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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Korean Society of Life Science
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Volume & Issues
Volume 25, Issue 12 - Dec 2015
Volume 25, Issue 11 - Nov 2015
Volume 25, Issue 10 - Oct 2015
Volume 25, Issue 9 - Sep 2015
Volume 25, Issue 8 - Aug 2015
Volume 25, Issue 7 - Jul 2015
Volume 25, Issue 6 - Jun 2015
Volume 25, Issue 5 - May 2015
Volume 25, Issue 4 - Apr 2015
Volume 25, Issue 3 - Mar 2015
Volume 25, Issue 2 - Feb 2015
Volume 25, Issue 1 - Jan 2015
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Inhibition and Chemical Mechanism of Protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707
Kang, Taekyeong ; Kim, Sang Ho ; Jung, Mi Ja ; Cho, Yong Kweon ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 487~495
DOI : 10.5352/JLS.2015.25.5.487
We carried out pH stability, chemical inhibition, chemical modification, and pH-dependent kinetic parameter assessments to further characterize protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707. Protocatechuate 3,4-dioxygenase was stable in the pH range of 4.5~10.5. L-ascorbate and glutathione were competitive inhibitors with
values of 0.17 mM and 0.86 mM, respectively. DL-dithiothreitol was a noncompetitive inhibitor with a
value of 1.57 mM and a
value of 8.08 mM. Potassium cyanide, p-hydroxybenzoate, and sodium azide showed a noncompetitive inhibition pattern with
values of 55.7 mM, 0.22 mM, and 15.64 mM, and
values of 94.1 mM, 8.08 mM, and 662.64 mM, respectively.
was the best competitive inhibitor with a
were also competitive inhibitors with
values of 1.21 mM, 0.85 mM, 3.98 mM, and 0.21 mM, respectively. Other metal ions showed noncompetitive inhibition patterns. The pH-dependent kinetic parameter data showed that there may be at least two catalytic groups with pK values of 6.2 and 9.4 and two binding groups with pK values of 5.5 and 9.0. Lysine, cysteine, tyrosine, carboxyl, and histidine were modified by their own specific chemical modifiers, indicating that they are involved in substrate binding and catalysis.
The Effects of Temperature on Maintaining the Stability of Water Quality in Biofloc-based Zero-water Exchange Culture Tanks
Cho, Seo-Hyun ; Jeong, Jong-Heon ; Kim, Myung-Hee ; Lee, Kyu-Tae ; Kim, Dae-Jung ; Kim, Kwang-Hyun ; Oh, Sang-Pil ; Han, Chang-Hee ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 496~506
DOI : 10.5352/JLS.2015.25.5.496
This study explored adequate water temperature ranges for maintaining stable water quality in a biofloc- based zero-water exchange culture system. Five experimental tanks with the following temperatures were set up: 10℃, 15℃, 20℃, 25℃, and 30℃. First, a biofloc-based culture system was developed in the experimental tanks; then, the tanks were stocked with goldfish and went without a water exchange for 60 days. Conditions for developing a biofloc-based culture system and stable water quality in low concentrations of inorganic nitrogen compounds at 10℃, 15℃, 20℃, 25℃, and 30℃ were maintained after 17, 26, 43, 68, and 78 days, respectively. Beginning from when the goldfish were stocked in the biofloc-based culture tanks, concentrations of
remained constant and at low levels at 10℃ and 15℃, but they showed a gradual increase at 20℃, 25℃, and 30℃. Concentrations of
at 10℃ and 15℃ did not remain at low levels and immediately increased. While
concentrations at above 20℃ remained constant and stable at relatively low levels,
concentrations showed a gradual increase. Conditions of 15℃ and below could not maintain low and stable concentrations of
. In the pH range of 4.0 to 6.0,
concentration decreased as the pH rose. However, there was no correlation between pH and
concentration in the pH range of 6.0 to 8.0. These results indicate that pH levels should be kept at pH 6.0 and above to maintain a low and stable concentration of
at above 20℃.
