-dependent manner.
-Ionone increased levels of p53, p21, and Bax proteins, but suppressed expression of the Bcl-2 protein. Similarly,
-ionone enhanced cytochrome c release from the mitochondria to the cytosol, and induced activation of caspase-9 and -3. Additionally,
and COX-2 protein levels. These results suggest that the
-ionone inhibits the proliferation of A-549 cells through reciprocal regulation of Bax and Bcl-2 gene expression and suppression of
and COX-2 protein expressions.
Optimization of a Process for Extraction of Petasin from Petasites japonicus Leaves by Response Surface Methodology
Lee, Dong Wan ; Lee, Se Yeul ; Chung, Hun Sik ; Choi, Young Whan ; Im, Dong Soon ; Lee, Young Guen ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1360~1364
DOI : 10.5352/JLS.2013.23.11.1360
Petasin extracted from Petasites japonicus leaves has been well known to be effective in the treatment of allergic asthma. This study was carried out to optimize the extraction process of petasin from P. japonicus leaves by response surface methodology (RSM). The dried powder of P. japonicus leaves was extracted at ethanol concentrations ranging from 40% to 80%, extraction rpm ranging from 125 rpm to 225 rpm, and extraction time ranging from 1 to 3 hours. The effects of the extraction conditions on the dry yield and petasin content of the extracts were investigated using a second-order Box-Behnken design. The petasin content was significantly affected by ethanol concentration, extraction rpm, and extraction time, tending to increase more with increasing ethanol concentration. The optimum condition for petasin extraction from Petasites japonicus leaves was 79.92% in ethanol concentration, 178.10 rpm in extraction rpm, and 2.06 hours in extraction time, respectively.
Hydrolysis of Cellulose by Immobilized Cellulase in a Packed Bed Reactor
Kang, Byung Chul ; Lee, Jong Baek ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1365~1370
DOI : 10.5352/JLS.2013.23.11.1365
Immobilized cellulase on weak ion exchange resin showed a typical Langmuir adsorption isotherm. Immobilized cellulase had better stability with respect to pH and temperature than free cellulase. Kinetics of thermal inactivation on free and immobilized cellulase followed first order rate, and immobilized cellulase had a longer half-life than free cellulase. The initial rate method was used to characterize the kinetic parameters of free and immobilized enzyme. The Michaelis-Menten constant
was higher for the immobilized enzyme than it was for the free enzyme. The effect of the recirculation rate on cellulose degradation was studied in a recycling packed-bed reactor. In a continuous packed-bed reactor, the increasing flow rate of cellulose decreased the conversion efficiency of cellulose at different input lactose concentrations. Continuous operation for five days was conducted to investigate the stability of long term operation. The retained activity of the immobilized enzymes was 48% after seven days of operation.
Effects of Monascus-fermented Angelica gigas Nakai on the Contents of Serum Lipid and Tissue Lipid Peroxidation in Alcohol Feeding Rats
Ahn, Hee-Young ; Park, Kyu-Rim ; Kim, Yu-Ra ; Yoon, Kyoung-Hoon ; Kim, Jung-Wook ; Cho, Young-Su ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1371~1380
DOI : 10.5352/JLS.2013.23.11.1371
The effect of Monascus-fermented Angelica gigas Nakai (AFAG) on the contents of serum lipids and tissue lipid peroxidation was investigated in alcohol feeding rats (Alc group). The serum contents of total lipid and free fatty acid in the alcohol feeding rats were significantly increased, but these increases tended to decrease in the AFAG group. The content of serum triglyceride was also significantly decreased in the AFAG group compared to the other groups. The serum content of total-cholesterol was not significantly different between the normal group and the AFAG group. The content of HDL-cholesterol in serum was slightly increased in the AFAG group compared to the Alc group. The content of thiobarbituric acid reactive substances (TBARS) in the liver, heart, spleen, and testis were significantly increased in the Alc group compared to the normal group, but these increases were significantly decreased in the AFAG group. The content of liver zinc was decreased in the Alc group and it was significantly increased in the AFAG group, which suggested that the lipid peroxidation contents are inversely correlated with the liver zinc content. The hepatic glutathione concentration was significantly decreased in the Alc group, but this content was significantly increased in the AFAG group, and it showed the antioxidant ability of glutathione. These activities were also compared to the standard silymarin drug treatment. Thus, the findings of the present study indicated the significant antioxidant and antihyperlipidemic activity of Monascus-fermented Angelica gigas Nakai against ethanol-induced toxicity.
