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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 29, Issue 4 - Dec 2002
Volume 29, Issue 3 - Sep 2002
Volume 29, Issue 2 - Jun 2002
Volume 29, Issue 1 - Mar 2002
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Prospects for Plant Biotechnology and Bioindustry in the 21s1 Century: Paradigm Shift Driven by Genomics
Liu, Jang-Ryol ; Choi, Dong-Woog ; Chung, Hwa-Jee ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 145~150
DOI : 10.5010/JPB.2002.29.3.145
Biotechnology in the 21st century will be driven by three emerging technologies: genomics, high-throughput biology, and bioinformatics. These technologies are complementary to one another. A large number of economically important crops are currently subjected to whole genome sequencing. Functional genomics for determining the functions of the genes comprising the given plant genome is under progress by using various means including phenotyping data from transgenic mutants, gene expression profiling data from DNA microarrays, and metabolic profiling data from LC/mass analysis. The aim of plant molecular breeding is shifting from introducing agronomic traits such as herbicide and insect resistance to introducing quality traits such as healthful oils and proteins, which will lead to improved and nutritional food and feed products. Plant molecular breeding is also expected to aim to develop crops for producing human therapeutic and industrial proteins.
Construction of a Genetic Linkage Map in Radish(Raphanus sativus L.) Using RAPD Markers
Ahn, Choon-Hee ; Choi, Su-Ryun ; Lim, Yong-Pyo ; Chung, Hae-Joon ; Yae, Byeong-Woo ; Yoon, Wha-Mo ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 151~159
DOI : 10.5010/JPB.2002.29.3.151
Genetic map and molecular marker have a great importance in improving and facilitating crop breeding program as well as in genome analysis and map-based cloning of genes representing desirable characters. This study aimed at developing RAPD markers and constructing a genetic linkage map using 82 BC
individuals originated from the cross between `835` and B
in radish (Raphanus sativus L.). One of the parents for genetic linkage map construction, `835`(P
) of egg type is susceptible to Fusarium wilt and have medium resistance to virus infection and the other parent, B
) of round type, is susceptible to Fusarium wilt and virus, Screening of 394 RAPD primers in BC
) population resulted in selecting 128 polymorphic markers which displayed 1:1 segregation pattern. Two markers failed to display 1:1 segregation and showed the segregation ratio skewed to maternal genotype. Selected markers were categorized into 14 linkage group based on LOD score represented by MAPMAKER/EXP program. Five groups composed of single marker among them were excluded from the linkage map, and consequently, the remaining groups are well matched with the number of radish chromosome (n＝9). The linkage map constructed with 128 markers covers 1,688.3 cM and the average distance between markers was 13.8 cM. For developing STS marker, we determined the partial nucleotide sequence of OPE10 marker at both ends and designed a oligonucleotide primer pair based on this sequence. STS PCR using the primer pair displayed a single, clear band of which segregation is perfectly matched with that of OPE10 marker. This implies that RAPD markers could readily convert into clear and reliable STS markers.
Proteome Data Analysis of Hairy Root of Panax ginseng : Use of Expressed Sequence Tag Data of Ginseng for the Protein Identification
Kwon, Kyung-Hoon ; Kim, Seung-Il ; Kim, Kyung-Wook ; Kim, Eun-A ; Cho, Kun ; Kim, Jin-Young ; Kim, Young-Hwan ; Yang, Deok-Chun ; Hur, Cheol-Goo ; Yoo, Jong-Shin ; Park, Young-Mok ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 161~170
DOI : 10.5010/JPB.2002.29.3.161
For the hairy root of Panax ginseng, we have got mass spectrums from MALDI/TOF/MS analysis and Tandem mass spectrums from ESI/Q-TOF/MS analysis. While mass spectrum provides the molecular weights of peptide fragments digested by protease such as trypsin, tandem mass spectrum produces amino acid sequence of digested peptides. Each amino acid sequences can be a query sequence in BLAST search to identify proteins. For the specimens of animals or plants of which genome sequences were known, we can easily identify expressed proteins from mass spectrums with high accuracy. However, for the other specimens such as ginseng, it is difficult to identify proteins with accuracy since all the protein sequences are not available yet. Here we compared the mass spectrums and the peptide amino acid sequences with ginseng expressed sequence tag (EST) DB. The matched EST sequence was used as a query in BLAST search for protein identification. They could offer the correct protein information by the sequence alignment with EST sequences. 90% of peptide sequences of ESI/Q-TOF/MS are matched with EST sequences. Comparing 68% matches of the same sequences with the nr database of NCBI, we got more matches by 22% from ginseng EST sequence search. In case of peptide mass fingerprinting from MALDI/TOF/MS, only about 19% (9 proteins of 47 spots) among peptide matches from nr DB were correlated with ginseng EST DB. From these results, we suggest that amino acid sequencing using tandem mass spectrum analysis may be necessary for protein identification in ginseng proteome analysis.
