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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 23, Issue 12 - Dec 2013
Volume 23, Issue 11 - Nov 2013
Volume 23, Issue 10 - Oct 2013
Volume 23, Issue 9 - Sep 2013
Volume 23, Issue 8 - Aug 2013
Volume 23, Issue 7 - Jul 2013
Volume 23, Issue 6 - Jun 2013
Volume 23, Issue 5 - May 2013
Volume 23, Issue 4 - Apr 2013
Volume 23, Issue 3 - Mar 2013
Volume 23, Issue 2 - Feb 2013
Volume 23, Issue 1 - Jan 2013
Selecting the target year
An Investigation of Glyceollin I's Inhibitory Effect on The Mammalian Adenylyl
Kim, Dong-Chan ; Kim, Nam Doo ; Kim, Sung In ; Jang, Chul-Soo ; Kweon, Chang Oh ; Kim, Byung Weon ; Ryu, Jae-Ki ; Kim, Hyun-Kyung ; Lee, Suk Jun ; Lee, Seungho ; Kim, Dongjin ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 609~615
DOI : 10.5352/JLS.2013.23.5.609
Glyceollin I has gained attention as a useful therapy for various dermatological diseases. However, the binding property of glyceollin I to the mammalian adenylyl cyclase (hereafter mAC), a critical target enzyme for the down-regulation of skin melanogenesis, has not been fully explored. To clarify the action mechanism between glyceollin I and mAC, we first investigated the molecular docking property of glyceollin I to mAC and compared with that of SQ22,536, a well-known mAC inhibitor, to mAC. Glyceollin I showed superiority by forming three hydrogen bonds with Asp 1018, Trp 1020, and Asn 1025, which exist in the catalytic site of mAC. However, SQ22,536 formed only two hydrogen bonds with Asp 1018 and Asn 1025. Secondly, we confirmed that glyceollin I effectively inhibits the formation of forskolin-induced cAMP and the phosphorylation of PKA from a cell-based assay. Long term treatment with glyceollin I had little effect on the cell viability. The findings of the present study also suggest that glyceollin I may be extended to be used as an effective inhibitor of hyperpigmentation.
Anti-aging and Anti-diabetes Effects of Aconitum pesudo-laeve var. erectum Extracts
Kim, Jeung-Hoan ; Lee, Soo-Yeon ; Kwon, O-Jun ; Park, Joo-Hoon ; Lee, Jin-Young ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 616~621
DOI : 10.5352/JLS.2013.23.5.616
Aconitum pesudo-laeve var erectum has been known to possess anti-inflammatory activity and modulate the intestinal immune system. In addition, it has traditionally been used for the treatment of water retention in the body. In this study, the anti-aging and anti-diabetes effects of water and ethanol extracts from Aconitum pesudo-laeve var. erectum were investigated. The activities of each extract were measured by antioxidant tests such as DPPH and ABTS radical scavenging activity, antioxidant protection factor (PF), TBARs content, and
-glucosidase inhibition activity assay. DPPH radical scavenging activity was found in over 50% of water and ethanol extracts at
, respectively. The ABTS radical scavenging activity of ethanol extract was
in water, which was highest among the ethanol extract concentrations. PFs measured with
-carotene-linoleate model systems were in the order of ethanol (1.49 PF at
) > ethanol (1.40 PF at
) > water (1.33 PF at
) > water (1.27 PF at
). TBARs content in ethanol extracts (
, which was lower than that of water extracts and other ethanol extract concentrations. The extracts also showed over 90% of
-amylase inhibition and over 60% of
-glucosidase inhibition ratio in water (
) and ethanol extracts (100~
). These results suggest that Aconitum pesudo-laeve var. erectum extracts could be used as a cosmetic source and preventive agent for aging and diabetes.
