Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 30, Issue 4 - Dec 2003
Volume 30, Issue 3 - Sep 2003
Volume 30, Issue 2 - Jun 2003
Volume 30, Issue 1 - Mar 2003
Selecting the target year
Current Research Status on the Development of Genetically Modified Plants in Korea
Lee, Shin-Woo ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 1~6
DOI : 10.5010/JPB.2003.30.1.001
In an attempt to evaluate the current research status of genetically modified (GM) plants, the scientific research publications in Korea as well as in international SCI journals were screened. About 190 research articles related to the development of GM plants were searched from 10 different domestic journals in the last 12 years (Jan. 1990 to Sept. 2002), The researches in 65 articles were carried out with tobacco plant, 20 with rice, 19 with potatoes, and less then 9 articles from each other plant species, respectively, In total, 38 different plant species were being subjected for the development of GM plants. In particular, there was only one article for each major staple grains such as wheat, barley, soybean, and maize. In more than 47% of total published articles, scientists mainly focused on the basic research such as developing transformation system (46 articles), gene expression study in transgenic plants (34), and vector constructions (10). In addition, 28 articles which main authors are Korean scientists were searched from 11 different international SCI journals. Again, major plants for GM research were tobacco (10) and rice (7). More than 50% of published articles were focused on the basic research, gene expression study with transgenic plants (16). The publications on the research of disease-resistant plants were 7 articles, 3 for the development of stress-resistant and 2 for the herbicide-resistant plants, respectively. It is believed that the last 10 year`s investment through government organizations has just strengthen the capacity for the next big stride on agricultural biotechnology in Korea.
Karyotype Analysis and Physical Mapping of rDNAs in Diploid Nicotiana plumbaginifolia
Cho, Hye-Gyoung ; Koo, Dal-Hoe ; Kim, Soo-Young ; Bang, Jae-Wook ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 7~11
DOI : 10.5010/JPB.2003.30.1.007
Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using FISH (fluorescence in situ hybridization) technique were carried out in Nicotiana plumbaginifolia. Somatic chromosome number of N. plumbaginifolia was 2n
Distinct Spatio-temporal Expression Patterns of Patatin Promoter-GUS Gene Fusion in Transgenic Potato Microtubers
Youm, Jung-Won ; Kim, Mi-Sun ; Lee, Byoung-Chan ; Kang, Won-Jin ; Jeon, Jae-Heung ; Joung, Hyouk ; Kim, Hyun-Soon ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 13~18
DOI : 10.5010/JPB.2003.30.1.013
This study was carried out to investigate the expression patterns of foreign gene that controlled by tuber-specific patatin promoter in transgenic potatoes. Potato leaf disc cultured in vitro were transformed by the Agrobacterium strain LBA4404 containing pBl121 or pATGUS from potato cv. Desiree. In order to select the transgenic lines, gene-specific primers deduced from the NPTII were synthesized and used for polymerase chain reaction. The down part of the putative transgenic potatoes was transplanted weekly onto sucrose-enriched medium to accelerate the microtuber formation. RNA gel blot analysis was performed on the total RNAs obtained from tuber that had been harvested at a week interval. Also, histochemical assay was observed in the explants transformed with either pBI121 or pATGUS. Results showed that the transgenic plant containing pATGUS expressed GUS transcripts mainly at the tuber, not in stem, with the highest expression level in 5 weeks-grown microtubers. In contrast to pATGUS plants, the transformed plants with pBI121 showed an equal expression pattern throughout the whole developing stages. Consistent with RNA gel blot analysis, histochemical GUS staining and enzyme activity exhibited pATGUS transcripts were at the highest level in 5 weeks cultures. From these results, we suggest that the best stage to analyze the foreign gene introduced by patatin promoter into potato plants is at 5 weeks cultures after tuber formation.