Commercial Production of Seed Garlic by Tissue Culture Technique
Nam, Sang-Il ; Park, Ju-Hyun ; Choi, Jong-In ; Kwon, Ki-Seok ; Uhm, Jeong-Sik ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 171~177
DOI : 10.5010/JPB.2002.29.3.171
We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM`s tissue culture technique is called as `Multiple shoot propagation technique` This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots (15/sup 10//year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above 50%) and also can enhance the product quality. Consequently, we estimated that TYM`s seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.
Effects of Explant Parts and Plant Growth Regulators on the in vitro Propagation of Lycoris squamigera
Eun, Jong-Seon ; Kim, Young-Seon ; Park, Jong-Suk ; JIN, Song Nan ; CAO, Hounan ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 179~183
DOI : 10.5010/JPB.2002.29.3.179
This study was carried out to investigate the influence of medium composition for in vitro mass propagation of Lycoris squamigera Max. After the disks of short stems, segments of leaf within bulb and scale were cultured on MS basal medium supplemented with various plant growth regulators, they were examined for the extent of callus formation, shoot and root regeneration. In the culture of stem disks, adventitious shoots were regenerated from the basal tissue of bulb scales, and combined medium of 1.0 mg/L 2,4-D or NAA＋2.0 mg/L BA or kinetin showed the the best response and 4∼6 shoots per explant formed. In the culture of leaf segments within bulbs, both MS medium supplemented with 1.0 mg/L NAA＋2.0 mg/L TDZ and with 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA were produced callus profusely on the base of leaf tissue and 3∼6 shoots were regenerated per explant. In the scale segments culture, calli were produced on the basal tissue on medium with 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA. The best result were shown on MS medium with 1.0 mg/L NAA＋2.0 mg/L TDZ, and 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA. Maximum number of regenerated shoots was up to 10∼12. Adventitious root formation from explants were formed profusely on MS medium with 1.0 mg/L NAA＋2.0 mg/L kinetin. The most desirable method for mass propagation of plantlets was the shoot regeneration from scale segments then subsequently subcultured on medium for rooting.
Somatic Embryogenesis and Plant Regeneration in Mature Seed Cultures and Seed-Derived Embryogenic Suspension Cultures of Yuzu
Min, Sung-Ran ; Choi, Myung-Suk ; Jeong, Won-Joong ; Liu, Jang-Ryol ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 185~188
DOI : 10.5010/JPB.2002.29.3.185
Off-white, friable embryogenic calluses were formed on the internal integument of mature seeds of yuzu (Citrus junos) cultured on Murashige and Skoog`s basal medium at a frequency of 1.2%. Embryogenic calluses were proliferated when cultured on medium with 1 mg/L 2,4-D. Upon transfer to medium with 0.1 mg/L kinetin, embryogenic calluses produced numerous somatic embryos. Embryogenic suspension cultures were established by placing embryogenic calluses into liquid medium with 1 mg/L 2,4-D. When plated onto medium with 0.5 mg/L ABA, embryogenic cells developed into somatic embryos at a high frequency, and then regenerated into plantlets. Plantlets were successfully transplanted to potting soil and grown in a greenhouse.
Plant Regeneration of Major Cultivars of Sweetpotato (Ipomoea batatas) in Korea via Somatic Embryogenesis
Kwon, Eun-Jeong ; Kwon, Suk-Yoon ; Kim, Moon-Za ; Lee, Joon-Seol ; Ahn, Young-Sup ; Jeong, Byeong-Choon ; Kwak, Sang-Soo ; Lee, Haeng-Soon ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 189~192
DOI : 10.5010/JPB.2002.29.3.189
An efficient plant regeneration system of major cultivars of sweetpotato (Ipomoea batatas (L.) Lam.) in Korea via somatic embryogenesis was established. Embryogenic calli were formed from shoot apical meristems of sweetpotato cultivars when cultured on LS medium supplemented with 1 mg/L auxin (2,4-D, picloram, dicamba). Among three kinds of auxin, 1 mg/L 2,4-D showed the highest embryogenic calli induction rate. After 4 weeks of cultures on LS medium supplemented with 1 mg/L 2,4-D, embryogenic calli induction rates of Sinhwangmi, Zami, Yulmi, and White Star were 86%, 78%, 76%, and 80%, respectively. Upon transfer onto LS basal medium, most of somatic embryos developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to mature plants in a greenhouse.