Cloning and Characterization of D-xylulose Kinase from Kocuria gwangalliensis Strain SJ2
Jeong, Tae Hyug ; Hwang, Tae Kyung ; Seo, Yong Bae ; Kim, Young Tae ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 507~514
DOI : 10.5352/JLS.2015.25.5.507
D-Xylulose is phosphorylated to D-xylulose-5-phosphate by D-xylulose kinase before it enters glycolysis via the nonoxidative pentose phosphate pathway. A gene encoding a novel D-xylulose kinase (XK) from K. gwangalliensis strain SJ2 was sequenced and expressed in E. coli. The sequence of the isolated XK gene was 1,419 bp, encoding 472 amino acids. The XK protein was more closely related to the Arthrobacter phenanthrenivorans XK than to the Bifidobacterium catenulatum one, as reflected in the sequence identity (54.9% vs. 38.7%). The XK gene was subcloned into the pCold-II expression vector. The resulting plasmid was transformed into E. coli strain BL21 (DE3) cells and the expression of the recombinant XK protein was induced by the addition of IPTG. The resulting protein was expressed as a fusion protein of approximately 48 kDa containing a N-terminal six-histidine extension that was derived from the expression vector. The expressed protein was homogenized by affinity chromatography and showed enzymatic activity corresponding to D-xylulose kinase. XK enzyme kinetic studies with D-xylulose and ATP showed a Km of 250±20 μM and 1,300±50 μM, respectively. The results obtained from this study will provide a wider knowledge base for the characterization of D-xylulose kinase at the molecular level.
Anti-oxidative and Anti-cancer Activities of Treculia africana Extract in Human Colon Adenocarcinoma HT29 Cells
Oh, You Na ; Jin, Soojung ; Park, Hyun-jin ; Kim, Byung Woo ; Kwon, Hyun Ju ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 515~522
DOI : 10.5352/JLS.2015.25.5.515
Treculia africana Decne, a breadfruit species, is native to many parts of West and Tropical Africa. The breadfruit belongs to the family Moraceae and is one of the four members of the genera Treculia. The crude extract of T. africana has been used in folk medicine as an anti-inflammatory agent for various ailments, such as whooping cough. In this study, we evaluated the anti-oxidative and anti-cancer activities of the methanol extract of T. africana Decne (META) and the molecular mechanisms of its anti-cancer effects in human colon carcinoma HT29 cells. The META exhibited anti-oxidative activity through a DPPH radical scavenging capacity and inhibited cell growth in a dose-dependent manner in HT29 cells. META treatment induced apoptosis of HT29 cells, showing an increase in the percentage of both SubG1 cells and Annexin V-positive cells and the formation of apoptotic bodies in a dose-dependent manner. META-mediated apoptosis was associated with the up-regulation of the death receptor FAS and Bax and a decrease in the Bcl-2 expression. META-treated HT29 cells also showed the release of cytochrome c from the mitochondria into the cytosol, activation of caspase-3, caspase-8, and caspase-9, and proteolytic cleavage of poly ADP-ribose polymerase (PARP). These findings suggest META may exert an anti-cancer effect in HT29 cells by inducing apoptosis through both intrinsic and extrinsic pathways.
Therapeutic Effect of Hydrocolloid Membrane Containing Liriope platyphylla Extracts on the Burn Wounds of SD Rats
Lee, Eun Hae ; Go, Jun ; Kim, Ji Eun ; Koh, Eun Kyoung ; Song, Sung Hwa ; Sung, Ji Eun ; Park, Chan Kyu ; Lee, Hyeon Ah ; Hwang, Dae Youn ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 523~532
DOI : 10.5352/JLS.2015.25.5.523
A variety of previous pharmacological studies have suggested Liriope platyphylla (L. platyphylla) may exert beneficial biological effects on inflammation, diabetes, neurodegenerative disorder, obesity, constipation, and atopic dermatitis. In addition, hydrocolloid membranes (HCMs) have attracted attention in dermatological care, including in the treatment of scleroderma skin ulcers, cutaneous ulcers, permanent tympanic membrane perforations, pressure sores, and decubitus ulcers in the elderly. To investigate the therapeutic effects of HCM containing an aqueous extract of L. platyphylla (HCM-LP) on second-degree burn wounds, their physico-chemical properties were analyzed and the therapeutic effects were observed in SD rats after treatment with HCM-LP for 14 days. Significant declines in tensile strength (38.4%) and absorptiveness (46.3%), as well as an increase in surface roughness (38.1%) were detected in HCM-LP compared with that of HCM. In SD rats with burned skin, the wound diameter was shorter in the HCM-LP treated group than in the GZ group on post-surgical day 14, while the significant improvements in scar tissue reduction, epithelium regeneration, angiogenesis, and extracellular matrix deposition were observed in the HCM-LP-treated group during all experimental periods. Overall, these results suggest HCM-LP may accelerate the process of healing the burn injury skin of SD rats through the regulation of angiogenesis and connective tissue formation.