Biological Effects of the Leaves and Roots of Ligularia stenocephala
Nam, Young-Joo ; Lee, Dong-Ung ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1381~1387
DOI : 10.5352/JLS.2013.23.11.1381
The leaves and roots of Ligularia stenocephala, which are widely used as a food in Korea, were investigated for their antioxidant activities and cytotoxicity in vitro, and their hepatoprotective effect, alcohol detoxicant efficacy, and memory-enhancing property were investigated in vivo. The unique odor of the leaves was analyzed by GC-MS. Lipid peroxidation, superoxide anion formation, and DPPH radicals were inhibited remarkably by the extracts of the leaves and roots. The leaves of this edible plant significantly protected the hepatotoxicity induced by carbon tetrachloride and further diminished the blood alcohol content in mice. While the roots of this plant exhibited adequate cytotoxicity against four human tumor cell lines, especially against melanoma, the leaves revealed relatively weak activity. Both the leaves and the roots exerted an excellent ameliorating property on scopolamine-induced memory impairment in the passive avoidance task using an animal model. The hexane fraction of the leaves was analyzed by GC-MS, suggesting that a series of terpenoids may be odorous compounds in this plant.
In vivo Antitumor Activity and Acute, Subacute Toxicity of Keumsa (Phellinus linteus) Extracts
Kim, Jong-Myeung ; Park, Jun-Duck ; Park, Dong-Chan ; Kim, Byung-Oh ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1388~1396
DOI : 10.5352/JLS.2013.23.11.1388
This study used an S-180 cell-injected mouse model to evaluate the antitumor effects of the acute and subacute toxicity of Keumsa (Phellinus linteus) extract intravenously administrated in ICR mice. When administered intravenously (31.3-250 mg/kg body weight), Keumsa (Phellinus linteus) extract significantly inhibited the growth of the solid tumor cell. The antitumor activity of Keumsa (Phellinus linteus) extract increased in a dose-dependent manner. The highest dose (250 mg/kg body weight) was highly effective, reducing tumor formation by 42.7% compared with the control group. In the acute toxicity test,
of the Keumsa (Phellinus linteus) extract showed 632.84 mg/kg (♂) and 814.48 mg/kg (♀) after intravenous administration. In addition, liver and spleen weight were increased in a dose-dependent manner. In the subacute toxicity test, the mice were intravenously administered over the course of 28 days. The
of the Keumsa (Phellinus linteus) extract showed 355.41 mg/kg (♂) and 383.53 mg/kg (♀) after intravenous administration. The liver and spleen weight also increased in a dose-dependent manner. In the case of the group that received more than 125 mg/kg of intravenous administration, exercise capacity, such as jumping ability and agility, were significantly increased. These results suggest that Keumsa (Phellinus linteus) extract can be regarded as a potent enhancer of the innate immune response, and it can be considered as a new natural product with low toxicity that may be used as a candidate for antitumor action.
