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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Life Science
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Journal DOI :
Korean Society of Life Science
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Volume & Issues
Volume 9, Issue 6 - Dec 1999
Volume 9, Issue 5 - Oct 1999
Volume 9, Issue 4 - Aug 1999
Volume 9, Issue 3 - Jun 1999
Volume 9, Issue 2 - Apr 1999
Volume 9, Issue 1 - Feb 1999
Selecting the target year
Molecular Cloning and Characterization of the Gene Encoding Chitinase from Bombyx mandarina
Journal of Life Science, volume 9, issue 4, 1999, Pages 341~347
Insects use chitinolytic enzyme to digest chitin in the exoskelton during the molting process. We have isolated and sequenced a chitinase-encoding cDNA from the silkworm, Bombyx mandarina, compared its sequenced with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 2,675 nucleotides long with an open reading frame of 1,695 uncletides that encodes a protein of 565 amino acids with a molecuar weight of 63.4 kDa. The 3' -untranslated region of 889 nucleotides is AT-rich and contains two putative polyadenylation signals. The N-terminal sequence of the encoded protein contains numerous hydrophobic residues characteristic of a leader peptide. The amino acid alignment revealed that the endo-
-N-acetylglucosaminidase had 83% and 97% homology with M. sexta and B. mori, respectively. The deduced amino acid had two highly conserved region at the amino acid residues 97-111 and 139-148 that were related to the existing chitinase.
Studies on the Pseudomonas aeruginosa Isolated from Infected Patient
Journal of Life Science, volume 9, issue 4, 1999, Pages 348~357
Seventy-two strains of Pseudomonas aeruginosa isolated from the patients were tested for pigment production, exoenzyme production and antimicrobial susceptibility. In the pigment production test, 23.6% of total 72 strains produced both pyocyanine and pyoverdin. Pyoverdin and pyomelanin producing strains were in 9.7%, and 5.5% produced pyoverdin and pyorubin. Strains producing of all of exoenzyme, protease, elastase and lecithinase were in 5.6%. The most common type of exoenzyme production was both protease and elastase producing. Protease producing strain were 23.6%, Among the 72 strains, 50% produced protease. As the result of antimicrobial susceptibility in the isolated 20 strain, most strain were resistant to sulfamethoxazole(90%), but sensitive to other tested antibiotics more than 60%. The MIC50 and MIC90 level of tested antibiotics to 70 strains were 128
for KM, 8,256
for GM, 8, 128
for CPZ, and 8, 64
for PIPC respectively
Comparison between Dot Blot Hybridization and Southern Blot Hybridization in Detecting Methicillin Resistant Staphylococcus aureus
Journal of Life Science, volume 9, issue 4, 1999, Pages 358~367
Thirty strains of methicillin resistant Staphylococcus aureus were obtained from the clinical isolates. In order to investigate the pursuit of the pathogens of nosocomial infection, these strains were studied for antibiotic sensitivity as well as its resistant pattern. Among the methods of hybridization which directly confirm the specific antibiotic resistant genes by means of the recently developed specific probe DNA, dot blot hybridization and southern blot hybridization were performed and these two methods were compared in their sensitivity and specificity. Strains that is sensitive to cephalothin to the subject of methicillin resistant Staphylococcus aureus were in 43%. Those that are sensitive to cefoperazone and cefuroxime were 26% and 23%, respectively. In case of MIC, MIC50 of cefoperazone was 8
, and MIC90 was 128
to be the lowest. As the results of plasmid DNA electrophoresis, most of methicillin resistant Staphylococcus aureus strains had more than 4 plasmids. These plasmids digested by BamHI, methicillin resistant Staphylococcus aureus is distributed as 10 fragments with the size of 65 kb to 1.5 kb. Dot blot hybridization were performed to examine the existence of mecA gene to show the detection rate of 50%. Southern blot hybridization were done to see if DNA bands which amplify the activity of digoxigenium-labeled probe by PCR were actually PCR products of mecA gene and it showed the detection rate of 53%. It can be concluded that the southern blot hybridization seemed to be better in sensitivity and specificity when it is compared with the results of dot blot hybridization.
