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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 30, Issue 4 - Dec 2003
Volume 30, Issue 3 - Sep 2003
Volume 30, Issue 2 - Jun 2003
Volume 30, Issue 1 - Mar 2003
Selecting the target year
Molecular Cloning of Differentially Expressed Genes in First Trap Leaf of Dionaea muscipula by Fluorescent Differential Display
Kang, Kwon-Kyoo ; Lee, Keun-Hyang ; Park, Jin-Heui ; Hong, Kyong-Ei ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 307~313
DOI : 10.5010/JPB.2003.30.4.307
Fluorescent differential display (FDD) is a method for identifying differentially expressed genes in eukaryotic cells. The mRNA FDD technology works by systematic amplification of the 3` terminal regions of mRNAs. This method involve the reverse transcription using anchored primers designed to bind 5`boundary of the poly A tails, followed by polymerase chain reaction (PCR) amplification with additional upstream primers of arbitrary sequences. The amplified cDNA subpopulations are separated by denaturing polyacrylamide electrophoresis. To identify the genes involved in the development of first trap leaf, we applied a FDD method using mRNAs from leaf base, first trap leaf and flower tissue, respectively. We screened several genes that expressed specifically in first trap leaf. Nucleotide sequence analysis of these genes revealed that these were protease inhibitor (PI), myo-inositol-1-phosphate synthase and lipocalin-type prostaglandin D synthase. Northern blot analysis showed that these genes were expressed specifically in first trap leaf (in vivo and in vitro). FDD could prove to be useful for simultaneous scanning of transcripts from multiple cDNA samples and faster selection of differentially expressed transcripts of interest.
Transformation of Artemisia adamsii, Endemic to a Gobi Desert, with CLP, Dhn5 to Enhance Environmental Stress Tolerance
Han, Kyu-Hyun ; Hwang, Cheol-Ho ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 315~321
DOI : 10.5010/JPB.2003.30.4.315
Freezing and drought tolerances in plants are very important for survival in the desert. In an effort to reduce desertifcation in Gobi, a molecular breeding of Artemisia adamsii using the CLP (chitinase like protein, antifreeze protein) and Dhn5 (dehydrin5) genes from barley is performed by introducing them into Artemisia adamsii via Agrobacteria. We had found an optimal combinatorial concentration of hormones at 0.05mg/L of NAA and 0.5mg/L of BA for growth of callus in Artemisia adamsii. In addition, the higher rate of callus induction using hypocotyl as explant was observed comparing to explants of stem and leaf. There were some variations in the level of the proteins expressed among the transgenic lines such that the lines of CLP(CS1-5, 1-7,4-4) and Dhn5(DS2-2, 2-3) lines produce the protein to higher levels. The transgenic lines showing a higher level of Dhn5 exhibited better growth than nontransgenic callus in presence of 10 and 20％ PEG. In case of the CLP tansgenic lines, both CS1-5 and CS1-7 showed a higher level of freezing tolerance determined by ion leakage test.
Molecular Cloning and Characterization of Salt-inducible Aldolase from Salicornia herbacea
Cha, Joon-Yung ; Netty Ermawati ; Kim, Soon-Gil ; Lee, Jeung-Joo ; Lim, Chae-Oh ; Chung, Woo-Sik ; Lee, Kon-Ho ; Son, Dae-Young ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 323~328
DOI : 10.5010/JPB.2003.30.4.323
Soil salinity is one of the most serious abiotic stresses limiting the productivity of agricultural crops. To cope with salt stress, plants respond with physiological, developmental and biochemical changes, including the synthesis of a number of proteins and the induction of gene expression. Salicornia herbacea is a halophytic plant that grows in salt marshes and on muddy seashores. In order to understand the biochemical and molecular mechanisms of salt tolerance in S. herbacea, we isolated several genes that involved in the salt tolerance by mRNA differential display. Here we report the cloning of a cDNA encoding fructose-1, 6-bisphosphate aldolase, named ShADL, which is 1293 bp long and contains an open reading frame consisted of 359 amino acids with calculated molecular mass of 39 kDa. ShADL protein showed 86％ identity with Arabidopsis and 78％ with aldolase of common ice plant. Northern blot analysis revealed that the transcript of ShADL gene was increased dramatically depending on the NaCl concentrations.
