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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 29, Issue 4 - Dec 2002
Volume 29, Issue 3 - Sep 2002
Volume 29, Issue 2 - Jun 2002
Volume 29, Issue 1 - Mar 2002
Selecting the target year
Crop Development Using Plastid Transformation
Lee, Seung-Bum ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 1~5
DOI : 10.5010/JPB.2002.29.1.001
Transgenic Tomato Plants That Overexpress Superoxide Dismutase in Fruits
Park, Eun-Jeong ; Lee, Haeng-Soon ; Kwon, Suk-Yoon ; Choi, Kwan-Sam ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 7~13
DOI : 10.5010/JPB.2002.29.1.007
Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in plants. We have developed transgenic tomato plants overexpressing a cassava SOD in fruits. Three transgenic tomato plants (one from cv. Pink forcer and two from cv. Koko) using a new vector system, ASOp :: . mSOD1/pBI101, harboring ascorbate oxidase promoter (ASOp) expressing dominantly in cucumber fruits, CuZnSOD cDNA (mSOD1) isolated from cultured cells of cassava, and nptll gene as a selectable marker were successfully developed. SOD specific activity (units/mg protein) in transgenic fruits of both cultivars was increased with maturation of the fruits. SOD specific activity of well-mature fruits in transgenic Pink forcer and Koko showed approximately 1.6 and 2.2 times higher than control fruits, respectively. The strength of SOD isoenzyme bands well reflected the SOD activity during the fruit maturation. These results suggested that SOD gene was properly introduced into tomato fruits in a fruit-dominant expression manner by ASO promoter.
Plant Regeneration from Explant Types and Cultivars of Boxthorn (Lycium chinense Mill.)
Kim, Dong-Chan ; Chung, Hae-Joon ; Min, Byung-Hoon ; Yang, Deok-Chun ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 15~18
DOI : 10.5010/JPB.2002.29.1.015
Callus and shoot formation of leaf explants from in vitro propagated shoots and field grown plants depending on the position of leaf, and four boxthron cultivars were investigated. Callus formation of explants from both in vitro shoot and field grown plants as easily achieved at the cut surfaces of explants but the callus formed from leaf of in vitro shoots was hardened as the duration of culture was proceed. Calli were effectively induced from leaves detached from the middle position of both in vitro and in vitro plants on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA, and the growth of calli were better in field grown leaves than in vitro grown leaves. Shoot formation were effectively induced from leaves detached from the upper position in vitro plants, and the middle parts of in vitro plants on MS medium containing 0.01 mg/L (NAA with 0.2 mg/ BA. There was difference on the frequency of shoot formation among four different cultivars; `Jindojaerae` was the best for shoot formation followed by `Cheonyang`, `Younghagukija` and `Cheongyangjaerae`.
In Vitro Propagation of Lilium Asiatic Hybrid `Hae Hwa` via the Formation of Shoot Clusters
Han, Bong-Hee ; Yu, Hee-Ju ; Yae, Byeoung-Woo ; Goo, Dae-Hoe ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 19~23
DOI : 10.5010/JPB.2002.29.1.019
This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid `Hae Hwa`. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.
Effect of silver nitrate(AgNO
) and polyamines on shoot organogenesis and plant regeneration of Lycopersicon esculentum cultivar, Micro-Tom
Kim, Yong-Ho ; Park, Cheol-Ho ; Park, Sang-Un ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 25~29
DOI : 10.5010/JPB.2002.29.1.025
The study was carried out to establish an efficient protocol for shoot organogenesis and plant regeneration from stem explant cultures of Lycopersicon esculentum cv. Micro-Tom. The regenerated shoots obtained from stem explant cultures on solid MS medium containing the different concentrations of BAP. The highest number of shoots (5.3) per explant and shoot growth (0.7 cm) was obtained on MS medium containing 4.0 mg/L BA. The additions of AgNO
and putrescine substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L and 50 mg/L respectively. The regenerated shoots (about 1 cm) were normal and could be easily rooted with 0.1 mg/L IBA treatment. The rooted plants were hardened and transferred to vermiculite with a 92% survival rate where they grew normally.
Adventitious Root Formation from Cotyledon in Soybean (Glycine max L.) Cultivars
Ha, Keon-Soo ; Han, Tae-Jin ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 31~36
DOI : 10.5010/JPB.2002.29.1.031
The patterns of adventitious root formation from cotyledons for each cultivar of soybeans were compared. The results of adventitious root formation in cultivars are classified as two groups; the first group showed the direct adventitious root formation, and the second group resulted in the callus and adventitious root formation. The cultivars that have much callus formation had less the adventitious root formation. The adventitious root formation in the cotyledonary explants was occured only at the inoculation of adaxial side. When adaxial and abaxial side was inoculated simultaneously, the adventitious roots were formed at the adaxial side. Thus, it suggests that there must be direction to some extent. Starch in the cotyledonary explants were more abundant at the 4 days after induction than at the early stage of the adventitious root formation, but the starch was not observed after 7 days, that the growth stage of adventitious roots.
Effects of Cultural Conditions on the In Vitro Propagation of Plantlets derived from Apical Meristem in Ipomoea batatas L.