Induction of Mitotic Arrest and Apoptosis by Diallyl Trisulfide in U937 Human Leukemia Cells
Park, Hyun Soo ; Lee, Jun Hyuk ; Son, Byoung Yil ; Choi, Byung Tae ; Choi, Yung Hyun ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 622~628
DOI : 10.5352/JLS.2013.23.5.622
Diallyl trisulfide (DATS), one of the major organosulfur components of garlic (Allium sativum), has various biological effects such as anti-microbial and anti-cancer activities. However, the molecular mechanisms of growth inhibition related to cell cycle arrest are poorly understood. In this study, we investigated the effects of DATS on cell cycle progression in U937 human leukemia cells. Treatment with DATS in U937 cells resulted in inhibition of cell viability through G2/M arrest and apoptosis. DATS-induced G2/M arrest was associated with up-regulation of cyclin B1 and cyclin-dependent kinase 1 (CDK1). DATS also significantly increased levels of phospho-histone H3, which is a mitosis-specific marker, indicating that DATS induced mitotic arrest but not G2 arrest in U937 cells. DATS treatment also generated the reactive oxygen species (ROS) in U937 cells; however, pretreatment with N-acetyl-l-cysteine (NAC), a ROS scavenger, significantly attenuated DATS-induced mitotic arrest and apoptosis. Taken together, our data indicate that DATS exhibits anti-cancer effects through mitotic arrest and apoptosis in a ROS-dependent manner.
Anti-Inflammatory Effects of Picrasma Quassioides (D.DON) BENN Leaves Extracts
Jung, Yeon Seop ; Eun, Cheong Su ; Jung, Young Tae ; Kim, Hyun Jeong ; Yu, Mi Hee ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 629~636
DOI : 10.5352/JLS.2013.23.5.629
This study was performed to evaluate the anti-inflammatory and antioxidant activities of methanol extract from the leaves of Picrasma quassioides BENNET (PLME). The antioxidant effects of PLME were measured based on polyphenol and flavonoid contents. PLME was found to have
high polyphenol and flavonoid contents. Cell viability was determined by MTT assay. The production of nitric oxide (NO) and prostaglandin
) was measured by Griess assay and enzyme-linked immunosorbent assay (ELISA). In order to effectively anti-inflammatory agents, we examined the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO and
in RAW 264.7 cells. PLME significantly decreased the production of NO and
in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. In addition, PLME reduced the NF-
phosphorylation in RAW 264.7 cells upon stimulation with LPS (100 ng/ml) for 24 h. These results provide evidence for the anti-inflammatory and antioxidant effects of Picrasma quassioides leaves.
Evaluation of Natural Oils in Antimicrobial Activity and Rodent Repellent Effectiveness
Park, Sung Jin ; Yu, Mi Hee ; Lee, Eun Ji ; Jang, Soon Ho ; Lee, In Seon ; Kim, Bae Hwan ; Lee, Sam Pin ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 637~642
DOI : 10.5352/JLS.2013.23.5.637
This study was performed to evaluate the rodent repellency and antimicrobial activity of natural oils. Thirteen natural essential oils were evaluated for repellent efficacy against rodent feed intake suppression activity. Salvia sclarea (sage), Cinnamomum zeylanicum (cinnamon) Cymbopogon citratus (lemon grass), and Syzygium aromaticum (clove) showed the best repellent efficacy among tested natural oils. Antimicrobial activity against Gram-negative (four strains) and Gram-positive (four strains) bacteria were evaluated using four natural oils, which showed high efficacy on the rodent repellent test. Syzygium aromaticum showed antimicrobial activity against all tested bacteria and Cymbopogon citratus, Cinnamonum zeylanicum, and Salvia sclarea showed antimicrobial activity against some tested bacteria.