Selection and Agronomic Traits of Radiation-induced Variants in Rice
Lee, In-Sok ; Kim, Dong-Sub ; Lee, Sang-Jae ; Song, Hi-Sub ; Lim, Yong-Pyo ; Lee, Young-Il ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 19~25
DOI : 10.5010/JPB.2003.30.1.019
Radiation technique in agriculture was initiated to develop mutant rice. Seeds of Daechungbyeo rice were irradiated with 250 Gy gamma ray for the purpose of inducing and selecting rice variants. Some quantitative traits of the variants in M
generation were evaluated and RAPD analysis was carried out. Variants showed a wider range of agronomic characteristics in both a positive and a negative direction compared with their original variety. The new mutants were characterized by an increased or decreased in plant height, lodging resistance and shorter panicle. RAPD analysis showed that polymorphic bands were presented in most of the primers. In comparison with the original variety, variants were classified into four groups through UPGMA analysis. Among mutants no. 91, 139, 140 and 141 was ranked as salt tolerance and the proline content of these mutants was more increased than that of original variety. The lines of 139, 140 and 141 had the highest genetic distance as compared to original variety in the dendrogram. It is expected that such variants will be useful not only for studying molecular genetics but also for breeding research and genetic analysis.s.
Effects of Hormone and Several Factors on the Regeneration and Transformation rate of Potato Cultivars Bred in Korea
Yi, Jung-Yoon ; Seo, Hyo-Won ; Cho, Ji-Hong ; Lee, Shin-Woo ; Yun, Han-Dae ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 27~33
DOI : 10.5010/JPB.2003.30.1.027
The optimal condition of in vitro regeneration and transformation were investigated for newly bred potato varieties in Korea. Leaf and internodal stem tissues of `Chubak`, `Namsuh`, `Jasim`, `Jopung` and `Jowon` were used to investigate the influence of growth regulator on regeneration efficiency The effect of phenolic compound acetosyringone on gene transformation efficiency was also investigated. Leaf tissue of `Jowon` and internodal stem tissues of `Jopung` were showed high regeneration efficiency on M5 medium supplemented with 0.1 mg/L GA₃, 2.0 mg/L Zeatin and 0.01 mg/L NAA. The other three potato cultivars were showed low regeneration efficiency less than 25%. The effect of acetosyringone on Agrobacterium-mediated gene transformation with leaf and internodal stem tissues were noticeable. By adding the 75 μM of acetosyringone during the Agrobacterium innoculation, the transformation efficiency was increased up to 1.5∼4.0 fold compare to non-treated control. In case of `Jowon` the transformation efficiency was 87.9% in leaf tissue and `Jopung` was 68.4% in internodal stem tissues.
Increase of Larger-sized Pollen Number by Gametocide and Callus Induction in Anther Culture of Zoysia japonica Steud.
Cho, Moon-Soo ; Juang, Ue-Dong ; Ye, Byong-Kwea ; Ahn, Byung-Joon ; Choi, Joon-Soo ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 35~40
DOI : 10.5010/JPB.2003.30.1.035
In this study we investigated the effect of gametocides on the number of larger-sized pollen in anther, and also induced callus from the anther culture of Zoysia japonica Steud. Before culturing, we have observed pollens in anther through fluorescence and electron microscopes to know pollen dimorphism. There were two types of pollens observed. One type (30-36
in diameter) consisted of vacuolated, larger-sized pollens and the other (15-20
in diameter) smaller-sized ones with dense cytoplasm and plenty of amyloplasts. Within few hours, all the smaller-sized pollens were dead, while larger-sized ones were viable for one or two days. To induct larger-sized pollens, various gametocides were leaf-sprayed on three booting stages cultured under 4
(day/night) before anther culturing. Number of these larger pollens were few (less than 1%) in anther without spraying gametocides. GA
increased the number of larger-sized pollens when applied at mid-booting stage. GA
with 50 mg/L treatment caused the highest percentage (25.4%) of the larger-sized pollen. Anthers with GA
treatment were only produced calli on AA medium (modified B
+8.0 mg/L 2,4-D ＋0.2 mg/L kinetin), but callus formation was quite low (less than 1%).).