Ecophysiological Studied on the Matter Production of Soybean to the Environmental Stress
Journal of Life Science, volume 9, issue 4, 1999, Pages 368~374
Three soybean cultivars, Hwangkeum, Tanyeob and Enrei were planted in the same pot under glasshouse conditions to investigate the influence of the different soil water content such as pF 1.4(wet), 2.1(control) and 3.6(dry) on the transpiration rate, dry matter production and water requirement. The transpiration rate remained the high constant rates under the wet soil condition and the control than the dry condition, and showed a linear correlation between transpiration rate and solar radiation under the all condition of soil water. The transpiration rate highly increased in the morning, but dramatically decreased in the other time in a day. The dry matter production was higher under the conditions of wet soil and the control than that under the dry condition. Also, the dry matter production Tanyeob was higher than other cultivars under all soil water content. The water requirement was higher for Enrei and lower Tanyeob than the control.
Effects of Bombyx mori Larvae Extracts on Carbon Tetrachloride-Induced Hepatotoxicity in Mice
Journal of Life Science, volume 9, issue 4, 1999, Pages 375~381
To evaluate the protective effects of extracts of silkworm on carbon tetrachloride-induced hepatotoxicity, serum glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase and lactic dehydrogenase activities, malondialdehydes values and glutathione-S-transferase activity were measured in ICR mice. Extracts of silkworm was administered orally at 30min after the administration of CCl4 Mice were sacrificed at 24h after the administration of extracts of silkworm. The activities of serum aminotransferase and the hepatic content of lipid peroxide after carbon tetrachloride treatment were markedly increased than normal control but those levels were decreased by the treatment of butanol soluble fraction of silkworm methanol extract. Glutathione S-transferase activity was decreased by carbontetrachloride than control, but also inhibited by the treatment of butanol soluble fraction of silkworm methanol extract.
Effects of Citrus Flavonoid, Hesperidin and Naringin on Lipid Metabolism in HepG2 Cells
Journal of Life Science, volume 9, issue 4, 1999, Pages 382~388
The effects of citrus flavonoids, hesperidin and naringin, on the lipid metabolism were investigated in cultured human hepatocyte HePG2 cells. HepG2 cells were cultured for 6 h and 24 h to the control medium or the media containing hespridin and narigin, which concentrations were 0.5 and 5.0 mg/
. There were no significant effects on cell proliferation and cellular protein content, except for increased in these parameters by adding both citrus flavonoids (0.5 mg/
). The cellular content of triacylglycerol after 6 h incubation with 0.5 mg/
hesperidin and naringin was markedly increased, and after 24 h incubation that was decreased in both citrus flavonoids supplementation. The supplementation of 5.0 mg/
hesperidin caused a marked decrease in the cellular cholesterol content following 6 h incubation, and that was also reduced markdly, in a dose-dependent manner, during incubation for 24 h. However, there was no significant difference in the cellular cholesterol content in medium supplemented with naringin. The effect of hesperidin and naringin on acyl-CoA: cholesterol acyltransferase (ACAT) activity was studied in vivo and in vitro. The data confirmed that hesperidin inhibit ACAT activity in vivo and in vitro, whereas naringin had no such effect on ACAT activity in vivo but not in vitro. The present study suggests that hesperidin reduces the cellular triacyglycerol and cholesterol contents in human hepatocyte HepG2 cells.
Effects of Hesperetin and Naringenin on Lipid Concentration in Orotic Acid Treated Mice
Journal of Life Science, volume 9, issue 4, 1999, Pages 389~394
Male mice (ddY strain) were fed a laboratory chow diet containing 10% sucrose supplemented with orotic acid, hesperetin or naringenin at the 1% level for 14 days. the concentrations of liver triacylglycerol and cholesterol were significantly lower in the OA group than in the control group. When both flavonoids and orotic acid were administered simultaneously, the orotic acid-dependent decrease in liver triacylglycerol and cholesterol were attenuated slightly. The concentration of serum cholesterol in the orotic acid group or the control group was lower than in the orotic acid groups supplemented with hesperetin or naringenin. There were no significantly difference in body weight gain, diary food intake, and the serum concentrations of triacylglycerol and high-density-lipoprotein cholestrol. It was concluded that the inducement of fatty liver in mice failed to feeding a laboratory chow diet containing 10% sucrose supplemented with 1% orotic acid for 14 days.