The Antioxidative Effect of Ethanol Extracts from Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium Linn, and Lonicera japonica
Jung, Min-Hwa ; Lee, Su-Seon ; Park, Si-Hyang ; Hwang, Hye-Jung ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 643~649
DOI : 10.5352/JLS.2013.23.5.643
In this study, we examined the antioxidative activities of ethanol extracts obtained from three plants; Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium Linn, and Lonicera japonica, which have traditionally been used as drugs in Eastern medicine in Korea. Their extraction yields were 7.01%, 2.92%, and 7.95% in Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium L, and Lonicera japonica, respectively. The contents of the phenolic compounds were
in Lithospermum erythrorhizon Siebold & Zucc., and Xanthium strumarium L, respectively. Furthermore, the radical scavenging activity measured through the DPPH assay appeared highest in the Lonicera japonica's extract, and its
was 0.24 mg/ml. Compared to the control, the xanthine oxidase inhibiting activities of all extracts were effective at 0.01 mg/ml concentration. Superoxide radical scavenging activity in Lithospermum erythrorhizon Siebold & Zucc. and Lonicera japonica was more than 80%, with a concentration of 50 mg/ml. OH radical scavenging activity was 40% in the three plants, with a concentration of 50 mg/ml scavenging activity. From our results, we demonstrated that the ethanol extracts of three medicinal plants have antioxidant activities and could be potential candidates for natural antioxidants.
Effects of Lycopene on Endothelial Protein C Receptor Shedding In Vitro and In Vivo
Yoo, Hayoung ; Lee, Hyun-Shik ; Lee, Wonhwa ; Bae, Jong-Sup ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 650~656
DOI : 10.5352/JLS.2013.23.5.650
Endothelial protein C receptor (EPCR) plays a pivotal role in augmenting Protein C activation through the thrombin-thrombomodulin complex. EPCR activity is markedly changed by ectodomain cleavage and released as the soluble protein (sEPCR). EPCR shedding is mediated by tumor necrosis factor-
converting enzyme (TACE). Lycopene found in tomatoes and tomato products has anti-oxidant, anti- cancer and anti-inflammatory effects. However, little is known about the effects of lycopene on EPCR shedding. We investigated this issue by monitoring the effects of lycopene on the phorbol-12-myristate 13-acetate (PMA), tumor necrosis factor (TNF)-
, interleukin (IL)-
and on the cecal ligation and puncture (CLP)-mediated EPCR shedding. Data showed that lycopene potently inhibited the PMA, TNF-
and CLP-induced EPCR shedding by suppressing TACE expression. Furthermore, lycopene reduced PMA-stimulated phosphorylation of p38, extracellular regulated kinases (ERK) 1/2 and c-Jun N-terminal kinase (JNK). Given these results, lycopene should be viewed as a candidate therapeutic agent for the treatment of various severe vascular inflammatory diseases via inhibition of the EPCR shedding.
Effects of Cheonggukjang Diet and Aerobic Exercise on Lipid Metabolism and Antioxidant Enzyme in Rats
Kim, Sang-Woo ; Jeong, Seon-Tea ; Baek, Yeong-Ho ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 657~663
DOI : 10.5352/JLS.2013.23.5.657
The purpose of this study was to examine the effects of a cheonggukjang diet and aerobic exercise on lipid metabolism and antioxidant enzyme activity in rats. Thirty-two Sprague Dawley rats were randomized into a cheonggukjang diet with aerobic exercise (A), aerobic exercise (B), cheonggukjang diet (C), and control group (D). The cheonggukjang diet group consumed 20 g of 20% cheonggukjang a day with their normal diet. Exercise training consisted of treadmill running (25~40 min, 5 day/wk) and the exercise intensity was gradually increased. The results are as follows: T-C was significantly lower (p<0.05) in A compared to B and C. TG was significantly lower (p<0.001) in A compared to D. B and C were significantly lower than D. HDL-C was significantly higher (p<0.05) in C compared to D. LDL-C was not statistically different across the groups. Additionally, TBARS were not statistically different in the control or experimental groups. SOD was significantly lower (p<0.05) in A compared to D. C was significantly lower (p<0.05) that of D. CAT and GPx failed to reach the statistical difference between experimental and control groups. The major findings of this study were that aerobic exercise with a chenggukjang diet intervention improved lipid profiles and antioxidant capacity in this animal model. Therefore, a cheonggukjang diet and aerobic exercise will help to improve antioxidant capacity and prevent lifestyle related diseases.
