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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 8, Issue 6 - Dec 1998
Volume 8, Issue 5 - Oct 1998
Volume 8, Issue 4 - Aug 1998
Volume 8, Issue 3 - Jun 1998
Volume 8, Issue 2 - Apr 1998
Volume 8, Issue 1 - Feb 1998
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Somatic Embryogenesis and Plant Regeneration from Poinsettia (Euphorbia pulcherrima L.) Stem Explants
Hee-Sung Park ;
Journal of Life Science, volume 8, issue 6, 1998, Pages 623~626
Conditions for somatic embryogenesis and plant regeneration from stem tissues of Euphorbia pulcherrima were esta-blished. Explants from leaf, petiole, stem were examined for their embryogenesis on MS solid medium supplemented with plant growth hormones in combination at various concentrations. From leaf or petiole explants, callus was indu-ced well but never proceeded to the embryonic stage in our expermental conditions. From stem explants, however, multiple shoots following callus induction emerged in about 6 to 8 weeks on MS agar medium supplemented with 1.5 mg/L of benzyladenine. Upon transfer, roots were developed on hormone-free MS solid medium.
Preparation of an Immobilized Enzyme for Enhancing Thermostability of the Crude Proteinase from Fish Intestine
Journal of Life Science, volume 8, issue 6, 1998, Pages 627~637
In order to utilize tuna pyloric caeca among fish intestines wasted when treated raw fish in fish processing manufactory, a crude enzyme with high proteolytic activity was extracted and its optimum condition were investigated. An immobilized enzymes also were prepared by adsorption method to enhance thermostability of the crude proteinase. The yield of the crude proteinase was approximately 2.7% on dry basis. The proteolytic activity for casein was 0.54 U/mg protein, for BTEE 1.10 U/mg protein, and for BAEE 2.69 U/mg protein. It was almost similar to that of the commercial trypsin purified. Optimum hydrolysis activity of the crude proteinase was about 80%, as the degree of hydrolysis for casein, at pH 10.0 and 45
for 12 hrs. Also, when the crude proteinase was immobilized on DEAE-Cellulose and chitin, the residual activities remained after 7 days of pre-incubation time were maintained about 90% or more and their thermostabilities were enhanced by about 50%, compared with the native enzyme.
Translocation of Protein Kinase C Isozymes in the Breast Cancer Cell Line
Won Chul Choi ; Joo Young Son ; Seok Jin Seo ;
Journal of Life Science, volume 8, issue 6, 1998, Pages 638~647
Protein Kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA), bryostatin, and dioctanoyl glycerol (DiC8), induce translocation of PKC isozymes from cytoplasm to plasma membrane or into nucleus. The activated PKC negatively modulates growth of human breast cancer cells. Antiproliferative effect and translocation of PKC were investigated in MCF-7 cells. The translocation of activated PKC isozymes by PMA, bryostatin and DiC8 was occurred at the various different regions in MCF-7 cell. PKC
could be translocated to the nucleus or the nuclear mem-brane, and PKC
to cell membrane by PMA while DiC8 and bryostatin induced the translocation of PKC
to the nucleus or plasma membrane, respectively. In the antiproliferative effect of PKC activators, PMA (
/ values of 1.2
0.3nM) and DiC8 (
/ values of 5.0
M) inhibited the cell growth. Bryostatin also inhibited the cell growth but to a much less degree than one obser-ved with PMA : 16% growth reduction by 100nM bryostatin. However, PMA treated with bryostatin induced gro-wth inhibition, but PMA with DiC8 at 10
M was not effective. These results suggest that each PKC isozyme is tran-slocated to various specific sites, and that especially, PKC
isozyme plays an important role in control of antiprolife-raive function of cell growth.