Isolation and Characterization of a cDNA Encoding CycD3 Gene from Potato(Solanum tuberosum L.)
Kang, In-Hong ; Choi, Seung-Ho ; Lee, Hong-Geun ; Hwang, Hyun-Sik ; Lee, Suk-Chan ; Jung, Tae-Young ; Lim, Hak-Tae ; Bae, Shin-Chul ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 329~334
DOI : 10.5010/JPB.2003.30.4.329
D-type cyclins are believed to regulate the G1 to S phase transition in response to nutrient and hormonal signals. We investigated the expression characteristics of the key cell-cycle regulators, mitotic and G1 cyclins in potato (Solanum tuberosum L.). We isolated D-type cyclin gene from potato and it was classified as D3 cyclin by sequence similarities and a phylogenetic analysis, and named as StcycD3;1. The accumulation of transcripts was predominantly associated with mitotically active organs, such as stolons, roots, flowers, leaves, and stems. Transcription of StcycD3;1 can be induced by sucrose.
Introduction of LEAFY Gene to Chrysanthemum(Dendranthema x grandiflorum(Ramat.) Kitamura) ′Shuho-no-chikara′ Mediated by Agrobacterium LBA4404
Han, Bong-Hee ; Yae, Byeoung-Woo ; Yi, Sook-Yi ; Lee, Soo-Young ; Shin, Hack-Kee ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 335~339
DOI : 10.5010/JPB.2003.30.4.335
Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora `Shuho-no-chikara` by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days`early flowering in field.
High-frequency Plant Regeneration from Mature Seed-derived Callus Cultures of Orchardgrass
Lee, Sang-Hoon ; Lee, Dong-Gi ; Kim, Jin-Soo ; Lee, Byung-Hyun ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 341~346
DOI : 10.5010/JPB.2003.30.4.341
In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92％ when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61％. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69％. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.
Effect of Stigmatic and Cut-style Pollination on Selfed and Intraspecific Seed Set in Lilium spp.
Lee, Ji-Yong ; Lim, Yong-Pyo ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 347~352
DOI : 10.5010/JPB.2003.30.4.347
This work was undertaken to study the effect of stigmatic and cut-style pollination on self seed set in Lilium longiflorum and L.
formolongi, and their crosses as the female parent with other cultivars/genotypes. Stigmatic pollination of L. longiflorum cv. Gelria and Lorina resulted in cent per cent fruit set with mean number of seeds/fruit of 189 and 70, respectively. However, cut-style pollination resulted in 87％ and 40％ fruit set in Gelria and Lorina, respectively. The corresponding mean number of seeds/fruit was 53 and 20. In L.
formolongi, stigmatic pollination set 80％ fruits with 736 seeds/fruit. On the other hand, cut-style pollination resulted in 47％ fruit set with 81 seeds/fruit. The intraspecific stigmatic pollination of L. longiflorum cv. Gelria and Lorina with other cultivars formed 60％ fruits with a mean number of 18 seeds/fruit. However, only 20％ fruit set with mean number of seeds/fruit of 7 was recorded when cut-style pollination of L. longiflorum cultivar were pollinated with other cultivars/genotypes. In the intraspecific pollination of L.
formolongi cv. Raizan with Novia, fruit set resulting from stigmatic and cut-style pollination was 75％ and 50％, respectively with the corresponding mean number of seeds/fruit of 579 and 98. It was concluded that self as well as intraspecific seed set in the two species of Lilium gets considerably reduced as a result of cut-style pollination.
Seed Set in Close Pollination and Backcross of Interspecific F1 of Lilium spp.