Analysis of General Composition and Harmful Material of Protaetia brevitarsis
Chung, Mi Yeon ; Gwon, Eun-Yeong ; Hwang, Jae-Sam ; Goo, Tae-Won ; Yun, Eun-Young ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 664~668
DOI : 10.5352/JLS.2013.23.5.664
To evaluate Protaetia brevitarsis as a food material, we investigated its composition of nutritional and harmful components. Analysis of nutritional composition (moisture, crude protein, crude fat, crude ash, crude fiber, and carbohydrates) showed that the content of crude protein, fat, fiber, and ash were
% in Protaetia brevitarsis powder, respectively. Amino acids were composed of 17.68% essential and 33.97% non-essential in Protaetia brevitarsis powder. Protaetia brevitarsis powder contained 61.10% unsaturated fatty acid with oleic acids. Additionally, Protaetia brevitarsis powder had a large quantity of minerals related to body organization, such as K (1597 mg/100 g), P (724.1 mg/100 g), Mg (366.3 mg/100 g), and so on. We also confirmed that all bacteria and all heavy metals analyzed in this study, except for very small amount of Hg (
mg/kg), were not detected in the lysophilized Protaetia brevitarsis powder.
Stability of Monascus Pigment Produced by Monascus sp. MK2
Jeon, Chun-Pyo ; Lee, Jung-Bok ; Kwon, Gi-Seok ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 669~675
DOI : 10.5352/JLS.2013.23.5.669
The stability of monascus pigment produced by Monascus sp. MK2 was investigated according to light, pH, temperature, organic acid, synthetic antioxidant, and natural substance. The light stability was increased (sun light < fluorescent lamp < ultraviolet rays < dark condition) when storing the monascus pigments. Although the monascus pigments were stable in the range of pH 3.0, the degradation rate of pigment was increased at pH 11.0. The stability of monascus pigment gradually decreased as temperature increased. In addition, pigment stability was increased by adding the synthetic antioxidant and natural substance.
Characterization of Perchlorate-Removal Using Elemental Sulfur Granules and Activated Sludge
Han, Kyoung-Rim ; Ahn, Yeonghee ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 676~681
DOI : 10.5352/JLS.2013.23.5.676
) is an emerging contaminant found in surface water and soil/groundwater. Microbial removal of perchlorate is the method of choice since perchlorate-reducing bacteria (PRB) can reduce perchlorate to harmless end-products. A previous study  showed experimental evidence of autotrophic perchlorate removal using elemental sulfur granules and activated sludge. The granular sulfur is a relatively inexpensive electron donor, and activated sludge is easily available from a wastewater treatment plant. A batch test was performed in this study to further investigate the effect of various environmental parameters on the perchlorate degradation by sludge microorganisms when elemental sulfur was used as electron donor. Results of the batch test suggest optimum conditions for autotrophic perchlorate degradation by sludge microorganisms. The results also show that sulfur-oxidizing PRB enriched from activated sludge removed perchlorate better than activated sludge. Taken together, this study suggests that autotrophic perchlorate removal using elemental sulfur and activated sludge can be improved by employing optimized environmental conditions and enrichment culture.