The Effect of Temperature on the Colony Development of Bombus ignitus Smith
Journal of Life Science, volume 9, issue 4, 1999, Pages 395~407
The queens of Korean native bumblebee species, Bombus ignitus were collected from the field in the spring of 1997 and reared under the various temperatures as 15
5% of relative humidity and 14L : 10D, to investigate their ecological characteristics, as comparing with control(29
5% of relative humidity and red light). As a result, eggs were laid by foundation queen at 15
, but they didn't hatched out while at 2
eggs were laid by them and they hatched out. When a foundation queen laid eggs at
and they all were hatched out and normally grown. Finally they were developed to the normal colony. B. ignitus worker survived for 77days at 2
, about 69days at
, about 68days at 3
and about 63days at 29
(control). The first brood workers emerged in 25-27days from the egg which a foundation queen laid. At the various temperatures, the captive queens stand to lay eggs in about 18days at 15
, 15days at 2
, 11days at
, 11days at 3
and 4days in control. The first worker and the first drone from the egg cell of the collected queens appeared in about 27days and 72days at 2
, 26days and 88days in
, 24days and 65days at 3
, 25days and 71days in control, respectively. In colony foundation, 33% of the collected queen at 2
, 100% of the disposed queen at 3
and in control, and 67% of them at 3
and in control both produced new queens in 66days and 88days, respectively. The life span of the colony founded covered fro about 3 months at
and in control. At lower temperatures, the life span of queen is shorter; 2 months at 15
and 3 months at 2
, respectively. A colony which normally developed, varied in size with rearing temperatures; about 20heads at 2
, 482heads at
, 330heads at 3
and 452heads in control. A foundation queen monthly oviposited 1.5egg cells at 15
, 3.0egg cells at 2
, 21.7egg cells at
, 42.3egg cells at 3
and 47.0egg cells in control. As a colony developed in June and July, egg cells as well as daily average cells increased in number, as compared to those in May and August. Also, in June and July, interval(days) of egg laying is shorter than in May and August. Number of nectar pots during the colony development varied with rearing temperatures; 31pots at
, 39pots at 3
, 23pots in control. The emergence of new queen showed a distinctively different two patterns; early emerging type and late emerging type. Workers are rapidly increased from early in July to early in August at
, but at 3
and in control, emergence of workers are remarkably increased from the middle of June and last until July. No new queen emerged at 15
. New queens at 3
and in control emerged between late in June and early in July. Thus emergence of new queen was temperature dependent.
Elicitor-InduciblePhytoalexin from Cell Suspension Cultures of Pepper(Capsicum annuum L.)
Journal of Life Science, volume 9, issue 4, 1999, Pages 408~413
Extracellular capsidiol, sesquiterpenoid phytoalexin, in the medium of pepper (Capsicum annuum L.) suspension cells was not identified from control cells, but highly accumulated in the elicitor-induced cells within 6 hours after the addition of 0.05
cellulase. Capsidiol production in elicitor-induced cells was markedly suppressed by cytochrome P450 inhibitors, such as ancymidol and ketoconazole demonstrating that biosynthesis of capsidiol is catalyzed by at least on hydroxylation enzyme in the biochemical pathway. Based on protein electrophoresis, two bands, 23.0kDa and 27.5kDa, were identified as newly synthesized polypeptides in the elicitor-induced suspension cells, suggesting that pepper cells which were subjected to elicitor treatment activate specific gene(s) for capsidiol biosynthesis in cultured cells.
