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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 9, Issue 6 - Dec 1999
Volume 9, Issue 5 - Oct 1999
Volume 9, Issue 4 - Aug 1999
Volume 9, Issue 3 - Jun 1999
Volume 9, Issue 2 - Apr 1999
Volume 9, Issue 1 - Feb 1999
Selecting the target year
Isolation of Antagonistic Bacteria to Phytophthora capsici for Biological Control of Phytophthora blight of Red Pepper
Journal of Life Science, volume 9, issue 1, 1999, Pages 1~7
To isolate of antagonistic bacteria to Phytophthora capsici, which cause Phytophthora blight in red pepper, 237 isolates of Pseudomonas spp. and 260 isolates of Bacillus spp. were screened in selective media from rhizosphere soils of red pepper at Kyongsan, Kyongju, Yongchon and Euisung in Kyongbuk. Among total 497 isolates, 8 isolates of Pseudomonas spp and 4 isolates of Bacillus spp. inhibited the mycelial growth of Phytophthora capsici above 50
. These antagonistic bacteria showed more inhibitory effect on TSA (tryptic soy agar) than V-8 juice agar. Four isolates, P0704, P1201, B1101 and B1901, showing the most prominent antagonistic activity were selected and identified as P. cepacia (P0704, P1201), B. polymyxa (B1101) and B. subtilis (B1901), respectively. Cell free filtrates of these isolates were shown to inhibit zoosporangia germination and mycelial growth of p. capsici indicating that these isolates turned out to be bacteria producing antifungal substances. As a result of antagonistic test to Phytophthora blight in green house p. cepacia (P0704) showed the highest antagonistic effect with 46.7
and the rest of them were in the range of 13.4
Antifungal Activity and Cultural Characteristics of the Streptomyces sp. A252
Journal of Life Science, volume 9, issue 1, 1999, Pages 8~14
The growth rate of the A252 strain was increased in tryptic soy broth (TSB) and malt extract-yeast extract medium (ISP-2), but the antifungal activity of culture filtrate was efficient in the media of TSB and nutrient broth. The mycelial growth and the antifungal activity of culture filtrate in TSB medium were optimized at
and pH 6.5. The growth in 2
TSB concentration was more effective than 1
, but there was no difference of the antifungal activity by the TSB concentrations. The mycelial growth of A252 strain reached to maximum at 72 hr after inoculation, whereas the antifungal activity of culture filtrate was shown to have the highest level at idiophase (60 hr) after inoculation and was decreased a little after 96 hr incubation. The antifungal activity was stable in the pH range of 4 to 11 and evenly at
. The A252 strain was characterized as Streptomyces species by the physiological properties and examination of sporophore me morphology.
Limits of Direct PCR Amplification from Seaweeds Using Arbitrary and ITS Primers
Journal of Life Science, volume 9, issue 1, 1999, Pages 15~21
The random amplified ploymorphic DNAs (RAPD) assay is a simple and useful tool in identification of appropriate genetic markers, that requires no knowledge of target DNA sequence. RAPD products were generated directly from seaweed tissues, without prior nucleic acid extraction, of Porphyra yezoensis, Ulva pertusa and Undaria pinnatifida. The nuclear rDNA internal transcribed spacer (ITS) fragment however was not amplified directly from the seaweed tissues. Using DNA extracted by the LiCl method, both the ITS and RAPD's have been amplified by the polymerase chain reaction. RAPD of P yezoensis, thallus (n) and conchocelis (2n) produced lots of different polymorphic bands (36-50
) depending on the arbitrary primers used. Difference was also observed between direct tissues amplification and DNA extracts amplification (53-57
). Thus it is important to use the same ploidy of tissue for DNA extraction and as a RAPD template.
Isolation and identification of Exo-Inulinase Producing Bacterium and Optimization of the Enzyme Production
Journal of Life Science, volume 9, issue 1, 1999, Pages 22~28
A bacterium producing exo-inulinase was isolated from soil and identified Pseudomonas sp. and named as Pseudomonas sp. NO5. The optimal culture conditions for the efficient production of exo-inulinase from Pseudomonas sp. NO5 were obtained by cultivating with the medium 1
yeast extract, 0.5
in initial pH 7.0 for 20 hours. The enzyme was induced maximally in the presence of sucrose or inulin at early stationary phase about 20 hour after cultivation.