Lee, Ji-Yong ; Lim, Yong-Pyo ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 353~357
DOI : 10.5010/JPB.2003.30.4.353
We studies seed set in the interspecific F1 /backcross hybrids of Lilium species. In the interspecfic hybrid of L. longiflorum cv. Gelria with L.
fomolongi cv. Raizan,93％ fruit set was obtained by stigmatic pollination in comparison to 53％ from cut-style pollination. Accordingly, the number of seed set resulting from stigmatic and cut-style pollination was 147 and 53, respectively. Pollination o( both stigmatic and cut-style pollination resulted in 47％ fruit set in the hybrid of L. longiflorum cv. Lorina with L.
fomolongi cv. Raizan. However, stigmatic pollination formed 413 seeds, whereas only 24 seeds were obtained by cut-style pollination in this cross. The hybrid of L.
fomolongi cv. Raizan with L. longiflorum cv. Come set 40％ fruit with a total of 43 seeds by stigmatic pollination. However, no fruit set was observed in cut-style pollination in this hybrid. Backcrossing the F1 hybrid by cut-style pollination of L. longiflorum cv. Lorina
Asiatic hybrid cv. Chicago with the latter parent led to 53％ fruit set, and 109 embryos were obtained. Likewise, backcrossing following cut-style pollination of L. longiflorum cv. Lorina
Asiatic hybrid cv. Corsia with the latter parent formed 67％ fruits and 107 embryos. However, in the remaining interspecific hybrids, cut-style pollination set no fruit.
Mass Propagation of Liriodendron tulipifera L. via Somatic Embryogenesis
Lee, Jae-Soon ; Moon, Heung-Kyu ; Kim, Yong-Wook ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 359~363
DOI : 10.5010/JPB.2003.30.4.359
Mass propagation of tulip tree (Liriodendron tulipifera L) via somatic embryogenesis was successfully achieved with immature samaras collected from adult trees. Embryogenic tissues were induced by culturing them samaras on 1/2 LM medium (Litvay`s) containing 2,4-D and BA. Somatic embryos developed from the embryogenic tissues and germinated to normal plants (emblings) upon transfer onto the same medium containing either AgNO
or activated charcoal. So far, several factors appeared to influence both the induction of embryogenic tissues and germination of the embryos into plants. These include the collection time of samaras for the induction of embryogenic tissue, sucrose level in the culture medium, the level of both AgNO
and activated charcoal, and plating density of somatic embryos on germination medium for maturation and germination of somatic embryos into plantlets.
Variations of Agronomic Traits on the Progenies of the Different Callus Origin and Subculture Period in Rice
Yi, Gi-Hwan ; Oh, Byeong-Geun ; Yang, Sae-Jun ; Kim, Soon-Chul ; Nam, Min-Hee ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 365~368
DOI : 10.5010/JPB.2003.30.4.365
Variations of agronomic traits were evaluated on the progenies of regenerated rice plants of different callus origin and subculture period. From eighty-eight percent to ninety percents of ovary culture-derived plants (OCP) and anther culture-derived plants (ACP) were not segregated within the lines in major agronomic characters. Compare to ACP and OCP lines, the seed culture derived lines (SCP) showed more segregation (17％) in major agronomic traits among the lines. The most frequent segregating traits were grain fertility (6.1％) in ACP, leaf color (4.2％) in OCP and grain fertility (4.9％) and leaf color (4.9％) in SCP lines. The SCP line showed more variation in culm length, panicle length and heading date than those of OCP and ACP lines. The variation of agronomic traits in SCP lines was tended to increase with prolonged subculture. Culm and panicle length were shorter than those of original cultivar in all three types of tissue culture-derived lines (OCP, ACP and SCP).
Effects of Cold Shock Pretreatment and Carbohydrate Sources on Anther Culture of Rice
Yi, Gi-Hwan ; Won, Yong-Jae ; Ko, Jong-Min ; Park, Hyang-Mi ; Cho, Jun-Hyeon ; Oh, Byeong-Geun ; Yang, Sae-Jun ; Kim, Soon-Chul ; Nam, Min-Hee ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 369~373
DOI : 10.5010/JPB.2003.30.4.369
In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.