Purification and Characterization of Carrageenase from Pseudomonas alcaligenes JCL-43
Journal of Life Science, volume 9, issue 4, 1999, Pages 414~422
Our works performed for preparation of oligosaccharides from carrageenan, seaweed polysaccharide, and one active strain for carrageenan was isolated from sea water and identified to Pseudomonas alcaligenes. Carrageenan degrading enzyme was purified from the culture fluid of isolated strain-Pseudomonas alcaligenes JCL-43, by DEAE-Cellulose, Sephadex G-100, Q-Sepharose and CM Sepharose CL-6B column chromatography. Two enzyme-F-I, F-II- was identified this purifying process, and the molecular weight of the purified carrageenase were estimated to be 23.6kDa and 30.2kDa, respectively. The optimum pH and temperature for two carrageenase activity were 7.0 and 4
. These enzymes were stable in the pH range of 6.0~7.5 and lower than 5
, and required 1.5% NaCl for optimum activity. And these carragennase were inhibited by metal ions such as Cu2+, Zn2+, Hg2+, but increased by Ba2+ and Ca2+, and showed specificity on -carrageenan.
Preparation of Carrageenan Oligosaccharides Using Carrageenase from Pseudomonas alcaligenes JCL-43 and Its Functional Properties
Journal of Life Science, volume 9, issue 4, 1999, Pages 423~429
Carrageenan oligosaccharides prepared from -carrageenan by carrageenase from Pseudomonas alcaligenes. The oligosaccharides showed three spots on TLC and the degree of Polymerization of the C1, C2 and C3 spot were each 9.0
1.5 and 2.5
1.5, respectively. Each hydrolysates and spots-C1, C2, C3-were tested the several functionalities such as antimicrobial activity, anticavity activity and anticoagulant activity. The antimicrobial and anticavity activity of carrageenan hydrolysates and oligosaccharide fractions were very low, but the anticoagulant activity was identified in all samples.
Effects of Sea Tangle (Laminaria japonica) and Fucoidan Components on Chronic Degenerative Diseases
Journal of Life Science, volume 9, issue 4, 1999, Pages 430~438
This study was designed to investigate the effects of sea tangle (Laminaria japonica) extract and fucoidan components on chronic degenerative diseases. Sprague-Dawley(SD) male rats (210
5g) were fed experimental diets: Dasi-Ex group: dasima extract powder of 4.0% added to control diet; Fuco-I, II and III groups: fucoidan powder of 1, 2 and 3% added to Dasi-Ex group for 45 days. Triglyceride (TG) levels in serum were significantly lower (10~15%) in Fuco-I, II and III groups compared with control group. Total cholesterol levels were significantly decreased (7~10% and 15~ 35%) in brain mitochondria and microsomes of Fuco-II and III group compared with control group. LDL-cholesterol levels were remarkably decreased (20~30%) in Dasi-Ex and Fuco-I, II, III groups, but HDL-cholesterol levels were significantly increased (10~12%) in Fuco-II and III groups only compared with control group. The ratios of HDL/total cholesterol resulted in a marked increase (3 5~55%) in Dasi-Ex and Fuco-I, II, III groups, but atherogenic indices were remakably decreased (40~50%) in Dasi-Ex and Fuco-I, II, III groups compared with control group. Membrane fluidities were remarkably increased (45~70% and 38~42%, respectively) in brain mitochondria and microsomes of Fuco-II and III groups compared with control group. Administrations of fucoidan added to dasima effectively decreased TG, total and LDL-cholesterol, and atherogenic index, while also effectively increased HDL-cholesterol, HDL/total cholesterol ratio, and membrane fluidity, suggesting chronic degenerative diseases were very effectively prevented by the administration of fucoidan component.