Effects of Light, Temperature, and Sucrose on Plant Regeneration from the Flower Organ Explant in Iris ensata
Yoon, In-Kyung ; Koh, Jae-Chul ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 41~45
DOI : 10.5010/JPB.2003.30.1.041
A study was under taken to investigate the appropriate explant sources of flower organ and suitable cultural conditions such as light, temperature, and sucrose in plant regeneration of Iris ensata culture. Explants of perianth, ovary, pedicel, and peduncle of Iris ensata were cultured at different daylength (0, 8, 16, 24 hour), different temperatures (10, 15, 25, 3
), and sucrose concentrations (1, 3, 6, 9%) on MS medium. Formation of adventitious roots from explants of Iris ensata was effective in the dark, while that of adventitous shoots was effective in the light. The optimum daylength for young plant regeneration was 16 hours. The optimum temperature for shoot formation of Iris ensata explants was
but the formation at 10 and 15
was ineffective. Especi-ally, perianth and ovary was effective in shoot formation from flower organ expants. T-he optimum concentration of sucrose for shoots and roots formation of Iris ensata explants was 3 and 6%, respectively.
Regeneration and Acclimatization of Plants Derived from Anther Cultures in Carrot (Daucus carota L.)
Cho, Moon-Soo ; Juang, Ue-Dong ; Park, Sang-Gyu ; Park, Yong ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 47~52
DOI : 10.5010/JPB.2003.30.1.047
Anthers from several lines of carrot (Daucus carota L.) were plated on the semi-solid B
, basal medium supplemented with 2,4-D and NAA at two concentrations, 1.0 and 2.0 mg/L plus 0.2 mg/L BAP (benzylaminop-urine). Anthers of the most lines on the B
basal medium with 2,4-D showed higher percentages of callus formation than those with NAA. Particularly, in line 45477, highest percentages of callus formation (50%) were observed on B
medium with 1.0 mg/L 2,4-D plus 0.2 mg/L BAP. With 1.0 mg/L 2,4-D, two months was sufficient for initiation of callus development. Calli were regenerated into plantlets through embryogenesis onto regeneration medium without any growth regulators. When callus showing yellowish and soft structure was cultured, it yielded green plants at high regeneration rates, The response of anthers in callus induction and plant regeneration was different among lines investigated. Optimal callus induction and plant regeneration could be obtained through manipulating the concentration of growth regulators. Plantlets after transfer to perlite were grown successfully in greenhouse conditions. Anther culture of carrot will be used as a useful breeding tool in future.
Plant Regeneration Through Adventitious Bud Formation and Callus Induction from Scales of Lilium lancifolium Thunb.
Nam, Sang-Wook ; Kim, Hei-Young ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 53~58
DOI : 10.5010/JPB.2003.30.1.053
This study was performed to investigate the effects of various media compositions in regeneration of Lilium lancifolium. The adventitious bud initiation from microscale was the best on MS medium supplemented with BAP 1.0 mg/L and NAA 0.1 mg/L after 4 weeks of culture. However, from bulbscales, adventitious bud initiation was the best in dark condition on MS medium supplemented with BAP 0.5 mg/L and NAA 0.1 mg/L. On the other hand, callus induction was found to be the best from the microscales incubated in complete dark condition for 8 weeks on MS medium supplemented with 2,4-D 1.0 mg/L and BAP 0.1 mg/L. The highest plantlet regeneration from callus was obtained after incubation in the light condition for 8 weeks on MS medium supplemented with NAA 0.5 mg/L and BAP 0.1 mg/L. Rooting of shoots was obtained easily on MS medium and the plantlets were transferred to soil pots after 8 weeks. The chromosome analysis of the root tip cells was revealed that the callus-derived plantlets had normal chromosome number, 2n
In Vitro Propagation of Zantedeschia spp. through Shoot Tip Culture
Han, Bong-Hee ; Cho, Hae-Ryong ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 59~63
DOI : 10.5010/JPB.2003.30.1.059
This experiment was conducted to propagate Zantedeschia spp. in vitro. The frequency of adventitious bud clusters (ABC) formation from shoot tips in Z. `Best Gold` was high at more than 65% on media with 2.0∼5.0 mg/L BA or 0.1∼1.0 mg/L thidiazuron. The highest formation rate of ABC (75%) was obtained on medium containing 2.0 mg/L BA. Comparing to treatment of BA alone, combined one of BA and NAA did not stimulate the formation of ABC and the shoot regeneration from shoot tips. The proliferation of ABC from sections (0.7∼1.0 cm) of ABC occurred effective on medium with 2.0 mg/L BA. Shoots developed from the sections (0.7∼1.0 cm) of ABC grew and rooted favorably on media containing 1.0∼2,0 mg/L IBA. The shoots were multiplicated effectively on medium with 0.5 mg/L thidiazuron in Z. `Childsiana`, on medium with 3.0 mg/L BA in 2. `Golden Affair`, and on medium with 5.0∼10.0 mg/L BA in Z. `Pacific Pink`.
