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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 29, Issue 4 - Dec 2002
Volume 29, Issue 3 - Sep 2002
Volume 29, Issue 2 - Jun 2002
Volume 29, Issue 1 - Mar 2002
Selecting the target year
Development of Industrial Transgenic Plants Using Antioxidant Enzyme Genes
Lee, Haeng-Soon ; Kim, Kee-Yeun ; Kwon, Suk-Yoon ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 69~77
DOI : 10.5010/JPB.2002.29.2.069
Oxidative stress derived from reactive oxygen species (ROS) is one of the major damaging factors in plants exposed to environmental stress. In order to develop the platform technology to solve the global food and environmental problems in the 21st century, we focus on the understanding of the antioxidative mechanism in plant cells, the development of oxidative stress-inducible antioxidant genes, and the development of transgenic plants with enhanced tolerance to stress. In this report, we describe our recent results on industrial transgenic plants by the gene manipulation of antioxidant enzymes. Transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts were developed and were evaluated their protection effects against stresses, suggesting that simultaneous overexpression of both SOD and APX in chloroplasts has synergistic effects to overcome the oxidative stress under unfavorable environments. Transgenic tobacco plants expressing a human dehydroascorbate reductase gene in chloroplasts were showed the protection against the oxidative stress in plants. Transgenic cucumber plants expressing high level of SOD in fruits were successfully generated to use the functional cosmetic purpose as a plant bioreactor. In addition, we developed a strong oxidative stress-inducible peroxidase promoter, SWPA2 from sweetpotato (lpomoea batatas). We anticipate that SWPA2 promoter will be biotechnologically useful for the development of transgenic plants with enhanced tolerance to environmental stress and particularly transgenic cell lines engineered to produce key pharmaceutical proteins.
Molecular Cloning and Characterization of Wound-inducible Beta-amyrin Synthase from Soybean
Park, Seong-Whan ; Lee, Jai-Heon ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 79~84
DOI : 10.5010/JPB.2002.29.2.079
Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of wound-induced cDNA, gmwi33 showed high homology with genes encoding
-amyrin synthase. The full length cDNA of gmwi33, designated GmAMS1, is 2416 bp long and contains an open reading frame consisted of 739 amino acids. GmAMS1 protein showed 89% identity with licorice GgbAS1 and 86% identity with pea OSCPSY. In 5 day-old, dark-grown seedlings, the expression of GmAMS1 was most strongly induced by light and weakly induced by methyl jasmonate and by low temperature. However, GmAMS1 was not induced by elicitor or UV-B treatment. Such expression pattern might be closely related with the oxygen-radical scavenging activity of soyasaponin.
Investigation of Transformation Efficiency of Rice Using Agrobacterium tumefaciens and High Transformation of GPAT (glycerol-3-phosphate acyltransferase) Gene Relative to Chilling Tolerance
Seo, Mi-Suk ; Bae, Chang-Hyu ; Choi, Dae-Ock ; Rhim, Seong-Lyul ; Seo, Suk-Chul ; Song, Pill-Soon ; Lee, Hyo-Yeon ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 85~92
DOI : 10.5010/JPB.2002.29.2.085
This study has been focused on improving transformation efficiency of rice using Agrobacterium tumefaciens. We have demonstrated the effect of this system when the GPAT gene related to the cold-resistance was transferred by Agrobacterium tumefaciens in rice. Transformation conditions were modified using intron
-glucuronidase (GUS) expression as a reporter gene in the rice. In this study, mature seed-derived calli of rice (Oruza sativa L. cv. Dongjin) were pre-cultured for 3 days and then infected with Agrobacterium. When this infected calli were cultured in the dark for 10 days on co-cu]lure medium containing 50 mg/L of CaCl
, 30 mg/L of acetosyringone, 2 mg/L of 2,4-D, 120 mg/L of betaine, high GUS expression was observed. In the present transformation system, the efficiency of transformation of GPAT gene was about 54%. Stable integration of GPAT gene into chromosomal DNA was proven by southern blot analysis of genomic DNA isolated from T
progenies. The progenies (T1 generation) derived from primary transformant of 5 lines were segregated with a 3 (resistant) : 1 (sensitive ratio) in medium containing hygromycin. This high frequency transformation system can be used as a useful tool in transformation of another monocotyledon.n.
Introduction of VP6 Gene into Potato Plant by Agrobacterium-mediated Transformation and Analysis of VP6 Expression in Transgenic Potatoes
Youm, Jung-Won ; Jeon, Jae-Heung ; Jung, Jae-Yeol ; Lee, Byoung-Chan ; Kang, Won-Jin ; Kim, Mi-Sun ; Kim, Chul-Joong ; Joung, Hyouk ; Kim, Hyun-Soon ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 93~98
DOI : 10.5010/JPB.2002.29.2.093
A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA
, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.
Isolation of cDNA Encoding Low Temperature-inducible L-asparaginase from Soybean (Glycin max)
Park, Seong-Whan ; Kim, Kee-Young ; Chen, Liang ; Lee, Jai-Heon ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 99~104
DOI : 10.5010/JPB.2002.29.2.099
Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.