Effects of Sea Tangle (Laminaria japonica) and Fucoidan Components on Anti-aging Action
Journal of Life Science, volume 9, issue 4, 1999, Pages 439~452
This study was designed to investigate the effects of sea tangle (Laminaria japonica) extract and fucoidan components on anti-aging action. Sprague-Dawley(SD) male rats (210
5g) were fed experimental diets Dasi-Ex group: sea tangle extract powder of 4.0% added to control diet; Fuco-I, II and III groups: funcoidan powder of 1, 2 and 3% added to Dasi-Ex group for 45 days. Hydroxyl radical (.OH) formations were significantly inhibited (10-20% and 25-30%) in serum and brain mitochondria of Dasi-Ex and Fuco-I, II and III groups compared with control group. Significant differences in .OH formations of brain mitochondria in Dasi-Ex and Fuco-I groups could not be obtained, but.OH formations of brain microsomes resulted in a significant decrease (15-20%) in Fuco-II and III groups compared with control group. Basal oxygen radical (BOR) formations were significantly decreased about 10% and 13-15% in brain mitochondria of Dasi-Ex and Fuco-I group, and Fuco-II, III groups, and also decreased about 10% and 15-20% in brain microsomes of Dasi-Ex and Fuco-I groups, and Fuco-II, III groups. LPO levels of brain mitochondria and microsomes were significantly inhibited about 10% in Dasi-Ex and Fuco-I, II groups and 15% in Fuco-III groups. Oxidized proteins (>C=O) were significantly inhibited about 10% in serum of Dasi-Ex and Fuco-I, II, III groups and brain mitochondria of Dasi-Ex group, while remarkably inhibited (30~35%) in brain mitochondria of Fuco-I, II and III groups. Nitric oxide (NO) levels were significantly inhibited (12~15%) in serum of Fuco-I, II and III groups, but there no significant difference in serum NO levels of Dasi-Ex group. Superoxide dismutase (SOD) activities were remarkably increased (30~ 60%) in serum of Fuco-I, II and III groups, but there were no significant differences in SOD activities in serum of Dasi-Ex group. Catalase (CAT) activities were significantly increased about 20% in serum of Dasi-Ex and Fuco-I, II, III groups. Mn-SOD activities in brain mitochondria were significantly increased about 17% in Dasi-Ex group, while remarkably increased 26~36% in Fuco-I, II, III groups. Cu,Zn-SOD activities in brain cytosol were dose-dependently of fucoidan increased 10%, 12% and 18%, respectively, compared with control group. These results suggest that anti-aging effects of fucoidan may play a pivotal role in attenuating a various age-related changes such as chronic degenerative disease and senile dementia.
Diagnosis of Viral Infection of Pensaeide
Journal of Life Science, volume 9, issue 4, 1999, Pages 453~459
Baculovirus(WSBV) was isolated from infected Penaeide was collected from shrimp farm at southern sea of Korea from 1993 to 1995. The Infectious virus was purified and used for diagnosis of infected shrimp. Anti-viral serum were used for immunological detection as enzyme linked immunoabsorbent assay(ELISA) and indirect fluorescent antibody technique(IFAT). In IFAT, stomach, lymphoid organ and antenae gland of infected shrimp showed fluorescent reaction. In ELISA, tissues of spontaneously infected shrimp appeared higher O.D. values than in artificial infected shrimp. Primer set was constructed from sequence of 420bp of cloned Baculovirus(WSBV) genome. Specific band for infected shrimp was detected in Polymerase chain reaction(PCR)
Study on the Reproductive Biology of the Giant Freshwater Prawn, Macrobrachium rosenbergii(De Man) III. Structure of Male Reproductive Organ and Spermatophore
Journal of Life Science, volume 9, issue 4, 1999, Pages 460~465
In the giant freshwater prawn Macrobrachium rosenbergii, the paired testes were united by the testes bridge in a H-shape. During mating male discharged two hemispermtophores from right and left genital pore and they were joined and formed a compound spermatophore in peach shape. Hemispermatophore was considered to be produced at proximal and middle vas deferens region. The compound spermatophore consisted of an eosinophilic inner matrix, a basophilic outer matrix and paired sperm mass burried in the basophilic matrix. The size of compound spermatophore was 6.4∼ 9.3 mm in length and 2.4∼5.1 mm in width regardless of carapace length. After the spermatophore was deposited on female's ventral sternum behind of 5th pereiopod, it was moved to the ventral surface of 3rd and 4th pereiopods by means of the beating of male's 1st and 2nd pleopods.