Effect of Fermented Cudrania tricuspidata Fruit Extracts on the Generation of the Cytokines in Mouse Spleen Cells
Seo, Min Jeong ; Kang, Byoung Won ; Park, Jeong Uck ; Kim, Min Jeong ; Lee, Hye Hyeon ; Kim, Nam Hee ; Kim, Kwang Hyuk ; Rhu, En Ju ; Jeong, Yong Kee ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 682~688
DOI : 10.5352/JLS.2013.23.5.682
We investigated a physiological function by fermenting a medicinal mushroom, (Cudrania tricuspidata fruit). A fermentation using lactic acid bacteria and the extracts isolated from 70% ethanol fractionation was included in cultured mouse spleen cells for cytokine secretion. As a result, total polyphenol content improved by 47% by organic acid fermentation. This was regarded as immune activity in fermented C. tricuspidata fruits, as the levels of interleukin (IL)-2 and IL-4 secretion increased. In addition, when the extracts were treated with a stimulant lipopolysaccharide, the secretion of helper T (Th) 1 cytokines IL-2, IL-12, and tumor necrosis factor-
was suppressed, while the secretion of Th2 cytokines IL-4, IL-5, IL-6, and IL-10 significantly increased. Therefore, this study suggests that fermentative C. tricuspidata fruit extracts can contribute to the suppression of cellular immune reactions induced by the expression of Th1 cells and activation of the expression of Th2 cells inducing humoral immune reactions associated with the antibody generation by B lymphocytes.
Cytotoxic Mechanism of Docosahexaenoic Acid in Human Oral Cancer Cells
Hong, Tae-Hwa ; Kim, Hoon ; Shin, Soyeon ; Jing, Kaipeng ; Jeong, Soyeon ; Lim, Hyun ; Yun, Donghyuk ; Jeong, Ki-Eun ; Lee, Myung-Ryul ; Park, Jong-Il ; Kweon, Gi-Ryang ; Park, Seung Kiel ; Hwang, Byung-Doo ; Lim, Kyu ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 689~697
DOI : 10.5352/JLS.2013.23.5.689
In the United States, about 40,000 new cases of oral cancer are diagnosed each year and nearly 7,800 patients died from it in 2012. Omega-3 polyunsaturated fatty acids have been found to have anticancer effects in a variety of cancer cell lines and animal models, but their effect in oral cancer remains unclear. This study was designed to examine the effect of docosahexaenoic acid (DHA, a kind of omega-3 fatty acid) on oral cancer cells and the molecular mechanism of its action. We found that exposure of squamous cell carcinoma-4 (SCC-4) and squamous cell carcinoma-9 (SCC-9) human oral cancer cells to DHA induced growth inhibition in a dose- and time-dependent manner. Meanwhile, in addition to the elevated levels of apoptotic markers, such as cleaved PARP, subG1 portion and TUNEL-positive nuclei, DHA led to autophagic vesicle formation and an increase in autophagic flux, indicating the involvement of both apoptosis and autophagy in the inhibitory effects of DHA on oral cancer cells. Further experiments revealed that the apoptosis and autophagy induced by DHA were linked to inhibition of mammalian target of rapamycin (mTOR) signaling by AKT inhibition and AMP-activated protein kinase (AMPK) activation in SCC-9 cells. Together, our results suggest that DHA induces apoptosis- and autophagy-associated cell death through the AMPK/AKT/mTOR signaling pathway in oral cancer cells. Thus, utilization of omega-3 fatty acids may represent a promising therapeutic approach for chemoprevention and treatment of human oral cancer.
Effect of Feeding with High γ-Aminobutyric Acid (GABA) Containing Giant Embryo Black Sticky Rice (Oryza sativa L.) on Alcohol Intake in C57BL/6 Mice
Kim, Hyeun-Kyeung ; Kim, Sung-Gon ; Lee, Jin-Sung ; Lee, Sang-Shin ; Jung, Woo-Young ; Han, Sang-Ik ; Kim, Byung-Ju ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 698~702
DOI : 10.5352/JLS.2013.23.5.698
It is known that black sticky rice with giant embryo (BSRGB, Milyang 263) and giant embryo rice (GER) contains higher levels of GABA than rice. Therefore, feeding BSRGB, GER, or rice freely to C57BL/6 mice with two-hour alcohol intake for 16 days was investigated. For two-hour alcohol intake, a repeated measure ANOVA (three treatment groups repeated across 16 days; 8 two-day blocks) yielded a significant group by block interaction (df=16, F=3.109, p=0.004). The independent t-test showed that significant suppression of two-hour alcohol intake was observed when subjects were administered with BSRGB, compared with the rice alone across all the two-day blocks (p<0.05). The paired t-test revealed that a significant suppression of two-hour alcohol intake was observed starting 4 to 16 days after freely feeding with BSRGB compared to before feeding. However, there is no significant difference in the two-hour alcohol intake observed between the before and after administration of rice. A repeated measure ANOVA revealed no significant group by block interaction for 22-hour water intake and body weight. However, a repeated measure ANOVA revealed a significant grouping by block interaction for food intake. These results indicate a change of two-hour alcohol intake is presumably caused by GABA, which is found in higher levels in BSRGB than in rice.