Density Effect and Diversity of Fish in Water System at Both Reservoirs in the Youngsan-ri, Goseong-gun
Huh, Man Kyu ; Choi, Byoung-Ki ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 533~538
DOI : 10.5352/JLS.2015.25.5.533
Four sites and one site were used to analyze fish diversity at the water systems of the Sineun reservoir and the Jeonchon reservoir, respectively. The field experiments were conducted to test the density dependence that could lead to population regulation by artificial inferences and environmental changes. We examined the effects of environmental factors on fish densities using SMATR freeware. It was estimated to be reduced to the density effect at four sites in 2012. Shannon-Weaver indices of the diversity (H’) of the Sineun reservoir were similar to those of the Jeonchon reservoir. Species diversity was in a range of 0.645 to 2.105. The H’ value of the upper region was higher than those of middle and low regions were, and values of richness were lower in downstream than upstream. Using the maximum likelihood solution for the removal estimators of two low regions of the river stations, the estimated migration probabilities from the resident fish to the migrated fish for five species (Cyprinus cuvieri, Carassius auratus, Pseudorasbora parva, Misgurnus mizolepis, and Oryzias latipes) had a mean of 0.623. Especially, migration probabilities from the Jeonchon reservoir to the Sineun reservoir for five species were high (a mean of 0.681). The period of migration was suggested to be about one month because of short geographical distances (50 m). We found no significant difference between the three categories in the distribution of the other four species, indicating the species probability was similar among stations.
Protective Effect of Semisulcospira libertina Extract on Induced Hepatitis in Rats
Park, Young Mi ; Lim, Jae Hwan ; Lee, Jong Eun ; Seo, Eul Won ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 539~547
DOI : 10.5352/JLS.2015.25.5.539
This study aimed to investigate the protective effect of Semisulcospira libertina extract on liver injury induced by D-galactosamine in rats. After the administration of S. libertina extract, the local fat degeneration and infiltration of inflammatory cells in liver tissues were significantly decreased and peripheral hemorrhages around portal triads and central necrosis around central veins were found to be protective. The elevated levels of plasma ALT, AST, and LDH, the ALP activation lipid peroxidation, and the lipid contents of a damaged liver were recovered in experimental rats administrated with S. libertina extract, suggesting its role in blood enzyme activation and lipid content restoration within damaged rat liver tissues. Moreover, the expression rate of TNF-α, which accelerates inflammation and induces tissue damage and necrosis, was significantly decreased. In addition, the activities of antioxidant enzymes were more effectively upregulated compared to those of the control group induced hepatotoxicity. All data showed that S. libertina extract has a preventive role against liver damages, such as inflammation and tissue necrosis, as instigated with D-galactosamine by improving the activities of blood enzymes and antioxidant enzymes and modulating the expression of inflammation factor, suggest S. libertina extract is an effective medicinal resource for the restoration of hepatotoxicity.
Influence of Dietary Conjugated Linoleic Acid (CLA) and Carotenoids on Growth, Fatty Acid Composition, and 3T3-L1 Cells in Black Seabream (Acanthopagrus schlegeli)
Guo, Rui ; Rohmah, Zuliyati ; Choi, Kwang-Soo ; Park, Si-Hyang ; Ha, Yeong-Lae ; Kang, Seok-Joong ; Choi, Byeong-Dae ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 548~556
DOI : 10.5352/JLS.2015.25.5.548
Three groups of black seabream (Acanthopagrus schlegeli) were fed with treatment diets containing certain concentrations of conjugated linoleic acid (CLA) and carotenoids. The control group feed contained 0% CLA and 0% carotenoids, the CP10 group feed contained 1% CLA and 0.1% carotenoids, and the CP25 group feed contained 2.5% CLA and 0.1% carotenoids. The CP10 and CP25 groups demonstrated the enhanced growth and increased feed conversion efficiency of black seabream. The specific growth rates (SGRs) were 0.74, 0.81, and 0.97, while the feed conversion ratios (FCRs) were 2.65, 2.46, and 2.04 for the control, CP10, and CP25 groups, respectively. The total contents of high unsaturated fatty acid (HUFA) for the control, CP10, and CP25 groups were 41.0%, 41.7%, and 43.5%, respectively. CLA was deposited to the extent of 2.8% and 5.6% in the muscle, and 4.0% and 8.3% in the viscera of the CP10 and CP25 groups, respectively. Meanwhile, treatment with the viscera lipid extract (VLE) from CP25 fish evidently lowered 3T3-L1 adipocytes viability. The lipid extract from the muscle and viscera of black seabream contained ample amounts of beneficial substances, such as CLA, carotenoids, EPA, and DHA. CLA, which enriched black seabream muscle, could be categorized as a functional food and serve as a well-being food. Meanwhile, the fish oil from its viscera could serve as a high function supplement.