Effect of Phenylacetic Acid (PAA) on Embryo Formation in Anther and Microspore Culture of Paeonia lactiflora
Kwon, Yong-Sham ; Shin, Young-Ae ; Sohn, Jae-Keun ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 193~198
DOI : 10.5010/JPB.2002.29.3.193
The objective of this study was to determine the effects of phenylacetic acid (PAA) on embryo production in anther and microspore culture of herbaceous peony (Paeonia lactiflora Pall.). The anthers of herbaceous peony were cultured on MS medium with 0 to 100 mg/L PAA according to two-step culture method. The ruptured anthers were transferred onto embryo formation medium without growth regulators. The MS medium with 2 mg/L PAA was effective in enhancing of direct embryogenesis and producing of normal embryo with two cotyledons from the cultured anthers. However, the increase of PAA concentration more than 5 mg/L PAA inhibited the embryo formation and promoted to callus formation from the anthers. The PAA affects significantly on the division of microspore and embryo formation in shed pollen culture and the best result was obtained from a medium supplement with 2 mg/L PAA. The preculture of anther for 10 days on solid medium with 2 mg/L PAA was effective for embryo formation from shed microspore of herbaceous peony.
Metabolic Engineering of Medicinal Plants tov Tropane Alkaloid Production
Yun, Dae-Jin ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 199~207
DOI : 10.5010/JPB.2002.29.3.199
The tropane alkaloids hyoscyamine (its racemic form being atropine) and scopolamine are used medicinally as anticholinergic agents that act on the parasympathetic nerve system. Because they differ in their actions on the central nervous system, currently there is a 10-fold higher commercial demand for scopolamine, in the N-butylbromide form, than there is for hyoscyamine and atropine combined. Several solanaceous species have been used as the commercial sources of these alkaloids, but the scopolamine contents in these plants often are much lower than those of hyoscyamine. For this reason there has been long-standing interest in increasing the scopolamine contents of cultivated medicinal plants. Naturally occurring and artificial interspecific hybrids of Duboisia have high scopolamine contents and are cultivated as a commercial source of scopolamine in Australia and other countries. Anther culture combined with conventional interspecific hybridization also has been used to breed high scopolamine-containing plants in the genera Datura and Hyoscyamus, but without much success. The use of recombinant DNA technology for the manipulation of metabolic processes in cells promises to provide important contributions to basic science, agriculture, and medicine. In this review, I introduce on the enzymes and genes involved in tropane alkaloid biosynthesis and current progress in metabolic engineering approaches for tropane alkaloid, especially scopolamine, production.
Growth Factors Affecting to Kalosaponins Contents of Kalopanax pictus Nakai
Lee, Cheol-Ho ; Jo, Dong-Kwang ; Lee, Kap-Yeon ; Kwon, Ki-Won ; Choi, Myung-Suk ;
Journal of Plant Biotechnology, volume 29, issue 3, 2002, Pages 209~215
DOI : 10.5010/JPB.2002.29.3.209
Four kinds of kalosaponins from inner bark of Kalopanax pictus Nakai as a medicinal and edible timber tree species growing in Korea was analyzed by HPLC. A considerable range of variation in the contents was observed among population. The kalosaponins contents in inner bark from each population were the highest in the Mt. Barwang (30.37 mg/g on the dry weight basis) followed by Mt. Gariwang, Hanra II, Mangun, Paltan, and Hanra II population. Growth factors that might have influenced the contents of the kalosaponins were also examined. As both analysis results of partial correlation and multiple regression, the weight of inner bark and tree age were influenced the kalosaponin O contents. The kalosaponin B contents was influenced by both the weight of inner layer bark and annual mean DBH increment. The contents of kalosaponins appeared to be closely related to the inner bark characteristics.