Prevalence of Extended-spectrum β-Lactamase and Quinolone Resistance Genes in Escherichia coli Clinical Isolates and their Antibiotic Resistance
Lee, Min Hyeok ; Hwang, Yeoung Min ; Baik, Keun Sik ; Cho, Hyun Wook ; Seong, Chi Nam ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 703~709
DOI : 10.5352/JLS.2013.23.5.703
The aim of this study was to investigate the prevalence of Extended-spectrum
-lactamase (ESBL) gene and quinolone resistance determinant (qnr) and the pattern of antibiotic resistance in the ESBL-producing Escherichia coli clinical isolates. The 42 ESBL-producing strains from total 274 isolates were detected using a double disk synergy test. They were isolated from various specimens, such as urine (28 strains), sputum (6 strains), pus (3 strains), wound (2 strains), blood (2 strains), and tissue (1 strain). Using the PCR with the specific primers ESBL, ESBL and qnr gene types were determined. Thirty-five strains possessed one or two ESBL genes. CTX-M-1 type was the most abundant followed by CTX-M-9 type and TEM, but SHV, CTX-M-2, and CTX-M-8 gene types were not detected. qnr gene types were detected from ten isolates in the order of qnrB4, qnrB1, and qnrS. Coexistence of ESBL and qnr genes was found. ESBL-producing isolates showed high resistance against some antibiotics, such as cefotaxmie (80.0%), levofloxacin (82.9%), and ampicillin (100%). Neither a synergy effect from the coexistence of ESBL and qnr genes on antibiotic resistance nor a correlation between the production of qnr gene and quinolone resistance were found.
Potential Role of Hedgehog Signaling in Radiation-induced Liver Fibrosis
Wang, Sihyung ; Jung, Youngmi ;
Journal of Life Science, volume 23, issue 5, 2013, Pages 710~720
DOI : 10.5352/JLS.2013.23.5.710
Radiotherapy is commonly used in treating many kinds of cancers which cannot be cured by other therapeutic strategies. However, radiotherapy also induces the damages on the normal tissues. Radiation-induced fibrosis is frequently observed in the patients undergoing radiotherapy, and becomes a major obstacle in the treatment of intrahepatic cancer. Hedgehog (Hh) that is an essential in the liver formation during embryogenesis is not detected in the healthy liver, but activated and modulates the repair process in damaged livers in adult. The expression of Hh increases with the degree of liver damage, regulating the proliferation of hepatic progenitors and hepatic stellate cells (HSC). In addition, Hh induces epithelial-to-mesencymal transition (EMT) and activation of myofibroblasts. In the irradiated livers, up-regulated expression of Hh signaling was associated with proliferation of progenitors, EMT induction, and increased fibrosis. Female-specific expression of Hh leaded to the expansion of progenitors and the accumulation of collagen in the irradiated livers of female mice, indicating that gender disparity in Hh expression may be related with radiation-susceptibility in female. Hence, Hh signaling becomes a novel object of studies for fibrogenesis induced by radiation. However, the absence of the established experimental animal models showing the similar physiopathology with human liver diseases and fibrosis-favorable microenvironment hamper the studies for the radiation-induced fibrosis, providing a few descriptive results. Therefore, further research on the association of Hh with radiation-induced fibrosis can identify the cell and tissue-specific effects of Hh and provides the basic knowledge for underlying mechanisms, contributing to developing therapies for preventing the radiation-induced fibrosis.