Quality Characteristics of Dolsan Leaf Mustard Kimchi Added with Seasoning of Sea tangle and Lentinus edodes
Oh, Sun-Kyung ; Kim, Ki-Woong ; Park, Wook-Min ; Kim, Nam-Hee ; Bae, Sang-Ok ; Choi, Myeong-Rak ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 557~567
DOI : 10.5352/JLS.2015.25.5.557
To increase the marketability of Dolsan leaf mustard Kimchi, seasonings were prepared by sea tangle and Lentinus edodes. Gat Kimchi A (GK-A) and Gat Kimchi B (GK-B) were prepared by addition of complex seasoning with Sea tangle (CSS), complex seasoning with Lentinus edodes (CSL), respectively and evaluated the quality characteristics. GK-A contained high minerals and dietary fiber and showed the effect of delaying pH-lowering and acidity-rising. There were no significant differences in salinity test after 70 days. Reducing sugar of test groups was higher than control. During the fermentation, Leuconostoc sp. was grown in GK-A and GK-B than control. Growth of control's Lactobacillus sp. increased 20 days after. Up to 30 days, lightness of control was high. Yellowness were increased browning potential in GK-B, 20 days after. From the initial to 10 days, Hardness of GK-A showed higher than the others. glutamic acid and aspartic acid content of GK-A was higher significantly than the others in the entire fermentation period (p<0.05). At 0, 10, 30 days, sweet-tasty amino acids of GK-A showed a significantly higher level than the others (p<0.05). From 10 days to 40 days of fermentation, the sensory evaluation of GK-A is better than the other groups. Comprehensively reviewed the above results, the conclusion is as follows: CSS made with sea tangle as a main ingredient. GK-A by seasoning the CSS to 90 g GK-A is expected as a new Dolsan leaf mustard products that can provide flavor and nutrition to the consumer at the same time.
Growth Promotion of Pavlova viridis by Bacteria Isolated from the Microalga
Ahamed, Sarker Anowarul Kabir ; Kim, Jin-Joo ; Choi, Tae-O ; Choi, Tae-Jin ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 568~576
DOI : 10.5352/JLS.2015.25.5.568
The marine microalga Pavlova viridis can grow fast and has the ability to accumulate essential nutrients for culturing marine animals, such as EPA and DHA, and it has been used as food for raring larval fish and prawn. The symbiotic relationship between the flagellate microalga Pavlova viridis and its associated bacteria was investigated. An axenic culture of P. viridis was obtained by repeated treatment of the microalga with an antibiotic cocktail. The axenic status was confirmed after sub-culturing three times in a sterile f/2 medium without an antibiotic. The axenic alga was then co-inoculated with five bacteria, arbitrarily designated as I1–I5, isolated from the alga to test the growth promotion of the algae. All bacterial strains promoted the growth of P. viridis, and bacterial isolate I3 was the most effective among the five bacteria tested. The cell number of P. viridis in the co-culture with I3 was significantly higher than that of the control culture. A sequence analysis of the 16S rRNA gene isolated from I3 revealed a 97% nucleotide sequence similarity to that of Citrobacter sp. The growth of strain I3 was also significantly enhanced by co-culturing with P. viridis, indicating a symbiotic relationship between the microalga and its associated bacterium. The association between the microalga and bacterium was confirmed by scanning electron microscopy.