Investigation of Anti-aging Effect and Determination of Chemical Structures of Pine Needle Extract (PNE) through the Animal Experiments I. Effects of PNE on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats
Journal of Life Science, volume 9, issue 4, 1999, Pages 466~472
These studies were designed to investigate the effects of pine (Pinus densiflora Sieb et Zucc.) needle extract (PNE) on oxygen radicals and their scavenger enzymes in liver membranes of Sprague-Dawley (SD) rats as a study on investigation of anti-aging effect and determination of chemical structures of PNE through the animal experiments. Male SD rats were fed basic diets (control group) and experimental diets (0.5% and 1.0%-PNE group) for 6 weeks. There were no significant differences in hydroxyl radical (·OH) formations of liver mitochondria and microsomes in 0.5%-PNE group, while ·OH formations were significantly decreased (10% and 18%, respectively) in liver mitochondria and microsomes of 1.0%-PNE group compared with control group. Microsomal hydrogen peroxides and cytosolic superoxide radicals were remarkably decreased (20% and 20∼25%, respectively) in 0.5% and 1.0%-PNE groups compared with control group. Mn-SOD activities in mitochondria were significantly increased about 10% in 1.0%-PNE group, while Mn-SOD activities in mocrosomes were remarkably increased (16∼20%) in 0.5% and 1.0%-PNE groups compared with control group. There were no significant differences in Cu, Zn-SOD activities of liver cytosol in 0.5% and 1.0%-PNE groups, while glutathione peroxidase (GSHPx) and catalase (CAT) activities were significantly decreased (28∼30% and 15∼30%, respectively) in liver cytosols of 0.5% and 1.0%-PNE groups compared with control group. These results suggest that these PNE may play a effective role in a attenuating a oxygen radical formations and increasing a scavenger enzyme activities.
Investigation of Anti-aging Effect and Determination of Chemical Structures of Pine Needle Extract (PNE) through the Animal Experiments I. Effects of PNE on Membrane Fluidity and Oxidative Stress in Liver of SD Rats
Journal of Life Science, volume 9, issue 4, 1999, Pages 473~480
This study was designed to investigate the effects of pine (Pinus densiflora Sieb et Zucc) needle extract (PNE) on membrane fluidity and oxidative stress in liver membranes of Sprague-Dawley (SD) rats as a study on investigation of anti-aging effect and determination of chemical structures of PNE through the animal experiments. Male SD rats were fed basic diets (control group) and experimental diets (0.5% and 1.0%-PNE group) for 6 weeks. Administrations of 0.5% and 1.0%-PNE resulted in a marked decreases (15∼25% and 23∼26%, respectively) in cholesterol accumulations of liver mitochondria and microsomes compared with control group. Membrane fluidities were significantly increased (15∼25%) in liver microsomes of 0.5% and 1.0%-PNE groups compared with control group. Formations of basal and induced oxygen radicals (BOR and IOR) in liver mitochondria were significantly inhibited (11∼12% and 10∼15%, respectively) by administrations of 0.5% and 1.0%-PNE compared with control group. Lipid peroxide (LPO) levels were remarkbly decreased about 20% in liver mitochondria and microsomes of 0.5% and 1.0%-PNE groups compared with control group. Oxidized protein levels calculated with carbonyl group were significantly decreased about 15% in liver mitochondria of 1.0%-PNE group compared with control group. These results suggest that PNE may play a effective role in a attenuating a oxidative stress and increasing a membrane fluidity.
Effect of Fertilizer Level of Organic Matter on Growth and Yield in Perilla
Journal of Life Science, volume 9, issue 4, 1999, Pages 481~488
This study was conducted to survey some characteristics in growth of perilla by fertilization level organic matter. Corn stalk, acorn and chestnut were used as organic fertilizer in this study. In the height of perilla, chestnut fertilizer with 880kg/10a that R2 is 0.9996 showed the height level, and followed by acorn and corn stalk in order. Number of the harvested leaves was the highest in acorn among the three organic fertilizer by chestnut and corn stalk in order. However, number of the harvested leaves continuously was the highest in chestnut. Weight of a leaf was more effective at three kinds of organic fertilizer than control. There were no difference in the rate of dried leaf weight according to kinds of organic fertilizer. On the other hand, the rate of dried leaf weight by growth period was different. It was found that the rate of dried leaf in 30days or 110days. Diameter of perilla stem at 30cm above the ground according to kinds and fertilizer level of organic fertilizer was surveyed. However, the difference was not detected in this study.