Optimum Condition for Mass Culture of Hairy Roots from Artemisia sylvatica MAX
Shin, Sun-Hee ; Yang, Deok-Cho ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 65~71
DOI : 10.5010/JPB.2003.30.1.065
This research aims the production of anti-tumor substances through in vitro culture of hairy roots transformed by Agrobacterium rhizogenes in Artemisia sylvatica MAX and the effect of culture conditions on optimum growth of hairy roots. We investigated the optimum medium, pH, carbon source, sucrose, light, Fe and polyamine conditions of various lines of hairy roots (NK3, NK4, YX. NK3-10) induced from Artemisia sylvatica to increase the optimum growth of hairy roots. MS medium was the best for optimum growth of hairy root clone, NK3-S10. The optimum culture period was 4 weeks for NK3-S10. The optimum sucrose concentration was 3.5%. The optimum concentration of FeSO
, spermine and spermidine was 0.1 mM, 10 mM and 100 mM, respectively,
Plant Regeneration and Mutagenesis from Organogenic Callus of Dianthus Distributed in Gangwon Province
Chang, Mi-Young ; Hong, Sung-Won ; Kim, Joon-Chul ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 73~80
DOI : 10.5010/JPB.2003.30.1.073
Useful Dianthus species were collected and selected from two native and seven foreign species distributed in Gangwon province. For in vitro breeding,. callus was induced from the explants of apical meristem, leaf, stem and the in vitro adventitious shoots on MS basal medium with 2.0 mg/L 2,4-D and 0.5 mg/L BA at 27
under continuous light. After 3 weeks of culture, calli initiated the most highly from the leaf explants of D. chinensis Organogenic calli were able to be selected from the adventitious shoot-derived calli. For shoot regeneration, these organogenic calli were cultured on MS medium with the combination of 0.1 mg/L NAA＋1.0 mg/L BA under continuous light. Multiple shoots were proliferated with low frequency (about 30%) from those adventitious shootderived calli. Also, shoots initiated directly from the adventitious shoot explants without callus formation at high frequency of 52% when cultured on N6 medium containing 0.1 mg/L NAA and 1.0 mg/L BA in D. gratianopol. Multiple shoots and plantlets grew well and rooted on MS medium supplemented with 0.1 mg/L NAA. Regenerants with well-developed roots were transferred to 8-cm pots containing vermiculite at 85% relative humidity and 27
These plantlets were acclimatized in artificial soil mixture and transferred to the greenhouse for flowering with normal phenotypes. M28 Mutant line was selected with white flowers from 0.03M EMS-treated organogenic calli derived from in vitro adventitious shoot explants of D. chinensis and set seeds.
Carotenoids Biosynthesis and Their Metabolic Engineering in Plants
Ha, Sun-Hwa ; Kim, Jung-Bong ; Park, Jong-Sug ; Ryu, Tae-Hun ; Kim, Kyung-Hwan ; Hahn, Bum-Soo ; Kim, Jong-Bum ; Kim, Youg-Hwan ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 81~95
DOI : 10.5010/JPB.2003.30.1.081
Carotenoids are synthesized from the plastidic glyceraldehyde-3-phosphate (GAP)/pyruvate pathway in isoprenoids biosynthetic system of plants. They play a crucial role in light harvesting, work as photoprotective agents in photosynthesis of nature, and are also responsible for the red, orange and yellow colors of fruits and flowers in plants. In addition to biological actions of carotenoids as antioxidants and natural pigments, they are essential components of human diet as a source of vitamin A. It has been also suggested that some kinds of carotenoids might provide protection against cancer and heart disease as human medicines. In this article, we review the commercial applications on the basis of biological functions of carotenoids, summarize the studies of genes involved in the carotenoid biosynthetic pathway, and introduce recent results achieved in metabolic engineering of carotenoids. This effort for understanding the carotenoids metabolism will make us to increase the total carotenoid contents of crop plants, direct the carotenoid biosynthetic machinery towards other useful carotenoids, and produce a new array of carotenoids by further metabolizing the new precursors that are created when one or two key enzymes in carotenoid biosynthetic pathway are exchanged through gene manipulation in the near future.