Antioxidant Activity and Chemical Characteristics of Orostachys malacophyllus and Fermented Orostachys malacophyllus
Ahn, Hee-Young ; Choe, Da-Jeong ; Cho, Young-Su ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 577~584
DOI : 10.5352/JLS.2015.25.5.577
Orostachys malacophyllus grow on the old roofing tile or on the rock of mountain and is belong to Crassulaceae family. After air drying for Orostachys malacophyllus (OM), using the mixture of lactic acid bacteria (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus) was fermented (FOM). OM and FOM extracted using water (W), ethanol (E) and methanol (M) and were measured extracts yield, pH and Brix. Extracted OM and FOM were tested by in vitro experimental models of α,α´-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity, Fe/Cu reducing power, linoleic acid peroxidation using ferric thiocyanate and thiobarbituric acid (TBA) methods and peroxidation of rat liver homogenate. In addition, the bioactive materials (phenolic compounds, flavonoids and minerals) were measured. The highest phenolic compounds and flavonoids were OME 122.2 mg/100 g and OME 84.0 mg/100 g. OM and FOM′s major minerals were K, Ca and Mg. The highest free radical scavenging activity showed in FOMM (93.9%), OMM (93.4%), FOME (92.1%) and OME (91.9%) at 0.5% additional level. Fe reducing powers were strong in FOME and FOMM and Cu reducing powers were strong in OME and FOMM. Antioxidant activities on lipid peroxidation using rat homogenate as measured by TBARS method showed strong in FOME and on lipid peroxidation of linoleic acid as measured by ferric TBA method showed strong in OME and FOME and measured by ferric thiocyanate showed strong in FOME and FOMM.
Alteration of Stress Fiber in Fibroblastic Reticular Cells via Lymphotoxin β Receptor Stimulation is Associated with Myosin
Kim, Min Hwan ; Kim, Yeon Hee ; Choi, Woobong ; Lee, Jong-Hwan ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 585~593
DOI : 10.5352/JLS.2015.25.5.585
Stress fiber (SF) alteration is mediated by cellular receptors, which, upon interaction with the extracellular counterpart, signal to the actin cytoskeleton for remodeling. This association is mediated by a variety of scaffold and signaling factors, which control the mechanical and signaling activities of the interaction site. The heterotrimeric transmembrane lymphotoxin α1β2 (LTα1β2), a member of the tumor necrosis factor (TNF) family of cytokines, including soluble homotrimeric lymphotoxin (LT α), plays an important role in lymphoid tissue architecture. Ligation between LTα1β2 and the lymphotoxin β receptor (LTβR) activates signal-cascade in fibroblastic reticular cells (FRCs). We found LTβR stimulation using an agonistic anti-LTβR antibody alone or combined with LTα or TNFα induced changes in the actin and plasticity of cells. To clarify the involvement of myosin underlying the alteration, we analyzed the effect of myosin light chain kinase (MLCK) with an MLCK inhibitor (ML7), the phosphorylation level of myosin light chains (MLC), and the level of phospho-myosin phosphatase target subunit 1 (MYPT1) after treatment with an agonistic anti-LTβR antibody for cytoskeleton reorganization in FRCs. The inhibition of MLCK activity induced changes in the actin cytoskeleton organization and cell morphology in FRC. In addition, we showed the phosphorylation of MLC and MYPT1 was reduced by LTβR stimulation in cells. A DNA chip revealed the LTβR stimulation of FRC down-regulated transcripts of myosin and actin components. Collectively, these results suggest LTβR stimulation is linked to myosin regarding SF alteration in FRC.