Eun, Jong-Seon ; Park, Jong-Suk ; Kim, Young-Seon ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 37~40
DOI : 10.5010/JPB.2002.29.1.037
Sweet potato is a crop vegetatively propagated by vine cuttings, an ineffective method for maintaining pathogene-free stock plants. As an alternative method, single-node cultures of virus-free plantlets derived from apical meristem in sweet potato (cv. Yulmi) was examined. Effective pH range, sugar concentration and nodal order were investigated to establish an in vitro mass propagation system with high quality virus-free stock plantlets to farmhouse. Although the plantlets grew at wide range of pH, the most effective pH of the medium was 4.8 in single-node cultures. High sugar concentration of 60∼80 g/L resulted in increased growth response in shoot length, root length, number of node, leaf area and fresh and dry weight of shoot and root, whereas reducing sugar contents below 6% was showed reduced growth response. The first node including meristem tip was the best for the rapid growth of plantlets and the other nodes also showed a very similar growth response. Uniform plantlet can be obtained massively at the same time by culture of single node except for the first node including meristem tip. In conclusion, the most effective pH range and sugar concentration of medium for the growth of plantlets via single-node cultures was 4.8, 60∼80 g/L respectively. The first node was the best for the rapid propagation of plantlets in nodal order.
Determination of the Period of the Formation and Size of Sieve Element Area and Sieve Pore
Cho, Bong-Heuy ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 41~44
DOI : 10.5010/JPB.2002.29.1.041
During the phloem development from parenchyma cells in a suspension culture of Streptanthus induced sucrose carrier and glucose carrier disappeared. Sieve element area and sieve pore induced suspension culture of Streptanthus were formed almost at the last period of the synthesis of sieve endoplasmic reticulum (SER) and p-protein. The new synthesized cell wall begann to digeste only after the new cell wall was surrounded by SER. The digested region of the cell wall and the formed region of sieve pore were regular comparatively. The completed sieve pore was an oval form, and the outer portion of sieve pore varied, ca 1.2
in longitudinal, 0.8
in tangential, and the inner size of sieve pore was irregular form of a star-like shape. The number of sieve pore between sieve cells was ca 2~7 per
and the sieve pore wall with callose was 0.05
in thickness. The energy for the formation of sieve element area and sieve pore might be supplied by mitochondria near the new cell wall and the role of SER remains to be illucidated.
Effect of Plant Growth Regulators on Callus Induction and Plant Regeration of Farfugium japonica
Lee, Seung-Yeob ; Yoo, Sung-Oh ; Bae, Jong-Hyang ; Lee, Joong-Ho ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 45~49
DOI : 10.5010/JPB.2002.29.1.045
The leaf and petiole segments of Farfugium japonica were cultured to investigate the influence of growth regulators on their callus induction and plant regeneration. The callus induction and growth showed a good response both leaf and petiole on MS media supplemented with 1∼2 mg/L 2,4-D and 1∼2 mg/L BA. Callus induction and growth were more effective in petiole segments than leaf one. The highest percentage of plant regeneration was obtained from 60-day-old calli on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. When subcultured to the same medium for about 60 days, multiple shoots were developed from regenerating callus. The shoots produced roots after transferring to rooting medium containing 0.5 mg/L IAA. The plantlets over 50 mm in height were successfully acclimatized in vermiculite, and the survival rate was over 95%.
Multiple Shoot Formation from Cotyledonary Nodes of Soybean Cultivars
Ha, Keon-Soo ; Han, Tae-Jin ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 51~57
DOI : 10.5010/JPB.2002.29.1.051
For the plant regeneration of soybean (Glycine me L. Merr.), the shoot formation rate, optimal medium and tissue conditions were examined using Korean soybean cultivars. Among the parts of seedling, a node that includes one cotyledon showed the highest shoot formation rate among other tissues. Half-strength B5 medium was more efficient than full strength medium. Formation rates of pair shoots (1 to 2 shooting) were higher in the when benzyl adenine was supplemented. The formation rates of multiple shoots, that is, 4 to 5 in shooting, were high when thidiazuron was supplemented. Multiple shoot was de novo formed in cutting side of cotyledonary node. The effective concentration of thidiazuron for shoot induction treatment was 2 mg/L. Among the 27 cultivars, multiple shoot formation rates were high in the 11 cultivars including `Heugcheongkong, and pair shoot formation rates were high in the 16 cultivars including `Malikong`.
Mass Production of Paclitaxel by Plant Cell Culture
Choi, Hyung-Kyoon ; Son, Joo-Sun ; Na, Gwang-Hwee ; Hong, Seung-Suh ; Park, Yeon-Seung ; Song, Jai-Young ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 59~62
DOI : 10.5010/JPB.2002.29.1.059
Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The paclitaxel-Genexol
-is commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.
Production of Phytol, an ACAT Inhibitor, from Callus Culture of Lettuce (Lactuca sativa L.)
An, Kwang-Hee ; Jang, Tae-O ; Baek, Nam-In ; Kim, Se-Young ;
Journal of Plant Biotechnology, volume 29, issue 1, 2002, Pages 63~68
DOI : 10.5010/JPB.2002.29.1.063
The possibility for mass production of phytol, inhibitory diterpene against ACAT (Acyl-CoA: Cholesterol acyltransferase) was investigated by using callus culture of lettuce. The callus were induced from lettuce cotyledon explants on MS medium containing 0.5 mg.L
NAA after 4 week`s culture. Adventitious roots were formed from the explants on MS medium containing 0.5 mg.L
IBA or 1.0 mg.L
NAA. Adventitious shoots and roots were emerged from the callus when the callus was transferred to MS medium containing auxin alone, or with cytokinin. The plant growth regulators and their concentrations for the organogenesis were 1.0 mg.L
NAA, 0.1 mg.L
BA, 0.5 mg.L
NAA with 0.1 mg.L
kinetin, or 0.5 g.L
2.4-D with 1.0 mg.L
kinetin. Analyses of chlorophyll contents showed that chlorophyll contents were higher in morphogenic calli than in non-morphogenic calli. However, the chemical analyses of gas chromatography indicated that phytol contents were not proportionate to the chlorophyll contents of callus. The content of phytol was higher in callus than in lettuce cotyledon.ledon.