Changes of RNA and Protein During Callus Induction and Plant Regeneration from Perilla frutescens
Journal of Life Science, volume 9, issue 1, 1999, Pages 29~34
Cotyledon and hypocotyl explants of perilla were cultured on MS medium containing a combined concentration of BA(0.5, 1.0 and 1.5mg/
) and NAA(0.1, 0.5 and 2.0mg/
) in order to regenerate the explant and induce the callus. The best regeneration of the explant and induction of the callus were observed in a combined concenteration of 0.5mg/
of BA and 0.5mg/
NAA both in cotyledon and hypocotyl explants. In cotyledon explants, rooting was achieved upon transferring shoots to MS medium containing 0.5mg/
of BA and 0.1mg/
of NAA. We also investigated the change of protein and RNA content on developmental stage of callus and plant regeneration of perilla. Protein content was increased but RNA content was decreased as the culture period increases. The banding pattern of polypeptide revealed that both 30KD and 45KD polypeptides were obvious in cotyledon obtained from pre-culture explants, but only 30KD polypeptide was further getting obvious as the culture period increases.
Leaf Protein Affect the Concentration on Serum Cholesterol and Free Amino Acids in Rats
Journal of Life Science, volume 9, issue 1, 1999, Pages 35~43
Leaf protein (LP) was prepared by centrifugation the green juice from Italian ryegrass (Lolium multiflorum Lam.) at acid precipitation. LP was compared with casein and soybean protein isolate for their concentration of serum lipids and free amino acids in Wistar male rats for 2 weeks. Each protein source was incoporated into cholesterol free, low-fat diet to provide a protein level of 20%. A comparison of serum amino acids in leaf protein-fed vs. casein-fed rats showed that, glycine was the only amino acid having a higher concentration whereas concentration of other amino acids were lower in the leaf protein-fed rats compared with the casein-fed rats. Further alanine was significantly lower in the leaf protein-fed rats compared with the casein-fed rats, and the protein-induced differences in glycine and alanine concentration of unfed rats were reproducible. Concentration of several amino acids, rarticularly valine, leucine and tyrosine, also changed when serum cholesterol concentrations varied, but these effects could not be explained by our experiments. The results suggest that a change in serum concentration of glycine and alanine of unfed rats may be related to the change in serum cholesterol concentration. These data also suggest appear to function in a similar way to soybean protein in their hypocholesterolemic action.
Preparation of Seasoned and Semi-Dried Right-Eyed Flounder and Quality of Its Product during Storage
Journal of Life Science, volume 9, issue 1, 1999, Pages 44~49
A study was conducted to investigate the possibility of processing seasoned and semi-dried right-eyed flounder (Pseudopleuronects herzensteini) and to examine its quality during storage at
by analyzing the factors such as moisture content, pH, volatile basic nitrogen (VBN), viable cell count and peroxide value (POV). For processing of the seasoned and semi-dried product, optimum conditions of drying temperature and drying time were
and 4 hrs, respectively, under 50
of relative humidity and 2 m/sec of air blast speed. The shelf-lives of the products by vacuum-packaging method in nylon/polyethylene/linear low density polyethylene (0.015/0.045/0.040 mm) laminated film bag were 35 and 16 days during storage at
Analysis of the Role of STAT Binding Site in the Drosophila raf Promoter Region Using Transgenic Flies
Park, Hyun Sook ; Kim, Young Shin ; Kwon, Eun Jeong ; Yoo, Mi Ae ;
Journal of Life Science, volume 9, issue 1, 1999, Pages 50~57
STATs activated by various cytokine and growth factors trigger a quick response in the nucleus and induce changes in gene expression. We have found the sequences homologous to STAT binding site in the 5'-flanking region of the D-raf gene. In this study, we examined role of the STAT binding site in D-raf gene promoter activity in vivo by using transgenic flies. The reporter plasmid pDraf-STATmut-lacZ was constructed by fusing D-raf promoter fragment having the base-substituted STAT binding site with the lacZ gene in a P-element vector. Transgenic flies bearing the Draf-STATmut-lacZ fusion genes were established by P-element mediated transformation. The expression of lacZ in transgenic flies bearing Draf-STATmut-lacZ fusion genes carrying base substitution in STAT site throughout various developmental stages was extensively reduced in comparison with that in transgenic flies bearing wild type Draf-lacZ fusion gene. These results show that the STAT binding site plays an important role in regulation of the D-raf gene.