Continuous Production of Phalaenopsis Clones by Basal Shoot Culture
Been, Chul-Gu ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 375~380
DOI : 10.5010/JPB.2003.30.4.375
This study was conducted to establish a practical masspropagation system of Phalaenopsis clones from basal shoot segments. The frequency of PLB (protocorm like body) induction was compared with various explants. Basal shoot segments showed the most successful result of 45％, while root tips, stalk node segments, stalk leaves and mature leaves represented low frequency (below 5％). The PLB induction ratio in the culture of basal shoot segments was examined with 11 different Phalaenopsis varieties, and the majority of varieties, including pink flower lines, showed an about 30％ rate of PLB formation. Especially, when whole basal shoot parts without cutting were inoculated onto PLB induction medium, giant PLB was induced from explant. This giant PLB was green color and big in size compared with normal PLB. When dissected giant PLB segments inoculated onto PLB multiplication medium, only normal size of PLBs were induced from them. PLBs induced by basal shoot culture were transferred onto proliferation medium and then shooting medium, from which normal plants were formed. Therefore, this culture method is considered as effective and practical protocol for Phalaenopsis mericlone production. In addition, it is suggested that clones of an infinite number can be produced consecutively by this culture system through repeated cycles of PLB induction and proliferation using the basal shoot segment of flask plant.
Plant Production from Desiccated Somatic Embryos of Acanthopanax chiisanensis
Lee, Kang-Seop ; Bang, Keuk-Soo ; Choi, Yong-Eui ; Ahn, Byung-Yong ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 381~385
DOI : 10.5010/JPB.2003.30.4.381
An efficient method of plant regeneration from Acanthopanax chiisanensis somatic embryos was developed. Cotyledonary somatic embryos were obtained in liquid Murashige and Skoog (MS) medium from embryogenic cell suspension cultures. They were desiccated for 0 to 72 hr and then cultured on MS medium containing NAA, BA, GA
, (0-0.5mg/L). The highest multiple shoots formation (100％) was obtained from 72 hr desiccated somatic embryos on ifs medium with 0.5mg/L NAA＋0.5mg/L BA or 0.5 mg/L NAA＋0.5mg/L BA＋0.5mg/L GA
after 6 weeks culture. Plant conversion from multiple shoots was not high. The highest plant conversion from multiple shoots was obtained on 1/3MS medium with 1.0mg/L GA
. Converted plantlets were transferred to ex vitro condition and the highest survival rate (70％) of the plantlets was obtained on plastic pots containing vermiculite and sand. These results indicate that micropropagation procedure can be applied for an efficient mass propagation of Acanthopanax chiisanensis.
Changes in the Concept of Nutrients and Transition of Plant Breeding
Harn, Chang-Yawl ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 387~397
DOI : 10.5010/JPB.2003.30.4.387
During the first half of twentieth century, even though the importance of non-calorie essential micronutrients of 13 vitamins and 17 minerals has been known to alleviate nutritional disorder; the primary objective of agriculture and plant breeding programs has been to increase the productivity and seed yields, and macronutrients of proteins, fats, and carbohydrates made up the bulk of foodstuff which were used primarily as an energy source. In the last decade it has been found that non-essential micronutrients encompass a vast group of phytochemicals including antioxidants that are not strictly required in the diet but when present at sufficient levels work as health-promoting chemicals. Nowadays agricultural crops are grown for health rather than for food or fiber, and modifying the nutritional compositions of plant foods has become an urgent health issue. To ensure an adequate intake of essential vitamins and minerals, and to increase the consumption of health-promoting phytochemicals, the researches on plant secondary metabolism have been made. The attempt to improve nutritional quality of crops has been blocked by a lack of basic knowledge of plant metabolism. The advent of genomics era enabled new approaches to make crossing regardless of species, family, or phylum barriers, and the accumulation in our basic knowledge on plant secondary metabolism during the coming decade would be tremendous. As the major staple crops contain insufficient amount of many micronutrients, fortification strategy will be a necessary practice. Elevated intake of specific vitamins, C, E, and
-carotene, mineral selenium, antioxidants, and phytochemicals significantly reduces the risk of chronic disease such as cancer, cardiovascular disorder, diabetis, and other degenerative disease associated with aging. As the attempt to improve the nutritional quality of crops requires the basic knowledges on plant metabolism, plant biochemistry, human physiology, and food chemistry, strong interdisplinary collaboration among plant biotechnologists, human nutritionists, and food scientists will be needed. Inhibition of cancer, cardiovascular disease, and other degenerative disorder may be the biggest goal facing nutritional plant breeders. But the assumption that simply increasing dietary level of any compound will necessarily improve human health is a dangerous idea because many plant secondary products and dietary contaminants have paradoxical (hermetic) effects. Before biotechnical manipulation is undertaken to elevate or reduce any individual constituent of crops, the contribution of the micronutrient to human health must first be investigated.