Production of hGM-CSF from Cell Suspension Culture of Transformed Lettuce Using Agrobacterium-mediated Transformation System
Kim, Young-Sook ; Kim, Mi-Young ; Kwon, Tae-Ho ; Yang, Moon-Sik ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 97~102
DOI : 10.5010/JPB.2003.30.1.097
Lettuce (Lactuca sativa) was transformed with Agrobacterium tumefacience LBA4404 containing human granulocyte macrophage colony stimulating factor (hGM-CSF) gene to produce in cell suspension cultures. Cell suspension culture was established using callus from transgenic lettuce plant. Integration of hGM-CSF gene into plant chromosome was confirmed through genomic PCR and Southern blot analysis. In addition, Northern blot analysis indicated the expression of the introduced hGM-CSF gene in transformed lettuce. The recombinant hGM-CSF was expressed in transgenic cell cultures derived from transgenic plants as a yield of about 149.0
/L in culture filtrate, which was determined by ELISA. These results demonstrated that transformed lettuce cell suspension cultures could be used as a production system of therapeutic proteins such as hGM-CSF.
The γ-Benzenehexachloride Degradation Using Transgenic Tobacco Plant
Lee, Jeong-Kyung ; Park, Soon-Ki ; Chung, Il-Kyung ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 103~108
DOI : 10.5010/JPB.2003.30.1.103
LinA gene involving in the
-benzenehexachloride degradation have been cloned from Sphingmonas paucimobilis UT26. This linA gene which catalyzes the first dechlorination step of
-benzenehexachloride is known to play a key role in the
-benzenehexachloride degradation pathway in UT26. In this study, the linA gene was designed to clean-up the
-benzenehexachloride and its derivatives contaminated in soil, water and air using transgenic tobacco plants. The linA transgene was introduced into the chromosome of tobacco using leaf-disk transformation approach as revealed by Southern blot analysis. In addition, mRNA and protein produced by linA gene was expressed at a high level in the leaf tissue as demonstrated by both northern blot analysis and Western bolt analysis with polyclonal antibody against S. paucimobilis UT26. in vitro analysis using GC-MS showed that transgenic tobacco plant produced the linA protein which effectively degraded
- pentachlorocyclohexene and 1,2,4-trichlobenzene compounds which are less toxic.
Cryopreservation of Embryogenic Callus in Sweetpotato cv. `Yulmi`
Park, Jong-Suk ; Kim, Suk-Weon ; In, Dong-Su ; Eun, Jong-Seon ;
Journal of Plant Biotechnology, volume 30, issue 1, 2003, Pages 109~113
DOI : 10.5010/JPB.2003.30.1.109
Cryopreservation of embryogenic callus derived from apical meristem culture was attempted by slow prefreezing method (two-step method) with various cryoprotectants in sweetpotato cv. `Yulmi` Precultured embryogenic calli on medium containing 10 mg/L ABA prior to slow prefreezing in liquid nitrogen indicated higher survival rate than 1.0 mg/L ABA preteatment. The cryoprotectant comprising 1.28 M DMSO in 0.4 M sucrose solution gave the best survival (over 46%) of sweetpotato cells exposed to liquid nitrogen as determined by TTC reduction and FDA staining method. Cryopreserved calli cultured on MS medium with 1.0 mg/L 2,4-D were grown for 4 weeks in the dark and induced embryos after another 4 weeks. They were subcultured on MS medium supplemented with 0.1 mg/L 2,4-D＋0.1 mg/L kinetin for 2 weeks and regenerated into normal plantlets in MS basal medium.