Effect of DIF and Cytokinins on In Vitro Growth of `Campbell Early` Crapes (Vitis spp.)
Kim, Seung-Heui ; Kim, Seon-Kyu ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 105~110
DOI : 10.5010/JPB.2002.29.2.105
Effects of difference between day and night temperatures (DIF) and cytokinins on in vitro growth of `Campbell Early` grape plantlets were investigated, Shoot growth was suppressed by -7DIF (18/
). Effects of BA and TDZ were not affected by DIF. 0DIF (25/
) enhanced the growth with increasing cytokinin level. Kinetin at 10
M was the most effective for shoot growth with 87 mm among cytokinins used except for control. Zeatin and kinetin each at 5
M significantly increased shoot growth to respective 162 and 110 mm. In 0DIF, two-fold increase of 162 mm was obtained by 5
M zeatin. In control, no further shoot proliferation was observed regardless of DIF. In 0 and ＋7DIF (25/18
), 4 shoots were observed with 10
M BA. Zeatin, 2iP and kinetin resulted in poor shoot proliferation while BA at 10
M resulted in profuse branching in all DIFs.
Plant Regeneration and Effect of Auxin and Cytokinin on Adventitious Shoot Formation from Seedling Explant of Taraxacum platycarpum
Lee, Mi-Hyun ; Yoon, Eui-Soo ; Jung, Su-Jin ; Bae, Ki-Hwa ; Seo, Jin-Wook ; Choi, Youg-Eui ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 111~115
DOI : 10.5010/JPB.2002.29.2.111
Taraxacum platycarpum has been used as a medicinal plant. We investigated optimal condition for efficient plant regeneration through adventitious shoot formation on medium with various kinds of growth regulators. Adventitious shoot formation was achieved when cytokinin was used alone. Shoot formation was higher on MS medium containing 2 mg/L BAP compared to that with 2 mg/L kinetin and 2 mg/L 2-ip. Among root, hypocotyl and cotyledon, roots were the best explant for the adventitious shoot induction. Adventitious shoot formation from roots declined markedly by the combination of both 0.1 mg/L NAA and 2 mg/L BAP, while shoot formation from cotyledons was stimulated by the same combination. Root formation from the regenerated shoots was achieved on 1/3MS medium containing 0.2 mg/L NAA. Regenerated plantlets was acclimatized and transplanted to the soil, showing 100% survival.
Micropropagation of Juvenile and Mature Tree of Corylopsis coreana by Axillary Bud Culture
Moon, Heung-Kyu ; Noh, Eun-Woon ; Ha, Yoo-Mi ; Shim, Kyung-Ku ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 117~121
DOI : 10.5010/JPB.2002.29.2.117
We have developed an in vitro micropropagation system via shoot formation from axillary buds using nodal segments of Corylopsis coreana. Explants from both juvenile tree (one-year-old greenhouse stock seedlings) and mature tree (ten-years-old tree in nursery) were compared with regard to propagation efficiency. Combined treatment of both BA and zeatin were effective on shoot proliferation since the best result was obtained on MS medium supplemented with 0.5∼3.0 mg/L zeatin and 0.2 mg/L BA. Generally, juvenile explants were better in both shoot proliferation and growth than mature explants. However, as the duration of in vitro culture was proceed to 6 months, explants from mature tree also produced three shoots per explant. Distinctive differences in rooting and adaptability to soil of shoots obtained from mother trees. Whereas shoots originated from juvenile explants rooted as high as 97%, those from adult explants showed 62% rooting. Similar result was also observed in soil acclimatization. The plantlets derived from juvenile plants survived 67%, while only 48% of those from adult trees survived. The results showed a possibility of the micropropagation of Corylopsis coreana through shoot formation from axillary buds. In addition, the advance of the research still remain to enhance the frequency of acclimatization of plantlets from mature trees for practical application.
Effect of Cytokinins on in Vitro Growth of Grapes (Vitis spp.)
Kim, Seung-Heui ; Kim, Seon-Kyu ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 123~127
DOI : 10.5010/JPB.2002.29.2.123
Effect of cytokinins (BA, TDZ, zeatin, 2iP, and kinetin) applied either singly or in combination on in vitro growth of two grape cultivars (`Cabernet Sauvignon` and `Campbell Early`) was investigated as a serial work for mass production of grapevine nursery stocks. In single treatment, shoot growth of two cultivars was most favorable in control. Shoot proliferation was satisfactory with 10
M BA regardless of cultivars and cytokinin combinations, followed by TDZ. Other treatments resulted in very poor or no branching. Total explants ready for subculture produced by 10
M BA outnumbered those by other treatments. TDZ was also effective. TDZ significantly increased the fresh weight and callus formation while shoot growth was unsatisfactory. Shoot growth response of two cultivars in combined treatments was also most favorable in control as was in single treatments. When TDZ was combined with zeatin, 2iP, and kinetin which failed to induce branching, proliferous branching was induced though the shoot number was behind that of single treatments of BA and TDZ. TDZ was very effective for total number of explants and fresh weight, showing 10-fold increase.