Photosynthetic Efficiency in Transgenic Tobacco Plants Expressing both CuZnSOD and APX in Chloroplasts against Oxidative Stress Caused by Highlight and Chilling
Kim, Yun-Hee ; Kwon, Suk-Yoon ; Bang, Jae-Wook ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 399~403
DOI : 10.5010/JPB.2003.30.4.399
In order to understand the protection effects of antioxidant enzymes against oxidative stress caused by various environmental stresses, transgenic tobacco (Nicotiana tabacum cv, Xanthi) plants expressing both copper/zinc superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts (referred to as CA plants) were subjected to highlight (1,100
) and chilling at 4
. The protection effects of CA plants using leaf discs were compared with those of transgenic plants expressing either CuZnSOD or APX in chloroplasts (SOD plants or APX plants, respectively) and non-transgenic (NT) plants. CA plants showed about 15％ protection in the photosynthetic efficiency (Fv/Fm) of photosystem II relative to NT plants 1 hr after treatment of both highlight and chilling, whereas they showed about 23％ protection in the redox state of P700 in photosystem I at 3 hr after treatment. SOD plants or APX plants showed an intermediate protection effect between CA plants and NT plants. These results demonstrated that the coexpression of CuZnSOD and APX in chloroplasts importantly involves in the protection effects against oxidative stress caused by various environmental stresses.
Susceptibility of Two Potato Cultivars to Various Environmental Stresses
Li, Tang ; Kwon, Suk-Yoon ; Kwak, Sang-Soo ; Sung, Chang-K ; Lee, Haeng-Soon ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 405~410
DOI : 10.5010/JPB.2003.30.4.405
Environmental stress is the major limiting factor in plant productivity. In order to evaluate the stress tolerance of potato plants, leaf discs of two potato cultivars, Atlantic and Superior, were subjected to various stress conditions of high temperature, methyl viologen, H2O2, or
. When potato leaf discs were exposed to high temperature at 37
for 84 hr, Atlantic plants, a cultivar with high sensitivity to heat stress, showed about 20％ higher membrane damage than Superior plants. When exposed to 2
M methyl violgen (MV), a superoxide generating non-selective herbicide, for 36 hr, Atlantic plants also showed about 38％ higher membrane damage than Superior plants, and were more susceptible up to 10
M MV concentration tested. On treatment with 0.75M NaCl, Atlantic plants also had about 45％ less chlorophyll contents in leaf discs than Superior plants. There was, however, no difference in chlorophyll content of two cultivars at higher NaCl concentrations. The effect of
on the two cultivars was mixed. At low
concentration (25 mM) , Superior plants were more susceptible to
stress after 36 hr. However, at high
concentration (100 mM), Atlantic plants exhibited higher susceptibility after 36 hr. The results indicate that in vitro leaf discs reflecting the whole plants in this study will be useful for selection and characterization of elite transgenic potato plants with enhanced tolerance to environmental stress.
Identification and Biosynthetic Pathway of Brassinosteroids in Seedling Shoots of Zea mays L.
Kang, Min-Wook ; Kim, Young-Soo ; Kim, Seong-Ki ;
Journal of Plant Biotechnology, volume 30, issue 4, 2003, Pages 411~419
DOI : 10.5010/JPB.2003.30.4.411
The potent biosynthetic precursors, 24
-methylcholesterol and 24
-methylcholestanol, and the endogenous brassinosteroids (BRs), castasterone (CS) and 6-deoxocastasterone (6-deoxoCS), were identified from shoots of maize seedlings. In addition, the presence for activities of several enzymes involved in the late C6-oxida-lion pathway from 24
-methylcholestanol to CS was demonstrated in the plants. However, activity for brassinolide (BL) synthase which catalyze the conversion of CS to BL, the last step of the late C6-oxidation pathway, was not detected in the enzyme solution obtained from the maize shoots. Together with the fact that BL was not identified from the maize shoots, these results strongly suggested that BRs in the maize shoots are biosynthesized during seedling growth and the active BR in the shoots is not BL but CS.