Effect of Medium Components on the Productivity of Fibrinolytic Enzyme in Bacillus sp
Journal of Life Science, volume 9, issue 4, 1999, Pages 489~492
A bacteial strain which can produce the extracellular fibrinolytic enzyme was isolated from Jeot-Gal (anchovy) that was Korean traditional salt-fermented fish. The isolated bacterium was identified to be a strain of Bacillus sp. The optimal medium for fibrinolytic enzyme production was determined to consist of 5 g maltose, 10 g defatted soybean, 20 g sodium chloride, 1.75 g K2HPO4 per liter (pH 7.0)
Presence of Eicosapentaenoic and Docosahexaenoic Acids Content of Gull (Larus crassirostris vieillot) Eggs
Journal of Life Science, volume 9, issue 4, 1999, Pages 493~495
Eicosapentaenoic (EPA, 20:5) and docosahexaenoic (DHA, 22:6) acids of gull eggs were analyzed and the results are summarized as follows: 1. EPA and DHA were 2.25% and 4.35% in the total fatty acid of gull eggs. 2. Triglyceride fractions in the fatty acids had 0.56% in EPA and 1.59% DHA. 3. Diglyceride fractions contained 1.10% EPA and 1.97% DHA. 4. Phospholipid fractions had the most abundant amounts of 4.26% EPA and 8.13% DHA
Isolation of Bacteria from Freshwater Plant Roots
Journal of Life Science, volume 9, issue 4, 1999, Pages 525~525
The isolation and identification of bacteria from freshwater plant root system were performed. Twenty four different strains were identified by microbiological identification system. Gram negative bacteria were isolated three times more than Gram positive ones. The ratio of rod and coccus was 11:1. The similarity above 0.5 was 37.5% from the tested samples in identification. Among these isolated bacteria, three dominant strains Pseudomonas cepacia JH10, Xanthomonas maltophilia JH12, Aeromonas salmonicida JH13 were selected to investigate biochemical properties and optimal growth conditions. These strains reached maximum growth after 24-36 hr of incubation in peptone broth, and their optimal temperature was 28℃ and that of pH was between 7 and 8.
Effects of Sea Tangle (Laminaria japonica) and Fucoidan Beverages on Sociopsychological Stress
최진호 ; 김대익 ; 박수현 ; 김동우 ; 이종수 ; 유종현 ; 정유섭 ;
Journal of Life Science, volume 9, issue 4, 1999, Pages 537~537
This study was designed to investigate the effects of sea tangle (Laminaria japonica) extract (Dasi-Ex group: dry base 4.0%) and fucoidan-added (Fuco-Ⅰ, Ⅱ, Ⅲ group: fucoidan of 1.0%, 2.0%, 3.0% added to Dasi-Ex) beverages on the anti-stress action. ICR male mice (20±2g) were fed basic experimental diets and given free through water bottle filled with these beverages instead of water for 18 days including sociopsychological stress. Body weight gains were consistently lower in Dasi-Ex and Fuco-Ⅰ,Ⅱ,Ⅲ groups compared with control group, expecting in a inhibitory effect of obesity. Dasi-Ex group resulted in a significant decrease of 25% in serum corticosterone (CS) secretion, while Fuco-Ⅰ,Ⅱand Ⅲgroups resulted in a marked decreases of 45∼55% in serum CS secretion compared with control group. Noradrenaline (NA) secretions were significantly increased about 15% in Dasi-Ex group, and 20∼22% in Fuco-Ⅰ,Ⅱ,Ⅲ groups compared with control group. Significant differences in brain MHPG-SO₄levels of Dasi-Ex group could not be obtained, but Fuco-Ⅰ,Ⅱ,Ⅲ groups resulted in a marked decreases of 20∼25% in brain MHPG-SO₄levels compared with control group. Dasi-Ex group resulted in a significant increases of 20% in NA/MHPG-SO₄ratio of brain, but Fuco-Ⅰ,Ⅱ and Ⅲ groups resulted in a marked increases of 45∼60% in NA/MHPG-SO₄ratio of brain compared with control group. These results suggest that fucoidan beverage may play a effective role in a ridding of the sociopsychological stress by pivotal anti-stress effect of fucoidan.