Muskelin Interacts with Multi-PDZ Domain Protein 1 (MUPP1) through the PDZ Domain
Jang, Won Hee ; Jeong, Young Joo ; Choi, Sun Hee ; Lee, Won Hee ; Kim, Mooseong ; Kim, Sang-Jin ; Urm, Sang-Hwa ; Moon, Il Soo ; Seog, Dae-Hyun ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 594~600
DOI : 10.5352/JLS.2015.25.5.594
Protein-protein interactions have a critical role in the regulation of many cellular functions. Postsynaptic density-95/disks large/zonula occludens-1 (PDZ) domain is one of domains that mediate protein-protein interactions. PDZ domains typically bind to the specific motif at the carboxyl (C)-terminal end of partner proteins. Multi-PDZ domain protein 1 (MUPP1), which has 13 PDZ domains, serves a scaffolding function for structure proteins and signaling proteins, but the cellular function of MUPP1 has not been fully elucidated. We used the yeast two-hybrid system to identify proteins that interact with PDZ domains of MUPP1. We found an interaction between MUPP1 and muskelin. Muskelin was recently identified as a GABA
R) α1 subunit binding protein and known to have a role in receptor endocytosis and degradation. Muskelin bound to the 3
PDZ domain, but not to other PDZ domains of MUPP1. The C-terminal end of muskelin was essential for the interaction with MUPP1 in the yeast two-hybrid assay. When co-expressed in HEK-293T cells, muskelin but not the C-terminal deleted muskelin was co-immunoprecipitated with MUPP1. In addition, MUPP1 co-localized with muskelin at the same subcellular region in cells. These findings collectively suggest that MUPP1 or its interacting proteins could modulate GABA
R trafficking and turnover through the interaction with muskelin.
The Paradox of the Plankton
Lee, Hak Young ; Moon, Sung-Gi ; Huh, Man-Kyu ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 601~606
DOI : 10.5352/JLS.2015.25.5.601
Hutchinson (1961) proposed that the large number of species in most plankton communities is remarkable in review of the competitive exclusion principle, which suggests that in homogeneous, well-mixed environments species that compete for the same resources cannot coexist. The principle of competitive exclusion would lead us to conclude that only a few species could coexist in such circumstances. Nevertheless, numerous competing species in most natural habitats are able to coexist, while generally only few resources (niches) limit these communities. It is coined “the paradox of plankton” by Hutchinson. We reviewed some literature of the proposed solutions and give a brief overview of the mechanisms proposed so far. The proposed mechanisms that we discuss mainly include spatial and temporal heterogeneity in physical and biological environment, externally imposed or self-generated spatial segregation, horizontal mesoscale turbulence of ocean characterized by coherent vortices, oscillation and chaos generated by several internal and external causes, stable coexistence and compensatory dynamic under fluctuating temperature in resource competition, and finally the role of toxin-producing phytoplankton in maintaining the coexistence and biodiversity of the overall plankton populations. Especially we sited Roy and Chattopadhyay’s reviews and their toxin-producing hypothesis by phytoplankton. This review may be some information to study plankton communities and effect to put the solutions to the paradox that have been proposed over the years into perspective.
Anti-cancer Properties and Relevant Mechanisms of Cordycepin, an Active Ingredient of the Insect Fungus Cordyceps spp.,
Jeong, Jin-Woo ; Choi, Yung Hyun ;
Journal of Life Science, volume 25, issue 5, 2015, Pages 607~614
DOI : 10.5352/JLS.2015.25.5.607
Cancers are the largest cause of mortality and morbidity all over the world. Cordycepin, an adenosine analog, is a major functional component of the Cordyceps species, which has been widely used in traditional Oriental medicine. Over the last decade, this compound has been reported to possess many pharmacological properties, such as an ability to enhance immune function, as well as anti-inflammatory, antioxidant and anti-cancer effects. Recently, numerous studies have reported interesting properties of cordycepin as a chemopreventive agent as well. There is an accumulating body of experimental evidences suggesting that cordycepin impedes cancer progression by promoting apoptosis, inducing cell cycle arrest, modulating intracellular signaling pathways, and inhibiting invasion and metastasis of cancer cells. In many cancer cell lines, cordycepin inhibits growth and cell cycle progression by inducing arrest of the G2/M phase, resulting from the inhibition of retinoblastoma protein phosphorylation and induction of cyclin-dependent kinase inhibitors. To induce apoptosis, cordycepin activates the extrinsic and intrinsic pathways, which promotes reactive oxygen species generation and the downstream activation of kinase cascades. Cordycepin also can activate alternative pathways to cell death such autophagy. In addition, cordycepin can inhibit the pro-metastatic processes of cancer cell detachment, migration, and invasion through a variety of mechanisms, including the nuclear factor-kappa B and activated protein-1 signaling pathways. In this review, we summarized the variety of action mechanisms by which cordycepin may mediate chemopreventive effects on cancer and discussed the potential of this natural product as a promising therapeutic inhibitor of cancer development.