Antitumor Activities of Lipophilic Nucleoside 5′-monophosphate Analogues as Prodrugs
Lee, Bong-Hun ; Park, Jang-Su ; Kang, Shin-Won ;
Journal of Life Science, volume 9, issue 1, 1999, Pages 58~62
Several nucleoside 5'-monophosphate analogues and lipophilic nucleoside 5'-(3-pyridinylcarbonyl)monophosphate analogues were synthesized. Antitumor activities of the synthesized nucleoside malogues against P338 mouse leukemia, FM3A murine mammary carcinoma, and U937 human histiocytic lymphoma cells were determined by MTT assay. Antitumor activities of the lipophilic uridine 5'-(3-pyridinylcarbonyl) monophosphate(7) and 2',3'-didehydro-3'-deoxy-thymidine-5'-(3-pyridinylcarbonyl) monophosphate(8) were stronger than those of uridine 5'-monophosphate(1) and 2',3'-didehydro-3'-deoxythymidine-5'-monophosphate(4). This preliminary experimental result suggests that nucleoside 5'-(3-pyridinylcar-bonyl)monophosphate analogues may be new prodrugs to overcome the clinical limit.
Syntheses and Biological Activities of Uridine Nucleoside Derivatives
Bong-Hun Lee ; Jang-Su Park ; Shin-Won Kang ;
Journal of Life Science, volume 9, issue 1, 1999, Pages 63~68
Many nucleoside compounds such as 5-halogen substituted uridine, 5'-amino-5'-deoxyuridine conjugates of amino acid, peptide, and penicillin G, 5'-monophosphate uridine derivatives and 5'-monophosphate uridine-fatty acid derivatives were chemically synthesized and their antifungal, antibacterial, and antitumor activities were tested. 5-Bromo-2',3'-O-isopropylideneuridine(6) inhibited the growth of Trichophyton rubrum at
g/ml of MIC. 5'-Amino-5'-deoxyuridine-penicillin G(19), 5'-amino-5'-deoxyuridine-cyclo(Phe-Asp)(20), and 5-iodo-5'-amino-5'-deoxyuridine- penicillin G(22) had antibarterial activity(MIC was
g/ml against S. aureus) and the latter two nucleoside compounds were the most antitumor derivatives(their
against L5178Y murine lymphoma cell was
Genetic Relationship within the melanogaster Complex of the Genus Drosophila
Journal of Life Science, volume 9, issue 1, 1999, Pages 69~75
Four species belonging to the Drosophila melanogaster complex were examined genetically and morphologically to analyze interspecific relationships. Insemination rates ranged from 96% to 99% within species crosses, but interspecific crosses among the four species exhibited a great variations in the frequency of successful matings. D. melanogaster females mated relatively well with males of other species and D. sechellia males were more successful in mating with females of other species. In the crosses among D. simulans, D. mauritiana and D. sechellia, hybrid flies were fertile in females, but sterile in males regardless of reciprocal matings. The phenogenetically relationship between this complex and their hybrids were investigated by the comparison of sex comb tooth number and genital arch of male. They were controlled by polygenic factors on the chromosome of both parents. The effects of temperature on viability of hybrids between D. melanogaster females and D. simulans males were investigated for detection of genes concerning the speciation. The temperature sensitivity of the hybrid was mainly controlled by genes located on the X chromosome of D. simulans males.
Overexpression of ER Resident Molecular Chaperones and Characterization of Their Interaction with Thyroglobulin in FRTL5 cells.
Seong, Yeon-Mun ; Shong, MinHo ; Kwon, O-Yu ;
Journal of Life Science, volume 9, issue 1, 1999, Pages 76~83
Mammalial expression vectors containing GRP94, BiP, ERp72, and PDI, were introduced into FRTL5 cells. Transfected cells were selected by neomycin resistance for exogenously overexpressed proteins in the ER. The use of a reducible cross-linker, DSP, markedly improved the ability to detect noncovalent interactions of PDI, BiP and GRP94 with newly-synthesized thyroglobulin. Under normal conditions, GRP94 was found to associate transiently with early Tg folding intermediates, displaying interaction kinetics similar to those reported for another ER chaperones of BiP.