Antimicrobial Characteristics Against Spoilage Microorganisms and Food Preservative Effect of Cinnamon (Cinnamomum cassia Blume) Bark Extract
Journal of Life Science, volume 8, issue 6, 1998, Pages 648~653
The development of natural food preservatives instead of chemical synthetic food preservatives is world wide inte-rest. Authors already investigated that cinnamon bark extract revealed antimicrobial activity against general spoilage microorganisms of food especially its acitivity was stronger against molds than against bacteria. In this paper, authors examined the mirobial flora from the spoiled fish meat paste products and also checked the possibility of cinnamon bark extract food preservative for prolong the shelf life of the fish paste product and breads. The predominat bacteria was Bacillus sp. as about 98% of the total microorganisms isolated from unpacked or packed spoiled fish meat paste products. While molds and yeast are not detected from the vacuum packed products. The MIC(minimum inhibitory concentration) of cinnamon bark extract against the isolated spoilage bacteria and molds was 160~640
, respectively. When the diluted cinnamon bark extract (the extract : ethanol=1 : 3) was sprayed on the surface of fried fish meat paste product, molds growth was delayed by 2 days at room temperature. The shelf lifes of sandwich and glutinousrice bread which surface sprayed with the diluted extract(1 : 1) was extended by 5 and 7 days, respectively.
Identification of a Potential Tyrosine Phosphorylation Site on the NR2B Subunit of the N-methyl-D-aspartate Receptor
Il Soo Moon ; Yong Wook Jung ; Bok Hyun Ko ;
Journal of Life Science, volume 8, issue 6, 1998, Pages 654~659
The 2B subunit of N-methyl-D-aspartate (NMDA) receptors (NR2B) is the major phosphotyrosine-containing pro-tein in the postsynaptic density (PSD). In order to identify the site for tyrosine phosphorylation on NR2B, a mass spectrometry was applied on tryptic and endolys-C peptides. The NR2B subunit was isolated from N-octyl glucoside (NOG)-insoluble PSD fraction through SDS-PAGE and electroelution. The eluted protein was confirmed to be NR2B and phosphorylated on tyrosine by its cognate antibody and phosphotyrosine-specific antibody. By matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of the peptides generated by digesting the eluted NR2B with trysin or endolys-C, a potential site for tyrosine phosphorylation could be identified as Tyr-1304.
Effect of Paraquat on Enteroendocrine Cells in the Gastric and Small Intestinal Mucosa of Developing Rat : Immunohistochemical Study
Journal of Life Science, volume 8, issue 6, 1998, Pages 660~666
The effect of paraquat(1,1´-dimethyl-4,4'-bipyridinium) on the gastrin-, somatostatin-, glucagon-, PP-, CCK-8- and serotonin-immunoreactive cells in the stomach and small intestine of developing rat was examined by peroxidase-an-tiperoxidase method. Oral administration of this herbicide(9mg/Kg per day in 0.2ml of D.W) on days 7 to 14 of gestation revealed some difference, such as first appearance and distribution pattern of immunoreactive cells between control and paraquat-treated group. These results suggest that indirectly treated fetuses display a general developmental retardation on enteroendocrine cells differentiation as well as on gastrointestinal maturation
Isolation of Influenza Virus from Patients with Respiratory Disease in Pusan in 1997
Journal of Life Science, volume 8, issue 6, 1998, Pages 667~672
This study was done to detect the causative agent of patient with respiratory disease in Pusan, 1997. Male and female patients with respiratory disease in Pusan, 1997, were 31.9% and 68.1 ％, respectively. In the aspect of out-break by month, patients with respiratory disease were mostly concentrated at February, March, April, October, November and December. Fifteen strains of influenza virus were isolated from 1,268 swabbed samples of throat, and thirteen strains and 2 strains among 15 isolates were classified with influenza A and B virus, respectively. One of 13 influenza A virus was confirmed as A/Johannesburg/33/94- like strain, and the other isolates of influenza A virus were confirmed as A/sydney/05/97-like strains. Two isolates of influenza B virus were confirmed as B/Bei-jing/08/93-like strains.
Studies on the Adenosine Deaminase Gene from Nocardioides sp. J-326TK
Journal of Life Science, volume 8, issue 6, 1998, Pages 673~680
Adenosine deaminase gene from Nocardioides sp. J-326TK was cloned by polymerase chain reaction using primers (PI, PII and PIII) constructed from the highly conserved amino acid sequences among Escherichia coli, mouse and human. A PCR product of about 800bp, as expected from the sequence of E. coli adenosine deaminase gene, was obtained from Nocardioides sp. J-326TK chromosomal DNA double-digested with EcoRI and Pst I. DNA sequencing of the PCR product after cloning into pT7Blue T-vector shows 99.5% and 98.9% homologies in nucleotide and amino acid sequences, respectively, with the E. coli adenosine deaminase whereas 59.5% and 46.8% homologies with the human adenosine deaminase, indicating the evolutionarily relationship of these organisms.