Characteristics on the Field Growth of Plantlets Regenerated from Leaf Segment Cultures of Boxthorn (Lycium chinense Mill.)
Kim, Dong-Chan ; Chung, Hae-Joon ; Min, Byung-Hoon ; Yang, Deok-Chun ; Kim, Su-Dong ; Lee, Bong-Chun ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 129~134
DOI : 10.5010/JPB.2002.29.2.129
194 plantlets regenerated from leaf explants of boxthorn `Cheonyang` were cultivated to investigate their morphological characteristics in the field for 2 years. Based on the morphology of leaves, 66.1% of them had elliptical type leaf, the same as that of mother plants, while 22.2% in oval type, 7.2% in obovate type, 2.6% in long-obvate type and 2.1% in lanceolate type. They were classified to 4 groups; group A was selected with both high fruit size and fruit yield, group B with only high fruit size, group C with larger or thicken leaf, and group D with multiple brenches. In comparision of production efficiency between the selected groups and mother plants, group A (99741, 99781, 99854, 99870 and 99886) were longer (2.1 to 2.7 mm) in length of fruits and higher in fruit production (15 to 30%) as compared to mother plants. Croup D (99797 and 99892) was higher in leaf production (7.2%) as compared to boxthorn `CL1-48`, which is the highest in leaf production among boxthorn veriaties.
Screening of Soybean Recombinant Inbred Lines for High Competence Somatic Embryogenesis
Choi, Pil-Son ; Takao Komatsuda ; Kim, Min-Hoon ; Choi, Kyu-Myeong ; Choi, Dong-Woog ; Liu, Jang-Ryol ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 135~138
DOI : 10.5010/JPB.2002.29.2.135
Cotyledonary explants from immature zygotic embryos of each 85 recombinant inbred lines (RILs) were cultured on medium containing MS salts, B5 vitamins, 40 mg
2,4-dichlorophenoxyacetic acid and 30 g
sucrose. Frequency of somatic embryo formation on cotyledonary explants showed in thirty-six lines(＜10%), in thirty-seven lines (11~49%), in nine lines (50~89%), and in three lines(>90%), respectively, The highest frequency (up to 90%) and number (6.36 per cotyledon) of somatic embryos were obtained from lines of KM1010, KM1032 and KM1064. Primary somatic embryos produced from three lines produced numerous secondary somatic embryos on the surfaces, which were subcultured for over one year. Upon transfer to maturation and conversion medium (Komatsuda, 1992), somatic embryos converted to plantlets at a frequency of approximately 25%.
Metabolic Engineering of the Brassinosteroid Biosynthetic Pathways
Lee, Mi-Ock ; Song, Ki-Hong ; Lee, Hyun-Kyung ; Jung, Ji-Yoon ; Choe, Vit-Nary ; Choe, Sung-Hwa ;
Journal of Plant Biotechnology, volume 29, issue 2, 2002, Pages 139~144
DOI : 10.5010/JPB.2002.29.2.139
Sterols play two major roles in plants: a bulk component in biological membranes and precursors of plant steroid hormones. Physiological effects of plant steroids, brassinosteroids (BRs), include cell elongation, cell division, stress tolerance, and senescence acceleration. Arabidopsis mutants that carry genetic defects in BR biosynthesis or its signaling display characteristic phenotypes, such as short robust inflorescences, dark-green round leaves, and sterility. Currently there are more than 100 dwarf mutants representing 7 genetic loci in Arabidopsis. Mutants of 6 loci, dwf1/dim1/cbb1, cpd/dwf3, dwf4, dwf5, det2/dwf6, dwf7 are rescued by exogenous application of BRs, whereas bri1/dwf2 shares phenotypes with the above 6 loci but are resistant to BRs. These suggest that the 6 loci are defective in BR biosynthesis, and the one locus is in BR signaling. Biochemical analyses, such as intermediate feeding tests, examining the levels of endogenous BR, and molecular cloning of the genes revealed that dwf7, dwf5, and dwf1 are defective in the three consecutive steps of sterol biosynthesis, from episterol to campesterol via 5-dehydroepisterol. Similarly, det2/dwf6, dwf4, and cpd /dwf3 were shown to be blocked in D
reduction, 22a-hydroxylation, and 23 a-hydroxylation, respectively. A signaling mutant bril/dwf2 carries mutations in a Leucine-rich repeat receptor kinase. Interestingly, the bri1 mutant was shown to accumulate significant amount of BRs, suggesting that signaling and biosynthesis are dynamically coupled in Arabidopsis. Thus it is likely that transgenic plants over-expressing the rate-limiting step enzyme DWF4 as well as blocking its use by BRIl could dramatically increase the biosynthetic yield of BRs. When applied industrially, BRs will boost new sector of plant biotechnology because of its potential use as a precursor of human steroid hormones, a novel lead compound for cholesterol-lowering effects, and a various application in plant protection.