Fructus Sophorae Enhances the Production of Prostaglandin E
and Tumor Necrosis Factor-α through Activation of MAPKs and PI3K/AKT Signaling Pathways in Murine Macrophages
Kang, Young-Soon ; Han, Min Ho ; Lee, Moon Hee ; Hong, Su Hyun ; Park, Heungsik ; Jung, Jae-Chul ; Lee, Jeongrai ; Lee, Eun-Woo ; Kang, Kyung Hwa ; Kim, Cheol Min ; Kim, Byung-Woo ; Choi, Yung Hyun ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1397~1403
DOI : 10.5352/JLS.2013.23.11.1397
Fructus Sophorae, the dried ripe fruit of Styphnolobium japonicum (L.), is an herbal ingredient used in traditional Oriental medicine. This study was carried out to investigate the effects of Fructus Sophorae extracts (FSE) on immune modulation in a murine RAW 264.7 macrophage model. As immune response parameters, the production of prostaglandin
) and tumor necrotic
) were evaluated. Our data revealed that FSE increased the macrophage activation and the production of
, which was consistently correlated with upregulation of cyclooxygenase-2 (COX-2) and
expression at both transcriptional and translational levels. On comparative cytokine protein array, FSE significantly increased several cytokines, which was associated with phosphorylation of mitogen- activated protein kinases (MAPKs), including extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun N-terminal kinase (JNK), and Akt in RAW 264.7 cells. However, each inhibitor of these molecules attenuated the FSE-induced
production. These results indicate that FSE activated macrophages through the activation of MAPKs and phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways in RAW 264.7 macrophages. These findings suggest that FSE may provide a promising source of an immunoenhancing agent.
Effects of (-)-Epigallocatechin-3-gallate on the Release of Pancreatic Enzymes and Expression of Regenerating Genes in Ethanol-injured Murine Pancreatic Primary Acinar Cells
Kim, Sung Ok ; Choi, Yung Hyun ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1404~1408
DOI : 10.5352/JLS.2013.23.11.1404
(-)-Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, has been shown to have strong antibacterial, antiviral, antioxidant, anti-inflammatory, and chemopreventive effects. However it is unknown whether EGCG can recover alcohol-associated pancreatitis. The aim of this study was to investigate the effects of EGCG on pancreatic enzyme activities and the expressions of pancreatic regenerating related markers, such as adenosine monophosphate-activated protein kinase (AMPK), raf-1 kinase inhibitor protein (RKIP), and Regenerating gene 1 (Reg1), in mice pancreatic primary acinar cells. Our results revealed that activities of
-amylase and chymotrypsin were significantly increased in the cells treated with ethanol compared to the untreated control cells; however, the increased activities of both enzymes were markedly reduced by pretreatment with EGCG. Phosphorylation of AMPK and total expression of RKIP were decreased in the ethanol-treated primary acinar cells; however, these were both significantly increased in the EGCG-pretreated cells. In addition, when EGCG was treated, expression of Reg1 was markedly increased compared with that of the control or the ethanol-treated primary acinar cells, demonstrating that EGCG can modulate pancreatic regenerating related genes. Therefore, our findings suggest that EGCG may have therapeutic utility in the prevention or treatment of alcohol-associated pancreatitis.
Functional Understating of Fibroblastic Reticular Cell within Lymph Node Stroma
So, Deuk Won ; Ryu, Sul Hwa ; Lee, Jong-Hwan ;
Journal of Life Science, volume 23, issue 11, 2013, Pages 1409~1414
DOI : 10.5352/JLS.2013.23.11.1409
Lymph node (LN) is the sites where mature lymphocytes become stimulated to respond to invading pathogens in the body. Lymphocytes screen the surfaces of pathogen-carrying antigen-presenting cells for cognate antigens, while moving along stromal structural back bone. Fibroblastic reticular cells (FRC) is stromal cell forming the 3 dimensional structure networks of the T cell rich zones in LN, and provide a guidance path for immigrating T lymphocytes. In these cooperative environments, the cell to cell bidirectional interactions between FRC and T cells in LN are therefore essential to the normal functioning of these tissues. Not only do FRCs physically construct LN architecture but they are essential for regulating T cell biology within these domains. FRC interact closely with T lymphocytes, is providing scaffolds, secreting soluble factors including cytokine in which FRCs influence T cell immune response. More recently, FRC have been found to induce peripheral T cell tolerance and regulate the extent to which newly activated T cells proliferate within LN. Thus, FRC-T cell crosstalk has important consequences for regulating immune cell function within LN. In addition, FRC have profound effects on innate immune response by secreting anti-microbial peptides and complement, etc in the inflammatory milieu. In summary, we propose a model in which FRC engage in a bidirectional touch to increase the T cell biological efficiency between FRC and T cells. This collaborative feedback loop may help to maintain tissue function during inflammation response.