A Study on the X-ray Diffraction of Rabbit Glycerin Muscle by Spin Labeled on SH
Journal of Life Science, volume 8, issue 6, 1998, Pages 681~686
IASL(iodo acetamide) and MSL(maleimide) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. Equatorial reflection(10,11) change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of 143
could offer infor-mation of the mass projection of population of myosin heads along the filament axis. The slope of intensity profile of the mass projection of 143
and reflection of IASL is appeared and that of MSL is appeared sharply. The dec-rease of 215
reflection intensity the periodical characteristic of 143
reflection by spin label. The raise of MSL actin reflection at 51
in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we could conclude that LASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.
Genetic Diversity and Population Structure of Chimaphila japonica in Southern Part of Korea
Joo-Soo Choi ; Man-Kyu Huh ;
Journal of Life Science, volume 8, issue 6, 1998, Pages 687~694
Enzyme electrophoresis was used to estimate genetic diversity and population structure of Chimaphila japonica Miq. in Korea. The percent of polymorphic loci within the enzymes was 48.7%. Genetic diversity at the species level and at the population level was high (Hes=0.278 ; Hep=0.222, respectively), whereas the extent of the population divergence was relatively low (
, a measure of the deviation from random mating within the 7 populations, was 0.355. An indirect estimate of the number of migrants per generation (Nm=2.61) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Factors contributing to the high levels of genetic dive-rsity found in the entire species of C. japonica include wide distribution, long-lived perennials, ability to regenerate due to rhizomatous spread, outcrossing induced by animal vectors, and occasional pollen dispersal by wind.
Comparison of Characteristics of Hepatic Microsomal Cytochrome P45O-dependent Monooxygenases from Snake and Rat
Ja Young Moon ; Dong Wook Lee ; Ki Hyun Park ;
Journal of Life Science, volume 8, issue 6, 1998, Pages 695~701
This study was carried out to investigate levels of the components of microsomal mixed function oxidase (MFO) system and activities of the hepatic microsomal cytochrome P45O (P45O)-dependent monooxygenases of grass snake (Natrix tigrina Lateralis) and to compare with those of rat. The levels of P45O and cytochrome b
) of snake were much lower than those in rat. NADPH-cytochrome c reductase activity in the snake was also only 40% of that in the rat. Activities of 7-ethoxycoumarin 0-deethylase (ECOD) and benzphetamine N-demethylase (BPDM) of snake hepatic microsomes, when compared with those of rat, were markedly low. But, aryl hydrocarbon hydroxylase (AHH) and testosterone hydroxylase (TSH) activities were nearly the same or higher than those of the rat. Of the P45O-dependent TSHs measured, 7
-hydroxylase activity was the highest in snake, whereas, 6
-hydroxylase activity was the highest in rat. However, stereoselectivity of the enzyme from the snake to C2 and C6 positions of testoste-rone was the same as rat. The result of radioimmunoassay (RIA) for the identification of five P45O isozymes with MAbs shows that relatively high content of ethanol-inducible P45O isozyme, CYP2El, exists in the rat, whereas MC-inducible P45O isozyme, CYP2A1/1A2, does in the snake. From the analyses of SDS-PAGE and RIA of partially pu-rified P45O, we suggest the possibility of the presence of a certain P45O isozyme(s) in hepatic microsomes of snake different from those of rat.
Small Metal Ion Effect on The Harvest of Perilla Leaves in Aquiculture
Journal of Life Science, volume 8, issue 6, 1998, Pages 702~710
The perillas were cultivated to investigate for the small metal ion effect on the harvest of perilla loaves in aquicul-ture system in the constant flow rate. The perillas were und-ergrown at the condition of low concentration of KNO
as below 270ppm and 152ppm respectively. The high concentration of the metal and non-metal ca-tions of
are influenced to the growth of plant length of perillas at the earlier time. The low concentration of the metal cations of
are influenced to lower growth of perillas. The concentration of the cations of
and the anions of
are affected the growth of leaf length and width of leaf of perillas. The spectoscopic analytical results showed that the perillas were growing rapidly in the period of 6 days from June 7 to June 12 by high amount of
ion with accumulation inside perillas. The crude protein, the crude fat and the hydrocarbon are accumulated in the leaves of perillas by binding the inorganic with amino acids to provide the nutritions needed for growth of perillas.