Microbial Degradation of Hydrocarbons in the Waste Oil
Journal of Life Science, volume 9, issue 1, 1999, Pages 84~91
Sediment samples from the waste-oil spilled sites were screened for microorganisms able to degrade the components of crude oil, and 3 strains that could degrade were obtained. The isolated 3 strains (Xl, X2 and X3) metabolized naphthalene and 2-methyl naphthalene about 80
as well as hexane and hexadecane about 60~70
as a sole carbon source in 7 days. The degradation of the waste oil was about 60
. The addition of synthetic surfactant, Triton-X 100 or Tween 20 slightly inhibited the growth of the populations. Xl and X2 were gram negative and X3 was gram positive. Xl and X3 showed ampicillin resistancy. Xl strain having 30kb plasmid has been selected for genetic study. The plasmid was isolated and transformed into E. coli. showing the possibility of the genetically engineered degrader.
The Effect of Saengmaeksan on the Recovery of Exercise Induced Fatigue
Journal of Life Science, volume 9, issue 1, 1999, Pages 92~98
This study was aimed to evaluate the effect of saengmaeksan on the recovery of exercise induced fatigue. In this experiment, white rats were divided into 2 groups; control group and Saengmaeksan treated group. The results were as follows: 1. Oxygen consumption was significantly decreased in Saengmaeksan treated group than in control. 2. Serum lactate levels were significantly decreased in Saengmaeksan treated group than in control. 3. Serum LDH activities were significantly decreased in Saengmaeksan treated group than in control. 4. Serum triglyceride levels were significantly increased in Saengmaeksan treated group than in control. 5. Serum glucose levels were significantly increased in Saengmaeksan treated group than in control. 6. Serum FFA levels were significantly decreased in Saengmaeksan treated group than in control. According to the results, saengmaeksan showed remakable effects in the recovery of exercise induced fatigue.
Discovery of Antioxidant on Human Low Density Lipoprotein (LDL) by Bacillus sp. RH-5 Isolated from Marine Origin
Journal of Life Science, volume 9, issue 1, 1999, Pages 99~105
The aims of this studies were carried out to investigate the antioxidant activity on low density lipoprotein(LDL) using substances extracted from Bacillus sp. RH-5. The antioxidative substances produced extracellular in the culture broth by Bacillius sp. RH-S was obtained by elution of chloroform : methanol from silicagel column (80cm x100cm) chromatography. Band 4 eluted from fraction 3 by TLC was appeared at highest level of antioxidative activity using thiocyanate methed. Band 4 at a concentration of 100 or 200
inhibited oxidation of LDL induced by the mouse transformed macrophage. According to IR. NMR or GC/MASS, the antioxidant substance was identified as 5-hydroxyindole.
Cytotoxic Effect and Fatty Acid Composition of Lipopolysaccharide from Vibrio vulnificus
Lee, Bong-Hun ; Park, Jang-Su ; Shin, Won-Kang ;
Journal of Life Science, volume 9, issue 1, 1999, Pages 106~110
Lipopolysaccharide(LPS) from Vibrio vulnificus was purified, the fatty acid composition was analyzed, and Limulus gelation activity and lethal toxic activity were tested in order to investigate the cause of cytotoxicity by V. vutnificus. These results were compared to those of Escherichia coli LPS and Salmonella typhimurium LPS. LPS from V. vulnificus had a different fatty acid composition from those of E coli and S. typhimurium. The major fatty arid from each LPS was lauric acid for E. coli, rapric acid for S. typhimurium, and myristic acid for V. vulnificus. The Limulus gelation activities of three LPSs were the same(0.1ng/ml) and the lethal toxicity in BALB/c mouse of V vulnificus LPS was similar to those of E. coli LPS and S. typhimurium LPS. Such factor as exotoxin need to be considered to be the cause of cytotoxicity by V. vulnificus LPS.
Characterization of Bacteriocin Production by Lactococcus sp. J-105 Isolated from Kimchi
Journal of Life Science, volume 9, issue 1, 1999, Pages 111~120
A bacteriocin-producing strain, J-105, was isolated from Kimchi and identified as Lactococcus sp. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For maximum yield of bacteriocin from Lactotoccus sp. J-105, the cell should be harvested at the early stationary phase and temperature, pH and NaCl concentration should be
, pH 8.0 and without the addition of NaCl, respectively. Maltose should be used as a carbon source and organic nitrogen such as polypeptone should be used as a nitrogen source for the best yield. The bacteriocin from isolate was inhibitory against Acetobacter aceti, Bacillus subtilis and several strains of lactic acid bacteria. The bacteriocin of J-105 was sensitive to pepsin, but stable for heat treatment. It was stable even at autoclaving temperature for 15 min.