Antimicrobial Susceptibility of Several Bacterial Species Isolated from Human Blood Culture
Journal of Life Science, volume 8, issue 6, 1998, Pages 711~722
This study was undertaken to determine the antimicrobial susceptibilities of 716 bacterial strains among 1,104 microbes which were isolated in blood culture from 13,595 in-patients and out-patients in K hospital during the pe-riod of 1993 to 1996. The results obtained were as follows : The isolation rate of microbes from the blood culture for 3 years was 8.1% (1,104/13,595). Among 716 bacte-rial strains, Escherichia coli and Staphylococcus epidermidis were 29.8% (213) and 19.5% (140) respectively. The bacterial isolation frequency according to ages was high at fifties, sixties and two or less years old groups, especially the isolation rate of S. epidermidis was 47% at two or less years old group and that of Salmonella typhi was 36% at thirties years old group. The seasonal isolation rate of the bacterial species except E. coli was high during May to September. Gram positive cocci were resistant to penicillin-G except Enterococcus faecalis, but susceptible to vanco-mycin and teicoplanin. Gram negative bacilli were susceptible to amikacin, ciprofloxacin, and imipenem, but resistant to ampicillin except S. typhi. These results indicate that bacterial strains isolated from bacteremia patients were resis-tant to penicillins, but susceptible to vancomycin, teicoplanin, amikacin, ciprofloxacin and imipenem.
-Lactam Antibiotics Resistant Bacterium
Journal of Life Science, volume 8, issue 6, 1998, Pages 723~728
Bacillus subtilis J105, high resistant bacteria against
-lactam antibiotics, become higher resistant through induction of
--lactamase in the presence of
--lactam antibiotics. When there is no antibiotics in medium, the production of resistance-inductive
--lactamase reached its plateau 15 hours later. But when there is ampicillin (500
) in medium, the production of enzyme reached its plateau 25 hours later since cultivating bacteria, whereas it is found that enzyme 2,900 units/
about 20 times as much as compared with not-presence of antibiotics was actived. In addition, as the result of MIC comparing applying ampicillin-treated and non-treated strain MIC of ampicillin-treated strain is about 2~27 times higher. It is considered that this strain induce
--lactamase production by ampicil-lin-treatment, then increasing it resistance. It is found that this resistant strain induce
--lactamase production against cephalosporin antibiotics as well as peni-cillin. As the result of examining the time of adding antibiotics for each phase of growth, it is concluded that logarith-mic phase is the most effective. As the aboves, it is suggested that this strain is a peculia strain that its resistance is induced high by various
Health Policy for The Senile Dementia in Korea
Journal of Life Science, volume 8, issue 6, 1998, Pages 729~736
The purpose of this study is to examine the senile dementia in Korea and then provides policy alternatives for the heal-thy life of the aged. The increaase of life expectancy is the hope for all, accordingly unhealthy life will be misfortune for them including their family. The physiological dysfunction of human body causes diverse problems. Among them, the most serious one is the malfunction of brain to include both Alzheimer's disease and senile dementia. When considering the poor analysis for senile dementia and the non-systematic aspect of national health policy for the aged, this paper has concluded that more planned programs should be developed for the aged in viewpoint of national criteria.
Effect of Oral Administration of Fibrinolytic Enzyme from a Fermented Anchovy, Myulchi Jeot-Gal
Journal of Life Science, volume 8, issue 6, 1998, Pages 737~740
Effect of oral administration with fibrinolytic enzyme isolated from fermented anchovy(the traditional fermented food in Korea called Myulchi Jeot-gal) and its functionally active enzyme to rat, activation of plasma fibrinolysis was observed. The euglobulin fibrinolytic activities and the plasma levels of H-D-Val-Leu-Lys-pNA(S-2251) amidolysis reached a maximum at 3 hours after the administration to rat. And euglobulin Iysis time(ELT) value after oral admi-nistration showed its activity 2∼3 hours later. From the above result, it was confirmed the enzyme activity in blood by oral administration fibrinolytic enzyme through animal experiment.